                         SEQUENCE LISTING

<110>  Westfaelische Wilhelms-Universitaet Muenster
 
<120>  GD2-UPREGULATION BY EZH2 INHIBITION IN CANCER THERAPY

<130>  UKM16557PCT

<150>  LU 101129
<151>  2019-02-14

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  1455
<212>  DNA
<213>  Artificial

<220>
<223>  GD2-BBzeta

<400>  1
atggccctgc ctgtgacagc cctgctcctg cccctggctc tcctgctgca tgccgccaga       60

cccgacatcc tgctgaccca gacacctctg agcctgcccg tgtctctggg cgaccaggcc      120

agcatcagct gccggtctag ccagagcctg gtgcaccgga acggcaacac ctacctgcac      180

tggtacctgc agaagcccgg ccagagcccc aagctgctga tccacaaggt gtccaacaga      240

ttcagcggcg tgcccgacag gttcagcggc agcggatctg gcaccgactt caccctgaag      300

atcagccggg tggaagccga ggacctgggc gtgtacttct gcagccagag cacccatgtg      360

ccccccctga ccttcggcgc tggcaccaag ctggaactga agcgggccga tgccgcccct      420

accgtgtcca tctttcctgg ctctggaggc ggaggcagcg gcggagaagt gaagctgcag      480

cagagcggcc ccagcctggt ggaacctgga gccagcgtga tgatatcctg caaggccagc      540

ggcagcagct tcaccggcta caacatgaac tgggtgcgcc agaacatcgg caagagcctg      600

gaatggatcg gcgccatcga cccctactac ggcggcacca gctacaacca gaagttcaag      660

ggcagagcca ccctgaccgt ggacaagagc agcagcaccg cctacatgca cctgaagtcc      720

ctgaccagcg aggacagcgc cgtgtactac tgtgtgtccg gcatggaata ctggggccag      780

ggaaccagcg tgaccgtgtc cagcgccaag accacccccc ccagcgtgta cggcagagtg      840

acagtgtcct cccaattgga gcccaagagc tgcgacaaga cccacacctg tccacgcgtc      900

aaattctggg tgctggtggt cgtgggcggc gtgctggcct gttatagcct gctcgtgacc      960

gtggccttca tcatcttttg ggtgcgcagc aagcggggca gaaagaagct gctgtatatc     1020

ttcaagcagc ccttcatgcg gcccgtgcag accacccagg aagaggacgg ctgctcctgc     1080

agattccccg aggaagaaga aggcggctgc gagctgagag tgaagttcag cagaagcgcc     1140

gagccccctg cctatcagca gggccagaac cagctgtata acgagctgaa cctgggcaga     1200

cgggaagagt acgacgtgct ggacaagcgg agaggcagag atcctgagat gggcggcaag     1260

cccagacgga agaaccccca ggaaggactg tataacgaac tgcagaaaga caagatggcc     1320

gaagcctaca gcgagatcgg aatgaagggc gagcggagaa gaggcaaggg ccacgatggc     1380

ctgtaccagg gcctgagcac cgccaccaag gacacctatg acgccctgca catgcaggcc     1440

ctgcccccca gataa                                                      1455


<210>  2
<211>  1446
<212>  DNA
<213>  Artificial

<220>
<223>  GD2-28zeta

<400>  2
atggccctgc ctgtgacagc cctgctcctg cccctggctc tcctgctgca tgccgccaga       60

cccgacatcc tgctgaccca gacacctctg agcctgcccg tgtctctggg cgaccaggcc      120

agcatcagct gccggtctag ccagagcctg gtgcaccgga acggcaacac ctacctgcac      180

tggtacctgc agaagcccgg ccagagcccc aagctgctga tccacaaggt gtccaacaga      240

ttcagcggcg tgcccgacag gttcagcggc agcggatctg gcaccgactt caccctgaag      300

atcagccggg tggaagccga ggacctgggc gtgtacttct gcagccagag cacccatgtg      360

ccccccctga ccttcggcgc tggcaccaag ctggaactga agcgggccga tgccgcccct      420

accgtgtcca tctttcctgg ctctggaggc ggaggcagcg gcggagaagt gaagctgcag      480

cagagcggcc ccagcctggt ggaacctgga gccagcgtga tgatatcctg caaggccagc      540

ggcagcagct tcaccggcta caacatgaac tgggtgcgcc agaacatcgg caagagcctg      600

gaatggatcg gcgccatcga cccctactac ggcggcacca gctacaacca gaagttcaag      660

ggcagagcca ccctgaccgt ggacaagagc agcagcaccg cctacatgca cctgaagtcc      720

ctgaccagcg aggacagcgc cgtgtactac tgtgtgtccg gcatggaata ctggggccag      780

ggaaccagcg tgaccgtgtc cagcgccaag accacccccc ccagcgtgta cggcagagtg      840

acagtgtcct cccaattgga gcccaagagc tgcgacaaga cccacacctg tccacgcgtc      900

aagttttggg tgctggtggt cgtgggcgga gtgctggcct gttatagcct gctcgtgaca      960

gtggccttca tcatcttttg ggtgcgcagc aagcggagca gactgctgca cagcgactac     1020

atgaacatga cccccagacg gccaggcccc acccggaaac actatcagcc ttacgcccct     1080

cccagagact tcgccgccta ccgcagcaga gtgaagttca gcagaagcgc cgagccccct     1140

gcctatcagc agggccagaa ccagctgtat aacgagctga acctgggcag acgggaagag     1200

tacgacgtgc tggataagcg gagaggccgc gaccctgaga tgggcggcaa gcctagaaga     1260

aagaaccccc aggaaggact gtataacgaa ctgcagaaag acaagatggc cgaagcctac     1320

agcgagatcg gcatgaaggg cgagcggaga agaggcaagg gccacgatgg cctgtaccag     1380

ggcctgagca ccgccaccaa ggatacctat gacgcactgc acatgcaggc tctgcctccc     1440

agatga                                                                1446


<210>  3
<211>  484
<212>  PRT
<213>  Artificial

<220>
<223>  GD2-BBzeta

<400>  3

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Leu Leu Thr Gln Thr Pro Leu Ser Leu 
            20                  25                  30          


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
        35                  40                  45              


Ser Leu Val His Arg Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
    50                  55                  60                  


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile His Lys Val Ser Asn Arg 
65                  70                  75                  80  


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
            100                 105                 110         


Phe Cys Ser Gln Ser Thr His Val Pro Pro Leu Thr Phe Gly Ala Gly 
        115                 120                 125             


Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
    130                 135                 140                 


Phe Pro Gly Ser Gly Gly Gly Gly Ser Gly Gly Glu Val Lys Leu Gln 
145                 150                 155                 160 


Gln Ser Gly Pro Ser Leu Val Glu Pro Gly Ala Ser Val Met Ile Ser 
                165                 170                 175     


Cys Lys Ala Ser Gly Ser Ser Phe Thr Gly Tyr Asn Met Asn Trp Val 
            180                 185                 190         


Arg Gln Asn Ile Gly Lys Ser Leu Glu Trp Ile Gly Ala Ile Asp Pro 
        195                 200                 205             


Tyr Tyr Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys Gly Arg Ala Thr 
    210                 215                 220                 


Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met His Leu Lys Ser 
225                 230                 235                 240 


Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Val Ser Gly Met Glu 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Lys Thr Thr 
            260                 265                 270         


Pro Pro Ser Val Tyr Gly Arg Val Thr Val Ser Ser Gln Leu Glu Pro 
        275                 280                 285             


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Arg Val Lys Phe Trp Val 
    290                 295                 300                 


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
305                 310                 315                 320 


Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Gly Arg Lys Lys 
                325                 330                 335     


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
            340                 345                 350         


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
        355                 360                 365             


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala 
    370                 375                 380                 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
385                 390                 395                 400 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                405                 410                 415     


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            420                 425                 430         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        435                 440                 445             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    450                 455                 460                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
465                 470                 475                 480 


Leu Pro Pro Arg 
                


<210>  4
<211>  481
<212>  PRT
<213>  Artificial

<220>
<223>  GD2-28zeta

<400>  4

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Leu Leu Thr Gln Thr Pro Leu Ser Leu 
            20                  25                  30          


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
        35                  40                  45              


Ser Leu Val His Arg Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
    50                  55                  60                  


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile His Lys Val Ser Asn Arg 
65                  70                  75                  80  


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
            100                 105                 110         


Phe Cys Ser Gln Ser Thr His Val Pro Pro Leu Thr Phe Gly Ala Gly 
        115                 120                 125             


Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
    130                 135                 140                 


Phe Pro Gly Ser Gly Gly Gly Gly Ser Gly Gly Glu Val Lys Leu Gln 
145                 150                 155                 160 


Gln Ser Gly Pro Ser Leu Val Glu Pro Gly Ala Ser Val Met Ile Ser 
                165                 170                 175     


Cys Lys Ala Ser Gly Ser Ser Phe Thr Gly Tyr Asn Met Asn Trp Val 
            180                 185                 190         


Arg Gln Asn Ile Gly Lys Ser Leu Glu Trp Ile Gly Ala Ile Asp Pro 
        195                 200                 205             


Tyr Tyr Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys Gly Arg Ala Thr 
    210                 215                 220                 


Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met His Leu Lys Ser 
225                 230                 235                 240 


Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Val Ser Gly Met Glu 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Lys Thr Thr 
            260                 265                 270         


Pro Pro Ser Val Tyr Gly Arg Val Thr Val Ser Ser Gln Leu Glu Pro 
        275                 280                 285             


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Arg Val Lys Phe Trp Val 
    290                 295                 300                 


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
305                 310                 315                 320 


Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
                325                 330                 335     


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
            340                 345                 350         


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
        355                 360                 365             


Ser Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln 
    370                 375                 380                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
385                 390                 395                 400 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                405                 410                 415     


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            420                 425                 430         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        435                 440                 445             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    450                 455                 460                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
465                 470                 475                 480 


Arg 
    


<210>  5
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 heavy chain CDR1

<400>  5

Asn Tyr Gly Val His 
1               5   


<210>  6
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 heavy chain CDR2

<400>  6

Val Ile Trp Ala Gly Gly Ile Thr Asn Tyr Asn Ser Ala Phe Met Ser 
1               5                   10                  15      


<210>  7
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 heavy chain CDR3

<400>  7

Arg Gly Gly His Tyr Gly Tyr Ala Leu Asp Tyr 
1               5                   10      


<210>  8
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 light chain CDR1

<400>  8

Lys Ala Ser Gln Ser Val Ser Asn Asp Val Thr 
1               5                   10      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 light chain CDR2

<400>  9

Ser Ala Ser Asn Arg Tyr Ser 
1               5           


<210>  10
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  3F8 light chain CDR3

<400>  10

Gln Gln Asp Tyr Ser Ser 
1               5       


<210>  11
<211>  746
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Gly Gln Thr Gly Lys Lys Ser Glu Lys Gly Pro Val Cys Trp Arg 
1               5                   10                  15      


Lys Arg Val Lys Ser Glu Tyr Met Arg Leu Arg Gln Leu Lys Arg Phe 
            20                  25                  30          


Arg Arg Ala Asp Glu Val Lys Ser Met Phe Ser Ser Asn Arg Gln Lys 
        35                  40                  45              


Ile Leu Glu Arg Thr Glu Ile Leu Asn Gln Glu Trp Lys Gln Arg Arg 
    50                  55                  60                  


Ile Gln Pro Val His Ile Leu Thr Ser Val Ser Ser Leu Arg Gly Thr 
65                  70                  75                  80  


Arg Glu Cys Ser Val Thr Ser Asp Leu Asp Phe Pro Thr Gln Val Ile 
                85                  90                  95      


Pro Leu Lys Thr Leu Asn Ala Val Ala Ser Val Pro Ile Met Tyr Ser 
            100                 105                 110         


Trp Ser Pro Leu Gln Gln Asn Phe Met Val Glu Asp Glu Thr Val Leu 
        115                 120                 125             


His Asn Ile Pro Tyr Met Gly Asp Glu Val Leu Asp Gln Asp Gly Thr 
    130                 135                 140                 


Phe Ile Glu Glu Leu Ile Lys Asn Tyr Asp Gly Lys Val His Gly Asp 
145                 150                 155                 160 


Arg Glu Cys Gly Phe Ile Asn Asp Glu Ile Phe Val Glu Leu Val Asn 
                165                 170                 175     


Ala Leu Gly Gln Tyr Asn Asp Asp Asp Asp Asp Asp Asp Gly Asp Asp 
            180                 185                 190         


Pro Glu Glu Arg Glu Glu Lys Gln Lys Asp Leu Glu Asp His Arg Asp 
        195                 200                 205             


Asp Lys Glu Ser Arg Pro Pro Arg Lys Phe Pro Ser Asp Lys Ile Phe 
    210                 215                 220                 


Glu Ala Ile Ser Ser Met Phe Pro Asp Lys Gly Thr Ala Glu Glu Leu 
225                 230                 235                 240 


Lys Glu Lys Tyr Lys Glu Leu Thr Glu Gln Gln Leu Pro Gly Ala Leu 
                245                 250                 255     


Pro Pro Glu Cys Thr Pro Asn Ile Asp Gly Pro Asn Ala Lys Ser Val 
            260                 265                 270         


Gln Arg Glu Gln Ser Leu His Ser Phe His Thr Leu Phe Cys Arg Arg 
        275                 280                 285             


Cys Phe Lys Tyr Asp Cys Phe Leu His Pro Phe His Ala Thr Pro Asn 
    290                 295                 300                 


Thr Tyr Lys Arg Lys Asn Thr Glu Thr Ala Leu Asp Asn Lys Pro Cys 
305                 310                 315                 320 


Gly Pro Gln Cys Tyr Gln His Leu Glu Gly Ala Lys Glu Phe Ala Ala 
                325                 330                 335     


Ala Leu Thr Ala Glu Arg Ile Lys Thr Pro Pro Lys Arg Pro Gly Gly 
            340                 345                 350         


Arg Arg Arg Gly Arg Leu Pro Asn Asn Ser Ser Arg Pro Ser Thr Pro 
        355                 360                 365             


Thr Ile Asn Val Leu Glu Ser Lys Asp Thr Asp Ser Asp Arg Glu Ala 
    370                 375                 380                 


Gly Thr Glu Thr Gly Gly Glu Asn Asn Asp Lys Glu Glu Glu Glu Lys 
385                 390                 395                 400 


Lys Asp Glu Thr Ser Ser Ser Ser Glu Ala Asn Ser Arg Cys Gln Thr 
                405                 410                 415     


Pro Ile Lys Met Lys Pro Asn Ile Glu Pro Pro Glu Asn Val Glu Trp 
            420                 425                 430         


Ser Gly Ala Glu Ala Ser Met Phe Arg Val Leu Ile Gly Thr Tyr Tyr 
        435                 440                 445             


Asp Asn Phe Cys Ala Ile Ala Arg Leu Ile Gly Thr Lys Thr Cys Arg 
    450                 455                 460                 


Gln Val Tyr Glu Phe Arg Val Lys Glu Ser Ser Ile Ile Ala Pro Ala 
465                 470                 475                 480 


Pro Ala Glu Asp Val Asp Thr Pro Pro Arg Lys Lys Lys Arg Lys His 
                485                 490                 495     


Arg Leu Trp Ala Ala His Cys Arg Lys Ile Gln Leu Lys Lys Asp Gly 
            500                 505                 510         


Ser Ser Asn His Val Tyr Asn Tyr Gln Pro Cys Asp His Pro Arg Gln 
        515                 520                 525             


Pro Cys Asp Ser Ser Cys Pro Cys Val Ile Ala Gln Asn Phe Cys Glu 
    530                 535                 540                 


Lys Phe Cys Gln Cys Ser Ser Glu Cys Gln Asn Arg Phe Pro Gly Cys 
545                 550                 555                 560 


Arg Cys Lys Ala Gln Cys Asn Thr Lys Gln Cys Pro Cys Tyr Leu Ala 
                565                 570                 575     


Val Arg Glu Cys Asp Pro Asp Leu Cys Leu Thr Cys Gly Ala Ala Asp 
            580                 585                 590         


His Trp Asp Ser Lys Asn Val Ser Cys Lys Asn Cys Ser Ile Gln Arg 
        595                 600                 605             


Gly Ser Lys Lys His Leu Leu Leu Ala Pro Ser Asp Val Ala Gly Trp 
    610                 615                 620                 


Gly Ile Phe Ile Lys Asp Pro Val Gln Lys Asn Glu Phe Ile Ser Glu 
625                 630                 635                 640 


Tyr Cys Gly Glu Ile Ile Ser Gln Asp Glu Ala Asp Arg Arg Gly Lys 
                645                 650                 655     


Val Tyr Asp Lys Tyr Met Cys Ser Phe Leu Phe Asn Leu Asn Asn Asp 
            660                 665                 670         


Phe Val Val Asp Ala Thr Arg Lys Gly Asn Lys Ile Arg Phe Ala Asn 
        675                 680                 685             


His Ser Val Asn Pro Asn Cys Tyr Ala Lys Val Met Met Val Asn Gly 
    690                 695                 700                 


Asp His Arg Ile Gly Ile Phe Ala Lys Arg Ala Ile Gln Thr Gly Glu 
705                 710                 715                 720 


Glu Leu Phe Phe Asp Tyr Arg Tyr Ser Gln Ala Asp Ala Leu Lys Tyr 
                725                 730                 735     


Val Gly Ile Glu Arg Glu Met Glu Ile Pro 
            740                 745     


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  HPRT1 forward primer

<400>  12
tgaggatttg gaaagggtgt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  HPRT1 reverse primer

<400>  13
gagcacacag agggctacaa                                                   20


<210>  14
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA used for ST8SIA1 knock-out

<400>  14
caccgccatt gaagaaatgc gcgg                                              24


<210>  15
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-H1

<400>  15

Gly Tyr Asn Met Asn 
1               5   


<210>  16
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-H2

<400>  16

Ala Ile Asp Pro Tyr Tyr Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  17
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-H3

<400>  17

Gly Met Glu Tyr 
1               


<210>  18
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-L1

<400>  18

Arg Ser Ser Gln Ser Leu Val His Arg Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  19
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-L2

<400>  19

Val Ser Asn Arg Phe Ser Gly 
1               5           


<210>  20
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a CDR-L3

<400>  20

Ser Gln Ser Thr His Val Pro Pro Leu Thr 
1               5                   10  


<210>  21
<211>  443
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a Heavy chain

<400>  21

Glu Val Gln Leu Leu Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Met Ile Ser Cys Lys Ala Ser Gly Ser Ser Phe Thr Gly Tyr 
            20                  25                  30          


Asn Met Asn Trp Val Arg Gln Asn Ile Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Asp Pro Tyr Tyr Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ser Gly Met Glu Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
            100                 105                 110         


Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
    210                 215                 220                 


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440             


<210>  22
<211>  220
<212>  PRT
<213>  Artificial

<220>
<223>  14.GD2a light chain

<400>  22

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Gln Ser Leu Val His Arg 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile His Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
        115                 120                 125             


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
145                 150                 155                 160 


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
            180                 185                 190         


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
        195                 200                 205             


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 220 


