                         SEQUENCE LISTING

<110>  Dharmacon, Inc.
 
<120>  Multiplex shRNA for use in Vectors

<130>  Dharma 0103-PROV

<150>  62/842,049
<151>  2019-05-02

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1
gaatccaact tacttcttca cgacaacact tacctttgtg caagcactta tgatcctgat       60


<210>  2
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2
gagtccaatt tacttcttca cacattcctg tatttgcaag cacttgtcct ga               52


<210>  3
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
gctctttact cctcgcctat tgagaccttt aggatgcgga aactgcttta cttaacatct       60


<210>  4
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4
tgttggatgt ggacacttct ttactaatac cttgttaact ggtcttaata cg               52


<210>  5
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
cttcgatacg atacagcggt atggtgcacc tcaaatcctg tggtc                       45


<210>  6
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
tcgggcactt caattccgta gttatcttga accttcgtgg agcgacagat acatct           56


<210>  7
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
tcctcgtaca tatagcatga ctagctttgt gacctttgac tttctctatc aatctat          57


<210>  8
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
acatgaggat atcaggtagt ctgcaacact ctaagggcgg ttatctgtgg t                51


<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
atccaactta cgtatccaat atcggtcttg ctaatcatgt gtcctgttac                  50


<210>  10
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
ttcgatcgat acgatagcgg ggagttcgcg ttgttccgac gttgtacact t                51


<210>  11
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
tgctaacgtc ggtgatcgcg cgaaatagtc tagggccgac cgggtcgc                    48


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
tagagtacga gtcggcgaac agttacatcg aagtcgcgtc                             40


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
tcaccacaac acattccttt attgtagcaa                                        30


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
ctgagctatg ccacctggga tgccttcacg                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
cctgttaact ggtcttaata cggtgtctcc                                        30


<210>  16
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
tcgggcactt caattccgta gttatcttga accttcgtgg agcgacagat acatgc           56


<210>  17
<211>  150
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
tcagacccct tacccaccca gcaacccaaa gtctactctc tagtccttag ggaggttgtg       60

ggggcggaaa gggggacggg gctgaatttc ttccttcccc aacccccttc ccttctcctc      120

cagatagatg caaagctgaa tctcccgccc                                       150


<210>  18
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
gcggaaaggg ggacggggct gaatttcttc cttccccaac ccccttccct tctcctccag       60

atagatgcaa agctgaatct cccgccc                                           87


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19
ccaatcgagc tctcccactc ctaccctccc cagtggg                                37


<210>  20
<211>  150
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20
ccaatcgagc tctcccactc ctaccctccc cagtgggact gccccactgc cccctcccag       60

atagggcaaa gtgggtgcag accaaggagg acaagctgtg agtggggttg cagaacaagt      120

ctggagaacc ctgctttatg ccgtcctcta                                       150


<210>  21
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21
ccaatcgagc tctcccactc ctaccctccc cagtgggact gccccactgc cccctcccag       60

atagggcaaa gtgggtgcag a                                                 81


<210>  22
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22
ccaatcgagc tctcccactc ctaccctccc cagtggga                               38


<210>  23
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
ttccgaagag accaaagatg ggattgagtt ttgaactcgt ctttgctctc tttggag          57


<210>  24
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  24
tatcagccat gctaattcgg ggattgagtt ttgaactctg aattaccatg gctgatg          57


<210>  25
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  25
tccccaaaga ggacatccgg ggattgagtt ttgaactccg gatgtgctct ttggggg          57


<210>  26
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  26
ttaaattaaa acccgcaggg ggattgagtt ttgaactccc tgcggctttt aatttag          57


<210>  27
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27
tacttgacag ccttcaggtg ggattgagtt ttgaactcac ctgaacgctg tcaagtg          57


<210>  28
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28
ttcgaatcaa tccaacagtg ggattgagtt ttgaactcac tgttgcattg attcgag          57


<210>  29
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29
tatattctcc aggaaatgcg ggattgagtt ttgaactcgc atttcgtgga gaatatg          57


<210>  30
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30
taaaactcac actggttgcg ggattgagtt ttgaactcgc aaccactgtg agttttg          57


<210>  31
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (25)..()
<223>  n is a mature strand insert in an miRNA cassette

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (51)..()
<223>  n is a mature strand insert in an miRNA cassette

<400>  31
ugcucgcuca gcugaucugu ggcungauug aguuuugaac naguuacauc agucgguuuu       60

cgucgagggc                                                              70


<210>  32
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (25)..(43)
<223>  n is any of A, C, G, or U

<220>
<221>  misc_feature
<222>  (59)..(77)
<223>  n is any of A, C, G, or U

<400>  32
ugcucgcuca gcugaucugu ggcunnnnnn nnnnnnnnnn nnngauugag uuuugaacnn       60

nnnnnnnnnn nnnnnnnagu uacaucaguc gguuuucguc gagggc                     106


<210>  33
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33
tccaggttaa tgaaagccgg ggattgagtt ttgaactccg gctttgatta acctggg          57


<210>  34
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34
taaaccgaac ttctcctgag ggattgagtt ttgaactctc aggagtagtt cggtttg          57


<210>  35
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  35
tcgacgatta actacttctg ggattgagtt ttgaactcag aagtacttaa tcgtcgg          57


<210>  36
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  36
tatgggttca caaatcagcg ggattgagtt ttgaactcgt tgattagtga actcatg          57


<210>  37
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  37
tgtcgggttc gaatcaatcc aacagttgac tgttgaatct catggcactg ttgcattgat       60

tcgagtctga ca                                                           72


<210>  38
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  38
gcgactatat tctccaggaa atgcaagctg tgaagccaca gatgggcgca tttcgtggag       60

aatatagctg c                                                            71


<210>  39
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  39
ctgtaaaact cacactggtt gcgggttttt gcctccaact gacgcaacca ctgtgagttt       60

tacag                                                                   65


<210>  40
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  40
ctgttcgaat caatccaaca gtgggttttt gcctccaact gacactgttg cattgattcg       60

aacag                                                                   65


<210>  41
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  41
tgtcgggtat attctccagg aaatgctgac tgttgaatct catggcgcat ttcgtggaga       60

atatgtctga ca                                                           72


<210>  42
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  42
gcgactaaaa ctcacactgg ttgcaagctg tgaagccaca gatgggcgca accactgtga       60

gttttagctg c                                                            71


<210>  43
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  43
gcgacttcga atcaatccaa cagtaagctg tgaagccaca gatgggcact gttgcattga       60

ttcgaagctg c                                                            71


<210>  44
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  44
tgtcgggtaa aactcacact ggttgctgac tgttgaatct catggcgcaa ccactgtgag       60

ttttgtctga ca                                                           72


<210>  45
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  45
ctgtatattc tccaggaaat gcgggttttt gcctccaact gacgcatttc gtggagaata       60

tacag                                                                   65


