                         SEQUENCE LISTING RECTIFIED UNDER RULE 91 ISA/EP

<110>  President and Fellows of Harvard College
       The Broad Institute, Inc.
       The General Hospital Corporation
       Sabeti, Pardis
       Rosenberg, Eric
       Adams, Gordon
       Lemieux, Jacob
       Piantadosi, Anne
       Normandin, Erica
 
<120>  Sherlock Assays for Tick-Borne Diseases

<130>  BROD-4100WP

<140>  PCT/US20/22776
<141>  2020-03-13

<150>  US 62/818,739
<151>  2019-03-14

<150>  US 62/860,225
<151>  2019-06-11

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  55
<212>  PRT
<213>  Babesia microti

<400>  1

Met Tyr Ala His Leu Ile Lys Gly Leu Trp Tyr Ser Cys Thr Tyr Leu 
1               5                   10                  15      


Pro Leu Ser Trp Tyr Thr Gly Ile Val Ile Leu Met Leu Ser Tyr Ala 
            20                  25                  30          


Ile Gly Phe Met Gly Tyr Val Leu Pro Tyr Gly Gln Ser Tyr Trp Gly 
        35                  40                  45              


Ala Thr Val Ile Ile Asn Leu 
    50                  55  


<210>  2
<211>  26
<212>  PRT
<213>  Babesia microti

<400>  2

Leu His Ile Val Pro Glu Trp Tyr Leu Leu Pro Phe Tyr Gly Thr Leu 
1               5                   10                  15      


Lys Leu Leu Pro Thr Lys Leu Ser Gly Leu 
            20                  25      


<210>  3
<211>  36
<212>  PRT
<213>  Babesia microti

<400>  3

Ser Lys Phe Asn Ser Ser Phe Ser Ser Lys Lys Ile Arg Ser Gln Lys 
1               5                   10                  15      


Ser Ser Gly Lys Ser Arg Ile Lys Thr Lys Ser Thr Asn Ile Phe Val 
            20                  25                  30          


Gly Gly Tyr Tyr 
        35      


<210>  4
<211>  48
<212>  PRT
<213>  Borrelia bavariensis

<400>  4

Met Tyr Val Ser Leu Ile Ala Asn Thr Ile Asp Phe Asp Lys Ile Asn 
1               5                   10                  15      


Tyr Ile Asn Ser Lys Met Leu Leu Asp Leu Ile Arg Asn Val Glu Leu 
            20                  25                  30          


Leu Val Leu Glu Phe Ala Lys Tyr Ser Asp Phe Lys Leu Gly Lys Ile 
        35                  40                  45              


<210>  5
<211>  87
<212>  PRT
<213>  Brucella abortis


<220>
<221>  MISC_FEATURE
<222>  (1)..(87)
<223>  X may represent any amino acid or may indicate a residue not 
       present

<400>  5

Ser Pro Asp Met Ala Ile Leu Asn Leu Ser Val Leu Arg Gln Ala Lys 
1               5                   10                  15      


Thr Ala Arg Glu Ala Met Thr Ala Asn Asn Glu Ala Met Thr Lys Val 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Asp Ala Met Lys Lys Ala Gly 
        35                  40                  45              


Ile Xaa Xaa Xaa Xaa Xaa Xaa Glu Asp Arg Asp Leu Gln Thr Gly Gly 
    50                  55                  60                  


Ile Asp Ile Gln Pro Ile Tyr Val Tyr Pro Asp Asp Lys Asn Asn Leu 
65                  70                  75                  80  


Lys Glu Pro Thr Ile Thr Gly 
                85          


<210>  6
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents Babesia mictori cytB Consensus Sequence

<400>  6
ctrcgctatt ggcttcatgg gatatgtatt accatatggc caaatkagct actggggagc       60

tam                                                                     63


<210>  7
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents R1-2005

<400>  7
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

tac                                                                     63


<210>  8
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents Gray-1971

<400>  8
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

tac                                                                     63


<210>  9
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents Bab05-2014

<400>  9
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

tac                                                                     63


<210>  10
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents Bab14-2015

<400>  10
ctgcgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

tac                                                                     63


<210>  11
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents MGH-2001

<400>  11
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaattagct actggggagc       60

tac                                                                     63


<210>  12
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents BWH-2003

<400>  12
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

tac                                                                     63


<210>  13
<211>  63
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(63)
<223>  Sequence represents MORNS-2015

<400>  13
ctacgctatt ggcttcatgg gatatgtatt accatatggc caaatgagct actggggagc       60

taa                                                                     63


<210>  14

<400>  14
000

<210>  15
<211>  70
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(70)
<223>  Sequence represents Babesia mictori rpI4 Consensus Sequence

<400>  15
aagtaaattt aatagttctt ttagttyaaa aaaaattaga tctcaaaaat cttcaggaaa       60

atcayrtata                                                              70


<210>  16
<211>  29
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  Sequence represents Bab.rpI4.R86C

<400>  16
atctcaaaaa tcttcaggaa aatcatgta                                         29


<210>  17
<211>  70
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(70)
<223>  Sequence represents rpI4_RI

<400>  17
aagtaaattt aatagttctt ttagttcaaa aaaaattaga tctcaaaaat cttcaggaaa       60

atcacgtata                                                              70


<210>  18
<211>  70
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(70)
<223>  Sequence represents rpI4_Bab05

<400>  18
aagtaaattt aatagttctt ttagttcaaa aaaaattaga tctcaaaaat cttcaggaaa       60

atcacatata                                                              70


<210>  19
<211>  70
<212>  DNA
<213>  Babesia mictori


<220>
<221>  misc_feature
<222>  (1)..(70)
<223>  Sequence represents rpI4_MORNS2...

<400>  19
aagtaaattt aatagttctt ttagtttaaa aaaaattaga tctcaaaaat cttcaggaaa       60

atcacatata                                                              70


<210>  20
<211>  46
<212>  DNA
<213>  Anaplasma phagocytphilum


<220>
<221>  misc_feature
<222>  (1)..(46)
<223>  Sequence represents16S guide Consensus Sequence

<400>  20
cactagaaat ggtgggtaat actgtataat ccctgcgggg gaaaga                      46


<210>  21
<211>  33
<212>  DNA
<213>  Anaplasma phagocytphillum


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  Sequence represents Anaplasma phagocytphilum metallloprotease 
       guide Consensus Sequence

<400>  21
tccttctgcc gtatcgcatc accagwaygc ttt                                    33


<210>  22
<211>  33
<212>  DNA
<213>  Anaplasma phagocytphilum


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  Sequence represents Equi gBlock

<400>  22
tccttctgcc gtatcgcatc accagaacgc ttt                                    33


<210>  23
<211>  28
<212>  DNA
<213>  Anaplasma phagocytphilum


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  Sequence represents Equi Guide

<400>  23
cttctgccgt atcgcatcac cagtacgc                                          28


<210>  24
<211>  33
<212>  DNA
<213>  Anaplasma phagosytphilum


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  Sequence represents WT gBlock

<400>  24
tccttctgcc gtatcgcatc accagaatgc ttt                                    33


<210>  25
<211>  28
<212>  DNA
<213>  Anaplasma phagocytphilum


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  Sequence represents WT Guide

<400>  25
cttctgccgt atcgcatcac cagtatgc                                          28


<210>  26
<211>  48
<212>  DNA
<213>  Borrelia miyamotoi


<220>
<221>  misc_feature
<222>  (1)..(48)
<223>  Sequence represents Borrelia miyamotoi gIpQ guide

<400>  26
ccacaaatgt tgcacaatta tttcccaatc gagctagaga aaacggam                    48


<210>  27
<211>  48
<212>  DNA
<213>  Borrelia miyamotoi


<220>
<221>  misc_feature
<222>  (1)..(48)
<223>  Sequence represents Borrelia miyamotoi gIpQ guide

<400>  27
ccacaaatgt tgcacaatta tttcccaatc gagctagaga aaacggam                    48


<210>  28
<211>  48
<212>  DNA
<213>  Borrelia miyamotoi


<220>
<221>  misc_feature
<222>  (1)..(48)
<223>  Sequence represents Borrelia miyamotoi gIpQ guide

<400>  28
ccacaaatgt tgcacaatta tttcccaatc gagctagaga aaacggac                    48


<210>  29
<211>  51
<212>  DNA
<213>  Borrelia miyamotoi


<220>
<221>  misc_feature
<222>  (1)..(51)
<223>  Sequence represents Borrelia miyamotoi flab guide

<400>  29
tctgatgatg ctgctggtat gggtgttgct ggtaagctta attcacaaat t                51


<210>  30
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30
ucuagaaaua uggauuacuu gguagaacag caaucuacuc gaccugcagg caugcaa          57


<210>  31
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31
gauuuagacu accccaaaaa cgaaggggac uaaaacuaga uugcuguucu accaaguaau       60

ccau                                                                    64


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32
gggaacaaag cugaaguacu uaccc                                             25


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33
gggtagggcg ggttggga                                                     18


<210>  34
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Biotin tag /5Biosyg/

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Iowa black quencher /3IAbRQSp/

<400>  34
ucucguacgu uc                                                           12


<210>  35
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sythetic


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Biotin Tag /5Biosg/

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Iowa black quencher /3IAbRQSp/

<400>  35
ucucguacgu ucucucguac guuc                                              24


