                         SEQUENCE LISTING

<110>  H. Lee Moffitt Cancer Center and Research Institute, Inc.
 
<120>  ENGINEERING BROADLY REACTIVE HUMAN NOTCH LIGANDS AS NOVEL TOOLS 
       FOR BIOMEDICAL APPLICATIONS

<130>  10110-227WO1

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  296
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Gly Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Phe Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Ile Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp His Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Leu Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala 
            260                 265                 270         


Thr Cys Ser Asn Ser Gly Gln Arg Ser Tyr Thr Cys Thr Cys Arg Pro 
        275                 280                 285             


Gly Tyr Thr Gly Val Asp Cys Glu 
    290                 295     


<210>  2
<211>  295
<212>  PRT
<213>  Homo sapiens

<400>  2

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Asn Ser Phe Ala Val 
    50                  55                  60                  


Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro Leu 
65                  70                  75                  80  


Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp His 
                85                  90                  95      


Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala Leu 
            100                 105                 110         


Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn Trp 
        115                 120                 125             


Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser Tyr 
    130                 135                 140                 


Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg Leu 
145                 150                 155                 160 


Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro Asp 
                165                 170                 175     


Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln Gln 
            180                 185                 190         


Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser Lys 
        195                 200                 205             


Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys Asn 
    210                 215                 220                 


Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr Pro 
225                 230                 235                 240 


Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp Gln 
                245                 250                 255     


Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala Thr 
            260                 265                 270         


Cys Ser Asn Ser Gly Gln Arg Ser Tyr Thr Cys Thr Cys Arg Pro Gly 
        275                 280                 285             


Tyr Thr Gly Val Asp Cys Glu 
    290                 295 


<210>  3
<211>  295
<212>  PRT
<213>  Homo sapiens

<400>  3

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Asn Ser Phe Ala Val 
    50                  55                  60                  


Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro Leu 
65                  70                  75                  80  


Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp His 
                85                  90                  95      


Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala Leu 
            100                 105                 110         


Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn Trp 
        115                 120                 125             


Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser Tyr 
    130                 135                 140                 


Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg Leu 
145                 150                 155                 160 


Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro Asp 
                165                 170                 175     


Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln Gln 
            180                 185                 190         


Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser Lys 
        195                 200                 205             


Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys Asn 
    210                 215                 220                 


Glu Cys Ile Pro Tyr Pro Gly Cys Arg His Gly Thr Cys Ser Thr Pro 
225                 230                 235                 240 


Trp Gln Cys Leu Cys Asp Glu Gly Trp Gly Gly Leu Tyr Cys Asp Gln 
                245                 250                 255     


Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala Thr 
            260                 265                 270         


Cys His Asn Ser Gly Pro Arg Ser Tyr Thr Cys Thr Cys Arg Pro Gly 
        275                 280                 285             


Tyr Thr Gly Val Asp Cys Glu 
    290                 295 


<210>  4
<211>  295
<212>  PRT
<213>  Homo sapiens

<400>  4

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Asn Ser Phe Ala Val 
    50                  55                  60                  


Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro Leu 
65                  70                  75                  80  


Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp His 
                85                  90                  95      


Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala Leu 
            100                 105                 110         


Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn Trp 
        115                 120                 125             


Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser Tyr 
    130                 135                 140                 


Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg Leu 
145                 150                 155                 160 


Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro Asp 
                165                 170                 175     


Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln Gln 
            180                 185                 190         


Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser Lys 
        195                 200                 205             


Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys Asn 
    210                 215                 220                 


Glu Cys Ile Pro His Pro Gly Cys Arg His Gly Thr Cys Ser Thr Pro 
225                 230                 235                 240 


Trp Gln Cys Leu Cys Asp Glu Gly Trp Gly Gly Leu Tyr Cys Asp Gln 
                245                 250                 255     


Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala Thr 
            260                 265                 270         


Cys Arg Asn Ser Gly Pro Arg Ser Tyr Thr Cys Thr Cys Arg Pro Gly 
        275                 280                 285             


Tyr Thr Gly Val Asp Cys Glu 
    290                 295 


