                         SEQUENCE LISTING

<110>  INTELLISTEM TECHNOLOGIES INC.
 
<120>  OVEREXPRESSION OF IMMUNOPROTEASOME IN STEM CELLS FOR GENERATING 
       ANTIGEN-PRESENTING CELLS

<130>  10207151-1PCT

<150>  US 62/835,678
<151>  2019-04-18

<150>  US 62/912,331
<151>  2019-10-08

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  2434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Viral transduction construct

<400>  1
tcgagacgcg ggcggccacc atgctgcggg cgggagcacc aaccggggac ttaccccggg       60

cgggagaagt ccacaccggg accaccatca tggcagtgga gtttgacggg ggcgttgtga      120

tgggttctga ttcccgagtg tctgcaggcg aggcggtggt gaaccgagtg tttgacaagc      180

tgtccccgct gcacgagcgc atctactgtg cactctctgg ttcagctgct gatgcccaag      240

ccgtggccga catggccgcc taccagctgg agctccatgg gatagaactg gaggaacctc      300

cacttgtttt ggctgctgca aatgtggtga gaaatatcag ctataaatat cgagaggact      360

tgtctgcaca tctcatggta gctggctggg accaacgtga aggaggtcag gtatatggaa      420

ccctgggagg aatgctgact cgacagcctt ttgccattgg tggctccggc agcaccttta      480

tctatggtta tgtggatgca gcatataagc caggcatgtc tcccgaggag tgcaggcgct      540

tcaccacaga cgctattgct ctggccatga gccgggatgg ctcaagcggg ggtgtcatct      600

acctggtcac tattacagct gccggtgtgg accatcgagt catcttgggc aatgaactgc      660

caaaattcta tgatgaggaa gggcgcggga gcctgctgac atgtggggac gtagaagaaa      720

acccagggcc aatgctgaag ccagccctgg agccccgagg gggcttctcc ttcgagaact      780

gccaaagaaa tgcatcattg gaacgcgtcc tcccggggct caaggtccct cacgcacgca      840

agaccgggac caccatcgcg ggcctggtgt tccaagacgg ggtcattctg ggcgccgata      900

cgcgagccac taacgattcg gtcgtggcgg acaagagctg cgagaagatc cacttcatcg      960

cccccaaaat ctactgctgt ggggctggag tagccgcgga cgccgagatg accacacgga     1020

tggtggcgtc caagatggag ctacacgcgt tatctacggg ccgcgagccc cgcgtggcca     1080

cggtcactcg catcctgcgc cagacgctct tcaggtacca gggccacgtg ggtgcatcgc     1140

tgatcgtggg cggcgtagac ctgactggac cgcagctcta cggtgtgcat ccccatggct     1200

cctacagccg tctgcccttc acagccctgg gctctggtca ggacgcggcc ctggcggtgc     1260

tagaagaccg gttccagccg aacatgacgc tggaggctgc tcaggggctg ctggtggaag     1320

ccgtcaccgc cgggatcttg ggtgacctgg gctccggggg caatgtggac gcatgtgtga     1380

tcacaaagac tggcgccaag ctgctgcgga cactgagctc acccacagag cccgtgaaga     1440

ggtctggccg ctaccacttt gtgcctggaa ccacagctgt cctgacccag acagtgaagc     1500

cactaaccct ggagctagtg gaggaaactg tgcaggctat ggaggtggag gaagggcgcg     1560

ggagcctgct gacatgtggg gacgtagaag aaaacccagg gccaatgctc ataggaaccc     1620

ccaccccgcg tgacactact cccagctcct ggctgacttc tagtcttctg gttgaagctg     1680

cgcctttaga tgacacgacc ctacccaccc ctgtttccag cggatgcccg ggcctggagc     1740

ccacagaatt cttccagtcc ctgggtgggg acggagaaag gaacgttcag attgagatgg     1800

cccatggcac caccacgctc gccttcaagt tccagcatgg agtgattgca gcagtggatt     1860

ctcgggcctc agctgggtcc tacattagtg ccttacgggt gaacaaggtg attgagatta     1920

acccttacct gcttggcacc atgtctggct gtgcagcaga ctgtcagtac tgggagcgcc     1980

tgctggccaa ggaatgcagg ctgtactatc tgcgaaatgg agaacgtatt tcagtgtcgg     2040

cagcctccaa gctgctgtcc aacatgatgt gccagtaccg gggcatgggc ctctctatgg     2100

gcagtatgat ctgtggctgg gataagaagg gtcctggact ctactacgtg gatgaacatg     2160

ggactcggct ctcaggaaat atgttctcca cgggtagtgg gaacacttat gcctacgggg     2220

tcatggacag tggctatcgg cctaatctta gccctgaaga ggcctatgac cttggccgca     2280

gggctattgc ttatgccact cacagagaca gctattctgg aggcgttgtc aatatgtacc     2340

acatgaagga agatggttgg gtgaaagtag aaagtacaga tgtcagtgac ctgctgcacc     2400

agtaccggga agccaatcaa taatgactgg atcc                                 2434


<210>  2
<211>  657
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(657)

<400>  2
atg ctg cgg gcg gga gca cca acc ggg gac tta ccc cgg gcg gga gaa         48
Met Leu Arg Ala Gly Ala Pro Thr Gly Asp Leu Pro Arg Ala Gly Glu           
1               5                   10                  15                

gtc cac acc ggg acc acc atc atg gca gtg gag ttt gac ggg ggc gtt         96
Val His Thr Gly Thr Thr Ile Met Ala Val Glu Phe Asp Gly Gly Val           
            20                  25                  30                    

gtg atg ggt tct gat tcc cga gtg tct gca ggc gag gcg gtg gtg aac        144
Val Met Gly Ser Asp Ser Arg Val Ser Ala Gly Glu Ala Val Val Asn           
        35                  40                  45                        

cga gtg ttt gac aag ctg tcc ccg ctg cac gag cgc atc tac tgt gca        192
Arg Val Phe Asp Lys Leu Ser Pro Leu His Glu Arg Ile Tyr Cys Ala           
    50                  55                  60                            

ctc tct ggt tca gct gct gat gcc caa gcc gtg gcc gac atg gcc gcc        240
Leu Ser Gly Ser Ala Ala Asp Ala Gln Ala Val Ala Asp Met Ala Ala           
65                  70                  75                  80            

tac cag ctg gag ctc cat ggg ata gaa ctg gag gaa cct cca ctt gtt        288
Tyr Gln Leu Glu Leu His Gly Ile Glu Leu Glu Glu Pro Pro Leu Val           
                85                  90                  95                

ttg gct gct gca aat gtg gtg aga aat atc agc tat aaa tat cga gag        336
Leu Ala Ala Ala Asn Val Val Arg Asn Ile Ser Tyr Lys Tyr Arg Glu           
            100                 105                 110                   

gac ttg tct gca cat ctc atg gta gct ggc tgg gac caa cgt gaa gga        384
Asp Leu Ser Ala His Leu Met Val Ala Gly Trp Asp Gln Arg Glu Gly           
        115                 120                 125                       

ggt cag gta tat gga acc ctg gga gga atg ctg act cga cag cct ttt        432
Gly Gln Val Tyr Gly Thr Leu Gly Gly Met Leu Thr Arg Gln Pro Phe           
    130                 135                 140                           

gcc att ggt ggc tcc ggc agc acc ttt atc tat ggt tat gtg gat gca        480
Ala Ile Gly Gly Ser Gly Ser Thr Phe Ile Tyr Gly Tyr Val Asp Ala           
145                 150                 155                 160           

gca tat aag cca ggc atg tct ccc gag gag tgc agg cgc ttc acc aca        528
Ala Tyr Lys Pro Gly Met Ser Pro Glu Glu Cys Arg Arg Phe Thr Thr           
                165                 170                 175               

gac gct att gct ctg gcc atg agc cgg gat ggc tca agc ggg ggt gtc        576
Asp Ala Ile Ala Leu Ala Met Ser Arg Asp Gly Ser Ser Gly Gly Val           
            180                 185                 190                   

atc tac ctg gtc act att aca gct gcc ggt gtg gac cat cga gtc atc        624
Ile Tyr Leu Val Thr Ile Thr Ala Ala Gly Val Asp His Arg Val Ile           
        195                 200                 205                       

ttg ggc aat gaa ctg cca aaa ttc tat gat gag                            657
Leu Gly Asn Glu Leu Pro Lys Phe Tyr Asp Glu                               
    210                 215                                               


<210>  3
<211>  219
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Leu Arg Ala Gly Ala Pro Thr Gly Asp Leu Pro Arg Ala Gly Glu 
1               5                   10                  15      


Val His Thr Gly Thr Thr Ile Met Ala Val Glu Phe Asp Gly Gly Val 
            20                  25                  30          


Val Met Gly Ser Asp Ser Arg Val Ser Ala Gly Glu Ala Val Val Asn 
        35                  40                  45              


Arg Val Phe Asp Lys Leu Ser Pro Leu His Glu Arg Ile Tyr Cys Ala 
    50                  55                  60                  


Leu Ser Gly Ser Ala Ala Asp Ala Gln Ala Val Ala Asp Met Ala Ala 
65                  70                  75                  80  


Tyr Gln Leu Glu Leu His Gly Ile Glu Leu Glu Glu Pro Pro Leu Val 
                85                  90                  95      


Leu Ala Ala Ala Asn Val Val Arg Asn Ile Ser Tyr Lys Tyr Arg Glu 
            100                 105                 110         


Asp Leu Ser Ala His Leu Met Val Ala Gly Trp Asp Gln Arg Glu Gly 
        115                 120                 125             


Gly Gln Val Tyr Gly Thr Leu Gly Gly Met Leu Thr Arg Gln Pro Phe 
    130                 135                 140                 


Ala Ile Gly Gly Ser Gly Ser Thr Phe Ile Tyr Gly Tyr Val Asp Ala 
145                 150                 155                 160 


Ala Tyr Lys Pro Gly Met Ser Pro Glu Glu Cys Arg Arg Phe Thr Thr 
                165                 170                 175     


Asp Ala Ile Ala Leu Ala Met Ser Arg Asp Gly Ser Ser Gly Gly Val 
            180                 185                 190         


Ile Tyr Leu Val Thr Ile Thr Ala Ala Gly Val Asp His Arg Val Ile 
        195                 200                 205             


Leu Gly Asn Glu Leu Pro Lys Phe Tyr Asp Glu 
    210                 215                 


<210>  4
<211>  819
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(819)

<400>  4
atg ctg aag cca gcc ctg gag ccc cga ggg ggc ttc tcc ttc gag aac         48
Met Leu Lys Pro Ala Leu Glu Pro Arg Gly Gly Phe Ser Phe Glu Asn           
1               5                   10                  15                

tgc caa aga aat gca tca ttg gaa cgc gtc ctc ccg ggg ctc aag gtc         96
Cys Gln Arg Asn Ala Ser Leu Glu Arg Val Leu Pro Gly Leu Lys Val           
            20                  25                  30                    

cct cac gca cgc aag acc ggg acc acc atc gcg ggc ctg gtg ttc caa        144
Pro His Ala Arg Lys Thr Gly Thr Thr Ile Ala Gly Leu Val Phe Gln           
        35                  40                  45                        

gac ggg gtc att ctg ggc gcc gat acg cga gcc act aac gat tcg gtc        192
Asp Gly Val Ile Leu Gly Ala Asp Thr Arg Ala Thr Asn Asp Ser Val           
    50                  55                  60                            

gtg gcg gac aag agc tgc gag aag atc cac ttc atc gcc ccc aaa atc        240
Val Ala Asp Lys Ser Cys Glu Lys Ile His Phe Ile Ala Pro Lys Ile           
65                  70                  75                  80            

tac tgc tgt ggg gct gga gta gcc gcg gac gcc gag atg acc aca cgg        288
Tyr Cys Cys Gly Ala Gly Val Ala Ala Asp Ala Glu Met Thr Thr Arg           
                85                  90                  95                

atg gtg gcg tcc aag atg gag cta cac gcg tta tct acg ggc cgc gag        336
Met Val Ala Ser Lys Met Glu Leu His Ala Leu Ser Thr Gly Arg Glu           
            100                 105                 110                   

ccc cgc gtg gcc acg gtc act cgc atc ctg cgc cag acg ctc ttc agg        384
Pro Arg Val Ala Thr Val Thr Arg Ile Leu Arg Gln Thr Leu Phe Arg           
        115                 120                 125                       

tac cag ggc cac gtg ggt gca tcg ctg atc gtg ggc ggc gta gac ctg        432
Tyr Gln Gly His Val Gly Ala Ser Leu Ile Val Gly Gly Val Asp Leu           
    130                 135                 140                           

act gga ccg cag ctc tac ggt gtg cat ccc cat ggc tcc tac agc cgt        480
Thr Gly Pro Gln Leu Tyr Gly Val His Pro His Gly Ser Tyr Ser Arg           
145                 150                 155                 160           

ctg ccc ttc aca gcc ctg ggc tct ggt cag gac gcg gcc ctg gcg gtg        528
Leu Pro Phe Thr Ala Leu Gly Ser Gly Gln Asp Ala Ala Leu Ala Val           
                165                 170                 175               

cta gaa gac cgg ttc cag ccg aac atg acg ctg gag gct gct cag ggg        576
Leu Glu Asp Arg Phe Gln Pro Asn Met Thr Leu Glu Ala Ala Gln Gly           
            180                 185                 190                   

ctg ctg gtg gaa gcc gtc acc gcc ggg atc ttg ggt gac ctg ggc tcc        624
Leu Leu Val Glu Ala Val Thr Ala Gly Ile Leu Gly Asp Leu Gly Ser           
        195                 200                 205                       

ggg ggc aat gtg gac gca tgt gtg atc aca aag act ggc gcc aag ctg        672
Gly Gly Asn Val Asp Ala Cys Val Ile Thr Lys Thr Gly Ala Lys Leu           
    210                 215                 220                           

ctg cgg aca ctg agc tca ccc aca gag ccc gtg aag agg tct ggc cgc        720
Leu Arg Thr Leu Ser Ser Pro Thr Glu Pro Val Lys Arg Ser Gly Arg           
225                 230                 235                 240           

tac cac ttt gtg cct gga acc aca gct gtc ctg acc cag aca gtg aag        768
Tyr His Phe Val Pro Gly Thr Thr Ala Val Leu Thr Gln Thr Val Lys           
                245                 250                 255               

cca cta acc ctg gag cta gtg gag gaa act gtg cag gct atg gag gtg        816
Pro Leu Thr Leu Glu Leu Val Glu Glu Thr Val Gln Ala Met Glu Val           
            260                 265                 270                   

gag                                                                    819
Glu                                                                       
                                                                          


<210>  5
<211>  273
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Leu Lys Pro Ala Leu Glu Pro Arg Gly Gly Phe Ser Phe Glu Asn 
1               5                   10                  15      


Cys Gln Arg Asn Ala Ser Leu Glu Arg Val Leu Pro Gly Leu Lys Val 
            20                  25                  30          


Pro His Ala Arg Lys Thr Gly Thr Thr Ile Ala Gly Leu Val Phe Gln 
        35                  40                  45              


Asp Gly Val Ile Leu Gly Ala Asp Thr Arg Ala Thr Asn Asp Ser Val 
    50                  55                  60                  


Val Ala Asp Lys Ser Cys Glu Lys Ile His Phe Ile Ala Pro Lys Ile 
65                  70                  75                  80  


Tyr Cys Cys Gly Ala Gly Val Ala Ala Asp Ala Glu Met Thr Thr Arg 
                85                  90                  95      


Met Val Ala Ser Lys Met Glu Leu His Ala Leu Ser Thr Gly Arg Glu 
            100                 105                 110         


Pro Arg Val Ala Thr Val Thr Arg Ile Leu Arg Gln Thr Leu Phe Arg 
        115                 120                 125             


Tyr Gln Gly His Val Gly Ala Ser Leu Ile Val Gly Gly Val Asp Leu 
    130                 135                 140                 


Thr Gly Pro Gln Leu Tyr Gly Val His Pro His Gly Ser Tyr Ser Arg 
145                 150                 155                 160 


Leu Pro Phe Thr Ala Leu Gly Ser Gly Gln Asp Ala Ala Leu Ala Val 
                165                 170                 175     


Leu Glu Asp Arg Phe Gln Pro Asn Met Thr Leu Glu Ala Ala Gln Gly 
            180                 185                 190         


Leu Leu Val Glu Ala Val Thr Ala Gly Ile Leu Gly Asp Leu Gly Ser 
        195                 200                 205             


Gly Gly Asn Val Asp Ala Cys Val Ile Thr Lys Thr Gly Ala Lys Leu 
    210                 215                 220                 


Leu Arg Thr Leu Ser Ser Pro Thr Glu Pro Val Lys Arg Ser Gly Arg 
225                 230                 235                 240 


Tyr His Phe Val Pro Gly Thr Thr Ala Val Leu Thr Gln Thr Val Lys 
                245                 250                 255     


Pro Leu Thr Leu Glu Leu Val Glu Glu Thr Val Gln Ala Met Glu Val 
            260                 265                 270         


Glu 
    


<210>  6
<211>  819
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(819)

<400>  6
atg ctc ata gga acc ccc acc ccg cgt gac act act ccc agc tcc tgg         48
Met Leu Ile Gly Thr Pro Thr Pro Arg Asp Thr Thr Pro Ser Ser Trp           
1               5                   10                  15                

ctg act tct agt ctt ctg gtt gaa gct gcg cct tta gat gac acg acc         96
Leu Thr Ser Ser Leu Leu Val Glu Ala Ala Pro Leu Asp Asp Thr Thr           
            20                  25                  30                    

cta ccc acc cct gtt tcc agc gga tgc ccg ggc ctg gag ccc aca gaa        144
Leu Pro Thr Pro Val Ser Ser Gly Cys Pro Gly Leu Glu Pro Thr Glu           
        35                  40                  45                        

ttc ttc cag tcc ctg ggt ggg gac gga gaa agg aac gtt cag att gag        192
Phe Phe Gln Ser Leu Gly Gly Asp Gly Glu Arg Asn Val Gln Ile Glu           
    50                  55                  60                            

atg gcc cat ggc acc acc acg ctc gcc ttc aag ttc cag cat gga gtg        240
Met Ala His Gly Thr Thr Thr Leu Ala Phe Lys Phe Gln His Gly Val           
65                  70                  75                  80            

att gca gca gtg gat tct cgg gcc tca gct ggg tcc tac att agt gcc        288
Ile Ala Ala Val Asp Ser Arg Ala Ser Ala Gly Ser Tyr Ile Ser Ala           
                85                  90                  95                

tta cgg gtg aac aag gtg att gag att aac cct tac ctg ctt ggc acc        336
Leu Arg Val Asn Lys Val Ile Glu Ile Asn Pro Tyr Leu Leu Gly Thr           
            100                 105                 110                   

atg tct ggc tgt gca gca gac tgt cag tac tgg gag cgc ctg ctg gcc        384
Met Ser Gly Cys Ala Ala Asp Cys Gln Tyr Trp Glu Arg Leu Leu Ala           
        115                 120                 125                       

aag gaa tgc agg ctg tac tat ctg cga aat gga gaa cgt att tca gtg        432
Lys Glu Cys Arg Leu Tyr Tyr Leu Arg Asn Gly Glu Arg Ile Ser Val           
    130                 135                 140                           

tcg gca gcc tcc aag ctg ctg tcc aac atg atg tgc cag tac cgg ggc        480
Ser Ala Ala Ser Lys Leu Leu Ser Asn Met Met Cys Gln Tyr Arg Gly           
145                 150                 155                 160           

atg ggc ctc tct atg ggc agt atg atc tgt ggc tgg gat aag aag ggt        528
Met Gly Leu Ser Met Gly Ser Met Ile Cys Gly Trp Asp Lys Lys Gly           
                165                 170                 175               

cct gga ctc tac tac gtg gat gaa cat ggg act cgg ctc tca gga aat        576
Pro Gly Leu Tyr Tyr Val Asp Glu His Gly Thr Arg Leu Ser Gly Asn           
            180                 185                 190                   

atg ttc tcc acg ggt agt ggg aac act tat gcc tac ggg gtc atg gac        624
Met Phe Ser Thr Gly Ser Gly Asn Thr Tyr Ala Tyr Gly Val Met Asp           
        195                 200                 205                       

agt ggc tat cgg cct aat ctt agc cct gaa gag gcc tat gac ctt ggc        672
Ser Gly Tyr Arg Pro Asn Leu Ser Pro Glu Glu Ala Tyr Asp Leu Gly           
    210                 215                 220                           

cgc agg gct att gct tat gcc act cac aga gac agc tat tct gga ggc        720
Arg Arg Ala Ile Ala Tyr Ala Thr His Arg Asp Ser Tyr Ser Gly Gly           
225                 230                 235                 240           

gtt gtc aat atg tac cac atg aag gaa gat ggt tgg gtg aaa gta gaa        768
Val Val Asn Met Tyr His Met Lys Glu Asp Gly Trp Val Lys Val Glu           
                245                 250                 255               

agt aca gat gtc agt gac ctg ctg cac cag tac cgg gaa gcc aat caa        816
Ser Thr Asp Val Ser Asp Leu Leu His Gln Tyr Arg Glu Ala Asn Gln           
            260                 265                 270                   

taa                                                                    819


<210>  7
<211>  272
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Leu Ile Gly Thr Pro Thr Pro Arg Asp Thr Thr Pro Ser Ser Trp 
1               5                   10                  15      


Leu Thr Ser Ser Leu Leu Val Glu Ala Ala Pro Leu Asp Asp Thr Thr 
            20                  25                  30          


Leu Pro Thr Pro Val Ser Ser Gly Cys Pro Gly Leu Glu Pro Thr Glu 
        35                  40                  45              


Phe Phe Gln Ser Leu Gly Gly Asp Gly Glu Arg Asn Val Gln Ile Glu 
    50                  55                  60                  


Met Ala His Gly Thr Thr Thr Leu Ala Phe Lys Phe Gln His Gly Val 
65                  70                  75                  80  


Ile Ala Ala Val Asp Ser Arg Ala Ser Ala Gly Ser Tyr Ile Ser Ala 
                85                  90                  95      


Leu Arg Val Asn Lys Val Ile Glu Ile Asn Pro Tyr Leu Leu Gly Thr 
            100                 105                 110         


Met Ser Gly Cys Ala Ala Asp Cys Gln Tyr Trp Glu Arg Leu Leu Ala 
        115                 120                 125             


Lys Glu Cys Arg Leu Tyr Tyr Leu Arg Asn Gly Glu Arg Ile Ser Val 
    130                 135                 140                 


Ser Ala Ala Ser Lys Leu Leu Ser Asn Met Met Cys Gln Tyr Arg Gly 
145                 150                 155                 160 


Met Gly Leu Ser Met Gly Ser Met Ile Cys Gly Trp Asp Lys Lys Gly 
                165                 170                 175     


Pro Gly Leu Tyr Tyr Val Asp Glu His Gly Thr Arg Leu Ser Gly Asn 
            180                 185                 190         


Met Phe Ser Thr Gly Ser Gly Asn Thr Tyr Ala Tyr Gly Val Met Asp 
        195                 200                 205             


Ser Gly Tyr Arg Pro Asn Leu Ser Pro Glu Glu Ala Tyr Asp Leu Gly 
    210                 215                 220                 


Arg Arg Ala Ile Ala Tyr Ala Thr His Arg Asp Ser Tyr Ser Gly Gly 
225                 230                 235                 240 


Val Val Asn Met Tyr His Met Lys Glu Asp Gly Trp Val Lys Val Glu 
                245                 250                 255     


Ser Thr Asp Val Ser Asp Leu Leu His Gln Tyr Arg Glu Ala Asn Gln 
            260                 265                 270         


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A connector sequence


<220>
<221>  CDS
<222>  (1)..(54)

<400>  8
gaa ggg cgc ggg agc ctg ctg aca tgt ggg gac gta gaa gaa aac cca         48
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro           
1               5                   10                  15                

ggg cca                                                                 54
Gly Pro                                                                   
                                                                          


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  10
<211>  2420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Viral transduction construct

<400>  10
tcgagacgcg ggcggccacc atggccgagc cccgctctgc tgaggccacc atgctgcggg       60

caggagcacc taccgccggc tcgttccgga cggaagaagt ccacaccggg acaaccatca      120

tggcagtgga gtttgacggg ggtgtcgtgg tgggctctga ttcccgggtg tcagcgggaa      180

cagcagtggt gaaccgcgtg ttcgacaagc tctcccctct gcaccagcac atcttctgtg      240

ccctctcagg ttccgctgct gatgcccaag ccatagctga catggccgcc taccagctgg      300

agctacacgg gttggagctg gaggagccgc ccctcgttct ggctgctgca aacgtggtga      360

agaacatctc ctacaagtac cgtgaggact tgttagcgca tctcatagta gctggctggg      420

accaatgtga ggggggacag gtatatggaa ccatgggagg gatgctaatt cgacagccct      480

caggcatgac ccctgaggag tgccggcgtt tcaccacaga tgccatcact ctggccatga      540

accgagatgg ctctagtggg ggtgtcatct acctggtcac catcacagct gctggtgtgg      600

accatcgagt catcctggga gatgagctgc caaaattcta cgatgaggaa gggcgcggga      660

gcctgctgac atgtggggac gtagaagaaa acccagggcc aatgctgaag caggcagtgg      720

aacccacagg aggcttctct ttcgagaact gccagaggaa tgcgtccttg gaacacgtcc      780

ttccgggact tcgggttcct catgcacgca agaccgggac taccatcgcg gggcttgtgt      840

tccgagatgg agtcatcctg ggagcggaca cgcgggccac taacgattcg gttgtggcgg      900

acaaaagctg cgagaagatc cacttcatcg cccctaaaat ctactgctgt ggggctggag      960

tagctgcgga cactgagatg actacgcgga tggcagcttc caagatggaa ctacatgcgc     1020

tgtccaccgg ccgtgagcct cgggtggcca cggtcacccg tatcctgcgc cagacgcttt     1080

tccggtacca aggccacgtg ggggcatcac tggtcgtggg cggggttgat ttgaacggac     1140

ctcagctcta cgaggtgcac ccacatggtt cctacagccg tctgcccttt actgcccttg     1200

gctctggtca gggtgcagcc gtggcactgt tggaagaccg gttccagcca aacatgacgc     1260

tggaggctgc gcaagagctg ttggtggaag ccatcacagc agggatactg agtgacctgg     1320

gctctggggg caatgtggat gcctgtgtga tcactgcagg gggtgccaag ctgcagagag     1380

cattgagcac ccccaccgag cctgtgcaga gagctggccg ttaccgattt gctcctggaa     1440

ccacacctgt cctgacccgg gaagtgagac ccctgaccct ggaacttctt gaggaaactg     1500

tgcaggccat ggaggtggaa gaagggcgcg ggagcctgct gacatgtggg gacgtagaag     1560

aaaacccagg gccaatggcg ttactggatc tgtgcggtgc cgctcggggg cagcggcccg     1620

agtgggctgc cctggatgcg ggaagcgggg gtcgctcgga cccgggacac tacagtttct     1680

ccgcgcaagc tccggagctc gcacttcccc ggggaatgca gcccaccgca ttcctgaggt     1740

cctttggtgg tgaccaggaa aggaatgttc aaattgagat ggcccacggc acaaccacac     1800

tcgccttcaa gttccagcat ggcgtcatcg tggctgtgga ctccagggcc actgcaggga     1860

gttacattag ctccttaagg atgaacaaag tgatcgagat taacccttac ctgcttggca     1920

ccatgtctgg ttgtgcagcc gactgccagt actgggagag gctgttggcc aaggagtgca     1980

ggttgtatta tcttcggaat ggggaacgca tctccgtgtc tgcagcatcc aagctgcttt     2040

ccaacatgat gctgcagtac cgggggatgg gcctctccat gggcagcatg atctgtggct     2100

gggacaagaa gggaccagga ctttactacg tagatgacaa tgggactcgg ctctcgggac     2160

agatgttttc cactggcagc gggaacacct atgcctatgg ggtgatggac agtggttacc     2220

ggcaggacct cagtcctgaa gaggcctacg accttggccg cagagctatt gcttatgcta     2280

cccacagaga caactattct ggaggagtcg tcaacatgta ccacatgaag gaagacggtt     2340

gggtgaaagt ggagagttcc gatgtcagtg acctgctgta caagtacgga gaggccgctc     2400

tgtgatggct gactggatcc                                                 2420


<210>  11
<211>  597
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (1)..(597)

<400>  11
atg ctg cgg gca gga gca cct acc gcc ggc tcg ttc cgg acg gaa gaa         48
Met Leu Arg Ala Gly Ala Pro Thr Ala Gly Ser Phe Arg Thr Glu Glu           
1               5                   10                  15                

gtc cac acc ggg aca acc atc atg gca gtg gag ttt gac ggg ggt gtc         96
Val His Thr Gly Thr Thr Ile Met Ala Val Glu Phe Asp Gly Gly Val           
            20                  25                  30                    

gtg gtg ggc tct gat tcc cgg gtg tca gcg gga aca gca gtg gtg aac        144
Val Val Gly Ser Asp Ser Arg Val Ser Ala Gly Thr Ala Val Val Asn           
        35                  40                  45                        

cgc gtg ttc gac aag ctc tcc cct ctg cac cag cac atc ttc tgt gcc        192
Arg Val Phe Asp Lys Leu Ser Pro Leu His Gln His Ile Phe Cys Ala           
    50                  55                  60                            

ctc tca ggt tcc gct gct gat gcc caa gcc ata gct gac atg gcc gcc        240
Leu Ser Gly Ser Ala Ala Asp Ala Gln Ala Ile Ala Asp Met Ala Ala           
65                  70                  75                  80            

tac cag ctg gag cta cac ggg ttg gag ctg gag gag ccg ccc ctc gtt        288
Tyr Gln Leu Glu Leu His Gly Leu Glu Leu Glu Glu Pro Pro Leu Val           
                85                  90                  95                

ctg gct gct gca aac gtg gtg aag aac atc tcc tac aag tac cgt gag        336
Leu Ala Ala Ala Asn Val Val Lys Asn Ile Ser Tyr Lys Tyr Arg Glu           
            100                 105                 110                   

gac ttg tta gcg cat ctc ata gta gct ggc tgg gac caa tgt gag ggg        384
Asp Leu Leu Ala His Leu Ile Val Ala Gly Trp Asp Gln Cys Glu Gly           
        115                 120                 125                       

gga cag gta tat gga acc atg gga ggg atg cta att cga cag ccc tca        432
Gly Gln Val Tyr Gly Thr Met Gly Gly Met Leu Ile Arg Gln Pro Ser           
    130                 135                 140                           

ggc atg acc cct gag gag tgc cgg cgt ttc acc aca gat gcc atc act        480
Gly Met Thr Pro Glu Glu Cys Arg Arg Phe Thr Thr Asp Ala Ile Thr           
145                 150                 155                 160           

ctg gcc atg aac cga gat ggc tct agt ggg ggt gtc atc tac ctg gtc        528
Leu Ala Met Asn Arg Asp Gly Ser Ser Gly Gly Val Ile Tyr Leu Val           
                165                 170                 175               

acc atc aca gct gct ggt gtg gac cat cga gtc atc ctg gga gat gag        576
Thr Ile Thr Ala Ala Gly Val Asp His Arg Val Ile Leu Gly Asp Glu           
            180                 185                 190                   

ctg cca aaa ttc tac gat gag                                            597
Leu Pro Lys Phe Tyr Asp Glu                                               
        195                                                               


<210>  12
<211>  199
<212>  PRT
<213>  Mus musculus

<400>  12

Met Leu Arg Ala Gly Ala Pro Thr Ala Gly Ser Phe Arg Thr Glu Glu 
1               5                   10                  15      


Val His Thr Gly Thr Thr Ile Met Ala Val Glu Phe Asp Gly Gly Val 
            20                  25                  30          


Val Val Gly Ser Asp Ser Arg Val Ser Ala Gly Thr Ala Val Val Asn 
        35                  40                  45              


Arg Val Phe Asp Lys Leu Ser Pro Leu His Gln His Ile Phe Cys Ala 
    50                  55                  60                  


Leu Ser Gly Ser Ala Ala Asp Ala Gln Ala Ile Ala Asp Met Ala Ala 
65                  70                  75                  80  


Tyr Gln Leu Glu Leu His Gly Leu Glu Leu Glu Glu Pro Pro Leu Val 
                85                  90                  95      


Leu Ala Ala Ala Asn Val Val Lys Asn Ile Ser Tyr Lys Tyr Arg Glu 
            100                 105                 110         


Asp Leu Leu Ala His Leu Ile Val Ala Gly Trp Asp Gln Cys Glu Gly 
        115                 120                 125             


Gly Gln Val Tyr Gly Thr Met Gly Gly Met Leu Ile Arg Gln Pro Ser 
    130                 135                 140                 


Gly Met Thr Pro Glu Glu Cys Arg Arg Phe Thr Thr Asp Ala Ile Thr 
145                 150                 155                 160 


Leu Ala Met Asn Arg Asp Gly Ser Ser Gly Gly Val Ile Tyr Leu Val 
                165                 170                 175     


Thr Ile Thr Ala Ala Gly Val Asp His Arg Val Ile Leu Gly Asp Glu 
            180                 185                 190         


Leu Pro Lys Phe Tyr Asp Glu 
        195                 


<210>  13
<211>  819
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (1)..(819)

<400>  13
atg ctg aag cag gca gtg gaa ccc aca gga ggc ttc tct ttc gag aac         48
Met Leu Lys Gln Ala Val Glu Pro Thr Gly Gly Phe Ser Phe Glu Asn           
1               5                   10                  15                

tgc cag agg aat gcg tcc ttg gaa cac gtc ctt ccg gga ctt cgg gtt         96
Cys Gln Arg Asn Ala Ser Leu Glu His Val Leu Pro Gly Leu Arg Val           
            20                  25                  30                    

cct cat gca cgc aag acc ggg act acc atc gcg ggg ctt gtg ttc cga        144
Pro His Ala Arg Lys Thr Gly Thr Thr Ile Ala Gly Leu Val Phe Arg           
        35                  40                  45                        

gat gga gtc atc ctg gga gcg gac acg cgg gcc act aac gat tcg gtt        192
Asp Gly Val Ile Leu Gly Ala Asp Thr Arg Ala Thr Asn Asp Ser Val           
    50                  55                  60                            

gtg gcg gac aaa agc tgc gag aag atc cac ttc atc gcc cct aaa atc        240
Val Ala Asp Lys Ser Cys Glu Lys Ile His Phe Ile Ala Pro Lys Ile           
65                  70                  75                  80            

tac tgc tgt ggg gct gga gta gct gcg gac act gag atg act acg cgg        288
Tyr Cys Cys Gly Ala Gly Val Ala Ala Asp Thr Glu Met Thr Thr Arg           
                85                  90                  95                

atg gca gct tcc aag atg gaa cta cat gcg ctg tcc acc ggc cgt gag        336
Met Ala Ala Ser Lys Met Glu Leu His Ala Leu Ser Thr Gly Arg Glu           
            100                 105                 110                   

cct cgg gtg gcc acg gtc acc cgt atc ctg cgc cag acg ctt ttc cgg        384
Pro Arg Val Ala Thr Val Thr Arg Ile Leu Arg Gln Thr Leu Phe Arg           
        115                 120                 125                       

tac caa ggc cac gtg ggg gca tca ctg gtc gtg ggc ggg gtt gat ttg        432
Tyr Gln Gly His Val Gly Ala Ser Leu Val Val Gly Gly Val Asp Leu           
    130                 135                 140                           

aac gga cct cag ctc tac gag gtg cac cca cat ggt tcc tac agc cgt        480
Asn Gly Pro Gln Leu Tyr Glu Val His Pro His Gly Ser Tyr Ser Arg           
145                 150                 155                 160           

ctg ccc ttt act gcc ctt ggc tct ggt cag ggt gca gcc gtg gca ctg        528
Leu Pro Phe Thr Ala Leu Gly Ser Gly Gln Gly Ala Ala Val Ala Leu           
                165                 170                 175               

ttg gaa gac cgg ttc cag cca aac atg acg ctg gag gct gcg caa gag        576
Leu Glu Asp Arg Phe Gln Pro Asn Met Thr Leu Glu Ala Ala Gln Glu           
            180                 185                 190                   

ctg ttg gtg gaa gcc atc aca gca ggg ata ctg agt gac ctg ggc tct        624
Leu Leu Val Glu Ala Ile Thr Ala Gly Ile Leu Ser Asp Leu Gly Ser           
        195                 200                 205                       

ggg ggc aat gtg gat gcc tgt gtg atc act gca ggg ggt gcc aag ctg        672
Gly Gly Asn Val Asp Ala Cys Val Ile Thr Ala Gly Gly Ala Lys Leu           
    210                 215                 220                           

cag aga gca ttg agc acc ccc acc gag cct gtg cag aga gct ggc cgt        720
Gln Arg Ala Leu Ser Thr Pro Thr Glu Pro Val Gln Arg Ala Gly Arg           
225                 230                 235                 240           

tac cga ttt gct cct gga acc aca cct gtc ctg acc cgg gaa gtg aga        768
Tyr Arg Phe Ala Pro Gly Thr Thr Pro Val Leu Thr Arg Glu Val Arg           
                245                 250                 255               

ccc ctg acc ctg gaa ctt ctt gag gaa act gtg cag gcc atg gag gtg        816
Pro Leu Thr Leu Glu Leu Leu Glu Glu Thr Val Gln Ala Met Glu Val           
            260                 265                 270                   

gaa                                                                    819
Glu                                                                       
                                                                          


<210>  14
<211>  273
<212>  PRT
<213>  Mus musculus

<400>  14

Met Leu Lys Gln Ala Val Glu Pro Thr Gly Gly Phe Ser Phe Glu Asn 
1               5                   10                  15      


Cys Gln Arg Asn Ala Ser Leu Glu His Val Leu Pro Gly Leu Arg Val 
            20                  25                  30          


Pro His Ala Arg Lys Thr Gly Thr Thr Ile Ala Gly Leu Val Phe Arg 
        35                  40                  45              


Asp Gly Val Ile Leu Gly Ala Asp Thr Arg Ala Thr Asn Asp Ser Val 
    50                  55                  60                  


Val Ala Asp Lys Ser Cys Glu Lys Ile His Phe Ile Ala Pro Lys Ile 
65                  70                  75                  80  


Tyr Cys Cys Gly Ala Gly Val Ala Ala Asp Thr Glu Met Thr Thr Arg 
                85                  90                  95      


Met Ala Ala Ser Lys Met Glu Leu His Ala Leu Ser Thr Gly Arg Glu 
            100                 105                 110         


Pro Arg Val Ala Thr Val Thr Arg Ile Leu Arg Gln Thr Leu Phe Arg 
        115                 120                 125             


Tyr Gln Gly His Val Gly Ala Ser Leu Val Val Gly Gly Val Asp Leu 
    130                 135                 140                 


Asn Gly Pro Gln Leu Tyr Glu Val His Pro His Gly Ser Tyr Ser Arg 
145                 150                 155                 160 


Leu Pro Phe Thr Ala Leu Gly Ser Gly Gln Gly Ala Ala Val Ala Leu 
                165                 170                 175     


Leu Glu Asp Arg Phe Gln Pro Asn Met Thr Leu Glu Ala Ala Gln Glu 
            180                 185                 190         


Leu Leu Val Glu Ala Ile Thr Ala Gly Ile Leu Ser Asp Leu Gly Ser 
        195                 200                 205             


Gly Gly Asn Val Asp Ala Cys Val Ile Thr Ala Gly Gly Ala Lys Leu 
    210                 215                 220                 


Gln Arg Ala Leu Ser Thr Pro Thr Glu Pro Val Gln Arg Ala Gly Arg 
225                 230                 235                 240 


Tyr Arg Phe Ala Pro Gly Thr Thr Pro Val Leu Thr Arg Glu Val Arg 
                245                 250                 255     


Pro Leu Thr Leu Glu Leu Leu Glu Glu Thr Val Gln Ala Met Glu Val 
            260                 265                 270         


Glu 
    


<210>  15
<211>  835
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (1)..(834)

<400>  15
atg gcg tta ctg gat ctg tgc ggt gcc gct cgg ggg cag cgg ccc gag         48
Met Ala Leu Leu Asp Leu Cys Gly Ala Ala Arg Gly Gln Arg Pro Glu           
1               5                   10                  15                

tgg gct gcc ctg gat gcg gga agc ggg ggt cgc tcg gac ccg gga cac         96
Trp Ala Ala Leu Asp Ala Gly Ser Gly Gly Arg Ser Asp Pro Gly His           
            20                  25                  30                    

tac agt ttc tcc gcg caa gct ccg gag ctc gca ctt ccc cgg gga atg        144
Tyr Ser Phe Ser Ala Gln Ala Pro Glu Leu Ala Leu Pro Arg Gly Met           
        35                  40                  45                        

cag ccc acc gca ttc ctg agg tcc ttt ggt ggt gac cag gaa agg aat        192
Gln Pro Thr Ala Phe Leu Arg Ser Phe Gly Gly Asp Gln Glu Arg Asn           
    50                  55                  60                            

gtt caa att gag atg gcc cac ggc aca acc aca ctc gcc ttc aag ttc        240
Val Gln Ile Glu Met Ala His Gly Thr Thr Thr Leu Ala Phe Lys Phe           
65                  70                  75                  80            

cag cat ggc gtc atc gtg gct gtg gac tcc agg gcc act gca ggg agt        288
Gln His Gly Val Ile Val Ala Val Asp Ser Arg Ala Thr Ala Gly Ser           
                85                  90                  95                

tac att agc tcc tta agg atg aac aaa gtg atc gag att aac cct tac        336
Tyr Ile Ser Ser Leu Arg Met Asn Lys Val Ile Glu Ile Asn Pro Tyr           
            100                 105                 110                   

ctg ctt ggc acc atg tct ggt tgt gca gcc gac tgc cag tac tgg gag        384
Leu Leu Gly Thr Met Ser Gly Cys Ala Ala Asp Cys Gln Tyr Trp Glu           
        115                 120                 125                       

agg ctg ttg gcc aag gag tgc agg ttg tat tat ctt cgg aat ggg gaa        432
Arg Leu Leu Ala Lys Glu Cys Arg Leu Tyr Tyr Leu Arg Asn Gly Glu           
    130                 135                 140                           

cgc atc tcc gtg tct gca gca tcc aag ctg ctt tcc aac atg atg ctg        480
Arg Ile Ser Val Ser Ala Ala Ser Lys Leu Leu Ser Asn Met Met Leu           
145                 150                 155                 160           

cag tac cgg ggg atg ggc ctc tcc atg ggc agc atg atc tgt ggc tgg        528
Gln Tyr Arg Gly Met Gly Leu Ser Met Gly Ser Met Ile Cys Gly Trp           
                165                 170                 175               

gac aag aag gga cca gga ctt tac tac gta gat gac aat ggg act cgg        576
Asp Lys Lys Gly Pro Gly Leu Tyr Tyr Val Asp Asp Asn Gly Thr Arg           
            180                 185                 190                   

ctc tcg gga cag atg ttt tcc act ggc agc ggg aac acc tat gcc tat        624
Leu Ser Gly Gln Met Phe Ser Thr Gly Ser Gly Asn Thr Tyr Ala Tyr           
        195                 200                 205                       

ggg gtg atg gac agt ggt tac cgg cag gac ctc agt cct gaa gag gcc        672
Gly Val Met Asp Ser Gly Tyr Arg Gln Asp Leu Ser Pro Glu Glu Ala           
    210                 215                 220                           

tac gac ctt ggc cgc aga gct att gct tat gct acc cac aga gac aac        720
Tyr Asp Leu Gly Arg Arg Ala Ile Ala Tyr Ala Thr His Arg Asp Asn           
225                 230                 235                 240           

tat tct gga gga gtc gtc aac atg tac cac atg aag gaa gac ggt tgg        768
Tyr Ser Gly Gly Val Val Asn Met Tyr His Met Lys Glu Asp Gly Trp           
                245                 250                 255               

gtg aaa gtg gag agt tcc gat gtc agt gac ctg ctg tac aag tac gga        816
Val Lys Val Glu Ser Ser Asp Val Ser Asp Leu Leu Tyr Lys Tyr Gly           
            260                 265                 270                   

gag gcc gct ctg tga tgg c                                              835
Glu Ala Ala Leu     Trp                                                   
        275                                                               


<210>  16
<211>  276
<212>  PRT
<213>  Mus musculus

<400>  16

Met Ala Leu Leu Asp Leu Cys Gly Ala Ala Arg Gly Gln Arg Pro Glu 
1               5                   10                  15      


Trp Ala Ala Leu Asp Ala Gly Ser Gly Gly Arg Ser Asp Pro Gly His 
            20                  25                  30          


Tyr Ser Phe Ser Ala Gln Ala Pro Glu Leu Ala Leu Pro Arg Gly Met 
        35                  40                  45              


Gln Pro Thr Ala Phe Leu Arg Ser Phe Gly Gly Asp Gln Glu Arg Asn 
    50                  55                  60                  


Val Gln Ile Glu Met Ala His Gly Thr Thr Thr Leu Ala Phe Lys Phe 
65                  70                  75                  80  


Gln His Gly Val Ile Val Ala Val Asp Ser Arg Ala Thr Ala Gly Ser 
                85                  90                  95      


Tyr Ile Ser Ser Leu Arg Met Asn Lys Val Ile Glu Ile Asn Pro Tyr 
            100                 105                 110         


Leu Leu Gly Thr Met Ser Gly Cys Ala Ala Asp Cys Gln Tyr Trp Glu 
        115                 120                 125             


Arg Leu Leu Ala Lys Glu Cys Arg Leu Tyr Tyr Leu Arg Asn Gly Glu 
    130                 135                 140                 


Arg Ile Ser Val Ser Ala Ala Ser Lys Leu Leu Ser Asn Met Met Leu 
145                 150                 155                 160 


Gln Tyr Arg Gly Met Gly Leu Ser Met Gly Ser Met Ile Cys Gly Trp 
                165                 170                 175     


Asp Lys Lys Gly Pro Gly Leu Tyr Tyr Val Asp Asp Asn Gly Thr Arg 
            180                 185                 190         


Leu Ser Gly Gln Met Phe Ser Thr Gly Ser Gly Asn Thr Tyr Ala Tyr 
        195                 200                 205             


Gly Val Met Asp Ser Gly Tyr Arg Gln Asp Leu Ser Pro Glu Glu Ala 
    210                 215                 220                 


Tyr Asp Leu Gly Arg Arg Ala Ile Ala Tyr Ala Thr His Arg Asp Asn 
225                 230                 235                 240 


Tyr Ser Gly Gly Val Val Asn Met Tyr His Met Lys Glu Asp Gly Trp 
                245                 250                 255     


Val Lys Val Glu Ser Ser Asp Val Ser Asp Leu Leu Tyr Lys Tyr Gly 
            260                 265                 270         


Glu Ala Ala Leu 
        275     


<210>  17
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A connector sequence

<400>  17

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  18
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A connector sequence

<400>  18

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  19
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A connector sequence

<400>  19

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


