                         SEQUENCE LISTING

<110>  Arizona Board of Regents on behalf of Arizona State 
       University
 
<120>  Cas9 Fusion Proteins and Related Methods

<130>  11157.044PCT

<150>  62/834,880
<151>  2019-04-16

<160>  121   

<170>  PatentIn version 3.5

<210>  1
<211>  1556
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  1

Met Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Met Arg Ile Phe Gly 
1               5                   10                  15      


Tyr Ala Arg Val Ser Thr Ser Gln Gln Ser Leu Asp Ile Gln Ile Arg 
            20                  25                  30          


Ala Leu Lys Asp Ala Gly Val Lys Ala Asn Arg Ile Phe Thr Asp Lys 
        35                  40                  45              


Ala Ser Gly Ser Ser Thr Asp Arg Glu Gly Leu Asp Leu Leu Arg Met 
    50                  55                  60                  


Lys Val Glu Glu Gly Asp Val Ile Leu Val Lys Lys Leu Asp Arg Leu 
65                  70                  75                  80  


Ser Arg Asp Thr Ala Asp Met Ile Gln Leu Ile Lys Glu Phe Asp Ala 
                85                  90                  95      


Gln Gly Val Ala Val Arg Phe Ile Asp Asp Gly Ile Ser Thr Asp Gly 
            100                 105                 110         


Tyr Met Gly Gln Met Val Val Thr Ile Leu Ser Ala Val Ala Gln Ala 
        115                 120                 125             


Glu Arg Arg Arg Ile Leu Gln Arg Thr Asn Glu Gly Arg Gln Glu Ala 
    130                 135                 140                 


Lys Leu Lys Gly Ile Lys Phe Gly Arg Arg Arg Thr Val Asp Arg Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
                165                 170                 175     


Ser Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser 
            180                 185                 190         


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        195                 200                 205             


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    210                 215                 220                 


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
225                 230                 235                 240 


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                245                 250                 255     


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            260                 265                 270         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        275                 280                 285             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    290                 295                 300                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
305                 310                 315                 320 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                325                 330                 335     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            340                 345                 350         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        355                 360                 365             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    370                 375                 380                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
385                 390                 395                 400 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                405                 410                 415     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            420                 425                 430         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        435                 440                 445             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    450                 455                 460                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
465                 470                 475                 480 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                485                 490                 495     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            500                 505                 510         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        515                 520                 525             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    530                 535                 540                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
545                 550                 555                 560 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                565                 570                 575     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            580                 585                 590         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        595                 600                 605             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    610                 615                 620                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
625                 630                 635                 640 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                645                 650                 655     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            660                 665                 670         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        675                 680                 685             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    690                 695                 700                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
705                 710                 715                 720 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                725                 730                 735     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            740                 745                 750         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        755                 760                 765             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    770                 775                 780                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
785                 790                 795                 800 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                805                 810                 815     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            820                 825                 830         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        835                 840                 845             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    850                 855                 860                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
865                 870                 875                 880 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                885                 890                 895     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            900                 905                 910         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        915                 920                 925             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    930                 935                 940                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
945                 950                 955                 960 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                965                 970                 975     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            980                 985                 990         


Leu Gln Asn Gly Arg Asp Met Tyr  Val Asp Gln Glu Leu  Asp Ile Asn 
        995                 1000                 1005             


Arg Leu  Ser Asp Tyr Asp Val  Asp Ala Ile Val Pro  Gln Ser Phe 
    1010                 1015                 1020             


Leu Lys  Asp Asp Ser Ile Asp  Asn Lys Val Leu Thr  Arg Ser Asp 
    1025                 1030                 1035             


Lys Asn  Arg Gly Lys Ser Asp  Asn Val Pro Ser Glu  Glu Val Val 
    1040                 1045                 1050             


Lys Lys  Met Lys Asn Tyr Trp  Arg Gln Leu Leu Asn  Ala Lys Leu 
    1055                 1060                 1065             


Ile Thr  Gln Arg Lys Phe Asp  Asn Leu Thr Lys Ala  Glu Arg Gly 
    1070                 1075                 1080             


Gly Leu  Ser Glu Leu Asp Lys  Ala Gly Phe Ile Lys  Arg Gln Leu 
    1085                 1090                 1095             


Val Glu  Thr Arg Gln Ile Thr  Lys His Val Ala Gln  Ile Leu Asp 
    1100                 1105                 1110             


Ser Arg  Met Asn Thr Lys Tyr  Asp Glu Asn Asp Lys  Leu Ile Arg 
    1115                 1120                 1125             


Glu Val  Lys Val Ile Thr Leu  Lys Ser Lys Leu Val  Ser Asp Phe 
    1130                 1135                 1140             


Arg Lys  Asp Phe Gln Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr 
    1145                 1150                 1155             


His His  Ala His Asp Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala 
    1160                 1165                 1170             


Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly 
    1175                 1180                 1185             


Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu 
    1190                 1195                 1200             


Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn 
    1205                 1210                 1215             


Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu 
    1220                 1225                 1230             


Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu 
    1235                 1240                 1245             


Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val 
    1250                 1255                 1260             


Leu Ser  Met Pro Gln Val Asn  Ile Val Lys Lys Thr  Glu Val Gln 
    1265                 1270                 1275             


Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser 
    1280                 1285                 1290             


Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr 
    1295                 1300                 1305             


Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr Ser Val  Leu Val Val 
    1310                 1315                 1320             


Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys 
    1325                 1330                 1335             


Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys 
    1340                 1345                 1350             


Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys 
    1355                 1360                 1365             


Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1370                 1375                 1380             


Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln 
    1385                 1390                 1395             


Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu 
    1400                 1405                 1410             


Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp 
    1415                 1420                 1425             


Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln His Lys  His Tyr Leu 
    1430                 1435                 1440             


Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile 
    1445                 1450                 1455             


Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys 
    1460                 1465                 1470             


His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His 
    1475                 1480                 1485             


Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr 
    1490                 1495                 1500             


Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu 
    1505                 1510                 1515             


Val Leu  Asp Ala Thr Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr 
    1520                 1525                 1530             


Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly Gly Asp  Ser Arg Ala 
    1535                 1540                 1545             


Asp Pro  Lys Lys Lys Arg Lys  Val 
    1550                 1555     


<210>  2
<211>  4671
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  2
atgcccaaaa agaagaggaa agtaggtggc agtatgagga tatttggata cgcacgagtt       60

agtacatctc aacagtctct ggacatacag ataagggcat tgaaggatgc tggagtgaaa      120

gctaatcgga tctttaccga taaggctagt ggcagctcta cagacagaga gggcttggat      180

ctgctgagaa tgaaggttga ggaaggggat gtgatactcg tcaagaaact tgacagactc      240

tcacgagaca ctgccgatat gatccaactg attaaggaat ttgacgccca aggtgtagcc      300

gtaagattta ttgatgacgg tatcagcacc gacggctata tgggccagat ggtcgtcact      360

atactgagcg ctgtcgcaca agcggagaga agacgaatcc tgcaacggac aaatgaaggt      420

cgccaagaag caaagctcaa aggaattaag ttcgggcgca ggaggactgt agacagaggt      480

ggttcaggag gaagtggagg ttcaggggga agtggtggct ccggtgggtc tatggacaag      540

aagtactcca ttgggctcgc catcggcaca aacagcgtcg gttgggccgt cattacggac      600

gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata      660

aagaagaacc tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg      720

ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag      780

gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag      840

tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg      900

gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta      960

gacagtactg ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa     1020

tttcggggac acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa     1080

ctctttatcc aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca     1140

tccggagttg acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa     1200

aacctcatcg cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc     1260

ctgtcactcg ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag     1320

cttcaactga gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc     1380

gaccagtacg cagacctttt tttggcggca aagaacctgt cagacgccat tctgctgagt     1440

gatattctgc gagtgaacac ggagatcacc aaagctccgc tgagcgctag tatgatcaag     1500

cgctatgatg agcaccacca agacttgact ttgctgaagg cccttgtcag acagcaactg     1560

cctgagaagt acaaggaaat tttcttcgat cagtctaaaa atggctacgc cggatacatt     1620

gacggcggag caagccagga ggaattttac aaatttatta agcccatctt ggaaaaaatg     1680

gacggcaccg aggagctgct ggtaaagctt aacagagaag atctgttgcg caaacagcgc     1740

actttcgaca atggaagcat cccccaccag attcacctgg gcgaactgca cgctatcctc     1800

aggcggcaag aggatttcta cccctttttg aaagataaca gggaaaagat tgagaaaatc     1860

ctcacatttc ggatacccta ctatgtaggc cccctcgccc ggggaaattc cagattcgcg     1920

tggatgactc gcaaatcaga agagaccatc actccctgga acttcgagga agtcgtggat     1980

aagggggcct ctgcccagtc cttcatcgaa aggatgacta actttgataa aaatctgcct     2040

aacgaaaagg tgcttcctaa acactctctg ctgtacgagt acttcacagt ttataacgag     2100

ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc cagcattcct gtctggagag     2160

cagaagaaag ctatcgtgga cctcctcttc aagacgaacc ggaaagttac cgtgaaacag     2220

ctcaaagaag actatttcaa aaagattgaa tgtttcgact ctgttgaaat cagcggagtg     2280

gaggatcgct tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat cattaaagac     2340

aaggacttcc tggacaatga ggagaacgag gacattcttg aggacattgt cctcaccctt     2400

acgttgtttg aagataggga gatgattgaa gaacgcttga aaacttacgc tcatctcttc     2460

gacgacaaag tcatgaaaca gctcaagagg cgccgatata caggatgggg gcggctgtca     2520

agaaaactga tcaatgggat ccgagacaag cagagtggaa agacaatcct ggattttctt     2580

aagtccgatg gatttgccaa ccggaacttc atgcagttga tccatgatga ctctctcacc     2640

tttaaggagg acatccagaa agcacaagtt tctggccagg gggacagtct tcacgagcac     2700

atcgctaatc ttgcaggtag cccagctatc aaaaagggaa tactgcagac cgttaaggtc     2760

gtggatgaac tcgtcaaagt aatgggaagg cataagcccg agaatatcgt tatcgagatg     2820

gcccgagaga accaaactac ccagaaggga cagaagaaca gtagggaaag gatgaagagg     2880

attgaagagg gtataaaaga actggggtcc caaatcctta aggaacaccc agttgaaaac     2940

acccagcttc agaatgagaa gctctacctg tactacctgc agaacggcag ggacatgtac     3000

gtggatcagg aactggacat caatcggctc tccgactacg acgtggatgc catcgtgccc     3060

cagtcttttc tcaaagatga ttctattgat aataaagtgt tgacaagatc cgataaaaat     3120

agagggaaga gtgataacgt cccctcagaa gaagttgtca agaaaatgaa aaattattgg     3180

cggcagctgc tgaacgccaa actgatcaca caacggaagt tcgataatct gactaaggct     3240

gaacgaggtg gcctgtctga gttggataaa gccggcttca tcaaaaggca gcttgttgag     3300

acacgccaga tcaccaagca cgtggcccaa attctcgatt cacgcatgaa caccaagtac     3360

gatgaaaatg acaaactgat tcgagaggtg aaagttatta ctctgaagtc taagctggtc     3420

tcagatttca gaaaggactt tcagttttat aaggtgagag agatcaacaa ttaccaccat     3480

gcgcatgatg cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa atatcccaag     3540

cttgaatctg aatttgttta cggagactat aaagtgtacg atgttaggaa aatgatcgca     3600

aagtctgagc aggaaatagg caaggccacc gctaagtact tcttttacag caatattatg     3660

aattttttca agaccgagat tacactggcc aatggagaga ttcggaagcg accacttatc     3720

gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg gtagggattt cgcgacagtc     3780

cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt acagaccgga     3840

ggcttctcca aggaaagtat cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa     3900

aaagattggg accccaagaa atacggcgga ttcgattctc ctacagtcgc ttacagtgta     3960

ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg     4020

ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag     4080

gcgaaaggat ataaagaggt caaaaaagac ctcatcatta agcttcccaa gtactctctc     4140

tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt     4200

aacgagctgg cactgccctc taaatacgtt aatttcttgt atctggccag ccactatgaa     4260

aagctcaaag ggtctcccga agataatgag cagaagcagc tgttcgtgga acaacacaaa     4320

cactaccttg atgagatcat cgagcaaata agcgaattct ccaaaagagt gatcctcgcc     4380

gacgctaacc tcgataaggt gctttctgct tacaataagc acagggataa gcccatcagg     4440

gagcaggcag aaaacattat ccacttgttt actctgacca acttgggcgc gcctgcagcc     4500

ttcaagtact tcgacaccac catagacaga aagcggtaca cctctacaaa ggaggtcctg     4560

gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat cgacctctct     4620

cagctcggtg gagacagcag ggctgacccc aagaagaaga ggaaggtgtg a              4671


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  3
agaagagcga gctcttct                                                     18


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  4
cgaacgtacg agtgcaagcc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  5
gaacgtacga gtgcaagcct                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  6
aacgtacgag tgcaagcctg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  7
acgtacgagt gcaagcctgg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  8
acgagtgcaa gcctggggga                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  9
tgcaagcctg ggggatggat                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  10
cagacagacc atactccaga                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  11
agacagacca tactccagat                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  12
gacagaccat actccagatg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  13
acagaccata ctccagatgg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  14
accatactcc agatggggga                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  15
actccagatg ggggatggct                                                   20


<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  16
gggtcttcga gaagacct                                                     18


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  17
gacgtacgag tgcaagcctg g                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  18
gacagacctt actccagaag g                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  19
gtgcaagcct gggggaagga t                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  20
gactccagaa gggggaaggc t                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  21
gacgagtgca agcctggggg a                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  22
gaccttactc cagaaggggg a                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  23
gttgctcacc atggtggcga c                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
gctcgccctt gctcaccatg g                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  25
gctcctcgcc cttgctcacc a                                                 21


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  26
ggtcgccacc atggtgagca                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  27
gtcgccacca tggtgagcaa                                                   20


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA guide sequence

<400>  28
gcaccatggt gagcaagggc g                                                 21


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
cgcatatgtg gtgttgaaga                                                   20


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
ctagggcttt ctgctctgtc at                                                22


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
tggagggcac agttaagccg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
aataccgcct ttgagtgagc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
agctgtgacc ggcgcctacg                                                   20


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
ctgagcaccc agtccgccct gag                                               23


<210>  35
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
aattctccga tccatccccc aggcttgcac tcgtacgttc gaaatat                     47


<210>  36
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
ataatatttc gaacgtacga gtgcaagcct gggggatgga tcggag                      46


<210>  37
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
tataaattat cagacagacc atactccaga tgggggatgg ctaggtg                     47


<210>  38
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
aattcaccta gccatccccc atctggagta tggtctgtct gataattt                    48


<210>  39
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
cgcgttccga tccatccccc aggcttgcac tcgtacgttc gaaatat                     47


<210>  40
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
ataatatttc gaacgtacga gtgcaagcct gggggatgga tcggaa                      46


<210>  41
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
tataaattat cagacagacc atactccaga tgggggatgg ctaggta                     47


<210>  42
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
cgcgtaccta gccatccccc atctggagta tggtctgtct gataattt                    48


<210>  43
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
aattctccga tccttccccc aggcttgcac tcgtacgttc gaaatat                     47


<210>  44
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
ctccgatcct tcccccaggc ttgcactcgt acgttcgaaa tat                         43


<210>  45
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
ataatatttc gaacgtacga gtgcaagcct gggggaagga tcggag                      46


<210>  46
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
tataaattat cagacagacc ttactccaga agggggaagg ctaggtg                     47


<210>  47
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
gatccaccta gccttccccc ttctggagta aggtctgtct gataattt                    48


<210>  48
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
cacctagcct tcccccttct ggagtaaggt ctgtctgata attt                        44


<210>  49
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
agaacagttg atagaggagg gagcggggga agcggtggct ca                          42


<210>  50
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  50
cattgagcca ccgcttcccc cgctccctcc tctatcaact gt                          42


<210>  51
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  51
agaactgttg accgaggtgg ttcaggagga agtgga                                 36


<210>  52
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  52
acctccactt cctcctgaac cacctcggtc aacagt                                 36


<210>  53
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  53
ggttcagggg gaagtggtgg ctccggtggg tct                                    33


<210>  54
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  54
catagaccca ccggagccac cacttccccc tga                                    33


<210>  55
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  55
agaacagttg atcggagcgg ttctgagact                                        30


<210>  56
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  56
cggagtctca gaaccgctcc gatcaactgt                                        30


<210>  57
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  57
ccgggaacct cagagtctgc tacgccggaa agc                                    33


<210>  58
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  58
catgctttcc ggcgtagcag actctgaggt tcc                                    33


<210>  59
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  59
agaaccgtag atcgcggggg ctctggagga tcaggta                                37


<210>  60
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  60
cgctacctga tcctccagag cccccgcgat ctacggt                                37


<210>  61
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  61
gcgaaacgcc gggtactagc gaaagcgcga cacctgagag t                           41


<210>  62
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  62
catactctca ggtgtcgcgc tttcgctagt acccggcgtt t                           41


<210>  63
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  63
acggctcttc gatgcccaaa aagaagagga aagt                                   34


<210>  64
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  64
agcgctcttc atctgtctac agtcctcctg cg                                     32


<210>  65
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  65
acggctcttc gcattttttt cccgggggat cc                                     32


<210>  66
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  66
gcgctcttca gccatcggca caaacagcg                                         29


<210>  67
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  67
acggctcttc gggcgagccc aatggagtac ttctt                                  35


<210>  68
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  68
gcgctcttca gccatcgtgc cccagtcttt t                                      31


<210>  69
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  69
acggctcttc gggcatccac gtcgtagtcg gag                                    33


<210>  70
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  70
atacaccggt gccaccatgc ccaaaaagaa gaggaaagt                              39


<210>  71
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  71
gatgacaatt gtcacacctt cctcttcttc ttg                                    33


<210>  72
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  72
gtgagaattc tctttgaaaa gataatgtat gattatgc                               38


<210>  73
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  73
acggctcttc gtctttgaaa agataatgta tgattatgc                              39


<210>  74
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  74
acggctcttc gagagtctcc aattatctag taaaaaaagc acc                         43


<210>  75
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  75
cgtcatgtca ctagtagagt ctccaattat ctagtaaaaa aagcacc                     47


<210>  76
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  76
gtgagaattc gagggcctat ttcccatgat                                        30


<210>  77
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  77
acggctcttc gtctgtctgc agaattggcg                                        30


<210>  78
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  78
agcgctcttc tagagagggc ctatttccca tgat                                   34


<210>  79
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  79
cgtcatgtct ctagatttgt ctgcagaatt ggcg                                   34


<210>  80
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  80
atccgaacgt acgagtgcaa gcc                                               23


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  81
aacggcttgc actcgtacgt tcg                                               23


<210>  82
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  82
atcgaacgta cgagtgcaag cct                                               23


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  83
aacaggcttg cactcgtacg ttc                                               23


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  84
atcaacgtac gagtgcaagc ctg                                               23


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  85
aaccaggctt gcactcgtac gtt                                               23


<210>  86
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  86
atcacgtacg agtgcaagcc tgg                                               23


<210>  87
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  87
aacccaggct tgcactcgta cgt                                               23


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  88
atcacgagtg caagcctggg gga                                               23


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  89
aactccccca ggcttgcact cgt                                               23


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  90
atctgcaagc ctgggggatg gat                                               23


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  91
aacatccatc ccccaggctt gca                                               23


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  92
atccagacag accatactcc aga                                               23


<210>  93
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  93
aactctggag tatggtctgt ctg                                               23


<210>  94
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  94
atcagacaga ccatactcca gat                                               23


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  95
aacatctgga gtatggtctg tct                                               23


<210>  96
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  96
atcgacagac catactccag atg                                               23


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  97
aaccatctgg agtatggtct gtc                                               23


<210>  98
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  98
atcacagacc atactccaga tgg                                               23


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  99
aacccatctg gagtatggtc tgt                                               23


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  100
atcaccatac tccagatggg gga                                               23


<210>  101
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  101
aactccccca tctggagtat ggt                                               23


<210>  102
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  102
atcactccag atgggggatg gct                                               23


<210>  103
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  103
aacagccatc ccccatctgg agt                                               23


<210>  104
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  104
caccgacgta cgagtgcaag cctgg                                             25


<210>  105
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  105
aaacccaggc ttgcactcgt acgtc                                             25


<210>  106
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  106
caccgacaga ccttactcca gaagg                                             25


<210>  107
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  107
aaacccttct ggagtaaggt ctgtc                                             25


<210>  108
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  108
caccgtgcaa gcctggggga aggat                                             25


<210>  109
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  109
aaacatcctt cccccaggct tgcac                                             25


<210>  110
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  110
caccgactcc agaaggggga aggct                                             25


<210>  111
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  111
aaacagcctt cccccttctg gagtc                                             25


<210>  112
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  112
caccgacgag tgcaagcctg gggga                                             25


<210>  113
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  113
aaactccccc aggcttgcac tcgtc                                             25


<210>  114
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  114
caccgacctt actccagaag gggga                                             25


<210>  115
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  115
aaactccccc ttctggagta aggtc                                             25


<210>  116
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  iCas9 site

<400>  116
tccgatccat cccccaggct tgcactcgta cgttcgaaat attataaatt atcagacaga       60

ccatactcca gatgggggat ggctaggt                                          88


<210>  117
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  iCas9 site

<400>  117
tccgatcctt cccccaggct tgcactcgta cgttcgaaat attataaatt atcagacaga       60

ccttactcca gaagggggaa ggctaggt                                          88


<210>  118
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  iCas9 site with accessory site

<400>  118
tccgatcctt cccccaggct tgcactcgta cgttcgaaat attataaatt atcagacaga       60

ccttactcca gaagggggaa ggctaggtgg ctaccggtcg ccaccatggt gagcaagggc      120

gag                                                                    123


<210>  119
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  iCas9 site with accessory site

<400>  119
tccgatcctt cccccaggct tgcactcgta cgttcgaaat attataaatt atcagacaga       60

ccttactcca gaagggggaa ggctaggtgg ctaccggtcg ccaccatggt gagcaagggc      120

gag                                                                    123


<210>  120
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  120

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


<210>  121
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  121

Gly Gly Ser Gly Gly Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
1               5                   10                  15      


Ala Thr Pro Glu Ser 
            20      


