                         SEQUENCE LISTING

<110>  REGENERON PHARMACEUTICALS, INC.
 
<120>  TRACELESS LINKERS AND PROTEIN-CONJUGATES THEREOF

<130>  114581.00298

<140>  PCT/US2020/012798
<141>  2020-01-08

<150>  US 62/872,229
<151>  2019-07-09

<150>  US 62/937,721
<151>  2019-11-19

<150>  PCT/US2019/012786
<151>  2019-01-08

<150>  US 16/243,020
<151>  2019-01-08

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, HCVR

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Ile Ser Ser Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Gly Asn Lys Tyr Ser Val Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, HCDR1

<400>  2

Gly Phe Thr Ile Ser Ser Tyr Gly 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, HCDR2

<400>  3

Ile Ser Tyr Asp Gly Gly Asn Lys 
1               5               


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, HCDR3

<400>  4

Ala Arg Gly Arg Tyr Phe Asp Leu 
1               5               


<210>  5
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, LCVR

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Arg Ala Pro Asn Leu Leu Ile 
        35                  40                  45              


Ser Lys Ala Ser Ser Leu Lys Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Val Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Ser Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, LCDR1

<400>  6

Gln Ser Ile Ser Ser Trp 
1               5       


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, LCDR2

<400>  7

Lys Ala Ser 
1           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H7814N, LCDR3

<400>  8

Gln Gln Tyr Tyr Ser Tyr Ser Tyr Thr 
1               5                   


<210>  9
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, HCVR

<400>  9

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Gly Ala Ser Gly Phe Thr Phe Arg Asn Tyr 
            20                  25                  30          


Gly Met Gln Trp Val Arg Gln Gly Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Thr Leu Ile Ser Phe Asp Gly Asn Asp Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Thr Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, HCDR1

<400>  10

Gly Phe Thr Phe Arg Asn Tyr Gly 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, HCDR2

<400>  11

Ile Ser Phe Asp Gly Asn Asp Lys 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, HCDR3

<400>  12

Ala Arg Gly Gly Asp Phe Asp Tyr 
1               5               


<210>  13
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, LCVR

<400>  13

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Lys Asp 
            20                  25                  30          


Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln His Asn Ser Tyr Pro Met 
                85                  90                  95      


Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105             


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, LCDR1

<400>  14

Gln Asp Ile Arg Lys Asp 
1               5       


<210>  15
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, LCDR2

<400>  15

Ala Ala Ser 
1           


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H6958N2, LCDR3

<400>  16

Leu Gln His Asn Ser Tyr Pro Met Tyr Thr 
1               5                   10  


<210>  17
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hPRLR ecto-MMH,

<400>  17

Met His Arg Pro Arg Arg Arg Gly Thr Arg Pro Pro Pro Leu Ala Leu 
1               5                   10                  15      


Leu Ala Ala Leu Leu Leu Ala Ala Arg Gly Ala Asp Ala Gln Leu Pro 
            20                  25                  30          


Pro Gly Lys Pro Glu Ile Phe Lys Cys Arg Ser Pro Asn Lys Glu Thr 
        35                  40                  45              


Phe Thr Cys Trp Trp Arg Pro Gly Thr Asp Gly Gly Leu Pro Thr Asn 
    50                  55                  60                  


Tyr Ser Leu Thr Tyr His Arg Glu Gly Glu Thr Leu Met His Glu Cys 
65                  70                  75                  80  


Pro Asp Tyr Ile Thr Gly Gly Pro Asn Ser Cys His Phe Gly Lys Gln 
                85                  90                  95      


Tyr Thr Ser Met Trp Arg Thr Tyr Ile Met Met Val Asn Ala Thr Asn 
            100                 105                 110         


Gln Met Gly Ser Ser Phe Ser Asp Glu Leu Tyr Val Asp Val Thr Tyr 
        115                 120                 125             


Ile Val Gln Pro Asp Pro Pro Leu Glu Leu Ala Val Glu Val Lys Gln 
    130                 135                 140                 


Pro Glu Asp Arg Lys Pro Tyr Leu Trp Ile Lys Trp Ser Pro Pro Thr 
145                 150                 155                 160 


Leu Ile Asp Leu Lys Thr Gly Trp Phe Thr Leu Leu Tyr Glu Ile Arg 
                165                 170                 175     


Leu Lys Pro Glu Lys Ala Ala Glu Trp Glu Ile His Phe Ala Gly Gln 
            180                 185                 190         


Gln Thr Glu Phe Lys Ile Leu Ser Leu His Pro Gly Gln Lys Tyr Leu 
        195                 200                 205             


Val Gln Val Arg Cys Lys Pro Asp His Gly Tyr Trp Ser Ala Trp Ser 
    210                 215                 220                 


Pro Ala Thr Phe Ile Gln Ile Pro Ser Asp Phe Thr Met Asn Asp Glu 
225                 230                 235                 240 


Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly Gly Glu Gln Lys Leu Ile 
                245                 250                 255     


Ser Glu Glu Asp Leu His His His His His His 
            260                 265         


<210>  18
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCVR

<400>  18
caggtgcagc tgcaggagtc gggcccagga ctggtgaagc cttcggagac cctgtccctc       60

acctgcactg tcactggtgg ctccatcagt aggaactact ggagttggat ccggcagccc      120

ccagggaagg gactggaatg gattggatat atctattaca gtgggagtat cgactacaat      180

ccctccctca agagtcgagt caccatatca gtagacacgt ccaagaacca gttctccctg      240

aagctgagtt ctatgaccgc tgcggacacg gccgtatact actgtgcgag agatcggtgg      300

aactggaaat acggtatgga cgtctggggc caagggacca cggtcatcgt ctcgtca         357


<210>  19
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCVR

<400>  19

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Gly Ser Ile Ser Arg Asn 
            20                  25                  30          


Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Tyr Ser Gly Ser Ile Asp Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Lys Leu Ser Ser Met Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Arg Trp Asn Trp Lys Tyr Gly Met Asp Val Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Ile Val Ser Ser 
        115                 


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR1

<400>  20
ggtggctcca tcagtaggaa ctac                                              24


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR1

<400>  21

Gly Gly Ser Ile Ser Arg Asn Tyr 
1               5               


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR2

<400>  22
atctattaca gtgggagtat c                                                 21


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR2

<400>  23

Ile Tyr Tyr Ser Gly Ser Ile 
1               5           


<210>  24
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR3

<400>  24
gcgagagatc ggtggaactg gaaatacggt atggacgtc                              39


<210>  25
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, HCDR3

<400>  25

Ala Arg Asp Arg Trp Asn Trp Lys Tyr Gly Met Asp Val 
1               5                   10              


<210>  26
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCVR

<400>  26
gaaattgtgt tgacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc       60

ctctcctgca gggccagtca gactgttaga aacaactact tagcctggta ccaccagaaa      120

cctggccagg ctcccaggct cctcatctat ggtgcatcca gcagggccac tggcatccca      180

gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag      240

cctgaagatt ttacagtgta ttactgtcac cagtatggta actcaccttg gacgttcggc      300

caagggacca aaatggaaat caaacga                                          327


<210>  27
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCVR

<400>  27

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Thr Val Arg Asn Asn 
            20                  25                  30          


Tyr Leu Ala Trp Tyr His Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Thr Val Tyr Tyr Cys His Gln Tyr Gly Asn Ser Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Met Glu Ile Lys Arg 
            100                 105                 


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR1

<400>  28
cagactgtta gaaacaacta c                                                 21


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR1

<400>  29

Gln Thr Val Arg Asn Asn Tyr 
1               5           


<210>  30
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR2

<400>  30
ggtgcatcc                                                                9


<210>  31
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR2

<400>  31

Gly Ala Ser 
1           


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR3

<400>  32
caccagtatg gtaactcacc ttggacg                                           27


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: H1H21234N, LCDR3

<400>  33

His Gln Tyr Gly Asn Ser Pro Trp Thr 
1               5                   


