                         SEQUENCE LISTING

<110>  GE Healthcare Bioprocess R&D AB
 
<120>  METHOD OF SEPARATION

<130>  502312-WO-2

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand consensus sequence


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Ala Gln Xaa Ala Phe Tyr Glu Ile Leu Xaa Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Xaa Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Xaa Ala Gln 
        35                  40                  45          


<210>  2
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand example

<400>  2

Ala Gln Glu Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        35                  40                  45          


<210>  3
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand example

<400>  3

Ala Gln Lys Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        35                  40                  45          


<210>  4
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand example

<400>  4

Ala Gln Glu Ala Phe Tyr Glu Ile Leu Lys Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        35                  40                  45          


<210>  5
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand example

<400>  5

Ala Gln Glu Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Ala Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        35                  40                  45          


<210>  6
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand example

<400>  6

Ala Gln Glu Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
1               5                   10                  15      


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
            20                  25                  30          


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Trp Ala Gln 
        35                  40                  45          


<210>  7
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hexameric ligand

<400>  7

Ala Gln Gly Thr Val Asp Ala Lys Phe Asp Lys Glu Ala Gln Glu Ala 
1               5                   10                  15      


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
            20                  25                  30          


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Ala Ile 
        35                  40                  45              


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp 
    50                  55                  60                  


Ala Lys Phe Asp Lys Glu Ala Gln Glu Ala Phe Tyr Glu Ile Leu His 
65                  70                  75                  80  


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu 
                85                  90                  95      


Lys Asp Asp Pro Ser Val Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys 
            100                 105                 110         


Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Ala Lys Phe Asp Lys Glu 
        115                 120                 125             


Ala Gln Glu Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
    130                 135                 140                 


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val 
145                 150                 155                 160 


Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
                165                 170                 175     


Pro Lys Val Asp Ala Lys Phe Asp Lys Glu Ala Gln Glu Ala Phe Tyr 
            180                 185                 190         


Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe 
        195                 200                 205             


Ile Gln Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Ala Ile Leu Ala 
    210                 215                 220                 


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Ala Lys 
225                 230                 235                 240 


Phe Asp Lys Glu Ala Gln Glu Ala Phe Tyr Glu Ile Leu His Leu Pro 
                245                 250                 255     


Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp 
            260                 265                 270         


Asp Pro Ser Val Ser Lys Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn 
        275                 280                 285             


Asp Ala Gln Ala Pro Lys Val Asp Ala Lys Phe Asp Lys Glu Ala Gln 
    290                 295                 300                 


Glu Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln 
305                 310                 315                 320 


Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val Ser Lys 
                325                 330                 335     


Ala Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
            340                 345                 350         


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  8

Ala Pro Lys Val Asp Ala Lys Phe Asp Lys Glu 
1               5                   10      


<210>  9
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  9

Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu 
1               5                   10      


<210>  10
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  10

Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys Glu 
1               5                   10      


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  11

Ala Pro Lys Val Phe Asp Lys Glu 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  12

Ala Pro Ala Lys Phe Asp Lys Glu 
1               5               


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  13

Ala Lys Phe Asp Lys Glu 
1               5       


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  14

Ala Pro Lys Val Asp Ala 
1               5       


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  15

Val Asp Ala Lys Phe Asp Lys Glu 
1               5               


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  16

Ala Pro Lys Lys Phe Asp Lys Glu 
1               5               


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  17

Ala Pro Lys Tyr Glu Asp Gly Val Asp Ala Lys Phe Asp Lys Glu 
1               5                   10                  15  


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  18

Tyr Glu Asp Gly 
1               


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader

<400>  19

Ala Gln Gly Thr 
1               


<210>  20
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader

<400>  20

Val Asp Ala Lys Phe Asp Lys Glu 
1               5               


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader

<400>  21

Ala Gln Val Asp Ala Lys Phe Asp Lys Glu 
1               5                   10  


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader

<400>  22

Ala Gln Gly Thr Val Asp Ala Lys Phe Asp Lys Glu 
1               5                   10          


<210>  23
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH

<400>  23

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Arg Trp Ser Gly Gly Tyr Thr Tyr Tyr Thr Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu Pro Gln 
            100                 105                 110         


Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
        115                 120                 125             


Ser Ser Leu Glu His His His His His His 
    130                 135             


<210>  24
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1

<400>  24

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Thr Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  25
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (T57K)

<400>  25

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Lys Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  26
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (T57K,K64R)

<400>  26

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Lys Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  27
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (T57P)

<400>  27

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Pro Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  28
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (T57S)

<400>  28

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Ser Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  29
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (T57R)

<400>  29

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Arg Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  30
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-EgA1 (S70T)

<400>  30

Ala Gln Val Asp Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Arg Trp Ser Gly Gly Tyr Thr Tyr Tyr Thr Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Thr Arg Asp Asn Ala Lys Thr Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Thr Tyr Leu Ser Ser Asp Tyr Ser Arg Tyr Ala Leu 
            100                 105                 110         


Pro Gln Arg Pro Leu Asp Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             


Thr Val Ser Ser Leu Glu His His His His His His 
    130                 135                 140 


<210>  31
<211>  286
<212>  PRT
<213>  Staphylococcus aureus

<400>  31

Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn Met Pro Asn Leu Asn 
1               5                   10                  15      


Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 
            20                  25                  30          


Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys Leu Asn Asp Ser Gln 
        35                  40                  45              


Ala Pro Lys Ala Asp Ala Gln Gln Asn Lys Phe Asn Lys Asp Gln Gln 
    50                  55                  60                  


Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln 
65                  70                  75                  80  


Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Thr 
                85                  90                  95      


Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
            100                 105                 110         


Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
        115                 120                 125             


Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
    130                 135                 140                 


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
145                 150                 155                 160 


Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn 
                165                 170                 175     


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
            180                 185                 190         


Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro 
        195                 200                 205             


Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
    210                 215                 220                 


Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
225                 230                 235                 240 


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
                245                 250                 255     


Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile 
            260                 265                 270         


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
        275                 280                 285     


