                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       Muthumani, Kar
       Weiner, David
       Perales-Puchalt, Alfredo
       Duperret, Elizabeth
 
<120>  DNA-ENCODED BISPECIFIC T-CELL ENGAGERS TARGETING CANCER ANTIGENS 
       AND METHODS OF USE IN CANCER THERAPUTICS

<130>  206194-0033-00WO

<150>  US 62/798,626
<151>  2019-01-30

<150>  US 62/827,265
<151>  2019-04-01

<160>  76    

<170>  PatentIn version 3.5

<210>  1
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, without start or IgE leader

<400>  1
gatattcagc tgactcagag ccctgcttcc ctggccgtgt ccctgggaca gagggcaacc       60

atctcttgca aggcctccca gtctgtggac tacgatggcg actcctatct gaactggtac      120

cagcagatcc caggccagcc ccctaagctg ctgatctatg acgcctctaa tctggtgagc      180

ggcatcccac ccagattcag cggctccggc tctggcacag acttcaccct gaacatccac      240

ccagtggaga aggtggacgc agcaacctac cactgccagc agtccacaga ggatccttgg      300

accttcggcg gcggcacaaa gctggagatc aagggcggcg gcggcagcgg cggcggcggc      360

agcggcggcg gcggcagcca ggtgcagctg cagcagagcg gcgccgagct ggtgcggccc      420

ggcagctccg tgaagatctc ctgtaaggcc tctggctatg ccttttctag ctactggatg      480

aattgggtga agcagcgccc cggacagggc ctggagtgga tcggccagat ctggcctggc      540

gatggcgaca caaactacaa tggcaagttc aagggcaagg ccacactgac cgccgacgag      600

tcctctagca ccgcctacat gcagctgtcc tctctggcct ccgaggattc tgccgtgtat      660

ttttgcgcca ggagagagac aacaaccgtg ggcaggtact attacgccat ggactactgg      720

ggccagggca caaccgtgac agtgagcagc ggcggcggcg gctctgatat caagctgcag      780

cagagcggag cagagctggc cagacctgga gccagcgtga agatgtcctg taagacctct      840

ggctatacat tcacccggta cacaatgcac tgggtgaagc agcgcccagg ccagggcctg      900

gaatggatcg gctacatcaa ccccagccgg ggctatacca actacaatca gaagtttaag      960

gataaggcca ccctgacaac cgacaagtct agctccacag cctatatgca gctgtctagc     1020

ctgaccagcg aggactccgc cgtgtattac tgcgcccgct attacgacga tcactattgt     1080

ctggattatt ggggacaggg cacaaccctg acagtgtcct ctgtggaggg cggctctggc     1140

ggcagcggcg gctccggcgg ctctggcggc gtggacgata tccagctgac ccagtcccct     1200

gcaatcatga gcgcctcccc aggagagaag gtgacaatga cctgcagggc cagctcctct     1260

gtgagctata tgaattggta ccagcagaag agcggcacat cccctaagag gtggatctat     1320

gacaccagca aggtggcctc cggcgtgcca tacagattct ctggcagcgg ctccggcacc     1380

tcttatagcc tgacaatcag ctccatggag gccgaggatg ccgccaccta ttactgtcag     1440

cagtggtcta gcaatcctct gactttcggg gctgggacaa aactggaact gaag           1494


<210>  2
<211>  498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, without start or IgE leader

<400>  2

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser Ser Val 
    130                 135                 140                 


Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr Trp Met 
145                 150                 155                 160 


Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Gln 
                165                 170                 175     


Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys Gly 
            180                 185                 190         


Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr Met Gln 
        195                 200                 205             


Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 
    210                 215                 220                 


Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp 
                245                 250                 255     


Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser 
            260                 265                 270         


Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr 
        275                 280                 285             


Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly 
    290                 295                 300                 


Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
305                 310                 315                 320 


Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met 
                325                 330                 335     


Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala 
            340                 345                 350         


Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr 
        355                 360                 365             


Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly 
    370                 375                 380                 


Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro 
385                 390                 395                 400 


Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg 
                405                 410                 415     


Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly 
            420                 425                 430         


Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly 
        435                 440                 445             


Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
    450                 455                 460                 


Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
465                 470                 475                 480 


Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu 
                485                 490                 495     


Leu Lys 
        


<210>  3
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, operably linked to IgE leader

<400>  3
atggactgga cttggatcct gttcctggtc gccgccgcca cacgggtgca ttccgatatt       60

cagctgactc agagccctgc ttccctggcc gtgtccctgg gacagagggc aaccatctct      120

tgcaaggcct cccagtctgt ggactacgat ggcgactcct atctgaactg gtaccagcag      180

atcccaggcc agccccctaa gctgctgatc tatgacgcct ctaatctggt gagcggcatc      240

ccacccagat tcagcggctc cggctctggc acagacttca ccctgaacat ccacccagtg      300

gagaaggtgg acgcagcaac ctaccactgc cagcagtcca cagaggatcc ttggaccttc      360

ggcggcggca caaagctgga gatcaagggc ggcggcggca gcggcggcgg cggcagcggc      420

ggcggcggca gccaggtgca gctgcagcag agcggcgccg agctggtgcg gcccggcagc      480

tccgtgaaga tctcctgtaa ggcctctggc tatgcctttt ctagctactg gatgaattgg      540

gtgaagcagc gccccggaca gggcctggag tggatcggcc agatctggcc tggcgatggc      600

gacacaaact acaatggcaa gttcaagggc aaggccacac tgaccgccga cgagtcctct      660

agcaccgcct acatgcagct gtcctctctg gcctccgagg attctgccgt gtatttttgc      720

gccaggagag agacaacaac cgtgggcagg tactattacg ccatggacta ctggggccag      780

ggcacaaccg tgacagtgag cagcggcggc ggcggctctg atatcaagct gcagcagagc      840

ggagcagagc tggccagacc tggagccagc gtgaagatgt cctgtaagac ctctggctat      900

acattcaccc ggtacacaat gcactgggtg aagcagcgcc caggccaggg cctggaatgg      960

atcggctaca tcaaccccag ccggggctat accaactaca atcagaagtt taaggataag     1020

gccaccctga caaccgacaa gtctagctcc acagcctata tgcagctgtc tagcctgacc     1080

agcgaggact ccgccgtgta ttactgcgcc cgctattacg acgatcacta ttgtctggat     1140

tattggggac agggcacaac cctgacagtg tcctctgtgg agggcggctc tggcggcagc     1200

ggcggctccg gcggctctgg cggcgtggac gatatccagc tgacccagtc ccctgcaatc     1260

atgagcgcct ccccaggaga gaaggtgaca atgacctgca gggccagctc ctctgtgagc     1320

tatatgaatt ggtaccagca gaagagcggc acatccccta agaggtggat ctatgacacc     1380

agcaaggtgg cctccggcgt gccatacaga ttctctggca gcggctccgg cacctcttat     1440

agcctgacaa tcagctccat ggaggccgag gatgccgcca cctattactg tcagcagtgg     1500

tctagcaatc ctctgacttt cggggctggg acaaaactgg aactgaagtg ataa           1554


<210>  4
<211>  516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, operably linked to IgE leader

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 
            20                  25                  30          


Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp 
        35                  40                  45              


Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile 
65                  70                  75                  80  


Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn 
                85                  90                  95      


Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln 
            100                 105                 110         


Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
145                 150                 155                 160 


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
                165                 170                 175     


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
            180                 185                 190         


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
        195                 200                 205             


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
    210                 215                 220                 


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
225                 230                 235                 240 


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
            260                 265                 270         


Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly 
        275                 280                 285             


Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg 
    290                 295                 300                 


Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
305                 310                 315                 320 


Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys 
                325                 330                 335     


Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala 
            340                 345                 350         


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
        355                 360                 365             


Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln 
    370                 375                 380                 


Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln 
                405                 410                 415     


Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr 
            420                 425                 430         


Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys 
        435                 440                 445             


Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala 
    450                 455                 460                 


Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
465                 470                 475                 480 


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
                485                 490                 495     


Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys 
            500                 505                 510         


Leu Glu Leu Lys 
        515     


<210>  5
<211>  1572
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, operably linked to IgE leader 
       and His tag

<400>  5
atggactgga cttggatcct gttcctggtc gccgccgcca cacgggtgca ttccgatatt       60

cagctgactc agagccctgc ttccctggcc gtgtccctgg gacagagggc aaccatctct      120

tgcaaggcct cccagtctgt ggactacgat ggcgactcct atctgaactg gtaccagcag      180

atcccaggcc agccccctaa gctgctgatc tatgacgcct ctaatctggt gagcggcatc      240

ccacccagat tcagcggctc cggctctggc acagacttca ccctgaacat ccacccagtg      300

gagaaggtgg acgcagcaac ctaccactgc cagcagtcca cagaggatcc ttggaccttc      360

ggcggcggca caaagctgga gatcaagggc ggcggcggca gcggcggcgg cggcagcggc      420

ggcggcggca gccaggtgca gctgcagcag agcggcgccg agctggtgcg gcccggcagc      480

tccgtgaaga tctcctgtaa ggcctctggc tatgcctttt ctagctactg gatgaattgg      540

gtgaagcagc gccccggaca gggcctggag tggatcggcc agatctggcc tggcgatggc      600

gacacaaact acaatggcaa gttcaagggc aaggccacac tgaccgccga cgagtcctct      660

agcaccgcct acatgcagct gtcctctctg gcctccgagg attctgccgt gtatttttgc      720

gccaggagag agacaacaac cgtgggcagg tactattacg ccatggacta ctggggccag      780

ggcacaaccg tgacagtgag cagcggcggc ggcggctctg atatcaagct gcagcagagc      840

ggagcagagc tggccagacc tggagccagc gtgaagatgt cctgtaagac ctctggctat      900

acattcaccc ggtacacaat gcactgggtg aagcagcgcc caggccaggg cctggaatgg      960

atcggctaca tcaaccccag ccggggctat accaactaca atcagaagtt taaggataag     1020

gccaccctga caaccgacaa gtctagctcc acagcctata tgcagctgtc tagcctgacc     1080

agcgaggact ccgccgtgta ttactgcgcc cgctattacg acgatcacta ttgtctggat     1140

tattggggac agggcacaac cctgacagtg tcctctgtgg agggcggctc tggcggcagc     1200

ggcggctccg gcggctctgg cggcgtggac gatatccagc tgacccagtc ccctgcaatc     1260

atgagcgcct ccccaggaga gaaggtgaca atgacctgca gggccagctc ctctgtgagc     1320

tatatgaatt ggtaccagca gaagagcggc acatccccta agaggtggat ctatgacacc     1380

agcaaggtgg cctccggcgt gccatacaga ttctctggca gcggctccgg cacctcttat     1440

agcctgacaa tcagctccat ggaggccgag gatgccgcca cctattactg tcagcagtgg     1500

tctagcaatc ctctgacttt cggggctggg acaaaactgg aactgaagca ccaccaccac     1560

caccattgat aa                                                         1572


<210>  6
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD19DBiTE, operably linked to IgE leader 
       and His tag

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 
            20                  25                  30          


Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp 
        35                  40                  45              


Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile 
65                  70                  75                  80  


Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn 
                85                  90                  95      


Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln 
            100                 105                 110         


Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
145                 150                 155                 160 


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
                165                 170                 175     


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
            180                 185                 190         


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
        195                 200                 205             


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
    210                 215                 220                 


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
225                 230                 235                 240 


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
            260                 265                 270         


Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly 
        275                 280                 285             


Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg 
    290                 295                 300                 


Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
305                 310                 315                 320 


Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys 
                325                 330                 335     


Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala 
            340                 345                 350         


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
        355                 360                 365             


Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln 
    370                 375                 380                 


Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln 
                405                 410                 415     


Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr 
            420                 425                 430         


Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys 
        435                 440                 445             


Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala 
    450                 455                 460                 


Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
465                 470                 475                 480 


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
                485                 490                 495     


Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys 
            500                 505                 510         


Leu Glu Leu Lys His His His His His His 
        515                 520         


<210>  7
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, without start, stop or IgE 
       leader

<400>  7
caggtgcagc tggtgcagtc aggagccgag gtcaagaagc ccggcgcctc cgtgaaggtg       60

tcttgcaagg cctccggcta ttctttccct gattactata tcaactgggt gcggcaggca      120

ccaggacagg gcctggagtg gatgggctgg atctatttcg ccagcggcaa ctccgagtac      180

aatcagaagt ttacaggccg ggtgaccatg acacgcgaca caagctcctc taccgcctac      240

atggagctga gctccctgcg ctccgaggat accgccgtgt atttctgcgc ctctctgtac      300

gactatgatt ggtactttga cgtgtggggc cagggcacaa tggtgaccgt gtctagcggc      360

ggcggcggca gcggcggcgg cggcagcggc ggcggcggct ccgatatcgt gatgacccag      420

acacctctgt ccctgtctgt gacacccggc gagcctgcca gcatctcctg taagtcctct      480

cagagcctgg tgcactccaa cggcaatacc tatctgcact ggtacctgca gaagcctggc      540

cagtccccac agctgctgat ctacaaggtg tctaaccggt tcagcggcgt gccagaccgc      600

ttttctggaa gcggatccgg agcagatttc acactgaaga tctctagggt ggaggccgag      660

gacgtgggcg tgtactattg cgccgagaca agccacgtgc cctggacatt tggccagggc      720

accaagctgg agatcaagag cggcggcggc ggctctgagg tgcagctggt ggagagcggc      780

ggcggcctgg tgcagcccgg cggctccctg aagctgtctt gtgccgccag cggcttcacc      840

ttcaacaagt atgccatgaa ttgggtgagg caggcacctg gcaagggcct ggagtgggtg      900

gccaggatca gaagcaagta caacaattat gccacctact atgccgactc cgtgaaggat      960

aggttcacaa tctccagaga cgattctaag aataccgcct acctgcagat gaacaatctg     1020

aagacagagg ataccgccgt gtactattgc gtgagacacg gcaactttgg caattcttac     1080

atcagctatt gggcctactg gggccagggc acactggtga ccgtgagcag cggcggcggc     1140

ggcagcggcg gcggcggcag cggcggcggc ggcagccaga cagtggtgac ccaggagcca     1200

agcctgaccg tgtcccctgg cggcaccgtg acactgacct gtggatctag cacaggagca     1260

gtgacctccg gaaactaccc taattgggtg cagcagaagc caggacaggc ccctaggggc     1320

ctgatcggcg gaacaaagtt cctggcccca ggcacccccg ccagattttc tggcagcctg     1380

ctgggcggca aggccgccct gaccctgtcc ggagtgcagc cagaggacga ggccgagtac     1440

tattgcgtgc tgtggtacag caatcgctgg gtcttcggag ggggaaccaa actgactgtc     1500

ctg                                                                   1503


<210>  8
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, without start or IgE leader

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Pro Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Tyr Phe Ala Ser Gly Asn Ser Glu Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Thr Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Leu Tyr Asp Tyr Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Thr Pro Leu Ser 
    130                 135                 140                 


Leu Ser Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Lys Ser Ser 
145                 150                 155                 160 


Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu 
                165                 170                 175     


Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn 
            180                 185                 190         


Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Ala 
        195                 200                 205             


Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val 
    210                 215                 220                 


Tyr Tyr Cys Ala Glu Thr Ser His Val Pro Trp Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val Gln Leu 
                245                 250                 255     


Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu 
            260                 265                 270         


Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp 
        275                 280                 285             


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg 
    290                 295                 300                 


Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp 
305                 310                 315                 320 


Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln 
                325                 330                 335     


Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg 
            340                 345                 350         


His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly 
        355                 360                 365             


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    370                 375                 380                 


Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro 
385                 390                 395                 400 


Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser 
                405                 410                 415     


Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln 
            420                 425                 430         


Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu 
        435                 440                 445             


Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys 
    450                 455                 460                 


Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr 
465                 470                 475                 480 


Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr 
                485                 490                 495     


Lys Leu Thr Val Leu 
            500     


<210>  9
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, operably linked to IgE leader

<400>  9
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtcaggtg       60

cagctggtgc agtcaggagc cgaggtcaag aagcccggcg cctccgtgaa ggtgtcttgc      120

aaggcctccg gctattcttt ccctgattac tatatcaact gggtgcggca ggcaccagga      180

cagggcctgg agtggatggg ctggatctat ttcgccagcg gcaactccga gtacaatcag      240

aagtttacag gccgggtgac catgacacgc gacacaagct cctctaccgc ctacatggag      300

ctgagctccc tgcgctccga ggataccgcc gtgtatttct gcgcctctct gtacgactat      360

gattggtact ttgacgtgtg gggccagggc acaatggtga ccgtgtctag cggcggcggc      420

ggcagcggcg gcggcggcag cggcggcggc ggctccgata tcgtgatgac ccagacacct      480

ctgtccctgt ctgtgacacc cggcgagcct gccagcatct cctgtaagtc ctctcagagc      540

ctggtgcact ccaacggcaa tacctatctg cactggtacc tgcagaagcc tggccagtcc      600

ccacagctgc tgatctacaa ggtgtctaac cggttcagcg gcgtgccaga ccgcttttct      660

ggaagcggat ccggagcaga tttcacactg aagatctcta gggtggaggc cgaggacgtg      720

ggcgtgtact attgcgccga gacaagccac gtgccctgga catttggcca gggcaccaag      780

ctggagatca agagcggcgg cggcggctct gaggtgcagc tggtggagag cggcggcggc      840

ctggtgcagc ccggcggctc cctgaagctg tcttgtgccg ccagcggctt caccttcaac      900

aagtatgcca tgaattgggt gaggcaggca cctggcaagg gcctggagtg ggtggccagg      960

atcagaagca agtacaacaa ttatgccacc tactatgccg actccgtgaa ggataggttc     1020

acaatctcca gagacgattc taagaatacc gcctacctgc agatgaacaa tctgaagaca     1080

gaggataccg ccgtgtacta ttgcgtgaga cacggcaact ttggcaattc ttacatcagc     1140

tattgggcct actggggcca gggcacactg gtgaccgtga gcagcggcgg cggcggcagc     1200

ggcggcggcg gcagcggcgg cggcggcagc cagacagtgg tgacccagga gccaagcctg     1260

accgtgtccc ctggcggcac cgtgacactg acctgtggat ctagcacagg agcagtgacc     1320

tccggaaact accctaattg ggtgcagcag aagccaggac aggcccctag gggcctgatc     1380

ggcggaacaa agttcctggc cccaggcacc cccgccagat tttctggcag cctgctgggc     1440

ggcaaggccg ccctgaccct gtccggagtg cagccagagg acgaggccga gtactattgc     1500

gtgctgtggt acagcaatcg ctgggtcttc ggagggggaa ccaaactgac tgtcctgtga     1560

taa                                                                   1563


<210>  10
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, operably linked to IgE leader

<400>  10

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Pro 
        35                  40                  45              


Asp Tyr Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
    50                  55                  60                  


Trp Met Gly Trp Ile Tyr Phe Ala Ser Gly Asn Ser Glu Tyr Asn Gln 
65                  70                  75                  80  


Lys Phe Thr Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ser Ser Thr 
                85                  90                  95      


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Phe Cys Ala Ser Leu Tyr Asp Tyr Asp Trp Tyr Phe Asp Val Trp Gly 
        115                 120                 125             


Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Thr Pro 
145                 150                 155                 160 


Leu Ser Leu Ser Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp 
            180                 185                 190         


Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys Val 
        195                 200                 205             


Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Ala Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val 
225                 230                 235                 240 


Gly Val Tyr Tyr Cys Ala Glu Thr Ser His Val Pro Trp Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val 
            260                 265                 270         


Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
        275                 280                 285             


Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met 
    290                 295                 300                 


Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg 
305                 310                 315                 320 


Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val 
                325                 330                 335     


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr 
            340                 345                 350         


Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys 
        355                 360                 365             


Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr 
    370                 375                 380                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln 
                405                 410                 415     


Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys 
            420                 425                 430         


Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val 
        435                 440                 445             


Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys 
    450                 455                 460                 


Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly 
465                 470                 475                 480 


Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala 
                485                 490                 495     


Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly 
            500                 505                 510         


Gly Thr Lys Leu Thr Val Leu 
        515                 


<210>  11
<211>  1587
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, operably linked to IgE leader 
       and His tag

<400>  11
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtcaggtg       60

cagctggtgc agtcaggagc cgaggtcaag aagcccggcg cctccgtgaa ggtgtcttgc      120

aaggcctccg gctattcttt ccctgattac tatatcaact gggtgcggca ggcaccagga      180

cagggcctgg agtggatggg ctggatctat ttcgccagcg gcaactccga gtacaatcag      240

aagtttacag gccgggtgac catgacacgc gacacaagct cctctaccgc ctacatggag      300

ctgagctccc tgcgctccga ggataccgcc gtgtatttct gcgcctctct gtacgactat      360

gattggtact ttgacgtgtg gggccagggc acaatggtga ccgtgtctag cggcggcggc      420

ggcagcggcg gcggcggcag cggcggcggc ggctccgata tcgtgatgac ccagacacct      480

ctgtccctgt ctgtgacacc cggcgagcct gccagcatct cctgtaagtc ctctcagagc      540

ctggtgcact ccaacggcaa tacctatctg cactggtacc tgcagaagcc tggccagtcc      600

ccacagctgc tgatctacaa ggtgtctaac cggttcagcg gcgtgccaga ccgcttttct      660

ggaagcggat ccggagcaga tttcacactg aagatctcta gggtggaggc cgaggacgtg      720

ggcgtgtact attgcgccga gacaagccac gtgccctgga catttggcca gggcaccaag      780

ctggagatca agagcggcgg cggcggctct gaggtgcagc tggtggagag cggcggcggc      840

ctggtgcagc ccggcggctc cctgaagctg tcttgtgccg ccagcggctt caccttcaac      900

aagtatgcca tgaattgggt gaggcaggca cctggcaagg gcctggagtg ggtggccagg      960

atcagaagca agtacaacaa ttatgccacc tactatgccg actccgtgaa ggataggttc     1020

acaatctcca gagacgattc taagaatacc gcctacctgc agatgaacaa tctgaagaca     1080

gaggataccg ccgtgtacta ttgcgtgaga cacggcaact ttggcaattc ttacatcagc     1140

tattgggcct actggggcca gggcacactg gtgaccgtga gcagcggcgg cggcggcagc     1200

ggcggcggcg gcagcggcgg cggcggcagc cagacagtgg tgacccagga gccaagcctg     1260

accgtgtccc ctggcggcac cgtgacactg acctgtggat ctagcacagg agcagtgacc     1320

tccggaaact accctaattg ggtgcagcag aagccaggac aggcccctag gggcctgatc     1380

ggcggaacaa agttcctggc cccaggcacc cccgccagat tttctggcag cctgctgggc     1440

ggcaaggccg ccctgaccct gtccggagtg cagccagagg acgaggccga gtactattgc     1500

gtgctgtggt acagcaatcg ctgggtcttc ggagggggaa ccaaactgac tgtcctgtga     1560

taacaccacc accaccacca ttgataa                                         1587


<210>  12
<211>  525
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, BCMADBiTE, operably linked to IgE leader 
       and His tag

<400>  12

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Pro 
        35                  40                  45              


Asp Tyr Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
    50                  55                  60                  


Trp Met Gly Trp Ile Tyr Phe Ala Ser Gly Asn Ser Glu Tyr Asn Gln 
65                  70                  75                  80  


Lys Phe Thr Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ser Ser Thr 
                85                  90                  95      


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Phe Cys Ala Ser Leu Tyr Asp Tyr Asp Trp Tyr Phe Asp Val Trp Gly 
        115                 120                 125             


Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Thr Pro 
145                 150                 155                 160 


Leu Ser Leu Ser Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp 
            180                 185                 190         


Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys Val 
        195                 200                 205             


Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Ala Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val 
225                 230                 235                 240 


Gly Val Tyr Tyr Cys Ala Glu Thr Ser His Val Pro Trp Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val 
            260                 265                 270         


Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
        275                 280                 285             


Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met 
    290                 295                 300                 


Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg 
305                 310                 315                 320 


Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val 
                325                 330                 335     


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr 
            340                 345                 350         


Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys 
        355                 360                 365             


Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr 
    370                 375                 380                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln 
                405                 410                 415     


Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys 
            420                 425                 430         


Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val 
        435                 440                 445             


Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys 
    450                 455                 460                 


Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly 
465                 470                 475                 480 


Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala 
                485                 490                 495     


Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly 
            500                 505                 510         


Gly Thr Lys Leu Thr Val Leu His His His His His His 
        515                 520                 525 


<210>  13
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33BiTE, without stop, start or IgE 
       leader

<400>  13
caggtgcagc tggtgcagtc aggggctgaa gtcaagaagc ccggcgagtc cgtgaaggtg       60

tcttgcaagg ccagcggcta taccttcaca aactacggca tgaattgggt gaagcaggcc      120

cctggccagt gtctggagtg gatgggctgg atcaacacct atacaggcga gccaacctac      180

gccgataagt ttcagggcag ggtgacaatg accacagaca cctccacatc taccgcctac      240

atggagatcc ggaatctggg cggcgacgat accgccgtgt actattgcgc ccgctggagc      300

tggtccgatg gctactacgt gtacttcgac tattggggcc agggcacctc cgtgacagtg      360

agcagcggcg gcggcggcag cggcggcggc ggcagcggcg gcggcggctc cgatatcgtg      420

atgacccagt cccccgactc tctgacagtg agcctgggcg agagaaccac aatcaactgt      480

aagtctagcc agagcgtgct ggattcctct accaacaaga acagcctggc atggtaccag      540

cagaagccag gacagccccc taagctgctg ctgagctggg catccaccag ggagtccggc      600

atccctgaca gattctctgg cagcggctcc ggcacagact ttacactgac catcgattcc      660

ccacagcccg aggactctgc cacctactat tgccagcagt ccgcccactt ccctatcacc      720

tttggctgtg gcacacggct ggagatcaag agcggcggcg gcggctctga ggtgcagctg      780

gtggagagcg gcggcggcct ggtgcagcca ggcggctccc tgaagctgtc ttgcgcagca      840

agcggcttca ccttcaacaa gtatgcaatg aattgggtgc gccaggcacc aggcaagggc      900

ctggagtggg tggccaggat cagatctaag tacaacaatt atgccaccta ctatgccgac      960

agcgtgaagg atcggttcac catctcccgc gacgattcta agaatacagc ctacctgcag     1020

atgaacaatc tgaagaccga ggatacagcc gtgtactatt gcgtgaggca cggcaacttt     1080

ggcaattctt acatcagcta ttgggcatac tggggacagg gcaccctggt cacagtgagc     1140

agcggcggcg gcggcagcgg cggcggcggc agcggcggcg gcggcagcca gaccgtggtg     1200

acacaggagc caagcctgac cgtgtcccct ggcggcacag tgaccctgac atgtggatct     1260

agcaccggag cagtgacaag cggaaactat cctaattggg tgcagcagaa acctggacag     1320

gcccctaggg gcctgatcgg cggaaccaag ttcctggccc ctggcacacc agccagattt     1380

tctggcagcc tgctgggcgg caaggccgcc ctgaccctgt ccggagtgca gccagaggac     1440

gaggccgagt actattgcgt gctgtggtac tctaatcgct gggtcttcgg gggagggaca     1500

aaactgactg tgctg                                                      1515


<210>  14
<211>  505
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33BiTE, without start or IgE leader

<400>  14

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Gly Gln Cys Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Ile Arg Asn Leu Gly Gly Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Ser Trp Ser Asp Gly Tyr Tyr Val Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser 
    130                 135                 140                 


Pro Asp Ser Leu Thr Val Ser Leu Gly Glu Arg Thr Thr Ile Asn Cys 
145                 150                 155                 160 


Lys Ser Ser Gln Ser Val Leu Asp Ser Ser Thr Asn Lys Asn Ser Leu 
                165                 170                 175     


Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Leu Ser 
            180                 185                 190         


Trp Ala Ser Thr Arg Glu Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asp Ser Pro Gln Pro Glu 
    210                 215                 220                 


Asp Ser Ala Thr Tyr Tyr Cys Gln Gln Ser Ala His Phe Pro Ile Thr 
225                 230                 235                 240 


Phe Gly Cys Gly Thr Arg Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
            260                 265                 270         


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr 
        275                 280                 285             


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
    290                 295                 300                 


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
305                 310                 315                 320 


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
                325                 330                 335     


Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr 
            340                 345                 350         


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp 
        355                 360                 365             


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val 
385                 390                 395                 400 


Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu 
                405                 410                 415     


Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn 
            420                 425                 430         


Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly 
        435                 440                 445             


Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu 
    450                 455                 460                 


Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp 
465                 470                 475                 480 


Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe 
                485                 490                 495     


Gly Gly Gly Thr Lys Leu Thr Val Leu 
            500                 505 


<210>  15
<211>  1575
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33DBiTE, operably linked to IgE leader

<400>  15
atggattgga catggattct gtttctggtc gccgccgcaa ctcgcgtcca ctcacaggtg       60

cagctggtgc agtcaggggc tgaagtcaag aagcccggcg agtccgtgaa ggtgtcttgc      120

aaggccagcg gctatacctt cacaaactac ggcatgaatt gggtgaagca ggcccctggc      180

cagtgtctgg agtggatggg ctggatcaac acctatacag gcgagccaac ctacgccgat      240

aagtttcagg gcagggtgac aatgaccaca gacacctcca catctaccgc ctacatggag      300

atccggaatc tgggcggcga cgataccgcc gtgtactatt gcgcccgctg gagctggtcc      360

gatggctact acgtgtactt cgactattgg ggccagggca cctccgtgac agtgagcagc      420

ggcggcggcg gcagcggcgg cggcggcagc ggcggcggcg gctccgatat cgtgatgacc      480

cagtcccccg actctctgac agtgagcctg ggcgagagaa ccacaatcaa ctgtaagtct      540

agccagagcg tgctggattc ctctaccaac aagaacagcc tggcatggta ccagcagaag      600

ccaggacagc cccctaagct gctgctgagc tgggcatcca ccagggagtc cggcatccct      660

gacagattct ctggcagcgg ctccggcaca gactttacac tgaccatcga ttccccacag      720

cccgaggact ctgccaccta ctattgccag cagtccgccc acttccctat cacctttggc      780

tgtggcacac ggctggagat caagagcggc ggcggcggct ctgaggtgca gctggtggag      840

agcggcggcg gcctggtgca gccaggcggc tccctgaagc tgtcttgcgc agcaagcggc      900

ttcaccttca acaagtatgc aatgaattgg gtgcgccagg caccaggcaa gggcctggag      960

tgggtggcca ggatcagatc taagtacaac aattatgcca cctactatgc cgacagcgtg     1020

aaggatcggt tcaccatctc ccgcgacgat tctaagaata cagcctacct gcagatgaac     1080

aatctgaaga ccgaggatac agccgtgtac tattgcgtga ggcacggcaa ctttggcaat     1140

tcttacatca gctattgggc atactgggga cagggcaccc tggtcacagt gagcagcggc     1200

ggcggcggca gcggcggcgg cggcagcggc ggcggcggca gccagaccgt ggtgacacag     1260

gagccaagcc tgaccgtgtc ccctggcggc acagtgaccc tgacatgtgg atctagcacc     1320

ggagcagtga caagcggaaa ctatcctaat tgggtgcagc agaaacctgg acaggcccct     1380

aggggcctga tcggcggaac caagttcctg gcccctggca caccagccag attttctggc     1440

agcctgctgg gcggcaaggc cgccctgacc ctgtccggag tgcagccaga ggacgaggcc     1500

gagtactatt gcgtgctgtg gtactctaat cgctgggtct tcgggggagg gacaaaactg     1560

actgtgctgt gataa                                                      1575


<210>  16
<211>  523
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33DBiTE, operably linked to IgE leader

<400>  16

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
        35                  40                  45              


Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly Gln Cys Leu Glu 
    50                  55                  60                  


Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp 
65                  70                  75                  80  


Lys Phe Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr 
                85                  90                  95      


Ala Tyr Met Glu Ile Arg Asn Leu Gly Gly Asp Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Trp Ser Trp Ser Asp Gly Tyr Tyr Val Tyr Phe Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr 
145                 150                 155                 160 


Gln Ser Pro Asp Ser Leu Thr Val Ser Leu Gly Glu Arg Thr Thr Ile 
                165                 170                 175     


Asn Cys Lys Ser Ser Gln Ser Val Leu Asp Ser Ser Thr Asn Lys Asn 
            180                 185                 190         


Ser Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        195                 200                 205             


Leu Ser Trp Ala Ser Thr Arg Glu Ser Gly Ile Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asp Ser Pro Gln 
225                 230                 235                 240 


Pro Glu Asp Ser Ala Thr Tyr Tyr Cys Gln Gln Ser Ala His Phe Pro 
                245                 250                 255     


Ile Thr Phe Gly Cys Gly Thr Arg Leu Glu Ile Lys Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
        275                 280                 285             


Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
    290                 295                 300                 


Lys Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
305                 310                 315                 320 


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
                325                 330                 335     


Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
            340                 345                 350         


Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala 
        355                 360                 365             


Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser 
    370                 375                 380                 


Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
                405                 410                 415     


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
            420                 425                 430         


Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr 
        435                 440                 445             


Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
    450                 455                 460                 


Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
465                 470                 475                 480 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
                485                 490                 495     


Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp 
            500                 505                 510         


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
        515                 520             


<210>  17
<211>  1593
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33DBiTE, operably linked to IgE leader 
       and His tag

<400>  17
atggattgga catggattct gtttctggtc gccgccgcaa ctcgcgtcca ctcacaggtg       60

cagctggtgc agtcaggggc tgaagtcaag aagcccggcg agtccgtgaa ggtgtcttgc      120

aaggccagcg gctatacctt cacaaactac ggcatgaatt gggtgaagca ggcccctggc      180

cagtgtctgg agtggatggg ctggatcaac acctatacag gcgagccaac ctacgccgat      240

aagtttcagg gcagggtgac aatgaccaca gacacctcca catctaccgc ctacatggag      300

atccggaatc tgggcggcga cgataccgcc gtgtactatt gcgcccgctg gagctggtcc      360

gatggctact acgtgtactt cgactattgg ggccagggca cctccgtgac agtgagcagc      420

ggcggcggcg gcagcggcgg cggcggcagc ggcggcggcg gctccgatat cgtgatgacc      480

cagtcccccg actctctgac agtgagcctg ggcgagagaa ccacaatcaa ctgtaagtct      540

agccagagcg tgctggattc ctctaccaac aagaacagcc tggcatggta ccagcagaag      600

ccaggacagc cccctaagct gctgctgagc tgggcatcca ccagggagtc cggcatccct      660

gacagattct ctggcagcgg ctccggcaca gactttacac tgaccatcga ttccccacag      720

cccgaggact ctgccaccta ctattgccag cagtccgccc acttccctat cacctttggc      780

tgtggcacac ggctggagat caagagcggc ggcggcggct ctgaggtgca gctggtggag      840

agcggcggcg gcctggtgca gccaggcggc tccctgaagc tgtcttgcgc agcaagcggc      900

ttcaccttca acaagtatgc aatgaattgg gtgcgccagg caccaggcaa gggcctggag      960

tgggtggcca ggatcagatc taagtacaac aattatgcca cctactatgc cgacagcgtg     1020

aaggatcggt tcaccatctc ccgcgacgat tctaagaata cagcctacct gcagatgaac     1080

aatctgaaga ccgaggatac agccgtgtac tattgcgtga ggcacggcaa ctttggcaat     1140

tcttacatca gctattgggc atactgggga cagggcaccc tggtcacagt gagcagcggc     1200

ggcggcggca gcggcggcgg cggcagcggc ggcggcggca gccagaccgt ggtgacacag     1260

gagccaagcc tgaccgtgtc ccctggcggc acagtgaccc tgacatgtgg atctagcacc     1320

ggagcagtga caagcggaaa ctatcctaat tgggtgcagc agaaacctgg acaggcccct     1380

aggggcctga tcggcggaac caagttcctg gcccctggca caccagccag attttctggc     1440

agcctgctgg gcggcaaggc cgccctgacc ctgtccggag tgcagccaga ggacgaggcc     1500

gagtactatt gcgtgctgtg gtactctaat cgctgggtct tcgggggagg gacaaaactg     1560

actgtgctgc atcaccacca ccaccattga taa                                  1593


<210>  18
<211>  529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD33DBiTE, operably linked to IgE leader 
       and His tag

<400>  18

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
        35                  40                  45              


Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly Gln Cys Leu Glu 
    50                  55                  60                  


Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp 
65                  70                  75                  80  


Lys Phe Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr 
                85                  90                  95      


Ala Tyr Met Glu Ile Arg Asn Leu Gly Gly Asp Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Trp Ser Trp Ser Asp Gly Tyr Tyr Val Tyr Phe Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr 
145                 150                 155                 160 


Gln Ser Pro Asp Ser Leu Thr Val Ser Leu Gly Glu Arg Thr Thr Ile 
                165                 170                 175     


Asn Cys Lys Ser Ser Gln Ser Val Leu Asp Ser Ser Thr Asn Lys Asn 
            180                 185                 190         


Ser Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        195                 200                 205             


Leu Ser Trp Ala Ser Thr Arg Glu Ser Gly Ile Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asp Ser Pro Gln 
225                 230                 235                 240 


Pro Glu Asp Ser Ala Thr Tyr Tyr Cys Gln Gln Ser Ala His Phe Pro 
                245                 250                 255     


Ile Thr Phe Gly Cys Gly Thr Arg Leu Glu Ile Lys Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
        275                 280                 285             


Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
    290                 295                 300                 


Lys Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
305                 310                 315                 320 


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
                325                 330                 335     


Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
            340                 345                 350         


Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala 
        355                 360                 365             


Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser 
    370                 375                 380                 


Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
                405                 410                 415     


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
            420                 425                 430         


Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr 
        435                 440                 445             


Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
    450                 455                 460                 


Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
465                 470                 475                 480 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
                485                 490                 495     


Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp 
            500                 505                 510         


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu His His His His His 
        515                 520                 525             


His 
    


<210>  19
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPBiTE, without start, stop or IgE 
       leader

<400>  19
gaagtgcagc tgctggaaag tggcgggggc ctggtccagc ctggcgggtc cctgagactg       60

agttgtgctg cctccgggtt tacatttagc tcctacgcaa tgtcctgggt gaggcaggca      120

ccaggcaagg gcctggagtg ggtgtctgcc atctccggct ctggcggctc tacctactat      180

gccgacagcg tgaagggcag gttcaccatc agcagagata actccaagaa tacactgtac      240

ctgcagatga acagcctgag ggccgaggac acagccgtgt actattgcgc caagggctgg      300

ctgggcaatt ttgattattg gggccagggc accctggtga cagtgtctag cggcggcggc      360

ggcagcggcg gcggcggcag cggcggcggc ggctccgaga tcgtgctgac ccagagccca      420

ggcacactga gcctgtcccc aggagagagg gccaccctgt cctgtcgcgc ctctcagagc      480

gtgtcccggt cttacctggc ctggtatcag cagaagccag gacaggcccc tcgcctgctg      540

atcatcggag catctaccag ggcaacaggc atccctgaca gattcagcgg ctccggctct      600

ggcacagact tcaccctgac aatctccaga ctggagccag aggacttcgc cgtgtactat      660

tgtcagcagg ggcaggtcat tccacccaca ttcgggcagg gcactaaagt cgagatcaaa      720

ggcggcggcg gctctgaggt gcagctggtg gagagcggcg gcggcctggt gcagcccggc      780

ggctccctga agctgtcttg tgccgccagc ggcttcacct tcaacaagta tgccatgaat      840

tgggtgaggc aggcacctgg caagggcctg gagtgggtgg ccaggatcag aagcaagtac      900

aacaattatg ccacctacta tgccgactcc gtgaaggata ggttcacaat ctccagagac      960

gattctaaga ataccgccta cctgcagatg aacaatctga agacagagga taccgccgtg     1020

tactattgcg tgagacacgg caactttggc aattcttaca tcagctattg ggcctactgg     1080

ggccagggca cactggtgac cgtgagcagc ggcggcggcg gcagcggcgg cggcggcagc     1140

ggcggcggcg gcagccagac agtggtgacc caggagccaa gcctgaccgt gtcccctggc     1200

ggcaccgtga cactgacctg tggatctagc acaggagcag tgacctccgg aaactaccct     1260

aattgggtgc agcagaagcc aggacaggcc cctaggggcc tgatcggcgg aacaaagttc     1320

ctggccccag gcacccccgc cagattttct ggcagcctgc tgggcggcaa ggccgccctg     1380

accctgtccg gagtgcagcc agaggacgag gccgagtact attgcgtgct gtggtacagc     1440

aatcgctggg tcttcggagg gggaaccaaa ctgactgtcc tg                        1482


<210>  20
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPBiTE, without start or IgE leader

<400>  20

Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
1               5                   10                  15      


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Ala 
            20                  25                  30          


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
        35                  40                  45              


Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Gly Trp Leu Gly Asn Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu 
    130                 135                 140                 


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val 
145                 150                 155                 160 


Ser Arg Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
                165                 170                 175     


Arg Leu Leu Ile Ile Gly Ala Ser Thr Arg Ala Thr Gly Ile Pro Asp 
            180                 185                 190         


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        195                 200                 205             


Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Gly Gln 
    210                 215                 220                 


Val Ile Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
                245                 250                 255     


Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr 
            260                 265                 270         


Phe Asn Lys Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly 
        275                 280                 285             


Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr 
    290                 295                 300                 


Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp 
305                 310                 315                 320 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp 
                325                 330                 335     


Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr 
            340                 345                 350         


Ile Ser Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        355                 360                 365             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 


Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
385                 390                 395                 400 


Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly 
                405                 410                 415     


Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
            420                 425                 430         


Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe 
        435                 440                 445             


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val 
    450                 455                 460                 


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn 
465                 470                 475                 480 


Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
                485                 490             


<210>  21
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPDBiTE, operably linked to IgE leader

<400>  21
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtgaagtg       60

cagctgctgg aaagtggcgg gggcctggtc cagcctggcg ggtccctgag actgagttgt      120

gctgcctccg ggtttacatt tagctcctac gcaatgtcct gggtgaggca ggcaccaggc      180

aagggcctgg agtgggtgtc tgccatctcc ggctctggcg gctctaccta ctatgccgac      240

agcgtgaagg gcaggttcac catcagcaga gataactcca agaatacact gtacctgcag      300

atgaacagcc tgagggccga ggacacagcc gtgtactatt gcgccaaggg ctggctgggc      360

aattttgatt attggggcca gggcaccctg gtgacagtgt ctagcggcgg cggcggcagc      420

ggcggcggcg gcagcggcgg cggcggctcc gagatcgtgc tgacccagag cccaggcaca      480

ctgagcctgt ccccaggaga gagggccacc ctgtcctgtc gcgcctctca gagcgtgtcc      540

cggtcttacc tggcctggta tcagcagaag ccaggacagg cccctcgcct gctgatcatc      600

ggagcatcta ccagggcaac aggcatccct gacagattca gcggctccgg ctctggcaca      660

gacttcaccc tgacaatctc cagactggag ccagaggact tcgccgtgta ctattgtcag      720

caggggcagg tcattccacc cacattcggg cagggcacta aagtcgagat caaaggcggc      780

ggcggctctg aggtgcagct ggtggagagc ggcggcggcc tggtgcagcc cggcggctcc      840

ctgaagctgt cttgtgccgc cagcggcttc accttcaaca agtatgccat gaattgggtg      900

aggcaggcac ctggcaaggg cctggagtgg gtggccagga tcagaagcaa gtacaacaat      960

tatgccacct actatgccga ctccgtgaag gataggttca caatctccag agacgattct     1020

aagaataccg cctacctgca gatgaacaat ctgaagacag aggataccgc cgtgtactat     1080

tgcgtgagac acggcaactt tggcaattct tacatcagct attgggccta ctggggccag     1140

ggcacactgg tgaccgtgag cagcggcggc ggcggcagcg gcggcggcgg cagcggcggc     1200

ggcggcagcc agacagtggt gacccaggag ccaagcctga ccgtgtcccc tggcggcacc     1260

gtgacactga cctgtggatc tagcacagga gcagtgacct ccggaaacta ccctaattgg     1320

gtgcagcaga agccaggaca ggcccctagg ggcctgatcg gcggaacaaa gttcctggcc     1380

ccaggcaccc ccgccagatt ttctggcagc ctgctgggcg gcaaggccgc cctgaccctg     1440

tccggagtgc agccagagga cgaggccgag tactattgcg tgctgtggta cagcaatcgc     1500

tgggtcttcg gagggggaac caaactgact gtcctgtgat aa                        1542


<210>  22
<211>  512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPDBiTE, operably linked to IgE leader

<400>  22

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
        35                  40                  45              


Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Lys Gly Trp Leu Gly Asn Phe Asp Tyr Trp Gly Gln Gly 
        115                 120                 125             


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr 
145                 150                 155                 160 


Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser 
                165                 170                 175     


Gln Ser Val Ser Arg Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
            180                 185                 190         


Gln Ala Pro Arg Leu Leu Ile Ile Gly Ala Ser Thr Arg Ala Thr Gly 
        195                 200                 205             


Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gln Gly Gln Val Ile Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
                245                 250                 255     


Ile Lys Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            260                 265                 270         


Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser 
        275                 280                 285             


Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val Arg Gln Ala Pro 
    290                 295                 300                 


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn 
305                 310                 315                 320 


Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser 
                325                 330                 335     


Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys 
            340                 345                 350         


Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly 
        355                 360                 365             


Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val 
    370                 375                 380                 


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser 
                405                 410                 415     


Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val 
            420                 425                 430         


Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala 
        435                 440                 445             


Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro 
    450                 455                 460                 


Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu 
465                 470                 475                 480 


Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp 
                485                 490                 495     


Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            500                 505                 510         


<210>  23
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPDBiTE, operably linked to IgE leader 
       and His tag

<400>  23
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtgaagtg       60

cagctgctgg aaagtggcgg gggcctggtc cagcctggcg ggtccctgag actgagttgt      120

gctgcctccg ggtttacatt tagctcctac gcaatgtcct gggtgaggca ggcaccaggc      180

aagggcctgg agtgggtgtc tgccatctcc ggctctggcg gctctaccta ctatgccgac      240

agcgtgaagg gcaggttcac catcagcaga gataactcca agaatacact gtacctgcag      300

atgaacagcc tgagggccga ggacacagcc gtgtactatt gcgccaaggg ctggctgggc      360

aattttgatt attggggcca gggcaccctg gtgacagtgt ctagcggcgg cggcggcagc      420

ggcggcggcg gcagcggcgg cggcggctcc gagatcgtgc tgacccagag cccaggcaca      480

ctgagcctgt ccccaggaga gagggccacc ctgtcctgtc gcgcctctca gagcgtgtcc      540

cggtcttacc tggcctggta tcagcagaag ccaggacagg cccctcgcct gctgatcatc      600

ggagcatcta ccagggcaac aggcatccct gacagattca gcggctccgg ctctggcaca      660

gacttcaccc tgacaatctc cagactggag ccagaggact tcgccgtgta ctattgtcag      720

caggggcagg tcattccacc cacattcggg cagggcacta aagtcgagat caaaggcggc      780

ggcggctctg aggtgcagct ggtggagagc ggcggcggcc tggtgcagcc cggcggctcc      840

ctgaagctgt cttgtgccgc cagcggcttc accttcaaca agtatgccat gaattgggtg      900

aggcaggcac ctggcaaggg cctggagtgg gtggccagga tcagaagcaa gtacaacaat      960

tatgccacct actatgccga ctccgtgaag gataggttca caatctccag agacgattct     1020

aagaataccg cctacctgca gatgaacaat ctgaagacag aggataccgc cgtgtactat     1080

tgcgtgagac acggcaactt tggcaattct tacatcagct attgggccta ctggggccag     1140

ggcacactgg tgaccgtgag cagcggcggc ggcggcagcg gcggcggcgg cagcggcggc     1200

ggcggcagcc agacagtggt gacccaggag ccaagcctga ccgtgtcccc tggcggcacc     1260

gtgacactga cctgtggatc tagcacagga gcagtgacct ccggaaacta ccctaattgg     1320

gtgcagcaga agccaggaca ggcccctagg ggcctgatcg gcggaacaaa gttcctggcc     1380

ccaggcaccc ccgccagatt ttctggcagc ctgctgggcg gcaaggccgc cctgaccctg     1440

tccggagtgc agccagagga cgaggccgag tactattgcg tgctgtggta cagcaatcgc     1500

tgggtcttcg gagggggaac caaactgact gtcctgcacc accaccacca ccattgataa     1560


<210>  24
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FAPDBiTE, operably linked to IgE leader 
       and His tag

<400>  24

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
        35                  40                  45              


Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Lys Gly Trp Leu Gly Asn Phe Asp Tyr Trp Gly Gln Gly 
        115                 120                 125             


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr 
145                 150                 155                 160 


Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser 
                165                 170                 175     


Gln Ser Val Ser Arg Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
            180                 185                 190         


Gln Ala Pro Arg Leu Leu Ile Ile Gly Ala Ser Thr Arg Ala Thr Gly 
        195                 200                 205             


Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gln Gly Gln Val Ile Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
                245                 250                 255     


Ile Lys Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            260                 265                 270         


Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser 
        275                 280                 285             


Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val Arg Gln Ala Pro 
    290                 295                 300                 


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn 
305                 310                 315                 320 


Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser 
                325                 330                 335     


Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys 
            340                 345                 350         


Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly 
        355                 360                 365             


Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val 
    370                 375                 380                 


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser 
                405                 410                 415     


Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val 
            420                 425                 430         


Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala 
        435                 440                 445             


Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro 
    450                 455                 460                 


Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu 
465                 470                 475                 480 


Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp 
                485                 490                 495     


Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            500                 505                 510         


His His His His His His 
        515             


<210>  25
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, without start or stop codons 
       or IgE leader

<400>  25
tacaccaggg acctggtgta taaggatccc gccagaccta agatccagaa gacctgcaca       60

ttcggcggcg gcggctctga ggtgcagctg gtggagagcg gcggcggcct ggtgcagccc      120

ggcggctccc tgaagctgtc ttgtgccgcc agcggcttca ccttcaacaa gtatgccatg      180

aattgggtga ggcaggcacc tggcaagggc ctggagtggg tggccaggat cagaagcaag      240

tacaacaatt atgccaccta ctatgccgac tccgtgaagg ataggttcac aatctccaga      300

gacgattcta agaataccgc ctacctgcag atgaacaatc tgaagacaga ggataccgcc      360

gtgtactatt gcgtgagaca cggcaacttt ggcaattctt acatcagcta ttgggcctac      420

tggggccagg gcacactggt gaccgtgagc agcggcggcg gcggcagcgg cggcggcggc      480

agcggcggcg gcggcagcca gacagtggtg acccaggagc caagcctgac cgtgtcccct      540

ggcggcaccg tgacactgac ctgtggatct agcacaggag cagtgacctc cggaaactac      600

cctaattggg tgcagcagaa gccaggacag gcccctaggg gcctgatcgg cggaacaaag      660

ttcctggccc caggcacccc cgccagattt tctggcagcc tgctgggcgg caaggccgcc      720

ctgaccctgt ccggagtgca gccagaggac gaggccgagt actattgcgt gctgtggtac      780

agcaatcgct gggtcttcgg agggggaacc aaactgactg tcctg                      825


<210>  26
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, without start or IgE leader 
       sequence

<400>  26

Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln 
1               5                   10                  15      


Lys Thr Cys Thr Phe Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu 
            20                  25                  30          


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys 
        35                  40                  45              


Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val Arg 
    50                  55                  60                  


Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys 
65                  70                  75                  80  


Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe 
                85                  90                  95      


Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn 
            100                 105                 110         


Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly 
        115                 120                 125             


Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln Gly 
    130                 135                 140                 


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu 
                165                 170                 175     


Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr 
            180                 185                 190         


Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro 
        195                 200                 205             


Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro 
    210                 215                 220                 


Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala 
225                 230                 235                 240 


Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys 
                245                 250                 255     


Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu 
            260                 265                 270         


Thr Val Leu 
        275 


<210>  27
<211>  885
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, operably linked to IgE leader

<400>  27
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagttacacc       60

agggacctgg tgtataagga tcccgccaga cctaagatcc agaagacctg cacattcggc      120

ggcggcggct ctgaggtgca gctggtggag agcggcggcg gcctggtgca gcccggcggc      180

tccctgaagc tgtcttgtgc cgccagcggc ttcaccttca acaagtatgc catgaattgg      240

gtgaggcagg cacctggcaa gggcctggag tgggtggcca ggatcagaag caagtacaac      300

aattatgcca cctactatgc cgactccgtg aaggataggt tcacaatctc cagagacgat      360

tctaagaata ccgcctacct gcagatgaac aatctgaaga cagaggatac cgccgtgtac      420

tattgcgtga gacacggcaa ctttggcaat tcttacatca gctattgggc ctactggggc      480

cagggcacac tggtgaccgt gagcagcggc ggcggcggca gcggcggcgg cggcagcggc      540

ggcggcggca gccagacagt ggtgacccag gagccaagcc tgaccgtgtc ccctggcggc      600

accgtgacac tgacctgtgg atctagcaca ggagcagtga cctccggaaa ctaccctaat      660

tgggtgcagc agaagccagg acaggcccct aggggcctga tcggcggaac aaagttcctg      720

gccccaggca cccccgccag attttctggc agcctgctgg gcggcaaggc cgccctgacc      780

ctgtccggag tgcagccaga ggacgaggcc gagtactatt gcgtgctgtg gtacagcaat      840

cgctgggtct tcggaggggg aaccaaactg actgtcctgt gataa                      885


<210>  28
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, operably linked to IgE leader

<400>  28

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys 
            20                  25                  30          


Ile Gln Lys Thr Cys Thr Phe Gly Gly Gly Gly Ser Glu Val Gln Leu 
        35                  40                  45              


Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu 
    50                  55                  60                  


Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp 
65                  70                  75                  80  


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg 
                85                  90                  95      


Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp 
            100                 105                 110         


Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln 
        115                 120                 125             


Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg 
    130                 135                 140                 


His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly 
145                 150                 155                 160 


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
                165                 170                 175     


Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro 
            180                 185                 190         


Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser 
        195                 200                 205             


Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln 
    210                 215                 220                 


Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu 
225                 230                 235                 240 


Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys 
                245                 250                 255     


Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr 
            260                 265                 270         


Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr 
        275                 280                 285             


Lys Leu Thr Val Leu 
    290             


<210>  29
<211>  903
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, operably linked to IgE leader 
       and His tag

<400>  29
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagttacacc       60

agggacctgg tgtataagga tcccgccaga cctaagatcc agaagacctg cacattcggc      120

ggcggcggct ctgaggtgca gctggtggag agcggcggcg gcctggtgca gcccggcggc      180

tccctgaagc tgtcttgtgc cgccagcggc ttcaccttca acaagtatgc catgaattgg      240

gtgaggcagg cacctggcaa gggcctggag tgggtggcca ggatcagaag caagtacaac      300

aattatgcca cctactatgc cgactccgtg aaggataggt tcacaatctc cagagacgat      360

tctaagaata ccgcctacct gcagatgaac aatctgaaga cagaggatac cgccgtgtac      420

tattgcgtga gacacggcaa ctttggcaat tcttacatca gctattgggc ctactggggc      480

cagggcacac tggtgaccgt gagcagcggc ggcggcggca gcggcggcgg cggcagcggc      540

ggcggcggca gccagacagt ggtgacccag gagccaagcc tgaccgtgtc ccctggcggc      600

accgtgacac tgacctgtgg atctagcaca ggagcagtga cctccggaaa ctaccctaat      660

tgggtgcagc agaagccagg acaggcccct aggggcctga tcggcggaac aaagttcctg      720

gccccaggca cccccgccag attttctggc agcctgctgg gcggcaaggc cgccctgacc      780

ctgtccggag tgcagccaga ggacgaggcc gagtactatt gcgtgctgtg gtacagcaat      840

cgctgggtct tcggaggggg aaccaaactg actgtcctgc accaccacca ccaccattga      900

taa                                                                    903


<210>  30
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, FSHRDBiTE, operably linked to IgE leader 
       and His tag

<400>  30

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys 
            20                  25                  30          


Ile Gln Lys Thr Cys Thr Phe Gly Gly Gly Gly Ser Glu Val Gln Leu 
        35                  40                  45              


Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu 
    50                  55                  60                  


Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp 
65                  70                  75                  80  


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg 
                85                  90                  95      


Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp 
            100                 105                 110         


Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln 
        115                 120                 125             


Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg 
    130                 135                 140                 


His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly 
145                 150                 155                 160 


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
                165                 170                 175     


Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro 
            180                 185                 190         


Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser 
        195                 200                 205             


Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln 
    210                 215                 220                 


Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu 
225                 230                 235                 240 


Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys 
                245                 250                 255     


Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr 
            260                 265                 270         


Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr 
        275                 280                 285             


Lys Leu Thr Val Leu His His His His His His 
    290                 295                 


<210>  31
<211>  1500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRBiTE, without start, stop or IgE 
       leader

<400>  31
gagatccagc tggtgcagag cggagccgag gtcaagaagc ctggggagag cctgagaatt       60

tcctgtaagg ggagcgggtt caacattgag gactactata tccactgggt gaggcagatg      120

ccaggcaagg gcctggagtg gatgggaaga atcgacccag agaacgatga gacaaagtac      180

ggccccatct tccagggcca cgtgacaatc agcgccgaca cctccatcaa tacagtgtat      240

ctgcagtgga gctccctgaa ggcctccgat accgccatgt actattgcgc ctttaggggc      300

ggcgtgtact ggggacaggg aaccacagtg acagtgtcta gcggcggcgg cggcagcggc      360

ggcggcggca gcggcggcgg cggcagcggc ggcggcggct ccgacgtggt catgacccag      420

tctcctgata gcctggccgt gtctctggga gagagggcaa caatcaactg taagtcctct      480

cagtctctgc tggacagcga tggcaagacc tatctgaatt ggctgcagca gaagcctggc      540

cagcccccta agcggctgat ctccctggtg tctaagctgg acagcggcgt gccagatcgc      600

ttctctggca gcggctccgg cacagacttt accctgacaa tcagctccct gcaggccgag      660

gatgtggccg tgtactattg ttggcagggg actcattttc cagggacatt cggaggaggg      720

acaaaggtcg agatcaaggg cggcggcggc tctgaggtgc agctggtgga gagcggcggc      780

ggcctggtgc agcccggcgg ctccctgaag ctgtcttgtg ccgccagcgg cttcaccttc      840

aacaagtatg ccatgaattg ggtgaggcag gcacctggca agggcctgga gtgggtggcc      900

aggatcagaa gcaagtacaa caattatgcc acctactatg ccgactccgt gaaggatagg      960

ttcacaatct ccagagacga ttctaagaat accgcctacc tgcagatgaa caatctgaag     1020

acagaggata ccgccgtgta ctattgcgtg agacacggca actttggcaa ttcttacatc     1080

agctattggg cctactgggg ccagggcaca ctggtgaccg tgagcagcgg cggcggcggc     1140

agcggcggcg gcggcagcgg cggcggcggc agccagacag tggtgaccca ggagccaagc     1200

ctgaccgtgt cccctggcgg caccgtgaca ctgacctgtg gatctagcac aggagcagtg     1260

acctccggaa actaccctaa ttgggtgcag cagaagccag gacaggcccc taggggcctg     1320

atcggcggaa caaagttcct ggccccaggc acccccgcca gattttctgg cagcctgctg     1380

ggcggcaagg ccgccctgac cctgtccgga gtgcagccag aggacgaggc cgagtactat     1440

tgcgtgctgt ggtacagcaa tcgctgggtc ttcggagggg gaaccaaact gactgtcctg     1500


<210>  32
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRBiTE, without start or IgE leader

<400>  32

Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn Ile Glu Asp Tyr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr Gly Pro Ile Phe 
    50                  55                  60                  


Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr Thr Val Thr Val 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Asp Ser 
    130                 135                 140                 


Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 
145                 150                 155                 160 


Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Gln 
                165                 170                 175     


Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser Leu Val Ser Lys 
            180                 185                 190         


Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr Phe Gly Gly Gly 
225                 230                 235                 240 


Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
                245                 250                 255     


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser 
            260                 265                 270         


Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val 
        275                 280                 285             


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser 
    290                 295                 300                 


Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg 
305                 310                 315                 320 


Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met 
                325                 330                 335     


Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His 
            340                 345                 350         


Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln 
        355                 360                 365             


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser 
385                 390                 395                 400 


Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser 
                405                 410                 415     


Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys 
            420                 425                 430         


Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala 
        435                 440                 445             


Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala 
    450                 455                 460                 


Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr 
465                 470                 475                 480 


Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys 
                485                 490                 495     


Leu Thr Val Leu 
            500 


<210>  33
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRDBiTE, operably linked to IgE leader

<400>  33
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtgagatc       60

cagctggtgc agagcggagc cgaggtcaag aagcctgggg agagcctgag aatttcctgt      120

aaggggagcg ggttcaacat tgaggactac tatatccact gggtgaggca gatgccaggc      180

aagggcctgg agtggatggg aagaatcgac ccagagaacg atgagacaaa gtacggcccc      240

atcttccagg gccacgtgac aatcagcgcc gacacctcca tcaatacagt gtatctgcag      300

tggagctccc tgaaggcctc cgataccgcc atgtactatt gcgcctttag gggcggcgtg      360

tactggggac agggaaccac agtgacagtg tctagcggcg gcggcggcag cggcggcggc      420

ggcagcggcg gcggcggcag cggcggcggc ggctccgacg tggtcatgac ccagtctcct      480

gatagcctgg ccgtgtctct gggagagagg gcaacaatca actgtaagtc ctctcagtct      540

ctgctggaca gcgatggcaa gacctatctg aattggctgc agcagaagcc tggccagccc      600

cctaagcggc tgatctccct ggtgtctaag ctggacagcg gcgtgccaga tcgcttctct      660

ggcagcggct ccggcacaga ctttaccctg acaatcagct ccctgcaggc cgaggatgtg      720

gccgtgtact attgttggca ggggactcat tttccaggga cattcggagg agggacaaag      780

gtcgagatca agggcggcgg cggctctgag gtgcagctgg tggagagcgg cggcggcctg      840

gtgcagcccg gcggctccct gaagctgtct tgtgccgcca gcggcttcac cttcaacaag      900

tatgccatga attgggtgag gcaggcacct ggcaagggcc tggagtgggt ggccaggatc      960

agaagcaagt acaacaatta tgccacctac tatgccgact ccgtgaagga taggttcaca     1020

atctccagag acgattctaa gaataccgcc tacctgcaga tgaacaatct gaagacagag     1080

gataccgccg tgtactattg cgtgagacac ggcaactttg gcaattctta catcagctat     1140

tgggcctact ggggccaggg cacactggtg accgtgagca gcggcggcgg cggcagcggc     1200

ggcggcggca gcggcggcgg cggcagccag acagtggtga cccaggagcc aagcctgacc     1260

gtgtcccctg gcggcaccgt gacactgacc tgtggatcta gcacaggagc agtgacctcc     1320

ggaaactacc ctaattgggt gcagcagaag ccaggacagg cccctagggg cctgatcggc     1380

ggaacaaagt tcctggcccc aggcaccccc gccagatttt ctggcagcct gctgggcggc     1440

aaggccgccc tgaccctgtc cggagtgcag ccagaggacg aggccgagta ctattgcgtg     1500

ctgtggtaca gcaatcgctg ggtcttcgga gggggaacca aactgactgt cctgtgataa     1560


<210>  34
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRDBiTE, operably linked to IgE leader

<400>  34

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn Ile Glu 
        35                  40                  45              


Asp Tyr Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Met Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr Gly Pro 
65                  70                  75                  80  


Ile Phe Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile Asn Thr 
                85                  90                  95      


Val Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr 
            100                 105                 110         


Tyr Cys Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr Thr Val 
        115                 120                 125             


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro 
145                 150                 155                 160 


Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp 
            180                 185                 190         


Leu Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser Leu Val 
        195                 200                 205             


Ser Lys Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val 
225                 230                 235                 240 


Ala Val Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile 
305                 310                 315                 320 


Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys 
                325                 330                 335     


Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu 
            340                 345                 350         


Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val 
        355                 360                 365             


Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp 
    370                 375                 380                 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu 
                405                 410                 415     


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly 
            420                 425                 430         


Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln 
        435                 440                 445             


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe 
    450                 455                 460                 


Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
465                 470                 475                 480 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
                485                 490                 495     


Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly 
            500                 505                 510         


Thr Lys Leu Thr Val Leu 
        515             


<210>  35
<211>  1578
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRDBiTE, operably linked to IgE leader 
       and His tag

<400>  35
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtgagatc       60

cagctggtgc agagcggagc cgaggtcaag aagcctgggg agagcctgag aatttcctgt      120

aaggggagcg ggttcaacat tgaggactac tatatccact gggtgaggca gatgccaggc      180

aagggcctgg agtggatggg aagaatcgac ccagagaacg atgagacaaa gtacggcccc      240

atcttccagg gccacgtgac aatcagcgcc gacacctcca tcaatacagt gtatctgcag      300

tggagctccc tgaaggcctc cgataccgcc atgtactatt gcgcctttag gggcggcgtg      360

tactggggac agggaaccac agtgacagtg tctagcggcg gcggcggcag cggcggcggc      420

ggcagcggcg gcggcggcag cggcggcggc ggctccgacg tggtcatgac ccagtctcct      480

gatagcctgg ccgtgtctct gggagagagg gcaacaatca actgtaagtc ctctcagtct      540

ctgctggaca gcgatggcaa gacctatctg aattggctgc agcagaagcc tggccagccc      600

cctaagcggc tgatctccct ggtgtctaag ctggacagcg gcgtgccaga tcgcttctct      660

ggcagcggct ccggcacaga ctttaccctg acaatcagct ccctgcaggc cgaggatgtg      720

gccgtgtact attgttggca ggggactcat tttccaggga cattcggagg agggacaaag      780

gtcgagatca agggcggcgg cggctctgag gtgcagctgg tggagagcgg cggcggcctg      840

gtgcagcccg gcggctccct gaagctgtct tgtgccgcca gcggcttcac cttcaacaag      900

tatgccatga attgggtgag gcaggcacct ggcaagggcc tggagtgggt ggccaggatc      960

agaagcaagt acaacaatta tgccacctac tatgccgact ccgtgaagga taggttcaca     1020

atctccagag acgattctaa gaataccgcc tacctgcaga tgaacaatct gaagacagag     1080

gataccgccg tgtactattg cgtgagacac ggcaactttg gcaattctta catcagctat     1140

tgggcctact ggggccaggg cacactggtg accgtgagca gcggcggcgg cggcagcggc     1200

ggcggcggca gcggcggcgg cggcagccag acagtggtga cccaggagcc aagcctgacc     1260

gtgtcccctg gcggcaccgt gacactgacc tgtggatcta gcacaggagc agtgacctcc     1320

ggaaactacc ctaattgggt gcagcagaag ccaggacagg cccctagggg cctgatcggc     1380

ggaacaaagt tcctggcccc aggcaccccc gccagatttt ctggcagcct gctgggcggc     1440

aaggccgccc tgaccctgtc cggagtgcag ccagaggacg aggccgagta ctattgcgtg     1500

ctgtggtaca gcaatcgctg ggtcttcgga gggggaacca aactgactgt cctgcaccac     1560

caccaccacc attgataa                                                   1578


<210>  36
<211>  524
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRDBiTE, operably linked to IgE leader 
       and His tag

<400>  36

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn Ile Glu 
        35                  40                  45              


Asp Tyr Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Met Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr Gly Pro 
65                  70                  75                  80  


Ile Phe Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile Asn Thr 
                85                  90                  95      


Val Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr 
            100                 105                 110         


Tyr Cys Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr Thr Val 
        115                 120                 125             


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro 
145                 150                 155                 160 


Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp 
            180                 185                 190         


Leu Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser Leu Val 
        195                 200                 205             


Ser Lys Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val 
225                 230                 235                 240 


Ala Val Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile 
305                 310                 315                 320 


Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys 
                325                 330                 335     


Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu 
            340                 345                 350         


Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val 
        355                 360                 365             


Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp 
    370                 375                 380                 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu 
                405                 410                 415     


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly 
            420                 425                 430         


Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln 
        435                 440                 445             


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe 
    450                 455                 460                 


Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
465                 470                 475                 480 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
                485                 490                 495     


Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly 
            500                 505                 510         


Thr Lys Leu Thr Val Leu His His His His His His 
        515                 520                 


<210>  37
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE without start or stop codons or
       IgE leader

<400>  37
caggtgcagc tggtcgaatc tggcggcggc ctggtcaagc ccggagagag cctgagactg       60

agttgtgcag caagcgggtt tactttctcc gactactata tgtactgggt gaggcaggca      120

cctggcaagg gcctggagtg ggtggcaagc atctccgacg gcggatctaa cacctactat      180

agcgatatca tcaagggcag gttcacaatc tccagagaca acgccaagaa cagcctgtac      240

ctgcagatga atagcctgaa ggccgaggat accgccgtgt actattgcgc aaggggattc      300

ccactgctga ggcacggcgc ctttgactat tggggccagg gcaccctggt gacagtgagc      360

agcggcggcg gcggcagcgg cggcggcggc agcggcggcg gcggctccga tatccagatg      420

acccagtccc catctagcct gtctgccagc gtgggcgaca gggtgaccat cacatgtaga      480

gcctctcaga acgtggatac aaatgtggca tggtaccagc agaagccagg acaggcccct      540

aagtccctga tctattccgc ctcttaccgg tatagcgatg tgccatcccg cttcagcgga      600

tccgcctctg gcaccgactt taccctgaca atctcctctg tgcagagcga ggattttgcc      660

acatactatt gccagcagta caactcatac ccttacacct tcggaggagg cacaaaactg      720

gaaatcaagt caggcggcgg cggctctgag gtgcagctgg tggagagcgg cggcggcctg      780

gtgcagcccg gcggctccct gaagctgtct tgtgccgcca gcggcttcac cttcaacaag      840

tatgccatga attgggtgag gcaggcacct ggcaagggcc tggagtgggt ggccaggatc      900

agaagcaagt acaacaatta tgccacctac tatgccgact ccgtgaagga taggttcaca      960

atctccagag acgattctaa gaataccgcc tacctgcaga tgaacaatct gaagacagag     1020

gataccgccg tgtactattg cgtgagacac ggcaactttg gcaattctta catcagctat     1080

tgggcctact ggggccaggg cacactggtg accgtgagca gcggcggcgg cggcagcggc     1140

ggcggcggca gcggcggcgg cggcagccag acagtggtga cccaggagcc aagcctgacc     1200

gtgtcccctg gcggcaccgt gacactgacc tgtggatcta gcacaggagc agtgacctcc     1260

ggaaactacc ctaattgggt gcagcagaag ccaggacagg cccctagggg cctgatcggc     1320

ggaacaaagt tcctggcccc aggcaccccc gccagatttt ctggcagcct gctgggcggc     1380

aaggccgccc tgaccctgtc cggagtgcag ccagaggacg aggccgagta ctattgcgtg     1440

ctgtggtaca gcaatcgctg ggtcttcgga gggggaacca aactgactgt cctg           1494


<210>  38
<211>  498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE without start or IgE leader

<400>  38

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Asp Gly Gly Ser Asn Thr Tyr Tyr Ser Asp Ile Ile 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Phe Pro Leu Leu Arg His Gly Ala Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
    130                 135                 140                 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Asn Val Asp Thr Asn Val Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Lys Ser Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser 
            180                 185                 190         


Asp Val Pro Ser Arg Phe Ser Gly Ser Ala Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Val Gln Ser Glu Asp Phe Ala Thr Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Asn Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
                245                 250                 255     


Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala 
            260                 265                 270         


Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val Arg Gln 
        275                 280                 285             


Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr 
    290                 295                 300                 


Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr 
305                 310                 315                 320 


Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn 
                325                 330                 335     


Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn 
            340                 345                 350         


Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln Gly Thr 
        355                 360                 365             


Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 


Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr 
385                 390                 395                 400 


Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly 
                405                 410                 415     


Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly 
            420                 425                 430         


Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly 
        435                 440                 445             


Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu 
    450                 455                 460                 


Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val 
465                 470                 475                 480 


Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr 
                485                 490                 495     


Val Leu 
        


<210>  39
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE, operably linked to IgE leader

<400>  39
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtcaggtg       60

cagctggtcg aatctggcgg cggcctggtc aagcccggag agagcctgag actgagttgt      120

gcagcaagcg ggtttacttt ctccgactac tatatgtact gggtgaggca ggcacctggc      180

aagggcctgg agtgggtggc aagcatctcc gacggcggat ctaacaccta ctatagcgat      240

atcatcaagg gcaggttcac aatctccaga gacaacgcca agaacagcct gtacctgcag      300

atgaatagcc tgaaggccga ggataccgcc gtgtactatt gcgcaagggg attcccactg      360

ctgaggcacg gcgcctttga ctattggggc cagggcaccc tggtgacagt gagcagcggc      420

ggcggcggca gcggcggcgg cggcagcggc ggcggcggct ccgatatcca gatgacccag      480

tccccatcta gcctgtctgc cagcgtgggc gacagggtga ccatcacatg tagagcctct      540

cagaacgtgg atacaaatgt ggcatggtac cagcagaagc caggacaggc ccctaagtcc      600

ctgatctatt ccgcctctta ccggtatagc gatgtgccat cccgcttcag cggatccgcc      660

tctggcaccg actttaccct gacaatctcc tctgtgcaga gcgaggattt tgccacatac      720

tattgccagc agtacaactc atacccttac accttcggag gaggcacaaa actggaaatc      780

aagtcaggcg gcggcggctc tgaggtgcag ctggtggaga gcggcggcgg cctggtgcag      840

cccggcggct ccctgaagct gtcttgtgcc gccagcggct tcaccttcaa caagtatgcc      900

atgaattggg tgaggcaggc acctggcaag ggcctggagt gggtggccag gatcagaagc      960

aagtacaaca attatgccac ctactatgcc gactccgtga aggataggtt cacaatctcc     1020

agagacgatt ctaagaatac cgcctacctg cagatgaaca atctgaagac agaggatacc     1080

gccgtgtact attgcgtgag acacggcaac tttggcaatt cttacatcag ctattgggcc     1140

tactggggcc agggcacact ggtgaccgtg agcagcggcg gcggcggcag cggcggcggc     1200

ggcagcggcg gcggcggcag ccagacagtg gtgacccagg agccaagcct gaccgtgtcc     1260

cctggcggca ccgtgacact gacctgtgga tctagcacag gagcagtgac ctccggaaac     1320

taccctaatt gggtgcagca gaagccagga caggccccta ggggcctgat cggcggaaca     1380

aagttcctgg ccccaggcac ccccgccaga ttttctggca gcctgctggg cggcaaggcc     1440

gccctgaccc tgtccggagt gcagccagag gacgaggccg agtactattg cgtgctgtgg     1500

tacagcaatc gctgggtctt cggaggggga accaaactga ctgtcctgtg ataa           1554


<210>  40
<211>  516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE, operably linked to IgE leader

<400>  40

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro 
            20                  25                  30          


Gly Glu Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
        35                  40                  45              


Asp Tyr Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Ser Ile Ser Asp Gly Gly Ser Asn Thr Tyr Tyr Ser Asp 
65                  70                  75                  80  


Ile Ile Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Gly Phe Pro Leu Leu Arg His Gly Ala Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Asn Val Asp Thr Asn Val Ala Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Gln Ala Pro Lys Ser Leu Ile Tyr Ser Ala Ser Tyr Arg 
        195                 200                 205             


Tyr Ser Asp Val Pro Ser Arg Phe Ser Gly Ser Ala Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Val Gln Ser Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Asn Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
            260                 265                 270         


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser 
        275                 280                 285             


Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val 
    290                 295                 300                 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser 
305                 310                 315                 320 


Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met 
            340                 345                 350         


Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His 
        355                 360                 365             


Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln 
    370                 375                 380                 


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
385                 390                 395                 400 


Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser 
                405                 410                 415     


Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser 
            420                 425                 430         


Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys 
        435                 440                 445             


Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala 
    450                 455                 460                 


Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala 
465                 470                 475                 480 


Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr 
                485                 490                 495     


Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys 
            500                 505                 510         


Leu Thr Val Leu 
        515     


<210>  41
<211>  1572
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE, operably linked to IgE leader 
       and His tag

<400>  41
atggattgga catggattct gtttctggtc gccgccgcta cccgcgtgca tagtcaggtg       60

cagctggtcg aatctggcgg cggcctggtc aagcccggag agagcctgag actgagttgt      120

gcagcaagcg ggtttacttt ctccgactac tatatgtact gggtgaggca ggcacctggc      180

aagggcctgg agtgggtggc aagcatctcc gacggcggat ctaacaccta ctatagcgat      240

atcatcaagg gcaggttcac aatctccaga gacaacgcca agaacagcct gtacctgcag      300

atgaatagcc tgaaggccga ggataccgcc gtgtactatt gcgcaagggg attcccactg      360

ctgaggcacg gcgcctttga ctattggggc cagggcaccc tggtgacagt gagcagcggc      420

ggcggcggca gcggcggcgg cggcagcggc ggcggcggct ccgatatcca gatgacccag      480

tccccatcta gcctgtctgc cagcgtgggc gacagggtga ccatcacatg tagagcctct      540

cagaacgtgg atacaaatgt ggcatggtac cagcagaagc caggacaggc ccctaagtcc      600

ctgatctatt ccgcctctta ccggtatagc gatgtgccat cccgcttcag cggatccgcc      660

tctggcaccg actttaccct gacaatctcc tctgtgcaga gcgaggattt tgccacatac      720

tattgccagc agtacaactc atacccttac accttcggag gaggcacaaa actggaaatc      780

aagtcaggcg gcggcggctc tgaggtgcag ctggtggaga gcggcggcgg cctggtgcag      840

cccggcggct ccctgaagct gtcttgtgcc gccagcggct tcaccttcaa caagtatgcc      900

atgaattggg tgaggcaggc acctggcaag ggcctggagt gggtggccag gatcagaagc      960

aagtacaaca attatgccac ctactatgcc gactccgtga aggataggtt cacaatctcc     1020

agagacgatt ctaagaatac cgcctacctg cagatgaaca atctgaagac agaggatacc     1080

gccgtgtact attgcgtgag acacggcaac tttggcaatt cttacatcag ctattgggcc     1140

tactggggcc agggcacact ggtgaccgtg agcagcggcg gcggcggcag cggcggcggc     1200

ggcagcggcg gcggcggcag ccagacagtg gtgacccagg agccaagcct gaccgtgtcc     1260

cctggcggca ccgtgacact gacctgtgga tctagcacag gagcagtgac ctccggaaac     1320

taccctaatt gggtgcagca gaagccagga caggccccta ggggcctgat cggcggaaca     1380

aagttcctgg ccccaggcac ccccgccaga ttttctggca gcctgctggg cggcaaggcc     1440

gccctgaccc tgtccggagt gcagccagag gacgaggccg agtactattg cgtgctgtgg     1500

tacagcaatc gctgggtctt cggaggggga accaaactga ctgtcctgca ccaccaccac     1560

caccattgat aa                                                         1572


<210>  42
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, PSMADBiTE, operably linked to IgE leader 
       and His tag

<400>  42

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro 
            20                  25                  30          


Gly Glu Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
        35                  40                  45              


Asp Tyr Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Ser Ile Ser Asp Gly Gly Ser Asn Thr Tyr Tyr Ser Asp 
65                  70                  75                  80  


Ile Ile Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Gly Phe Pro Leu Leu Arg His Gly Ala Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Asn Val Asp Thr Asn Val Ala Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Gln Ala Pro Lys Ser Leu Ile Tyr Ser Ala Ser Tyr Arg 
        195                 200                 205             


Tyr Ser Asp Val Pro Ser Arg Phe Ser Gly Ser Ala Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Val Gln Ser Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Asn Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
            260                 265                 270         


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser 
        275                 280                 285             


Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val 
    290                 295                 300                 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser 
305                 310                 315                 320 


Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met 
            340                 345                 350         


Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His 
        355                 360                 365             


Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln 
    370                 375                 380                 


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
385                 390                 395                 400 


Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser 
                405                 410                 415     


Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser 
            420                 425                 430         


Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys 
        435                 440                 445             


Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala 
    450                 455                 460                 


Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala 
465                 470                 475                 480 


Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr 
                485                 490                 495     


Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys 
            500                 505                 510         


Leu Thr Val Leu His His His His His His 
        515                 520         


<210>  43
<211>  1488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE without start, stop or leader 
       sequence

<400>  43
gaagtgcagc tggtcgagag tggaggagga ctggtgcagc caggcggctc tctgaggctg       60

agctgcgcag catccggctt caccttcaac acctacgcaa tgaattgggt gcgccaggca      120

ccaggcaagg gcctggagtg ggtggccagg atcagaagca agtacaacaa ttatgccaca      180

tactatgccg cctctgtgaa gggccggttc accatcagcc gcgacgattc caagaactct      240

ctgtatctgc agatgaacag cctgaagaca gaggacaccg ccgtgtacta ttgcgcccgg      300

cacggcaact tcggcaatag ctacgtgtcc tggtttgcct attggggcca gggcacactg      360

gtgaccgtga gcagcggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc      420

cagacagtgg tgacccagga gccctctctg acagtgagcc ctggcggcac cgtgacactg      480

acctgtcggt ctagcaccgg cgccgtgacc acaagcaact acgccaattg ggtgcagcag      540

aagccaggac aggcccccag gggcctgatc ggcggaacaa acaagagggc accaggaacc      600

cctgcacgct tctccggctc tctgctgggc ggcaaggccg ccctgaccct gtctggagtg      660

cagcccgagg atgaggccga gtactattgc gccctgtggt actccaatct gtgggtgttt      720

ggcggcggaa caaagctgac cgtgctgggc ggcggcggca gcgaggtgca gctggtgcag      780

tccggcgccg aggtgaagaa gcctggcgag tccctgaaga tctcttgtaa gggctctggc      840

tacagcttca catcctattg gatcagctgg gtgaggcaga tgcctggcaa gggcctggaa      900

tggatgggca tcatcgaccc tagcgactcc gataccagat actctccaag ctttcagggc      960

caggtgacaa tctccgccga taagtccatc tctaccgcct atctgcagtg gtcctctctg     1020

aaggcctccg acacagccat gtactattgc gcacgcggcg atggatctac cgacctggat     1080

tactggggac agggcacact ggtcaccgtg agcagcggcg gcggcggcag cggcggcggc     1140

ggcagcggcg gcggcggctc tgagatcgtg ctgacacaga gcccaggcac cctgagcctg     1200

tccccaggag agagggccac cctgtcctgt agagcctctc agagcgtgtc tagctcctac     1260

ctggcctggt atcagcagaa gcctggccag gccccaaggc tgctgatcta cggagcatct     1320

agcagggcaa caggcatccc cgacagattc tccggatctg gaagcggaac cgacttcacc     1380

ctgaccatca gcagactgga gcctgaggac ttcgccgtgt actattgtca gcaggattat     1440

ggctttcctt ggaccttcgg acagggcaca aaagtcgaga ttaagaga                  1488


<210>  44
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE without start or leader 
       sequence

<400>  44

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Ala 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Ser 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val 
    130                 135                 140                 


Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu 
145                 150                 155                 160 


Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn 
                165                 170                 175     


Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly 
            180                 185                 190         


Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu 
        195                 200                 205             


Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp 
    210                 215                 220                 


Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn Leu Trp Val Phe 
225                 230                 235                 240 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser Glu Val 
                245                 250                 255     


Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu Ser Leu 
            260                 265                 270         


Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr Trp Ile 
        275                 280                 285             


Ser Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met Gly Ile 
    290                 295                 300                 


Ile Asp Pro Ser Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe Gln Gly 
305                 310                 315                 320 


Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr Leu Gln 
                325                 330                 335     


Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys Ala Arg 
            340                 345                 350         


Gly Asp Gly Ser Thr Asp Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
        355                 360                 365             


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    370                 375                 380                 


Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu 
385                 390                 395                 400 


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val 
                405                 410                 415     


Ser Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
            420                 425                 430         


Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp 
        435                 440                 445             


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
    450                 455                 460                 


Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Asp Tyr 
465                 470                 475                 480 


Gly Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
                485                 490                 495     


<210>  45
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE, operably linked to IgE leader

<400>  45
atggattgga cttggattct gtttctggtc gccgccgcaa ctagagtgca ttctgaagtg       60

cagctggtcg agagtggagg aggactggtg cagccaggcg gctctctgag gctgagctgc      120

gcagcatccg gcttcacctt caacacctac gcaatgaatt gggtgcgcca ggcaccaggc      180

aagggcctgg agtgggtggc caggatcaga agcaagtaca acaattatgc cacatactat      240

gccgcctctg tgaagggccg gttcaccatc agccgcgacg attccaagaa ctctctgtat      300

ctgcagatga acagcctgaa gacagaggac accgccgtgt actattgcgc ccggcacggc      360

aacttcggca atagctacgt gtcctggttt gcctattggg gccagggcac actggtgacc      420

gtgagcagcg gcggcggcgg cagcggcggc ggcggcagcg gcggcggcgg cagccagaca      480

gtggtgaccc aggagccctc tctgacagtg agccctggcg gcaccgtgac actgacctgt      540

cggtctagca ccggcgccgt gaccacaagc aactacgcca attgggtgca gcagaagcca      600

ggacaggccc ccaggggcct gatcggcgga acaaacaaga gggcaccagg aacccctgca      660

cgcttctccg gctctctgct gggcggcaag gccgccctga ccctgtctgg agtgcagccc      720

gaggatgagg ccgagtacta ttgcgccctg tggtactcca atctgtgggt gtttggcggc      780

ggaacaaagc tgaccgtgct gggcggcggc ggcagcgagg tgcagctggt gcagtccggc      840

gccgaggtga agaagcctgg cgagtccctg aagatctctt gtaagggctc tggctacagc      900

ttcacatcct attggatcag ctgggtgagg cagatgcctg gcaagggcct ggaatggatg      960

ggcatcatcg accctagcga ctccgatacc agatactctc caagctttca gggccaggtg     1020

acaatctccg ccgataagtc catctctacc gcctatctgc agtggtcctc tctgaaggcc     1080

tccgacacag ccatgtacta ttgcgcacgc ggcgatggat ctaccgacct ggattactgg     1140

ggacagggca cactggtcac cgtgagcagc ggcggcggcg gcagcggcgg cggcggcagc     1200

ggcggcggcg gctctgagat cgtgctgaca cagagcccag gcaccctgag cctgtcccca     1260

ggagagaggg ccaccctgtc ctgtagagcc tctcagagcg tgtctagctc ctacctggcc     1320

tggtatcagc agaagcctgg ccaggcccca aggctgctga tctacggagc atctagcagg     1380

gcaacaggca tccccgacag attctccgga tctggaagcg gaaccgactt caccctgacc     1440

atcagcagac tggagcctga ggacttcgcc gtgtactatt gtcagcagga ttatggcttt     1500

ccttggacct tcggacaggg cacaaaagtc gagattaaga gatgataa                  1548


<210>  46
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE, operably linked to IgE leader

<400>  46

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
        35                  40                  45              


Thr Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser 
        115                 120                 125             


Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
145                 150                 155                 160 


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
                165                 170                 175     


Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr 
            180                 185                 190         


Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
        195                 200                 205             


Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
    210                 215                 220                 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
225                 230                 235                 240 


Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn Leu Trp 
                245                 250                 255     


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser 
            260                 265                 270         


Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
        275                 280                 285             


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
    290                 295                 300                 


Trp Ile Ser Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
305                 310                 315                 320 


Gly Ile Ile Asp Pro Ser Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
                325                 330                 335     


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
            340                 345                 350         


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
        355                 360                 365             


Ala Arg Gly Asp Gly Ser Thr Asp Leu Asp Tyr Trp Gly Gln Gly Thr 
    370                 375                 380                 


Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu 
                405                 410                 415     


Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln 
            420                 425                 430         


Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        435                 440                 445             


Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile 
    450                 455                 460                 


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
465                 470                 475                 480 


Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln 
                485                 490                 495     


Asp Tyr Gly Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            500                 505                 510         


Lys Arg 
        


<210>  47
<211>  1566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE, operably linked to IgE leader
       and His tag

<400>  47
atggattgga cttggattct gtttctggtc gccgccgcaa ctagagtgca ttctgaagtg       60

cagctggtcg agagtggagg aggactggtg cagccaggcg gctctctgag gctgagctgc      120

gcagcatccg gcttcacctt caacacctac gcaatgaatt gggtgcgcca ggcaccaggc      180

aagggcctgg agtgggtggc caggatcaga agcaagtaca acaattatgc cacatactat      240

gccgcctctg tgaagggccg gttcaccatc agccgcgacg attccaagaa ctctctgtat      300

ctgcagatga acagcctgaa gacagaggac accgccgtgt actattgcgc ccggcacggc      360

aacttcggca atagctacgt gtcctggttt gcctattggg gccagggcac actggtgacc      420

gtgagcagcg gcggcggcgg cagcggcggc ggcggcagcg gcggcggcgg cagccagaca      480

gtggtgaccc aggagccctc tctgacagtg agccctggcg gcaccgtgac actgacctgt      540

cggtctagca ccggcgccgt gaccacaagc aactacgcca attgggtgca gcagaagcca      600

ggacaggccc ccaggggcct gatcggcgga acaaacaaga gggcaccagg aacccctgca      660

cgcttctccg gctctctgct gggcggcaag gccgccctga ccctgtctgg agtgcagccc      720

gaggatgagg ccgagtacta ttgcgccctg tggtactcca atctgtgggt gtttggcggc      780

ggaacaaagc tgaccgtgct gggcggcggc ggcagcgagg tgcagctggt gcagtccggc      840

gccgaggtga agaagcctgg cgagtccctg aagatctctt gtaagggctc tggctacagc      900

ttcacatcct attggatcag ctgggtgagg cagatgcctg gcaagggcct ggaatggatg      960

ggcatcatcg accctagcga ctccgatacc agatactctc caagctttca gggccaggtg     1020

acaatctccg ccgataagtc catctctacc gcctatctgc agtggtcctc tctgaaggcc     1080

tccgacacag ccatgtacta ttgcgcacgc ggcgatggat ctaccgacct ggattactgg     1140

ggacagggca cactggtcac cgtgagcagc ggcggcggcg gcagcggcgg cggcggcagc     1200

ggcggcggcg gctctgagat cgtgctgaca cagagcccag gcaccctgag cctgtcccca     1260

ggagagaggg ccaccctgtc ctgtagagcc tctcagagcg tgtctagctc ctacctggcc     1320

tggtatcagc agaagcctgg ccaggcccca aggctgctga tctacggagc atctagcagg     1380

gcaacaggca tccccgacag attctccgga tctggaagcg gaaccgactt caccctgacc     1440

atcagcagac tggagcctga ggacttcgcc gtgtactatt gtcagcagga ttatggcttt     1500

ccttggacct tcggacaggg cacaaaagtc gagattaaga gacaccatca ccaccaccac     1560

tgataa                                                                1566


<210>  48
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, CD123DBiTE, operably linked to IgE leader
       and His tag

<400>  48

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
        35                  40                  45              


Thr Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser 
        115                 120                 125             


Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
145                 150                 155                 160 


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
                165                 170                 175     


Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr 
            180                 185                 190         


Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
        195                 200                 205             


Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
    210                 215                 220                 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
225                 230                 235                 240 


Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn Leu Trp 
                245                 250                 255     


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser 
            260                 265                 270         


Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
        275                 280                 285             


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
    290                 295                 300                 


Trp Ile Ser Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
305                 310                 315                 320 


Gly Ile Ile Asp Pro Ser Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
                325                 330                 335     


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
            340                 345                 350         


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
        355                 360                 365             


Ala Arg Gly Asp Gly Ser Thr Asp Leu Asp Tyr Trp Gly Gln Gly Thr 
    370                 375                 380                 


Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu 
                405                 410                 415     


Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln 
            420                 425                 430         


Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        435                 440                 445             


Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile 
    450                 455                 460                 


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
465                 470                 475                 480 


Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln 
                485                 490                 495     


Asp Tyr Gly Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            500                 505                 510         


Lys Arg His His His His His His 
        515                 520 


<210>  49
<211>  1485
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, nucleotide sequence of HER2DBiTE, without
       start or stop codons or IgE leader

<400>  49
gatattcaga tgactcagag cccttcctcc ctgagcgcct ccgtcggcga tcgggtgacc       60

atcacttgta aggcaagcca ggatgtgagc atcggcgtcg catggtacca gcagaagccc      120

ggcaaagccc caaagctgct gatctattcc gcaagttatc ggtacaccgg cgtcccctca      180

cgcttcagtg gatctgggtc tgggacagac ttcactctga ccattagctc cctgcagcca      240

gaagattttg ccacatacta ctgtcagcag tactatatct atccttacac tttcggacag      300

ggaacaaaag tggagatcaa gcgcggcggg ggagggagcg gaggaggggg ctctggaggc      360

ggcgggagcg aggtgcagct ggtcgagtct gggggaggcc tggtgcagcc cggagggtcc      420

ctgaggctgt cctgtgctgc aagcggcttt actttcacag actacaccat ggactgggtc      480

agacaggctc ccgggaaggg gctggaatgg gtggccgatg tgaaccctaa ctctggcggc      540

agtatctaca accagcggtt taaggggaga ttcacactgt cagtggacag gagcaaaaat      600

acactgtacc tgcagatgaa cagcctgagg gcagaagata cagcagtgta ctactgtgcc      660

agaaatctgg gacccagttt ttatttcgat tactgggggc agggaacact ggtgacagtg      720

tcatccggcg gcgggggctc tgaagtccag ctggtggaga gcggaggagg actggtccag      780

cctggcggaa gcctgcggct gtcctgcgct gccagcggat atagttttac cggctacact      840

atgaactggg tgcgccaggc acccggaaaa ggactggagt gggtcgctct gatcaacccc      900

tataagggag tctcaactta caatcagaag tttaaagatc gcttcactat cagtgtggac      960

aagtccaaga atacagccta tctgcagatg aactccctgc gcgccgaaga tactgccgtc     1020

tactattgcg cacggtcagg atattacgga gacagtgact ggtatttcga tgtctgggga     1080

caggggacac tggtgacagt ctcctctggc gggggaggct ccgggggagg aggatcagga     1140

ggcggcggat ctgacattca gatgactcag tcacctagta gcctgtctgc tagcgtggga     1200

gacagggtca ctattacctg cagggccagc caggatatcc gcaattacct gaactggtat     1260

cagcagaaac caggcaaagc ccccaaactg ctgatctact atacctcccg gctggaatca     1320

ggggtccctt caaggttctc aggctccgga agtggcaccg attacactct gacaattagc     1380

tcactgcagc cagaagactt tgctacctac tactgccagc aggggaatac tctgccttgg     1440

accttcggcc agggaactaa agtggagatt aagtcatcct gataa                     1485


<210>  50
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE, without start or IgE leader

<400>  50

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Gly 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
        115                 120                 125             


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
    130                 135                 140                 


Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp Trp Val 
145                 150                 155                 160 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp Val Asn Pro 
                165                 170                 175     


Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly Arg Phe Thr 
            180                 185                 190         


Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser 
        195                 200                 205             


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Leu Gly 
    210                 215                 220                 


Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
225                 230                 235                 240 


Ser Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
                245                 250                 255     


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
            260                 265                 270         


Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Arg Gln Ala Pro 
        275                 280                 285             


Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Asn Pro Tyr Lys Gly Val 
    290                 295                 300                 


Ser Thr Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Val Asp 
305                 310                 315                 320 


Lys Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
                325                 330                 335     


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser 
            340                 345                 350         


Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        355                 360                 365             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
385                 390                 395                 400 


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 
                405                 410                 415     


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
            420                 425                 430         


Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
        435                 440                 445             


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
    450                 455                 460                 


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Trp 
465                 470                 475                 480 


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Ser 
                485                 490             


<210>  51
<211>  1539
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE, with IgE leader

<400>  51
atggactgga cttggattct gttcctggtc gcagcagcca cacgggtgca ttccgatatt       60

cagatgactc agagcccttc ctccctgagc gcctccgtcg gcgatcgggt gaccatcact      120

tgtaaggcaa gccaggatgt gagcatcggc gtcgcatggt accagcagaa gcccggcaaa      180

gccccaaagc tgctgatcta ttccgcaagt tatcggtaca ccggcgtccc ctcacgcttc      240

agtggatctg ggtctgggac agacttcact ctgaccatta gctccctgca gccagaagat      300

tttgccacat actactgtca gcagtactat atctatcctt acactttcgg acagggaaca      360

aaagtggaga tcaagcgcgg cgggggaggg agcggaggag ggggctctgg aggcggcggg      420

agcgaggtgc agctggtcga gtctggggga ggcctggtgc agcccggagg gtccctgagg      480

ctgtcctgtg ctgcaagcgg ctttactttc acagactaca ccatggactg ggtcagacag      540

gctcccggga aggggctgga atgggtggcc gatgtgaacc ctaactctgg cggcagtatc      600

tacaaccagc ggtttaaggg gagattcaca ctgtcagtgg acaggagcaa aaatacactg      660

tacctgcaga tgaacagcct gagggcagaa gatacagcag tgtactactg tgccagaaat      720

ctgggaccca gtttttattt cgattactgg gggcagggaa cactggtgac agtgtcatcc      780

ggcggcgggg gctctgaagt ccagctggtg gagagcggag gaggactggt ccagcctggc      840

ggaagcctgc ggctgtcctg cgctgccagc ggatatagtt ttaccggcta cactatgaac      900

tgggtgcgcc aggcacccgg aaaaggactg gagtgggtcg ctctgatcaa cccctataag      960

ggagtctcaa cttacaatca gaagtttaaa gatcgcttca ctatcagtgt ggacaagtcc     1020

aagaatacag cctatctgca gatgaactcc ctgcgcgccg aagatactgc cgtctactat     1080

tgcgcacggt caggatatta cggagacagt gactggtatt tcgatgtctg gggacagggg     1140

acactggtga cagtctcctc tggcggggga ggctccgggg gaggaggatc aggaggcggc     1200

ggatctgaca ttcagatgac tcagtcacct agtagcctgt ctgctagcgt gggagacagg     1260

gtcactatta cctgcagggc cagccaggat atccgcaatt acctgaactg gtatcagcag     1320

aaaccaggca aagcccccaa actgctgatc tactatacct cccggctgga atcaggggtc     1380

ccttcaaggt tctcaggctc cggaagtggc accgattaca ctctgacaat tagctcactg     1440

cagccagaag actttgctac ctactactgc cagcagggga atactctgcc ttggaccttc     1500

ggccagggaa ctaaagtgga gattaagtca tcctgataa                            1539


<210>  52
<211>  511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE, with IgE leader

<400>  52

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            20                  25                  30          


Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser 
        35                  40                  45              


Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    50                  55                  60                  


Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
                85                  90                  95      


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr 
            100                 105                 110         


Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
    130                 135                 140                 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp Val 
            180                 185                 190         


Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly Arg 
        195                 200                 205             


Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn 
225                 230                 235                 240 


Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
                245                 250                 255     


Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
            260                 265                 270         


Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
        275                 280                 285             


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Arg Gln 
    290                 295                 300                 


Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Asn Pro Tyr Lys 
305                 310                 315                 320 


Gly Val Ser Thr Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser 
                325                 330                 335     


Val Asp Lys Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg 
            340                 345                 350         


Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly 
        355                 360                 365             


Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr 
    370                 375                 380                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
385                 390                 395                 400 


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
                405                 410                 415     


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg 
            420                 425                 430         


Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        435                 440                 445             


Leu Ile Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe 
    450                 455                 460                 


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu 
465                 470                 475                 480 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu 
                485                 490                 495     


Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Ser 
            500                 505                 510     


<210>  53
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, CD3xHer2 HER2DBiTE with His6 tag

<400>  53
atggactgga cttggagaat cctgttcctg gtcgctgccg ccactggaac tcacgcccag       60

attgtgctga ctcagagccc tgctattatg tccgcctctc ccggcgagaa ggtgaccatg      120

acctgttctg cctccagctc cgtgtcctat gacggcgata gctacatgaa ctggtaccag      180

cagaagtctg gcacctctcc caagagatgg atctacgata ccagcaagct ggccagcggc      240

gtgcccgccc actttcgggg cagcggctcc ggcaccagct actctctgac aatctccggc      300

atggaggccg aggatgccgc cacctactac tgccagcagt ggtcctctaa tcctttcacc      360

ttcggctctg gcacaaagct ggagatcaat cgcggcggcg gcggctccgg cggcggcggc      420

agcggcggcg gcggctccca ggtgcagctg cagcagtctg gcgccgagct ggcccgccct      480

ggcgcctctg tgaagatgtc ttgtaaggcc tccggctaca ccttcaccag atataccatg      540

cactgggtga agcagcgccc aggccagggc ctggagtgga tcggctatat caatccatct      600

agaggctaca ccaattacaa ccagaagttc aaggataagg ccaccctgac cacagacaag      660

tcctcctcta cagcctacat gcagctgtcc tctctgacat ccgaggattc cgccgtgtac      720

tattgtgcca gatattacga cgatcactac tgtctggact attggggcca gggcacaacc      780

ctgacagtgt ccagcggcgg cggcggctct gaggtgcagc tggtggagtc tggcggcggc      840

ctggtgcagc ccggcggctc tctgagactg tcttgtgccg cctctggctt taccttcaca      900

gattatacca tggattgggt gagacaggcc cctggcaagg gcctggagtg ggtggccgat      960

gtgaacccca atagcggcgg ctccatctac aatcagaggt ttaagggccg ctttacactg     1020

tctgtggacc ggtctaagaa caccctgtac ctgcagatga attctctgag agccgaggac     1080

accgccgtgt attactgtgc caggaatctg ggcccttctt tctatttcga ctactggggc     1140

cagggcacac tggtgaccgt gtcctccggc ggcggcggca gcggcggcgg cggctctggc     1200

ggcggcggca gcgacatcca gatgacccag tccccaagct ccctgtctgc ctccgtgggc     1260

gatcgcgtga ccatcacatg caaggcctcc caggacgtgt ctatcggcgt ggcctggtac     1320

cagcagaagc ctggcaaggc cccaaagctg ctgatctact ctgcctccta tagatacacc     1380

ggcgtgccct ctagattctc cggctctggc tctggcaccg acttcacact gaccatctct     1440

tctctgcagc ccgaggactt cgccacctac tattgtcagc agtactacat ctacccttac     1500

accttcggac aggggacaaa agtggaaatc aagagacatc accatcacca tcattgataa     1560


<210>  54
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, CD3xHer2 HER2DBiTE with His6 tag

<400>  54

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala 
            20                  25                  30          


Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val 
        35                  40                  45              


Ser Tyr Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly 
    50                  55                  60                  


Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly 
65                  70                  75                  80  


Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
                85                  90                  95      


Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Asn Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro 
145                 150                 155                 160 


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                165                 170                 175     


Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
            180                 185                 190         


Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln 
        195                 200                 205             


Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr 
    210                 215                 220                 


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
225                 230                 235                 240 


Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly 
                245                 250                 255     


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val 
            260                 265                 270         


Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
        275                 280                 285             


Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met 
    290                 295                 300                 


Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp 
305                 310                 315                 320 


Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly 
                325                 330                 335     


Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln 
            340                 345                 350         


Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
        355                 360                 365             


Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
    370                 375                 380                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
                405                 410                 415     


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp 
            420                 425                 430         


Val Ser Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        435                 440                 445             


Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser 
    450                 455                 460                 


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
465                 470                 475                 480 


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr 
                485                 490                 495     


Ile Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            500                 505                 510         


His His His His His His 
        515             


<210>  55
<211>  1539
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, CD3xHer2 HER2DBiTE with IgE leader

<400>  55
atggattgga cctggattct gtttctggtg gcggcggcga cccgcgtgca tagccagatt       60

gtgctgactc agagccctgc tattatgtcc gcctctcccg gcgagaaggt gaccatgacc      120

tgttctgcct ccagctccgt gtcctatgac ggcgatagct acatgaactg gtaccagcag      180

aagtctggca cctctcccaa gagatggatc tacgatacca gcaagctggc cagcggcgtg      240

cccgcccact ttcggggcag cggctccggc accagctact ctctgacaat ctccggcatg      300

gaggccgagg atgccgccac ctactactgc cagcagtggt cctctaatcc tttcaccttc      360

ggctctggca caaagctgga gatcaatcgc ggcggcggcg gctccggcgg cggcggcagc      420

ggcggcggcg gctcccaggt gcagctgcag cagtctggcg ccgagctggc ccgccctggc      480

gcctctgtga agatgtcttg taaggcctcc ggctacacct tcaccagata taccatgcac      540

tgggtgaagc agcgcccagg ccagggcctg gagtggatcg gctatatcaa tccatctaga      600

ggctacacca attacaacca gaagttcaag gataaggcca ccctgaccac agacaagtcc      660

tcctctacag cctacatgca gctgtcctct ctgacatccg aggattccgc cgtgtactat      720

tgtgccagat attacgacga tcactactgt ctggactatt ggggccaggg cacaaccctg      780

acagtgtcca gcggcggcgg cggctctgag gtgcagctgg tggagtctgg cggcggcctg      840

gtgcagcccg gcggctctct gagactgtct tgtgccgcct ctggctttac cttcacagat      900

tataccatgg attgggtgag acaggcccct ggcaagggcc tggagtgggt ggccgatgtg      960

aaccccaata gcggcggctc catctacaat cagaggttta agggccgctt tacactgtct     1020

gtggaccggt ctaagaacac cctgtacctg cagatgaatt ctctgagagc cgaggacacc     1080

gccgtgtatt actgtgccag gaatctgggc ccttctttct atttcgacta ctggggccag     1140

ggcacactgg tgaccgtgtc ctccggcggc ggcggcagcg gcggcggcgg ctctggcggc     1200

ggcggcagcg acatccagat gacccagtcc ccaagctccc tgtctgcctc cgtgggcgat     1260

cgcgtgacca tcacatgcaa ggcctcccag gacgtgtcta tcggcgtggc ctggtaccag     1320

cagaagcctg gcaaggcccc aaagctgctg atctactctg cctcctatag atacaccggc     1380

gtgccctcta gattctccgg ctctggctct ggcaccgact tcacactgac catctcttct     1440

ctgcagcccg aggacttcgc cacctactat tgtcagcagt actacatcta cccttacacc     1500

ttcggacagg ggacaaaagt ggaaatcaag agatgataa                            1539


<210>  56
<211>  511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, CD3xHer2 HER2DBiTE with IgE leader

<400>  56

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser 
            20                  25                  30          


Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser 
        35                  40                  45              


Tyr Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
    50                  55                  60                  


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
            100                 105                 110         


Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Asn Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly 
145                 150                 155                 160 


Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg 
                165                 170                 175     


Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys 
        195                 200                 205             


Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
225                 230                 235                 240 


Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln 
                245                 250                 255     


Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp Val 
305                 310                 315                 320 


Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly Arg 
                325                 330                 335     


Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln Met 
            340                 345                 350         


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn 
        355                 360                 365             


Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
    370                 375                 380                 


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
                405                 410                 415     


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val 
            420                 425                 430         


Ser Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
        435                 440                 445             


Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg 
    450                 455                 460                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
465                 470                 475                 480 


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile 
                485                 490                 495     


Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            500                 505                 510     


<210>  57
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE-L

<400>  57
atggattgga cctggatcct gtttctggtg gccgctgcca caagagtgca cagcgaagtg       60

cagctggtgg aatctggcgg aggactggtt caacctggcg gctctctgag actgtcttgt      120

gccgccagcg gcttcacctt caacacctac gccatgaact gggtccgaca ggcccctggc      180

aaaggccttg aatgggtcgc cagaatcaga agcaagtaca acaattacgc cacctactac      240

gccgcctccg tgaagggcag attcaccatc agcagggacg acagcaagaa cagcctgtac      300

ctgcagatga actccctgaa aaccgaggac accgccgtgt actactgtgc cagacacggc      360

aacttcggca acagctatgt gtcttggttt gcctactggg gccagggcac actggtcaca      420

gtttctagcg gaggcggagg ttctggcggc ggaggaagtg gtggcggagg ctctcaaaca      480

gtggtcaccc aagagcctag cctgaccgtt tctcctggcg gaaccgtgac actgacctgt      540

agatcttcta caggcgccgt gaccaccagc aactacgcca attgggtgca gcagaagcca      600

ggccaggctc ctagaggact gatcggaggc accaacaaga gagcccctgg aacaccagcc      660

agattctctg gctctctgct cggaggaaag gccgctctga cactgagcgg agtgcagcct      720

gaagatgagg ccgagtacta ttgcgccctg tggtacagca acctgtgggt tttcggcgga      780

ggcacaaagc tgacagtgct tggaggtggc ggatccgagg ttcagctggt tgaaagtggc      840

ggtggcttgg tgcaaccagg cggaagcctc agacttagct gtgccgcctc tggctttacc      900

tttaccgact acacaatgga ttgggttcga caagctccag gcaagggact cgagtgggtc      960

gccgatgtga atcctaatag cggcggcagc atctacaacc agcggttcaa aggccggttc     1020

accctgagcg tggacagatc caagaatact ctgtatctcc agatgaatag cctgcgcgcc     1080

gaggatacag ctgtgtatta ctgcgcccgg aatctgggcc ccagcttcta ctttgattat     1140

tggggacagg gaaccctcgt gaccgtgtct agtggcggag gtggaagtgg cggaggcggt     1200

tcaggtggcg gaggatctga tatccagatg acacagagcc ccagcagcct gtctgcctct     1260

gtgggagaca gagtgaccat cacatgcaag gccagccagg acgtgtccat tggcgtggca     1320

tggtatcagc agaaacccgg caaggcccct aagctgctga tctacagcgc cagctacaga     1380

tacaccggcg tgccctctag attttccggc agcggcagcg gaaccgactt taccctgaca     1440

atcagctccc tgcagccaga ggacttcgcc acctattact gccagcagta ctacatctac     1500

ccctacacct tcggacaggg caccaaggtg gaaatcaagc gg                        1542


<210>  58
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE-L

<400>  58

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
        35                  40                  45              


Thr Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser 
        115                 120                 125             


Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
145                 150                 155                 160 


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
                165                 170                 175     


Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr 
            180                 185                 190         


Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
        195                 200                 205             


Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
    210                 215                 220                 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
225                 230                 235                 240 


Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn Leu Trp 
                245                 250                 255     


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser 
            260                 265                 270         


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
        275                 280                 285             


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
    290                 295                 300                 


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
305                 310                 315                 320 


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
                325                 330                 335     


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
            340                 345                 350         


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
        355                 360                 365             


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
    370                 375                 380                 


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
                405                 410                 415     


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
            420                 425                 430         


Gln Asp Val Ser Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        435                 440                 445             


Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val 
    450                 455                 460                 


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
465                 470                 475                 480 


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
                485                 490                 495     


Tyr Tyr Ile Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            500                 505                 510         


Lys Arg 
        


<210>  59
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE-L with His tag

<400>  59
atggattgga cctggatcct gtttctggtg gccgctgcca caagagtgca cagcgaagtg       60

cagctggtgg aatctggcgg aggactggtt caacctggcg gctctctgag actgtcttgt      120

gccgccagcg gcttcacctt caacacctac gccatgaact gggtccgaca ggcccctggc      180

aaaggccttg aatgggtcgc cagaatcaga agcaagtaca acaattacgc cacctactac      240

gccgcctccg tgaagggcag attcaccatc agcagggacg acagcaagaa cagcctgtac      300

ctgcagatga actccctgaa aaccgaggac accgccgtgt actactgtgc cagacacggc      360

aacttcggca acagctatgt gtcttggttt gcctactggg gccagggcac actggtcaca      420

gtttctagcg gaggcggagg ttctggcggc ggaggaagtg gtggcggagg ctctcaaaca      480

gtggtcaccc aagagcctag cctgaccgtt tctcctggcg gaaccgtgac actgacctgt      540

agatcttcta caggcgccgt gaccaccagc aactacgcca attgggtgca gcagaagcca      600

ggccaggctc ctagaggact gatcggaggc accaacaaga gagcccctgg aacaccagcc      660

agattctctg gctctctgct cggaggaaag gccgctctga cactgagcgg agtgcagcct      720

gaagatgagg ccgagtacta ttgcgccctg tggtacagca acctgtgggt tttcggcgga      780

ggcacaaagc tgacagtgct tggaggtggc ggatccgagg ttcagctggt tgaaagtggc      840

ggtggcttgg tgcaaccagg cggaagcctc agacttagct gtgccgcctc tggctttacc      900

tttaccgact acacaatgga ttgggttcga caagctccag gcaagggact cgagtgggtc      960

gccgatgtga atcctaatag cggcggcagc atctacaacc agcggttcaa aggccggttc     1020

accctgagcg tggacagatc caagaatact ctgtatctcc agatgaatag cctgcgcgcc     1080

gaggatacag ctgtgtatta ctgcgcccgg aatctgggcc ccagcttcta ctttgattat     1140

tggggacagg gaaccctcgt gaccgtgtct agtggcggag gtggaagtgg cggaggcggt     1200

tcaggtggcg gaggatctga tatccagatg acacagagcc ccagcagcct gtctgcctct     1260

gtgggagaca gagtgaccat cacatgcaag gccagccagg acgtgtccat tggcgtggca     1320

tggtatcagc agaaacccgg caaggcccct aagctgctga tctacagcgc cagctacaga     1380

tacaccggcg tgccctctag attttccggc agcggcagcg gaaccgactt taccctgaca     1440

atcagctccc tgcagccaga ggacttcgcc acctattact gccagcagta ctacatctac     1500

ccctacacct tcggacaggg caccaaggtg gaaatcaagc ggcaccacca ccatcaccac     1560


<210>  60
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, HER2DBiTE-L with His tag

<400>  60

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
        35                  40                  45              


Thr Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser 
        115                 120                 125             


Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr 
145                 150                 155                 160 


Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val 
                165                 170                 175     


Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr 
            180                 185                 190         


Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile 
        195                 200                 205             


Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly 
    210                 215                 220                 


Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro 
225                 230                 235                 240 


Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn Leu Trp 
                245                 250                 255     


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser 
            260                 265                 270         


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
        275                 280                 285             


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
    290                 295                 300                 


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
305                 310                 315                 320 


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
                325                 330                 335     


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
            340                 345                 350         


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
        355                 360                 365             


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
    370                 375                 380                 


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
                405                 410                 415     


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
            420                 425                 430         


Gln Asp Val Ser Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        435                 440                 445             


Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val 
    450                 455                 460                 


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
465                 470                 475                 480 


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
                485                 490                 495     


Tyr Tyr Ile Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            500                 505                 510         


Lys Arg His His His His His His 
        515                 520 


<210>  61
<211>  2190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, human Her2DMAb

<400>  61
atggattgga catggattct gtttctggtc gccgccgcca caagagtgca ttccgaagtg       60

cagctggtcg agtcaggggg aggactggtg cagcccggcg gaagcctgcg actgtcctgc      120

gccgcttctg gcttcacttt taccgactac accatggatt gggtgcgcca ggcacctggg      180

aagggactgg aatgggtcgc tgatgtgaac ccaaatagtg ggggctcaat ctacaaccag      240

cgattcaaag gcaggtttac cctgagtgtg gacagatcaa agaacacact gtatctgcag      300

atgaatagcc tgagggcaga ggataccgcc gtgtactatt gcgccagaaa tctgggacca      360

tccttctact ttgactattg gggacagggg actctggtca ccgtgagctc cgcctcaaca      420

aaaggcccca gcgtgttccc cctggctcct tctagtaagt ctacaagtgg agggactgca      480

gccctgggat gtctggtgaa ggactacttc cctgagccag tcaccgtgag ctggaactcc      540

ggcgctctga cttctggagt ccacaccttt cccgcagtgc tgcagtcaag cgggctgtac      600

tccctgtcct ctgtggtcac cgtccctagt tcaagcctgg gcacacagac ttatatctgc      660

aacgtgaatc acaaaccatc taatacaaag gtcgacaaga aagtggaacc caaaagctgt      720

gataagaccc atacatgccc tccctgtcca gctcctgagc tgctgggcgg accaagcgtg      780

ttcctgtttc cacccaagcc taaagatacc ctgatgattt cccggacccc agaagtcaca      840

tgcgtggtcg tggacgtgtc tcacgaggac cccgaagtca agtttaactg gtacgtggac      900

ggcgtcgagg tgcataatgc caagacaaaa ccacgcgagg aacagtacaa ctccacttat      960

cgagtcgtgt ctgtcctgac cgtgctgcat caggattggc tgaacgggaa ggagtataag     1020

tgcaaagtga gcaacaaggc cctgccagct cccatcgaga agaccatttc caaggcaaaa     1080

ggccagccac gggaacccca ggtgtacaca ctgcctccat ctcgcgatga gctgaccaaa     1140

aaccaggtca gtctgacatg tctggtgaag ggcttctatc cctcagacat cgccgtggag     1200

tgggaatcca atggacagcc tgaaaacaat tacaagacca caccccctgt gctggactct     1260

gatggaagtt tctttctgta tagtaaactg actgtggata agtcaaggtg gcagcagggg     1320

aacgtctttt catgcagcgt gatgcacgag gccctgcaca atcattacac acagaaatcc     1380

ctgtctctga gtcctggaaa acgggggcgc aagaggagat caggcagcgg agcaacaaac     1440

ttcagcctgc tgaagcaggc aggggacgtg gaggaaaatc ctggcccaat ggattggact     1500

tggattctgt tcctggtcgc tgcagccact agggtgcata gcgacattca gatgacccag     1560

tccccctcct ctctgtccgc ctctgtcggc gacagagtga ctatcacctg taaggctagc     1620

caggatgtct ccattggagt ggcatggtac cagcagaagc ctgggaaagc tccaaagctg     1680

ctgatctaca gtgcatcata cagatataca ggagtgcctt cccggttcag cgggtccggc     1740

tctggaactg actttacact gactatcagt tcactgcagc ctgaggattt cgctacctac     1800

tattgccagc agtactatat ctacccatat acatttgggc agggcactaa agtggaaatc     1860

aagcggactg tcgctgcacc tagcgtgttc atctttccac ccagtgatga gcagctgaag     1920

tctggaaccg ccagtgtggt gtgcctgctg aacaatttct acccacgcga agccaaagtc     1980

cagtggaagg tggacaacgc tctgcagtca gggaatagcc aggagtccgt gacagaacag     2040

gactctaaag atagtactta ttcactgagc tccaccctga cactgtccaa ggcagactac     2100

gagaagcaca aagtgtatgc ctgcgaggtc actcaccagg ggctgtcttc acccgtcact     2160

aagtccttta atagagggga atgttgataa                                      2190


<210>  62
<211>  728
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, human Her2DMAb

<400>  62

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr 
        35                  40                  45              


Asp Tyr Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln 
65                  70                  75                  80  


Arg Phe Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser Gly Ser Gly Ala Thr Asn 
465                 470                 475                 480 


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
                485                 490                 495     


Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
            500                 505                 510         


His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
        515                 520                 525             


Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser 
    530                 535                 540                 


Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
545                 550                 555                 560 


Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe 
                565                 570                 575     


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
            580                 585                 590         


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr 
        595                 600                 605             


Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
    610                 615                 620                 


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
625                 630                 635                 640 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
                645                 650                 655     


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
            660                 665                 670         


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
        675                 680                 685             


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
    690                 695                 700                 


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
705                 710                 715                 720 


Lys Ser Phe Asn Arg Gly Glu Cys 
                725             


<210>  63
<211>  2187
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, mouse Her2DMAb

<400>  63
atggattgga catggatcct gttcctggtg gccgctgcca ccagggtgca cagcgaggtg       60

cagctggtgg agtccggcgg aggcctggtg cagccaggag gctctctgag gctgagctgc      120

gctgcctccg gcttcacctt tacagactac accatggatt gggtgagaca ggctccagga      180

aagggcctgg agtgggtggc tgacgtgaac cctaactccg gaggctctat ctacaaccag      240

aggttcaagg gcaggttcac cctgtctgtg gaccgcagca agaacaccct gtacctgcag      300

atgaactccc tgcgggccga ggatacagcc gtgtactact gtgcccgcaa cctgggacct      360

tctttctact ttgactactg gggacagggc acactggtga ccgtgagctc cgccaagacc      420

acagctccat ccgtgtaccc actggctccc gtgtgcggcg acaccacagg atctagcgtg      480

accctgggct gtctggtgaa gggatacttc cccgagcctg tgaccctgac atggaacagc      540

ggctccctgt cctctggagt gcacacattt cctgccgtgc tgcagagcga tctgtacacc      600

ctgagctcct ctgtgaccgt gacaagctcc acatggcctt ctcagagcat cacctgcaac      660

gtggcccacc cagcttctag cacaaaggtg gacaagaaga tcgagcctcg gggcccaacc      720

atcaagcctt gtcccccttg caagtgtcca gctccaaacc tgctgggagg cccatccgtg      780

ttcatctttc cacccaagat caaggatgtg ctgatgatct ccctgtctcc aatcgtgacc      840

tgcgtggtgg tggacgtgtc cgaggacgat cccgatgtgc agatctcttg gttcgtgaac      900

aacgtggagg tgcacacagc ccagacccag acacacaggg aggactacaa ctccaccctg      960

agagtggtgt ctgctctgcc aatccagcac caggactgga tgagcggcaa ggagtttaag     1020

tgcaaggtga acaacaagga tctgcctgcc ccaatcgaga ggacaatctc taagccaaag     1080

ggaagcgtga gagctcccca ggtgtacgtg ctgcctccac ccgaggagga gatgacaaag     1140

aagcaggtga ccctgacatg tatggtgacc gacttcatgc cagaggatat ctacgtggag     1200

tggacaaaca acggcaagac cgagctgaac tacaagaaca ccgagcccgt gctggacagc     1260

gatggatcct actttatgta ctccaagctg cgggtggaga agaagaactg ggtggagcgc     1320

aacagctact cctgctctgt ggtgcacgag ggcctgcaca accaccacac cacaaagagc     1380

ttctccagga cacccggcaa gcggggacgc aagaggagat ctggaagcgg cgccaccaac     1440

tttagcctgc tgaagcaggc tggcgacgtg gaggagaacc caggacctat ggattggacc     1500

tggatcctgt tcctggtcgc tgccgctaca cgggtgcact ctgacatcca gatgacccag     1560

agcccatcct ctctgtccgc ctctgtgggc gaccgcgtga ccatcacctg taaggcttcc     1620

caggacgtga gcatcggagt ggcttggtac cagcagaagc ccggaaaggc ccctaagctg     1680

ctgatctaca gcgcctccta ccggtacaca ggagtgccat cccgcttctc tggaagcgga     1740

tccggaaccg actttaccct gacaatcagc tccctgcagc ccgaggattt cgccacctac     1800

tactgccagc agtactacat ctacccttac acatttggcc agggaaccaa ggtggagatc     1860

aaggccgacg ccgctcccac agtgagcatc ttccctccat ctagcgagca gctgaccagc     1920

ggaggcgctt ccgtggtgtg cttcctgaac aacttttacc ctaaggacat caacgtgaag     1980

tggaagatcg atggcagcga gagacagaac ggagtgctga actcctggac agaccaggat     2040

tctaaggaca gcacctactc catgtcctct accctgacac tgaccaagga tgagtacgag     2100

aggcacaact cttacacatg cgaggccacc cacaagacat ctaccagccc tatcgtgaag     2160

agctttaaca gaaacgagtg ttgataa                                         2187


<210>  64
<211>  727
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, mouse Her2DMAb

<400>  64

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr 
        35                  40                  45              


Asp Tyr Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln 
65                  70                  75                  80  


Arg Phe Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser 
    130                 135                 140                 


Val Tyr Pro Leu Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val 
145                 150                 155                 160 


Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu 
                165                 170                 175     


Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr 
        195                 200                 205             


Ser Ser Thr Trp Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro 
    210                 215                 220                 


Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr 
225                 230                 235                 240 


Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met 
            260                 265                 270         


Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu 
        275                 280                 285             


Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val 
    290                 295                 300                 


His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu 
305                 310                 315                 320 


Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly 
                325                 330                 335     


Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val 
        355                 360                 365             


Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr 
    370                 375                 380                 


Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu 
385                 390                 395                 400 


Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val 
            420                 425                 430         


Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val 
        435                 440                 445             


His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr 
    450                 455                 460                 


Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser Gly Ser Gly Ala Thr Asn 
465                 470                 475                 480 


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
                485                 490                 495     


Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
            500                 505                 510         


His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
        515                 520                 525             


Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser 
    530                 535                 540                 


Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
545                 550                 555                 560 


Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe 
                565                 570                 575     


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
            580                 585                 590         


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr 
        595                 600                 605             


Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Asp Ala 
    610                 615                 620                 


Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser 
625                 630                 635                 640 


Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp 
                645                 650                 655     


Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val 
            660                 665                 670         


Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met 
        675                 680                 685             


Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser 
    690                 695                 700                 


Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys 
705                 710                 715                 720 


Ser Phe Asn Arg Asn Glu Cys 
                725         


<210>  65
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, scFv Her2DMAb

<400>  65
gaggtgcagc tggtggagtc tggcggcggc ctggtgcagc ccggcggctc tctgagactg       60

tcttgtgccg cctctggctt taccttcaca gattatacca tggattgggt gagacaggcc      120

cctggcaagg gcctggagtg ggtggccgat gtgaacccca atagcggcgg ctccatctac      180

aatcagaggt ttaagggccg ctttacactg tctgtggacc ggtctaagaa caccctgtac      240

ctgcagatga attctctgag agccgaggac accgccgtgt attactgtgc caggaatctg      300

ggcccttctt tctatttcga ctactggggc cagggcacac tggtgaccgt gtcctccggc      360

ggcggcggca gcggcggcgg cggctctggc ggcggcggca gcgacatcca gatgacccag      420

tccccaagct ccctgtctgc ctccgtgggc gatcgcgtga ccatcacatg caaggcctcc      480

caggacgtgt ctatcggcgt ggcctggtac cagcagaagc ctggcaaggc cccaaagctg      540

ctgatctact ctgcctccta tagatacacc ggcgtgccct ctagattctc cggctctggc      600

tctggcaccg acttcacact gaccatctct tctctgcagc ccgaggactt cgccacctac      660

tattgtcagc agtactacat ctacccttac accttcggac aggggacaaa agtggaaatc      720

aagaga                                                                 726


<210>  66
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, scFv Her2DMAb

<400>  66

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
    50                  55                  60                  


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 


Gln Asp Val Ser Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     


Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val 
            180                 185                 190         


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 


Tyr Tyr Ile Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 


Lys 
    


<210>  67
<211>  4493
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, pGX93237 full plasmid sequence

<400>  67
gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccgccac catggactgg acttggattc tgttcctggt cgcagcagcc      780

acacgggtgc attccgatat tcagatgact cagagccctt cctccctgag cgcctccgtc      840

ggcgatcggg tgaccatcac ttgtaaggca agccaggatg tgagcatcgg cgtcgcatgg      900

taccagcaga agcccggcaa agccccaaag ctgctgatct attccgcaag ttatcggtac      960

accggcgtcc cctcacgctt cagtggatct gggtctggga cagacttcac tctgaccatt     1020

agctccctgc agccagaaga ttttgccaca tactactgtc agcagtacta tatctatcct     1080

tacactttcg gacagggaac aaaagtggag atcaagcgcg gcgggggagg gagcggagga     1140

gggggctctg gaggcggcgg gagcgaggtg cagctggtcg agtctggggg aggcctggtg     1200

cagcccggag ggtccctgag gctgtcctgt gctgcaagcg gctttacttt cacagactac     1260

accatggact gggtcagaca ggctcccggg aaggggctgg aatgggtggc cgatgtgaac     1320

cctaactctg gcggcagtat ctacaaccag cggtttaagg ggagattcac actgtcagtg     1380

gacaggagca aaaatacact gtacctgcag atgaacagcc tgagggcaga agatacagca     1440

gtgtactact gtgccagaaa tctgggaccc agtttttatt tcgattactg ggggcaggga     1500

acactggtga cagtgtcatc cggcggcggg ggctctgaag tccagctggt ggagagcgga     1560

ggaggactgg tccagcctgg cggaagcctg cggctgtcct gcgctgccag cggatatagt     1620

tttaccggct acactatgaa ctgggtgcgc caggcacccg gaaaaggact ggagtgggtc     1680

gctctgatca acccctataa gggagtctca acttacaatc agaagtttaa agatcgcttc     1740

actatcagtg tggacaagtc caagaataca gcctatctgc agatgaactc cctgcgcgcc     1800

gaagatactg ccgtctacta ttgcgcacgg tcaggatatt acggagacag tgactggtat     1860

ttcgatgtct ggggacaggg gacactggtg acagtctcct ctggcggggg aggctccggg     1920

ggaggaggat caggaggcgg cggatctgac attcagatga ctcagtcacc tagtagcctg     1980

tctgctagcg tgggagacag ggtcactatt acctgcaggg ccagccagga tatccgcaat     2040

tacctgaact ggtatcagca gaaaccaggc aaagccccca aactgctgat ctactatacc     2100

tcccggctgg aatcaggggt cccttcaagg ttctcaggct ccggaagtgg caccgattac     2160

actctgacaa ttagctcact gcagccagaa gactttgcta cctactactg ccagcagggg     2220

aatactctgc cttggacctt cggccaggga actaaagtgg agattaagtc atcctgataa     2280

ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc     2340

cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc     2400

actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct     2460

attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg     2520

catgctgggg atgcggtggg ctctatggct tctactgggc ggttttatgg acagcaagcg     2580

aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact     2640

ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga     2700

caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg     2760

cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg     2820

ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt     2880

ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg     2940

gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat     3000

tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat     3060

ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg     3120

accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg     3180

atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc     3240

tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc     3300

cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg     3360

tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg     3420

gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca     3480

tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga     3540

tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt     3600

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat     3660

ccgctcatga gacaataacc ctgataaatg cttcaataat agcacgtgct aaaacttcat     3720

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     3780

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     3840

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     3900

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     3960

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     4020

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     4080

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     4140

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     4200

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     4260

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     4320

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     4380

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     4440

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctt            4493


<210>  68
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized, G4S linker

<400>  68

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  69
<211>  1506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRvIII DNA encoded immune cell engager

<400>  69
gatgtcgtga tgacccagag ccctgatagc ctggccgtga gcctgggaga gagggcaacc       60

atcaactgca agagctccca gagcctgctg gactccgatg gcaagacata cctgaattgg      120

ctgcagcaga agcctggcca gccccctaag aggctgatct ctctggtgag caagctggac      180

tccggagtgc cagatcgctt cagcggctcc ggctctggaa ccgactttac cctgacaatc      240

tctagcctgc aggccgagga tgtggccgtg tactattgct ggcagggcac acacttcccc      300

ggcacctttg gcggcggcac aaaggtggag atcaagggcg gcggcggctc tggaggagga      360

ggcagcggcg gaggaggctc cggaggcggc ggctctgaga tccagctggt gcagagcgga      420

gcagaggtga agaagccagg cgagtctctg agaatcagct gtaagggctc cggcttcaac      480

atcgaggact actatatcca ctgggtgcgg cagatgcctg gcaagggcct ggagtggatg      540

ggcagaatcg acccagagaa cgatgagaca aagtatggcc ccatcttcca gggccacgtg      600

acaatctctg ccgacacaag catcaatacc gtgtacctgc agtggtcctc tctgaaggcc      660

tccgataccg ccatgtacta ttgcgccttt aggggaggcg tgtattgggg acagggaacc      720

acagtgacag tgagctccgg aggaggaggc agcgaggtgc agctggtgga gagcggcggc      780

ggcctggtgc agccaggcgg ctccctgaag ctgtcttgtg ccgccagcgg cttcaccttt      840

aacaagtacg caatgaattg ggtgcgccag gcaccaggca agggcctgga gtgggtggcc      900

cggatcagat ctaagtacaa caattatgcc acatactatg ccgacagcgt gaaggatagg      960

ttcaccatct cccgcgacga ttctaagaac acagcctacc tgcagatgaa caatctgaag     1020

acagaggaca ccgccgtgta ctattgcgtg aggcacggca actttggcaa ttcctacatc     1080

tcttattggg cctactgggg ccagggcaca ctggtgaccg tgtctagcgg cggcggcggc     1140

agcggcggcg gcggctccgg aggaggcggc tctcagacag tggtgaccca ggagccaagc     1200

ctgaccgtgt cccctggagg caccgtgaca ctgacctgtg gctcctctac aggagcagtg     1260

accagcggaa actatcctaa ttgggtgcag cagaagcctg gacaggcacc acggggcctg     1320

atcggaggaa ccaagttcct ggcaccagga acacccgccc ggttcagcgg ctccctgctg     1380

ggaggcaagg ccgccctgac cctgtccggc gtgcagccag aggatgaggc cgagtactat     1440

tgcgtgctgt ggtactctaa tcggtgggtc tttggcgggg gaactaaact gactgtcctg     1500

tgataa                                                                1506


<210>  70
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRvIII DNA encoded immune cell engager

<400>  70

Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Gln Gln Lys Pro Gly Gln Pro 
        35                  40                  45              


Pro Lys Arg Leu Ile Ser Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Gly Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
    130                 135                 140                 


Lys Pro Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn 
145                 150                 155                 160 


Ile Glu Asp Tyr Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly 
                165                 170                 175     


Leu Glu Trp Met Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr 
            180                 185                 190         


Gly Pro Ile Phe Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile 
        195                 200                 205             


Asn Thr Val Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala 
    210                 215                 220                 


Met Tyr Tyr Cys Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr 
225                 230                 235                 240 


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
                245                 250                 255     


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser 
            260                 265                 270         


Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn Trp Val 
        275                 280                 285             


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser 
    290                 295                 300                 


Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg 
305                 310                 315                 320 


Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met 
                325                 330                 335     


Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His 
            340                 345                 350         


Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp Gly Gln 
        355                 360                 365             


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser 
385                 390                 395                 400 


Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser 
                405                 410                 415     


Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys 
            420                 425                 430         


Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala 
        435                 440                 445             


Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala 
    450                 455                 460                 


Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr 
465                 470                 475                 480 


Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys 
                485                 490                 495     


Leu Thr Val Leu 
            500 


<210>  71
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRvIII DNA encoded immune cell engager 
       with IgE leader

<400>  71
atggattgga cttggattct gttcctggtg gcagccgcaa ctagagtgca ttccgatgtc       60

gtgatgaccc agagccctga tagcctggcc gtgagcctgg gagagagggc aaccatcaac      120

tgcaagagct cccagagcct gctggactcc gatggcaaga catacctgaa ttggctgcag      180

cagaagcctg gccagccccc taagaggctg atctctctgg tgagcaagct ggactccgga      240

gtgccagatc gcttcagcgg ctccggctct ggaaccgact ttaccctgac aatctctagc      300

ctgcaggccg aggatgtggc cgtgtactat tgctggcagg gcacacactt ccccggcacc      360

tttggcggcg gcacaaaggt ggagatcaag ggcggcggcg gctctggagg aggaggcagc      420

ggcggaggag gctccggagg cggcggctct gagatccagc tggtgcagag cggagcagag      480

gtgaagaagc caggcgagtc tctgagaatc agctgtaagg gctccggctt caacatcgag      540

gactactata tccactgggt gcggcagatg cctggcaagg gcctggagtg gatgggcaga      600

atcgacccag agaacgatga gacaaagtat ggccccatct tccagggcca cgtgacaatc      660

tctgccgaca caagcatcaa taccgtgtac ctgcagtggt cctctctgaa ggcctccgat      720

accgccatgt actattgcgc ctttagggga ggcgtgtatt ggggacaggg aaccacagtg      780

acagtgagct ccggaggagg aggcagcgag gtgcagctgg tggagagcgg cggcggcctg      840

gtgcagccag gcggctccct gaagctgtct tgtgccgcca gcggcttcac ctttaacaag      900

tacgcaatga attgggtgcg ccaggcacca ggcaagggcc tggagtgggt ggcccggatc      960

agatctaagt acaacaatta tgccacatac tatgccgaca gcgtgaagga taggttcacc     1020

atctcccgcg acgattctaa gaacacagcc tacctgcaga tgaacaatct gaagacagag     1080

gacaccgccg tgtactattg cgtgaggcac ggcaactttg gcaattccta catctcttat     1140

tgggcctact ggggccaggg cacactggtg accgtgtcta gcggcggcgg cggcagcggc     1200

ggcggcggct ccggaggagg cggctctcag acagtggtga cccaggagcc aagcctgacc     1260

gtgtcccctg gaggcaccgt gacactgacc tgtggctcct ctacaggagc agtgaccagc     1320

ggaaactatc ctaattgggt gcagcagaag cctggacagg caccacgggg cctgatcgga     1380

ggaaccaagt tcctggcacc aggaacaccc gcccggttca gcggctccct gctgggaggc     1440

aaggccgccc tgaccctgtc cggcgtgcag ccagaggatg aggccgagta ctattgcgtg     1500

ctgtggtact ctaatcggtg ggtctttggc gggggaacta aactgactgt cctgtgataa     1560


<210>  72
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, EGFRvIII DNA encoded immune cell engager 
       with IgE leader

<400>  72

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser 
            20                  25                  30          


Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu 
        35                  40                  45              


Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Arg Leu Ile Ser Leu Val Ser Lys Leu Asp Ser Gly 
65                  70                  75                  80  


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Trp 
            100                 105                 110         


Gln Gly Thr His Phe Pro Gly Thr Phe Gly Gly Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Ile Gln Leu Val Gln Ser Gly Ala Glu 
145                 150                 155                 160 


Val Lys Lys Pro Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly 
                165                 170                 175     


Phe Asn Ile Glu Asp Tyr Tyr Ile His Trp Val Arg Gln Met Pro Gly 
            180                 185                 190         


Lys Gly Leu Glu Trp Met Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr 
        195                 200                 205             


Lys Tyr Gly Pro Ile Phe Gln Gly His Val Thr Ile Ser Ala Asp Thr 
    210                 215                 220                 


Ser Ile Asn Thr Val Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp 
225                 230                 235                 240 


Thr Ala Met Tyr Tyr Cys Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln 
                245                 250                 255     


Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Lys 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr Ala Met Asn 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile 
305                 310                 315                 320 


Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys 
                325                 330                 335     


Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu 
            340                 345                 350         


Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val 
        355                 360                 365             


Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp Ala Tyr Trp 
    370                 375                 380                 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val Thr Gln Glu 
                405                 410                 415     


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly 
            420                 425                 430         


Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln 
        435                 440                 445             


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe 
    450                 455                 460                 


Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
465                 470                 475                 480 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
                485                 490                 495     


Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly 
            500                 505                 510         


Thr Lys Leu Thr Val Leu 
        515             


<210>  73
<211>  1461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Her2 DNA encoded immune cell engager

<400>  73
gacatccaga tgacccagag ccccagcagc ctgagcgcca gcgtgggcga cagggtgacc       60

atcacctgca aggccagcca ggacgtgagc atcggcgtgg cctggtacca gcagaagccc      120

ggcaaggccc ccaagctgct gatctacagc gccagctaca ggtacaccgg cgtgcccagc      180

aggttcagcg gcagcggcag cggcaccgac ttcaccctga ccatcagcag cctgcagccc      240

gaggacttcg ccacctacta ctgccagcag tactacatct acccctacac cttcggccag      300

ggcaccaagg tggagatcaa gggcggcggc ggcagcggcg gcggcggcag cggcggcggc      360

ggcagcgagg tgcagctggt ggagagcggc ggcggcctgg tgcagcccgg cggcagcctg      420

aggctgagct gcgccgccag cggcttcacc ttcaccgact acaccatgga ctgggtgagg      480

caggcccccg gcaagggcct ggagtgggtg gccgacgtga accccaacag cggcggcagc      540

atctacaacc agaggttcaa gggcaggttc accctgagcg tggacaggag caagaacacc      600

ctgtacctgc agatgaacag cctgagggcc gaggacaccg ccgtgtacta ctgcgccagg      660

aacctgggcc ccagcttcta cttcgactac tggggccagg gcaccctggt gaccgtgagc      720

agcggcggcg gcggcagcca ggtgcagctg gtggagagcg gcggcggcgt ggtgcagccc      780

ggcaggagcc tgaggctgag ctgcgccgcc agcggcttca agttcagcgg ctacggcatg      840

cactgggtga ggcaggcccc cggcaagggc ctggagtggg tggccgtgat ctggtacgac      900

ggcagcaaga agtactacgt ggacagcgtg aagggcaggt tcaccatcag cagggacaac      960

agcaagaaca ccctgtacct gcagatgaac agcctgaggg ccgaggacac cgccgtgtac     1020

tactgcgcca ggcagatggg ctactggcac ttcgacctgt ggggcagggg caccctggtg     1080

accgtgagca gcggcggcgg cggcagcggc ggcggcggca gcggcggcgg cggcagcgag     1140

atcgtgctga cccagagccc cgccaccctg agcctgagcc ccggcgagag ggccaccctg     1200

agctgcaggg ccagccagag cgtgagcagc tacctggcct ggtaccagca gaagcccggc     1260

caggccccca ggctgctgat ctacgacgcc agcaacaggg ccaccggcat ccccgccagg     1320

ttcagcggca gcggcagcgg caccgacttc accctgacca tcagcagcct ggagcccgag     1380

gacttcgccg tgtactactg ccagcagagg agcaactggc cccccctgac cttcggcggc     1440

ggcaccaagg tgatcgagaa g                                               1461


<210>  74
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Her2 DNA encoded immune cell engager

<400>  74

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Gly 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
        115                 120                 125             


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
    130                 135                 140                 


Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp Trp Val 
145                 150                 155                 160 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp Val Asn Pro 
                165                 170                 175     


Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly Arg Phe Thr 
            180                 185                 190         


Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser 
        195                 200                 205             


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Leu Gly 
    210                 215                 220                 


Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
225                 230                 235                 240 


Ser Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
                245                 250                 255     


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
            260                 265                 270         


Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Arg Gln Ala Pro 
        275                 280                 285             


Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Asn Pro Tyr Lys Gly Val 
    290                 295                 300                 


Ser Thr Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Val Asp 
305                 310                 315                 320 


Lys Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
                325                 330                 335     


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser 
            340                 345                 350         


Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        355                 360                 365             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
385                 390                 395                 400 


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 
                405                 410                 415     


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
            420                 425                 430         


Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
        435                 440                 445             


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
    450                 455                 460                 


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Trp 
465                 470                 475                 480 


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Ser 
                485                 490             


<210>  75
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Her2 DNA encoded immune cell engager with
       IgE leader

<400>  75
atggactgga cctggatcct gttcctggtg gccgccgcca ccagggtgca cagcgacatc       60

cagatgaccc agagccccag cagcctgagc gccagcgtgg gcgacagggt gaccatcacc      120

tgcaaggcca gccaggacgt gagcatcggc gtggcctggt accagcagaa gcccggcaag      180

gcccccaagc tgctgatcta cagcgccagc tacaggtaca ccggcgtgcc cagcaggttc      240

agcggcagcg gcagcggcac cgacttcacc ctgaccatca gcagcctgca gcccgaggac      300

ttcgccacct actactgcca gcagtactac atctacccct acaccttcgg ccagggcacc      360

aaggtggaga tcaagggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc      420

gaggtgcagc tggtggagag cggcggcggc ctggtgcagc ccggcggcag cctgaggctg      480

agctgcgccg ccagcggctt caccttcacc gactacacca tggactgggt gaggcaggcc      540

cccggcaagg gcctggagtg ggtggccgac gtgaacccca acagcggcgg cagcatctac      600

aaccagaggt tcaagggcag gttcaccctg agcgtggaca ggagcaagaa caccctgtac      660

ctgcagatga acagcctgag ggccgaggac accgccgtgt actactgcgc caggaacctg      720

ggccccagct tctacttcga ctactggggc cagggcaccc tggtgaccgt gagcagcggc      780

ggcggcggca gccaggtgca gctggtggag agcggcggcg gcgtggtgca gcccggcagg      840

agcctgaggc tgagctgcgc cgccagcggc ttcaagttca gcggctacgg catgcactgg      900

gtgaggcagg cccccggcaa gggcctggag tgggtggccg tgatctggta cgacggcagc      960

aagaagtact acgtggacag cgtgaagggc aggttcacca tcagcaggga caacagcaag     1020

aacaccctgt acctgcagat gaacagcctg agggccgagg acaccgccgt gtactactgc     1080

gccaggcaga tgggctactg gcacttcgac ctgtggggca ggggcaccct ggtgaccgtg     1140

agcagcggcg gcggcggcag cggcggcggc ggcagcggcg gcggcggcag cgagatcgtg     1200

ctgacccaga gccccgccac cctgagcctg agccccggcg agagggccac cctgagctgc     1260

agggccagcc agagcgtgag cagctacctg gcctggtacc agcagaagcc cggccaggcc     1320

cccaggctgc tgatctacga cgccagcaac agggccaccg gcatccccgc caggttcagc     1380

ggcagcggca gcggcaccga cttcaccctg accatcagca gcctggagcc cgaggacttc     1440

gccgtgtact actgccagca gaggagcaac tggccccccc tgaccttcgg cggcggcacc     1500

aaggtgatcg agaag                                                      1515


<210>  76
<211>  511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Her2 DNA encoded immune cell engager with
       IgE leader

<400>  76

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            20                  25                  30          


Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser 
        35                  40                  45              


Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    50                  55                  60                  


Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
                85                  90                  95      


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr 
            100                 105                 110         


Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
    130                 135                 140                 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asp Val 
            180                 185                 190         


Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys Gly Arg 
        195                 200                 205             


Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn 
225                 230                 235                 240 


Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
                245                 250                 255     


Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
            260                 265                 270         


Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
        275                 280                 285             


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Arg Gln 
    290                 295                 300                 


Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Asn Pro Tyr Lys 
305                 310                 315                 320 


Gly Val Ser Thr Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser 
                325                 330                 335     


Val Asp Lys Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg 
            340                 345                 350         


Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly 
        355                 360                 365             


Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr 
    370                 375                 380                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
385                 390                 395                 400 


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
                405                 410                 415     


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg 
            420                 425                 430         


Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        435                 440                 445             


Leu Ile Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe 
    450                 455                 460                 


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu 
465                 470                 475                 480 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu 
                485                 490                 495     


Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Ser 
            500                 505                 510     


