                         SEQUENCE LISTING

<110>  йž¿ѧԺҽѧоԺ
 
<120>  һֿװΪ׿ںϵ׼Ӧ

<130>  IEC190016PCT

<150>  201910352203.X
<151>  2019-04-29

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  103
<212>  PRT
<213>  ˹

<220>
<223>  CTBİ

<400>  1

Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr Gln 
1               5                   10                  15      


Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala 
            20                  25                  30          


Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Ile Phe 
        35                  40                  45              


Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala 
    50                  55                  60                  


Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala 
65                  70                  75                  80  


Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala Ile 
                85                  90                  95      


Ala Ala Ile Ser Met Ala Asn 
            100             


<210>  2
<211>  309
<212>  DNA
<213>  ˹

<220>
<223>  CTBĺ

<400>  2
accccgcaga acatcaccga cctgtgcgcc gagtaccaca acacccaaat ttataccctg       60

aacgacaaaa tttttagcta caccgagagc ctggcaggca agcgcgagat ggccatcatc      120

accttcaaga acggcgccat tttccaggtg gaggtgccgg gcagccagca catcgacagt      180

cagaagaagg ccatcgagcg catgaaggac accctgcgca tcgcctacct gaccgaggcc      240

aaggtggaga agctgtgcgt gtggaacaac aagaccccgc acgccatcgc cgcaatcagc      300

atggccaac                                                              309


<210>  3
<211>  88
<212>  PRT
<213>  ˹

<220>
<223>  StxBİ

<400>  3

Lys Lys Thr Leu Leu Ile Ala Ala Ser Leu Ser Phe Phe Ser Ala Ser 
1               5                   10                  15      


Ala Leu Ala Thr Pro Asp Cys Val Thr Gly Lys Val Glu Tyr Thr Lys 
            20                  25                  30          


Tyr Asn Asp Asp Asp Thr Phe Thr Val Lys Val Gly Asp Lys Glu Leu 
        35                  40                  45              


Phe Thr Asn Arg Trp Asn Leu Gln Ser Leu Leu Leu Ser Ala Gln Ile 
    50                  55                  60                  


Thr Gly Met Thr Val Thr Ile Lys Thr Asn Ala Cys His Asn Gly Gly 
65                  70                  75                  80  


Gly Phe Ser Glu Val Ile Phe Arg 
                85              


<210>  4
<211>  264
<212>  DNA
<213>  ˹

<220>
<223>  StxBĺ

<400>  4
aaaaaaacat tattaatagc tgcatcgctt tcattttttt cagcaagtgc gctggcgacg       60

cctgattgtg taactggaaa ggtggagtat acaaaatata atgatgacga tacctttaca      120

gttaaagtgg gtgataaaga attatttacc aacagatgga atcttcagtc tcttcttctc      180

agtgcgcaaa ttacggggat gactgtaacc attaaaacta atgcctgtca taatggaggg      240

ggattcagcg aagttatttt tcgt                                             264


<210>  5
<211>  103
<212>  PRT
<213>  ˹

<220>
<223>  LTBİ

<400>  5

Ala Pro Gln Thr Ile Thr Glu Leu Cys Ser Glu Tyr Arg Asn Thr Gln 
1               5                   10                  15      


Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr Thr Glu Ser Met Ala 
            20                  25                  30          


Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys Ser Gly Glu Thr Phe 
        35                  40                  45              


Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala 
    50                  55                  60                  


Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr Tyr Leu Thr Glu Thr 
65                  70                  75                  80  


Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys Thr Pro Asn Ser Ile 
                85                  90                  95      


Ala Ala Ile Ser Met Lys Asn 
            100             


<210>  6
<211>  309
<212>  DNA
<213>  ˹

<220>
<223>  LTBĺ

<400>  6
gcacctcaga ccattaccga actgtgcagt gaatatcgca atacccagat ctataccatt       60

aatgataaga ttctgagcta caccgaaagt atggccggta aacgcgaaat ggttattatt      120

acctttaaga gtggcgaaac ctttcaggtt gaagttccgg gtagccagca tattgatagt      180

cagaaaaaag caattgagcg catgaaagat accctgcgca ttacctatct gaccgaaacc      240

aaaattgata aactgtgcgt ttggaataac aaaaccccga atagcattgc agcaattagt      300

atgaaaaac                                                              309


<210>  7
<211>  140
<212>  PRT
<213>  ˹

<220>
<223>  SubBİ

<400>  7

Thr Ile Lys Arg Phe Phe Val Cys Ala Gly Ile Met Gly Cys Leu Ser 
1               5                   10                  15      


Leu Asn Pro Ala Met Ala Glu Trp Thr Gly Asp Ala Arg Asp Gly Met 
            20                  25                  30          


Phe Ser Gly Val Val Ile Thr Gln Phe His Thr Gly Gln Ile Asp Asn 
        35                  40                  45              


Lys Pro Tyr Phe Cys Ile Glu Gly Lys Gln Ser Ala Gly Ser Ser Ile 
    50                  55                  60                  


Ser Ala Cys Ser Met Lys Asn Ser Ser Val Trp Gly Ala Ser Phe Ser 
65                  70                  75                  80  


Thr Leu Tyr Asn Gln Ala Leu Tyr Phe Tyr Thr Thr Gly Gln Pro Val 
                85                  90                  95      


Arg Ile Tyr Tyr Glu Pro Gly Val Trp Thr Tyr Pro Pro Phe Val Lys 
            100                 105                 110         


Ala Leu Thr Ser Asn Ala Leu Val Gly Leu Ser Thr Cys Thr Thr Ser 
        115                 120                 125             


Thr Glu Cys Phe Gly Pro Asp Arg Lys Lys Asn Ser 
    130                 135                 140 


<210>  8
<211>  420
<212>  DNA
<213>  ˹

<220>
<223>  SubBĺ

<400>  8
accattaagc gctttttcgt gtgtgcaggc attatgggtt gtctgagcct gaatccggca       60

atggccgaat ggaccggcga tgcacgtgat ggcatgttta gcggtgttgt gattacccag      120

tttcataccg gtcagattga taataagccg tatttttgta tcgagggcaa acagagtgcc      180

ggtagtagca ttagtgcatg tagcatgaaa aatagcagtg tgtggggcgc cagctttagt      240

accctgtata atcaggcact gtatttttat accaccggtc agccggtgcg catctattat      300

gaaccgggcg tgtggaccta tccgccgttt gtgaaagcac tgaccagtaa tgccctggtt      360

ggcctgagca cctgcaccac cagcaccgaa tgttttggtc cggatcgtaa aaagaatagt      420


<210>  9
<211>  46
<212>  PRT
<213>  ˹

<220>
<223>  Triİ

<400>  9

Arg Leu Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu 
1               5                   10                  15      


Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu 
            20                  25                  30          


Glu Leu Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg 
        35                  40                  45      


<210>  10
<211>  138
<212>  PRT
<213>  ˹

<220>
<223>  Triĺ

<400>  10

Cys Gly Cys Cys Thr Gly Cys Thr Gly Cys Thr Gly Cys Gly Thr Cys 
1               5                   10                  15      


Thr Gly Gly Ala Ala Gly Ala Ala Cys Thr Gly Gly Ala Ala Cys Gly 
            20                  25                  30          


Thr Cys Gly Cys Cys Thr Gly Gly Ala Ala Gly Ala Gly Cys Thr Gly 
        35                  40                  45              


Gly Cys Ala Ala Ala Gly Thr Thr Cys Gly Thr Gly Gly Cys Ala Gly 
    50                  55                  60                  


Cys Ala Thr Gly Gly Ala Cys Cys Cys Thr Gly Ala Ala Ala Gly Cys 
65                  70                  75                  80  


Gly Gly Cys Thr Gly Cys Gly Gly Thr Thr Gly Ala Cys Cys Thr Gly 
                85                  90                  95      


Gly Ala Ala Cys Thr Gly Gly Cys Thr Gly Cys Thr Cys Thr Gly Cys 
            100                 105                 110         


Gly Thr Cys Gly Thr Cys Gly Thr Cys Thr Gly Gly Ala Gly Gly Ala 
        115                 120                 125             


Gly Cys Thr Gly Gly Cys Thr Cys Gly Thr 
    130                 135             


<210>  11
<211>  729
<212>  DNA
<213>  ˹

<220>
<223>  tacCTBTriеĺ

<400>  11
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaccccg      240

cagaacatca ccgacctgtg cgccgagtac cacaacaccc aaatttatac cctgaacgac      300

aaaattttta gctacaccga gagcctggca ggcaagcgcg agatggccat catcaccttc      360

aagaacggcg ccattttcca ggtggaggtg ccgggcagcc agcacatcga cagtcagaag      420

aaggccatcg agcgcatgaa ggacaccctg cgcatcgcct acctgaccga ggccaaggtg      480

gagaagctgt gcgtgtggaa caacaagacc ccgcacgcca tcgccgcaat cagcatggcc      540

aacggcggat ccggccgcct gctgctgcgt ctggaagaac tggaacgtcg cctggaagag      600

ctggcaaagt tcgtggcagc atggaccctg aaagcggctg cggttgacct ggaactggct      660

gctctgcgtc gtcgtctgga ggagctggct cgtggcggtt ctggtctcga gcaccaccac      720

caccaccac                                                              729


<210>  12
<211>  184
<212>  PRT
<213>  ˹

<220>
<223>  CTBTri׵İ

<400>  12

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His 
            20                  25                  30          


Asn Thr Gln Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu 
        35                  40                  45              


Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly 
    50                  55                  60                  


Ala Ile Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln 
65                  70                  75                  80  


Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu 
                85                  90                  95      


Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro 
            100                 105                 110         


His Ala Ile Ala Ala Ile Ser Met Ala Asn Gly Gly Ser Gly Arg Leu 
        115                 120                 125             


Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala Lys 
    130                 135                 140                 


Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu Leu 
145                 150                 155                 160 


Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser Gly 
                165                 170                 175     


Leu Glu His His His His His His 
            180                 


<210>  13
<211>  684
<212>  DNA
<213>  ˹

<220>
<223>  tacStxBTriеĺ

<400>  13
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaaaaaa      240

acattattaa tagctgcatc gctttcattt ttttcagcaa gtgcgctggc gacgcctgat      300

tgtgtaactg gaaaggtgga gtatacaaaa tataatgatg acgatacctt tacagttaaa      360

gtgggtgata aagaattatt taccaacaga tggaatcttc agtctcttct tctcagtgcg      420

caaattacgg ggatgactgt aaccattaaa actaatgcct gtcataatgg agggggattc      480

agcgaagtta tttttcgtgg cggatccggc cgcctgctgc tgcgtctgga agaactggaa      540

cgtcgcctgg aagagctggc aaagttcgtg gcagcatgga ccctgaaagc ggctgcggtt      600

gacctggaac tggctgctct gcgtcgtcgt ctggaggagc tggctcgtgg cggttctggt      660

ctcgagcacc accaccacca ccac                                             684


<210>  14
<211>  169
<212>  PRT
<213>  ˹

<220>
<223>  StxBTri׵İ

<400>  14

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Lys Lys Thr Leu Leu Ile Ala Ala Ser Leu Ser Phe Phe 
            20                  25                  30          


Ser Ala Ser Ala Leu Ala Thr Pro Asp Cys Val Thr Gly Lys Val Glu 
        35                  40                  45              


Tyr Thr Lys Tyr Asn Asp Asp Asp Thr Phe Thr Val Lys Val Gly Asp 
    50                  55                  60                  


Lys Glu Leu Phe Thr Asn Arg Trp Asn Leu Gln Ser Leu Leu Leu Ser 
65                  70                  75                  80  


Ala Gln Ile Thr Gly Met Thr Val Thr Ile Lys Thr Asn Ala Cys His 
                85                  90                  95      


Asn Gly Gly Gly Phe Ser Glu Val Ile Phe Arg Gly Gly Ser Gly Arg 
            100                 105                 110         


Leu Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala 
        115                 120                 125             


Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu 
    130                 135                 140                 


Leu Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser 
145                 150                 155                 160 


Gly Leu Glu His His His His His His 
                165                 


<210>  15
<211>  729
<212>  DNA
<213>  ˹

<220>
<223>  tacLTBTriеĺ

<400>  15
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcagcacct      240

cagaccatta ccgaactgtg cagtgaatat cgcaataccc agatctatac cattaatgat      300

aagattctga gctacaccga aagtatggcc ggtaaacgcg aaatggttat tattaccttt      360

aagagtggcg aaacctttca ggttgaagtt ccgggtagcc agcatattga tagtcagaaa      420

aaagcaattg agcgcatgaa agataccctg cgcattacct atctgaccga aaccaaaatt      480

gataaactgt gcgtttggaa taacaaaacc ccgaatagca ttgcagcaat tagtatgaaa      540

aacggcggat ccggccgcct gctgctgcgt ctggaagaac tggaacgtcg cctggaagag      600

ctggcaaagt tcgtggcagc atggaccctg aaagcggctg cggttgacct ggaactggct      660

gctctgcgtc gtcgtctgga ggagctggct cgtggcggtt ctggtctcga gcaccaccac      720

caccaccac                                                              729


<210>  16
<211>  184
<212>  PRT
<213>  ˹

<220>
<223>  LTBTri׵İ

<400>  16

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Pro Gln Thr Ile Thr Glu Leu Cys Ser Glu Tyr Arg 
            20                  25                  30          


Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr Thr Glu 
        35                  40                  45              


Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys Ser Gly 
    50                  55                  60                  


Glu Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln 
65                  70                  75                  80  


Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr Tyr Leu 
                85                  90                  95      


Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys Thr Pro 
            100                 105                 110         


Asn Ser Ile Ala Ala Ile Ser Met Lys Asn Gly Gly Ser Gly Arg Leu 
        115                 120                 125             


Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala Lys 
    130                 135                 140                 


Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu Leu 
145                 150                 155                 160 


Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser Gly 
                165                 170                 175     


Leu Glu His His His His His His 
            180                 


<210>  17
<211>  840
<212>  DNA
<213>  ˹

<220>
<223>  tacSubBTriеĺ

<400>  17
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaccatt      240

aagcgctttt tcgtgtgtgc aggcattatg ggttgtctga gcctgaatcc ggcaatggcc      300

gaatggaccg gcgatgcacg tgatggcatg tttagcggtg ttgtgattac ccagtttcat      360

accggtcaga ttgataataa gccgtatttt tgtatcgagg gcaaacagag tgccggtagt      420

agcattagtg catgtagcat gaaaaatagc agtgtgtggg gcgccagctt tagtaccctg      480

tataatcagg cactgtattt ttataccacc ggtcagccgg tgcgcatcta ttatgaaccg      540

ggcgtgtgga cctatccgcc gtttgtgaaa gcactgacca gtaatgccct ggttggcctg      600

agcacctgca ccaccagcac cgaatgtttt ggtccggatc gtaaaaagaa tagtggcgga      660

tccggccgcc tgctgctgcg tctggaagaa ctggaacgtc gcctggaaga gctggcaaag      720

ttcgtggcag catggaccct gaaagcggct gcggttgacc tggaactggc tgctctgcgt      780

cgtcgtctgg aggagctggc tcgtggcggt tctggtctcg agcaccacca ccaccaccac      840


<210>  18
<211>  221
<212>  PRT
<213>  ˹

<220>
<223>  SubBTri׵İ

<400>  18

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Thr Ile Lys Arg Phe Phe Val Cys Ala Gly Ile Met Gly 
            20                  25                  30          


Cys Leu Ser Leu Asn Pro Ala Met Ala Glu Trp Thr Gly Asp Ala Arg 
        35                  40                  45              


Asp Gly Met Phe Ser Gly Val Val Ile Thr Gln Phe His Thr Gly Gln 
    50                  55                  60                  


Ile Asp Asn Lys Pro Tyr Phe Cys Ile Glu Gly Lys Gln Ser Ala Gly 
65                  70                  75                  80  


Ser Ser Ile Ser Ala Cys Ser Met Lys Asn Ser Ser Val Trp Gly Ala 
                85                  90                  95      


Ser Phe Ser Thr Leu Tyr Asn Gln Ala Leu Tyr Phe Tyr Thr Thr Gly 
            100                 105                 110         


Gln Pro Val Arg Ile Tyr Tyr Glu Pro Gly Val Trp Thr Tyr Pro Pro 
        115                 120                 125             


Phe Val Lys Ala Leu Thr Ser Asn Ala Leu Val Gly Leu Ser Thr Cys 
    130                 135                 140                 


Thr Thr Ser Thr Glu Cys Phe Gly Pro Asp Arg Lys Lys Asn Ser Gly 
145                 150                 155                 160 


Gly Ser Gly Arg Leu Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu 
                165                 170                 175     


Glu Glu Leu Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala 
            180                 185                 190         


Val Asp Leu Glu Leu Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala 
        195                 200                 205             


Arg Gly Gly Ser Gly Leu Glu His His His His His His 
    210                 215                 220     


<210>  19
<211>  604
<212>  PRT
<213>  ˹

<220>
<223>  PglLİ

<400>  19

Met Pro Ala Glu Thr Thr Val Ser Gly Ala His Pro Ala Ala Lys Leu 
1               5                   10                  15      


Pro Ile Tyr Ile Leu Pro Cys Phe Leu Trp Ile Gly Ile Val Pro Phe 
            20                  25                  30          


Thr Phe Ala Leu Lys Leu Lys Pro Ser Pro Asp Phe Tyr His Asp Ala 
        35                  40                  45              


Ala Ala Ala Ala Gly Leu Ile Val Leu Leu Phe Leu Thr Ala Gly Lys 
    50                  55                  60                  


Lys Leu Phe Asp Val Lys Ile Pro Ala Ile Ser Phe Leu Leu Phe Ala 
65                  70                  75                  80  


Met Ala Ala Phe Trp Tyr Leu Gln Ala Arg Leu Met Asn Leu Ile Tyr 
                85                  90                  95      


Pro Gly Met Asn Asp Ile Val Ser Trp Ile Phe Ile Leu Leu Ala Val 
            100                 105                 110         


Ser Ala Trp Ala Cys Arg Ser Leu Val Ala His Phe Gly Gln Glu Arg 
        115                 120                 125             


Ile Val Thr Leu Phe Ala Trp Ser Leu Leu Ile Gly Ser Leu Leu Gln 
    130                 135                 140                 


Ser Cys Ile Val Val Ile Gln Phe Ala Gly Trp Glu Asp Thr Pro Leu 
145                 150                 155                 160 


Phe Gln Asn Ile Ile Val Tyr Ser Gly Gln Gly Val Ile Gly His Ile 
                165                 170                 175     


Gly Gln Arg Asn Asn Leu Gly His Tyr Leu Met Trp Gly Ile Leu Ala 
            180                 185                 190         


Ala Ala Tyr Leu Asn Gly Gln Arg Lys Ile Pro Ala Ala Leu Gly Val 
        195                 200                 205             


Ile Cys Leu Ile Met Gln Thr Ala Val Leu Gly Leu Val Asn Ser Arg 
    210                 215                 220                 


Thr Ile Leu Thr Tyr Ile Ala Ala Ile Ala Leu Ile Leu Pro Phe Trp 
225                 230                 235                 240 


Tyr Phe Arg Ser Asp Lys Ser Asn Arg Arg Thr Met Leu Gly Ile Ala 
                245                 250                 255     


Ala Ala Val Phe Leu Thr Ala Leu Phe Gln Phe Ser Met Asn Thr Ile 
            260                 265                 270         


Leu Glu Thr Phe Thr Gly Ile Arg Tyr Glu Thr Ala Val Glu Arg Val 
        275                 280                 285             


Ala Asn Gly Gly Phe Thr Asp Leu Pro Arg Gln Ile Glu Trp Asn Lys 
    290                 295                 300                 


Ala Leu Ala Ala Phe Gln Ser Ala Pro Ile Phe Gly His Gly Trp Asn 
305                 310                 315                 320 


Ser Phe Ala Gln Gln Thr Phe Leu Ile Asn Ala Glu Gln His Asn Ile 
                325                 330                 335     


Tyr Asp Asn Leu Leu Ser Asn Leu Phe Thr His Ser His Asn Ile Val 
            340                 345                 350         


Leu Gln Leu Leu Ala Glu Met Gly Ile Ser Gly Thr Leu Leu Val Ala 
        355                 360                 365             


Ala Thr Leu Leu Thr Gly Ile Ala Gly Leu Leu Lys Arg Pro Leu Thr 
    370                 375                 380                 


Pro Ala Ser Leu Phe Leu Ile Cys Thr Leu Ala Val Ser Met Cys His 
385                 390                 395                 400 


Ser Met Leu Glu Tyr Pro Leu Trp Tyr Val Tyr Phe Leu Ile Pro Phe 
                405                 410                 415     


Gly Leu Met Leu Phe Leu Ser Pro Ala Glu Ala Ser Asp Gly Ile Ala 
            420                 425                 430         


Phe Lys Lys Ala Ala Asn Leu Gly Ile Leu Thr Ala Ser Ala Ala Ile 
        435                 440                 445             


Phe Ala Gly Leu Leu His Leu Asp Trp Thr Tyr Thr Arg Leu Val Asn 
    450                 455                 460                 


Ala Phe Ser Pro Ala Thr Asp Asp Ser Ala Lys Thr Leu Asn Arg Lys 
465                 470                 475                 480 


Ile Asn Glu Leu Arg Tyr Ile Ser Ala Asn Ser Pro Met Leu Ser Phe 
                485                 490                 495     


Tyr Ala Asp Phe Ser Leu Val Asn Phe Ala Leu Pro Glu Tyr Pro Glu 
            500                 505                 510         


Thr Gln Thr Trp Ala Glu Glu Ala Thr Leu Lys Ser Leu Lys Tyr Arg 
        515                 520                 525             


Pro His Ser Ala Thr Tyr Arg Ile Ala Leu Tyr Leu Met Arg Gln Gly 
    530                 535                 540                 


Lys Val Ala Glu Ala Lys Gln Trp Met Arg Ala Thr Gln Ser Tyr Tyr 
545                 550                 555                 560 


Pro Tyr Leu Met Pro Arg Tyr Ala Asp Glu Ile Arg Lys Leu Pro Val 
                565                 570                 575     


Trp Ala Pro Leu Leu Pro Glu Leu Leu Lys Asp Cys Lys Ala Phe Ala 
            580                 585                 590         


Ala Ala Pro Gly His Pro Glu Ala Lys Pro Cys Lys 
        595                 600                 


<210>  20
<211>  2491
<212>  DNA
<213>  ˹

<220>
<223>  PglLеĺ

<400>  20
tctagaactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg       60

cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc      120

ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattca      180

tgcccgctga aacgaccgta tccggcgcgc accccgccgc caaactgccg atttacatcc      240

tgccctgctt cctttggata ggcatcgtcc cctttacctt cgcgctcaaa ctgaaaccgt      300

cgcccgactt ttaccacgat gccgccgccg cagccggcct gattgtcctg ttgttcctca      360

cggcaggaaa aaaactgttt gatgtcaaaa tccccgccat cagcttcctt ctgtttgcaa      420

tggcggcgtt ttggtatctt caggcacgcc tgatgaacct gatttacccc ggtatgaacg      480

acatcgtctc ttggattttc atcttgctcg ccgtcagcgc gtgggcctgc cggagcttgg      540

tcgcacactt cggacaagaa cgcatcgtga ccctgtttgc ctggtcgctg cttatcggct      600

ccctgcttca atcctgcatc gtcgtcatcc agtttgccgg ctgggaagac acccctctgt      660

ttcaaaacat catcgtttac agcgggcaag gcgtaatcgg acacatcggg cagcgcaaca      720

acctcggaca ctacctcatg tggggcatac tcgccgccgc ctacctcaac ggacaacgaa      780

aaatccccgc cgccctcggc gtaatctgcc tgattatgca gaccgccgtt ttaggtttgg      840

tcaactcgcg caccatcttg acctacatag ccgccatcgc cctcatcctt cccttctggt      900

atttccgttc ggacaaatcc aacaggcgga cgatgctcgg catagccgca gccgtattcc      960

ttaccgcgct gttccaattt tccatgaaca ccattctgga aacctttact ggcatccgct     1020

acgaaactgc cgtcgaacgc gtcgccaacg gcggtttcac agacttgccg cgccaaatcg     1080

aatggaataa agcccttgcc gccttccagt ccgccccgat attcgggcac ggctggaaca     1140

gttttgccca acaaaccttc ctcatcaatg ccgaacagca caacatatac gacaacctcc     1200

tcagcaactt gttcacccat tcccacaaca tcgtcctcca actccttgca gagatgggaa     1260

tcagcggcac gcttctggtt gccgcaaccc tgctgacggg cattgccggg ctgcttaaac     1320

gccccctgac ccccgcatcg cttttcctaa tctgcacgct tgccgtcagt atgtgccaca     1380

gtatgctcga atatcctttg tggtatgtct atttcctcat ccctttcgga ctgatgctct     1440

tcctgtcccc cgcagaggct tcagacggca tcgccttcaa aaaagccgcc aatctcggca     1500

tactgaccgc ctccgccgcc atattcgcag gattgctgca cttggactgg acatacaccc     1560

ggctggttaa cgccttttcc cccgccactg acgacagtgc caaaaccctc aaccggaaaa     1620

tcaacgagtt gcgctatatt tccgcaaaca gtccgatgct gtccttttat gccgacttct     1680

ccctcgtaaa cttcgccctg ccggaatacc ccgaaaccca gacttgggcg gaagaagcaa     1740

ccctcaaatc actaaaatac cgcccccact ccgccaccta ccgcatcgcc ctctacctga     1800

tgcggcaagg caaagttgca gaagcaaaac aatggatgcg ggcgacacag tcctattacc     1860

cctacctgat gccccgatac gccgacgaaa tccgcaaact gcccgtatgg gcgccgctgc     1920

tacccgaact gctcaaagac tgcaaagcct tcgccgccgc gcccggtcat ccggaagcaa     1980

aaccctgcaa atgaaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac     2040

agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg     2100

cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta     2160

gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct     2220

cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt     2280

aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg     2340

gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat     2400

ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta     2460

tccgctcatg agacgagctc ggccgctcga g                                    2491


<210>  21
<211>  213
<212>  PRT
<213>  ˹

<220>
<223>  CTBTri4573İ

<400>  21

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His 
            20                  25                  30          


Asn Thr Gln Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu 
        35                  40                  45              


Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly 
    50                  55                  60                  


Ala Ile Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln 
65                  70                  75                  80  


Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu 
                85                  90                  95      


Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro 
            100                 105                 110         


His Ala Ile Ala Ala Ile Ser Met Ala Asn Gly Gly Ser Gly Arg Leu 
        115                 120                 125             


Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala Lys 
    130                 135                 140                 


Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu Leu 
145                 150                 155                 160 


Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser Gly 
                165                 170                 175     


Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly Asn 
            180                 185                 190         


Asn Thr Ser Ala Gly Val Ala Thr Ser Ser Glu Ile Lys Leu Glu His 
        195                 200                 205             


His His His His His 
    210             


<210>  22
<211>  816
<212>  DNA
<213>  ˹

<220>
<223>  CTBTri4573еĺ

<400>  22
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaccccg      240

cagaacatca ccgacctgtg cgccgagtac cacaacaccc aaatttatac cctgaacgac      300

aaaattttta gctacaccga gagcctggca ggcaagcgcg agatggccat catcaccttc      360

aagaacggcg ccattttcca ggtggaggtg ccgggcagcc agcacatcga cagtcagaag      420

aaggccatcg agcgcatgaa ggacaccctg cgcatcgcct acctgaccga ggccaaggtg      480

gagaagctgt gcgtgtggaa caacaagacc ccgcacgcca tcgccgcaat cagcatggcc      540

aacggcggat ccggccgcct gctgctgcgt ctggaagaac tggaacgtcg cctggaagag      600

ctggcaaagt tcgtggcagc atggaccctg aaagcggctg cggttgacct ggaactggct      660

gctctgcgtc gtcgtctgga ggagctggct cgtggcggtt ctggtagcgc cgtgaccgag      720

tactatctga accatggcga gtggccgggt aataacacca gcgccggcgt ggccacaagc      780

agtgagatca agctcgagca ccaccaccac caccac                                816


<210>  23
<211>  198
<212>  PRT
<213>  ˹

<220>
<223>  StxBTri4573İ

<400>  23

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Lys Lys Thr Leu Leu Ile Ala Ala Ser Leu Ser Phe Phe 
            20                  25                  30          


Ser Ala Ser Ala Leu Ala Thr Pro Asp Cys Val Thr Gly Lys Val Glu 
        35                  40                  45              


Tyr Thr Lys Tyr Asn Asp Asp Asp Thr Phe Thr Val Lys Val Gly Asp 
    50                  55                  60                  


Lys Glu Leu Phe Thr Asn Arg Trp Asn Leu Gln Ser Leu Leu Leu Ser 
65                  70                  75                  80  


Ala Gln Ile Thr Gly Met Thr Val Thr Ile Lys Thr Asn Ala Cys His 
                85                  90                  95      


Asn Gly Gly Gly Phe Ser Glu Val Ile Phe Arg Gly Gly Ser Gly Arg 
            100                 105                 110         


Leu Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala 
        115                 120                 125             


Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu 
    130                 135                 140                 


Leu Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser 
145                 150                 155                 160 


Gly Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly 
                165                 170                 175     


Asn Asn Thr Ser Ala Gly Val Ala Thr Ser Ser Glu Ile Lys Leu Glu 
            180                 185                 190         


His His His His His His 
        195             


<210>  24
<211>  771
<212>  DNA
<213>  ˹

<220>
<223>  StxBTri4573еĺ

<400>  24
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaaaaaa      240

acattattaa tagctgcatc gctttcattt ttttcagcaa gtgcgctggc gacgcctgat      300

tgtgtaactg gaaaggtgga gtatacaaaa tataatgatg acgatacctt tacagttaaa      360

gtgggtgata aagaattatt taccaacaga tggaatcttc agtctcttct tctcagtgcg      420

caaattacgg ggatgactgt aaccattaaa actaatgcct gtcataatgg agggggattc      480

agcgaagtta tttttcgtgg cggatccggc cgcctgctgc tgcgtctgga agaactggaa      540

cgtcgcctgg aagagctggc aaagttcgtg gcagcatgga ccctgaaagc ggctgcggtt      600

gacctggaac tggctgctct gcgtcgtcgt ctggaggagc tggctcgtgg cggttctggt      660

agcgccgtga ccgagtacta tctgaaccat ggcgagtggc cgggtaataa caccagcgcc      720

ggcgtggcca caagcagtga gatcaagctc gagcaccacc accaccacca c               771


<210>  25
<211>  213
<212>  PRT
<213>  ˹

<220>
<223>  LTBTri4573İ

<400>  25

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Pro Gln Thr Ile Thr Glu Leu Cys Ser Glu Tyr Arg 
            20                  25                  30          


Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr Thr Glu 
        35                  40                  45              


Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys Ser Gly 
    50                  55                  60                  


Glu Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln 
65                  70                  75                  80  


Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr Tyr Leu 
                85                  90                  95      


Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys Thr Pro 
            100                 105                 110         


Asn Ser Ile Ala Ala Ile Ser Met Lys Asn Gly Gly Ser Gly Arg Leu 
        115                 120                 125             


Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu Glu Glu Leu Ala Lys 
    130                 135                 140                 


Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Val Asp Leu Glu Leu 
145                 150                 155                 160 


Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala Arg Gly Gly Ser Gly 
                165                 170                 175     


Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly Asn 
            180                 185                 190         


Asn Thr Ser Ala Gly Val Ala Thr Ser Ser Glu Ile Lys Leu Glu His 
        195                 200                 205             


His His His His His 
    210             


<210>  26
<211>  816
<212>  DNA
<213>  ˹

<220>
<223>  LTBTri4573еĺ

<400>  26
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcagcacct      240

cagaccatta ccgaactgtg cagtgaatat cgcaataccc agatctatac cattaatgat      300

aagattctga gctacaccga aagtatggcc ggtaaacgcg aaatggttat tattaccttt      360

aagagtggcg aaacctttca ggttgaagtt ccgggtagcc agcatattga tagtcagaaa      420

aaagcaattg agcgcatgaa agataccctg cgcattacct atctgaccga aaccaaaatt      480

gataaactgt gcgtttggaa taacaaaacc ccgaatagca ttgcagcaat tagtatgaaa      540

aacggcggat ccggccgcct gctgctgcgt ctggaagaac tggaacgtcg cctggaagag      600

ctggcaaagt tcgtggcagc atggaccctg aaagcggctg cggttgacct ggaactggct      660

gctctgcgtc gtcgtctgga ggagctggct cgtggcggtt ctggtagcgc cgtgaccgag      720

tactatctga accatggcga gtggccgggt aataacacca gcgccggcgt ggccacaagc      780

agtgagatca agctcgagca ccaccaccac caccac                                816


<210>  27
<211>  250
<212>  PRT
<213>  ˹

<220>
<223>  SubBTri4573İ

<400>  27

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Thr Ile Lys Arg Phe Phe Val Cys Ala Gly Ile Met Gly 
            20                  25                  30          


Cys Leu Ser Leu Asn Pro Ala Met Ala Glu Trp Thr Gly Asp Ala Arg 
        35                  40                  45              


Asp Gly Met Phe Ser Gly Val Val Ile Thr Gln Phe His Thr Gly Gln 
    50                  55                  60                  


Ile Asp Asn Lys Pro Tyr Phe Cys Ile Glu Gly Lys Gln Ser Ala Gly 
65                  70                  75                  80  


Ser Ser Ile Ser Ala Cys Ser Met Lys Asn Ser Ser Val Trp Gly Ala 
                85                  90                  95      


Ser Phe Ser Thr Leu Tyr Asn Gln Ala Leu Tyr Phe Tyr Thr Thr Gly 
            100                 105                 110         


Gln Pro Val Arg Ile Tyr Tyr Glu Pro Gly Val Trp Thr Tyr Pro Pro 
        115                 120                 125             


Phe Val Lys Ala Leu Thr Ser Asn Ala Leu Val Gly Leu Ser Thr Cys 
    130                 135                 140                 


Thr Thr Ser Thr Glu Cys Phe Gly Pro Asp Arg Lys Lys Asn Ser Gly 
145                 150                 155                 160 


Gly Ser Gly Arg Leu Leu Leu Arg Leu Glu Glu Leu Glu Arg Arg Leu 
                165                 170                 175     


Glu Glu Leu Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala 
            180                 185                 190         


Val Asp Leu Glu Leu Ala Ala Leu Arg Arg Arg Leu Glu Glu Leu Ala 
        195                 200                 205             


Arg Gly Gly Ser Gly Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly 
    210                 215                 220                 


Glu Trp Pro Gly Asn Asn Thr Ser Ala Gly Val Ala Thr Ser Ser Glu 
225                 230                 235                 240 


Ile Lys Leu Glu His His His His His His 
                245                 250 


<210>  28
<211>  927
<212>  DNA
<213>  ˹

<220>
<223>  SubBTri4573еĺ

<400>  28
tctagatgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg       60

ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg      120

ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcatg      180

aagaaaattt ggctggcctt agccggcctg gttctggcat tcagcgccag cgcaaccatt      240

aagcgctttt tcgtgtgtgc aggcattatg ggttgtctga gcctgaatcc ggcaatggcc      300

gaatggaccg gcgatgcacg tgatggcatg tttagcggtg ttgtgattac ccagtttcat      360

accggtcaga ttgataataa gccgtatttt tgtatcgagg gcaaacagag tgccggtagt      420

agcattagtg catgtagcat gaaaaatagc agtgtgtggg gcgccagctt tagtaccctg      480

tataatcagg cactgtattt ttataccacc ggtcagccgg tgcgcatcta ttatgaaccg      540

ggcgtgtgga cctatccgcc gtttgtgaaa gcactgacca gtaatgccct ggttggcctg      600

agcacctgca ccaccagcac cgaatgtttt ggtccggatc gtaaaaagaa tagtggcgga      660

tccggccgcc tgctgctgcg tctggaagaa ctggaacgtc gcctggaaga gctggcaaag      720

ttcgtggcag catggaccct gaaagcggct gcggttgacc tggaactggc tgctctgcgt      780

cgtcgtctgg aggagctggc tcgtggcggt tctggtagcg ccgtgaccga gtactatctg      840

aaccatggcg agtggccggg taataacacc agcgccggcg tggccacaag cagtgagatc      900

aagctcgagc accaccacca ccaccac                                          927


<210>  29
<211>  29
<212>  PRT
<213>  ˹

<220>
<223>  ǻУPilE S45~K73

<400>  29

Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly Asn 
1               5                   10                  15      


Asn Thr Ser Ala Gly Val Ala Thr Ser Ser Glu Ile Lys 
            20                  25                  


<210>  30
<211>  4
<212>  PRT
<213>  ˹

<220>
<223>  Linker

<400>  30

Gly Gly Ser Gly 
1               


<210>  31
<211>  19
<212>  PRT
<213>  ˹

<220>
<223>  DsbAź

<400>  31

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210>  32
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  SacI-UP

<400>  32
cgagctctgc ataattcgtg tcgctcaagg cgcactc                                37


<210>  33
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  XhoI-Down

<400>  33
ccgctcgagc ttgatctctc actgcttgtg gccacg                                 36


