                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientficas (CSIC)
 
<120>  UNSPECIFIC PEROXYGENASE ENZYME VARIANTS FOR SELECTIVE FATTY ACID 
       EPOXIDATION OR HYDROXYLATION

<130>  PCT1641.1465

<150>  EP 19382479
<151>  2019-06-07

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Marasmius rotula unspecific peroxygenase enzyme, 
       rMroUPO

<400>  1

Ser Ala His Pro Trp Lys Ala Pro Gly Pro Asn Asp Ser Arg Gly Pro 
1               5                   10                  15      


Cys Pro Gly Leu Asn Thr Leu Ala Asn His Gly Phe Leu Pro Arg Asn 
            20                  25                  30          


Gly Arg Asn Ile Ser Val Pro Met Ile Val Lys Ala Gly Phe Glu Gly 
        35                  40                  45              


Tyr Asn Val Gln Ser Asp Ile Leu Ile Leu Ala Gly Lys Ile Gly Met 
    50                  55                  60                  


Leu Thr Ser Arg Glu Ala Asp Thr Ile Ser Leu Glu Asp Leu Lys Leu 
65                  70                  75                  80  


His Gly Thr Ile Glu His Asp Ala Ser Leu Ser Arg Glu Asp Val Ala 
                85                  90                  95      


Ile Gly Asp Asn Leu His Phe Asn Glu Ala Ile Phe Thr Thr Leu Ala 
            100                 105                 110         


Asn Ser Asn Pro Gly Ala Asp Val Tyr Asn Ile Ser Ser Ala Ala Gln 
        115                 120                 125             


Val Gln His Asp Arg Leu Ala Asp Ser Leu Ala Arg Asn Pro Asn Val 
    130                 135                 140                 


Thr Asn Thr Asp Leu Thr Ala Thr Ile Arg Ser Ser Glu Ser Ala Phe 
145                 150                 155                 160 


Phe Leu Thr Val Met Ser Ala Gly Asp Pro Leu Arg Gly Glu Ala Pro 
                165                 170                 175     


Lys Lys Phe Val Asn Val Phe Phe Arg Glu Glu Arg Met Pro Ile Lys 
            180                 185                 190         


Glu Gly Trp Lys Arg Ser Thr Thr Pro Ile Thr Ile Pro Leu Leu Gly 
        195                 200                 205             


Pro Ile Ile Glu Arg Ile Thr Glu Leu Ser Asp Trp Lys Pro Thr Gly 
    210                 215                 220                 


Asp Asn Cys Gly Ala Ile Val Leu Ser Pro Glu Leu 
225                 230                 235     


<210>  2
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of the invention which comprises the SEQ ID NO: 1 and the
       amino acid substitution I153T

<400>  2

Ser Ala His Pro Trp Lys Ala Pro Gly Pro Asn Asp Ser Arg Gly Pro 
1               5                   10                  15      


Cys Pro Gly Leu Asn Thr Leu Ala Asn His Gly Phe Leu Pro Arg Asn 
            20                  25                  30          


Gly Arg Asn Ile Ser Val Pro Met Ile Val Lys Ala Gly Phe Glu Gly 
        35                  40                  45              


Tyr Asn Val Gln Ser Asp Ile Leu Ile Leu Ala Gly Lys Ile Gly Met 
    50                  55                  60                  


Leu Thr Ser Arg Glu Ala Asp Thr Ile Ser Leu Glu Asp Leu Lys Leu 
65                  70                  75                  80  


His Gly Thr Ile Glu His Asp Ala Ser Leu Ser Arg Glu Asp Val Ala 
                85                  90                  95      


Ile Gly Asp Asn Leu His Phe Asn Glu Ala Ile Phe Thr Thr Leu Ala 
            100                 105                 110         


Asn Ser Asn Pro Gly Ala Asp Val Tyr Asn Ile Ser Ser Ala Ala Gln 
        115                 120                 125             


Val Gln His Asp Arg Leu Ala Asp Ser Leu Ala Arg Asn Pro Asn Val 
    130                 135                 140                 


Thr Asn Thr Asp Leu Thr Ala Thr Thr Arg Ser Ser Glu Ser Ala Phe 
145                 150                 155                 160 


Phe Leu Thr Val Met Ser Ala Gly Asp Pro Leu Arg Gly Glu Ala Pro 
                165                 170                 175     


Lys Lys Phe Val Asn Val Phe Phe Arg Glu Glu Arg Met Pro Ile Lys 
            180                 185                 190         


Glu Gly Trp Lys Arg Ser Thr Thr Pro Ile Thr Ile Pro Leu Leu Gly 
        195                 200                 205             


Pro Ile Ile Glu Arg Ile Thr Glu Leu Ser Asp Trp Lys Pro Thr Gly 
    210                 215                 220                 


Asp Asn Cys Gly Ala Ile Val Leu Ser Pro Glu Leu 
225                 230                 235     


<210>  3
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of the invention which comprises the SEQ ID NO: 1 and the
       amino acid substitutions I153F and S156F

<400>  3

Ser Ala His Pro Trp Lys Ala Pro Gly Pro Asn Asp Ser Arg Gly Pro 
1               5                   10                  15      


Cys Pro Gly Leu Asn Thr Leu Ala Asn His Gly Phe Leu Pro Arg Asn 
            20                  25                  30          


Gly Arg Asn Ile Ser Val Pro Met Ile Val Lys Ala Gly Phe Glu Gly 
        35                  40                  45              


Tyr Asn Val Gln Ser Asp Ile Leu Ile Leu Ala Gly Lys Ile Gly Met 
    50                  55                  60                  


Leu Thr Ser Arg Glu Ala Asp Thr Ile Ser Leu Glu Asp Leu Lys Leu 
65                  70                  75                  80  


His Gly Thr Ile Glu His Asp Ala Ser Leu Ser Arg Glu Asp Val Ala 
                85                  90                  95      


Ile Gly Asp Asn Leu His Phe Asn Glu Ala Ile Phe Thr Thr Leu Ala 
            100                 105                 110         


Asn Ser Asn Pro Gly Ala Asp Val Tyr Asn Ile Ser Ser Ala Ala Gln 
        115                 120                 125             


Val Gln His Asp Arg Leu Ala Asp Ser Leu Ala Arg Asn Pro Asn Val 
    130                 135                 140                 


Thr Asn Thr Asp Leu Thr Ala Thr Phe Arg Ser Phe Glu Ser Ala Phe 
145                 150                 155                 160 


Phe Leu Thr Val Met Ser Ala Gly Asp Pro Leu Arg Gly Glu Ala Pro 
                165                 170                 175     


Lys Lys Phe Val Asn Val Phe Phe Arg Glu Glu Arg Met Pro Ile Lys 
            180                 185                 190         


Glu Gly Trp Lys Arg Ser Thr Thr Pro Ile Thr Ile Pro Leu Leu Gly 
        195                 200                 205             


Pro Ile Ile Glu Arg Ile Thr Glu Leu Ser Asp Trp Lys Pro Thr Gly 
    210                 215                 220                 


Asp Asn Cys Gly Ala Ile Val Leu Ser Pro Glu Leu 
225                 230                 235     


<210>  4
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  4

Met Lys Leu Ala Ile Ser Ser Ser Leu Ile Ala Leu Val Ser Val Thr 
1               5                   10                  15      


Thr Ala Leu Ala Asn Ser Gln Asp Val Val Asp Phe 
            20                  25              


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for the I153S mutation

<400>  5
ccgatttaac tgcgacttcc cgctcttcag aatctg                                 36


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for the I153T mutation

<400>  6
ccgatttaac tgcgactacc cgctcttcag aatctg                                 36


<210>  7
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for the I153V mutation

<400>  7
ccgatttaac tgcgactgtc cgctcttcag aatctg                                 36


<210>  8
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for the I153F/S156F mutation

<400>  8
ccgatttaac tgcgactttc cgctctttcg aatctgcg                               38


<210>  9
<211>  795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence that encodes the enzyme shown in SEQ ID 
       NO: 1

<400>  9
atgaaactgg cgatctcttc cagcctgatc gccctcgtct cggttaccac agctctggct       60

aacagccaag acgtagttga cttctccgca catccgtgga aagctccggg tccgaacgac      120

tctcgcggtc cgtgcccagg cctgaacact ctggctaacc acggcttcct gccgcgtaat      180

ggtcgtaaca tctccgtacc gatgattgtg aaagccggct tcgagggtta caacgtccag      240

agcgacatcc tgatcctcgc cggtaaaatc ggtatgctga cgtcgcgtga agctgatacc      300

atatcgttgg aagatctgaa actgcacggt accatcgaac acgatgcatc tctgagccgc      360

gaggatgtag cgattggtga taacctgcac ttcaacgagg caattttcac caccctggct      420

aactccaacc ctggtgcaga cgtttataac atttctagcg cggctcaggt gcagcatgat      480

cgtctggcag actctctggc gcgcaacccg aacgttacta acaccgattt aactgcgact      540

atccgctctt cagaatctgc gttcttcctg accgttatgt ccgcaggcga tccgctgcgt      600

ggtgaagcgc cgaagaaatt cgtgaacgtc ttctttcgtg aagaacgcat gccgattaaa      660

gaaggttgga aacgttccac caccccgatc actattccgc ttctgggtcc gatcattgag      720

cgcatcacgg aattatcgga ctggaaacca actggcgata actgcggcgc tatcgttctc      780

agcccggaac tgtaa                                                       795


