                         SEQUENCE LISTING

<110>  Board of Regents, The University of Texas System
 
<120>  TARGETING OTUB1 IN IMMUNOTHERAPY

<130>  UTFC.P1462WO

<140>  not yet known
<141>  2020-05-07

<150>  US 62/844,217
<151>  2019-05-07

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  1
uccgacuacc uuguggucu                                                    19


<210>  2
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  2
aaggaguugc agcgguuca                                                    19


<210>  3
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  3
cuguuucuau cgggcuuuc                                                    19


<210>  4
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  4
gcuuucggau ucucccacu                                                    19


<210>  5
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  5
gcugugucug ccaagagca                                                    19


<210>  6
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  6
cacguucaug gaccugauu                                                    19


<210>  7
<211>  2016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence encoding a CAR polypeptide

<400>  7
atggctttgc cagtgacagc tcttctcctt ccactggccc tcctccttca cgccgctagg       60

ccagagcaga aacttatttc agaggaagac ctggacattc aaatgacaca aactacttct      120

tctctctccg cctcacttgg tgaccgcgtc actattagtt gccgcgctag tcaagatatt      180

agtaagtacc tgaattggta tcaacaaaaa cctgacggga ctgtaaagct gcttatatat      240

catacttcta ggctgcattc tggagtacct tcacgattta gcggtagcgg atccggcacc      300

gactactccc tcacaattag caatctggag caagaggaca tagccaccta cttctgccag      360

caagggaata ccttgccata cactttcggt ggtggaacta agctcgaaat tactgggggt      420

ggaggcagtg gcggaggggg gtcaggtggg ggaggttcag aagtcaaact ccaggaatct      480

ggacctggac tcgttgcccc ttcccaatcc cttagtgtta catgcactgt atcaggtgta      540

tccctccctg attacggtgt ctcctggatt cggcagcctc ctcggaaggg tctcgagtgg      600

ttgggagtga tttgggggtc tgaaactact tattataaca gtgcccttaa gagtagattg      660

actataatta aggataacag taagtcacaa gtattcctca aaatgaattc cttgcaaaca      720

gacgatacag caatatatta ctgcgcaaaa cactactact atggcggtag ttacgctatg      780

gactattggg gtcaaggaac ctctgtcaca gtttctagca ttgagttcat gtatccccca      840

ccttacttgg acaatgaaag gtctaatggg accatcatac acattaaaga gaaacacctg      900

tgtcatactc agagttctcc aaaattgttc tgggccttgg ttgtcgttgc cggcgtactg      960

ttctgttacg gtctcttggt taccgtggca ctttgtgtta tctggactaa ttcccggcgg     1020

aatcggggtg gacagagcga ttacatgaat atgaccccaa gaagacctgg actgaccagg     1080

aaaccatatc aaccctatgc tcctgctcgg gactttgctg cttaccgccc acgcgcaaag     1140

ttttctagga gcgctgaaac cgctgccaac ctccaagacc ctaatcagct ttacaatgaa     1200

ttgaacttgg gacgccggga ggagtatgac gtccttgaga aaaagcgggc tcgggatcca     1260

gaaatgggcg gaaagcaaca gaggcgaaga aatccacaag agggggtcta taacgctctt     1320

cagaaagata aaatggctga ggcatatagc gaaattggga ccaaggggga gagaagaaga     1380

ggcaagggac atgacgggct ttaccagggt ttgtctaccg caacaaaaga cacctatgat     1440

gctttgcaca tgcaaacact ggctcctaga gccaccaact tctccctgct gaagcaggcc     1500

ggcgacgtgg aggagaaccc cggccccatg aacccagcca tcagcgtcgc tctcctgctc     1560

tcagtcttgc aggtgtcccg agggcagaag gtgaccagcc tgacagcctg cctggtgaac     1620

caaaaccttc gcctggactg ccgccatgag aataacacca aggataactc catccagcat     1680

gagttcagcc tgacccgaga gaagaggaag cacgtgctct caggcaccct cgggataccc     1740

gagcacacgt accgctcccg cgtcaccctc tccaaccagc cctatatcaa ggtccttacc     1800

ctagccaact tcaccaccaa ggatgagggc gactactttt gtgagcttcg agtctcgggc     1860

gcgaatccca tgagctccaa taaaagtatc agtgtgtata gagacaaact ggtcaagtgt     1920

ggcggcataa gcctgctggt tcagaacaca tcctggatgc tgctgctgct gctttccctc     1980

tccctcctcc aagccctgga cttcatttct ctgtga                               2016


<210>  8
<211>  671
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR polypeptide

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asp 
            20                  25                  30          


Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp 
        35                  40                  45              


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu 
    50                  55                  60                  


Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 
65                  70                  75                  80  


His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
                85                  90                  95      


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu 
            100                 105                 110         


Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
        115                 120                 125             


Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser 
145                 150                 155                 160 


Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr 
                165                 170                 175     


Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln 
            180                 185                 190         


Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu 
        195                 200                 205             


Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys 
    210                 215                 220                 


Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 
225                 230                 235                 240 


Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly 
                245                 250                 255     


Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
            260                 265                 270         


Ser Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg Ser 
        275                 280                 285             


Asn Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr Gln 
    290                 295                 300                 


Ser Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val Leu 
305                 310                 315                 320 


Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr 
                325                 330                 335     


Asn Ser Arg Arg Asn Arg Gly Gly Gln Ser Asp Tyr Met Asn Met Thr 
            340                 345                 350         


Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro 
        355                 360                 365             


Ala Arg Asp Phe Ala Ala Tyr Arg Pro Arg Ala Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Glu Thr Ala Ala Asn Leu Gln Asp Pro Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Glu Lys Lys Arg 
                405                 410                 415     


Ala Arg Asp Pro Glu Met Gly Gly Lys Gln Gln Arg Arg Arg Asn Pro 
            420                 425                 430         


Gln Glu Gly Val Tyr Asn Ala Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Thr Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Thr Leu Ala Pro Arg Ala Thr Asn Phe Ser Leu 
                485                 490                 495     


Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Asn Pro 
            500                 505                 510         


Ala Ile Ser Val Ala Leu Leu Leu Ser Val Leu Gln Val Ser Arg Gly 
        515                 520                 525             


Gln Lys Val Thr Ser Leu Thr Ala Cys Leu Val Asn Gln Asn Leu Arg 
    530                 535                 540                 


Leu Asp Cys Arg His Glu Asn Asn Thr Lys Asp Asn Ser Ile Gln His 
545                 550                 555                 560 


Glu Phe Ser Leu Thr Arg Glu Lys Arg Lys His Val Leu Ser Gly Thr 
                565                 570                 575     


Leu Gly Ile Pro Glu His Thr Tyr Arg Ser Arg Val Thr Leu Ser Asn 
            580                 585                 590         


Gln Pro Tyr Ile Lys Val Leu Thr Leu Ala Asn Phe Thr Thr Lys Asp 
        595                 600                 605             


Glu Gly Asp Tyr Phe Cys Glu Leu Arg Val Ser Gly Ala Asn Pro Met 
    610                 615                 620                 


Ser Ser Asn Lys Ser Ile Ser Val Tyr Arg Asp Lys Leu Val Lys Cys 
625                 630                 635                 640 


Gly Gly Ile Ser Leu Leu Val Gln Asn Thr Ser Trp Met Leu Leu Leu 
                645                 650                 655     


Leu Leu Ser Leu Ser Leu Leu Gln Ala Leu Asp Phe Ile Ser Leu 
            660                 665                 670     


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  9
cgtgaaaaga tgacccagat ca                                                22


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  10
cacagcctgg atggctacgt                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
gtagcgactc cgaaggtgtt                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
accagaggat tctgcacagc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
agcaccagcc aagccatgta                                                   20


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
cgtagggaga ggtgctgttt t                                                 21


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
gcagtcgtgt ttgtcactcg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
agagcaagca atgacaggga                                                   20


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  17
tctgtgcact gtgcatctct c                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  18
gacttggtgc atggaacact g                                                 21


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  19
tgaatgaacc ttccaagact caga                                              24


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  20
ggttatggtc gatctttagc tg                                                22


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  21
tgtcagcgtg cgacatggct                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  22
gagtgaagcg gcggctggtg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  23
ataccccctc gctctctgtt                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  24
acataggtcc ccatctgcct                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  25
gggatcatct tgctggtgaa                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
aggtccctgt catgcttctg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
agacatccgt tccccctaca                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
gcagggtgct gacataccat                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
ccatggaacc gatcagtgtg a                                                 21


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  30
ttttcatccc ggaagcaggg                                                   20


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  31
ctcaggagcc cacaacgagt gc                                                22


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  32
tctgggcttc ttgcctcttg ggt                                               23


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
cgggaagagc tctggagaac c                                                 21


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
gcattctcta acagtctgtg cc                                                22


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
gccagggaac cgcttatatg                                                   20


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
gacgatcatc tgggtcacat tgt                                               23


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  37
tcatcaccta cagcgacgag                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  38
gggtagaagg tggggatttc                                                   20


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  39
gcctcttatg cttcaaacaa ca                                                22


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  40
cagcagcatt gccaaacagt                                                   20


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  41
gaaacccctc cctcttcagg a                                                 21


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  42
agggctgcac agataaaact tc                                                22


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  43
gatcgccgga ttggaacaga                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  44
ggtctagctg cttagcgtcc                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  45
gctgtgctta tgggcttctc                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  46
cacatacatg ggcacaaagc                                                   20


