                         SEQUENCE LISTING

<110>  Cambridge enterprise limited
 
<120>  Methods of altering mitochondrial heteroplasmy

<130>  PC928814GB

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mitochondrial localisation signal 1

<400>  1

Met Leu Gly Phe Val Gly Arg Val Ala Ala Ala Pro Ala Ser Gly Ala 
1               5                   10                  15      


Leu Arg Arg Leu Thr Pro Ser Ala Ser Leu Pro Pro Ala Gln Leu Leu 
            20                  25                  30          


Leu Arg Ala Ala Pro Thr Ala Val His Pro Val Arg Asp Tyr Ala Ala 
        35                  40                  45              


Gln 
    


<210>  2
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mitochondrial localisation signal 2

<400>  2

Pro Met Leu Gly Phe Val Gly Arg Val Ala Ala Ala Pro Ala Ser Gly 
1               5                   10                  15      


Ala Leu Arg Arg Leu Thr Pro Ser Ala Ser Leu Pro Pro Ala Gln Leu 
            20                  25                  30          


Leu Leu Arg Ala Ala Pro Thr Ala Val His Pro Val Arg Asp Tyr Ala 
        35                  40                  45              


Ala Gln 
    50  


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA epitope tag

<400>  3

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  4
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nuclear export tag

<400>  4

Val Asp Glu Met Thr Lys Lys Phe Gly Thr Leu Thr Ile His Asp Thr 
1               5                   10                  15      


Glu Lys 
        


<210>  5
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zn Finger protein 1

<400>  5

Met Ala Glu Arg Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser 
1               5                   10                  15      


Gly Asn Thr Gly Leu Asn Cys His Ile Arg Thr His Thr Gly Glu Lys 
            20                  25                  30          


Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe Ala Asp Arg Ser Asn 
        35                  40                  45              


Leu Thr Arg His Thr Lys Ile His Thr His Pro Arg Ala Pro Ile Pro 
    50                  55                  60                  


Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser Gln Ser Gly 
65                  70                  75                  80  


Ser Leu Thr Arg His Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala 
                85                  90                  95      


Cys Asp Ile Cys Gly Arg Lys Phe Ala His Lys Ser Ala Arg Ala Ala 
            100                 105                 110         


His Thr Lys Ile His Thr Gly Ser Gln Lys Pro Phe Gln Cys Arg Ile 
        115                 120                 125             


Cys Met Arg Asn Phe Ser Arg Ser Asp His Leu Ser Ala His Ile Arg 
    130                 135                 140                 


Thr His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys 
145                 150                 155                 160 


Phe Ala Gln His Gly Ser Leu Ala Ser His Thr Lys Ile His Leu Arg 
                165                 170                 175     


<210>  6
<211>  196
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FokI catalytic domain 1

<400>  6

Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
1               5                   10                  15      


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
            20                  25                  30          


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
        35                  40                  45              


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
65                  70                  75                  80  


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
                85                  90                  95      


Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Lys Glu Asn Gln Thr Arg 
            100                 105                 110         


Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
        115                 120                 125             


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
    130                 135                 140                 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn Arg Lys Thr Asn Cys Asn Gly 
145                 150                 155                 160 


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
                165                 170                 175     


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
            180                 185                 190         


Glu Ile Asn Phe 
        195     


<210>  7
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zn Finger protein 2

<400>  7

Met Ala Glu Arg Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser 
1               5                   10                  15      


Leu Pro His His Leu Glu Gln His Ile Arg Thr His Thr Gly Glu Lys 
            20                  25                  30          


Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe Ala Arg Asn Ala Ser 
        35                  40                  45              


Arg Thr Arg His Thr Lys Ile His Thr Gly Ser Gln Lys Pro Phe Gln 
    50                  55                  60                  


Cys Arg Ile Cys Met Arg Lys Phe Ala Tyr Thr Tyr Ser Leu Ser Glu 
65                  70                  75                  80  


His Thr Lys Ile His Thr Gly Glu Lys Pro Phe Gln Cys Arg Ile Cys 
                85                  90                  95      


Met Arg Asn Phe Ser Gln Ser Ala Asn Arg Thr Thr His Ile Arg Thr 
            100                 105                 110         


His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe 
        115                 120                 125             


Ala His Arg Ser Ser Leu Arg Arg His Thr Lys Ile His Leu Arg 
    130                 135                 140             


<210>  8
<211>  196
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FokI catalytic domain 2

<400>  8

Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
1               5                   10                  15      


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
            20                  25                  30          


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
        35                  40                  45              


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
65                  70                  75                  80  


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
                85                  90                  95      


Gly Gln Ala Asp Glu Met Glu Arg Tyr Val Glu Glu Asn Gln Thr Arg 
            100                 105                 110         


Asp Lys His Leu Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
        115                 120                 125             


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
    130                 135                 140                 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly 
145                 150                 155                 160 


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
                165                 170                 175     


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
            180                 185                 190         


Glu Ile Asn Phe 
        195     


<210>  9
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A sequence

<400>  9

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly 
    


<210>  10
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mitochondrial localisation signal 1

<400>  10
atgttggggt ttgtgggtcg ggtggccgct gctccggcct ccggggcctt gcggagactc       60

accccttcag cgtcgctgcc cccagctcag ctcttactgc gggccgctcc gacggcggtc      120

catcctgtca gggactatgc ggcgcaa                                          147


<210>  11
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mitochondrial localisation signal 2

<400>  11
atgctcggtt tcgtaggccg cgtcgctgcc gcaccagctt caggtgcact ccgccgattg       60

acacccagcg caagccttcc tcccgcacag ttgttgctcc gagctgcccc caccgccgtt      120

caccccgtgc gagattacgc agctcag                                          147


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HA epitope tag

<400>  12
tacccctacg acgtgcccga ctacgcc                                           27


<210>  13
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nuclear export tag

<400>  13
gtggatgaaa tgaccaaaaa gttcggcacg ctcaccattc acgacaccga aaag             54


<210>  14
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zn Finger protein 1

<400>  14
atggctgaga ggcccttcca gtgtcgaatc tgcatgcgta acttcagtgg caacaccggc       60

ctgaactgtc acatccgcac ccacaccggc gagaagcctt ttgcctgtga catttgtggg      120

aggaaatttg ccgaccgctc caacctgacc cgccatacca agatacacac gcatcccagg      180

gcacctattc ccaagccctt ccagtgtcga atctgcatgc gtaacttcag tcagtccggc      240

tccctgaccc gccacatccg cacccacacc ggcgagaagc cttttgcctg tgacatttgt      300

gggaggaaat ttgcccacaa gtccgcccgc gccgcccata ccaagataca cacgggatct      360

cagaagccct tccagtgtcg aatctgcatg cgtaacttca gtcgctccga ccacctgtcc      420

gcccacatcc gcacccacac cggcgagaag ccttttgcct gtgacatttg tgggaggaaa      480

tttgcccagc acggctccct ggcctcccat accaagatac acctgcgg                   528


<210>  15
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FokI catalytic domain 1

<400>  15
cagctggtga agagcgagct ggaggagaag aagtccgagc tgcggcacaa gctgaagtac       60

gtgccccacg agtacatcga gctgatcgag atcgccagga acagcaccca ggaccgcatc      120

ctggagatga aggtgatgga gttcttcatg aaggtgtacg gctacagggg aaagcacctg      180

ggcggaagca gaaagcctga cggcgccatc tatacagtgg gcagccccat cgattacggc      240

gtgatcgtgg acacaaaggc ctacagcggc ggctacaatc tgcctatcgg ccaggccgac      300

gagatgcaga gatacgtgaa ggagaaccag acccggaata agcacatcaa ccccaacgag      360

tggtggaagg tgtaccctag cagcgtgacc gagttcaagt tcctgttcgt gagcggccac      420

ttcaagggca actacaaggc ccagctgacc aggctgaacc gcaagaccaa ctgcaatggc      480

gccgtgctga gcgtggagga gctgctgatc ggcggcgaga tgatcaaagc cggcaccctg      540

acactggagg aggtgcggcg caagttcaac aacggcgaga tcaacttc                   588


<210>  16
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zn Finger protein 2

<400>  16
atggccgaac gcccttttca atgccggatt tgtatgagaa atttttctct tcctcaccac       60

ctggagcaac atattaggac acatactggg gaaaaaccct tcgcttgcga tatctgcgga      120

cgcaagttcg ctcggaatgc ttcccgcact cgacacacaa aaatccatac agggtcccag      180

aagccattcc aatgcaggat ctgcatgaga aagttcgcat acacctactc tctctctgag      240

catactaaaa ttcacactgg ggaaaaacca tttcaatgca gaatatgtat gcgaaatttc      300

tcccagagtg ctaatcggac cactcatatt cgaacacata caggagaaaa acccttcgct      360

tgcgatatct gcggacgaaa gttcgctcat aggagtagcc tccgccgcca cacaaaaatc      420

catcttcgc                                                              429


<210>  17
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FokI catalytic domain 2

<400>  17
caacttgtta aatcagaact cgaagaaaaa aaaagcgagc tacgccataa actcaaatat       60

gtacctcatg aatatattga attaattgaa attgcaagaa atagtacaca agatcgaatt      120

ttggaaatga aagtcatgga attttttatg aaagtatatg gttaccgcgg caaacatctt      180

ggaggatcaa ggaaaccaga tggggcaatt tacactgttg ggagtcctat agactacggg      240

gtcattgtcg ataccaaagc ttattctgga gggtataacc ttcccattgg tcaagctgat      300

gaaatggagc gctatgtaga agaaaatcaa acaagagaca aacatcttaa ccctaatgaa      360

tggtggaaag tctatcccag ttctgttact gaatttaaat ttctctttgt ctctggacat      420

tttaaaggaa attataaagc tcaactcaca agattaaatc atataacaaa ttgtaacggt      480

gctgtactct cagtcgaaga actcctcatt ggaggtgaaa tgataaaggc tggaacactc      540

accctcgaag aagttcgccg aaaatttaat aatggggaaa ttaatttt                   588


<210>  18
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T2A sequence

<400>  18
gaagggagag gatctctgct tacttgcggc gatgtagagg aaaaccccgg accc             54


<210>  19
<211>  895
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Complete MTM25(+)_T2A_WTM1(-) amino acid sequence

<400>  19

Met Leu Gly Phe Val Gly Arg Val Ala Ala Ala Pro Ala Ser Gly Ala 
1               5                   10                  15      


Leu Arg Arg Leu Thr Pro Ser Ala Ser Leu Pro Pro Ala Gln Leu Leu 
            20                  25                  30          


Leu Arg Ala Ala Pro Thr Ala Val His Pro Val Arg Asp Tyr Ala Ala 
        35                  40                  45              


Gln Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Val Asp Glu Met Thr Lys 
    50                  55                  60                  


Lys Phe Gly Thr Leu Thr Ile His Asp Thr Glu Lys Ala Ala Glu Phe 
65                  70                  75                  80  


Met Ala Glu Arg Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser 
                85                  90                  95      


Gly Asn Thr Gly Leu Asn Cys His Ile Arg Thr His Thr Gly Glu Lys 
            100                 105                 110         


Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe Ala Asp Arg Ser Asn 
        115                 120                 125             


Leu Thr Arg His Thr Lys Ile His Thr His Pro Arg Ala Pro Ile Pro 
    130                 135                 140                 


Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser Gln Ser Gly 
145                 150                 155                 160 


Ser Leu Thr Arg His Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala 
                165                 170                 175     


Cys Asp Ile Cys Gly Arg Lys Phe Ala His Lys Ser Ala Arg Ala Ala 
            180                 185                 190         


His Thr Lys Ile His Thr Gly Ser Gln Lys Pro Phe Gln Cys Arg Ile 
        195                 200                 205             


Cys Met Arg Asn Phe Ser Arg Ser Asp His Leu Ser Ala His Ile Arg 
    210                 215                 220                 


Thr His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys 
225                 230                 235                 240 


Phe Ala Gln His Gly Ser Leu Ala Ser His Thr Lys Ile His Leu Arg 
                245                 250                 255     


Gly Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu 
            260                 265                 270         


Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu 
        275                 280                 285             


Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met 
    290                 295                 300                 


Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly 
305                 310                 315                 320 


Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp 
                325                 330                 335     


Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu 
            340                 345                 350         


Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Lys Glu Asn Gln 
        355                 360                 365             


Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro 
    370                 375                 380                 


Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys 
385                 390                 395                 400 


Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn Arg Lys Thr Asn Cys 
                405                 410                 415     


Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met 
            420                 425                 430         


Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn 
        435                 440                 445             


Asn Gly Glu Ile Asn Phe Leu Glu Glu Gly Arg Gly Ser Leu Leu Thr 
    450                 455                 460                 


Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu Gly Phe Val Gly 
465                 470                 475                 480 


Arg Val Ala Ala Ala Pro Ala Ser Gly Ala Leu Arg Arg Leu Thr Pro 
                485                 490                 495     


Ser Ala Ser Leu Pro Pro Ala Gln Leu Leu Leu Arg Ala Ala Pro Thr 
            500                 505                 510         


Ala Val His Pro Val Arg Asp Tyr Ala Ala Gln Tyr Pro Tyr Asp Val 
        515                 520                 525             


Pro Asp Tyr Ala Val Asp Glu Met Thr Lys Lys Phe Gly Thr Leu Thr 
    530                 535                 540                 


Ile His Asp Thr Glu Lys Ala Ala Glu Phe Met Ala Glu Arg Pro Phe 
545                 550                 555                 560 


Gln Cys Arg Ile Cys Met Arg Asn Phe Ser Leu Pro His His Leu Glu 
                565                 570                 575     


Gln His Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile 
            580                 585                 590         


Cys Gly Arg Lys Phe Ala Arg Asn Ala Ser Arg Thr Arg His Thr Lys 
        595                 600                 605             


Ile His Thr Gly Ser Gln Lys Pro Phe Gln Cys Arg Ile Cys Met Arg 
    610                 615                 620                 


Lys Phe Ala Tyr Thr Tyr Ser Leu Ser Glu His Thr Lys Ile His Thr 
625                 630                 635                 640 


Gly Glu Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser Gln 
                645                 650                 655     


Ser Ala Asn Arg Thr Thr His Ile Arg Thr His Thr Gly Glu Lys Pro 
            660                 665                 670         


Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe Ala His Arg Ser Ser Leu 
        675                 680                 685             


Arg Arg His Thr Lys Ile His Leu Arg Gly Ser Gln Leu Val Lys Ser 
    690                 695                 700                 


Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val 
705                 710                 715                 720 


Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln 
                725                 730                 735     


Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe Phe Met Lys Val Tyr 
            740                 745                 750         


Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala 
        755                 760                 765             


Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr 
    770                 775                 780                 


Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu 
785                 790                 795                 800 


Met Glu Arg Tyr Val Glu Glu Asn Gln Thr Arg Asp Lys His Leu Asn 
                805                 810                 815     


Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys 
            820                 825                 830         


Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu 
        835                 840                 845             


Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val 
    850                 855                 860                 


Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr 
865                 870                 875                 880 


Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe 
                885                 890                 895 


<210>  20
<211>  2685
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Complete MTM25(+)_T2A_WTM1(-) nucleic amino acid sequence

<400>  20
atgttggggt ttgtgggtcg ggtggccgct gctccggcct ccggggcctt gcggagactc       60

accccttcag cgtcgctgcc cccagctcag ctcttactgc gggccgctcc gacggcggtc      120

catcctgtca gggactatgc ggcgcaatac ccctacgacg tgcccgacta cgccgtggat      180

gaaatgacca aaaagttcgg cacgctcacc attcacgaca ccgaaaaggc cgccgaattc      240

atggctgaga ggcccttcca gtgtcgaatc tgcatgcgta acttcagtgg caacaccggc      300

ctgaactgtc acatccgcac ccacaccggc gagaagcctt ttgcctgtga catttgtggg      360

aggaaatttg ccgaccgctc caacctgacc cgccatacca agatacacac gcatcccagg      420

gcacctattc ccaagccctt ccagtgtcga atctgcatgc gtaacttcag tcagtccggc      480

tccctgaccc gccacatccg cacccacacc ggcgagaagc cttttgcctg tgacatttgt      540

gggaggaaat ttgcccacaa gtccgcccgc gccgcccata ccaagataca cacgggatct      600

cagaagccct tccagtgtcg aatctgcatg cgtaacttca gtcgctccga ccacctgtcc      660

gcccacatcc gcacccacac cggcgagaag ccttttgcct gtgacatttg tgggaggaaa      720

tttgcccagc acggctccct ggcctcccat accaagatac acctgcgggg atcccagctg      780

gtgaagagcg agctggagga gaagaagtcc gagctgcggc acaagctgaa gtacgtgccc      840

cacgagtaca tcgagctgat cgagatcgcc aggaacagca cccaggaccg catcctggag      900

atgaaggtga tggagttctt catgaaggtg tacggctaca ggggaaagca cctgggcgga      960

agcagaaagc ctgacggcgc catctataca gtgggcagcc ccatcgatta cggcgtgatc     1020

gtggacacaa aggcctacag cggcggctac aatctgccta tcggccaggc cgacgagatg     1080

cagagatacg tgaaggagaa ccagacccgg aataagcaca tcaaccccaa cgagtggtgg     1140

aaggtgtacc ctagcagcgt gaccgagttc aagttcctgt tcgtgagcgg ccacttcaag     1200

ggcaactaca aggcccagct gaccaggctg aaccgcaaga ccaactgcaa tggcgccgtg     1260

ctgagcgtgg aggagctgct gatcggcggc gagatgatca aagccggcac cctgacactg     1320

gaggaggtgc ggcgcaagtt caacaacggc gagatcaact tcctcgagga agggagagga     1380

tctctgctta cttgcggcga tgtagaggaa aaccccggac ccatgctcgg tttcgtaggc     1440

cgcgtcgctg ccgcaccagc ttcaggtgca ctccgccgat tgacacccag cgcaagcctt     1500

cctcccgcac agttgttgct ccgagctgcc cccaccgccg ttcaccccgt gcgagattac     1560

gcagctcagt atccttatga tgtccctgat tatgctgtag acgagatgac aaagaaattt     1620

ggaactttga caatacatga tacagagaaa gctgcagaat tcatggccga acgccctttt     1680

caatgccgga tttgtatgag aaatttttct cttcctcacc acctggagca acatattagg     1740

acacatactg gggaaaaacc cttcgcttgc gatatctgcg gacgcaagtt cgctcggaat     1800

gcttcccgca ctcgacacac aaaaatccat acagggtccc agaagccatt ccaatgcagg     1860

atctgcatga gaaagttcgc atacacctac tctctctctg agcatactaa aattcacact     1920

ggggaaaaac catttcaatg cagaatatgt atgcgaaatt tctcccagag tgctaatcgg     1980

accactcata ttcgaacaca tacaggagaa aaacccttcg cttgcgatat ctgcggacga     2040

aagttcgctc ataggagtag cctccgccgc cacacaaaaa tccatcttcg cggatcccaa     2100

cttgttaaat cagaactcga agaaaaaaaa agcgagctac gccataaact caaatatgta     2160

cctcatgaat atattgaatt aattgaaatt gcaagaaata gtacacaaga tcgaattttg     2220

gaaatgaaag tcatggaatt ttttatgaaa gtatatggtt accgcggcaa acatcttgga     2280

ggatcaagga aaccagatgg ggcaatttac actgttggga gtcctataga ctacggggtc     2340

attgtcgata ccaaagctta ttctggaggg tataaccttc ccattggtca agctgatgaa     2400

atggagcgct atgtagaaga aaatcaaaca agagacaaac atcttaaccc taatgaatgg     2460

tggaaagtct atcccagttc tgttactgaa tttaaatttc tctttgtctc tggacatttt     2520

aaaggaaatt ataaagctca actcacaaga ttaaatcata taacaaattg taacggtgct     2580

gtactctcag tcgaagaact cctcattgga ggtgaaatga taaaggctgg aacactcacc     2640

ctcgaagaag ttcgccgaaa atttaataat ggggaaatta atttt                     2685


<210>  21
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  21

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A*

<400>  22

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  23
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  23

Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  24
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A*

<400>  24

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  25
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A

<400>  25

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  26
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A*

<400>  26

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A

<400>  27

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  28
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A*

<400>  28

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  29
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLS functional variant

<400>  29

Met Ser Val Leu Thr Pro Leu Leu Leu Arg Gly Leu Thr Gly Ser Ala 
1               5                   10                  15      


Arg Arg Leu Pro Val Pro Arg Ala Lys 
            20                  25  


<210>  30
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLS functional variant

<400>  30

Met Gln Thr Ala Gly Ala Leu Phe Ile Ser Pro Ala Leu Ile Arg Cys 
1               5                   10                  15      


Cys Thr Arg Gly Leu Ile Arg Pro Val Ser Ala Ser Phe Leu Asn Ser 
            20                  25                  30          


Pro Val Asn Ser Ser Lys Gln Pro Ser Tyr Ser Asn Phe Pro Leu Gln 
        35                  40                  45              


Val Ala Arg Arg Glu Phe Gln Thr Ser Val Val Ser Arg 
    50                  55                  60      


<210>  31
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLS functional variant

<400>  31

Met Leu Gly Phe Val Gly Arg Val Ala Ala Ala Pro Ala Ser Gly Ala 
1               5                   10                  15      


Leu Arg Arg Leu Thr Pro Ser Ala Ser Leu Pro Pro Ala Gln Leu Leu 
            20                  25                  30          


Leu Arg Ala Ala Pro Thr Ala Val His Pro Val Arg Asp Tyr Ala 
        35                  40                  45          


<210>  32
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLS functional variant

<400>  32

Met Leu Arg Ala Ala Ala Arg Phe Gly Pro Arg Leu Gly Arg Arg Leu 
1               5                   10                  15      


Leu 
    


<210>  33
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLS functional variant

<400>  33

Arg Ser Gly Ile Ile Lys Ala Ser Arg Val Leu Tyr Arg Gln Met 
1               5                   10                  15  


<210>  34
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HH (Hammerhead) ribozyme

<400>  34
ctgatgagtc cgtgaggacg aaacgagtaa gctcgtc                                37


<210>  35
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hepatitis delta virus (HDV) ribozyme

<400>  35
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg       60

aatgggac                                                                68


<210>  36
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak sequence

<400>  36
gccgccrcca ugg                                                          13


