                         SEQUENCE LISTING

<110>  Seattle Children's Hospital (dba Seattle Children's 
       Research Institute)
 
<120>  SODIUM FLUORESCEIN AS A REVERSAL AGENT FOR AN ANTI-FLUORESCEIN 
       CAR T CELLS AND FLUORESCEIN-PHOSOPHOLIPID-ETHERS OR 
       PROFLUORESCEIN-PHOSOPHOLIPID-ETHERS

<130>  SCRI.227WO

<150>  US 62/818,030
<151>  2019-03-13

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(FITC-E2 Mut2) CAR

<400>  1

Ser Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys 
1               5                   10                  15      


Val Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile 
        35                  40                  45              


Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser 
                85                  90                  95      


Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu 
                165                 170                 175     


Ser Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His Phe Ala Ser Tyr Met Asp 
225                 230                 235                 240 


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250     


<210>  2
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(4M5.3) CAR

<400>  2

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Val Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Asn Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Ser Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Asp Ala 
        115                 120                 125             


Lys Lys Asp Asp Ala Lys Lys Asp Gly Gly Val Lys Leu Asp Glu Thr 
    130                 135                 140                 


Gly Gly Gly Leu Val Gln Pro Gly Gly Ala Met Lys Leu Ser Cys Val 
145                 150                 155                 160 


Thr Ser Gly Phe Thr Phe Gly His Tyr Trp Met Asn Trp Val Arg Gln 
                165                 170                 175     


Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Gln Phe Arg Asn Lys Pro 
            180                 185                 190         


Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser Val Lys Gly Arg Phe Thr 
        195                 200                 205             


Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Asn 
    210                 215                 220                 


Leu Arg Val Glu Asp Thr Gly Ile Tyr Tyr Cys Thr Gly Ala Ser Tyr 
225                 230                 235                 240 


Gly Met Glu Tyr Leu Gly Gln Gly Thr Ser Val Thr Val Ser 
                245                 250                 


<210>  3
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(4420) CAR

<400>  3

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Gln Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val 
        115                 120                 125             


Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Arg Pro Met 
    130                 135                 140                 


Lys Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asp Tyr Trp Met 
145                 150                 155                 160 


Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Gln 
                165                 170                 175     


Ile Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr 
        195                 200                 205             


Leu Gln Met Asn Asn Leu Arg Val Glu Asp Met Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  4
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(4D5Flu) CAR

<400>  4

Asp Tyr Lys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Leu 
            20                  25                  30          


Val His Ser Gln Gly Asn Thr Tyr Leu Arg Trp Tyr Gln Gln Lys Pro 
        35                  40                  45              


Gly Lys Ala Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
    50                  55                  60                  


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
65                  70                  75                  80  


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Gln Gln Ser Thr His Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val 
            100                 105                 110         


Glu Leu Lys Arg Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
145                 150                 155                 160 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                165                 170                 175     


Ser Asp Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
            180                 185                 190         


Glu Trp Val Ala Gln Ile Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr 
        195                 200                 205             


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Thr Ser 
    210                 215                 220                 


Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
225                 230                 235                 240 


Ala Val Tyr Tyr Cys Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp Gly 
                245                 250                 255     


Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265 


<210>  5
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(FITCE2) CAR

<400>  5

Ser Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys 
1               5                   10                  15      


Val Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile 
        35                  40                  45              


Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser 
                85                  90                  95      


Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu 
                165                 170                 175     


Ser Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His Phe Tyr Ser Tyr Met Asp 
225                 230                 235                 240 


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250     


<210>  6
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antiFL(FITCE2 HisH131Ala) CAR

<400>  6

Ser Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys 
1               5                   10                  15      


Val Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile 
        35                  40                  45              


Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser 
                85                  90                  95      


Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu 
                165                 170                 175     


Ser Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


Ser Tyr Asp Ser Ser Gly Tyr Trp Gly Ala Phe Tyr Ser Tyr Met Asp 
225                 230                 235                 240 


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250     


