                         SEQUENCE LISTING

<110>  University of Puerto Rico
 
<120>  ARTIFICIAL FUSION OF DEHYDRATASE ENZYMES TO IMPROVE PRODUCTION OF
        FATTY ACIDS

<130>  19-184-WO

<140>  PCT/US20/021077
<141>  2020-03-05

<150>  62/815,676
<151>  2019-03-08

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E.Coli and P. Profundum

<400>  1

Met Val Asp Lys Arg Glu Ser Tyr Thr Lys Glu Asp Leu Leu Ala Ser 
1               5                   10                  15      


Gly Arg Gly Glu Leu Phe Gly Ala Lys Gly Pro Gln Leu Pro Ala Pro 
            20                  25                  30          


Asn Met Leu Met Met Asp Arg Val Val Lys Met Thr Glu Thr Gly Gly 
        35                  40                  45              


Asn Phe Asp Lys Gly Tyr Val Glu Ala Glu Leu Asp Ile Asn Pro Asp 
    50                  55                  60                  


Leu Trp Phe Phe Gly Cys His Phe Ile Gly Asp Pro Val Met Pro Gly 
65                  70                  75                  80  


Cys Leu Gly Leu Asp Ala Met Trp Gln Leu Val Gly Phe Tyr Leu Gly 
                85                  90                  95      


Trp Leu Gly Gly Glu Gly Lys Gly Arg Ala Leu Gly Val Gly Glu Val 
            100                 105                 110         


Lys Phe Thr Gly Gln Val Leu Pro Thr Ala Lys Lys Val Thr Tyr Arg 
        115                 120                 125             


Ile His Phe Lys Arg Ile Val Asn Arg Arg Leu Ile Met Gly Leu Ala 
    130                 135                 140                 


Asp Gly Glu Val Leu Val Asp Gly Arg Leu Ile Tyr Thr Ala Ser Asp 
145                 150                 155                 160 


Leu Lys Val Gly Leu Phe Gln Asp Thr Ser Ala Phe Ser Thr Gln Asn 
                165                 170                 175     


Val Ala Ile Gln Thr Ala Ala Pro Val Ala Ser Ala Ser Asn Gly Leu 
            180                 185                 190         


Asp Ala Ala Gln Val Gln Gly Thr Met Val Asp Lys Arg Glu Ser Tyr 
        195                 200                 205             


Thr Lys Glu Asp Leu Leu Ala Ser Gly Arg Gly Glu Leu Phe Gly Ala 
    210                 215                 220                 


Lys Gly Pro Gln Leu Pro Ala Pro Asn Met Leu Met Met Asp Arg Val 
225                 230                 235                 240 


Val Lys Met Thr Glu Thr Gly Gly Asn Phe Asp Lys Gly Tyr Val Glu 
                245                 250                 255     


Ala Glu Leu Asp Ile Asn Pro Asp Leu Trp Phe Phe Gly Cys His Phe 
            260                 265                 270         


Ile Gly Asp Pro Val Met Pro Gly Cys Leu Gly Leu Asp Ala Met Trp 
        275                 280                 285             


Gln Leu Val Gly Phe Tyr Leu Gly Trp Leu Gly Gly Glu Gly Lys Gly 
    290                 295                 300                 


Arg Ala Leu Gly Val Gly Glu Val Lys Phe Thr Gly Gln Val Leu Pro 
305                 310                 315                 320 


Thr Ala Lys Lys Val Thr Tyr Arg Ile His Phe Lys Arg Ile Val Asn 
                325                 330                 335     


Arg Arg Leu Ile Met Gly Leu Ala Asp Gly Glu Val Leu Val Asp Gly 
            340                 345                 350         


Arg Leu Ile Tyr Thr Ala Ser Asp Leu Lys Val Gly Leu Phe Gln Asp 
        355                 360                 365             


Thr Ser Ala Phe 
    370         


<210>  2
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E.Coli and P. Profundum

<400>  2

Met Thr Thr Asn Thr His Thr Leu Gln Ile Glu Glu Ile Leu Glu Leu 
1               5                   10                  15      


Leu Pro His Arg Phe Pro Phe Leu Leu Val Asp Arg Val Leu Asp Phe 
            20                  25                  30          


Glu Glu Gly Arg Phe Leu Arg Ala Val Lys Asn Val Ser Val Asn Glu 
        35                  40                  45              


Pro Phe Phe Gln Gly His Phe Pro Gly Lys Pro Ile Phe Pro Gly Val 
    50                  55                  60                  


Leu Ile Leu Glu Ala Met Ala Gln Ala Thr Gly Ile Leu Ala Phe Lys 
65                  70                  75                  80  


Ser Val Gly Lys Leu Glu Pro Gly Glu Leu Tyr Tyr Phe Ala Gly Ile 
                85                  90                  95      


Asp Glu Ala Arg Phe Lys Arg Pro Val Val Pro Gly Asp Gln Met Ile 
            100                 105                 110         


Met Glu Val Thr Phe Glu Lys Thr Arg Arg Gly Leu Thr Arg Phe Lys 
        115                 120                 125             


Gly Val Ala Leu Val Asp Gly Lys Val Val Cys Glu Ala Thr Met Met 
    130                 135                 140                 


Cys Ala Arg Ser Arg Glu Ala Ser Thr Gln Asn Val Ala Ile Gln Thr 
145                 150                 155                 160 


Ala Ala Pro Val Ala Ser Ala Ser Asn Gly Leu Asp Ala Ala Gln Val 
                165                 170                 175     


Gln Gly Thr Met Thr Thr Asn Thr His Thr Leu Gln Ile Glu Glu Ile 
            180                 185                 190         


Leu Glu Leu Leu Pro His Arg Phe Pro Phe Leu Leu Val Asp Arg Val 
        195                 200                 205             


Leu Asp Phe Glu Glu Gly Arg Phe Leu Arg Ala Val Lys Asn Val Ser 
    210                 215                 220                 


Val Asn Glu Pro Phe Phe Gln Gly His Phe Pro Gly Lys Pro Ile Phe 
225                 230                 235                 240 


Pro Gly Val Leu Ile Leu Glu Ala Met Ala Gln Ala Thr Gly Ile Leu 
                245                 250                 255     


Ala Phe Lys Ser Val Gly Lys Leu Glu Pro Gly Glu Leu Tyr Tyr Phe 
            260                 265                 270         


Ala Gly Ile Asp Glu Ala Arg Phe Lys Arg Pro Val Val Pro Gly Asp 
        275                 280                 285             


Gln Met Ile Met Glu Val Thr Phe Glu Lys Thr Arg Arg Gly Leu Thr 
    290                 295                 300                 


Arg Phe Lys Gly Val Ala Leu Val Asp Gly Lys Val Val Cys Glu Ala 
305                 310                 315                 320 


Thr Met Met Cys Ala Arg Ser Arg Glu Ala 
                325                 330 


<210>  3
<211>  351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E.Coli and P. Profundum

<400>  3

Met Val Asp Lys Arg Glu Ser Tyr Thr Lys Glu Asp Leu Leu Ala Ser 
1               5                   10                  15      


Gly Arg Gly Glu Leu Phe Gly Ala Lys Gly Pro Gln Leu Pro Ala Pro 
            20                  25                  30          


Asn Met Leu Met Met Asp Arg Val Val Lys Met Thr Glu Thr Gly Gly 
        35                  40                  45              


Asn Phe Asp Lys Gly Tyr Val Glu Ala Glu Leu Asp Ile Asn Pro Asp 
    50                  55                  60                  


Leu Trp Phe Phe Gly Cys His Phe Ile Gly Asp Pro Val Met Pro Gly 
65                  70                  75                  80  


Cys Leu Gly Leu Asp Ala Met Trp Gln Leu Val Gly Phe Tyr Leu Gly 
                85                  90                  95      


Trp Leu Gly Gly Glu Gly Lys Gly Arg Ala Leu Gly Val Gly Glu Val 
            100                 105                 110         


Lys Phe Thr Gly Gln Val Leu Pro Thr Ala Lys Lys Val Thr Tyr Arg 
        115                 120                 125             


Ile His Phe Lys Arg Ile Val Asn Arg Arg Leu Ile Met Gly Leu Ala 
    130                 135                 140                 


Asp Gly Glu Val Leu Val Asp Gly Arg Leu Ile Tyr Thr Ala Ser Asp 
145                 150                 155                 160 


Leu Lys Val Gly Leu Phe Gln Asp Thr Ser Ala Phe Ser Thr Gln Asn 
                165                 170                 175     


Val Ala Ile Gln Thr Ala Ala Pro Val Ala Ser Ala Ser Asn Gly Leu 
            180                 185                 190         


Asp Ala Ala Gln Val Gln Gly Thr Met Thr Thr Asn Thr His Thr Leu 
        195                 200                 205             


Gln Ile Glu Glu Ile Leu Glu Leu Leu Pro His Arg Phe Pro Phe Leu 
    210                 215                 220                 


Leu Val Asp Arg Val Leu Asp Phe Glu Glu Gly Arg Phe Leu Arg Ala 
225                 230                 235                 240 


Val Lys Asn Val Ser Val Asn Glu Pro Phe Phe Gln Gly His Phe Pro 
                245                 250                 255     


Gly Lys Pro Ile Phe Pro Gly Val Leu Ile Leu Glu Ala Met Ala Gln 
            260                 265                 270         


Ala Thr Gly Ile Leu Ala Phe Lys Ser Val Gly Lys Leu Glu Pro Gly 
        275                 280                 285             


Glu Leu Tyr Tyr Phe Ala Gly Ile Asp Glu Ala Arg Phe Lys Arg Pro 
    290                 295                 300                 


Val Val Pro Gly Asp Gln Met Ile Met Glu Val Thr Phe Glu Lys Thr 
305                 310                 315                 320 


Arg Arg Gly Leu Thr Arg Phe Lys Gly Val Ala Leu Val Asp Gly Lys 
                325                 330                 335     


Val Val Cys Glu Ala Thr Met Met Cys Ala Arg Ser Arg Glu Ala 
            340                 345                 350     


<210>  4
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E.Coli and P. Profundum

<400>  4

Met Thr Thr Asn Thr His Thr Leu Gln Ile Glu Glu Ile Leu Glu Leu 
1               5                   10                  15      


Leu Pro His Arg Phe Pro Phe Leu Leu Val Asp Arg Val Leu Asp Phe 
            20                  25                  30          


Glu Glu Gly Arg Phe Leu Arg Ala Val Lys Asn Val Ser Val Asn Glu 
        35                  40                  45              


Pro Phe Phe Gln Gly His Phe Pro Gly Lys Pro Ile Phe Pro Gly Val 
    50                  55                  60                  


Leu Ile Leu Glu Ala Met Ala Gln Ala Thr Gly Ile Leu Ala Phe Lys 
65                  70                  75                  80  


Ser Val Gly Lys Leu Glu Pro Gly Glu Leu Tyr Tyr Phe Ala Gly Ile 
                85                  90                  95      


Asp Glu Ala Arg Phe Lys Arg Pro Val Val Pro Gly Asp Gln Met Ile 
            100                 105                 110         


Met Glu Val Thr Phe Glu Lys Thr Arg Arg Gly Leu Thr Arg Phe Lys 
        115                 120                 125             


Gly Val Ala Leu Val Asp Gly Lys Val Val Cys Glu Ala Thr Met Met 
    130                 135                 140                 


Cys Ala Arg Ser Arg Glu Ala Ser Thr Gln Asn Val Ala Ile Gln Thr 
145                 150                 155                 160 


Ala Ala Pro Val Ala Ser Ala Ser Asn Gly Leu Asp Ala Ala Gln Val 
                165                 170                 175     


Gln Gly Thr Met Val Asp Lys Arg Glu Ser Tyr Thr Lys Glu Asp Leu 
            180                 185                 190         


Leu Ala Ser Gly Arg Gly Glu Leu Phe Gly Ala Lys Gly Pro Gln Leu 
        195                 200                 205             


Pro Ala Pro Asn Met Leu Met Met Asp Arg Val Val Lys Met Thr Glu 
    210                 215                 220                 


Thr Gly Gly Asn Phe Asp Lys Gly Tyr Val Glu Ala Glu Leu Asp Ile 
225                 230                 235                 240 


Asn Pro Asp Leu Trp Phe Phe Gly Cys His Phe Ile Gly Asp Pro Val 
                245                 250                 255     


Met Pro Gly Cys Leu Gly Leu Asp Ala Met Trp Gln Leu Val Gly Phe 
            260                 265                 270         


Tyr Leu Gly Trp Leu Gly Gly Glu Gly Lys Gly Arg Ala Leu Gly Val 
        275                 280                 285             


Gly Glu Val Lys Phe Thr Gly Gln Val Leu Pro Thr Ala Lys Lys Val 
    290                 295                 300                 


Thr Tyr Arg Ile His Phe Lys Arg Ile Val Asn Arg Arg Leu Ile Met 
305                 310                 315                 320 


Gly Leu Ala Asp Gly Glu Val Leu Val Asp Gly Arg Leu Ile Tyr Thr 
                325                 330                 335     


Ala Ser Asp Leu Lys Val Gly Leu Phe Gln Asp Thr Ser Ala 
            340                 345                 350 


<210>  5
<211>  24
<212>  DNA
<213>  Escherichia coli

<400>  5
caccatggta gataaacgcg aatc                                              24


<210>  6
<211>  31
<212>  DNA
<213>  Escherichia coli

<400>  6
acattttgtg tagagaaggc gacgtatcct g                                      31


<210>  7
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  7
aagttcaagg cacaatggta gataaacgcg aatc                                   34


<210>  8
<211>  21
<212>  DNA
<213>  Escherichia coli

<400>  8
ttagaaggca gacgtatcct g                                                 21


<210>  9
<211>  30
<212>  DNA
<213>  Escherichia coli

<400>  9
caccatgact actaacactc atactctgca                                        30


<210>  10
<211>  30
<212>  DNA
<213>  Escherichia coli

<400>  10
tacattttgt gtagaggcct cccggctacg                                        30


<210>  11
<211>  41
<212>  DNA
<213>  Escherichia coli

<400>  11
acaagttcaa ggcacaatga ctactaacac tctactctgc a                           41


<210>  12
<211>  19
<212>  DNA
<213>  Escherichia coli

<400>  12
ttaggcctcc cggctacga                                                    19


<210>  13
<211>  22
<212>  DNA
<213>  Escherichia coli

<400>  13
tctacacaaa atgtagcgat tc                                                22


<210>  14
<211>  20
<212>  DNA
<213>  Escherichia coli

<400>  14
tgtgccttga acttgtgctg                                                   20


<210>  15
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Photobacterium Profundum

<400>  15

Ser Thr Gln Asn Val Ala Ile Gln Thr Ala Ala Pro Val Ala Ser Ala 
1               5                   10                  15      


Ser Asn Gly Leu Asp Ala Ala Gln Val Gln Gly Thr 
            20                  25              


<210>  16
<211>  172
<212>  PRT
<213>  Escherichia coli

<400>  16

Met Val Asp Lys Arg Glu Ser Tyr Thr Lys Glu Asp Leu Leu Ala Ser 
1               5                   10                  15      


Gly Arg Gly Glu Leu Phe Gly Ala Lys Gly Pro Gln Leu Pro Ala Pro 
            20                  25                  30          


Asn Met Leu Met Met Asp Arg Val Val Lys Met Thr Glu Thr Gly Gly 
        35                  40                  45              


Asn Phe Asp Lys Gly Tyr Val Glu Ala Glu Leu Asp Ile Asn Pro Asp 
    50                  55                  60                  


Leu Trp Phe Phe Gly Cys His Phe Ile Gly Asp Pro Val Met Pro Gly 
65                  70                  75                  80  


Cys Leu Gly Leu Asp Ala Met Trp Gln Leu Val Gly Phe Tyr Leu Gly 
                85                  90                  95      


Trp Leu Gly Gly Glu Gly Lys Gly Arg Ala Leu Gly Val Gly Glu Val 
            100                 105                 110         


Lys Phe Thr Gly Gln Val Leu Pro Thr Ala Lys Lys Val Thr Tyr Arg 
        115                 120                 125             


Ile His Phe Lys Arg Ile Val Asn Arg Arg Leu Ile Met Gly Leu Ala 
    130                 135                 140                 


Asp Gly Glu Val Leu Val Asp Gly Arg Leu Ile Tyr Thr Ala Ser Asp 
145                 150                 155                 160 


Leu Lys Val Gly Leu Phe Gln Asp Thr Ser Ala Phe 
                165                 170         


<210>  17
<211>  151
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Thr Thr Asn Thr His Thr Leu Gln Ile Glu Glu Ile Leu Glu Leu 
1               5                   10                  15      


Leu Pro His Arg Phe Pro Phe Leu Leu Val Asp Arg Val Leu Asp Phe 
            20                  25                  30          


Glu Glu Gly Arg Phe Leu Arg Ala Val Lys Asn Val Ser Val Asn Glu 
        35                  40                  45              


Pro Phe Phe Gln Gly His Phe Pro Gly Lys Pro Ile Phe Pro Gly Val 
    50                  55                  60                  


Leu Ile Leu Glu Ala Met Ala Gln Ala Thr Gly Ile Leu Ala Phe Lys 
65                  70                  75                  80  


Ser Val Gly Lys Leu Glu Pro Gly Glu Leu Tyr Tyr Phe Ala Gly Ile 
                85                  90                  95      


Asp Glu Ala Arg Phe Lys Arg Pro Val Val Pro Gly Asp Gln Met Ile 
            100                 105                 110         


Met Glu Val Thr Phe Glu Lys Thr Arg Arg Gly Leu Thr Arg Phe Lys 
        115                 120                 125             


Gly Val Ala Leu Val Asp Gly Lys Val Val Cys Glu Ala Thr Met Met 
    130                 135                 140                 


Cys Ala Arg Ser Arg Glu Ala 
145                 150     


