                         SEQUENCE LISTING

<110>  School of Life Sciences, Peking University
 
<120>  COMPOSITIONS AND METHODS FOR IMPROVING HEALTH THROUGH MODULATING 
       CALHM2

<130>  20314-0002WO00

<160>  22    

<170>  PatentIn version 3.3

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  1
tcctggtgtt cctgaccaag tg                                              22


<210>  2
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  2
aagtcacctg atgctgaaat gagc                                            24


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  3
gcatcgcatt gtctgagtag gtg                                             23


<210>  4
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Forward primer sequence for encoding the sgRNA

<400>  4
caccgtccgg ccccgaggag gcc                                             23


<210>  5
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Reverse primer sequence for encoding the sgRNA

<400>  5
aaactggcct cctcggggcc ggac                                            24


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  6
tcacctggtc tgtcatctcc                                                 20


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
tgagtggcga gcagtaatg                                                  19


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
tgagtttgtg gacccctcct                                                 20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
gctgagcggt gagcagtaat                                                 20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  10
gctatttgcg ctgcatctct                                                 20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  11
ttgagaaagg caacctgcgt                                                 20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  12
ctgcaaggaa gatgagctgg t                                               21


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  13
tcagacagcg ggctaggaag                                                 20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  14
atcaagaagg tggtgaagca                                                 20


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  15
aaggtggaag aatgggagtt g                                               21


<210>  16
<211>  322
<212>  PRT
<213>  rat

<400>  16

Met Ala Ala Leu Ile Ala Glu Asn Phe Arg Phe Leu Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Gln Glu Leu Phe Ser Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Ala Arg Asn Tyr Leu Tyr Gly Leu Thr Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Ala Leu Phe Leu Ile Gly Val Ile Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Leu Val Ala Glu Cys Gln Tyr Arg Arg Ala Lys Asn Cys Ser Ala 
                85                  90                  95      


Ala Pro Thr Phe Leu Leu Leu Ser Ser Ile Leu Gly Arg Ala Ala Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Leu Ser Glu Phe Val Asp Pro Ser Ser Leu Thr Ala Gly 
    130                 135                 140                 


Asp Glu Gly Phe Pro Pro Asp His Ala Thr Glu Ile Leu Ala Arg Phe 
145                 150                 155                 160 


Pro Cys Gly Glu Gly Pro Ala Asn Leu Ser Gly Phe Arg Glu Glu Val 
                165                 170                 175     


Ser Arg Arg Leu Lys Tyr Glu Ser Gln Leu Phe Gly Trp Leu Leu Ile 
            180                 185                 190         


Gly Val Val Ala Ile Leu Val Phe Leu Thr Lys Cys Phe Lys His Tyr 
        195                 200                 205             


Cys Ser Pro Leu Ser Tyr Arg Gln Glu Ala Tyr Trp Ala Gln Tyr Arg 
    210                 215                 220                 


Thr Asn Glu Asp Gln Leu Phe Gln Arg Thr Ala Glu Val His Ser Arg 
225                 230                 235                 240 


Val Leu Ala Ala Asn Asn Val Arg Arg Phe Phe Gly Phe Val Ala Leu 
                245                 250                 255     


Asn Lys Asp Asp Glu Glu Leu Val Thr Lys Phe Pro Val Glu Gly Thr 
            260                 265                 270         


Gln Pro Arg Pro Gln Trp Asn Ala Ile Thr Gly Val Tyr Leu Tyr Arg 
        275                 280                 285             


Glu Asn Gln Gly Leu Pro Leu Tyr Ser Arg Leu His Lys Trp Ala Gln 
    290                 295                 300                 


Gly Leu Thr Gly Asn Gly Thr Ala Pro Asp Asn Val Glu Met Ala Leu 
305                 310                 315                 320 


Leu Thr 
        


<210>  17
<211>  322
<212>  PRT
<213>  mouse

<400>  17

Met Ala Ala Leu Ile Ala Glu Asn Phe Arg Phe Leu Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Gln Glu Leu Phe Ser Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Ala Arg Asn Tyr Leu Tyr Gly Leu Thr Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Ala Leu Phe Leu Ile Gly Val Ile Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Leu Val Ala Glu Cys Gln Tyr Arg Arg Ala Lys Asn Cys Ser Ala 
                85                  90                  95      


Ala Pro Asn Phe Leu Leu Leu Ser Ser Ile Leu Gly Arg Ala Ala Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Leu Ser Glu Phe Val Asp Pro Ser Ser Leu Thr Ala Gly 
    130                 135                 140                 


Asp Lys Gly Phe Pro Pro Ala His Ala Thr Glu Val Leu Ala Arg Phe 
145                 150                 155                 160 


Pro Cys Gly Glu Gly Pro Ala Asn Leu Ser Ser Phe Arg Glu Glu Val 
                165                 170                 175     


Ser Arg Arg Leu Lys Tyr Glu Ser Gln Leu Phe Gly Trp Leu Leu Ile 
            180                 185                 190         


Gly Val Val Ala Ile Leu Val Phe Leu Thr Lys Cys Leu Lys His Tyr 
        195                 200                 205             


Cys Ser Pro Leu Ser Tyr Arg Gln Glu Ala Tyr Trp Ala Gln Tyr Arg 
    210                 215                 220                 


Thr Asn Glu Asp Gln Leu Phe Gln Arg Thr Ala Glu Val His Ser Arg 
225                 230                 235                 240 


Val Leu Ala Ala Asn Asn Val Arg Arg Phe Phe Gly Phe Val Ala Leu 
                245                 250                 255     


Asn Lys Asp Asp Glu Glu Leu Val Ala Lys Phe Pro Val Glu Gly Thr 
            260                 265                 270         


Gln Pro Arg Pro Gln Trp Asn Ala Ile Thr Gly Val Tyr Leu Tyr Arg 
        275                 280                 285             


Glu Asn Gln Gly Leu Pro Leu Tyr Ser Arg Leu His Lys Trp Ala Gln 
    290                 295                 300                 


Gly Leu Thr Gly Asn Gly Thr Ala Pro Asp Asn Val Glu Met Ala Leu 
305                 310                 315                 320 


Leu Thr 
        


<210>  18
<211>  289
<212>  PRT
<213>  pig

<400>  18

Met Ala Ala Leu Ile Ala Glu Asn Phe Arg Phe Leu Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Gln Glu Leu Phe Thr Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Ala Arg Asn Tyr Leu Tyr Gly Leu Thr Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Ala Leu Phe Leu Ile Gly Val Ile Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Leu Val Ala Glu Cys Gln Tyr Arg Arg Thr Lys Asn Cys Ser Ala 
                85                  90                  95      


Ala Pro Asn Phe Leu Leu Leu Ser Ser Ile Val Gly Arg Ala Ala Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Leu Ser Glu Phe Val Asp Pro Ser Ser Leu Thr Ala Gly 
    130                 135                 140                 


Lys Glu Ser Phe Pro Leu Ala His Ala Thr Glu Ile Leu Ala Arg Phe 
145                 150                 155                 160 


Pro Cys Gly Glu Gly Pro Ala Asn Leu Ser Gly Phe Arg Glu Glu Val 
                165                 170                 175     


Ser Arg Arg Leu Lys Tyr Glu Ser Gln Leu Phe Gly Trp Leu Leu Ile 
            180                 185                 190         


Gly Val Val Ala Ile Leu Val Phe Leu Thr Lys Cys Leu Lys Gln Leu 
        195                 200                 205             


Phe Gln Arg Thr Ala Glu Val His Ser Arg Val Leu Ala Ala Asn Asn 
    210                 215                 220                 


Val Arg Arg Phe Phe Gly Phe Val Ala Leu Asn Lys Asp Asp Glu Glu 
225                 230                 235                 240 


Leu Val Ala Lys Phe Pro Val Glu Gly Thr Gln Pro Leu Tyr Arg Glu 
                245                 250                 255     


Asn Gln Gly Leu Pro Leu Tyr Ser Arg Leu His Lys Trp Ala Gln Gly 
            260                 265                 270         


Leu Ala Gly Asn Gly Thr Ala Pro Asp Asn Ile Glu Met Ala Leu Leu 
        275                 280                 285             


Thr 
    


<210>  19
<211>  322
<212>  PRT
<213>  human

<400>  19

Met Ala Ala Leu Ile Ala Glu Asn Phe Arg Phe Leu Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Gln Glu Leu Phe Ser Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Ala Arg Asn Tyr Leu Tyr Gly Leu Ala Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Val Leu Phe Ile Ile Gly Ile Ile Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Leu Val Ala Glu Cys Gln His Arg Arg Thr Lys Asn Cys Ser Ala 
                85                  90                  95      


Ala Pro Thr Phe Leu Leu Leu Ser Ser Ile Leu Gly Arg Ala Ala Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Leu Ser Glu Phe Val Asp Pro Ser Ser Leu Thr Ala Arg 
    130                 135                 140                 


Glu Glu His Phe Pro Ser Ala His Ala Thr Glu Ile Leu Ala Arg Phe 
145                 150                 155                 160 


Pro Cys Lys Glu Asn Pro Asp Asn Leu Ser Asp Phe Arg Glu Glu Val 
                165                 170                 175     


Ser Arg Arg Leu Arg Tyr Glu Ser Gln Leu Phe Gly Trp Leu Leu Ile 
            180                 185                 190         


Gly Val Val Ala Ile Leu Val Phe Leu Thr Lys Cys Leu Lys His Tyr 
        195                 200                 205             


Cys Ser Pro Leu Ser Tyr Arg Gln Glu Ala Tyr Trp Ala Gln Tyr Arg 
    210                 215                 220                 


Ala Asn Glu Asp Gln Leu Phe Gln Arg Thr Ala Glu Val His Ser Arg 
225                 230                 235                 240 


Val Leu Ala Ala Asn Asn Val Arg Arg Phe Phe Gly Phe Val Ala Leu 
                245                 250                 255     


Asn Lys Asp Asp Glu Glu Leu Ile Ala Asn Phe Pro Val Glu Gly Thr 
            260                 265                 270         


Gln Pro Arg Pro Gln Trp Asn Ala Ile Thr Gly Val Tyr Leu Val Arg 
        275                 280                 285             


Glu Asn Gln Gly Leu Pro Leu Tyr Ser Arg Leu His Lys Trp Ala Gln 
    290                 295                 300                 


Gly Leu Ala Gly Asn Gly Ala Ala Pro Asp Asn Tyr Glu Met Ala Leu 
305                 310                 315                 320 


Leu Pro 
        


<210>  20
<211>  318
<212>  PRT
<213>  chicken

<400>  20

Met Ala Ala Leu Ile Ala Glu Asn Phe Arg Phe Leu Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Glu Glu Leu Phe Ser Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Ala Arg Asn Tyr Ile Tyr Gly Leu Ala Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Ala Leu Phe Leu Ile Gly Val Ile Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Val Val Ala Glu Cys His Lys Arg Gly Thr Lys Asn Phe Ser Thr 
                85                  90                  95      


Ala Ala Thr Phe Leu Leu Phe Gly Ser Ile Met Gly Arg Ala Ala Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Ile Cys Ala Leu Ser Glu Phe Val Arg Pro Ser Thr Leu Asp Lys Phe 
    130                 135                 140                 


Pro Ser Glu Phe Gly Ala Glu Val Leu Ala Arg Phe Pro Cys Lys Asp 
145                 150                 155                 160 


Val Pro Ala Asn Leu Thr Lys Phe Arg Asp Glu Val Thr Arg Arg Leu 
                165                 170                 175     


Arg Tyr Glu Ser Gln Leu Phe Gly Trp Leu Leu Ile Gly Ile Val Ala 
            180                 185                 190         


Val Leu Val Phe Leu Thr Lys Cys Leu Lys His Cys Cys Ser Pro Leu 
        195                 200                 205             


Ser Tyr Arg Gln Glu Ala Tyr Trp Ala Gln Tyr Arg Ser Asn Glu Asp 
    210                 215                 220                 


Lys Leu Phe Arg Arg Thr Ala Glu Val His Ser Arg Ile Leu Ala Ala 
225                 230                 235                 240 


Lys Asn Val Lys Ala Phe Phe Gly Phe Val Ala Leu Asp Lys Glu Glu 
                245                 250                 255     


Lys Glu Leu Val Gln Glu Phe Pro Val Glu Gly Val Gln Pro Ser Pro 
            260                 265                 270         


Gln Trp Asn Ala Ile Thr Gly Val Tyr Ile Tyr Arg Glu Asn Lys Gly 
        275                 280                 285             


Phe Pro Leu Tyr Ser Arg Leu His Lys Trp Ala Lys Gly Val Glu Gly 
    290                 295                 300                 


Asn Gly Pro Thr Pro Glu Gly His Glu Met Leu Phe Leu Ala 
305                 310                 315             


<210>  21
<211>  318
<212>  PRT
<213>  frog

<400>  21

Met Ala Ser Ile Ile Thr Glu Asn Leu Lys Phe Phe Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Val Ala Leu Gly Thr 
            20                  25                  30          


Val Gly Ser Gln Glu Leu Phe Ser Val Val Ala Phe His Cys Pro Cys 
        35                  40                  45              


Ser Pro Gly Arg Asn Tyr Met Tyr Gly Leu Ala Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Val Leu Phe Leu Ile Gly Ile Met Leu Asn Asn His Thr Trp 
65                  70                  75                  80  


Asn Leu Val Ala Glu Cys Gln Lys Arg Ala Leu Lys Asn Cys Ser Thr 
                85                  90                  95      


Pro Ala Thr Phe Leu Leu Phe Gly Ser Ile Leu Gly Arg Ala Leu Val 
            100                 105                 110         


Ala Pro Val Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Arg Ser Glu Phe Leu Asn Pro Thr Ala Phe Leu Asp Phe 
    130                 135                 140                 


Pro Ser Glu Tyr Gly Pro Asp Ser Met Ala Arg Phe Ser Cys Pro Asp 
145                 150                 155                 160 


Thr Pro Lys Glu Leu Ile Pro Phe Lys Asp Glu Val Ile Arg Arg Leu 
                165                 170                 175     


Lys Tyr Glu Ser Gln Phe Leu Gly Trp Ile Leu Ile Ala Val Ile Ala 
            180                 185                 190         


Ser Ala Ala Phe Leu Leu Lys Cys Leu Gln His Cys Cys Thr Pro Leu 
        195                 200                 205             


Ser Phe His Gln Glu Asp Tyr Trp Lys Gln Tyr Arg Tyr Ala Glu Lys 
    210                 215                 220                 


Asp Leu Phe Asn Arg Thr Ala Glu Val His Ala Lys Val Leu Ala Ala 
225                 230                 235                 240 


Asn Asn Val Lys Ala Phe Phe Gly Phe Val Ala Leu Asp Lys Asp Glu 
                245                 250                 255     


Lys Asp Ile Val Ser Gln Tyr Pro Val Asp Glu Ala Gln Thr Ser Pro 
            260                 265                 270         


Gln Trp Gln Glu Ile Thr Gly Val Tyr Leu Tyr Arg Glu Asn Lys Gly 
        275                 280                 285             


Phe Pro Leu Tyr Ser Arg Leu His Lys Trp Thr Lys Lys Val Ile Gly 
    290                 295                 300                 


Asn Gly Met Asp Pro Asp Gly Arg Glu Met Ala Leu Leu Ala 
305                 310                 315             


<210>  22
<211>  310
<212>  PRT
<213>  zebrafish

<400>  22

Met Ala Ala Leu Ile Ser Glu Asn Phe Lys Phe Val Ser Leu Phe Phe 
1               5                   10                  15      


Lys Ser Lys Asp Val Met Ile Phe Asn Gly Leu Ile Gly Leu Gly Thr 
            20                  25                  30          


Val Ala Ser Gln Thr Ala Tyr Asn Ile Phe Ala Phe Asn Cys Pro Cys 
        35                  40                  45              


Ser Pro Lys Lys Asn Tyr Leu Tyr Gly Met Ala Ala Ile Gly Val Pro 
    50                  55                  60                  


Ala Leu Thr Phe Phe Val Ile Gly Leu Ile Leu Asn Arg Ser Thr Trp 
65                  70                  75                  80  


Asp Leu Val Ser Glu Cys Arg Thr Arg Gly Cys Arg Lys Leu Thr Leu 
                85                  90                  95      


Thr Ala Ala Ser Val Leu Met Gly Ser Ile Met Gly Arg Ala Ile Val 
            100                 105                 110         


Ala Pro Ile Thr Trp Ser Val Ile Ser Leu Leu Arg Gly Glu Ala Tyr 
        115                 120                 125             


Val Cys Ala Phe Ser Glu Phe Val Asp Pro Ser Thr Leu Asp His Phe 
    130                 135                 140                 


Pro Ile Thr Thr Lys Thr Val Glu Ile Met Ala Gln Phe Pro Cys Lys 
145                 150                 155                 160 


Asp Val Pro Asp Pro Tyr Thr Ile Tyr Thr Lys Val Ile Glu Arg Gln 
                165                 170                 175     


Leu Gln Tyr Glu Ser Gln Leu Leu Gly Trp Leu Leu Val Gly Ile Val 
            180                 185                 190         


Ser Leu Ser Val Phe Leu Leu Leu Cys Leu Lys Ser Cys Cys Ser Thr 
        195                 200                 205             


Leu Gly Tyr Gln Gln Glu Ala Tyr Trp Thr Gln Tyr Arg Ala Asn Glu 
    210                 215                 220                 


Arg Ala Ile Phe Gln Arg Thr Ala Glu Ile His Ala Lys Tyr Asn Ala 
225                 230                 235                 240 


Ala Asp Cys Val Lys Asn Phe Phe Gly Phe Val Ala Leu Glu Asn Glu 
                245                 250                 255     


Glu Lys Glu Val Leu Ala Thr Cys Asn Gly Ile Lys Ser Val Ile Pro 
            260                 265                 270         


Arg Leu Glu Trp Asn Arg Val Thr Gly Val Cys Leu Tyr Arg Glu Val 
        275                 280                 285             


Glu Asn Thr Pro Leu Tyr Ser Arg Leu Asn Lys Trp Asp Leu Tyr Ser 
    290                 295                 300                 


Lys Asp Phe Gly Asp Cys 
305                 310 


