                         SEQUENCE LISTING

<110>  Duke University
       Heaton, Nicholas  S.
 
<120>  STABILIZED 9 AND 10 SEGMENTED INFLUENZA VIRUSES AS A VACCINE 
       PLATFORM AND METHODS OF MAKING AND USING SAME

<130>  155554.00529

<150>  62/802,385
<151>  2019-02-07

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PA 5' packaging signal

<400>  1
agcgaaagca ggtactgatc caaattggaa gattttgtgc gacattgctt caatccgttg       60

attgtcgagc ttgcggaaaa aacattgaaa gagtttgggg aggacctgaa aatcgaaaca      120

aacaaattt                                                              129


<210>  2
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PA 3' packaging signal

<400>  2
cttgaacctg ggacctttga tcttgggggg ctatatgaag caattgagga gtgcctgatt       60

aatgatccct gggttttgct taatgcttct tggttcaact ccttccttac acatgcattg      120

agttagttgt ggcagtgcta ctatttgcta tccatactgt ccaaaaaagt accttgtttc      180

tact                                                                   184


<210>  3
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- NA 5' packaging signal

<400>  3
agcgaaagca ggggtttaaa ttgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatttgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatttgcaac caa             173


<210>  4
<211>  209
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- NA 3' packaging signal

<400>  4
tgagctaaca gggctagact gtatgaggcc gtgcttctgg gttgaattaa tcaggggacg       60

acctaaagaa aaaacaatct ggactagtgc gagcagcatt tctttttgtg gcgtgaatag      120

tgatactgta gattggtctt ggccagacgg tgctgagttg ccattcagca ttgacaagta      180

gtctgttcaa aaaactcctt gtttctact                                        209


<210>  5
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB1 5' packaging signal

<400>  5
agcgaaagca ggcaaaccat ttgattggtt gtcaatccga ccttactttt cttaaaagtg       60

ccagcacaaa ttgctataag cacaactttc ccttatactg gagaccctcc ttacagcctt      120

gggacaggaa caggatacac cttgga                                           146


<210>  6
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB1 3' packaging signal

<400>  6
cccgaattga tgcacggatt gatttcgaat ctggaaggat aaagaaagaa gagttcactg       60

agatcatgaa gatctgttcc accattgaag agctcagacg gcaaaaatag tgaatttagc      120

ttgtccttca tgaaaaaatg ccttgtttct act                                   153


<210>  7
<211>  152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 5' packaging signal

<400>  7
agcgaaagca ggtcaattat attcaatttg gaaagaataa aagaactaag aaatctattg       60

tcgcagtctc gcacccgcga gatactcaca aaaaccaccg tggaccattt ggccataatc      120

aagaagtaca catcaggaag acaggagaag aa                                    152


<210>  8
<211>  163
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 3' packaging signal

<400>  8
aaaggagaga aggctaatgt gctaattggg caaggagacg tggtgttggt aatgaaacgg       60

aaacgggact ctagcatact tactgacagc cagacagcga ccaaaagaat tcggatggcc      120

atcaattagt gtcgaatagt ttaaaaacga ccttgtttct act                        163


<210>  9
<211>  1030
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- mCherry with PB1 packaging signals (A/Puerto 
       Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(146)
<223>  PB1 5' packaging signal

<220>
<221>  misc_feature
<222>  (147)..(152)
<223>  EcoRV site

<220>
<221>  misc_feature
<222>  (153)..(158)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (159)..(869)
<223>  Open reading frame of mCherry

<220>
<221>  misc_feature
<222>  (870)..(877)
<223>  PmeI site

<220>
<221>  misc_feature
<222>  (878)..(1030)
<223>  PB1 3' packaging signal

<400>  9
agcgaaagca ggcaaaccat ttgattggtt gtcaatccga ccttactttt cttaaaagtg       60

ccagcacaaa ttgctataag cacaactttc ccttatactg gagaccctcc ttacagcctt      120

gggacaggaa caggatacac cttggagata tcgccaccat ggtctcaaaa ggggaagaag      180

acaatatggc tatcattaag gaatttatgc gatttaaagt tcacatggaa ggaagtgtca      240

atggccatga gtttgagatt gagggagaag gcgaaggaag accatatgag gggactcaga      300

ctgctaaatt aaaagtgaca aagggaggtc ccttaccatt tgcatgggac attctttctc      360

cccagttcat gtatggatcc aaagcatatg ttaagcatcc tgctgatatt cccgattatt      420

tgaaactctc atttccagaa ggtttcaaat gggagagagt catgaacttt gaagatggag      480

gagttgttac tgtcacccaa gattcttctc tccaggatgg ggaatttatt tacaaagtca      540

agctccgagg aacaaatttc ccaagtgatg gaccagtcat gcaaaagaag acaatgggat      600

gggaagcaag ctcagaacgg atgtatcctg aagatggtgc tcttaaaggc gagataaaac      660

aaagacttaa gcttaaagac ggaggacatt atgatgcaga agtaaagacc acttacaagg      720

ctaagaaacc agtacaactg cccggagctt ataatgtcaa tattaaattg gatatcacca      780

gtcacaatga agactacact attgttgaac aatatgaaag agctgaaggt agacatagta      840

ctggtggaat ggatgaactt tataaatgag tttaaacccc gaattgatgc acggattgat      900

ttcgaatctg gaaggataaa gaaagaagag ttcactgaga tcatgaagat ctgttccacc      960

attgaagagc tcagacggca aaaatagtga atttagcttg tccttcatga aaaaatgcct     1020

tgtttctact                                                            1030


<210>  10
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sfGFP with PB2 packaging signals (A/Puerto 
       Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(152)
<223>  PB2 5' packaging signal.

<220>
<221>  misc_feature
<222>  (153)..(158)
<223>  NheI site

<220>
<221>  misc_feature
<222>  (159)..(164)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (165)..(884)
<223>  Open reading frame of sfGFP

<220>
<221>  misc_feature
<222>  (885)..(890)
<223>  XhoI site

<220>
<221>  misc_feature
<222>  (891)..(1053)
<223>  PB2 3' packaging signal

<400>  10
agcgaaagca ggtcaattat attcaatttg gaaagaataa aagaactaag aaatctattg       60

tcgcagtctc gcacccgcga gatactcaca aaaaccaccg tggaccattt ggccataatc      120

aagaagtaca catcaggaag acaggagaag aagctagcgc caccatggtc tcaaaaggag      180

aagaattatt cactggtgtg gtcccaattc ttgttgaact tgatggagac gtcaatggac      240

ataagttttc agtgagaggg gaaggtgagg gcgatgcaac taatggaaaa ttaacactca      300

aatttatttg tactactggg aagctgcccg taccatggcc cactctcgtc accacgctta      360

cttacggggt tcagtgcttc tccaggtacc ccgatcatat gaaacaacat gacttcttca      420

aatcagctat gccagaaggc tatgttcagg agagaaccat ttcattcaag gatgatggca      480

cctataagac cagagccgag gtcaagtttg aaggagatac tcttgtcaat agaattgagc      540

ttaaagggat tgattttaag gaagatggta atattcttgg ccataaattg gagtataatt      600

tcaacagcca taatgtatac atcaccgctg ataaacagaa gaatggcatt aaagcaaatt      660

ttaaaattag gcataatgtc gaggatggaa gtgttcaact tgctgaccat tatcagcaga      720

acacacccat tggagatgga ccagtccttc ttcctgataa ccattaccta tcaacccaat      780

ccgtcttgag taaagaccct aatgagaaga gagaccacat ggttctcctt gaatttgtaa      840

ctgctgctgg aattactctc ggaatggatg aactttataa atgactcgag aaaggagaga      900

aggctaatgt gctaattggg caaggagacg tggtgttggt aatgaaacgg aaacgggact      960

ctagcatact tactgacagc cagacagcga ccaaaagaat tcggatggcc atcaattagt     1020

gtcgaatagt ttaaaaacga ccttgtttct act                                  1053


<210>  11
<211>  2676
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB1 with NA packaging signals (A/Puerto Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(173)
<223>  NA 5' packaging signal

<220>
<221>  misc_feature
<222>  (174)..(179)
<223>  EcoRV site

<220>
<221>  misc_feature
<222>  (180)..(185)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (186)..(2459)
<223>  Open reading frame of PB1

<220>
<221>  misc_feature
<222>  (186)..(260)
<223>  Region of the PB1 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2378)..(2459)
<223>  Region of the PB1 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2460)..(2467)
<223>  PmeI site

<220>
<221>  misc_feature
<222>  (2468)..(2676)
<223>  NA 3' packaging signal

<400>  11
agcgaaagca ggggtttaaa ttgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatttgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatttgcaac caagatatcg      180

ccaccatgga cgtgaaccct actttgcttt tcctgaaagt acctgcccag aatgctattt      240

ccactacatt tccatacacc ggagaccctc cttacagcca tgggacagga acaggataca      300

ccatggatac tgtcaacagg acacatcagt actcagaaaa gggaagatgg acaacaaaca      360

ccgaaactgg agcaccgcaa ctcaacccga ttgatgggcc actgccagaa gacaatgaac      420

caagtggtta tgcccaaaca gattgtgtat tggaagcaat ggctttcctt gaggaatccc      480

atcctggtat ttttgaaaac tcgtgtattg aaacgatgga ggttgttcag caaacacgag      540

tagacaagct gacacaaggc cgacagacct atgactggac tctaaataga aaccaacctg      600

ctgcaacagc attggccaac acaatagaag tgttcagatc aaatggcctc acggccaatg      660

agtctggaag gctcatagac ttccttaagg atgtaatgga gtcaatgaaa aaagaagaaa      720

tggggatcac aactcatttt cagagaaaga gacgggtgag agacaatatg actaagaaaa      780

tgataacaca gagaacaata ggtaaaaaga agcagagatt gaacaaaagg agttatctaa      840

ttagagcatt gaccctgaac acaatgacca aagatgctga gagagggaag ctaaaacgga      900

gagcaattgc aaccccaggg atgcaaataa gggggtttgt atactttgtt gagacactgg      960

caaggagtat atgtgagaaa cttgaacaat cagggttgcc agttggaggc aatgagaaga     1020

aagcaaagtt ggcaaatgtt gtaaggaaga tgatgaccaa ttctcaggac accgaacttt     1080

ctttcaccat cactggagat aacaccaaat ggaacgaaaa tcagaatcct cggatgtttt     1140

tggccatgat cacatatatg acaagaaatc agcccgaatg gttcagaaat gttctaagta     1200

ttgctccaat aatgttctca aacaaaatgg cgagactggg aaaagggtat atgtttgaga     1260

gcaagagtat gaaacttaga actcaaatac ctgcagaaat gctagcaagc atcgatttga     1320

aatatttcaa tgattcaaca agaaagaaga ttgaaaaaat ccgaccgctc ttaatagagg     1380

ggactgcatc attgagccct ggaatgatga tgggcatgtt caatatgtta agcactgtat     1440

taggcgtctc catcctgaat cttggacaaa agagatacac caagactact tactggtggg     1500

atggtcttca atcctctgac gattttgctc tgattgtgaa tgcacccaat catgaaggga     1560

ttcaagccgg agtcgacagg ttttatcgaa cctgtaagct acttggaatc aatatgagca     1620

agaaaaagtc ttacataaac agaacaggta catttgaatt cacaagtttt ttctatcgtt     1680

atgggtttgt tgccaatttc agcatggagc tccccagttt tggggtgtct gggatcaacg     1740

agtcagcgga catgagtatt ggagttactg tcatcaaaaa caatatgata aacaatgatc     1800

ttggtccagc aacagctcaa atggcccttc agttgttcat caaagattac aggtacacgt     1860

accgatgcca tagaggtgac acacaaatac aaacccgaag atcatttgaa ataaagaaac     1920

tgtgggagca aacccgttcc aaagctggac tgctggtctc cgacggaggc ccaaatttat     1980

acaacattag aaatctccac attcctgaag tctgcctaaa atgggaattg atggatgagg     2040

attaccaggg gcgtttatgc aacccactga acccatttgt cagccataaa gaaattgaat     2100

caatgaacaa tgcagtgatg atgccagcac atggtccagc caaaaacatg gagtatgatg     2160

ctgttgcaac aacacactcc tggatcccca aaagaaatcg atccatcttg aatacaagtc     2220

aaagaggagt acttgaagat gaacaaatgt accaaaggtg ctgcaattta tttgaaaaat     2280

tcttccccag cagttcatac agaagaccag tcgggatatc cagtatggtg gaggctatgg     2340

tttccagagc ccgaattgat gcacggattg atttcgaatc aggcagaatc aagaaggagg     2400

aatttacaga gattatgaaa atatgctcaa ccatagagga acttcgaagg caaaaatagg     2460

tttaaactga gctaacaggg ctagactgta tgaggccgtg cttctgggtt gaattaatca     2520

ggggacgacc taaagaaaaa acaatctgga ctagtgcgag cagcatttct ttttgtggcg     2580

tgaatagtga tactgtagat tggtcttggc cagacggtgc tgagttgcca ttcagcattg     2640

acaagtagtc tgttcaaaaa actccttgtt tctact                               2676


<210>  12
<211>  2613
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 with PA packaging signals (A/Puerto Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(129)
<223>  PA 5' packaging signal

<220>
<221>  misc_feature
<222>  (130)..(135)
<223>  EcoRV site

<220>
<221>  misc_feature
<222>  (136)..(141)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (142)..(2421)
<223>  Open reading frame of PB2

<220>
<221>  misc_feature
<222>  (142)..(171)
<223>  Region of the PB2 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2336)..(2421)
<223>  Region of the PB2 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2422)..(2429)
<223>  PMeI site

<220>
<221>  misc_feature
<222>  (2430)..(2613)
<223>  PA 3' packaging signal

<400>  12
agcgaaagca ggtactgatc caaattggaa gattttgtgc gacattgctt caatccgttg       60

attgtcgagc ttgcggaaaa aacattgaaa gagtttgggg aggacctgaa aatcgaaaca      120

aacaaatttg atatcgccac catggagcgg atcaaggagt tgcggaactt gatgtcgcag      180

tctcgcaccc gcgagatact cacaaaaacc accgtggacc atatggccat aatcaagaag      240

tacacatcag gaagacagga gaagaaccca gcacttagga tgaaatggat gatggcaatg      300

aaatatccaa ttacagcaga caagaggata acggaaatga ttcctgagag aaatgagcaa      360

ggacaaactt tatggagtaa aatgaatgat gcaggatcag accgagtgat ggtatcacct      420

ctggctgtga catggtggaa taggaatgga ccaataacaa atacagttca ttatccaaaa      480

atctacaaaa cttattttga aagagtcgaa aggctaaagc atggaacctt tggccctgtc      540

cattttagaa accaagtcaa aatacgtcgg agagttgaca taaatcctgg tcatgcagat      600

ctcagtgcca aggaggcaca ggatgtaatc atggaagttg ttttccctaa cgaagtggga      660

gccaggatac taacatcgga atcgcaacta acgataacca aagagaagaa agaagaactc      720

caggattgca aaatttctcc tttgatggtt gcatacatgt tggagagaga actggtccgc      780

aaaacgagat tcctcccagt ggctggtgga acaagcagtg tgtacattga agtgttgcat      840

ttgactcaag gaacatgctg ggaacagatg tatactccag gaggggaagt gaggaatgat      900

gatgttgatc aaagcttgat tattgctgct aggaacatag tgagaagagc tgcagtatca      960

gcagatccac tagcatcttt attggagatg tgccacagca cacagattgg tggaattagg     1020

atggtagaca tccttaggca gaacccaaca gaagagcaag ccgtggatat atgcaaggct     1080

gcaatgggac tgagaattag ctcatccttc agttttggtg gattcacatt taagagaaca     1140

agcggatcat cagtcaagag agaggaagag gtgcttacgg gcaatcttca aacattgaag     1200

ataagagtgc atgagggata tgaagagttc acaatggttg ggagaagagc aacagccata     1260

ctcagaaaag caaccaggag attgattcag ctgatagtga gtgggagaga cgaacagtcg     1320

attgccgaag caataattgt ggccatggta ttttcacaag aggattgtat gataaaagca     1380

gtcagaggtg atctgaattt cgtcaatagg gcgaatcagc gattgaatcc tatgcatcaa     1440

cttttaagac attttcagaa ggatgcgaaa gtgctttttc aaaattgggg agttgaacct     1500

atcgacaatg tgatgggaat gattgggata ttgccagaca tgactccaag catcgagatg     1560

tcaatgagag gagtgagaat cagcaaaatg ggtgtagatg agtactccag cacggagagg     1620

gtagtggtga gcattgaccg ttttttgaga atccgggacc aacgaggaaa tgtactactg     1680

tctcccgagg aggtcagtga aacacaggga acagagaaac tgacaataac ttactcatcg     1740

tcaatgatgt gggagattaa tggtcctgaa tcagtgttgg tcaataccta tcaatggatc     1800

atcagaaact gggaaactgt taaaattcag tggtcccaga accctacaat gctatacaat     1860

aaaatggaat ttgaaccatt tcagtcttta gtacctaagg ccattagagg ccaatacagt     1920

gggtttgtaa gaactctgtt ccaacaaatg agggatgtgc ttgggacatt tgataccgca     1980

cagataataa aacttcttcc cttcgcagcc gctccaccaa agcaaagtag aatgcagttc     2040

tcctcattta ctgtgaatgt gaggggatca ggaatgagaa tacttgtaag gggcaattct     2100

cctgtattca actataacaa ggccacgaag agactcacag ttctcggaaa ggatgctggc     2160

actttaactg aagacccaga tgaaggcaca gctggagtgg agtccgctgt tctgagggga     2220

ttcctcattc tgggcaaaga agacaagaga tatgggccag cactaagcat caatgaactg     2280

agcaaccttg cgaaaggaga gaaggctaat gtgctaattg ggcaaggaga cgtggtacta     2340

gtgatgaaga gaaagagaga tagctctatc ttgacggatt cacaaacggc aactaagagg     2400

atccgtatgg ctattaacta ggtttaaacc ttgaacctgg gacctttgat cttggggggc     2460

tatatgaagc aattgaggag tgcctgatta atgatccctg ggttttgctt aatgcttctt     2520

ggttcaactc cttccttaca catgcattga gttagttgtg gcagtgctac tatttgctat     2580

ccatactgtc caaaaaagta ccttgtttct act                                  2613


<210>  13
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- sfGFP with PB2 packaging signals (A/Wyoming/ 03/2003)


<220>
<221>  misc_feature
<222>  (1)..(152)
<223>  PB2 5' packaging signal

<220>
<221>  misc_feature
<222>  (153)..(158)
<223>  NheI site

<220>
<221>  misc_feature
<222>  (159)..(164)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (165)..(884)
<223>  Open reading frame of sfGFP

<220>
<221>  misc_feature
<222>  (885)..(890)
<223>  XhoI site

<220>
<221>  misc_feature
<222>  (891)..(1053)
<223>  PB2 3' packaging signal

<400>  13
agcgaaagca ggtcaattat attcagtttg gaaagaataa aagaactacg gaacctgttg       60

tcgcagtctc gcactcgcga gatactgaca aaaaccacag tggaccattt ggccataatt      120

aagaagtaca catcggggag acaggaaaag aagctagcgc caccatggtc tcaaaaggag      180

aagaattatt cactggtgtg gtcccaattc ttgttgaact tgatggagac gtcaatggac      240

ataagttttc agtgagaggg gaaggtgagg gcgatgcaac taatggaaaa ttaacactca      300

aatttatttg tactactggg aagctgcccg taccatggcc cactctcgtc accacgctta      360

cttacggggt tcagtgcttc tccaggtacc ccgatcatat gaaacaacat gacttcttca      420

aatcagctat gccagaaggc tatgttcagg agagaaccat ttcattcaag gatgatggca      480

cctataagac cagagccgag gtcaagtttg aaggagatac tcttgtcaat agaattgagc      540

ttaaagggat tgattttaag gaagatggta atattcttgg ccataaattg gagtataatt      600

tcaacagcca taatgtatac atcaccgctg ataaacagaa gaatggcatt aaagcaaatt      660

ttaaaattag gcataatgtc gaggatggaa gtgttcaact tgctgaccat tatcagcaga      720

acacacccat tggagatgga ccagtccttc ttcctgataa ccattaccta tcaacccaat      780

ccgtcttgag taaagaccct aatgagaaga gagaccacat ggttctcctt gaatttgtaa      840

ctgctgctgg aattactctc ggaatggatg aactttataa atgactcgag aaaggggaaa      900

aggctaatgt gctaatcggg caaggagacg tggtgttggt aatgaaacga aaacgggact      960

ctagcatact tactgacagc cagacagcga ccaaaagaat tcggatggcc atcaattaat     1020

gttgaatagt ttaaaaacga ccttgtttct act                                  1053


<210>  14
<211>  2613
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 with PA packaging signals (A/Wyoming/03/2003)


<220>
<221>  misc_feature
<222>  (1)..(129)
<223>  PA 5' packaging signal

<220>
<221>  misc_feature
<222>  (130)..(135)
<223>  EcoRV site

<220>
<221>  misc_feature
<222>  (136)..(141)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (142)..(2421)
<223>  Open reading frame of PB2

<220>
<221>  misc_feature
<222>  (142)..(201)
<223>  Region of the PB2 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2370)..(2421)
<223>  Region of the PB2 open reading frame that contains silent 
       mutations to disrupt the naturally encoded packaging signals

<220>
<221>  misc_feature
<222>  (2422)..(2429)
<223>  PMeI site

<220>
<221>  misc_feature
<222>  (2430)..(2613)
<223>  PA 3' packaging signal

<400>  14
agcgaaagca ggtactgatt cgaattggaa gattttgtgc gacattgctt caacccgttg       60

attgtcgaac ttgcagaaaa agcattgaaa gagtttgggg aggatctgaa aattgaaaca      120

aacaaatttg atatcgccac catggaaagg atcaaagaat taagaaatct aatgagccaa      180

tccagaaccc gcgagatact gacaaaaacc acagtggacc atatggccat aattaagaag      240

tacacatcgg ggagacagga aaagaacccg tcacttagga tgaaatggat gatggcaatg      300

aaatacccaa tcactgctga caaaaggata acggaaatga ttccggagag aaatgaacaa      360

ggacaaactc tatggagtaa aatgagtgat gctggatcag atcgagtgat ggtatcacct      420

ttggctgtga catggtggaa tagaaatgga cccgtgacaa gtacggtcca ttacccaaaa      480

gtatacaaga cttattttga caaagtcgaa aggttaaaac atggaacctt tggccctgtt      540

cattttagaa atcaagtcaa gatacgccga agagtagaca taaaccctgg tcatgcggac      600

ctcagtgcca aggaggcaca agatgtaatt atggaagttg tttttcccaa tgaagtggga      660

gccaggatac taacatcaga atcgcaatta acaataacta aagagaaaaa agaagaactc      720

cgagattgca aaatttctcc cttgatggtt gcatacatgt tagagagaga acttgtccga      780

aaaacaagat ttctcccagt tgctggcgga acaagcagta tatacattga agttttacat      840

ttgactcaag ggacgtgttg ggaacaaatg tacactccag gtggagaagt gaggaatgac      900

gatgttgacc aaagcctaat tattgcagcc aggaacatag taagaagagc cgcagtatca      960

gcagatccac tagcatcttt attggagatg tgccacagca cncaaattgg cgggacaagg     1020

atggtggaca ttcttagaca gaacccgact gaagaacaag ctgtggatat atgcaaggct     1080

gcaatgggat tgagaatcag ctcatccttc agctttggtg ggtttacatt taaaagaaca     1140

agcgggtcat cagtcaaaaa agaggaagaa gtgcttacag gcaatctcca aacattgaag     1200

ataagagtac atgaggggta tgaggagttc acaatggtgg ggaaaagagc aacagctata     1260

ctcagaaaag caaccagaan nttggttcag ctcatagtga gtggaagaga cgaacagtca     1320

atagccgaag caataattgt ggccatggtg ttttcacaag aggattgcat gataaaagca     1380

gttagaggtg acctgaattt cgtcaacaga gcaaatcagc ggttgaaccc catgcatcag     1440

cttttaaggc attttcagaa agatgcgaaa gtgctttttc agaattgggg aattgagcac     1500

atcgacagtg taatgggaat ggttggagta ttaccagata tgactccaag cacagagatg     1560

tcaatgagag gaataagagt cagcaaaatg ggtgtggatg aatactccag tacagagagg     1620

gtggtggtta gcattgatcg gtttttgaga gttcgagacc aacgcgggaa tgtattatta     1680

tctcctgaag aggtcagtga aacacaggga actgagagac tgacaataac ttattcatcg     1740

tcgatgatgt gggagattaa cggtcctgag tcggttttag tcaatactta tcaatggatc     1800

atcagaaatt gggaagctgt caaaattcaa tggtctcaga atcctgcaat gttgtacaac     1860

aaaatggaat ttgaaccatt tcaatcttta gtccccaagg ccattagaag ccaatacagt     1920

gggtttgtca gaactctatt ccaacaaatg agagacgtac ttgggacatt tgacaccacc     1980

cagataataa agcttctccc ttttgcagcc gctccaccaa agcaaagcag aatgcagttc     2040

tcttcactga ctgtaaatgt gaggggatca gggatgagaa tacttgtaag gggcaattct     2100

cctgtattca actacaacaa gaccactaaa agactaacaa ttctcggaaa agatgccggc     2160

actttaattg aagacccaga tgaaagcaca tccggagtgg agtccgctgt attgagaggg     2220

tttctcatta taggtaagga agacagaaga tacgggccag cattaagcat caatgaactg     2280

agtaaccttg caaaagggga aaaggctaat gtgctaatcg ggcaaggaga cgtggtgttg     2340

gtaatgaaac gaaaacggga ctctagcata cttacagatt cacaaacagc cactaagcgt     2400

ataaggatgg ccataaacta agtttaaacc tcgaacctgg gacttttgat cttggggggc     2460

tatatgaagc aattgaggag tgcctgatta atgatccctg ggttttgctc aatgcgtctt     2520

ggttcaactc cttcctgaca catgcattaa aatagttatg gcagtgctac tatttgttat     2580

ccgtactgtc caaaaaagta ccttgtttct act                                  2613


<210>  15
<211>  2274
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB1 ORF with mutations to remove the PB1 packaging 
       signal (A/Puerto Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Mutated region

<220>
<221>  misc_feature
<222>  (2193)..(2274)
<223>  Mutated region

<400>  15
atggacgtga accctacttt gcttttcctg aaagtacctg cccagaatgc tatttccact       60

acatttccat acaccggaga ccctccttac agccatggga caggaacagg atacaccatg      120

gatactgtca acaggacaca tcagtactca gaaaagggaa gatggacaac aaacaccgaa      180

actggagcac cgcaactcaa cccgattgat gggccactgc cagaagacaa tgaaccaagt      240

ggttatgccc aaacagattg tgtattggaa gcaatggctt tccttgagga atcccatcct      300

ggtatttttg aaaactcgtg tattgaaacg atggaggttg ttcagcaaac acgagtagac      360

aagctgacac aaggccgaca gacctatgac tggactctaa atagaaacca acctgctgca      420

acagcattgg ccaacacaat agaagtgttc agatcaaatg gcctcacggc caatgagtct      480

ggaaggctca tagacttcct taaggatgta atggagtcaa tgaaaaaaga agaaatgggg      540

atcacaactc attttcagag aaagagacgg gtgagagaca atatgactaa gaaaatgata      600

acacagagaa caataggtaa aaagaagcag agattgaaca aaaggagtta tctaattaga      660

gcattgaccc tgaacacaat gaccaaagat gctgagagag ggaagctaaa acggagagca      720

attgcaaccc cagggatgca aataaggggg tttgtatact ttgttgagac actggcaagg      780

agtatatgtg agaaacttga acaatcaggg ttgccagttg gaggcaatga gaagaaagca      840

aagttggcaa atgttgtaag gaagatgatg accaattctc aggacaccga actttctttc      900

accatcactg gagataacac caaatggaac gaaaatcaga atcctcggat gtttttggcc      960

atgatcacat atatgacaag aaatcagccc gaatggttca gaaatgttct aagtattgct     1020

ccaataatgt tctcaaacaa aatggcgaga ctgggaaaag ggtatatgtt tgagagcaag     1080

agtatgaaac ttagaactca aatacctgca gaaatgctag caagcatcga tttgaaatat     1140

ttcaatgatt caacaagaaa gaagattgaa aaaatccgac cgctcttaat agaggggact     1200

gcatcattga gccctggaat gatgatgggc atgttcaata tgttaagcac tgtattaggc     1260

gtctccatcc tgaatcttgg acaaaagaga tacaccaaga ctacttactg gtgggatggt     1320

cttcaatcct ctgacgattt tgctctgatt gtgaatgcac ccaatcatga agggattcaa     1380

gccggagtcg acaggtttta tcgaacctgt aagctacttg gaatcaatat gagcaagaaa     1440

aagtcttaca taaacagaac aggtacattt gaattcacaa gttttttcta tcgttatggg     1500

tttgttgcca atttcagcat ggagctcccc agttttgggg tgtctgggat caacgagtca     1560

gcggacatga gtattggagt tactgtcatc aaaaacaata tgataaacaa tgatcttggt     1620

ccagcaacag ctcaaatggc ccttcagttg ttcatcaaag attacaggta cacgtaccga     1680

tgccatagag gtgacacaca aatacaaacc cgaagatcat ttgaaataaa gaaactgtgg     1740

gagcaaaccc gttccaaagc tggactgctg gtctccgacg gaggcccaaa tttatacaac     1800

attagaaatc tccacattcc tgaagtctgc ctaaaatggg aattgatgga tgaggattac     1860

caggggcgtt tatgcaaccc actgaaccca tttgtcagcc ataaagaaat tgaatcaatg     1920

aacaatgcag tgatgatgcc agcacatggt ccagccaaaa acatggagta tgatgctgtt     1980

gcaacaacac actcctggat ccccaaaaga aatcgatcca tcttgaatac aagtcaaaga     2040

ggagtacttg aagatgaaca aatgtaccaa aggtgctgca atttatttga aaaattcttc     2100

cccagcagtt catacagaag accagtcggg atatccagta tggtggaggc tatggtttcc     2160

agagcccgaa ttgatgcacg gattgatttc gaatcaggca gaatcaagaa ggaggaattt     2220

acagagatta tgaaaatatg ctcaaccata gaggaacttc gaaggcaaaa atag           2274


<210>  16
<211>  2280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 ORF with mutations to remove the PB2 packaging 
       signal (A/Puerto Rico/8/1934)


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Mutated region

<220>
<221>  misc_feature
<222>  (2195)..(2280)
<223>  Mutated region

<400>  16
atggagcgga tcaaggagtt gcggaacttg atgtcgcagt ctcgcacccg cgagatactc       60

acaaaaacca ccgtggacca tatggccata atcaagaagt acacatcagg aagacaggag      120

aagaacccag cacttaggat gaaatggatg atggcaatga aatatccaat tacagcagac      180

aagaggataa cggaaatgat tcctgagaga aatgagcaag gacaaacttt atggagtaaa      240

atgaatgatg caggatcaga ccgagtgatg gtatcacctc tggctgtgac atggtggaat      300

aggaatggac caataacaaa tacagttcat tatccaaaaa tctacaaaac ttattttgaa      360

agagtcgaaa ggctaaagca tggaaccttt ggccctgtcc attttagaaa ccaagtcaaa      420

atacgtcgga gagttgacat aaatcctggt catgcagatc tcagtgccaa ggaggcacag      480

gatgtaatca tggaagttgt tttccctaac gaagtgggag ccaggatact aacatcggaa      540

tcgcaactaa cgataaccaa agagaagaaa gaagaactcc aggattgcaa aatttctcct      600

ttgatggttg catacatgtt ggagagagaa ctggtccgca aaacgagatt cctcccagtg      660

gctggtggaa caagcagtgt gtacattgaa gtgttgcatt tgactcaagg aacatgctgg      720

gaacagatgt atactccagg aggggaagtg aggaatgatg atgttgatca aagcttgatt      780

attgctgcta ggaacatagt gagaagagct gcagtatcag cagatccact agcatcttta      840

ttggagatgt gccacagcac acagattggt ggaattagga tggtagacat ccttaggcag      900

aacccaacag aagagcaagc cgtggatata tgcaaggctg caatgggact gagaattagc      960

tcatccttca gttttggtgg attcacattt aagagaacaa gcggatcatc agtcaagaga     1020

gaggaagagg tgcttacggg caatcttcaa acattgaaga taagagtgca tgagggatat     1080

gaagagttca caatggttgg gagaagagca acagccatac tcagaaaagc aaccaggaga     1140

ttgattcagc tgatagtgag tgggagagac gaacagtcga ttgccgaagc aataattgtg     1200

gccatggtat tttcacaaga ggattgtatg ataaaagcag tcagaggtga tctgaatttc     1260

gtcaataggg cgaatcagcg attgaatcct atgcatcaac ttttaagaca ttttcagaag     1320

gatgcgaaag tgctttttca aaattgggga gttgaaccta tcgacaatgt gatgggaatg     1380

attgggatat tgccagacat gactccaagc atcgagatgt caatgagagg agtgagaatc     1440

agcaaaatgg gtgtagatga gtactccagc acggagaggg tagtggtgag cattgaccgt     1500

tttttgagaa tccgggacca acgaggaaat gtactactgt ctcccgagga ggtcagtgaa     1560

acacagggaa cagagaaact gacaataact tactcatcgt caatgatgtg ggagattaat     1620

ggtcctgaat cagtgttggt caatacctat caatggatca tcagaaactg ggaaactgtt     1680

aaaattcagt ggtcccagaa ccctacaatg ctatacaata aaatggaatt tgaaccattt     1740

cagtctttag tacctaaggc cattagaggc caatacagtg ggtttgtaag aactctgttc     1800

caacaaatga gggatgtgct tgggacattt gataccgcac agataataaa acttcttccc     1860

ttcgcagccg ctccaccaaa gcaaagtaga atgcagttct cctcatttac tgtgaatgtg     1920

aggggatcag gaatgagaat acttgtaagg ggcaattctc ctgtattcaa ctataacaag     1980

gccacgaaga gactcacagt tctcggaaag gatgctggca ctttaactga agacccagat     2040

gaaggcacag ctggagtgga gtccgctgtt ctgaggggat tcctcattct gggcaaagaa     2100

gacaagagat atgggccagc actaagcatc aatgaactga gcaaccttgc gaaaggagag     2160

aaggctaatg tgctaattgg gcaaggagac gtggtactag tgatgaagag aaagagagat     2220

agctctatct tgacggattc acaaacggca actaagagga tccgtatggc tattaactag     2280


<210>  17
<211>  2280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- PB2 ORF with mutations to disrupt the PB2 packaging 
       signal (A/Wyoming/03/2003)


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Mutated region

<220>
<221>  misc_feature
<222>  (861)..(861)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1139)..(1140)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2229)..(2280)
<223>  Mutated region

<400>  17
atggaaagga tcaaagaatt aagaaatcta atgagccaat ccagaacccg cgagatactg       60

acaaaaacca cagtggacca tatggccata attaagaagt acacatcggg gagacaggaa      120

aagaacccgt cacttaggat gaaatggatg atggcaatga aatacccaat cactgctgac      180

aaaaggataa cggaaatgat tccggagaga aatgaacaag gacaaactct atggagtaaa      240

atgagtgatg ctggatcaga tcgagtgatg gtatcacctt tggctgtgac atggtggaat      300

agaaatggac ccgtgacaag tacggtccat tacccaaaag tatacaagac ttattttgac      360

aaagtcgaaa ggttaaaaca tggaaccttt ggccctgttc attttagaaa tcaagtcaag      420

atacgccgaa gagtagacat aaaccctggt catgcggacc tcagtgccaa ggaggcacaa      480

gatgtaatta tggaagttgt ttttcccaat gaagtgggag ccaggatact aacatcagaa      540

tcgcaattaa caataactaa agagaaaaaa gaagaactcc gagattgcaa aatttctccc      600

ttgatggttg catacatgtt agagagagaa cttgtccgaa aaacaagatt tctcccagtt      660

gctggcggaa caagcagtat atacattgaa gttttacatt tgactcaagg gacgtgttgg      720

gaacaaatgt acactccagg tggagaagtg aggaatgacg atgttgacca aagcctaatt      780

attgcagcca ggaacatagt aagaagagcc gcagtatcag cagatccact agcatcttta      840

ttggagatgt gccacagcac ncaaattggc gggacaagga tggtggacat tcttagacag      900

aacccgactg aagaacaagc tgtggatata tgcaaggctg caatgggatt gagaatcagc      960

tcatccttca gctttggtgg gtttacattt aaaagaacaa gcgggtcatc agtcaaaaaa     1020

gaggaagaag tgcttacagg caatctccaa acattgaaga taagagtaca tgaggggtat     1080

gaggagttca caatggtggg gaaaagagca acagctatac tcagaaaagc aaccagaann     1140

ttggttcagc tcatagtgag tggaagagac gaacagtcaa tagccgaagc aataattgtg     1200

gccatggtgt tttcacaaga ggattgcatg ataaaagcag ttagaggtga cctgaatttc     1260

gtcaacagag caaatcagcg gttgaacccc atgcatcagc ttttaaggca ttttcagaaa     1320

gatgcgaaag tgctttttca gaattgggga attgagcaca tcgacagtgt aatgggaatg     1380

gttggagtat taccagatat gactccaagc acagagatgt caatgagagg aataagagtc     1440

agcaaaatgg gtgtggatga atactccagt acagagaggg tggtggttag cattgatcgg     1500

tttttgagag ttcgagacca acgcgggaat gtattattat ctcctgaaga ggtcagtgaa     1560

acacagggaa ctgagagact gacaataact tattcatcgt cgatgatgtg ggagattaac     1620

ggtcctgagt cggttttagt caatacttat caatggatca tcagaaattg ggaagctgtc     1680

aaaattcaat ggtctcagaa tcctgcaatg ttgtacaaca aaatggaatt tgaaccattt     1740

caatctttag tccccaaggc cattagaagc caatacagtg ggtttgtcag aactctattc     1800

caacaaatga gagacgtact tgggacattt gacaccaccc agataataaa gcttctccct     1860

tttgcagccg ctccaccaaa gcaaagcaga atgcagttct cttcactgac tgtaaatgtg     1920

aggggatcag ggatgagaat acttgtaagg ggcaattctc ctgtattcaa ctacaacaag     1980

accactaaaa gactaacaat tctcggaaaa gatgccggca ctttaattga agacccagat     2040

gaaagcacat ccggagtgga gtccgctgta ttgagagggt ttctcattat aggtaaggaa     2100

gacagaagat acgggccagc attaagcatc aatgaactga gtaaccttgc aaaaggggaa     2160

aaggctaatg tgctaatcgg gcaaggagac gtggtgttgg taatgaaacg aaaacgggac     2220

tctagcatac ttacagattc acaaacagcc actaagcgta taaggatggc cataaactaa     2280


