                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  RECOMBINANT BACTERIA AND USES THEREOF

<130>  10104SG1252

<150>  SG 10201901828T
<151>  2019-02-28

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DUSP10

<400>  1
atgcctccgt ctcctttaga cgacagggta gtagtggcac tatctaggcc cgtccgacct       60

caggatctca acctttgttt agactctagt taccttggct ctgccaaccc aggcagtaac      120

agccaccctc ctgtcatcgc caccaccgtt gtgtccctca aggctgcgaa tctgacgtat      180

atgccctcat ccagcggctc tgcccgctcg ctgaattgtg gatgcagcag tgccagctgc      240

tgcactgtgg caacctacga caaggacaat caggcccaaa cccaagccat tgccgctggc      300

accaccacca ctgccatcgg aacctctacc acctgccctg ctaaccagat ggtcaacaat      360

aatgagaata caggctctct aagtccatca agtggggtgg gcagccctgt gtcagggacc      420

cccaagcagc tagccagcat caaaataatc taccccaatg acttggcaaa gaagatgacc      480

aaatgcagca agagtcacct gccgagtcag ggccctgtca tcattgactg caggcccttc      540

atggagtaca acaagagtca catccaagga gctgtccaca ttaactgtgc cgataagatc      600

agccggcgga gactgcagca gggcaagatc actgtcctag acttgatttc ctgtagggaa      660

ggcaaggact ctttcaagag gatcttttcc aaagaaatta tagtttatga tgagaatacc      720

aatgaaccaa gccgagtgat gccctcccag ccacttcaca tagtcctcga gtccctgaag      780

agagaaggca aagaacctct ggtgttgaaa ggtggactta gtagttttaa gcagaaccat      840

gaaaacctct gtgacaactc cctccagctc caagagtgcc gggaggtggg gggcggcgca      900

tccgcggcct cgagcttgct acctcagccc atccccacca cccctgacat cgagaacgct      960

gagctcaccc ccatcttgcc cttcctgttc cttggcaatg agcaggatgc tcaggacctg     1020

gacaccatgc agcggctgaa catcggctac gtcatcaacg tcaccactca tcttcccctc     1080

taccactatg agaaaggcct gttcaactac aagcggctgc cagccactga cagcaacaag     1140

cagaacctgc ggcagtactt tgaagaggct tttgagttca ttgaggaagc tcaccagtgt     1200

gggaaggggc ttctcatcca ctgccaggct ggggtgtccc gctccgccac catcgtcatc     1260

gcttacttga tgaagcacac tcggatgacc atgactgatg cttataaatt tgtcaaaggc     1320

aaacgaccaa ttatctcccc aaaccttaac ttcatggggc agttgctaga gttcgaggaa     1380

gacctaaaca acggtgtgac accgagaatc cttacaccaa agctgatggg cgtggagacg     1440

gttgtgtga                                                             1449


<210>  2
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PH promoter

<400>  2
atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc       60

gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca      120

tcgggcgcg                                                              129


<210>  3
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV promoter

<400>  3
tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa       60

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata      120

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag      180

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc      240

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta      300

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg      360

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt      420

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca      480

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag      540

gtctatataa gcagagctgg tttagtgaac cgtcag                                576


<210>  4
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 promoter

<400>  4
ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt       60

atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca      120

gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta      180

actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga      240

ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag      300

tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctc                      345


<210>  5
<211>  753
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TK promoter

<400>  5
aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg       60

gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt      120

ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga      180

ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc      240

cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg      300

acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat      360

ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct      420

gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc      480

acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt      540

ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag      600

ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat      660

gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc      720

gaacaccgag cgaccctgca gcgacccgct taa                                   753


<210>  6
<211>  633
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LTR promoter

<400>  6
tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca       60

cacaaggcta cttccctgat tagcagaact acacaccagg gccagggatc agatatccac      120

tgacctttgg atggtgctac aagctagtac cagttgagcc agagaagtta gaagaagcca      180

acaaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgacccgg      240

agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag      300

agctgcatcc ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg      360

ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat      420

cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga      480

gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct      540

tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc      600

agaccctttt agtcagtgtg gaaaatctct agc                                   633


<210>  7
<211>  3280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fibronectin binding protein A

<400>  7
gcatttaaag ggagatatta tagtgaaaaa caatcttagg tacggcatta gaaaacataa       60

attgggagca gcatcagtat tcttaggaac aatgatcgtt gttgggatgg gacaagataa      120

agaagctgca gcatcagaac aaaagacaac tacagtagaa gaaaatggga attcagctac      180

tgataataaa gtaaacgaaa cacaaacaac tacaactaac gttaatacta tagatgaaac      240

acaatcatac agcgcaacag caacagaaca accgtcaaac gcaacacaag taacaactga      300

aaaagcacca aaagcagtac aagcaccaca aactgcacaa ccagcaaatg tagaaacagt      360

taaagaagag gtagttaagg aagaagcgaa ccctcaagtt aaggaaacga cacaatctca      420

agacaatagc ggagatcaaa gacaagtaga tttaacacct aaaaaggcta cacaaaatca      480

agcagcagaa acacaagttg aagtggcaca gccaagaacg gtatcagaaa gtaaaccacg      540

tgtgacaaga tcagcagatg tagcggaagc taaggaagct agtgacgcga aagtggaaac      600

gggtacagat gtgacaagta aagttacagt ggaaagtggt tctattgagg cacctcaagg      660

aaataaagta gagccacatg ctggtcaacg tgtcgtactg aaatacaaat tgaaattcga      720

aaagggttta cacaaaggag attattttga tttcaccttg tctaataatg taaatactta      780

tggagtttca acagctagaa aagtaccaga aatcaaaaat ggttcagtcg taatggcgac      840

aggtcaactt cttggaaatg ggaaaattag gtatacattt acagactata ttgattataa      900

agtaaatgtg actgctgatt tagaaatcaa tttatttatt gatcctaaaa ctgtacaaag      960

caatggacaa caaacaataa cttcaacgtt gaatgataag gaaacaaaaa acacattgcc     1020

aatagaatat aatccaggag taagtaatag ctatgctaat gtaaatggat ctattgaaac     1080

ttttgataaa gggaacaata aattcactca tgtagcttac ataaaaccac aaaatgggca     1140

taaatcagat agcgtttcaa ttactggtac actaactcaa ggtagcaaag ctaatggaaa     1200

tgttccaact gtaaaagtat atgaagtctt aaaggatgct aaagaattac cagaaagtgt     1260

atatgcaaac atatcagact ctacaatgtt taaagatgta actcaggaaa tgaaagataa     1320

attaaaagta gaaaataatg ggagttataa attagacatt gagaaattag aaaaaagtta     1380

tgttatacac tatgatggtg aatacttaag tggttcagat caagtgaatt ttagaacgca     1440

tatgtttgga tatccagaac agcagtataa gtactactat actcatttgg gataccaact     1500

tacatgggat aatggattag ttttatatag taacaaagca aaaggtgatg gaacgaatgg     1560

aacaataaca gaatcgaata atatgacatt tgatgaagaa tatggaactg gagttattac     1620

aggtcaatat gataagaatt tagtaactac tgttgaagaa gaatatgatt cttcaactct     1680

tgacattgat taccacacag ctatagatgg tgaaggtggt tatgttgatg gatacattga     1740

aacaatagaa gaaacggatt catcagctat tgatatcgat taccatactg ctgtggatag     1800

cgaagcgggt cacgttggag gatacactga gtcctctgag gaatcaaatc caattgactt     1860

tgaagaatct acgcatgaaa attcaaaaca tcacgctgat gttgttgaat atgaagagga     1920

tacaaaccca ggtggtggtc aggttactac tgagtctaac ttagttgaat ttgacgaaga     1980

gtctacaaaa ggtattgtaa ctggcgcagt gagcgaccat acaacaattg aagatacgaa     2040

agaatataca actgaaagta atctgattga actagtagat gaactacctg aagaacatgg     2100

tcaagcacaa ggaccaatcg aggaaattac tgaaaacaat catcatattt ctcattctgg     2160

tttaggaact gaaaatggtc acggtaatta tggtgtgatt gaagaaatcg aagaaaatag     2220

ccatgttgat attaagagtg aattaggtta tgaaggtggc caaaatagcg gtaaccagtc     2280

attcgaggaa gacacagaag aagataaacc taaatatgaa caaggtggca atatcgtaga     2340

tatcgatttc gatagtgtac ctcaaattca tggtcaaaat aaaggtgatc agtcattcga     2400

agaagataca gagaaagaca aacctaagta tgaacaaggt ggtaatatca ttgatatcga     2460

cttcgacagt gtgccacaaa ttcatggatt caataagcat aatgaaatta ttgaagaaga     2520

tacaaacaaa gataaaccaa attatcaatt cggtggacac aacattgttg attttgaaga     2580

agatacactt ccgaaagtaa gcggtcaaaa tgaaggtcaa caaacgattg aagaagatac     2640

aacgccgcca acgccaccga caccagaagt accgagtgag ccggaaacac caacaccacc     2700

gacgccggaa gtaccgagtg agccagaaac accaacgcca ccgacaccag aagtaccaag     2760

tgagccggaa acaccaacac cgccaacacc agaggtacca agtgagccgg aaacaccaac     2820

accgccaaca ccagaggtac cagttgaacc tggtaaacca gtaccacctg ctaaagaaga     2880

acctaaaaaa ccttctaaac cagtggaaca aggtaaagta gtaacacctg ttattgaaat     2940

caatgaaaag gttaaagcag tggcaccaac taaacaaaaa caatctaaga aatctgaact     3000

acctgaaaca ggtggagaag aatcaacaaa caaaggtatg ttgttcggcg gattattcag     3060

cattctaggt ttagcgttat tacgtagaaa taaaaagaat aacaaagcat aattaacaaa     3120

aattgacggg tttatttcat aaattacatg aagtaagcct gtttttttta tattaaatca     3180

aatttttaat agaaaattag agtgttttct gattgcttca ttggtttatg tctgatgatt     3240

gataacgaac tgagagataa agttagaatt ttaaactagt                           3280


<210>  8
<211>  559
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  erm promoter

<400>  8
cggtatcgat aagcttagtc tagaatcgat acgattttga agtggcaaca gataaaaaaa       60

agcagtttaa aattgttgct gaacttttaa aacaagcaaa tacaatcatt gtcgcaacag      120

atagcgacag agaaggcgaa aacattgcct ggtcgatcat tcataaagca aatgcctttt      180

ctaaagataa aacgtataaa agactatgga tcaatagttt agaaaaagat gtgatccgta      240

gcggttttca aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaca      300

aaaagaaaaa cgaaatgata caccaatcag tgcaaaaaaa gatataatgg gagataagac      360

ggttcgtgtt cgtgctgact tgcaccatat cataaaaatc gaaacagcaa agaatggcgg      420

aaacgtaaaa gaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt      480

gcagtatctt aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt      540

aattaagaag gagtgaatt                                                   559


<210>  9
<211>  309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nisin promoter

<400>  9
ctcctgtttt acaaccgggt gtacatagcg aaatacttgt aatgcgtggt gatgcacctg       60

aatctttctt cgaaacagat accaaatcca agctaaaatc ttttgtactc attttgagtg      120

cctccttata atttattttg tagttccttc gaacgaaatc attgtatcta acaaacttca      180

gaatttaatc agagccgttt attatgctcg cgttatcgac aataatatta ttaccaatac      240

tttctcaaga tagaattaag actgttttag atttgttaat gtttctattg tcagtatagt      300

tataagact                                                              309


<210>  10
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DUSP10

<400>  10

Met Pro Pro Ser Pro Leu Asp Asp Arg Val Val Val Ala Leu Ser Arg 
1               5                   10                  15      


Pro Val Arg Pro Gln Asp Leu Asn Leu Cys Leu Asp Ser Ser Tyr Leu 
            20                  25                  30          


Gly Ser Ala Asn Pro Gly Ser Asn Ser His Pro Pro Val Ile Ala Thr 
        35                  40                  45              


Thr Val Val Ser Leu Lys Ala Ala Asn Leu Thr Tyr Met Pro Ser Ser 
    50                  55                  60                  


Ser Gly Ser Ala Arg Ser Leu Asn Cys Gly Cys Ser Ser Ala Ser Cys 
65                  70                  75                  80  


Cys Thr Val Ala Thr Tyr Asp Lys Asp Asn Gln Ala Gln Thr Gln Ala 
                85                  90                  95      


Ile Ala Ala Gly Thr Thr Thr Thr Ala Ile Gly Thr Ser Thr Thr Cys 
            100                 105                 110         


Pro Ala Asn Gln Met Val Asn Asn Asn Glu Asn Thr Gly Ser Leu Ser 
        115                 120                 125             


Pro Ser Ser Gly Val Gly Ser Pro Val Ser Gly Thr Pro Lys Gln Leu 
    130                 135                 140                 


Ala Ser Ile Lys Ile Ile Tyr Pro Asn Asp Leu Ala Lys Lys Met Thr 
145                 150                 155                 160 


Lys Cys Ser Lys Ser His Leu Pro Ser Gln Gly Pro Val Ile Ile Asp 
                165                 170                 175     


Cys Arg Pro Phe Met Glu Tyr Asn Lys Ser His Ile Gln Gly Ala Val 
            180                 185                 190         


His Ile Asn Cys Ala Asp Lys Ile Ser Arg Arg Arg Leu Gln Gln Gly 
        195                 200                 205             


Lys Ile Thr Val Leu Asp Leu Ile Ser Cys Arg Glu Gly Lys Asp Ser 
    210                 215                 220                 


Phe Lys Arg Ile Phe Ser Lys Glu Ile Ile Val Tyr Asp Glu Asn Thr 
225                 230                 235                 240 


Asn Glu Pro Ser Arg Val Met Pro Ser Gln Pro Leu His Ile Val Leu 
                245                 250                 255     


Glu Ser Leu Lys Arg Glu Gly Lys Glu Pro Leu Val Leu Lys Gly Gly 
            260                 265                 270         


Leu Ser Ser Phe Lys Gln Asn His Glu Asn Leu Cys Asp Asn Ser Leu 
        275                 280                 285             


Gln Leu Gln Glu Cys Arg Glu Val Gly Gly Gly Ala Ser Ala Ala Ser 
    290                 295                 300                 


Ser Leu Leu Pro Gln Pro Ile Pro Thr Thr Pro Asp Ile Glu Asn Ala 
305                 310                 315                 320 


Glu Leu Thr Pro Ile Leu Pro Phe Leu Phe Leu Gly Asn Glu Gln Asp 
                325                 330                 335     


Ala Gln Asp Leu Asp Thr Met Gln Arg Leu Asn Ile Gly Tyr Val Ile 
            340                 345                 350         


Asn Val Thr Thr His Leu Pro Leu Tyr His Tyr Glu Lys Gly Leu Phe 
        355                 360                 365             


Asn Tyr Lys Arg Leu Pro Ala Thr Asp Ser Asn Lys Gln Asn Leu Arg 
    370                 375                 380                 


Gln Tyr Phe Glu Glu Ala Phe Glu Phe Ile Glu Glu Ala His Gln Cys 
385                 390                 395                 400 


Gly Lys Gly Leu Leu Ile His Cys Gln Ala Gly Val Ser Arg Ser Ala 
                405                 410                 415     


Thr Ile Val Ile Ala Tyr Leu Met Lys His Thr Arg Met Thr Met Thr 
            420                 425                 430         


Asp Ala Tyr Lys Phe Val Lys Gly Lys Arg Pro Ile Ile Ser Pro Asn 
        435                 440                 445             


Leu Asn Phe Met Gly Gln Leu Leu Glu Phe Glu Glu Asp Leu Asn Asn 
    450                 455                 460                 


Gly Val Thr Pro Arg Ile Leu Thr Pro Lys Leu Met Gly Val Glu Thr 
465                 470                 475                 480 


Val Val 
        


<210>  11
<211>  2823
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fibronectin binding protein B

<400>  11
gtgaaaagca atcttagata cggcataaga aaacacaaat tgggagcggc ctcagtattc       60

ttaggaacaa tgatcgttgt tggaatggga caagaaaaag aagctgcagc atcggaacaa      120

aacaatacta cagtagagga aagtgggagt tcagctactg aaagtaaagc aagcgaaaca      180

caaacaacta caaataacgt taatacaata gatgaaacac aatcatacag cgcgacatca      240

actgagcaac catcacaatc aacacaagta acaacagaag aagcaccgaa aactgtgcaa      300

gcaccaaaag tagaaacttc gcgagttgat ttgccatcgg aaaaagttgc tgataaggaa      360

actacaggaa ctcaagttga catagctcaa ccaagtaacg tctcagaaat taaaccaaga      420

atgaaaagat caactgacgt tacagcagtt gcagagaaag aagtagtgga agaaactaaa      480

gcgacaggta cagatgtaac aaataaagtg gaagtagaag aaggtagtga aattgtagga      540

cataaacaag atacgaatgt tgtaaatcct cataacgcag aaagagtaac cttgaaatat      600

aaatggaaat ttggagaagg aattaaggcg ggagattatt ttgatttcac attaagcgat      660

aatgttgaaa ctcatggtat ctcaacactg cgtaaagttc cggagataaa aagtacagat      720

ggtcaagtta tggcgacagg agaaataatt ggagaaagaa aagttagata tacgtttaaa      780

gaatatgtac aagaaaagaa agatttaact gctgaattat ctttaaatct atttattgat      840

cctacaacag tgacgcaaaa aggtaaccaa aatgttgaag ttaaattggg tgagactacg      900

gttagcaaaa tatttaatat tcaatattta ggtggagtta gagataattg gggagtaaca      960

gctaatggtc gaattgatac tttaaataaa gtagatggga aatttagtca ttttgcgtac     1020

atgaaaccta acaaccagtc gttaagctct gtgacagtaa ctggtcaagt aactaaagga     1080

aataaaccag gggttaataa tccaacagtt aaggtatata aacacattgg ttcagacgat     1140

ttagctgaaa gcgtatatgc aaagcttgat gatgtcagca aatttgaaga tgtgactgat     1200

aatatgagtt tagattttga tactaatggt ggttattctt taaactttaa taatttagac     1260

caaagtaaaa attatgtaat aaaatatgaa gggtattatg attcaaatgc tagcaactta     1320

gaatttcaaa cacacctttt tggatattat aactattatt atacaagtaa tttaacttgg     1380

aaaaatggcg ttgcatttta ctctaataac gctcaaggcg acggcaaaga taaactaaag     1440

gaacctatta tagaacatag tactcctatc gaacttgaat ttaaatcaga gccgccagtg     1500

gagaagcatg aattgactgg tacaatcgaa gaaagtaatg attctaagcc aattgatttt     1560

gaatatcata cagctgttga aggtgcagaa ggtcatgcag aaggtaccat tgaaactgaa     1620

gaagattcta ttcatgtaga ctttgaagaa tcgacacatg aaaattcaaa acatcatgct     1680

gatgttgttg aatatgaaga agatacaaac ccaggtggtg gtcaggttac tactgagtct     1740

aacctagttg aatttgacga agattctaca aaaggtattg taactggtgc tgttagcgat     1800

catacaacaa ttgaagatac gaaagaatat acgactgaaa gtaatctgat tgaactagta     1860

gatgaactac ctgaagaaca tggtcaagcg caaggaccaa tcgaggaaat tactgaaaac     1920

aatcatcata tttctcattc tggtttagga actgaaaatg gtcacggtaa ttatggcgtg     1980

attgaagaaa tcgaagaaaa tagccacgtg gatattaaga gtgaattagg ttacgaaggt     2040

ggccaaaata gcggtaatca gtcatttgag gaagacacag aagaagataa accgaaatat     2100

gaacaaggtg gcaatatcgt agatatcgat ttcgatagtg tacctcaaat tcatggtcaa     2160

aataatggta accaatcatt cgaagaagat acagagaaag acaaacctaa gtatgaacaa     2220

ggtggtaata tcattgatat cgacttcgac agtgtgccac atattcacgg attcaataag     2280

cacactgaaa ttattgaaga agatacaaat aaagataaac caaattatca attcggtgga     2340

cacaatagtg ttgactttga agaagataca cttccacaag taagtggtca taatgaaggt     2400

caacaaacga ttgaagaaga tacaacacct ccaatcgtgc caccaacgcc accgacacca     2460

gaagtaccaa gcgagccgga aacaccaaca ccaccgacac cagaagtacc aagcgagccg     2520

gaaacaccaa caccgccaac gccagaggta ccaactgaac ctggtaaacc aataccacct     2580

gctaaagaag aacctaaaaa accttctaaa ccagtggaac aaggtaaagt agtaacacct     2640

gttattgaaa tcaatgaaaa ggttaaagca gtggtaccaa ctaaaaaagc acaatctaag     2700

aaatctgaac tacctgaaac aggtggagaa gaatcaacaa acaacggcat gttgttcggc     2760

ggattattta gcattttagg tttagcgtta ttacgcagaa ataaaaagaa tcacaaagca     2820

taa                                                                   2823


<210>  12
<211>  1029
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fibronectin binding protein A

<400>  12

Met Lys Asn Asn Leu Arg Tyr Gly Ile Arg Lys His Lys Leu Gly Ala 
1               5                   10                  15      


Ala Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Asp 
            20                  25                  30          


Lys Glu Ala Ala Ala Ser Glu Gln Lys Thr Thr Thr Val Glu Glu Asn 
        35                  40                  45              


Gly Asn Ser Ala Thr Asp Asn Lys Val Asn Glu Thr Gln Thr Thr Thr 
    50                  55                  60                  


Thr Asn Val Asn Thr Ile Asp Glu Thr Gln Ser Tyr Ser Ala Thr Ala 
65                  70                  75                  80  


Thr Glu Gln Pro Ser Asn Ala Thr Gln Val Thr Thr Glu Lys Ala Pro 
                85                  90                  95      


Lys Ala Val Gln Ala Pro Gln Thr Ala Gln Pro Ala Asn Val Glu Thr 
            100                 105                 110         


Val Lys Glu Glu Val Val Lys Glu Glu Ala Asn Pro Gln Val Lys Glu 
        115                 120                 125             


Thr Thr Gln Ser Gln Asp Asn Ser Gly Asp Gln Arg Gln Val Asp Leu 
    130                 135                 140                 


Thr Pro Lys Lys Ala Thr Gln Asn Gln Ala Ala Glu Thr Gln Val Glu 
145                 150                 155                 160 


Val Ala Gln Pro Arg Thr Val Ser Glu Ser Lys Pro Arg Val Thr Arg 
                165                 170                 175     


Ser Ala Asp Val Ala Glu Ala Lys Glu Ala Ser Asp Ala Lys Val Glu 
            180                 185                 190         


Thr Gly Thr Asp Val Thr Ser Lys Val Thr Val Glu Ser Gly Ser Ile 
        195                 200                 205             


Glu Ala Pro Gln Gly Asn Lys Val Glu Pro His Ala Gly Gln Arg Val 
    210                 215                 220                 


Val Leu Lys Tyr Lys Leu Lys Phe Glu Lys Gly Leu His Lys Gly Asp 
225                 230                 235                 240 


Tyr Phe Asp Phe Thr Leu Ser Asn Asn Val Asn Thr Tyr Gly Val Ser 
                245                 250                 255     


Thr Ala Arg Lys Val Pro Glu Ile Lys Asn Gly Ser Val Val Met Ala 
            260                 265                 270         


Thr Gly Gln Leu Leu Gly Asn Gly Lys Ile Arg Tyr Thr Phe Thr Asp 
        275                 280                 285             


Tyr Ile Asp Tyr Lys Val Asn Val Thr Ala Asp Leu Glu Ile Asn Leu 
    290                 295                 300                 


Phe Ile Asp Pro Lys Thr Val Gln Ser Asn Gly Gln Gln Thr Ile Thr 
305                 310                 315                 320 


Ser Thr Leu Asn Asp Lys Glu Thr Lys Asn Thr Leu Pro Ile Glu Tyr 
                325                 330                 335     


Asn Pro Gly Val Ser Asn Ser Tyr Ala Asn Val Asn Gly Ser Ile Glu 
            340                 345                 350         


Thr Phe Asp Lys Gly Asn Asn Lys Phe Thr His Val Ala Tyr Ile Lys 
        355                 360                 365             


Pro Gln Asn Gly His Lys Ser Asp Ser Val Ser Ile Thr Gly Thr Leu 
    370                 375                 380                 


Thr Gln Gly Ser Lys Ala Asn Gly Asn Val Pro Thr Val Lys Val Tyr 
385                 390                 395                 400 


Glu Val Leu Lys Asp Ala Lys Glu Leu Pro Glu Ser Val Tyr Ala Asn 
                405                 410                 415     


Ile Ser Asp Ser Thr Met Phe Lys Asp Val Thr Gln Glu Met Lys Asp 
            420                 425                 430         


Lys Leu Lys Val Glu Asn Asn Gly Ser Tyr Lys Leu Asp Ile Glu Lys 
        435                 440                 445             


Leu Glu Lys Ser Tyr Val Ile His Tyr Asp Gly Glu Tyr Leu Ser Gly 
    450                 455                 460                 


Ser Asp Gln Val Asn Phe Arg Thr His Met Phe Gly Tyr Pro Glu Gln 
465                 470                 475                 480 


Gln Tyr Lys Tyr Tyr Tyr Thr His Leu Gly Tyr Gln Leu Thr Trp Asp 
                485                 490                 495     


Asn Gly Leu Val Leu Tyr Ser Asn Lys Ala Lys Gly Asp Gly Thr Asn 
            500                 505                 510         


Gly Thr Ile Thr Glu Ser Asn Asn Met Thr Phe Asp Glu Glu Tyr Gly 
        515                 520                 525             


Thr Gly Val Ile Thr Gly Gln Tyr Asp Lys Asn Leu Val Thr Thr Val 
    530                 535                 540                 


Glu Glu Glu Tyr Asp Ser Ser Thr Leu Asp Ile Asp Tyr His Thr Ala 
545                 550                 555                 560 


Ile Asp Gly Glu Gly Gly Tyr Val Asp Gly Tyr Ile Glu Thr Ile Glu 
                565                 570                 575     


Glu Thr Asp Ser Ser Ala Ile Asp Ile Asp Tyr His Thr Ala Val Asp 
            580                 585                 590         


Ser Glu Ala Gly His Val Gly Gly Tyr Thr Glu Ser Ser Glu Glu Ser 
        595                 600                 605             


Asn Pro Ile Asp Phe Glu Glu Ser Thr His Glu Asn Ser Lys His His 
    610                 615                 620                 


Ala Asp Val Val Glu Tyr Glu Glu Asp Thr Asn Pro Gly Gly Gly Gln 
625                 630                 635                 640 


Val Thr Thr Glu Ser Asn Leu Val Glu Phe Asp Glu Glu Ser Thr Lys 
                645                 650                 655     


Gly Ile Val Thr Gly Ala Val Ser Asp His Thr Thr Ile Glu Asp Thr 
            660                 665                 670         


Lys Glu Tyr Thr Thr Glu Ser Asn Leu Ile Glu Leu Val Asp Glu Leu 
        675                 680                 685             


Pro Glu Glu His Gly Gln Ala Gln Gly Pro Ile Glu Glu Ile Thr Glu 
    690                 695                 700                 


Asn Asn His His Ile Ser His Ser Gly Leu Gly Thr Glu Asn Gly His 
705                 710                 715                 720 


Gly Asn Tyr Gly Val Ile Glu Glu Ile Glu Glu Asn Ser His Val Asp 
                725                 730                 735     


Ile Lys Ser Glu Leu Gly Tyr Glu Gly Gly Gln Asn Ser Gly Asn Gln 
            740                 745                 750         


Ser Phe Glu Glu Asp Thr Glu Glu Asp Lys Pro Lys Tyr Glu Gln Gly 
        755                 760                 765             


Gly Asn Ile Val Asp Ile Asp Phe Asp Ser Val Pro Gln Ile His Gly 
    770                 775                 780                 


Gln Asn Lys Gly Asp Gln Ser Phe Glu Glu Asp Thr Glu Lys Asp Lys 
785                 790                 795                 800 


Pro Lys Tyr Glu Gln Gly Gly Asn Ile Ile Asp Ile Asp Phe Asp Ser 
                805                 810                 815     


Val Pro Gln Ile His Gly Phe Asn Lys His Asn Glu Ile Ile Glu Glu 
            820                 825                 830         


Asp Thr Asn Lys Asp Lys Pro Asn Tyr Gln Phe Gly Gly His Asn Ile 
        835                 840                 845             


Val Asp Phe Glu Glu Asp Thr Leu Pro Lys Val Ser Gly Gln Asn Glu 
    850                 855                 860                 


Gly Gln Gln Thr Ile Glu Glu Asp Thr Thr Pro Pro Thr Pro Pro Thr 
865                 870                 875                 880 


Pro Glu Val Pro Ser Glu Pro Glu Thr Pro Thr Pro Pro Thr Pro Glu 
                885                 890                 895     


Val Pro Ser Glu Pro Glu Thr Pro Thr Pro Pro Thr Pro Glu Val Pro 
            900                 905                 910         


Ser Glu Pro Glu Thr Pro Thr Pro Pro Thr Pro Glu Val Pro Ser Glu 
        915                 920                 925             


Pro Glu Thr Pro Thr Pro Pro Thr Pro Glu Val Pro Val Glu Pro Gly 
    930                 935                 940                 


Lys Pro Val Pro Pro Ala Lys Glu Glu Pro Lys Lys Pro Ser Lys Pro 
945                 950                 955                 960 


Val Glu Gln Gly Lys Val Val Thr Pro Val Ile Glu Ile Asn Glu Lys 
                965                 970                 975     


Val Lys Ala Val Ala Pro Thr Lys Gln Lys Gln Ser Lys Lys Ser Glu 
            980                 985                 990         


Leu Pro Glu Thr Gly Gly Glu Glu  Ser Thr Asn Lys Gly  Met Leu Phe 
        995                 1000                 1005             


Gly Gly  Leu Phe Ser Ile Leu  Gly Leu Ala Leu Leu  Arg Arg Asn 
    1010                 1015                 1020             


Lys Lys  Asn Asn Lys Ala 
    1025                 


<210>  13
<211>  940
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fibronectin binding protein B

<400>  13

Met Lys Ser Asn Leu Arg Tyr Gly Ile Arg Lys His Lys Leu Gly Ala 
1               5                   10                  15      


Ala Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Glu 
            20                  25                  30          


Lys Glu Ala Ala Ala Ser Glu Gln Asn Asn Thr Thr Val Glu Glu Ser 
        35                  40                  45              


Gly Ser Ser Ala Thr Glu Ser Lys Ala Ser Glu Thr Gln Thr Thr Thr 
    50                  55                  60                  


Asn Asn Val Asn Thr Ile Asp Glu Thr Gln Ser Tyr Ser Ala Thr Ser 
65                  70                  75                  80  


Thr Glu Gln Pro Ser Gln Ser Thr Gln Val Thr Thr Glu Glu Ala Pro 
                85                  90                  95      


Lys Thr Val Gln Ala Pro Lys Val Glu Thr Ser Arg Val Asp Leu Pro 
            100                 105                 110         


Ser Glu Lys Val Ala Asp Lys Glu Thr Thr Gly Thr Gln Val Asp Ile 
        115                 120                 125             


Ala Gln Pro Ser Asn Val Ser Glu Ile Lys Pro Arg Met Lys Arg Ser 
    130                 135                 140                 


Thr Asp Val Thr Ala Val Ala Glu Lys Glu Val Val Glu Glu Thr Lys 
145                 150                 155                 160 


Ala Thr Gly Thr Asp Val Thr Asn Lys Val Glu Val Glu Glu Gly Ser 
                165                 170                 175     


Glu Ile Val Gly His Lys Gln Asp Thr Asn Val Val Asn Pro His Asn 
            180                 185                 190         


Ala Glu Arg Val Thr Leu Lys Tyr Lys Trp Lys Phe Gly Glu Gly Ile 
        195                 200                 205             


Lys Ala Gly Asp Tyr Phe Asp Phe Thr Leu Ser Asp Asn Val Glu Thr 
    210                 215                 220                 


His Gly Ile Ser Thr Leu Arg Lys Val Pro Glu Ile Lys Ser Thr Asp 
225                 230                 235                 240 


Gly Gln Val Met Ala Thr Gly Glu Ile Ile Gly Glu Arg Lys Val Arg 
                245                 250                 255     


Tyr Thr Phe Lys Glu Tyr Val Gln Glu Lys Lys Asp Leu Thr Ala Glu 
            260                 265                 270         


Leu Ser Leu Asn Leu Phe Ile Asp Pro Thr Thr Val Thr Gln Lys Gly 
        275                 280                 285             


Asn Gln Asn Val Glu Val Lys Leu Gly Glu Thr Thr Val Ser Lys Ile 
    290                 295                 300                 


Phe Asn Ile Gln Tyr Leu Gly Gly Val Arg Asp Asn Trp Gly Val Thr 
305                 310                 315                 320 


Ala Asn Gly Arg Ile Asp Thr Leu Asn Lys Val Asp Gly Lys Phe Ser 
                325                 330                 335     


His Phe Ala Tyr Met Lys Pro Asn Asn Gln Ser Leu Ser Ser Val Thr 
            340                 345                 350         


Val Thr Gly Gln Val Thr Lys Gly Asn Lys Pro Gly Val Asn Asn Pro 
        355                 360                 365             


Thr Val Lys Val Tyr Lys His Ile Gly Ser Asp Asp Leu Ala Glu Ser 
    370                 375                 380                 


Val Tyr Ala Lys Leu Asp Asp Val Ser Lys Phe Glu Asp Val Thr Asp 
385                 390                 395                 400 


Asn Met Ser Leu Asp Phe Asp Thr Asn Gly Gly Tyr Ser Leu Asn Phe 
                405                 410                 415     


Asn Asn Leu Asp Gln Ser Lys Asn Tyr Val Ile Lys Tyr Glu Gly Tyr 
            420                 425                 430         


Tyr Asp Ser Asn Ala Ser Asn Leu Glu Phe Gln Thr His Leu Phe Gly 
        435                 440                 445             


Tyr Tyr Asn Tyr Tyr Tyr Thr Ser Asn Leu Thr Trp Lys Asn Gly Val 
    450                 455                 460                 


Ala Phe Tyr Ser Asn Asn Ala Gln Gly Asp Gly Lys Asp Lys Leu Lys 
465                 470                 475                 480 


Glu Pro Ile Ile Glu His Ser Thr Pro Ile Glu Leu Glu Phe Lys Ser 
                485                 490                 495     


Glu Pro Pro Val Glu Lys His Glu Leu Thr Gly Thr Ile Glu Glu Ser 
            500                 505                 510         


Asn Asp Ser Lys Pro Ile Asp Phe Glu Tyr His Thr Ala Val Glu Gly 
        515                 520                 525             


Ala Glu Gly His Ala Glu Gly Thr Ile Glu Thr Glu Glu Asp Ser Ile 
    530                 535                 540                 


His Val Asp Phe Glu Glu Ser Thr His Glu Asn Ser Lys His His Ala 
545                 550                 555                 560 


Asp Val Val Glu Tyr Glu Glu Asp Thr Asn Pro Gly Gly Gly Gln Val 
                565                 570                 575     


Thr Thr Glu Ser Asn Leu Val Glu Phe Asp Glu Asp Ser Thr Lys Gly 
            580                 585                 590         


Ile Val Thr Gly Ala Val Ser Asp His Thr Thr Ile Glu Asp Thr Lys 
        595                 600                 605             


Glu Tyr Thr Thr Glu Ser Asn Leu Ile Glu Leu Val Asp Glu Leu Pro 
    610                 615                 620                 


Glu Glu His Gly Gln Ala Gln Gly Pro Ile Glu Glu Ile Thr Glu Asn 
625                 630                 635                 640 


Asn His His Ile Ser His Ser Gly Leu Gly Thr Glu Asn Gly His Gly 
                645                 650                 655     


Asn Tyr Gly Val Ile Glu Glu Ile Glu Glu Asn Ser His Val Asp Ile 
            660                 665                 670         


Lys Ser Glu Leu Gly Tyr Glu Gly Gly Gln Asn Ser Gly Asn Gln Ser 
        675                 680                 685             


Phe Glu Glu Asp Thr Glu Glu Asp Lys Pro Lys Tyr Glu Gln Gly Gly 
    690                 695                 700                 


Asn Ile Val Asp Ile Asp Phe Asp Ser Val Pro Gln Ile His Gly Gln 
705                 710                 715                 720 


Asn Asn Gly Asn Gln Ser Phe Glu Glu Asp Thr Glu Lys Asp Lys Pro 
                725                 730                 735     


Lys Tyr Glu Gln Gly Gly Asn Ile Ile Asp Ile Asp Phe Asp Ser Val 
            740                 745                 750         


Pro His Ile His Gly Phe Asn Lys His Thr Glu Ile Ile Glu Glu Asp 
        755                 760                 765             


Thr Asn Lys Asp Lys Pro Asn Tyr Gln Phe Gly Gly His Asn Ser Val 
    770                 775                 780                 


Asp Phe Glu Glu Asp Thr Leu Pro Gln Val Ser Gly His Asn Glu Gly 
785                 790                 795                 800 


Gln Gln Thr Ile Glu Glu Asp Thr Thr Pro Pro Ile Val Pro Pro Thr 
                805                 810                 815     


Pro Pro Thr Pro Glu Val Pro Ser Glu Pro Glu Thr Pro Thr Pro Pro 
            820                 825                 830         


Thr Pro Glu Val Pro Ser Glu Pro Glu Thr Pro Thr Pro Pro Thr Pro 
        835                 840                 845             


Glu Val Pro Thr Glu Pro Gly Lys Pro Ile Pro Pro Ala Lys Glu Glu 
    850                 855                 860                 


Pro Lys Lys Pro Ser Lys Pro Val Glu Gln Gly Lys Val Val Thr Pro 
865                 870                 875                 880 


Val Ile Glu Ile Asn Glu Lys Val Lys Ala Val Val Pro Thr Lys Lys 
                885                 890                 895     


Ala Gln Ser Lys Lys Ser Glu Leu Pro Glu Thr Gly Gly Glu Glu Ser 
            900                 905                 910         


Thr Asn Asn Gly Met Leu Phe Gly Gly Leu Phe Ser Ile Leu Gly Leu 
        915                 920                 925             


Ala Leu Leu Arg Arg Asn Lys Lys Asn His Lys Ala 
    930                 935                 940 


