SEQUENCE LISTING

<110>   CSL Behring Lengnau AG

<120>   Factor IX variants and uses thereof in therapy

<130>   2019_L001_A301

<140>   EP19163619.0
<141>   2019-03-19

<160>   23

<170>   SeqWin2010, version 1.0

<210>   1
<211>   415
<212>   PRT
<213>   Homo sapiens

<400>   1
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   2
<211>   1251
<212>   DNA
<213>   Homo sapiens

<400>   2
atgtataatt caggtaaatt ggaagagttt gttcaaggga accttgagag agaatgtatg  60
gaagaaaagt gtagttttga agaagcacga gaagtttttg aaaacactga aagaacaact  120
gaattttgga agcagtatgt tgatggagat cagtgtgagt ccaatccatg tttaaatggc  180
ggcagttgca aggatgacat taattcctat gaatgttggt gtccctttgg atttgaagga  240
aagaactgtg aattagatgt aacatgtaac attaagaatg gcagatgcga gcagttttgt  300
aaaaatagtg ctgataacaa ggtggtttgc tcctgtactg agggatatcg acttgcagaa  360
aaccagaagt cctgtgaacc agcagtgcca tttccatgtg gaagagtttc tgtttcacaa  420
acttctaagc tcacccgtgc tgagactgtt tttcctgatg tggactatgt aaattctact  480
gaagctgaaa ccattttgga taacatcact caaagcaccc aatcatttaa tgacttcact  540
cgggttgttg gtggagaaga tgccaaacca ggtcaattcc cttggcaggt tgttttgaat  600
ggtaaagttg atgcattctg tggaggctct atcgttaatg aaaaatggat tgtaactgct  660
gcccactgtg ttgaaactgg tgttaaaatt acagttgtcg caggtgaaca taatattgag  720
gagacagaac atacagagca aaagcgaaat gtgattcgaa ttattcctca ccacaactac  780
aatgcagcta ttaataagta caaccatgac attgcccttc tggaactgga cgaaccctta  840
gtgctaaaca gctacgttac acctatttgc attgctgaca aggaatacac gaacatcttc  900
ctcaaatttg gatctggcta tgtaagtggc tggggaagag tcttccacaa agggagatca  960
gctttagttc ttcagtacct tagagttcca cttgttgacc gagccacatg tcttcgatct  1020
acaaagttca ccatctataa caacatgttc tgtgctggct tccatgaagg aggtagagat  1080
tcatgtcaag gagatagtgg gggaccccat gttactgaag tggaagggac cagtttctta  1140
actggaatta ttagctgggg tgaagagtgt gcaatgaaag gcaaatatgg aatatatacc  1200
aaggtatccc ggtatgtcaa ctggattaag gaaaaaacaa agctcactta a           1251

<210>   3
<211>   461
<212>   PRT
<213>   Homo sapiens

<400>   3
Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      

Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          

Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              

Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  

Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  

Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      

Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         

Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             

Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 

Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 

Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     

Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         

Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             

Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 

Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 

Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     

Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         

Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             

His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 

Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 

Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     

Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         

Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             

Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg 
    370                 375                 380                 

Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 

Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     

Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         

Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             

Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
    450                 455                 460     

<210>   4
<211>   1386
<212>   DNA
<213>   Homo sapiens

<400>   4
atgcagcgcg tgaacatgat catggcagaa tcaccaggcc tcatcaccat ctgcctttta  60
ggatatctac tcagtgctga atgtacagtt tttcttgatc atgaaaacgc caacaaaatt  120
ctgaatcggc caaagaggta taattcaggt aaattggaag agtttgttca agggaacctt  180
gagagagaat gtatggaaga aaagtgtagt tttgaagaag cacgagaagt ttttgaaaac  240
actgaaagaa caactgaatt ttggaagcag tatgttgatg gagatcagtg tgagtccaat  300
ccatgtttaa atggcggcag ttgcaaggat gacattaatt cctatgaatg ttggtgtccc  360
tttggatttg aaggaaagaa ctgtgaatta gatgtaacat gtaacattaa gaatggcaga  420
tgcgagcagt tttgtaaaaa tagtgctgat aacaaggtgg tttgctcctg tactgaggga  480
tatcgacttg cagaaaacca gaagtcctgt gaaccagcag tgccatttcc atgtggaaga  540
gtttctgttt cacaaacttc taagctcacc cgtgctgaga ctgtttttcc tgatgtggac  600
tatgtaaatt ctactgaagc tgaaaccatt ttggataaca tcactcaaag cacccaatca  660
tttaatgact tcactcgggt tgttggtgga gaagatgcca aaccaggtca attcccttgg  720
caggttgttt tgaatggtaa agttgatgca ttctgtggag gctctatcgt taatgaaaaa  780
tggattgtaa ctgctgccca ctgtgttgaa actggtgtta aaattacagt tgtcgcaggt  840
gaacataata ttgaggagac agaacataca gagcaaaagc gaaatgtgat tcgaattatt  900
cctcaccaca actacaatgc agctattaat aagtacaacc atgacattgc ccttctggaa  960
ctggacgaac ccttagtgct aaacagctac gttacaccta tttgcattgc tgacaaggaa  1020
tacacgaaca tcttcctcaa atttggatct ggctatgtaa gtggctgggg aagagtcttc  1080
cacaaaggga gatcagcttt agttcttcag taccttagag ttccacttgt tgaccgagcc  1140
acatgtcttc gatctacaaa gttcaccatc tataacaaca tgttctgtgc tggcttccat  1200
gaaggaggta gagattcatg tcaaggagat agtgggggac cccatgttac tgaagtggaa  1260
gggaccagtt tcttaactgg aattattagc tggggtgaag agtgtgcaat gaaaggcaaa  1320
tatggaatat ataccaaggt atcccggtat gtcaactgga ttaaggaaaa aacaaagctc  1380
acttaa                                                             1386

<210>   5
<211>   145
<212>   PRT
<213>   Homo sapiens

<400>   5
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg 
145 

<210>   6
<211>   235
<212>   PRT
<213>   Homo sapiens

<400>   6
Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp Gln Val 
1               5                   10                  15      

Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile Val Asn 
            20                  25                  30          

Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly Val Lys 
        35                  40                  45              

Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu His Thr 
    50                  55                  60                  

Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn Tyr Asn 
65                  70                  75                  80  

Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu Leu Asp 
                85                  90                  95      

Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile Ala Asp 
            100                 105                 110         

Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr Val Ser 
        115                 120                 125             

Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val Leu Gln 
    130                 135                 140                 

Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg Ser Thr 
145                 150                 155                 160 

Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His Glu Gly 
                165                 170                 175     

Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val Thr Glu 
            180                 185                 190         

Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly Glu Glu 
        195                 200                 205             

Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser Arg Tyr 
    210                 215                 220                 

Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
225                 230                 235 

<210>   7
<211>   415
<212>   PRT
<213>   Homo sapiens

<400>   7
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Ala Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   8
<211>   18
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   8
Pro Val Ser Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro 
1               5                   10                  15      

Asp Val 
        

<210>   9
<211>   585
<212>   PRT
<213>   Homo sapiens

<400>   9
Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      

Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          

Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              

Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  

Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  

Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      

Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         

Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             

Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 

Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 

Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     

Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         

Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             

Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 

Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 

Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     

Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         

Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             

Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 

Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 

Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     

Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         

Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             

Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 

Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 

Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     

Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         

Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             

Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 

Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 

Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     

Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         

Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             

Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 

Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 

Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     

Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 

<210>   10
<211>   1018
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX (wild-type) albumin fusion protein

<400>   10
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr Pro 
                405                 410                 415     

Val Ser Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp 
            420                 425                 430         

Val Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly 
        435                 440                 445             

Glu Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu 
    450                 455                 460                 

Gln Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr 
465                 470                 475                 480 

Glu Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp 
                485                 490                 495     

Lys Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr 
            500                 505                 510         

Leu Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu 
        515                 520                 525             

Pro Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn 
    530                 535                 540                 

Leu Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe 
545                 550                 555                 560 

His Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala 
                565                 570                 575     

Arg Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys 
            580                 585                 590         

Arg Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala 
        595                 600                 605             

Ala Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala 
    610                 615                 620                 

Ser Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly 
625                 630                 635                 640 

Glu Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe 
                645                 650                 655     

Pro Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr 
            660                 665                 670         

Lys Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp 
        675                 680                 685             

Asp Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile 
    690                 695                 700                 

Ser Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser 
705                 710                 715                 720 

His Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro 
                725                 730                 735     

Ser Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr 
            740                 745                 750         

Ala Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala 
        755                 760                 765             

Arg Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys 
    770                 775                 780                 

Thr Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His 
785                 790                 795                 800 

Glu Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu 
                805                 810                 815     

Pro Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly 
            820                 825                 830         

Glu Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val 
        835                 840                 845             

Pro Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly 
    850                 855                 860                 

Lys Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro 
865                 870                 875                 880 

Cys Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu 
                885                 890                 895     

His Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu 
            900                 905                 910         

Ser Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu 
        915                 920                 925             

Thr Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala 
    930                 935                 940                 

Asp Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr 
945                 950                 955                 960 

Ala Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln 
                965                 970                 975     

Leu Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys 
            980                 985                 990         

Lys Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu 
        995                 1000                1005            

Val Ala Ala Ser Gln Ala Ala Leu Gly Leu 
    1010                1015            

<210>   11
<211>   415
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX variant R338V/E410H

<400>   11
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Val Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys His Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   12
<211>   415
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX variant R338T/E410H

<400>   12
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Thr Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys His Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   13
<211>   415
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX variant R338W/E410H

<400>   13
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Trp Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys His Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   14
<211>   415
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX variant R338L/E410H

<400>   14
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Leu Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys His Lys Thr Lys Leu Thr 
                405                 410                 415 

<210>   15
<211>   1018
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   FIX variant R338V/E410H albumin fusion

<400>   15
Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      

Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          

Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              

Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  

Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  

Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      

Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         

Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             

Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 

Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 

Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     

Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         

Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             

Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 

Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 

Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     

His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         

Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             

Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 

Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 

Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     

Leu Val Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         

Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             

His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 

Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 

Val Ser Arg Tyr Val Asn Trp Ile Lys His Lys Thr Lys Leu Thr Pro 
                405                 410                 415     

Val Ser Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp 
            420                 425                 430         

Val Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly 
        435                 440                 445             

Glu Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu 
    450                 455                 460                 

Gln Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr 
465                 470                 475                 480 

Glu Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp 
                485                 490                 495     

Lys Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr 
            500                 505                 510         

Leu Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu 
        515                 520                 525             

Pro Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn 
    530                 535                 540                 

Leu Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe 
545                 550                 555                 560 

His Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala 
                565                 570                 575     

Arg Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys 
            580                 585                 590         

Arg Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala 
        595                 600                 605             

Ala Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala 
    610                 615                 620                 

Ser Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly 
625                 630                 635                 640 

Glu Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe 
                645                 650                 655     

Pro Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr 
            660                 665                 670         

Lys Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp 
        675                 680                 685             

Asp Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile 
    690                 695                 700                 

Ser Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser 
705                 710                 715                 720 

His Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro 
                725                 730                 735     

Ser Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr 
            740                 745                 750         

Ala Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala 
        755                 760                 765             

Arg Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys 
    770                 775                 780                 

Thr Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His 
785                 790                 795                 800 

Glu Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu 
                805                 810                 815     

Pro Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly 
            820                 825                 830         

Glu Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val 
        835                 840                 845             

Pro Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly 
    850                 855                 860                 

Lys Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro 
865                 870                 875                 880 

Cys Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu 
                885                 890                 895     

His Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu 
            900                 905                 910         

Ser Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu 
        915                 920                 925             

Thr Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala 
    930                 935                 940                 

Asp Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr 
945                 950                 955                 960 

Ala Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln 
                965                 970                 975     

Leu Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys 
            980                 985                 990         

Lys Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu 
        995                 1000                1005            

Val Ala Ala Ser Gln Ala Ala Leu Gly Leu 
    1010                1015            

<210>   16
<211>   231
<212>   PRT
<213>   Homo sapiens

<400>   16
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      

Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          

Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              

Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  

Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  

Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      

Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         

Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             

Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 

Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 

Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     

Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         

Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             

Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 

Ser Leu Ser Leu Ser Pro Gly 
225                 230     

<210>   17
<211>   226
<212>   PRT
<213>   Homo sapiens

<400>   17
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 

Pro Gly 
225     

<210>   18
<211>   10
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CTP sequence

<400>   18
Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser 
1               5                   10  

<210>   19
<211>   32
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CTP sequence

<400>   19
Asp Pro Arg Phe Gln Asp Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser 
1               5                   10                  15      

Leu Pro Ser Pro Ser Arg Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu 
            20                  25                  30          

<210>   20
<211>   28
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   CTP sequence

<400>   20
Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      

Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln 
            20                  25              

<210>   21
<211>   78
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   XTEN artificial sequence

<400>   21
Gly Ala Pro Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser 
1               5                   10                  15      

Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala 
            20                  25                  30          

Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser Gly Ser Glu 
        35                  40                  45              

Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Thr 
    50                  55                  60                  

Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Ala Ser Ser 
65                  70                  75              

<210>   22
<211>   42
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   XTEN artificial sequence

<400>   22
Gly Ala Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly 
1               5                   10                  15      

Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala 
            20                  25                  30          

Thr Ser Gly Ser Glu Thr Pro Ala Ser Ser 
        35                  40          

<210>   23
<211>   7
<212>   PRT
<213>   Artificial Sequence

<220>
<223>   Linker

<400>   23
Gly Gly Gly Gly Gly Gly Val 
1               5           

