                         SEQUENCE LISTING

<110>  GEN-PROBE INCORPORATED
       JOHNSON, Tamara
       DARBY, Paul M
       GETMAN, Damon K
 
<120>  DETECTION OF DRUG-RESISTANT MYCOPLASMA GENITALIUM

<130>  DIA.0064-PCT

<140>  TBA
<141>  2020-01-23

<150>  US 62/797,053
<151>  2019-01-25

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  Forward primer

<400>  1
ctcggctata gactcggtga aatcc                                             25


<210>  2
<211>  14
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Reverse primer

<400>  2
tacatggtgg tgtt                                                         14


<210>  3
<211>  15
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Reverse primer

<400>  3
ctacatggtg gtgtt                                                        15


<210>  4
<211>  17
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  Reverse primer

<400>  4
ctctacatgg tggtgtt                                                      17


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  5' sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (7)..(23)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  5
ccacgtctct acatggtggt gtt                                               23


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  5' sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (7)..(23)
<223>  Sequence complementary to ribosomal nucleic acid of  Mycoplasma 
       genitalium

<400>  6
cctattctct acatggtggt gtt                                               23


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  5' sequence not complementary to nucleic acid of Mycoplasma 
       genitalium

<220>
<221>  misc_feature
<222>  (7)..(23)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  7
ggtgttctct acatggtggt gtt                                               23


<210>  8
<211>  16
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  Invasive probe

<400>  8
ggcgcaacgg gacggg                                                       16


<210>  9
<211>  17
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  Invasive probe

<400>  9
ggcgcaacgg gacgggt                                                      17


<210>  10
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primary probe based on wild-type Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  5'-flap equence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (13)..(34)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  10
acggacgcgg agtttgatca atattaagct acag                                   34


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primary probe based on macrolide-resistant Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  5'-flap sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (11)..(26)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  11
cgcgccgagg agagaccccg tgaagc                                            26


<210>  12
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primary probe based on macrolide-resistant Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  5'-flap sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (11)..(26)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  12
cgcgccgagg gaagaccccg tgaagc                                            26


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primary probe based on macrolide-resistant Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  5'-flap sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (11)..(26)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  13
cgcgccgagg caagaccccg tgaagc                                            26


<210>  14
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primary probe based on macrolide-resistant Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  5'-flap sequence not complementary to ribosomal nucleic acid of 
       Mycoplasma genitalium

<220>
<221>  misc_feature
<222>  (11)..(26)
<223>  Sequence complementary to ribosomal nucleic acid of Mycoplasma 
       genitalium

<400>  14
cgcgccgagg taagaccccg tgaagc                                            26


<210>  15
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET cassette to detect wild-type ribosomal sequences in 
       Mycoplasma genitalium

<400>  15
tctagccggt tttccggctg agactccgcg tccgt                                  35


<210>  16
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET cassette to detect macrolide resistance sequences in 
       Mycoplasma genitalium

<400>  16
tctagccggt tttccggctg agacctcggc gcg                                    33


<210>  17
<211>  24
<212>  RNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Target-binding sequence of target capture oligonucleotide

<400>  17
cccucguucu ucacugcggc uuac                                              24


<210>  18
<211>  24
<212>  RNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Target-binding sequence of target capture oligonucleotide

<400>  18
cauauacauc accuuacggu uugg                                              24


<210>  19
<211>  21
<212>  RNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  Target-binding sequence of target capture oligonucleotide

<400>  19
ccuuugugcg ccuccguuac c                                                 21


<210>  20
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  Target capture oligonucleotide

<400>  20
cccucguucu ucacugcggc uuactttaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa          57


<210>  21
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  Target capture oligonucleotide

<400>  21
cauauacauc accuuacggu uuggtttaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa          57


<210>  22
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Target capture oligonucleotide

<400>  22
ccuuugugcg ccuccguuac ctttaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa             54


<210>  23
<211>  691
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(691)
<223>  Wild-type Mycoplasma genitalium 23S ribosomal nucleic acid 
       sequence

<400>  23
tctatgcttc acggaattcg gcaattaatc atgctttcaa gaaaagctgc tagggctaat       60

ttaactgtaa ccagtaccga gaacgaacac acgtagtcaa ggagaggatc ctaaggttag      120

cgagtgaact atagccaagg aactctgcaa attaaccccg taagttagcg agaaggggtg      180

cttatctaaa agtaagccgc agtgaagaac gaggggggac tgtttaacta aaacacaact      240

ctatgccaaa ccgtaaggtg atgtatatgg ggtgacacct gcccagtgct ggaaggttaa      300

agaaggaggt tagcaattta ttgcaaagct tttaactgaa gccccagtga acggcggccg      360

taactataac ggtcctaagg tagcgaaatt cctagtcggg taaattccgt cccgcttgaa      420

tggtgtaacc atctcttgac tgtctcggct atagactcgg tgaaatccag gtacgggtga      480

agacacccgt taggcgcaac gggacggaaa gaccccgtga agctttactg tagcttaata      540

ttgatcaaaa caccaccatg tagagaatag gtaggagcaa ttgatgcaag ttcgcaagga      600

tttgttgatg tgaaatgtgg aatactaccc ttggttatgt tttgttctaa ttggtaactg      660

tgatccaggc ggccgctgct acactggatc c                                     691


<210>  24
<211>  691
<212>  DNA
<213>  Mycoplasma genitalium


<220>
<221>  misc_feature
<222>  (1)..(691)
<223>  Macrolide-resistant Mycoplasma genitalium 23S ribosomal nucleic 
       acid sequence

<220>
<221>  misc_feature
<222>  (509)..(509)
<223>  The G nucleotide at position 509 is characteristic of macrolide 
       resistance

<400>  24
tctatgcttc acggaattcg gcaattaatc atgctttcaa gaaaagctgc tagggctaat       60

ttaactgtaa ccagtaccga gaacgaacac acgtagtcaa ggagaggatc ctaaggttag      120

cgagtgaact atagccaagg aactctgcaa attaaccccg taagttagcg agaaggggtg      180

cttatctaaa agtaagccgc agtgaagaac gaggggggac tgtttaacta aaacacaact      240

ctatgccaaa ccgtaaggtg atgtatatgg ggtgacacct gcccagtgct ggaaggttaa      300

agaaggaggt tagcaattta ttgcaaagct tttaactgaa gccccagtga acggcggccg      360

taactataac ggtcctaagg tagcgaaatt cctagtcggg taaattccgt cccgcttgaa      420

tggtgtaacc atctcttgac tgtctcggct atagactcgg tgaaatccag gtacgggtga      480

agacacccgt taggcgcaac gggacggaga gaccccgtga agctttactg tagcttaata      540

ttgatcaaaa caccaccatg tagagaatag gtaggagcaa ttgatgcaag ttcgcaagga      600

tttgttgatg tgaaatgtgg aatactaccc ttggttatgt tttgttctaa ttggtaactg      660

tgatccaggc ggccgctgct acactggatc c                                     691


