                         SEQUENCE LISTING

<110>  Garrison, Aura Rae
       Shoemaker, Charles Joseph
       Golden, Joseph Walter
        
<120>  GP38-TARGETING MONOCLONAL ANTIBODIES PROTECT ADULT MICE AGAINST 
       LETHAL CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS INFECTION

<130>  3000050-003977/RIID 18-21

<150>  62/789,576
<151>  2019-01-08

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1684
<212>  PRT
<213>  Orthonairovirus sp.

<400>  1

Met His Ile Ser Leu Met Tyr Ala Ile Leu Cys Leu Gln Leu Cys Gly 
1               5                   10                  15      


Leu Gly Glu Thr His Gly Ser His Asn Glu Thr Arg His Asn Lys Thr 
            20                  25                  30          


Asp Thr Met Thr Thr Pro Gly Asp Asn Pro Ser Ser Glu Pro Pro Val 
        35                  40                  45              


Ser Thr Ala Leu Ser Ile Thr Leu Asp Pro Ser Thr Val Thr Pro Thr 
    50                  55                  60                  


Thr Pro Ala Ser Gly Leu Glu Gly Ser Gly Glu Val Tyr Thr Ser Pro 
65                  70                  75                  80  


Pro Ile Thr Thr Gly Ser Leu Pro Leu Ser Glu Thr Thr Pro Glu Leu 
                85                  90                  95      


Pro Val Thr Thr Gly Thr Asp Thr Leu Ser Ala Gly Asp Val Asp Pro 
            100                 105                 110         


Ser Thr Gln Thr Ala Gly Gly Thr Ser Ala Pro Thr Val Arg Thr Ser 
        115                 120                 125             


Leu Pro Asn Ser Pro Ser Thr Pro Ser Thr Pro Gln Asp Thr His His 
    130                 135                 140                 


Pro Val Arg Asn Leu Leu Ser Val Thr Ser Pro Gly Pro Asp Glu Thr 
145                 150                 155                 160 


Ser Thr Pro Ser Gly Thr Gly Lys Glu Ser Ser Ala Thr Ser Ser Pro 
                165                 170                 175     


His Pro Val Ser Asn Arg Pro Pro Thr Pro Pro Ala Thr Ala Gln Gly 
            180                 185                 190         


Pro Thr Glu Asn Asp Ser His Asn Ala Thr Glu His Pro Glu Ser Leu 
        195                 200                 205             


Thr Gln Ser Ala Thr Pro Gly Leu Met Thr Ser Pro Thr Gln Ile Val 
    210                 215                 220                 


His Pro Gln Ser Ala Thr Pro Ile Thr Val Gln Asp Thr His Pro Ser 
225                 230                 235                 240 


Pro Thr Asn Arg Ser Lys Arg Asn Leu Lys Met Glu Ile Ile Leu Thr 
                245                 250                 255     


Leu Ser Gln Gly Leu Lys Lys Tyr Tyr Gly Lys Ile Leu Arg Leu Leu 
            260                 265                 270         


Gln Leu Thr Leu Glu Glu Asp Thr Glu Gly Leu Leu Glu Trp Cys Lys 
        275                 280                 285             


Arg Asn Leu Gly Leu Asp Cys Asp Asp Thr Phe Phe Gln Lys Arg Ile 
    290                 295                 300                 


Glu Glu Phe Phe Ile Thr Gly Glu Gly His Phe Asn Glu Val Leu Gln 
305                 310                 315                 320 


Phe Arg Thr Pro Gly Thr Leu Ser Thr Thr Glu Ser Thr Pro Ala Gly 
                325                 330                 335     


Leu Pro Thr Ala Glu Pro Phe Lys Ser Tyr Phe Ala Lys Gly Phe Leu 
            340                 345                 350         


Ser Ile Asp Ser Gly Tyr Tyr Ser Ala Lys Cys Tyr Ser Gly Thr Ser 
        355                 360                 365             


Asn Ser Gly Leu Gln Leu Ile Asn Ile Thr Arg His Ser Thr Arg Ile 
    370                 375                 380                 


Val Asp Thr Pro Gly Pro Lys Ile Thr Asn Leu Lys Thr Ile Asn Cys 
385                 390                 395                 400 


Ile Asn Leu Lys Ala Ser Ile Phe Lys Glu His Arg Glu Val Glu Ile 
                405                 410                 415     


Asn Val Leu Leu Pro Gln Val Ala Val Asn Leu Ser Asn Cys His Val 
            420                 425                 430         


Val Ile Lys Ser His Val Cys Asp Tyr Ser Leu Asp Ile Asp Gly Ala 
        435                 440                 445             


Val Arg Leu Pro His Ile Tyr His Glu Gly Val Phe Ile Pro Gly Thr 
    450                 455                 460                 


Tyr Lys Ile Val Ile Asp Lys Lys Asn Lys Leu Asn Asp Arg Cys Thr 
465                 470                 475                 480 


Leu Phe Thr Asp Cys Val Ile Lys Gly Arg Glu Val Arg Lys Gly Gln 
                485                 490                 495     


Ser Val Leu Arg Gln Tyr Lys Thr Glu Ile Arg Ile Gly Lys Ala Ser 
            500                 505                 510         


Thr Gly Ser Arg Arg Leu Leu Ser Glu Glu Pro Ser Asp Asp Cys Ile 
        515                 520                 525             


Ser Arg Thr Gln Leu Leu Arg Thr Glu Thr Ala Glu Ile His Gly Asp 
    530                 535                 540                 


Asn Tyr Gly Gly Pro Gly Asp Lys Ile Thr Ile Cys Asn Gly Ser Thr 
545                 550                 555                 560 


Ile Val Asp Gln Arg Leu Gly Ser Glu Leu Gly Cys Tyr Thr Ile Asn 
                565                 570                 575     


Arg Val Arg Ser Phe Lys Leu Cys Glu Asn Ser Ala Thr Gly Lys Asn 
            580                 585                 590         


Cys Glu Ile Asp Ser Val Pro Val Lys Cys Arg Gln Gly Tyr Cys Leu 
        595                 600                 605             


Arg Ile Thr Gln Glu Gly Arg Gly His Val Lys Leu Ser Arg Gly Ser 
    610                 615                 620                 


Glu Val Val Leu Asp Ala Cys Asp Thr Ser Cys Glu Ile Met Ile Pro 
625                 630                 635                 640 


Lys Gly Thr Gly Asp Ile Leu Val Asp Cys Ser Gly Gly Gln Gln His 
                645                 650                 655     


Phe Leu Lys Asp Asn Leu Ile Asp Leu Gly Cys Pro Lys Ile Pro Leu 
            660                 665                 670         


Leu Gly Lys Met Ala Ile Tyr Ile Cys Arg Met Ser Asn His Pro Lys 
        675                 680                 685             


Thr Thr Met Ala Phe Leu Phe Trp Phe Ser Phe Gly Tyr Val Ile Thr 
    690                 695                 700                 


Cys Ile Leu Cys Lys Ala Ile Phe Tyr Leu Leu Ile Ile Val Gly Thr 
705                 710                 715                 720 


Leu Gly Lys Arg Leu Lys Gln Tyr Arg Glu Leu Lys Pro Gln Thr Cys 
                725                 730                 735     


Thr Ile Cys Glu Thr Thr Pro Val Asn Ala Ile Asp Ala Glu Met His 
            740                 745                 750         


Asp Leu Asn Cys Ser Tyr Asn Ile Cys Pro Tyr Cys Ala Ser Arg Leu 
        755                 760                 765             


Thr Ser Asp Gly Leu Ala Arg His Val Ile Gln Cys Pro Lys Arg Lys 
    770                 775                 780                 


Glu Lys Val Glu Glu Thr Glu Leu Tyr Leu Asn Leu Glu Arg Ile Pro 
785                 790                 795                 800 


Trp Val Val Arg Lys Leu Leu Gln Val Ser Glu Ser Thr Gly Val Ala 
                805                 810                 815     


Leu Lys Arg Ser Ser Trp Leu Ile Val Leu Leu Val Leu Phe Thr Val 
            820                 825                 830         


Ser Leu Ser Pro Val Gln Ser Ala Pro Ile Gly Gln Gly Lys Thr Ile 
        835                 840                 845             


Glu Ala Tyr Arg Ala Arg Glu Gly Tyr Thr Ser Ile Cys Leu Phe Val 
    850                 855                 860                 


Leu Gly Ser Ile Leu Phe Ile Val Ser Cys Leu Met Lys Gly Leu Val 
865                 870                 875                 880 


Asp Ser Val Gly Asn Ser Phe Phe Pro Gly Leu Ser Ile Cys Lys Thr 
                885                 890                 895     


Cys Ser Ile Ser Ser Ile Asn Gly Phe Glu Ile Glu Ser His Lys Cys 
            900                 905                 910         


Tyr Cys Ser Leu Phe Cys Cys Pro Tyr Cys Arg His Cys Ser Thr Asp 
        915                 920                 925             


Lys Glu Ile His Lys Leu His Leu Ser Ile Cys Lys Lys Arg Lys Lys 
    930                 935                 940                 


Gly Ser Asn Val Met Leu Ala Val Cys Lys Leu Met Cys Phe Arg Ala 
945                 950                 955                 960 


Thr Met Glu Val Ser Asn Arg Ala Leu Phe Ile Arg Ser Ile Ile Asn 
                965                 970                 975     


Thr Thr Phe Val Leu Cys Ile Leu Ile Leu Ala Val Cys Val Val Ser 
            980                 985                 990         


Thr Ser Ala Val Glu Met Glu Asn  Leu Pro Ala Gly Thr  Trp Glu Arg 
        995                 1000                 1005             


Glu Glu  Asp Leu Thr Asn Phe  Cys His Gln Glu Cys  Gln Val Thr 
    1010                 1015                 1020             


Glu Thr  Glu Cys Leu Cys Pro  Tyr Glu Ala Leu Val  Leu Arg Lys 
    1025                 1030                 1035             


Pro Leu  Phe Leu Asp Ser Thr  Ala Lys Gly Met Lys  Asn Leu Leu 
    1040                 1045                 1050             


Asn Ser  Thr Ser Leu Glu Thr  Ser Leu Ser Ile Glu  Ala Pro Trp 
    1055                 1060                 1065             


Gly Ala  Ile Asn Val Gln Ser  Thr Tyr Lys Pro Thr  Val Ser Thr 
    1070                 1075                 1080             


Ala Asn  Ile Ala Leu Ser Trp  Ser Ser Val Glu His  Arg Gly Asn 
    1085                 1090                 1095             


Lys Ile  Leu Val Ser Gly Arg  Ser Glu Ser Ile Met  Lys Leu Glu 
    1100                 1105                 1110             


Glu Arg  Thr Gly Ile Ser Trp  Asp Leu Gly Val Glu  Asp Ala Ser 
    1115                 1120                 1125             


Glu Ser  Lys Leu Leu Thr Val  Ser Val Met Asp Leu  Ser Gln Met 
    1130                 1135                 1140             


Tyr Ser  Pro Val Phe Glu Tyr  Leu Ser Gly Asp Arg  Gln Val Gly 
    1145                 1150                 1155             


Glu Trp  Pro Lys Ala Thr Cys  Thr Gly Asp Cys Pro  Glu Arg Cys 
    1160                 1165                 1170             


Gly Cys  Thr Ser Ser Thr Cys  Leu His Lys Glu Trp  Pro His Ser 
    1175                 1180                 1185             


Arg Asn  Trp Arg Cys Asn Pro  Thr Trp Cys Trp Gly  Val Gly Thr 
    1190                 1195                 1200             


Gly Cys  Thr Cys Cys Gly Leu  Asp Val Lys Asp Leu  Phe Thr Asp 
    1205                 1210                 1215             


Tyr Met  Phe Val Lys Trp Lys  Val Glu Tyr Ile Lys  Thr Glu Ala 
    1220                 1225                 1230             


Ile Val  Cys Val Glu Leu Thr  Ser Gln Glu Arg Gln  Cys Ser Leu 
    1235                 1240                 1245             


Ile Glu  Ala Gly Thr Arg Phe  Asn Leu Gly Pro Val  Thr Ile Thr 
    1250                 1255                 1260             


Leu Ser  Glu Pro Arg Asn Ile  Gln Gln Lys Leu Pro  Pro Glu Ile 
    1265                 1270                 1275             


Ile Thr  Leu His Pro Arg Ile  Glu Glu Gly Phe Phe  Asp Leu Met 
    1280                 1285                 1290             


His Val  Gln Lys Val Leu Ser  Ala Ser Thr Val Cys  Lys Leu Gln 
    1295                 1300                 1305             


Ser Cys  Thr His Gly Val Pro  Gly Asp Leu Gln Val  Tyr His Ile 
    1310                 1315                 1320             


Gly Asn  Leu Leu Lys Gly Asp  Lys Val Asn Gly His  Leu Ile His 
    1325                 1330                 1335             


Lys Ile  Glu Pro His Phe Asn  Thr Ser Trp Met Ser  Trp Asp Gly 
    1340                 1345                 1350             


Cys Asp  Leu Asp Tyr Tyr Cys  Asn Met Gly Asp Trp  Pro Ser Cys 
    1355                 1360                 1365             


Thr Tyr  Thr Gly Val Thr Gln  His Asn His Ala Ser  Phe Val Asn 
    1370                 1375                 1380             


Leu Leu  Asn Ile Glu Thr Asp  Tyr Thr Lys Asn Phe  His Phe His 
    1385                 1390                 1395             


Ser Lys  Arg Val Thr Ala His  Gly Asp Thr Pro Gln  Leu Asp Leu 
    1400                 1405                 1410             


Lys Ala  Arg Pro Thr Tyr Gly  Ala Gly Glu Ile Thr  Val Leu Val 
    1415                 1420                 1425             


Glu Val  Ala Asp Met Glu Leu  His Thr Lys Lys Ile  Glu Ile Ser 
    1430                 1435                 1440             


Gly Leu  Lys Phe Ala Ser Leu  Ala Cys Thr Gly Cys  Tyr Ala Cys 
    1445                 1450                 1455             


Ser Ser  Gly Ile Ser Cys Lys  Val Arg Ile His Val  Asp Glu Pro 
    1460                 1465                 1470             


Asp Glu  Leu Thr Val His Val  Lys Ser Asp Asp Pro  Asp Val Val 
    1475                 1480                 1485             


Ala Ala  Ser Ser Ser Leu Met  Ala Arg Lys Leu Glu  Phe Gly Thr 
    1490                 1495                 1500             


Asp Ser  Thr Phe Lys Ala Phe  Ser Ala Met Pro Lys  Thr Ser Leu 
    1505                 1510                 1515             


Cys Phe  Tyr Ile Val Glu Arg  Glu His Cys Lys Ser  Cys Ser Glu 
    1520                 1525                 1530             


Glu Asp  Thr Lys Lys Cys Val  Asn Thr Lys Leu Glu  Gln Pro Gln 
    1535                 1540                 1545             


Ser Ile  Leu Ile Glu His Lys  Gly Thr Ile Ile Gly  Lys Gln Asn 
    1550                 1555                 1560             


Ser Thr  Cys Thr Ala Lys Ala  Ser Cys Trp Leu Glu  Ser Val Lys 
    1565                 1570                 1575             


Ser Phe  Phe Tyr Gly Leu Lys  Asn Met Leu Ser Gly  Ile Phe Gly 
    1580                 1585                 1590             


Asn Val  Phe Met Gly Ile Phe  Leu Phe Leu Ala Pro  Phe Ile Leu 
    1595                 1600                 1605             


Leu Ile  Leu Phe Phe Met Phe  Gly Trp Arg Ile Leu  Phe Cys Phe 
    1610                 1615                 1620             


Lys Cys  Cys Arg Arg Thr Arg  Gly Leu Phe Lys Tyr  Arg His Leu 
    1625                 1630                 1635             


Lys Asp  Asp Glu Glu Thr Gly  Tyr Arg Arg Ile Ile  Glu Lys Leu 
    1640                 1645                 1650             


Asn Asn  Lys Lys Gly Lys Asn  Lys Leu Leu Asp Gly  Glu Arg Leu 
    1655                 1660                 1665             


Ala Asp  Arg Arg Ile Ala Glu  Leu Phe Ser Thr Lys  Thr His Ile 
    1670                 1675                 1680             


Gly 
    


<210>  2
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  tPA secretion signal sequence

<400>  2

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Ser 
            20              


<210>  3
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  deltaMUC forward primer

<400>  3
gatctccatc ttcaggttgt ggctgccgtg ggtctc                                 36


<210>  4
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  deltaMUC reverse primer

<400>  4
gagacccacg gcagccacaa cctgaagatg gagatc                                 36


<210>  5
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  deltaMUC deltaGP38 forward primer

<400>  5
atcgctgggc tcctcgctgt ggctgccgtg ggtctc                                 36


<210>  6
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  deltaMUC deltaGP38 reverse primer

<400>  6
gagacccacg gcagccacag cgaggagccc agcgat                                 36


