                         SEQUENCE LISTING

<110>  Case Western Reserve University
       WEISS, Michael  A.
       GLIDDEN, Michael II D.
       ISMAIL-BEIGI, Faramarz
 
<120>  Variant Single-Chain Insulin Analogues

<130>  200512-00448

<150>  62/849,363
<151>  2020-05-17

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Homo sapiens

<400>  1

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
        35                  40                  45              


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys 
    50                  55                  60                  


Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
65                  70                  75                  80  


Leu Glu Asn Tyr Cys Asn 
                85      


<210>  2
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  2

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  3
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  3

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  4
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  4

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Glu Glu 
            20                  25                  30          


Gly Pro Arg Arg Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser 
        35                  40                  45              


Leu Glu Gln Leu Glu Asn Tyr Cys Asn 
    50                  55          


<210>  5
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  Misc Features
<222>  (28)..(28)
<223>  X is any amino acid

<220>
<221>  Misc Features
<222>  (28)..(29)
<223>  One or both amino acids is an amino acid other than Pro

<220>
<221>  Misc Features
<222>  (29)..(29)
<223>  X is any amino acid

<220>
<221>  Misc Features
<222>  (44)..(44)
<223>  X is Thr, His, Ser, Glu or Ala

<220>
<221>  Misc Features
<222>  (50)..(50)
<223>  X is any amino acid

<220>
<221>  Misc Features
<222>  (50)..(50)
<223>  X is any amino acid other than proline

<400>  5

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Xaa Thr Glu Glu 
            20                  25                  30          


Gly Pro Arg Arg Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser 
        35                  40                  45              


Leu Xaa Gln Leu Glu Asn Tyr Cys Asn 
    50                  55          


<210>  6
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  Misc Features
<222>  (31)..(31)
<223>  X is Glu or Ala

<220>
<221>  Misc Features
<222>  (32)..(32)
<223>  X is Glu or Ala

<220>
<221>  Misc Features
<222>  (33)..(33)
<223>  X is Gly or Ala

<220>
<221>  Misc Features
<222>  (34)..(34)
<223>  X is Pro or Ala

<220>
<221>  Misc Features
<222>  (35)..(35)
<223>  X is Arg or Ala

<220>
<221>  Misc Features
<222>  (36)..(36)
<223>  X is Arg or Ala

<220>
<221>  Misc Features
<222>  (44)..(44)
<223>  X is Thr, His, Ser, Glu or Ala

<220>
<221>  Misc Features
<222>  (50)..(50)
<223>  X is any amino acid other than Pro

<400>  6

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser 
        35                  40                  45              


Leu Xaa Gln Leu Glu Asn Tyr Cys Asn 
    50                  55          


<210>  7
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  Misc Features
<222>  (31)..(36)
<223>  X is 4 to 5 amino acids

<220>
<221>  Misc Features
<222>  (31)..(31)
<223>  X is Glu or Ala or is deleted

<220>
<221>  Misc Features
<222>  (32)..(32)
<223>  X is Glu or Ala or is deleted

<220>
<221>  Misc Features
<222>  (33)..(33)
<223>  X is Gly or Ala or is deleted

<220>
<221>  Misc Features
<222>  (34)..(34)
<223>  X is Pro or Ala or is deleted

<220>
<221>  Misc Features
<222>  (35)..(35)
<223>  X is Arg or Ala or is deleted

<220>
<221>  Misc Features
<222>  (36)..(36)
<223>  X is Arg or Ala or is deleted

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  Misc Features
<222>  (50)..(50)
<223>  X is any amino acid other than Pro

<400>  7

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser 
        35                  40                  45              


Leu Xaa Gln Leu Glu Asn Tyr Cys Asn 
    50                  55          


<210>  8
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  8

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Glu Glu 
            20                  25                  30          


Gly Ala Pro Arg Arg Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys 
        35                  40                  45              


Ser Leu Glu Gln Leu Glu Asn Tyr Cys Asn 
    50                  55              


<210>  9
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  9

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Glu Glu 
            20                  25                  30          


Gly Ala Ala Pro Arg Arg Gly Ile Val Glu Gln Cys Cys His Ser Ile 
        35                  40                  45              


Cys Ser Leu Glu Gln Leu Glu Asn Tyr Cys Asn 
    50                  55                  


<210>  10
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  10

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Glu Glu 
            20                  25                  30          


Gly Ala Ala Ala Pro Arg Arg Gly Ile Val Glu Gln Cys Cys His Ser 
        35                  40                  45              


Ile Cys Ser Leu Glu Gln Leu Glu Asn Tyr Cys Asn 
    50                  55                  60  


<210>  11
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  11

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


