                         SEQUENCE LISTING

<110>  Wageningen Universiteit
 
<120>  Type III CRISPR/Cas-based diagnostics

<130>  P123796PC00

<140>  PCT/NL2020/050400
<141>  2020-06-19

<150>  EP 19181371.6
<151>  2019-06-19

<150>  EP 20168487.5
<151>  2020-04-07

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c-myc domain tag

<400>  1

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hemagglutinin tag

<400>  2

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW primer RfaH

<400>  3
tacgcccgcc gttgac                                                       16


<210>  4
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer RfaH

<400>  4
agccagcagg cgcaaa                                                       16


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CY5 oligo RfaH

<400>  5
aacaggacga atactgacgc gcca                                              24


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer CopG

<400>  6
cgaagaagac ggcatggaat                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer CopG

<400>  7
cgcagatccg gaggtcatta                                                   20


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YY oligo CopG

<400>  8
tcaacgtcag ctacgcaagg agtg                                              24


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 3 Pgi

<400>  9
gaaggtgaag atgttcataa tcacg                                             25


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 2 Pgi

<400>  10
cgtgaaatca cgccgttcag                                                   20


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 3 Pgi

<400>  11
gcgtgaaatt aagccgttca g                                                 21


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YY oligo Pgi

<400>  12
catacagggc aatcagcgcg cc                                                22


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 1 MrkC

<400>  13
atgtgtatac caatggtgag tggaa                                             25


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 1 MrkC

<400>  14
gcccattttg ccgtctttt                                                    19


<210>  15
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM oligo MrkC

<400>  15
ccgctacgac ctgaatatca cccgtg                                            26


<210>  16
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 1 Cfa

<400>  16
cctggtggcc gggatt                                                       16


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 1 Cfa

<400>  17
gtccagcgca ttcagatgag t                                                 21


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 3 Cfa

<400>  18
ggcatcaagc gcattaagat gtat                                              24


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM oligo Cfa

<400>  19
ccgcaaccct ttccgatctg gag                                               23


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 1 Xx

<400>  20
acaaaggagt cgggatgagt tc                                                22


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 1 Xx

<400>  21
cgaccattgc tcgtaaggct                                                   20


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM oligo Xx

<400>  22
caatcccagg ccaaatcacc gg                                                22


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 2 Omp

<400>  23
cccatgcttc agctttgtca                                                   20


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer 2 Omp

<400>  24
ctgcaggtta cgctaactcc aa                                                22


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM oligo Omp

<400>  25
cgttgccgtc acgtttctgg tcaa                                              24


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 1 OprL

<400>  26
gcgtcgagct gaagaagtaa gaag                                              24


<210>  27
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer OprL

<400>  27
ggctgagcgc gaggaa                                                       16


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CY5 oligo OprL

<400>  28
cccaagcacc tgcgtgtcct gac                                               23


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer 2 RecG

<400>  29
gcgaaatgga tgtatcaatc attg                                              24


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer RecG

<400>  30
tttccatcca ttctaaaaca gtatctaact                                        30


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YY oligo RecG

<400>  31
acacgttgga ttcggccgcc                                                   20


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fw primer Hns

<400>  32
gcacgtttag cacgaccagt t                                                 21


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rv primer Hns

<400>  33
tgccgatggt attgatccaa                                                   20


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM oligo Hns

<400>  34
cgccagcagc ttcaagcagg tca                                               23


<210>  35
<211>  53
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA 4.5, 46nts

<400>  35
auugcgaccc guagauaagg cgcccgggga cgaccacguc aaggcgcagu ugc              53


<210>  36
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA 4.5, 34nts

<400>  36
auugcgaccc guagauaagg cgcccgggga cgac                                   34


<210>  37
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA 4.5, 40nts

<400>  37
auugcgaccc guagauaagg cgcccgggga cgaccacguc                             40


<210>  38
<211>  46
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA 4.5, 46nts

<400>  38
auugcgaccc guagauaagg cgcccgggga cgaccacguc aaggcg                      46


<210>  39
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA 1

<400>  39
gaacugcgcc uugacguggu cguccccggg cgccuuaucu acggccaucg                  50


<210>  40
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Matched -3/-1 target RNA

<400>  40
guugugcgcc uugacguggu cguccccggg cgccuuaucu acggguccgu                  50


<210>  41
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Matched -4/-2 target RNA

<400>  41
guugugcgcc uugacguggu cguccccggg cgccuuaucu acggcucggu                  50


<210>  42
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Matched -5/-3 target RNA

<400>  42
guugugcgcc uugacguggu cguccccggg cgccuuaucu acggcacgcu                  50


<210>  43
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +1 RNA target

<400>  43
gaacugcgcc uugacguggu cguccccggg cgccuuaucu acgcccaucg                  50


<210>  44
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +2 RNA target

<400>  44
gaacugcgcc uugacguggu cguccccggg cgccuuaucu accgccaucg                  50


<210>  45
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +3 RNA target

<400>  45
gaacugcgcc uugacguggu cguccccggg cgccuuaucu agggccaucg                  50


<210>  46
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +4 RNA target

<400>  46
gaacugcgcc uugacguggu cguccccggg cgccuuaucu ucggccaucg                  50


<210>  47
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +5 RNA target

<400>  47
gaacugcgcc uugacguggu cguccccggg cgccuuauca acggccaucg                  50


<210>  48
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +6 RNA target

<400>  48
gaacugcgcc uugacguggu cguccccggg cgccuuaugu acggccaucg                  50


<210>  49
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +7 RNA target

<400>  49
gaacugcgcc uugacguggu cguccccggg cgccuuaacu acggccaucg                  50


<210>  50
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +1-5 RNA target

<400>  50
gaacugcgcc uugacguggu cguccccggg cgccuuauca ugccccaucg                  50


<210>  51
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +7-11 RNA target

<400>  51
gaacugcgcc uugacguggu cguccccggg cgcgaauacu acggccaucg                  50


<210>  52
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +13-17 RNA target

<400>  52
gaacugcgcc uugacguggu cguccccccc gcccuuaucu acggccaucg                  50


<210>  53
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +19-23 RNA target

<400>  53
gaacugcgcc uugacguggu ccagggcggg cgccuuaucu acggccaucg                  50


<210>  54
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +25-29 RNA target

<400>  54
gaacugcgcc uugaccacca cguccccggg cgccuuaucu acggccaucg                  50


<210>  55
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +31-35 RNA target

<400>  55
gaacugcgcg aacucguggu cguccccggg cgccuuaucu acggccaucg                  50


<210>  56
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA 2

<400>  56
gaacugcgcc uugacguggu cguccccggg cgccuuaucu acggccaucg                  50


<210>  57
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +11 RNA target

<400>  57
guugugcgcc uugacguggu cguccccggg cgcguuaucu acggcagcgu                  50


<210>  58
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +17 RNA target

<400>  58
guugugcgcc uugacguggu cgucccccgg cgccuuaucu acggcagcgu                  50


<210>  59
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +23 RNA target

<400>  59
guugugcgcc uugacguggu ccuccccggg cgccuuaucu acggcagcgu                  50


<210>  60
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +29 RNA target

<400>  60
guugugcgcc uugaccuggu cguccccggg cgccuuaucu acggcagcgu                  50


<210>  61
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Mismatched +35 RNA target

<400>  61
guugugcgcg uugacguggu cguccccggg cgccuuaucu acggcagcgu                  50


<210>  62
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  target RNA


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  desoxy-based nucleotide

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  desoxy-based nucleotide

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  desoxy-based nucleotide

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  desoxy-based nucleotide

<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  desoxy-based nucleotide

<400>  62
gaacugcgcc uugacguggu cguccccggg cgccuuaucu acggccaucg                  50


<210>  63
<211>  46
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA 4.5, 46nts

<400>  63
auugcgaccc guagauaagg cgcccgggga cgaccacguc aaggcg                      46


<210>  64
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA 1. This RNA was also used as off-target in the 
       norovirus assays.

<400>  64
gaacugcgcc uugacguggu cguccccggg cgccuuaucu acggccaucg                  50


<210>  65
<211>  46
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA norovirus, 46nts

<400>  65
auugcgacac uuaagccuuc aguuggaaaa uuugguggga cugcug                      46


<210>  66
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target RNA norovirus

<400>  66
gcaaguaauu cucgcgggga acgcguucac cgccgggaag aucauauuug cagcaguccc       60

accaaauuuu ccaacugaag gcuuaagucc uagccagguc acuauguucc cccaua          116


<210>  67
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Off-target RNA

<400>  67
cguaaacgac ggccagugcc aagcuuacua uacaaccuac uaccucauca                  50


<210>  68
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA_N3

<400>  68
auugcgacgc agcattgtta gcaggattgc gggtgccaat gtgatc                      46


<210>  69
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCOV_N3_RPA_F1

<400>  69
ggcataatac gactcactat agggtctgat aatggacccc aaaatcagcg aaat             54


<210>  70
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nCOV_N3_RPA_R1

<400>  70
ctccattctg gttactgcca gttgaatctg                                        30


<210>  71
<211>  1755
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb160 optimized

<400>  71
atggaacact tgctggcgat cgccttgggt ccggtccaag aatttatcgc gacagcccgt       60

cgcacacgtg atttgtatgc ggggtcacgt ttgttgagtg aggcagccgc gcgtgcagct      120

aaagcattgg cgcgtgaagt aggggcaaaa aaccttatct tccccgctcc cgaggatgag      180

gctgggctgg agcgtttagc gggggctggg attccgaacg ttttgcttgt tcgtgtcccg      240

gaaggtaaag atccgcgtgg gttgggcgag caggcacttg gggcggcacg tgactattta      300

cgcgaacgcg ctgaggaagt cttaggtccc cgtcgcgatc ttttattctg gcgtgaggct      360

ttagcacaag tggaggacct gttagaaggc tattacgcgt atcttccttt agaaggcgac      420

tatcctcgcg cgcgcgagcg tctgatggcc cttttggcgg cccgcaagaa cacacgcgat      480

tttgccccgg tttcttgggg ctcccccgct tataagtctt cccttgacgg tgcacgcgag      540

agcgtattgc gtttaccaga aaaggaagcg gaccatcttc gcgttcgtct ggggttacgc      600

cccggggaat acctggccgg gccggacctg ttaaagcgtt ggtggaaggc aggtcatggg      660

ttcttaagta ctacccacat ggcagcactg ccattttggg aaggggttcg ccgcgcaggg      720

cttgaagccg tgcttaaaga ggcgctggag gaacttgggg gtttagttgg cgaggaggcc      780

cgtgcggagg ttcgtcatcc agttctgcgt gatactccat ttggcgaatg ggacgtacgt      840

cttttgtacg aaagccgttt agaggagttt ccgtcattag ccgaagaccc cgggttattg      900

gagaaagctc gcgatcgcct gcgtgcgtta tggcgtcgct tgtttcccaa ggttcgcgta      960

ccaccagggg catactatgc gctgttgcac gccgatggcg atcgcatggg agaaacattg     1020

gacggattgc cttcaccaga agctcatcgc cgcttttcaa atgcgcttgc attagggttt     1080

gccgcgcagg tcaaggacat tgtggaagct catggtggag ggttagtata ttcaggcggg     1140

gacgatgtgt tagccctgct tccactgcac acagccttaa tggcggcacg tgctttagca     1200

gatcgcttcc gcgaagctat ggcacctttt gggcgcgaag gccgcgcgcc ctctttatcc     1260

gtagggcttg cagtagtaca ccacttggag cctctgcaag acgcattgga tcttgcgcgc     1320

cgcgcggaaa agtgggctaa agaaggtgaa ccaaagcgta atgcattgtg cgttgcctat     1380

tccccgcgta gtggagcaga acgtttagta cgtggccgtt gggacgagaa tccgccactg     1440

actcgccgct tgctgcgtta cgccgacctg ttacgcgccg gggaggtgcc tagtcgcgcc     1500

gcatacgagt tgttagcact ggtccgcgaa gctggcgagg ccttgtcagg ggaagccctg     1560

gtagctgagg ccttacgtat cttgggtcgc aaagaaatga aacgtgctta ccgtgaagag     1620

ttggaagcct ggctgcgcac tggcgaagat gttcgtcgcc tggcagagga attaattctt     1680

gctcgccctt tcgcagaggc cctggaccaa gctggcgtgc ccgtcgagag tcgtgaagtg     1740

tgggacgcgc attaa                                                      1755


<210>  72
<211>  1086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb161 optimized

<400>  72
atgctcatcg agcctcgcga tccgctgatt gtgcgcgacg gacgcccctt tactaacagt       60

cctggtgcgc gcgcgaaatc gttaccattt ccgctgccgc agacattggc tggtgcctac      120

cgtacccgcc gcgcgctttt ggaggggttg ccacttccgg aacgtgcgga ggaggtctta      180

cgctggggtc ttcgtggccc gctgttggcc gaagagggtg aggggggttg gcgtctgtta      240

gcacctcgcc cgttggatgc gctgaagctt ggcgaggcac tgtatccctt gcgccctctt      300

gagttgccgc aaggtgcggg taccaacctg cccgaggggt tatctcccgt tggcctgcca      360

agcccggcgc ttaaagaaaa gccggccccg ttgccggcat tttggtactg ggagtctttc      420

cttgagtggt tgcttcaaga tgcccctgca ggttttgcgc cccgtggaca tgaaggtcct      480

gtgcctgaga cacgtacaca tgtcgcactt gacccagcag cacaaaccgc gcgtgaggga      540

ttcttattcc aaacatcggg gcttgaattt gttcgtggac gccgtcgtct tgctctggtg      600

ttgtggcccg aagggcccga gccagaagga gttttcccac ttggtggaga acgccgctta      660

gctttttggc aaaaaggggg cccgggggtg cctcctttac cagaggaagt cgttgctggc      720

ctgttacgtc atcgtgcagc gcgtcttgta tttcttacac ccgcgtttct gggcgaggct      780

taccttccaa agggtcgcgc cttccaaggc gctagcgtgg tggcggccgt cgtaggccgt      840

ccattagccg tgtccggatg gaatttgaag gaaggtaaac cgaaaccttc ccgtcgcgcc      900

gtgcctgctg ggagcgtgta cttcgtccgt cttccggaag cgtggggtga aggagaggta      960

cgtgactggg cggagaaagt ctggttccaa aacctttccg aggaggaaca ggatcgtcgt     1020

gacgggttcg ggcttgccgc gttaggcctt tgggatggga aacttcgtcg ctgggaagag     1080

gcctga                                                                1086


<210>  73
<211>  1188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb162 optimized

<400>  73
atgagacgcg catttcgcga ggcattctcc ccccgtcgca aggaagaggg tggtgttctt       60

gtgcgccgcg atggcgctcg tgtgctggct tgggaacgta cgtatactct tttgacaccg      120

ttgtttggcg gaggtgttga accacgtgaa gcggacccag tgtcagtggt gcgcgcaacc      180

gcagtccgcg ggcacttacg tttctggtgg cgtgctgttc gcggctggcg tgcaggagga      240

tctcttgaac gcttgtggga gcttgagtcc gcattgtttg ggtcagctgg agaagggggg      300

gcatcaccgc tgtcggttga agttgaggtc ttacgtgagg gggaaaaggt gggtattgct      360

caatacgggc gtgcggttca atggtacctg ggtttcccac ttcgtggcga caaagagtgg      420

gcaccagtaa aagagggggt agcgtttcgt cttcgcctgc gctttcccga gaaagtgggg      480

gagcttaatt tctgggagga attggaggcg gcactttggg cttgggaaac atttgggggc      540

attggtgcgc gtacgcgccg tggattcggc gcattattac cgcaaggggc tggtgttccc      600

ggggaagaag aaattcgtga gaaacttcgc caatatggac aaaaagcggg gtggccggaa      660

ggcgttccgc acttgacgcc aaaaagcttt gtgcgtgtgg tgcctttatc gtggcgtgag      720

ctggccgagc gctatcaggc ttttcgtcag gctcgcccgg gcggagatcc gcgcagtccc      780

ggtcgcagct actggccgga acctgatggc gtacgtcgct tgacagggcg tcacgcacct      840

catcatttgc cacgccatcc agtacataaa tttccccgcg ctcatttcgg cctgccaatc      900

attttccatt tcaaagaccg tggtgatccc ccagatacta ccttgcgcct taaagaagct      960

gaccgccgcg cctctccgtt gttatttcgc ccattgggcg agggtcaaaa accctgcgtt     1020

gtggccgtct tggagggggc ccgctttccc ggggagaaat tggttttgga aggaaaggat     1080

ggtcgtacat gggatgtgga tccctggttg acccccgaag aggcacagaa gattcccgta     1140

ttgggtggcg aagtggatcc tgttttagca tttgttaagt ctctgtag                  1188


<210>  74
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb163 optimized

<400>  74
atgagccatg tcgcattgtt attcttacat gcgttatctc ccctgcatgc cgggactggc       60

caaggcatcg gtgccattga cttgcctatc gcacgtgaga aggcaactgg gattccctac      120

ttacccggaa gttcgcttaa aggcgtgttg cgcgaccgcg ccagtgcctg ggaccgcgat      180

accttgttcg cagtatttgg tccagatacg gagaacgcta gcgagcacgc aggcgccgtt      240

caggtcggtg acgccaagtt attattatta ccggtgcgta gtttatacgg tgtattcgct      300

ttagttgcgt ctccgtactt gctggagcgt ttccgccgcg aggccttgat ggctggtctt      360

caaccgcctg gggttcctgg tttacaagat cctactcgcg ttctgttggc accaggatca      420

cgcttacttg gagatgggga gaaagtgtac ttagaggacc tggacttaaa agcgcagggg      480

gaggaaggag ttgccgcctg ggagcgttgg ctggcagaac gtactgaggc gccagttttg      540

ggacgcctgg ctgtagtaca tgatgacctt atgggttttc ttcttgagac agccaccgaa      600

gtagtagccc gtatccgtct ggacgatgaa accaaaacag tcgccaaagg cgctttgtgg      660

tatgaggaat cccttcccgc cgaatcgttg ctttacgcat tagtccgtgc tgaccgctcg      720

ttccgcaagg gcaaggaatt gccaccagag ggagtgtggg cactttttcg cggtgtcctt      780

gaagagggtg ggggtgtgtt gcagcttgga ggcaaggcta cggtagggcg tggcctgtgt      840

cgcatcgtag tcgggcgtta g                                                861


<210>  75
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb164 optimized

<400>  75
atgcgcacac gtagtcaagt ctgggcccaa aaggcctatg agaaggtacg tgaagctgcc       60

aagggagagg gccgcgggga ataccgtgac atggccctga agttgcctgt tcttgtgcgt      120

caggcaggtc tgtcccaggc attggcgttc gtcgatagtc gtggcaagga ggcacataag      180

gcccttggta atgatttagc gcaggtgtta ggataccgcg atctgcgcga attggcggag      240

gcggctcgtg aagcggagct tctgcaatac cttcgtttga cgcgcgaagt gcttgctgcc      300

gcggagtggt tcaaacgttt cgcccaagcg ttgatcgaag agtag                      345


<210>  76
<211>  1047
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tthb165 optimized

<400>  76
atgggccgtc gttccgcctt ggagggagta cgcttgcctc aaggcaagga accgcatcgt       60

ggcctttggc tggacaaatt cttacgttcg gcacgccgcg aagatacaga ggctaaacgc      120

gtccttgttc gtgaagcggc gggtattccg gagcctggtg aatatcgcgc cttcttcaag      180

cgctatcgtg gtgcgctgga agctttgggt gcggaaattc gcgaagcccg cactctttct      240

cgcttggtgg tcggtttagg aggcgaaggg gttcttgaga ctgctttaac cctgcatcgt      300

gcttatgggg tgccctatat tcctggcagc gcacttaagg gcttagctag ccgctacgct      360

cacctgtact tggagggtga agcctggcgt cgcgatctgg cgcgttttca ccgcggggag      420

gcgcaggctg gcctgttcgg gacaacggag gaacaaggtt tggttgtctt ttgggatgcc      480

cttccccttc cagggaaatg gaagctgcat cctgatattc ttaatccgca tcacccagat      540

tactacgggt cagttaaagc tccacccgcc gactgggacg gtcccaaacc tgttcctttc      600

ttgagcgcaa ccggaacgtt cttattggcg ttatctccag ctcccggtgt gtcaccggag      660

gaagccgggc catggcttcg cgctgcttgg cgtatcttag cttgggccct tcgtgaggaa      720

ggagtcgggg caaaaacatc ctccggatac ggccgtatgg cattagaaga acccgcctct      780

cagggggaaa agccattagc cccaggtcct tcgcctgtcc tgcaagactt actgacttgg      840

gctcgcgcgt taagttaccg tgaggttccg cgctttttag ccagccaagc ggaggcgatc      900

ttgggcttgt cggttgagga agctcaagca cttcgccgcg cacttgagga gcgcggtttc      960

ttacgtaacc cgcaggatct taaacgctgg cgtaaggagc acccgggact tgagggggta     1020

ttagcgaagc ttggtcttag tgcatag                                         1047


<210>  77
<211>  72
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pf_Cmr4

<400>  77

Met Lys Ala Tyr Leu Val Gly Leu Tyr Thr Leu Thr Pro Thr His Pro 
1               5                   10                  15      


Gly Ser Gly Thr Glu Leu Gly Val Val Asp Gln Pro Ile Gln Arg Glu 
            20                  25                  30          


Arg His Thr Gly Phe Pro Val Ile Trp Gly Gln Ile Asn Ala Glu Thr 
        35                  40                  45              


Gly Thr Val Glu Thr Gly Gly Leu Trp Tyr Glu Glu Tyr Ile Pro Ser 
    50                  55                  60                  


Asp Thr Leu Phe Tyr Ser Leu Ile 
65                  70          


<210>  78
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tm_Cmr4

<400>  78

Met Ser His Val Ala Leu Leu Phe Leu His Ala Leu Ser Pro Leu His 
1               5                   10                  15      


Ala Gly Thr Gly Gln Gly Ile Gly Ala Ile Asp Leu Pro Ile Ala Arg 
            20                  25                  30          


Glu Lys Ala Thr Gly Ile Pro Tyr Leu Pro Gly Ser Ser Leu Asp Asp 
        35                  40                  45              


Glu Thr Lys Thr Val Ala Lys Gly Ala Leu Trp Tyr Glu Glu Ser Leu 
    50                  55                  60                  


Pro Ala Glu Ser Leu Leu Tyr Ala Leu Val 
65                  70                  


<210>  79
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tt_Cmr4

<400>  79

Met Glu Gly Arg Val Phe Leu Leu Tyr Ala Glu Thr Gln Val His Ala 
1               5                   10                  15      


Gly Thr Gly Phe Glu Ile Gly Val Val Asp Leu Pro Ile Gln Arg Glu 
            20                  25                  30          


Arg Thr Thr Lys Phe Pro Ile Ile His Gly Asn Arg Glu Lys Lys Thr 
        35                  40                  45              


Val Glu Glu Gly Gly Leu Trp Tyr Glu Glu Tyr Leu Pro Gln Asp Thr 
    50                  55                  60                  


Val Met Tyr Phe Val Val 
65                  70  


<210>  80
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SSo_Cmr4

<400>  80

Met Ile Ile Met Thr Tyr Tyr Thr Phe Val Lys Pro Phe Phe Ile Lys 
1               5                   10                  15      


Ala Val Thr His Leu His Val Ser Ser Gly Thr Ser Val Glu Glu Glu 
            20                  25                  30          


Val Asp Leu Pro Phe Gln Arg Asp Glu Leu Asn Tyr Pro Thr Ile Phe 
        35                  40                  45              


Ala Ser Ser Leu Lys Arg Ser Ser Lys Thr Val Glu Glu Gly Gly Leu 
    50                  55                  60                  


Trp Ser Glu Glu Tyr Val Pro Val Asn Ser Leu Phe Phe Ser Ser Phe 
65                  70                  75                  80  


<210>  81
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pf_Cmr4

<400>  81

Met Lys Ala Tyr Leu Val Gly Leu Tyr Thr Leu Thr Pro Thr His Pro 
1               5                   10                  15      


Gly Ser Gly Thr Glu Leu Gly Val Val Asp Gln Pro Ile Gln Glu Leu 
            20                  25                  30          


Ser Thr Glu Ile Val Ala Arg Ile Arg Ile Asn Ala Glu Thr Gly Thr 
        35                  40                  45              


Val Glu Thr Gly Gly Leu Trp Tyr Glu Glu Tyr Ile Pro Ser Asp Thr 
    50                  55                  60                  


Leu Phe Tyr 
65          


<210>  82
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mk_Csm3

<400>  82

Met Val Gly Ile Gly Gly Thr Ile Thr Leu Val Gly Glu Ile Arg Leu 
1               5                   10                  15      


Arg Thr Gly Thr Arg Ile Gly Thr Ser Glu Glu Glu Ile Glu Ile Gly 
            20                  25                  30          


Gly Leu Asp Asn Pro Val Ile Gly Glu Pro Thr Glu Val Lys His Glu 
        35                  40                  45              


Asn Ala Ile Asn Arg Val Ser Gly Glu Ala Asn Pro Arg Ser Met Glu 
    50                  55                  60                  


Arg Val Pro Lys Gly Ser Arg Phe Gly Leu 
65                  70                  


<210>  83
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ph_Csm3

<400>  83

Met Asn Arg Glu Phe Tyr Gly Lys Ile Ile Ile Ser Gly Glu Ile Glu 
1               5                   10                  15      


Ala Val Thr Gly Leu His Ile Gly Ser Gln Arg Glu Val Ser Glu Ile 
            20                  25                  30          


Gly Gly Ile Asp Asn Pro Val Ile Ala Ile Thr Glu Ala Lys Ile Glu 
        35                  40                  45              


Val Gly Ile Asp Arg Val Thr Ser Gln Ala Asn Pro Arg Thr Thr Glu 
    50                  55                  60                  


Arg Val Val Ala Gly Thr Arg Phe Asp Phe 
65                  70                  


<210>  84
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tt_Csm3

<400>  84

Met Lys Leu Lys Lys Val Ile Arg Ile Arg Ser Val Leu Leu Ala Lys 
1               5                   10                  15      


Thr Gly Leu Arg Ile Gly Met Ser Arg Asp Gln Met Ala Ile Gly Asp 
            20                  25                  30          


Leu Asp Asn Pro Val Val Gly Leu Tyr Thr Glu Ile Lys Gln Glu Val 
        35                  40                  45              


Phe Ile Pro Arg Leu Gly Gly Asn Ala Asn Pro Arg Thr Thr Glu Arg 
    50                  55                  60                  


Val Pro Ala Gly Ala Arg Phe Arg Val 
65                  70              


<210>  85
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tm_Csm3

<400>  85

Met Glu Arg Pro Ile Leu Gly Lys Tyr Ile Ile Lys Gly Lys Ile Ile 
1               5                   10                  15      


Leu Glu Thr Gly Leu Arg Ile Gly Gly Gln Glu Leu Gly Val Asn Ile 
            20                  25                  30          


Gly Gly Ile Asp Asn Pro Val Ile Pro Tyr Thr Glu Trp Lys Thr Glu 
        35                  40                  45              


Asn Ala Leu Asp Arg Val Thr Cys Lys Ala Asp Pro Arg Ser Phe Glu 
    50                  55                  60                  


Arg Ile Pro Ala Gly Ala Glu Phe Glu Phe 
65                  70                  


<210>  86
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Si_Csm3

<400>  86

Met Ser Ser Gln Pro Ser Ile Ser Leu Lys Leu Glu Lys Ile Ile Arg 
1               5                   10                  15      


Phe Lys Val Tyr Leu Gln Thr Ile Thr Gly Leu Leu Ile Ser Ala Gly 
            20                  25                  30          


Lys Ala Leu Gly Arg Ile Gly Gly Ala Asp Thr Glu Pro Met Ser Asp 
        35                  40                  45              


Phe Ile Glu Asp Lys Asn Glu Asn Arg Ile Asp Arg Val Thr Ser Ala 
    50                  55                  60                  


Ala Asp His Arg Thr Ile Gly Arg Val Lys Pro Gly Val Thr Phe Thr 
65                  70                  75                  80  


Gly 
    


