                         SEQUENCE LISTING

<110>  Cancer Research Technology Limited
 
<120>  NGS Sequencing

<130>  P35908WO/SNB

<150>  GB1903391.9
<151>  12.03.2019

<160>  75    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter


<220>
<221>  T7 RNA polymerase promoter
<222>  (1)..(20)

<400>  1
attatgctga gtgatatccc                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse T7 RNA polymerase promoter


<220>
<221>  Reverse T7 RNA polymerase promoter
<222>  (1)..(20)

<400>  2
taatacgact cactataggg                                                   20


<210>  3
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1


<220>
<221>  T7dT_U1_Lcf1
<222>  (1)..(72)
<223>  "n" is a, t, g, or c

<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  6x "n" = Molecular Bar Code

<400>  3
ggccggtaat cactagctcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  4
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1 Reverse


<220>
<221>  T7dT_U1_Lcf1 Reverse
<222>  (1)..(72)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (48)..(53)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (58)..(63)
<223>  6x "n" = Molecular Bar Code

<400>  4
tccggccatt atgctgagtg atatccccgt gctgcgagaa ggctagannn nnnctcgatc       60

actaatggcc gg                                                           72


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U2P_Lig1


<220>
<221>  U2P_Lig1
<222>  (1)..(20)

<400>  5
agatcggaag agcacacgtc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U2P_RT_1


<220>
<221>  U2P_RT_1
<222>  (1)..(20)

<400>  6
gacgtgtgct cttccgatct                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U2P_RT_1 Reversed


<220>
<221>  U2P_RT_1 Reversed
<222>  (1)..(20)

<400>  7
tctagccttc tcgtgtgcag                                                   20


<210>  8
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7_U1_Hsp92II02c3


<220>
<221>  T7_U1_Hsp92II02c3
<222>  (1)..(75)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (21)..(26)
<223>  6x "n" = Molecular Bar Code

<400>  8
aggccggtaa tcactagctc nnnnnnagat cggaagagcg tcgtgcccct atagtgagtc       60

gtattaccgg cctca                                                        75


<210>  9
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7_U1_Hsp92II03c3


<220>
<221>  T7_U1_Hsp92II03c3
<222>  (1)..(76)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (21)..(26)
<223>  6x "n" = Molecular Bar Code

<400>  9
aggccggtaa tcactagctc nnnnnnagat cggaagagcg tcgtgcccct atagtgagtc       60

gtattaccgg cctcat                                                       76


<210>  10
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illumina Universal


<220>
<221>  Illumina Universal
<222>  (1)..(58)

<400>  10
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  11
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illumina Index (1)


<220>
<221>  Illumina Index (1)
<222>  (1)..(64)

<400>  11
caagcagaag acggcatacg agatcgtgat gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  12
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REVERSE OF Illumina Index (1)


<220>
<221>  REVERSE OF Illumina Index (1)
<222>  (1)..(64)

<400>  12
tctagccttc tcgtgtgcag acttgaggtc agtgtagtgc tagagcatac ggcagaagac       60

gaac                                                                    64


<210>  13
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1


<220>
<221>  T7dT_U1_Lcf1
<222>  (1)..(72)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  6x "n" = Molecular Bar Code

<400>  13
ggccggtaat cactagctcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  14
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1 Reverse


<220>
<221>  T7dT_U1_Lcf1 Reverse
<222>  (1)..(72)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (48)..(53)
<223>  6x "n" = Molecular Bar Code

<400>  14
tccggccatt atgctgagtg atatccccgt gctgcgagaa ggctagannn nnnctcgatc       60

actaatggcc gg                                                           72


<210>  15
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1	cfDNA Ligation Product


<220>
<221>  T7dT_U1_Lcf1 cfDNA Ligation Product
<222>  (1)..(73)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (48)..(53)
<223>  6x "n" = Molecular Bar Code

<400>  15
tccggccatt atgctgagtg atatccccgt gctgcgagaa ggctagannn nnnctcgatc       60

actaatggcc gga                                                          73


<210>  16
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7U1_Prim1


<220>
<221>  T7U1_Prim1
<222>  (1)..(46)

<400>  16
ggccggtaat acgactcact ataggggcac gacgctcttc cgatct                      46


<210>  17
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1 cfDNA Predicted aRNA


<220>
<221>  T7dT_U1_Lcf1 cfDNA Predicted aRNA
<222>  (1)..(49)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  6x "n" = Molecular Bar Code

<400>  17
ggggcacgac gctcttccga tctnnnnnng agctagtgat taccggcct                   49


<210>  18
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aRNA Ligation Product


<220>
<221>  aRNA Ligation Product
<222>  (1)..(89)
<223>  "n" is a, t, g, or c

<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  6x "n" = Molecular Bar Code

<220>
<221>  misc_feature
<222>  (50)..(69)
<223>  Target gene sequence

<400>  18
ggggcacgac gctcttccga tctnnnnnng agctagtgat taccggcctn nnnnnnnnnn       60

nnnnnnnnna gatcggaaga gcacacgtc                                         89


<210>  19
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ligated aRNA 1st RT Product


<220>
<221>  Ligated aRNA 1st RT Product
<222>  (1)..(89)
<223>  "n" is a, t, c, or g

<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  6x "n" = Molecular Bar Code

<220>
<221>  misc_feature
<222>  (50)..(69)
<223>  Target gene sequence

<400>  19
ccccgtgctg cgagaaggct agannnnnnc tcgatcacta atggccggan nnnnnnnnnn       60

nnnnnnnnnt ctagccttct cgtgtgcag                                         89


<210>  20
<211>  124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NGS PCR 1st Cycle on RT Product


<220>
<221>  NGS PCR 1st Cycle on RT Product
<222>  (1)..(124)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (59)..(64)
<223>  6x "n" = Molecular Bar Code

<220>
<221>  misc_feature
<222>  (85)..(104)
<223>  Target gene sequence

<400>  20
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnngagcta gtgattaccg gcctnnnnnn nnnnnnnnnn nnnnagatcg gaagagcaca      120

cgtc                                                                   124


<210>  21
<211>  167
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NGS PCR 2nd Cycle on 1st Cycle Product


<220>
<221>  NGS PCR 2nd Cycle on 1st Cycle Product
<222>  (1)..(167)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (59)..(64)
<223>  6x "n" = Molecular Bar Code

<220>
<221>  misc_feature
<222>  (85)..(103)
<223>  Target gene sequence

<400>  21
ttactatgcc gctggtggct ctagatgtga gaaagggatg tgctgcgaga aggctagann       60

nnnnctcgat cactaatggc cggannnnnn nnnnnnnnnn nnntctagcc ttctcgtgtg      120

cagacttgag gtcagtgtag tgctagagca tacggcagaa gacgaac                    167


<210>  22
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NGS PCR 2nd Cycle on 1st Cycle Product Reverse


<220>
<221>  Reverse NGS PCR 2nd Cycle on 1st Cycle Product
<222>  (1)..(168)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (59)..(64)
<223>  6x "n" = Molecular Bar Code

<220>
<221>  misc_feature
<222>  (85)..(104)
<223>  Target gene sequence

<400>  22
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnngagcta gtgattaccg gcctnnnnnn nnnnnnnnnn nnnnagatcg gaagagcaca      120

cgtctgaact ccagtcacat cacgatctcg tatgccgtct tctgcttg                   168


<210>  23
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7_U1_Hsp92II03c3


<220>
<221>  T7_U1_Hsp92II03c3
<222>  (1)..(76)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (21)..(26)
<223>  6x "n" = Molecular Bar Code

<400>  23
aggccggtaa tcactagctc nnnnnnagat cggaagagcg tcgtgcccct atagtgagtc       60

gtattaccgg cctcat                                                       76


<210>  24
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7_U1_ Hsp92II3c3 Ligation Product Linearised


<220>
<221>  T7_U1_ Hsp92II3c3 Ligation Product Linearised
<222>  (1)..(80)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (51)..(56)
<223>  6x "n" = Molecular Bar Code

<400>  24
tactccggcc attatgctga gtgatatccc cgtgctgcga gaaggctaga nnnnnnctcg       60

atcactaatg gccggagtac                                                   80


<210>  25
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7_U1_ Hsp92II3c3 Ligation Product Predicted aRNA


<220>
<221>  T7_U1_ Hsp92II3c3 Ligation Product Predicted aRNA
<222>  (1)..(53)
<223>  "n" is a, c, t, or g

<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  6x "n" = Molecular Bar Code

<400>  25
ggggcacgac gctcttccga tctnnnnnng agctagtgat taccggcctc atg              53


<210>  26
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf1


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  26
ggccggtaat cactagctcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  27
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf2


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  27
ggccggtaaa accaaccagn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  28
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf3


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  28
ggccggtaaa accaagaccn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  29
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf4


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  29
ggccggtaaa accaatggan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  30
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf5


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  30
ggccggtaaa atccatccgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  31
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf6


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  31
ggccggtaaa atcctattan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  32
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf7


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  32
ggccggtaaa atcggtagan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  33
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf8


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  33
ggccggtaaa gaacggtcgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  34
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf9


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  34
ggccggtaaa gaactcaggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  35
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf10


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  35
ggccggtaaa gaagaccgcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  36
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf11


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  36
ggccggtaaa tacgaatacn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  37
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf12


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  37
ggccggtaaa tactcaacgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  38
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf13


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  38
ggccggtaaa tacttggccn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  39
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf14


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  39
ggccggtaac aggaggttan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  40
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf15


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  40
ggccggtaac aggccatccn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  41
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf16


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  41
ggccggtaac aggcgctggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  42
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf17


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  42
ggccggtaac cttacgttcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  43
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf18


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  43
ggccggtaac cttagttggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  44
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf19


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  44
ggccggtaac cttatactgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  45
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf20


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  45
ggccggtaac tccagaggcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  46
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf21


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  46
ggccggtaac tcctaccaan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  47
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf22


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  47
ggccggtaac tcgaatcggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  48
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf23


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  48
ggccggtaag ccaatacggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  49
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf24


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  49
ggccggtaag ccagccatgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  50
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf25


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  50
ggccggtaag ccagttctan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  51
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf26


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  51
ggccggtaag tatagttgcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  52
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf27


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  52
ggccggtaag tatcctacgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  53
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf28


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  53
ggccggtaag tcaaggtaan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  54
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf29


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  54
ggccggtaat ctgccattcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  55
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf30


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  55
ggccggtaat ctggtatagn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  56
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf31


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  56
ggccggtaat ctggtcgtcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  57
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf32


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  57
ggccggtaaa atggcaagcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  58
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf33


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  58
ggccggtaaa ccaagcctcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  59
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf34


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  59
ggccggtaaa ccagtaatgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  60
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf35


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  60
ggccggtaac taatggctcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  61
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf36


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  61
ggccggtaac tatcttatcn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  62
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf37


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  62
ggccggtaac tcaatggcgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  63
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf38


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  63
ggccggtaaa accagaaggn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  64
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf39


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  64
ggccggtaaa accaggctan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  65
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf40


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  65
ggccggtaaa accagttacn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  66
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf41


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  66
ggccggtaaa atctccgccn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  67
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf42


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  67
ggccggtaaa atgaatcaan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  68
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf43


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  68
ggccggtaaa atgagagtan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  69
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf44


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  69
ggccggtaaa gaagcgatgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  70
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf45


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  70
ggccggtaaa gaagtatgan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  71
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf46


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  71
ggccggtaaa gaataattgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  72
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf47


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  72
ggccggtaaa tagaattgan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  73
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf48


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  73
ggccggtaaa tagaccttgn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  74
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf49


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  74
ggccggtaaa tagagagccn nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


<210>  75
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7dT_U1_Lcf50


<220>
<221>  misc_feature
<222>  (20)..(25)
<223>  n is a, c, g, or t

<400>  75
ggccggtaac aggtaataan nnnnnagatc ggaagagcgt cgtgccccta tagtgagtcg       60

tattaccggc ct                                                           72


