                         SEQUENCE LISTING

<110>  NATIONAL RESEARCH COUNCIL OF CANADA
 
<120>  ANTIGEN-BINDING AGENTS THAT SPECIFICALLY BIND EPIDERMAL GROWTH 
       FACTOR RECEPTOR VARIANT III

<130>  16600-002-PCT

<150>  US 62/824,391
<151>  2019-03-27

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  1863
<212>  DNA
<213>  Homo sapiens

<400>  1
ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt gggcactttt       60

gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt ccttgggaat      120

ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac catccaggag      180

gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt ggaaaacctg      240

cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt cttatctaac      300

tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca ggaaatcctg      360

catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag catccagtgg      420

cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca gaaccacctg      480

ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg tgcaggagag      540

gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg gcgctgccgt      600

ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac aggcccccgg      660

gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa ggacacctgc      720

cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc cgagggcaaa      780

tacagctttg gtgccacctg cgtgaagaag tgtccccgta attatgtggt gacagatcac      840

ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga cggcgtccgc      900

aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg tattggtgaa      960

tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa ctgcacctcc     1020

atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt cacacatact     1080

cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat cacagggttt     1140

ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga gaacctagaa     1200

atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt cagcctgaac     1260

ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt gataatttca     1320

ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt tgggacctcc     1380

ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag     1440

gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag ggactgcgtc     1500

tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct tctggagggt     1560

gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga gtgcctgcct     1620

caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca gtgtgcccac     1680

tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg agaaaacaac     1740

accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca tccaaactgc     1800

acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg     1860

tcc                                                                   1863


<210>  2
<211>  621
<212>  PRT
<213>  Homo sapiens

<400>  2

Leu Glu Glu Lys Lys Val Cys Gln Gly Thr Ser Asn Lys Leu Thr Gln 
1               5                   10                  15      


Leu Gly Thr Phe Glu Asp His Phe Leu Ser Leu Gln Arg Met Phe Asn 
            20                  25                  30          


Asn Cys Glu Val Val Leu Gly Asn Leu Glu Ile Thr Tyr Val Gln Arg 
        35                  40                  45              


Asn Tyr Asp Leu Ser Phe Leu Lys Thr Ile Gln Glu Val Ala Gly Tyr 
    50                  55                  60                  


Val Leu Ile Ala Leu Asn Thr Val Glu Arg Ile Pro Leu Glu Asn Leu 
65                  70                  75                  80  


Gln Ile Ile Arg Gly Asn Met Tyr Tyr Glu Asn Ser Tyr Ala Leu Ala 
                85                  90                  95      


Val Leu Ser Asn Tyr Asp Ala Asn Lys Thr Gly Leu Lys Glu Leu Pro 
            100                 105                 110         


Met Arg Asn Leu Gln Glu Ile Leu His Gly Ala Val Arg Phe Ser Asn 
        115                 120                 125             


Asn Pro Ala Leu Cys Asn Val Glu Ser Ile Gln Trp Arg Asp Ile Val 
    130                 135                 140                 


Ser Ser Asp Phe Leu Ser Asn Met Ser Met Asp Phe Gln Asn His Leu 
145                 150                 155                 160 


Gly Ser Cys Gln Lys Cys Asp Pro Ser Cys Pro Asn Gly Ser Cys Trp 
                165                 170                 175     


Gly Ala Gly Glu Glu Asn Cys Gln Lys Leu Thr Lys Ile Ile Cys Ala 
            180                 185                 190         


Gln Gln Cys Ser Gly Arg Cys Arg Gly Lys Ser Pro Ser Asp Cys Cys 
        195                 200                 205             


His Asn Gln Cys Ala Ala Gly Cys Thr Gly Pro Arg Glu Ser Asp Cys 
    210                 215                 220                 


Leu Val Cys Arg Lys Phe Arg Asp Glu Ala Thr Cys Lys Asp Thr Cys 
225                 230                 235                 240 


Pro Pro Leu Met Leu Tyr Asn Pro Thr Thr Tyr Gln Met Asp Val Asn 
                245                 250                 255     


Pro Glu Gly Lys Tyr Ser Phe Gly Ala Thr Cys Val Lys Lys Cys Pro 
            260                 265                 270         


Arg Asn Tyr Val Val Thr Asp His Gly Ser Cys Val Arg Ala Cys Gly 
        275                 280                 285             


Ala Asp Ser Tyr Glu Met Glu Glu Asp Gly Val Arg Lys Cys Lys Lys 
    290                 295                 300                 


Cys Glu Gly Pro Cys Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu 
305                 310                 315                 320 


Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys 
                325                 330                 335     


Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe 
            340                 345                 350         


Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu 
        355                 360                 365             


Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln 
    370                 375                 380                 


Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu 
385                 390                 395                 400 


Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val 
                405                 410                 415     


Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile 
            420                 425                 430         


Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala 
        435                 440                 445             


Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr 
    450                 455                 460                 


Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln 
465                 470                 475                 480 


Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro 
                485                 490                 495     


Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val 
            500                 505                 510         


Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn 
        515                 520                 525             


Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn 
    530                 535                 540                 


Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His 
545                 550                 555                 560 


Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met 
                565                 570                 575     


Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val 
            580                 585                 590         


Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly 
        595                 600                 605             


Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser 
    610                 615                 620     


<210>  3
<211>  996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human EGFRvIII ectodomain cDNA sequence (nucleotides 1-996)

<400>  3
ctggaagaga agaaaggcaa ctacgtcgtg accgaccacg gcagctgtgt gcgggcttgt       60

ggcgccgata gctacgagat ggaagaggac ggcgtgcgga agtgcaagaa gtgcgagggc      120

ccctgccgga aagtgtgcaa cggcatcggc atcggagagt tcaaggacag cctgagcatc      180

aacgccacca acatcaagca cttcaagaac tgcaccagca tcagcggcga cctgcacatc      240

ctgcccgtgg cctttagagg cgacagcttc acccacaccc ccccactgga cccccaggaa      300

ctggacatcc tgaaaaccgt gaaagagatc accggctttc tgctgattca ggcctggccc      360

gagaaccgga cagacctgca cgccttcgag aacctggaaa tcatccgggg caggaccaag      420

cagcacggcc agttttctct ggccgtggtg tccctgaaca tcaccagcct gggcctgcgg      480

agcctgaaag aaatcagcga cggcgacgtg atcatctccg gcaacaagaa cctgtgctac      540

gccaacacca tcaactggaa gaagctgttc ggcacctccg gccagaaaac aaagatcatc      600

agcaaccggg gcgagaacag ctgcaaggcc acaggacaag tgtgccacgc cctgtgtagc      660

cctgagggct gttggggacc cgagcccaga gattgcgtgt cctgcagaaa cgtgtcccgg      720

ggcagagaat gcgtggacaa gtgcaacctg ctggaaggcg agccccgcga gttcgtggaa      780

aacagcgagt gcatccagtg ccaccccgag tgtctgcccc aggccatgaa cattacctgc      840

accggcagag gccccgacaa ctgtatccag tgcgcccact acatcgacgg cccccactgc      900

gtgaaaacct gtcctgctgg cgtgatggga gagaacaaca ccctcgtgtg gaagtacgcc      960

gacgccggcc atgtgtgcca cctgtgtcac cccaat                                996


<210>  4
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human EGFRvIII ectodomain amino acid sequence (amino acids 1-332)

<400>  4

Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys 
1               5                   10                  15      


Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp Gly Val 
            20                  25                  30          


Arg Lys Cys Lys Lys Cys Glu Gly Pro Cys Arg Lys Val Cys Asn Gly 
        35                  40                  45              


Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn 
    50                  55                  60                  


Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile 
65                  70                  75                  80  


Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu 
                85                  90                  95      


Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly 
            100                 105                 110         


Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala 
        115                 120                 125             


Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln 
    130                 135                 140                 


Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg 
145                 150                 155                 160 


Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys 
                165                 170                 175     


Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr 
            180                 185                 190         


Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys 
        195                 200                 205             


Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys 
    210                 215                 220                 


Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg 
225                 230                 235                 240 


Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg 
                245                 250                 255     


Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu 
            260                 265                 270         


Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys 
        275                 280                 285             


Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys 
    290                 295                 300                 


Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala 
305                 310                 315                 320 


Asp Ala Gly His Val Cys His Leu Cys His Pro Asn 
                325                 330         


<210>  5
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human EGFRvIII amino acid residues 15 to 37

<400>  5

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  6
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 light chain variable region

<400>  6

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRL1

<400>  7

Lys Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRL2

<400>  8

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRL3

<400>  9

Trp Gln Gly Thr His Phe Pro Gln Thr 
1               5                   


<210>  10
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 heavy chain variable region

<400>  10

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRH1

<400>  11

Asn Tyr Gly Met Asn 
1               5   


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRH2

<400>  12

Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CDRH3

<400>  13

Gly Pro Asn Phe Asp Val 
1               5       


<210>  14
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 light chain variable region

<400>  14

Glu Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ala Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Val Ser Ser Ser Ile Ser Ser Ser 
            20                  25                  30          


Asn Leu His Trp Tyr Gln Gln Lys Ser Glu Thr Ser Pro Lys Pro Trp 
        35                  40                  45              


Ile Tyr Gly Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Tyr Pro 
                85                  90                  95      


Leu Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105             


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRL1

<400>  15

Ser Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His 
1               5                   10          


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRL2

<400>  16

Gly Thr Ser Asn Leu Ala Ser 
1               5           


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRL3

<400>  17

Gln Gln Trp Ser Ser Tyr Pro Leu Thr 
1               5                   


<210>  18
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 heavy chain variable region

<400>  18

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala 
        115                 


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRH1

<400>  19

Asp Tyr Asn Met Asp 
1               5   


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRH2

<400>  20

Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CDRH3

<400>  21

Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr 
1               5                   10  


<210>  22
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 light chain variable region

<400>  22

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  23
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRL1

<400>  23

Arg Ser Ser Gln Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRL2

<400>  24

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRL3

<400>  25

Ser Gln Ser Thr His Val Pro Trp Thr 
1               5                   


<210>  26
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 heavy chain variable region

<400>  26

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala 
        115     


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRH1

<400>  27

Ser Gly Tyr Ser Trp His 
1               5       


<210>  28
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRH2

<400>  28

Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CDRH3

<400>  29

Ser Pro Tyr Phe Asp Val 
1               5       


<210>  30
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CAR construct amino acid sequence (with linker and CD8 hinge)

<400>  30

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
145                 150                 155                 160 


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
                165                 170                 175     


Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
            180                 185                 190         


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
        195                 200                 205             


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
225                 230                 235                 240 


Cys Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro 
            260                 265                 270         


Pro Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr Phe Ser 
        275                 280                 285             


Ser Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro 
    290                 295                 300                 


Val Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro 
305                 310                 315                 320 


Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly 
                325                 330                 335     


Leu Asp Phe Ala 
            340 


<210>  31
<211>  1052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5B7 CAR construct nucleotide sequence

<400>  31
gacagaagac ctaggacaga tccagttggt gcagtctgga cctgagctga agaagcctgg       60

agagacagtc atgatctcct gcaaggcttc tgggtattcc ttcacaaact atggaatgaa      120

ctgggtgaag caggctccag aaaaggattt aaagtggatg ggctggataa acacctacac      180

tggagagtca agatatgttg atgaattcaa gggacggttt gccttctctt tggaaacctc      240

tgttagtatt gtctatttga agatcaacaa cctcaaaaat gaggacatgg ctacatattt      300

ctgtgcaaga gggcctaatt tcgatgtctg gggcacaggg accacggtca ctgtctcctc      360

agccaaaaca acagccccat ccgtctatcc cctggcccct ggaagcttgg gaggtaccgg      420

cggaagtgga ggcggaggat ctggcggcgg aggatccgat gttgtgatga cccagactcc      480

actcactttg tcggttacca ttggacaacc agcctccatc tcttgcaagt caagtcagag      540

cctcttagat agtgatggaa agacatattt gaattggttg ttacagaggc caggccagtc      600

tccaaagcgc ctaatctatc tggtgtctaa actggactct ggagtccctg acaggttcac      660

tggcagtgga tcagggacag atttcacact gaaaatcagc agagtggagg ctgaggattt      720

gggagtttat tattgctggc aaggtacaca ttttcctcag acgttcggtg gaggcaccaa      780

gctggaaatc aaacgggctg atgctgcacc aactgtatcc atcttcccac catccagtaa      840

gcttggggtc atcagcaact cggtgatgta cttcagttct gtcgtgccag tccttcagaa      900

agtgaactct actactacca agccagtgct gcgaactccc tcacctgtgc accctaccgg      960

gacatctcag ccccaaagac cagaagattg tcggccccgt ggctcagtga aggggaccgg     1020

attggacttc gccccttggg tcttcggtag gg                                   1052


<210>  32
<211>  548
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CAR construct with CD28 intracellular transduction domain 
       (ITD) and CD3zeta ITD

<400>  32

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro 
            20                  25                  30          


Gly Glu Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr 
        35                  40                  45              


Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys 
    50                  55                  60                  


Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp 
65                  70                  75                  80  


Glu Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile 
                85                  90                  95      


Val Tyr Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr 
            100                 105                 110         


Phe Cys Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr 
        115                 120                 125             


Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu 
    130                 135                 140                 


Ala Pro Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Thr 
                165                 170                 175     


Leu Ser Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser 
            180                 185                 190         


Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu 
        195                 200                 205             


Gln Arg Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys 
    210                 215                 220                 


Leu Asp Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr 
225                 230                 235                 240 


Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val 
                245                 250                 255     


Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly 
            260                 265                 270         


Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
        275                 280                 285             


Phe Pro Pro Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr 
    290                 295                 300                 


Phe Ser Ser Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr 
305                 310                 315                 320 


Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser 
                325                 330                 335     


Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly 
            340                 345                 350         


Thr Gly Leu Asp Phe Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val 
        355                 360                 365             


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
    370                 375                 380                 


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
385                 390                 395                 400 


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
                405                 410                 415     


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser 
            420                 425                 430         


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
        435                 440                 445             


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
    450                 455                 460                 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
465                 470                 475                 480 


Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                485                 490                 495     


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            500                 505                 510         


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        515                 520                 525             


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    530                 535                 540                 


Pro Arg Leu Glu 
545             


<210>  33
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CAR construct amino acid sequence (with linker and CD8 
       hinge)

<400>  33

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro 
145                 150                 155                 160 


Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser 
                165                 170                 175     


Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys 
            180                 185                 190         


Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
    210                 215                 220                 


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro 
            260                 265                 270         


Pro Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr Phe Ser 
        275                 280                 285             


Ser Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro 
    290                 295                 300                 


Val Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro 
305                 310                 315                 320 


Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly 
                325                 330                 335     


Leu Asp Phe Ala 
            340 


<210>  34
<211>  548
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CAR construct with CD28 ITD and CD3zeta ITD

<400>  34

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
            20                  25                  30          


Gly Ser Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
        35                  40                  45              


Asp Tyr Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu 
    50                  55                  60                  


Trp Ile Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln 
65                  70                  75                  80  


Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 
                85                  90                  95      


Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser 
    130                 135                 140                 


Val Tyr Pro Leu Ala Pro Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln 
                165                 170                 175     


Ser Pro Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr 
            180                 185                 190         


Cys Ser Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln 
        195                 200                 205             


Gln Lys Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn 
    210                 215                 220                 


Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr 
225                 230                 235                 240 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
                245                 250                 255     


Tyr Tyr Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly 
            260                 265                 270         


Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
        275                 280                 285             


Phe Pro Pro Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr 
    290                 295                 300                 


Phe Ser Ser Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr 
305                 310                 315                 320 


Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser 
                325                 330                 335     


Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly 
            340                 345                 350         


Thr Gly Leu Asp Phe Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val 
        355                 360                 365             


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
    370                 375                 380                 


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
385                 390                 395                 400 


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
                405                 410                 415     


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser 
            420                 425                 430         


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
        435                 440                 445             


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
    450                 455                 460                 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
465                 470                 475                 480 


Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                485                 490                 495     


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            500                 505                 510         


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        515                 520                 525             


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    530                 535                 540                 


Pro Arg Leu Glu 
545             


<210>  35
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CAR construct amino acid sequence (with linker and CD8 hinge)

<400>  35

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Leu Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
145                 150                 155                 160 


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu 
                165                 170                 175     


Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro 
            180                 185                 190         


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
        195                 200                 205             


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys 
225                 230                 235                 240 


Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
            260                 265                 270         


Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr Phe Ser Ser 
        275                 280                 285             


Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro Val 
    290                 295                 300                 


Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln 
305                 310                 315                 320 


Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu 
                325                 330                 335     


Asp Phe Ala 
            


<210>  36
<211>  547
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 CAR construct with CD28 ITD and CD3zeta ITD

<400>  36

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro 
            20                  25                  30          


Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr 
        35                  40                  45              


Ser Gly Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu 
    50                  55                  60                  


Glu Trp Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro 
65                  70                  75                  80  


Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln 
                85                  90                  95      


Phe Phe Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr 
        115                 120                 125             


Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
    130                 135                 140                 


Ala Pro Gly Ser Leu Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
                165                 170                 175     


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
            180                 185                 190         


Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
        195                 200                 205             


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
    210                 215                 220                 


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
225                 230                 235                 240 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
                245                 250                 255     


Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr 
            260                 265                 270         


Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe 
        275                 280                 285             


Pro Pro Ser Ser Lys Leu Gly Val Ile Ser Asn Ser Val Met Tyr Phe 
    290                 295                 300                 


Ser Ser Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys 
305                 310                 315                 320 


Pro Val Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln 
                325                 330                 335     


Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr 
            340                 345                 350         


Gly Leu Asp Phe Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
        355                 360                 365             


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
    370                 375                 380                 


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
385                 390                 395                 400 


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
                405                 410                 415     


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Leu 
            420                 425                 430         


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
        435                 440                 445             


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
    450                 455                 460                 


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
465                 470                 475                 480 


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
                485                 490                 495     


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
            500                 505                 510         


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
        515                 520                 525             


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
    530                 535                 540                 


Arg Leu Glu 
545         


<210>  37
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence for modular CAR vector (without filler/insert)

<400>  37
atgctcaggc tgctcttggc tctcaactta ttcccttcaa ttcaagtaac aggagggtct       60

tcgagaagac ctccttctaa gcccttttgg gtgctggtgg tggttggtgg agtcctggct      120

tgctatagct tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc      180

aggctcctgc acagtgacta catgaacatg actcccaggc ggcccggacc cacccgcaag      240

cattaccagc cctatgcccc accacgcgac ttcgcagcct atcgctccgc tagcctgaga      300

gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat      360

aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg      420

gaccctgaga tggggggaaa gccgcagaga aggaagaacc ctcaggaagg cctgtacaat      480

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      540

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      600

tacgacgccc ttcacatgca ggccctgccc cctcgcctcg ag                         642


<210>  38
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5B7 Heavy chain variable region nucleotide sequence

<400>  38
cagatccagt tggtgcagtc tggacctgag ctgaagaagc ctggagagac agtcatgatc       60

tcctgcaagg cttctgggta ttccttcaca aactatggaa tgaactgggt gaagcaggct      120

ccagaaaagg atttaaagtg gatgggctgg ataaacacct acactggaga gtcaagatat      180

gttgatgaat tcaagggacg gtttgccttc tctttggaaa cctctgttag tattgtctat      240

ttgaagatca acaacctcaa aaatgaggac atggctacat atttctgtgc aagagggcct      300

aatttcgatg tctggggcac agggaccacg gtcaccgtct cctca                      345


<210>  39
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5B7 Light chain variable region nucleotide sequence

<400>  39
gatgttgtga tgacccagac tccactcact ttgtcggtta ccattggaca accagcctcc       60

atctcttgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaaag cgcctaatct atctggtgtc taaactggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc      240

agcagagtgg aggctgagga tttgggagtt tattattgct ggcaaggtac acattttcct      300

cagacgttcg gtggaggcac caagctggaa atcaaa                                336


<210>  40
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3D12 Heavy chain variable region nucleotide sequence

<400>  40
gaggtccagc tgcaacagtc tggacctgag ctggtgaagc ctgggtcttc agtgaggata       60

tcctgcaaag cttctggata cacattcact gactacaaca tggactgggt gaagcagagc      120

catggaaaga gccttgagtg gattggaact attaatccta acaatggtgg tactagctac      180

aaccagaagt tcaagggcaa ggccacattg actgttgaca agtcctccag cacagcctac      240

atggaactcc gcagcctgac atctgaggac tctgcagtct attactgtgc aagagtgaga      300

cagctcgggc tgtggtttgc ttactggggc caagggactc tggtcactgt ctctgca         357


<210>  41
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3D12 Light chain variable region nucleotide sequence

<400>  41
gaaattgtgc tcacccagtc tccagcactc atggctgcat ctccagggga gaaggtcacc       60

atcacctgca gtgtcagctc aagtataagt tccagcaact tgcactggta ccagcagaag      120

tcagaaacct cccccaaacc ctggatttat ggcacatcca acctggcttc tggagtccct      180

gttcgcttca gtggcagtgg atctgggacc tcttattctc tcacaatcag cagcatggag      240

gctgaagatg ctgccactta ttactgtcaa cagtggagta gttacccact cacgttcggc      300

tcggggacaa agttggaaat aaaa                                             324


<210>  42
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1D2 Heavy chain variable nucleotide sequence

<400>  42
gatgtgcagc ttcaggagtc aggacctgac ctggtgaaac cttctcagtc actttcactc       60

acctgcactg tcactggcta ctccatcacc agtggttata gctggcactg gatccggcag      120

tttccaggaa acaaactgga atggatgggc tacatacact acagtggtag cactaactac      180

aacccatctc tcaaaagtcg aatctctatc actcgagaca catccaagaa ccagttcttc      240

ctgcagttga gttctgtgac tactgaggac actgccacat attactgtac aagaagcccg      300

tacttcgatg tctggggcac agggaccacg gtcaccgtct cctca                      345


<210>  43
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1D2 Light chain variable region nucleotide sequence

<400>  43
gatgttgtga tgacccaaac tccactctcc ctgcctgtca gtcttggcga tcaagcctcc       60

atctcttgca gatctagtca gagccttgtg cacagtgatg gaaacaccta tttacattgg      120

tacctgcaga agccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg aggctgagga tctgggagtt tatttctgct ctcaaagtac acatgttccg      300

tggacgttcg gtggaggcac caagctggaa atcaaa                                336


<210>  44
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 scFV

<400>  44

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
145                 150                 155                 160 


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
                165                 170                 175     


Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
            180                 185                 190         


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
        195                 200                 205             


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
225                 230                 235                 240 


Cys Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro 
            260                 265                 270         


Pro Ser Ser Lys Leu Gly 
        275             


<210>  45
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 scFV

<400>  45

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro 
145                 150                 155                 160 


Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser 
                165                 170                 175     


Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys 
            180                 185                 190         


Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
    210                 215                 220                 


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro 
            260                 265                 270         


Pro Ser Ser Lys Leu Gly 
        275             


<210>  46
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 scFV

<400>  46

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Leu Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
145                 150                 155                 160 


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu 
                165                 170                 175     


Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro 
            180                 185                 190         


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
        195                 200                 205             


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys 
225                 230                 235                 240 


Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
            260                 265                 270         


Ser Ser Lys Leu Gly 
        275         


<210>  47
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  47

Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  


<210>  48
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Ser15 to Ala 
       mutation

<400>  48

Ala Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  49
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Cys16 to Ala 
       mutation

<400>  49

Ser Ala Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  50
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Val17 to Ala 
       mutation

<400>  50

Ser Cys Ala Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  51
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Arg18 to Ala 
       mutation

<400>  51

Ser Cys Val Ala Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  52
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Cys20 to Ala 
       mutation

<400>  52

Ser Cys Val Arg Ala Ala Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  53
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Gly21 to Ala 
       mutation

<400>  53

Ser Cys Val Arg Ala Cys Ala Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  54
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Asp23 to Ala 
       mutation

<400>  54

Ser Cys Val Arg Ala Cys Gly Ala Ala Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  55
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Ser24 to Ala 
       mutation

<400>  55

Ser Cys Val Arg Ala Cys Gly Ala Asp Ala Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  56
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Tyr25 to Ala 
       mutation

<400>  56

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Ala Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  57
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Glu26 to Ala 
       mutation

<400>  57

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Ala Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  58
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Met27 to Ala 
       mutation

<400>  58

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Ala Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  59
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Glu28 to Ala 
       mutation

<400>  59

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Ala Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  60
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Glu29 to Ala 
       mutation

<400>  60

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Ala Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  61
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Asp30 to Ala 
       mutation

<400>  61

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Ala 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Lys 
            20              


<210>  62
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Gly31 to Ala 
       mutation

<400>  62

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Ala Val Arg Lys Cys Lys Lys 
            20              


<210>  63
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Val32 to Ala 
       mutation

<400>  63

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Ala Arg Lys Cys Lys Lys 
            20              


<210>  64
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Arg33 to Ala 
       mutation

<400>  64

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Ala Lys Cys Lys Lys 
            20              


<210>  65
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Lys34 to Ala 
       mutation

<400>  65

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Ala Cys Lys Lys 
            20              


<210>  66
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Cys35 to Ala 
       mutation

<400>  66

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Ala Lys Lys 
            20              


<210>  67
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Lys36 to Ala 
       mutation

<400>  67

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Ala Lys 
            20              


<210>  68
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 15 to 37 of EGFRvIII, with Lys37 to Ala 
       mutation

<400>  68

Ser Cys Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp 
1               5                   10                  15      


Gly Val Arg Lys Cys Lys Ala 
            20              


<210>  69
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 1 to 76 of EGFRvIII

<400>  69

Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys 
1               5                   10                  15      


Val Arg Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp Gly Val 
            20                  25                  30          


Arg Lys Cys Lys Lys Cys Glu Gly Pro Cys Arg Lys Val Cys Asn Gly 
        35                  40                  45              


Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn 
    50                  55                  60                  


Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly 
65                  70                  75      


<210>  70
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 3 to 37 of EGFRvIII

<400>  70

Glu Lys Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys Val Arg 
1               5                   10                  15      


Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu Asp Gly Val Arg Lys 
            20                  25                  30          


Cys Lys Lys 
        35  


<210>  71
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid residues 1 to 18 of EGFRvIII

<400>  71

Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys 
1               5                   10                  15      


Val Arg 
        


<210>  72
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 Shorter scFv

<400>  72

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val 
    130                 135                 140                 


Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu 
145                 150                 155                 160 


Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro 
                165                 170                 175     


Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser 
            180                 185                 190         


Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys 
    210                 215                 220                 


Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys 
            


<210>  73
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 scFv consensus


<220>
<221>  MISC_FEATURE
<222>  (116)..(116)
<223>  Linker

<400>  73

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Xaa Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
        115                 120                 125             


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
    130                 135                 140                 


Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
145                 150                 155                 160 


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
                165                 170                 175     


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
            180                 185                 190         


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
        195                 200                 205             


Cys Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys 
    210                 215                 220                 


Leu Glu Ile 
225         


<210>  74
<211>  479
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5B7 CAR consensus


<220>
<221>  MISC_FEATURE
<222>  (116)..(116)
<223>  Linker

<400>  74

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Glu Lys Asp Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Arg Tyr Val Asp Glu Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Val Ser Ile Val Tyr 
65                  70                  75                  80  


Leu Lys Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Pro Asn Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Xaa Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
        115                 120                 125             


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
    130                 135                 140                 


Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
145                 150                 155                 160 


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
                165                 170                 175     


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
            180                 185                 190         


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
        195                 200                 205             


Cys Trp Gln Gly Thr His Phe Pro Gln Thr Phe Gly Gly Gly Thr Lys 
    210                 215                 220                 


Leu Glu Ile Val Ile Ser Asn Ser Val Met Tyr Phe Ser Ser Val Val 
225                 230                 235                 240 


Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro Val Leu Arg 
                245                 250                 255     


Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln Arg Pro 
            260                 265                 270         


Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu Asp Phe 
        275                 280                 285             


Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
    290                 295                 300                 


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
305                 310                 315                 320 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
                325                 330                 335     


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            340                 345                 350         


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Leu Arg Val Lys Phe 
        355                 360                 365             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    370                 375                 380                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
385                 390                 395                 400 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg 
                405                 410                 415     


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
            420                 425                 430         


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
        435                 440                 445             


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
    450                 455                 460                 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu 
465                 470                 475                 


<210>  75
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12  Shorter scFV

<400>  75

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Gly Thr Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser Val 
145                 150                 155                 160 


Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys Ser 
                165                 170                 175     


Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala Ser 
            180                 185                 190         


Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
        195                 200                 205             


Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys 
            


<210>  76
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12  scFV consensus


<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  Linker

<400>  76

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Xaa Glu Ile Val Leu Thr Gln Ser Pro 
        115                 120                 125             


Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser 
    130                 135                 140                 


Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys 
145                 150                 155                 160 


Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala 
                165                 170                 175     


Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
            180                 185                 190         


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
        195                 200                 205             


Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys 
    210                 215                 220                 


Leu Glu Ile Lys 
225             


<210>  77
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 CAR consensus


<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  Linker

<400>  77

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Xaa Glu Ile Val Leu Thr Gln Ser Pro 
        115                 120                 125             


Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser 
    130                 135                 140                 


Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys 
145                 150                 155                 160 


Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala 
                165                 170                 175     


Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
            180                 185                 190         


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
        195                 200                 205             


Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys 
    210                 215                 220                 


Leu Glu Ile Lys Val Ile Ser Asn Ser Val Met Tyr Phe Ser Ser Val 
225                 230                 235                 240 


Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro Val Leu 
                245                 250                 255     


Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln Arg 
            260                 265                 270         


Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu Asp 
        275                 280                 285             


Phe Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
    290                 295                 300                 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
305                 310                 315                 320 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
                325                 330                 335     


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            340                 345                 350         


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Leu Arg Val Lys 
        355                 360                 365             


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
    370                 375                 380                 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
385                 390                 395                 400 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg 
                405                 410                 415     


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
            420                 425                 430         


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
        435                 440                 445             


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
    450                 455                 460                 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu 
465                 470                 475                 480 


<210>  78
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 Shorter scFV

<400>  78

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Gly Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro 
    130                 135                 140                 


Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser 
145                 150                 155                 160 


Leu Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys 
                165                 170                 175     


Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe 
    210                 215                 220                 


Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Ile Lys 
                


<210>  79
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2 Consensus


<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  Linker

<400>  79

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Xaa Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
        115                 120                 125             


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
    130                 135                 140                 


Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
145                 150                 155                 160 


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
                165                 170                 175     


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
            180                 185                 190         


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
        195                 200                 205             


Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr 
    210                 215                 220                 


Lys Leu Glu Ile Lys 
225                 


<210>  80
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1D2  CAR Consensus


<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  Linker

<400>  80

Asp Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Ser Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile His Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Pro Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Ala Xaa Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
        115                 120                 125             


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
    130                 135                 140                 


Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
145                 150                 155                 160 


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
                165                 170                 175     


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
            180                 185                 190         


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
        195                 200                 205             


Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr 
    210                 215                 220                 


Lys Leu Glu Ile Lys Val Ile Ser Asn Ser Val Met Tyr Phe Ser Ser 
225                 230                 235                 240 


Val Val Pro Val Leu Gln Lys Val Asn Ser Thr Thr Thr Lys Pro Val 
                245                 250                 255     


Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln 
            260                 265                 270         


Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu 
        275                 280                 285             


Asp Phe Ala Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
    290                 295                 300                 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
305                 310                 315                 320 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
                325                 330                 335     


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
            340                 345                 350         


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Leu Arg Val 
        355                 360                 365             


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
    370                 375                 380                 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
385                 390                 395                 400 


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln 
                405                 410                 415     


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
            420                 425                 430         


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
        435                 440                 445             


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
    450                 455                 460                 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu 
465                 470                 475                 480 


Glu 
    


<210>  81
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3GGGGS-flexible linker and human CD8 hinge

<400>  81

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Thr 
1               5                   10                  15      


Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
            20                  25                  30          


Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
        35                  40                  45              


Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
    50                  55                  60  


<210>  82
<211>  531
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D12 bi-specific T cell engager

<400>  82

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Arg Gln Leu Gly Leu Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Leu Gly Gly Thr Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro 
145                 150                 155                 160 


Ala Leu Met Ala Ala Ser Pro Gly Glu Lys Val Thr Ile Thr Cys Ser 
                165                 170                 175     


Val Ser Ser Ser Ile Ser Ser Ser Asn Leu His Trp Tyr Gln Gln Lys 
            180                 185                 190         


Ser Glu Thr Ser Pro Lys Pro Trp Ile Tyr Gly Thr Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
    210                 215                 220                 


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Gln Gln Trp Ser Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro 
            260                 265                 270         


Pro Ser Ser Lys Leu Gly Asp Leu Gly Gly Gly Gly Ser Arg Asp Asp 
        275                 280                 285             


Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
    290                 295                 300                 


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr 
305                 310                 315                 320 


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
                325                 330                 335     


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
            340                 345                 350         


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
        355                 360                 365             


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
    370                 375                 380                 


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
385                 390                 395                 400 


Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly 
                405                 410                 415     


Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser 
            420                 425                 430         


Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
        435                 440                 445             


Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser 
    450                 455                 460                 


Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser 
465                 470                 475                 480 


Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
                485                 490                 495     


Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
            500                 505                 510         


Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
        515                 520                 525             


Glu Leu Lys 
    530     


<210>  83
<211>  64
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker of the L17-CD8 construct

<400>  83

Pro Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Gly Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
            20                  25                  30          


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
        35                  40                  45              


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
    50                  55                  60                  


<210>  84
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker of the 45CD8h construct

<400>  84

Pro Leu Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
1               5                   10                  15      


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            20                  25                  30          


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45          


<210>  85
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker of the 35CD8h construct

<400>  85

Pro Leu Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
1               5                   10                  15      


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            20                  25                  30          


Asp Phe Ala Cys Asp 
        35          


<210>  86
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker of the 15CD8h construct

<400>  86

Pro Leu Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
1               5                   10                  15      


Asp 
    


