                         SEQUENCE LISTING

<110>  UNIVERSITY OF VIRGINIA PATENT FOUNDATION
       AI, HUI-WANG
       YEH, HSIEN-WEI
       XIONG, YING
 
<120>  ATP-INDEPENDENT BIOLUMINESCENT REPORTER VARIANTS TO IMPROVE IN 
       VIVO IMAGING

<130>  3062-109 PCT

<150>  US 62/811,129
<151>  2019-02-27

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  1

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170     


<210>  2
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  2

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Ser Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asn His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
                165                 170     


<210>  3
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  3

Lys Val Phe Thr Leu Gly Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Gln Ala Gln Val Leu Glu Gln Gly Gly Leu Thr Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Cys Asp Gln Met Ala Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Ala Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Gln Pro Tyr Glu Gly Ile Ala Lys Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Thr Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
                165                 170     


<210>  4
<211>  1185
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  4
atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag       60

ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag      120

ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac      180

atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc      240

cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc      300

gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc      360

tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag      420

acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc      480

gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc      540

acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg      600

gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc      660

cgccactcca ccggaaagac tctcggggat tttgttgggg actggcgaca gacagccggc      720

tacaaccagg ctcaagtcct tgaacaggga ggtttgacca gtttgtttca gaacctcggg      780

gtgtccgtaa ctccaatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgat      840

atccatgtca tcatcccgta tgaaggtctg agctgcgacc aaatggccca gatcgaaaaa      900

atttttaagg tggtataccc tgtggatgat catcacttta aggcgatcct gcactatggc      960

acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggaca gccgtatgaa     1020

ggcatcgcca agttcgacgg caaaaagatc acagtaacag ggaccctgtg gaacggcaac     1080

acaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatt     1140

aacggagtga ccggctggcg tctgcatgaa cgcattctgg cgtaa                     1185


<210>  5
<211>  394
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  5

Met Val Ser Lys Gly Glu Ala Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      


Val His Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              


Val Thr Lys Gly Gly Pro Leu Pro Phe Ser Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  


Gln Phe Met Tyr Gly Ser Arg Ala Phe Ile Lys His Pro Ala Asp Ile 
65                  70                  75                  80  


Pro Asp Tyr Tyr Lys Gln Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      


Val Met Asn Phe Glu Asp Gly Gly Ala Val Thr Val Thr Gln Asp Thr 
            100                 105                 110         


Ser Leu Glu Asp Gly Thr Leu Ile Tyr Lys Val Lys Leu Arg Gly Thr 
        115                 120                 125             


Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 


Glu Ala Ser Thr Glu Arg Leu Tyr Pro Glu Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Ile Lys Met Ala Leu Arg Leu Lys Asp Gly Gly Arg Tyr Leu Ala 
                165                 170                 175     


Asp Phe Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Met Pro Gly 
            180                 185                 190         


Ala Tyr Asn Val Asp Arg Lys Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             


Tyr Thr Val Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg His Ser Thr 
    210                 215                 220                 


Gly Lys Thr Leu Gly Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly 
225                 230                 235                 240 


Tyr Asn Gln Ala Gln Val Leu Glu Gln Gly Gly Leu Thr Ser Leu Phe 
                245                 250                 255     


Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser 
            260                 265                 270         


Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu 
        275                 280                 285             


Gly Leu Ser Cys Asp Gln Met Ala Gln Ile Glu Lys Ile Phe Lys Val 
    290                 295                 300                 


Val Tyr Pro Val Asp Asp His His Phe Lys Ala Ile Leu His Tyr Gly 
305                 310                 315                 320 


Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly 
                325                 330                 335     


Gln Pro Tyr Glu Gly Ile Ala Lys Phe Asp Gly Lys Lys Ile Thr Val 
            340                 345                 350         


Thr Gly Thr Leu Trp Asn Gly Asn Thr Ile Ile Asp Glu Arg Leu Ile 
        355                 360                 365             


Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr 
    370                 375                 380                 


Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
385                 390                 


<210>  6
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  6

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Gln Ala Lys Ala Leu Glu Gln Gly Gly Leu Thr Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Cys Asp Gln Met Ala Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Gln Pro Tyr Glu Gly Ile Ala Lys Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
                165                 170     


<210>  7
<211>  1179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  7
atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag       60

ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag      120

ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac      180

atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc      240

cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc      300

gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc      360

tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag      420

acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc      480

gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc      540

acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg      600

gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc      660

cgccatcttg atacactcga agatttcgtt ggggactggc gacagacagc cggctacaac      720

ttgagtcaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc      780

gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat      840

gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt      900

aaggtggtgt accctgtgga taatcatcac tttaaggtga tcctgcacta tggcacactg      960

gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc     1020

gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt     1080

atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga     1140

gtgaccggct ggcgtctgca tgaacgcatt ctggcgtaa                            1179


<210>  8
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  8

Met Val Ser Lys Gly Glu Ala Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      


Val His Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              


Val Thr Lys Gly Gly Pro Leu Pro Phe Ser Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  


Gln Phe Met Tyr Gly Ser Arg Ala Phe Ile Lys His Pro Ala Asp Ile 
65                  70                  75                  80  


Pro Asp Tyr Tyr Lys Gln Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      


Val Met Asn Phe Glu Asp Gly Gly Ala Val Thr Val Thr Gln Asp Thr 
            100                 105                 110         


Ser Leu Glu Asp Gly Thr Leu Ile Tyr Lys Val Lys Leu Arg Gly Thr 
        115                 120                 125             


Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 


Glu Ala Ser Thr Glu Arg Leu Tyr Pro Glu Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Ile Lys Met Ala Leu Arg Leu Lys Asp Gly Gly Arg Tyr Leu Ala 
                165                 170                 175     


Asp Phe Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Met Pro Gly 
            180                 185                 190         


Ala Tyr Asn Val Asp Arg Lys Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             


Tyr Thr Val Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg His Leu Asp 
    210                 215                 220                 


Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr Asn 
225                 230                 235                 240 


Leu Ser Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln Asn 
                245                 250                 255     


Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly Glu 
            260                 265                 270         


Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu 
        275                 280                 285             


Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val Tyr 
    290                 295                 300                 


Pro Val Asp Asn His His Phe Lys Val Ile Leu His Tyr Gly Thr Leu 
305                 310                 315                 320 


Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg Pro 
                325                 330                 335     


Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr Gly 
            340                 345                 350         


Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn Pro 
        355                 360                 365             


Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly Trp 
    370                 375                 380                 


Arg Leu His Glu Arg Ile Leu Ala 
385                 390         


<210>  9
<211>  1962
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  9
atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag       60

ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag      120

ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac      180

atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc      240

cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc      300

gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc      360

tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag      420

acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc      480

gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc      540

acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaggttg      600

gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc      660

cgcaagcgtc aagatgctag cgaagaagag ctatcagagt gctttcgcgt ttttgacaag      720

gatggaaatg gtttcatcga cagggaggaa tttggagaca tcatccgcct tactggagaa      780

cagctcacgg atgaggatcc cgatgagata tttggagatt cagacacaga caaaaatgga      840

aggattgact ttgatgagtt cctgaagatg gtggagaatg tccagtatgt taatattctc      900

aaagtcttca ctctcgggga ttttgttggg gactggcgac agacagccgg ctacaaccag      960

gctcaagtcc ttgaacaggg aggtttgacc agtttgtttc agaacctcgg ggtgtccgta     1020

actccaatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga tatccatgtc     1080

atcatcccgt atgaaggtct gagctgcgac caaatggccc agatcgaaaa aatttttaag     1140

gtggtatacc ctgtggatga tcatcacttt aaggcgatcc tgcactatgg cacactggta     1200

atcgacgggg ttacgccgaa catgatcgac tatttcggac agccgtatga aggcatcgcc     1260

aagttcgacg gcaaaaagat cacagtaaca gggaccctga taatgcatga ccaactgaca     1320

gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc     1380

accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa     1440

gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt     1500

cctgaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc     1560

cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctcagtta     1620

cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata     1680

agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg     1740

acagcaaagg ggggcgggtc caagaggcgc tggaagaaaa acttcattgc cgtcagcgct     1800

gccaaccggt tcaagaagat ctccagctcc ggggcactgg agctttggaa cggcaacaca     1860

attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccattaac     1920

ggagtgaccg gctggcgtct gcatgaacgc attctggcgt aa                        1962


<210>  10
<211>  653
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  10

Met Val Ser Lys Gly Glu Ala Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      


Val His Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              


Val Thr Lys Gly Gly Pro Leu Pro Phe Ser Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  


Gln Phe Met Tyr Gly Ser Arg Ala Phe Ile Lys His Pro Ala Asp Ile 
65                  70                  75                  80  


Pro Asp Tyr Tyr Lys Gln Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      


Val Met Asn Phe Glu Asp Gly Gly Ala Val Thr Val Thr Gln Asp Thr 
            100                 105                 110         


Ser Leu Glu Asp Gly Thr Leu Ile Tyr Lys Val Lys Leu Arg Gly Thr 
        115                 120                 125             


Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 


Glu Ala Ser Thr Glu Arg Leu Tyr Pro Glu Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Ile Lys Met Ala Leu Arg Leu Lys Asp Gly Gly Arg Tyr Leu Ala 
                165                 170                 175     


Asp Phe Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Met Pro Gly 
            180                 185                 190         


Ala Tyr Asn Val Asp Arg Arg Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             


Tyr Thr Val Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg Lys Arg Gln 
    210                 215                 220                 


Asp Ala Ser Glu Glu Glu Leu Ser Glu Cys Phe Arg Val Phe Asp Lys 
225                 230                 235                 240 


Asp Gly Asn Gly Phe Ile Asp Arg Glu Glu Phe Gly Asp Ile Ile Arg 
                245                 250                 255     


Leu Thr Gly Glu Gln Leu Thr Asp Glu Asp Pro Asp Glu Ile Phe Gly 
            260                 265                 270         


Asp Ser Asp Thr Asp Lys Asn Gly Arg Ile Asp Phe Asp Glu Phe Leu 
        275                 280                 285             


Lys Met Val Glu Asn Val Gln Tyr Val Asn Ile Leu Lys Val Phe Thr 
    290                 295                 300                 


Leu Gly Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr Asn Gln 
305                 310                 315                 320 


Ala Gln Val Leu Glu Gln Gly Gly Leu Thr Ser Leu Phe Gln Asn Leu 
                325                 330                 335     


Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly Glu Asn 
            340                 345                 350         


Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu Ser 
        355                 360                 365             


Cys Asp Gln Met Ala Gln Ile Glu Lys Ile Phe Lys Val Val Tyr Pro 
    370                 375                 380                 


Val Asp Asp His His Phe Lys Ala Ile Leu His Tyr Gly Thr Leu Val 
385                 390                 395                 400 


Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Gln Pro Tyr 
                405                 410                 415     


Glu Gly Ile Ala Lys Phe Asp Gly Lys Lys Ile Thr Val Thr Gly Thr 
            420                 425                 430         


Leu Ile Met His Asp Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys 
        435                 440                 445             


Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
    450                 455                 460                 


Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
465                 470                 475                 480 


Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
                485                 490                 495     


Thr Ile Tyr Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
            500                 505                 510         


Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
        515                 520                 525             


Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Gln Leu Arg His Val Met 
    530                 535                 540                 


Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
545                 550                 555                 560 


Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
                565                 570                 575     


Val Gln Met Met Thr Ala Lys Gly Gly Gly Ser Lys Arg Arg Trp Lys 
            580                 585                 590         


Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser 
        595                 600                 605             


Ser Ser Gly Ala Leu Glu Leu Trp Asn Gly Asn Thr Ile Ile Asp Glu 
    610                 615                 620                 


Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
625                 630                 635                 640 


Gly Val Thr Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
                645                 650             


<210>  11
<211>  1962
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  11
atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag       60

ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag      120

ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac      180

atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc      240

cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc      300

gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc      360

tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag      420

acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc      480

gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc      540

acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaggttg      600

gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc      660

cgcaagcgtc aagatgctag cgaagaagag ctatcagagt gctttcgcgt ttttgacaag      720

gatggaaatg gtttcatcga cagggaggaa tttggagaca tcatccgcct tactggagaa      780

cagctcacgg atgaggatcc cgatgagata tttggagatt cagacacaga caaaaatgga      840

aggattgact ttgatgagtt cctgaagatg gtggagaatg tccagtatgt taatattctc      900

atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacttg      960

agtcaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta     1020

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc     1080

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag     1140

gtggtgtacc ctgtggataa tcatcacttt aaggtgatcc tgcactatgg cacactggta     1200

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc     1260

gtgttcgacg gcaaaaagat cactgtaaca gggaccctga taatgcatga ccaactgaca     1320

gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc     1380

accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa     1440

gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt     1500

cctgaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc     1560

cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctcagtta     1620

cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata     1680

agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg     1740

acagcaaagg ggggcgggtc caagaggcgc tggaagaaaa acttcattgc cgtcagcgct     1800

gccaaccggt tcaagaagat ctccagctcc ggggcactgg agctttggaa cggcaacaaa     1860

attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac     1920

ggagtgaccg gctggcgtct gcatgaacgc attctggcgt aa                        1962


<210>  12
<211>  653
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  12

Met Val Ser Lys Gly Glu Ala Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      


Val His Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              


Val Thr Lys Gly Gly Pro Leu Pro Phe Ser Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  


Gln Phe Met Tyr Gly Ser Arg Ala Phe Ile Lys His Pro Ala Asp Ile 
65                  70                  75                  80  


Pro Asp Tyr Tyr Lys Gln Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      


Val Met Asn Phe Glu Asp Gly Gly Ala Val Thr Val Thr Gln Asp Thr 
            100                 105                 110         


Ser Leu Glu Asp Gly Thr Leu Ile Tyr Lys Val Lys Leu Arg Gly Thr 
        115                 120                 125             


Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 


Glu Ala Ser Thr Glu Arg Leu Tyr Pro Glu Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Ile Lys Met Ala Leu Arg Leu Lys Asp Gly Gly Arg Tyr Leu Ala 
                165                 170                 175     


Asp Phe Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Met Pro Gly 
            180                 185                 190         


Ala Tyr Asn Val Asp Arg Arg Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             


Tyr Thr Val Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg Lys Arg Gln 
    210                 215                 220                 


Asp Ala Ser Glu Glu Glu Leu Ser Glu Cys Phe Arg Val Phe Asp Lys 
225                 230                 235                 240 


Asp Gly Asn Gly Phe Ile Asp Arg Glu Glu Phe Gly Asp Ile Ile Arg 
                245                 250                 255     


Leu Thr Gly Glu Gln Leu Thr Asp Glu Asp Pro Asp Glu Ile Phe Gly 
            260                 265                 270         


Asp Ser Asp Thr Asp Lys Asn Gly Arg Ile Asp Phe Asp Glu Phe Leu 
        275                 280                 285             


Lys Met Val Glu Asn Val Gln Tyr Val Asn Ile Leu Met Val Phe Thr 
    290                 295                 300                 


Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr Asn Leu 
305                 310                 315                 320 


Ser Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln Asn Leu 
                325                 330                 335     


Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly Glu Asn 
            340                 345                 350         


Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu Ser 
        355                 360                 365             


Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val Tyr Pro 
    370                 375                 380                 


Val Asp Asn His His Phe Lys Val Ile Leu His Tyr Gly Thr Leu Val 
385                 390                 395                 400 


Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg Pro Tyr 
                405                 410                 415     


Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr Gly Thr 
            420                 425                 430         


Leu Ile Met His Asp Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys 
        435                 440                 445             


Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
    450                 455                 460                 


Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
465                 470                 475                 480 


Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
                485                 490                 495     


Thr Ile Tyr Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
            500                 505                 510         


Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
        515                 520                 525             


Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Gln Leu Arg His Val Met 
    530                 535                 540                 


Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
545                 550                 555                 560 


Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
                565                 570                 575     


Val Gln Met Met Thr Ala Lys Gly Gly Gly Ser Lys Arg Arg Trp Lys 
            580                 585                 590         


Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser 
        595                 600                 605             


Ser Ser Gly Ala Leu Glu Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu 
    610                 615                 620                 


Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
625                 630                 635                 640 


Gly Val Thr Gly Trp Arg Leu His Glu Arg Ile Leu Ala 
                645                 650             


<210>  13
<211>  936
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  13
atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg       60

tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag      120

aagcacgccg agaacgccgt gatttttctg catggtaacg ctacctccag ctacctgtgg      180

aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga      240

atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac      300

ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac      360

tggggggctg ctctggcctt tcactacgcc tacgagcacc aagacaggat caaggccatc      420

gtccatatgg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag      480

gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc      540

ttcgtcgaga ccgtgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct      600

gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct      660

cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac      720

aacgcctacc ttcgggccag cgacgatctg cctaagctgt tcatcgagtc cgaccctggg      780

ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag      840

gtgaagggcc tccacttcct ccaggaggac gctccagatg aaatgggtaa gtacatcaag      900

agcttcgtgg agcgcgtgct gaagaacgag cagtaa                                936


<210>  14
<211>  311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  14

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      


Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          


Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              


Phe Leu His Gly Asn Ala Thr Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  


Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  


Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      


His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         


Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Ala Leu Ala Phe His 
        115                 120                 125             


Tyr Ala Tyr Glu His Gln Asp Arg Ile Lys Ala Ile Val His Met Glu 
    130                 135                 140                 


Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 


Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     


Glu Asn Asn Phe Phe Val Glu Thr Val Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         


Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             


Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 


Leu Val Lys Gly Gly Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr 
225                 230                 235                 240 


Asn Ala Tyr Leu Arg Ala Ser Asp Asp Leu Pro Lys Leu Phe Ile Glu 
                245                 250                 255     


Ser Asp Pro Gly Phe Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys 
            260                 265                 270         


Phe Pro Asn Thr Glu Phe Val Lys Val Lys Gly Leu His Phe Leu Gln 
        275                 280                 285             


Glu Asp Ala Pro Asp Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu 
    290                 295                 300                 


Arg Val Leu Lys Asn Glu Gln 
305                 310     


<210>  15
<211>  1653
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase oligonucleotide

<400>  15
atggaagatg ccaaaaacat taagaagggc ccagcgccgt tctacccact cgaagacggg       60

accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcgccatc      120

gcctttaccg acgcacatat tcaggtggac gttacctacg ccgagtactt cgagatgagc      180

gttcggctgg cagaagctat gaggcgctat gggctgaata caaaccatcg gatcgtggtg      240

tgcagcgaga atagctcgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg      300

gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc      360

agccagccca ccgtcgtatt cgtgagcaag aaagggctgc gaaaggtcct caacgtgcaa      420

aagaagctac cgatcatacg aaagatcatc atcatggata gcaagaccga ctaccagggc      480

ttccaaagca tgtacacctt cgtgacttcc catttgccac ccagcttcaa cgagtacgac      540

ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggt      600

agtacaggat tacccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt      660

catgcccgcg accccatctt cggctaccag aacatccccg acaccgctat cctcagcgtg      720

gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt      780

cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat      840

aagattcaat ctgccctgct ggtgcccaca ctatttagct gcctcgctaa gagcactctc      900

atcgacaagt acgacctaag cagcttgcgc gagatcgcca gcggcggggc gccgctcagc      960

aaggaggtag gtgaggccgt ggccaaacgc ttccgcctac caggcatccg ccagggctat     1020

ggcctgacag aaacaaccaa cgccgtcatg atcacccccg agggggaccg taagcctggc     1080

tcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tagacttggt caccggtaag     1140

acactgggtg tgaaccagcg cggtgagctg tgcgtccgtg gccccatgat catgagcggc     1200

tacgttaaca accccgaggc tacgaacgct ctcatcgaca aggacggctg gctgcacagc     1260

ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc     1320

ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagggcat cctgctgcaa     1380

cacccctaca tcttcgacgc cggagtcgcc ggcctgcccg acgacgatgc cggcgagctg     1440

cccgccgcag tcgtcgtgtt ggaacacggt aaaaccatga ccgagaaaga gatcgtggac     1500

tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gtttgtggat     1560

gaagtcccta gaggatcgac cggcaagtta gacgcccgca agatccgcga gattctcact     1620

aaggccaaga aggacggcaa gatcgccgtg taa                                  1653


<210>  16
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  16

Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      


Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          


Tyr Ala Leu Val Pro Gly Ala Ile Ala Phe Thr Asp Ala His Ile Gln 
        35                  40                  45              


Val Asp Val Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  


Glu Ala Met Arg Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  


Cys Ser Glu Asn Ser Ser Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      


Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         


Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             


Ser Lys Lys Gly Leu Arg Lys Val Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 


Ile Ile Arg Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 


Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Ser Phe 
                165                 170                 175     


Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         


Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             


Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 


Pro Ile Phe Gly Tyr Gln Asn Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 


Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     


Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         


Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             


Pro Thr Leu Phe Ser Cys Leu Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 


Asp Leu Ser Ser Leu Arg Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 


Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe Arg Leu Pro Gly Ile 
                325                 330                 335     


Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Asn Ala Val Met Ile Thr 
            340                 345                 350         


Pro Glu Gly Asp Arg Lys Pro Gly Ser Val Gly Lys Val Val Pro Phe 
        355                 360                 365             


Phe Glu Ala Lys Val Val Asp Leu Val Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 


Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 


Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     


Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         


Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             


Val Ala Pro Ala Glu Leu Glu Gly Ile Leu Leu Gln His Pro Tyr Ile 
    450                 455                 460                 


Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 


Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     


Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         


Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Arg Gly Ser Thr Gly 
        515                 520                 525             


Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Thr Lys Ala Lys Lys 
    530                 535                 540                 


Asp Gly Lys Ile Ala Val 
545                 550 


<210>  17
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  17

Glu Phe Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170     


<210>  18
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially mutated luciferase polypeptide

<400>  18

Met Val Leu Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Asp 
1               5                   10                  15      


Gly Tyr Asn Gln Ala Gln Val Leu Glu Gln Gly Gly Leu Thr Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Arg Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Gln Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Arg 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Ser Glu Arg Ile Leu Ala 
                165                 170     


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  19
atgccatagc atttttatcc                                                   20


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  20
gatttaatct gtatcagg                                                     18


<210>  21
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  21
taccgagctc atccagtttg gactagtgg                                         29


<210>  22
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  22
agcccagatc tcctctagag tctagatctg g                                      31


<210>  23
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  23
aaagccaccg gcgcgccgcc gccaccatgg tcttcactct cgaagatttt gt               52


<210>  24
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  24
tcgaagcggc cggccttacg ccagaatgcg ttcatgca                               38


<210>  25
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  25
aaagccaccg gcgcgccgcc gccaccatgg aagatgccaa aaacattaag a                51


<210>  26
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  26
tcgaagcggc cggccttaca cggcgatctt gccgtccttc tt                          42


<210>  27
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  27
aaagccaccg gcgcgccgcc gccaccatgg cttccaaggt gtacgacc                    48


<210>  28
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  28
tcgaagcggc cggccttact gctcgttctt cagcacgcgc t                           41


<210>  29
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, t or u

<400>  29
cagacagccg gctacaacnn knnkcaagtc cttgaacagg gaggtgtg                    48


<210>  30
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, t or u

<400>  30
cacacctccc tgttcaagga cttgmnnmnn gttgtagccg gctgtctg                    48


<210>  31
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, t or u

<400>  31
caagtccttg aacagggagg tnnknnknnk ttgtttcaga atctcggggt g                51


<210>  32
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, t or u

<400>  32
caccccgaga ttctgaaaca amnnmnnmnn acctccctgt tcaaggactt g                51


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  33
atgccatagc atttttatcc                                                   20


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  34
gatttaatct gtatcagg                                                     18


<210>  35
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  35
aataaagctt gccgccacca tggtcttcac tctcggggat ttt                         43


<210>  36
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  36
taattctcga gttacgccag aatgcgttca tgcag                                  35


<210>  37
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  37
attataaagc ttgccgccac catggaagat gccaaaaaca ttaaga                      46


<210>  38
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  38
ataactcgag catggaagat gccaaaaaca ttaaga                                 36


<210>  39
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  39
ttgccaagct tacacggcga tcttgccgtc cttctt                                 36


<210>  40
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  40
attataaagc ttgccgccac catggtgagc aagggcgagg cagt                        44


<210>  41
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  41
ataactcgag catggtgagc aagggcgagg cagtg                                  35


<210>  42
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  42
ttgccaagct tacgccagaa tgcgttcatg ca                                     32


<210>  43
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, t or u

<400>  43
gagggccgcc actccaccgg annkactctc ggggattttg ttggg                       45


<210>  44
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, t or u

<400>  44
cccaacaaaa tccccgagag tmnntccggt ggagtggcgg ccctc                       45


<210>  45
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial oligonucleotide promoter

<400>  45
ttattctcga gttacacggc gatcttgccg tccttctt                               38


