                         SEQUENCE LISTING

<110>  Skysong Innovations
       Kong, Wei
       Fu, Lingchen
       Shi, Yixin
       Ning, Bo
 
<120>  USING TUMOR-NAVIGATING SALMONELLA TO MODULATE TUMOR METABOLISM

<130>  112624.01158

<150>  62/809,382
<151>  2019-02-22

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- ptsG deletion-insertion mutation


<220>
<221>  misc_feature
<222>  (1)..(94)
<223>  Promoter: Ptrc with lacO deletion

<400>  1
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgta gatgcgtagg       60

cacctgttac gacgaaccac acaggaaaca gaccatgttt aagaatgcat ttgctaacct      120

gcaaaaggtc ggtaaatcgc tgatgctgcc ggtatccgta ctccctatcg caggtatcct      180

gctgggtgtc ggttccgcta acttcagctg gctgccagcc gttgtatcgc acgttatggc      240

agaagcgggc ggatctgttt ttgccaatat gccgctgatt ttcgctatcg gcgtggcgtt      300

gggcttcacc aacaacgacg gcgtttcggc tctggcggcg gtagtcgcgt atggcatcat      360

ggtgaaaacc atggctgtgg ttgcgccgct ggttctgcat ttacctgctg aagagatcgc      420

cgctaaacac cttgctgata cgggtgttct cggggggatt atctccggtg caatcgcagc      480

gtatatgttt aaccgcttct accgtatcaa actgcctgag tatctgggct tctttgcggg      540

caaacgcttc gtgccgatca tttcgggtct ggccgctatt tttaccggtg tggtgctgtc      600

tttcgtatgg ccgccaatcg gtacggctat ccaggcgttc tcgcagtggg cggcttatca      660

gaacccggtt gtcgcgtttg gtatttacgg ttttatcgag cgttgcctgg tgccgtttgg      720

tctgcatcat atctggaacg ttcctttcca gatgcagatc ggcgaatata ccaacgcagc      780

gggtcaggta ttccacggcg acattccgcg ctatatggcg ggcgacccga cggcaggtat      840

gttgtctggc ggcttcctgt tcaaaatgta cggtctgccg gctgcggcca ttgctatctg      900

gcactctgct aaaccggaaa accgcgcaaa agtgggcggt atcatgattt ccgccgcgct      960

gacctcgttt ttgacgggta ttaccgagcc tatcgagttc tccttcatgt tcgtggcgcc     1020

gatcctgtac attattcacg cgattctggc aggtctggca ttcccgatct gtatcctgct     1080

ggggatgcgt gacggtacgt cgttctccca cggtctgatc gactttatcg tgctgtccgg     1140

taacagcagc aaattgtggc tgttcccgat tgttggcgcc ggttatgcga ttgtgtacta     1200

caccgtgttc cgcgtgctga tcaaagcgtt ggatctgaaa acgccgggtc gtgaagacac     1260

tactgatgac gcaaaagcgg gggcgaccag cgaaatggcg ccggctttgg ttgcggcgtt     1320

tggcggtaaa gagaacatca ctaacctgga tgcctgtatc acgcgtctgc gcgtgagcgt     1380

agccgatgta gcgaaagtgg atcaggctgg cctgaagaaa ctgggcgcgg caggcgttgt     1440

cgtcgcgggt tccggcgttc aggctatttt cggtacgaaa tccgataacc tgaaaacgga     1500

aatggatgag tacatccgta acagctaatg a                                    1531


<210>  2
<211>  6238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4960: suicide vector for construction of 
       ptsG deletion-insertion mutation

<400>  2
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caacttattg     2520

atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga     2580

gaacgacagc gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg     2640

ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc     2700

atacagcggc cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat     2760

aagacgcccc agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg     2820

gagactgtca tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca     2880

ctgttcgtcc atttccgcgc agacgatgac gtcactgccc ggctgtatgc gcgaggttac     2940

cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac gcccataatg     3000

cgggctgttg cccggcatcc aacgccattc atggccatat caatgatttt ctggtgcgta     3060

ccgggttgag aagcggtgta agtgaactgc atgaattccc gggagagctc gatatcgcat     3120

gcggtacctc tagaagaagc ttcgcggtaa agagaaccag cctgcgatgg tacgcgacgt     3180

cgcagaatat atggcggtat tgaaaggggt tgccgttgaa gaactggcgc agatcaccac     3240

cgataacttt gctcgcctgt tccatattga cgcgtctcgc ctgtcatcaa tccgttaaac     3300

gagttttttt aaagctcgta attaataaac aaaacgcgta aagttcaccg ccacaaaagg     3360

ggcggtgagc gagcttatgg aaacattcgg aaactcattt tggcagaatg tgatactttt     3420

taggctatct ggcgctgaaa cgtgatagcc gtcaaacaaa atcagacgta tttattttac     3480

tctgtgtaat aaataaaagg gcacttagat gtcctgtcca cggcggggtt ctcccccctc     3540

gccaatgcgt gagaacgtag aaaagcacaa atactcagga gcactctcaa ttattctgaa     3600

atgagctgtt gacaattaat catccggctc gtataatgtg tagatgcgta ggcacctgtt     3660

acgacgaacc acacaggaaa cagaccatgt ttaagaatgc atttgctaac ctgcaaaagg     3720

tcggtaaatc gctgatgctg ccggtatccg tactccctat cgcaggtatc ctgctgggtg     3780

tcggttccgc taacttcagc tggctgccag ccgttgtatc gcacgttatg gcagaagcgg     3840

gcggatctgt ttttgccaat atgccgctga ttttcgctat cggcgtggcg ttgggcttca     3900

ccaacaacga cggcgtttcg gctctggcgg cggtagtcgc gtatggcatc atggtgaaaa     3960

ccatggctgt ggttgcgccg ctggttctgc atttacctgc tgaagagatc gccgctaaac     4020

accttgctga tacgggtgtt ctcgggggga ttatctccgg tgcaatcgca gcgtatatgt     4080

ttaaccgctt ctaccgtatc aaactgcctg agtatctggg cttctttgcg ggcagaattc     4140

cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt     4200

atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt     4260

acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc     4320

aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct     4380

cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc     4440

tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc     4500

aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtattagggc     4560

ccgatccttt ttgtccggtg ttgggttgaa ggtgaagccg gtcggggccg cagcgggggc     4620

cggcttttca gccttgcccc cctgcttcgg ccgccgtggc tccggcgtct tgggtgccgg     4680

cgcgggttcc gcagccttgg cctgcggtgc gggcacatcg gcgggcttgg ccttgatgtg     4740

ccgcctggcg tgcgagcgga acgtctcgta ggagaacttg accttccccg tttcccgcat     4800

gtgctcccaa atggtgacga gcgcatagcc ggacgctaac gccgcctcga catccgccct     4860

caccgccagg aacgcaaccg cagcctcatc acgccggcgc ttcttggccg cgcgggattc     4920

aacccactcg gccagctcgt cggtgtagct ctttggcatc gtctctcgcc tgtcccctca     4980

gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca aaacagcagg     5040

gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt     5100

atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc     5160

ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg     5220

gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct     5280

gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac     5340

caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag     5400

agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt     5460

cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg     5520

catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc     5580

gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa     5640

gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact     5700

tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct     5760

ccatcaagaa gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga     5820

ccgagcgcct gggtcacgtg cgcgtcacga actgcgaggc aaacaccctg cccgctgtca     5880

tggccgaggt gatggcgacc cagcacggca acacccgttc cgaggccgac aagacctatc     5940

acctgctggt tagcttccgc gcgggagaga agcccgacgc ggagacgttg cgcgcgattg     6000

aggaccgcat ctgcgctggg cttggcttcg ccgagcatca gcgcgtcagt gccgtgcatc     6060

acgacaccga caacctgcac atccatatcg ccatcaacaa gattcacccg acccgaaaca     6120

ccatccatga gccgtatcgg gcctaccgcg ccctcgctga cctctgcgcg acgctcgaac     6180

gggactacgg gcttgagcgt gacaatcacg aaacgcggca gcgcgtttcc gagaaccg       6238


<210>  3
<211>  6146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4961: suicide vector for construction of 
       pykA deletion mutation

<400>  3
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caacttattg     2520

atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga     2580

gaacgacagc gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg     2640

ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc     2700

atacagcggc cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat     2760

aagacgcccc agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg     2820

gagactgtca tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca     2880

ctgttcgtcc atttccgcgc agacgatgac gtcactgccc ggctgtatgc gcgaggttac     2940

cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac gcccataatg     3000

cgggctgttg cccggcatcc aacgccattc atggccatat caatgatttt ctggtgcgta     3060

ccgggttgag aagcggtgta agtgaactgc atgaattccc gggagagctc gatatcgcat     3120

gcggtacctc tagaagaagc ttatttcgca tactggcaga accaagagcc tggtggaact     3180

ggcgcagttg gcgcgggaaa acgatgcgat ggtgatcgcc ctgacctccg ctggaacgcc     3240

gctggcgcgc gaagccacgc tggccattac cctcgacgta ccggaagata cagacattta     3300

tatgcccatg gtctctcgac ttgctcagct gaccgtgata gatgtgctgg caacgggatt     3360

tactttgcgt cgcggggcaa aattcagaga taacttgaag cgtgtcaagg aagcgctcaa     3420

ggaatcgcgt tttgataaag aattactcat caagagtgat gaccgctaaa agcaataaca     3480

atgttctacc cttttcgtca tccggggacg ttcattttat accgttaagg tttcagaacg     3540

accggatcaa tgttcacgca acaccaagtt gttttagtca acggagtatt acaactcccc     3600

cacgcctgtt catcaggtag tacagggttt gtcagcataa agcctctctt acgagaggct     3660

ttatttattt gatgggataa agatctttac gcttatacgg ctgaatctcg cctggcttgc     3720

gggttttgag cagcttcagg atccaggtgt actgttccgg atgcgggccg acaaaaattt     3780

cgacctcttc gttcatccgt ctggcgatag tgtggtcgtc agccgtgagc agatcgtcca     3840

ttggcgggcg aatctggata gtcaggcgat gcgttttacc attatacacc gggaaaagcg     3900

gtatcacgcg tgcgcggcac actttcatca gccgaccaat tgcaggcagc gtcgctttgt     3960

atgtcgcaaa gaaatcaacg aattcactat gctccgggcc gtgatcctgg tccggcaggt     4020

agtaacccca gtagccctga cggaattccg gatgagcatt catcaggcgg gcaagaatgt     4080

gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa     4140

tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat     4200

gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca     4260

ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc     4320

ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc     4380

aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga     4440

agtgatcttc cgtcacaggt attagggccc gatccttttt gtccggtgtt gggttgaagg     4500

tgaagccggt cggggccgca gcgggggccg gcttttcagc cttgcccccc tgcttcggcc     4560

gccgtggctc cggcgtcttg ggtgccggcg cgggttccgc agccttggcc tgcggtgcgg     4620

gcacatcggc gggcttggcc ttgatgtgcc gcctggcgtg cgagcggaac gtctcgtagg     4680

agaacttgac cttccccgtt tcccgcatgt gctcccaaat ggtgacgagc gcatagccgg     4740

acgctaacgc cgcctcgaca tccgccctca ccgccaggaa cgcaaccgca gcctcatcac     4800

gccggcgctt cttggccgcg cgggattcaa cccactcggc cagctcgtcg gtgtagctct     4860

ttggcatcgt ctctcgcctg tcccctcagt tcagtaattt cctgcatttg cctgtttcca     4920

gtcggtagat attccacaaa acagcaggga agcagcgctt ttccgctgca taaccctgct     4980

tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat     5040

tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga     5100

taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc     5160

tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag     5220

atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca     5280

aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca     5340

tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg     5400

actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc     5460

tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc     5520

tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg     5580

tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc     5640

gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg     5700

aagtacatca ccgacgagca aggcaagacc gagcgcctgg gtcacgtgcg cgtcacgaac     5760

tgcgaggcaa acaccctgcc cgctgtcatg gccgaggtga tggcgaccca gcacggcaac     5820

acccgttccg aggccgacaa gacctatcac ctgctggtta gcttccgcgc gggagagaag     5880

cccgacgcgg agacgttgcg cgcgattgag gaccgcatct gcgctgggct tggcttcgcc     5940

gagcatcagc gcgtcagtgc cgtgcatcac gacaccgaca acctgcacat ccatatcgcc     6000

atcaacaaga ttcacccgac ccgaaacacc atccatgagc cgtatcgggc ctaccgcgcc     6060

ctcgctgacc tctgcgcgac gctcgaacgg gactacgggc ttgagcgtga caatcacgaa     6120

acgcggcagc gcgtttccga gaaccg                                          6146


<210>  4
<211>  6146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- plasmid pK4962: suicide vector for construction of 
       PykF deletion mutation

<400>  4
cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc       60

ggctggatcg gcacgatgcg tccggcgtag aggatctgaa gatccagcag ttcaacctgt      120

tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa      180

gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg      240

tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa      300

atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa      360

ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact      420

gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga      480

catgggaatt ctgatccttt ttaacccatc acatatacct gccgttcact attatttagt      540

gaaatgagat attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc      600

aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta      660

ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca      720

gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt      780

tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca      840

ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag      900

gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa      960

catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg     1020

aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg     1080

catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa     1140

atatcaagtt cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct     1200

ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg     1260

ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta     1320

catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt     1380

ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga     1440

atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt     1500

ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc     1560

agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg     1620

aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa     1680

ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga     1740

agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata     1800

ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa     1860

aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta     1920

cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga     1980

acgcgcgaac gtctttaaaa tgaacggcaa atggtatctg ttcactgact cccgcggatc     2040

aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa     2100

ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct     2160

tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa     2220

caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac     2280

gtttgcgccc agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag     2340

catccttgaa caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata     2400

tcctattggc attttctttt atttcttatc aacataaagg tgaatcccat atgaactata     2460

taaaagcagg caaatggcta accgtattcc taaccttttg gtaatgactc caacttattg     2520

atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga     2580

gaacgacagc gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg     2640

ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc     2700

atacagcggc cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat     2760

aagacgcccc agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg     2820

gagactgtca tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca     2880

ctgttcgtcc atttccgcgc agacgatgac gtcactgccc ggctgtatgc gcgaggttac     2940

cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac gcccataatg     3000

cgggctgttg cccggcatcc aacgccattc atggccatat caatgatttt ctggtgcgta     3060

ccgggttgag aagcggtgta agtgaactgc atgaattccc gggagagctc gatatcgcat     3120

gcggtacctc tagaagaagc ttcaaccaaa acatcgccat cgacctggaa ggcaaaaagt     3180

tgctcgattg acattatgcg caacaacacc cgctgttgcg caacgccgcc cctgttcggg     3240

cggcgttatc gccagcgaca tcgtaaacag cgtaataaca aaccgttgtg gatctgcaca     3300

gatgtctttt gaaacagggt tttcattttc cttttttgta aatttcagcg tataatgcgc     3360

gccaattgtc tcttgaatgg tttcagcgca ttggactgta aaactcaacg actaaaatta     3420

tccccttccc gttgggctga aacgcgagca cacattcctc tgcacgctct ttcgatgtca     3480

cctatcctta gagcgaggca tcatcacttt agcaacacag gcttagcttc cgggccgtgc     3540

gcgcccgaag ccagatttcc atatcctcct caacttaaag actaagactg tctaattgtt     3600

gtgtgaatta atttgtttaa aaaagcgccc ttccggcgct ttttttattt aatcgatagc     3660

cactattaat aaaaaaatca aatcggattt tactatctaa ttgcggatta tctaagaaga     3720

atccgatgga agccccctgt tttctttcgg ttttttaggg actttcatgc atttcgatgc     3780

ttctttgagc gaacgatcaa aaataagtgc attcccatca aaaaaatatt ctcaacataa     3840

aaaagtttgt gtaatacttg taacgctaca tggagattaa ctcaatctag agggtattaa     3900

taatgaatcg tactaaactg gtactgggcg cggtaatcct gggttctact ctgctggctg     3960

gttgctccag caacgctaaa atcgatcagc tgtcttctga cgttcagact ctgaacgcta     4020

aagttgacca gctgagcaac gagaattccg gatgagcatt catcaggcgg gcaagaatgt     4080

gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa     4140

tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat     4200

gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca     4260

ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc     4320

ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc     4380

aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga     4440

agtgatcttc cgtcacaggt attagggccc gatccttttt gtccggtgtt gggttgaagg     4500

tgaagccggt cggggccgca gcgggggccg gcttttcagc cttgcccccc tgcttcggcc     4560

gccgtggctc cggcgtcttg ggtgccggcg cgggttccgc agccttggcc tgcggtgcgg     4620

gcacatcggc gggcttggcc ttgatgtgcc gcctggcgtg cgagcggaac gtctcgtagg     4680

agaacttgac cttccccgtt tcccgcatgt gctcccaaat ggtgacgagc gcatagccgg     4740

acgctaacgc cgcctcgaca tccgccctca ccgccaggaa cgcaaccgca gcctcatcac     4800

gccggcgctt cttggccgcg cgggattcaa cccactcggc cagctcgtcg gtgtagctct     4860

ttggcatcgt ctctcgcctg tcccctcagt tcagtaattt cctgcatttg cctgtttcca     4920

gtcggtagat attccacaaa acagcaggga agcagcgctt ttccgctgca taaccctgct     4980

tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat     5040

tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga     5100

taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc     5160

tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag     5220

atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca     5280

aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca     5340

tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg     5400

actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc     5460

tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc     5520

tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg     5580

tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc     5640

gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg     5700

aagtacatca ccgacgagca aggcaagacc gagcgcctgg gtcacgtgcg cgtcacgaac     5760

tgcgaggcaa acaccctgcc cgctgtcatg gccgaggtga tggcgaccca gcacggcaac     5820

acccgttccg aggccgacaa gacctatcac ctgctggtta gcttccgcgc gggagagaag     5880

cccgacgcgg agacgttgcg cgcgattgag gaccgcatct gcgctgggct tggcttcgcc     5940

gagcatcagc gcgtcagtgc cgtgcatcac gacaccgaca acctgcacat ccatatcgcc     6000

atcaacaaga ttcacccgac ccgaaacacc atccatgagc cgtatcgggc ctaccgcgcc     6060

ctcgctgacc tctgcgcgac gctcgaacgg gactacgggc ttgagcgtga caatcacgaa     6120

acgcggcagc gcgtttccga gaaccg                                          6146


<210>  5
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pstG Primer 1 for construction of pK4960 plasmid

<400>  5
ccaagcttcg cggtaaagag aaccagcctg cg                                     32


<210>  6
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pstG Primer 2 for construction of pK4960 plasmid

<400>  6
tggttcgtcg taacaggtgc ctacgcatct acacattata cgagccggat gattaattgt       60

caacagctca tttcagaata attgagagtg ctcct                                  95


<210>  7
<211>  119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pstG Primer 3 for construction of pK4960 plasmid

<400>  7
ctgttacgac gaaccacaca ggaaacagac catgtttaag aatgcatttg ctaacctgca       60

aaaggtcggt aaatcgctga tgctgccggt atccgtactc cctatcgcag gtatcctgc       119


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pstG Primer 4 for construction of pK4960 plasmid

<400>  8
gggaattctg cccgcaaaga agcccagata ct                                     32


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pykA Primer 1 for construction of pK4961 plasmid

<400>  9
ccaagcttat ttcgcatact ggcagaacca ag                                     32


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pykA Primer 2 for construction of pK4961 plasmid

<400>  10
gtaatactcc gttgactaaa acaa                                              24


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pykA Primer 3 for construction of pK4961 plasmid

<400>  11
aactccccca cgcctgttca tcag                                              24


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pykA Primer 4 for construction of pK4961 plasmid

<400>  12
gggaattccg tcagggctac tggggttact ac                                     32


<210>  13
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pykF Primer 1 for construction of pK4962 plasmid

<400>  13
ccaagcttca accaaaacat cgccatcgac ct                                     32


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pykF Primer 2 for construction of pK4962 plasmid

<400>  14
gacagtctta gtctttaagt tgag                                              24


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pykF Primer 3 for construction of pK4962 plasmid

<400>  15
taattgttgt gtgaattaat ttgt                                              24


<210>  16
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- pykF Primer 4 for construction of pK4962 plasmid

<400>  16
gggaattctc gttgctcagc tggtcaactt ta                                     32


