                         SEQUENCE LISTING

<110>  SeNA Research, Incorporated
       Huang, Zhen
 
<120>  MODIFIED NUCLEOTIDES AND METHODS FOR DNA AND RNA POLYMERIZATION 
       AND SEQUENCING

<130>  17756.3WOU1

<140>  PCT/US2020/028110
<141>  2020-04-14

<150>  US 62/835,240
<151>  2019-04-17

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  1
gtcgagtcaa gagcatcc                                                     18


<210>  2
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  2
cagctcagtt ctcgtaggtt cagctaggtc agtcacatga gtc                         43


<210>  3
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  3
gtcgagtcaa gagcatccaa gtcgatccag tcagtgtact cag                         43


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  4
cagctcagtt ctcgtaggtc agacag                                            26


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  5
cagctcagtt ctcgtagggt catcta                                            26


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  6
cagctcagtt ctcgtaggca tgtatg                                            26


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  7
cagctcagtt ctcgtaggaa tgtctg                                            26


<210>  8
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  8
acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat cctctagagt       60

cgacctgcag gcatgcaagc ttggcgtaat catggtcat                              99


<210>  9
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  9
aatttcacac aggaaacagc tatgaccatg attacgcc                               38


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence


<220>
<221>  modified_base
<222>  (1)..(21)
<223>  seleno modified nucleotides, each nucleotide in the series is a 
       non-natural nucleotide (having an Se instead of O on the first 
       phosphate group of each nucleotide)

<400>  10
dagtactaga tgtgagacat c                                                 21


<210>  11
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  11
ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc gtcgttttac       60

aacgtcg                                                                 67


<210>  12
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  12
agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa       60

at                                                                      62


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  13
gtcgagtcaa gagcatcc                                                     18


<210>  14
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  14
cagctcagtt ctcgtaggtc agacag                                            26


<210>  15
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  15
cagctcagtt ctcgtagggt catcta                                            26


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  16
cagctcagtt ctcgtaggca tgtatg                                            26


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  17
cagctcagtt ctcgtaggaa tgtctg                                            26


<210>  18
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  18
aacagctcct                                                              10


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  19
ttgtcgagga gcgggaacga gtggta                                            26


<210>  20
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  20
gcgggaacga gtggtattgt cgatttgga                                         29


<210>  21
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  21
gcgggaacga gtggtattgt cgagga                                            26


<210>  22
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  22
tcaagatccg ccacaacatc ctgggtgctc aggtagtg                               38


<210>  23
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  23
cgacaagcag aagaacggca tcgcgcttct cgttggggt                              39


<210>  24
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  24
aagatgcgac ccgattcaag cttgcagttg atcctggtgg                             40


<210>  25
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  25
tgcaggctga actggtggcc tccactgagc agcttg                                 36


<210>  26
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  26
ttggagaggt catcaggaag ggttggctac ttccagtttt actcc                       45


<210>  27
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  27
tgcaggctga actggtggcc tccactgagc agcttg                                 36


<210>  28
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  28
gcaatgagct catggcttcc tttttaagaa gaggttttct ggg                         43


<210>  29
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  29
cgtcttggcc                                                              10


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  30
gcagaaccgg atgtcgtccg tcggcgcttg                                        30


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq32

<400>  31
gcagaaccgg tcgcttcctc ttc                                               23


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  32
gcagaaccgg tcggcttcct cttc                                              24


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  33
gcagaaccgg tcgggcttcc tcttc                                             25


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA sequence and provided for demonstrating the ability
       to reproduce DNA sequences more faithfully using PCR, by 
       incorporating selenium-modified nucleotides into the sequence

<400>  34
gcagaaccgg atgtcgtccg tcggcgcttg                                        30


