                         SEQUENCE LISTING

<110>  Research Institute At Nationwide Children's Hospital
 
<120>  GENERATION OF CHIMERIC ANTIGEN RECEPTOR (CAR)-PRIMARY NK CELLS 
       FOR CANCER IMMUNOTHERAPY USING A COMBINATION OF CAS9/RNP AND AAV 
       VIRUSES

<130>  10935-004WO1

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1
gattggtgac agaaaagccc catccttagg                                        30


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2
ttatctgtcc cctccacccc acagtggggc                                        30


<210>  3
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3
gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg       60

tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg      120

agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc      180

ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt      240

tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc      300


<210>  4
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4
gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc       60

cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc      120

cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca      180

tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc      240

cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc      300


<210>  5
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5
gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg       60

tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg      120

agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc      180

ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt      240

tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc      300

tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag      360

tttggaaaaa caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt      420

ctagtcccca atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt      480

agccagcccc gtcctggcag                                                  500


<210>  6
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6
tcccttttcc ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc       60

cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt      120

ttctggacaa ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc      180

tttctttgcc tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt      240

tgggcagctc ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt      300

catggcatct tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct      360

atgtccactt caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga      420

ccaccttata ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc      480

cctccacccc acagtggggc                                                  500


<210>  7
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7
gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg       60

tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg      120

agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc      180

ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt      240

tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc      300

tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag      360

tttggaaaaa caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt      420

ctagtcccca atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt      480

agccagcccc gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc      540

tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta ctccctttct      600

ctttctccat ccttctttcc ttaaagagtc cccagtgcta tctgggacat attcctccgc      660

ccagagcagg gtcccgcttc cctaaggccc tgctctgggc ttctgggttt gagtccttgg      720

caagcccagg agaggcgctc aggcttccct gtcccccttc ctcgtccacc atctcatgcc      780

cctggctctc ctgccccttc cctacagggg ttcctggctc tgctcttcag actgagcccc      840

gttcccctgc atccccgttc ccctgcatcc cccttcccct gcatccccca gaggccccag      900

gccacctact tggcctggac cccacgagag gccaccccag ccctgtctac caggctgcct      960

tttgggtgga ttctcctcca actgtggggt gactgcttgg                           1000


<210>  8
<211>  804
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8
tgctttctct gacctgcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt       60

ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt      120

cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc      180

cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg      240

tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct      300

gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct      360

tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc      420

gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc      480

ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt      540

catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc      600

ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc      660

ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct      720

gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct      780

gtcccctcca ccccacagtg gggc                                             804


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  9
ccaatcctgt ccctagtggc ccc                                               23


<210>  10
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  10
atcgatcgca ggcgcaatct tcgcatttct tttttccag                              39


<210>  11
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11
cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct       60

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc      120

attgtctgag taggtgtcat tctattc                                          147


<210>  12
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12
gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg       60

cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc      120

ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc      180

gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc      240

gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg      300

atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc      360

gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg      420

cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc      480

ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag      540

gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac      600

atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc      660

gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaa                   708


<210>  13
<211>  986
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  13
ttatctgtcc cctccacccc acagtggggc cactagggac agcgatcggg tacatcgatc       60

gcaggcgcaa tcttcgcatt tcttttttcc aggtgagcaa gggcgaggag gataacatgg      120

ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg      180

agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc      240

tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca      300

tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt      360

ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga      420

ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg      480

gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct      540

cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga      600

agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc      660

ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg      720

aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca      780

tggacgagct gtacaagtaa cgcggccgcc ctcgactgtg ccttctagtt gccagccatc      840

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct      900

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattcgatt      960

ggtgacagaa aagccccatc cttagg                                           986


<210>  14
<211>  1526
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  14
gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc       60

cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc      120

cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca      180

tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc      240

cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc      300

cactagggac agcgatcggg tacatcgatc gcaggcgcaa tcttcgcatt tcttttttcc      360

aggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg      420

tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc      480

gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct      540

tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc      600

ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg      660

tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg      720

gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa      780

tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg      840

ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg      900

aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca      960

acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac     1020

gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa cgcggccgcc     1080

ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt     1140

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca     1200

ttgtctgagt aggtgtcatt ctattcgatt ggtgacagaa aagccccatc cttaggcctc     1260

ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta     1320

tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt     1380

tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga     1440

agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga     1500

acctgagctg ctctgacgcg gctgtc                                          1526


<210>  15
<211>  1926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  15
tcccttttcc ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc       60

cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt      120

ttctggacaa ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc      180

tttctttgcc tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt      240

tgggcagctc ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt      300

catggcatct tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct      360

atgtccactt caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga      420

ccaccttata ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc      480

cctccacccc acagtggggc cactagggac agcgatcggg tacatcgatc gcaggcgcaa      540

tcttcgcatt tcttttttcc aggtgagcaa gggcgaggag gataacatgg ccatcatcaa      600

ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat      660

cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac      720

caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc      780

caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga      840

gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca      900

ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt      960

cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg     1020

gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga     1080

cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct     1140

gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac     1200

catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct     1260

gtacaagtaa cgcggccgcc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc     1320

ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa     1380

aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattcgatt ggtgacagaa     1440

aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta acccccacct     1500

cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc atctctctcc     1560

ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg gagggagagc     1620

ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc agtggccacc     1680

ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt gcgtttcact     1740

gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg gaaaaacaaa     1800

atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag tccccaattt     1860

atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc agccccgtcc     1920

tggcag                                                                1926


<210>  16
<211>  2730
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  16
tgctttctct gacctgcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt       60

ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt      120

cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc      180

cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg      240

tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct      300

gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct      360

tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc      420

gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc      480

ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt      540

catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc      600

ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc      660

ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct      720

gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct      780

gtcccctcca ccccacagtg gggccactag ggacagcgat cgggtacatc gatcgcaggc      840

gcaatcttcg catttctttt ttccaggtga gcaagggcga ggaggataac atggccatca      900

tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg      960

agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg     1020

tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg     1080

gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc     1140

ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga     1200

cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca     1260

acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg     1320

agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga     1380

aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc     1440

agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact     1500

acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg     1560

agctgtacaa gtaacgcggc cgccctcgac tgtgccttct agttgccagc catctgttgt     1620

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     1680

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc gattggtgac     1740

agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc     1800

acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg agccatctct     1860

ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc ctgggaggga     1920

gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc     1980

caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt     2040

cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa     2100

caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca     2160

atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc     2220

gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa     2280

tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat     2340

ccttctttcc ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg     2400

gtcccgcttc cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg     2460

agaggcgctc aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc     2520

ctgccccttc cctacagggg ttcctggctc tgctcttcag actgagcccc gttcccctgc     2580

atccccgttc ccctgcatcc cccttcccct gcatccccca gaggccccag gccacctact     2640

tggcctggac cccacgagag gccaccccag ccctgtctac caggctgcct tttgggtgga     2700

ttctcctcca actgtggggt gactgcttgg                                      2730


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  17
ggggccacta gggacaggat                                                   20


