                         SEQUENCE LISTING

<110>  Rheinische Friedrich-Wilhelms-Universitt Bonn
 
<120>  ENZYMATIC PRODUCTION OF LEVAN-BASED, PREBIOTIC 
       FRUCTOOLIGOSACCHARIDES

<130>  P78546

<150>  EP19182814.4
<151>  2019-06-27

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  461
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of the modified levansucrase from 
       Gluconobacter japonicus LMG 1417

<400>  1

Met Ala Ser Trp Ser His Pro Gln Phe Glu Lys Gly Ala Glu Thr Ala 
1               5                   10                  15      


Glu Asn Ala Ile Ser Ser Arg Ile Leu Asp Gln His Met Ala Val Arg 
            20                  25                  30          


Arg Val Leu Pro Asn Tyr Arg Ser Ser Arg Trp Thr Ile Ala Asp Ala 
        35                  40                  45              


Leu Lys Val Arg Thr Asp Asp Pro Thr Thr Thr Met Pro Val Ile Asp 
    50                  55                  60                  


Tyr Ser Phe Pro Val Met Ser Asn Asp Val Met Ile Trp Asp Ser Gly 
65                  70                  75                  80  


Ala Leu Arg Asp Met His Met Arg Thr Val Thr Tyr Lys Gly Trp Phe 
                85                  90                  95      


Val Leu Trp Ser Leu Ala Val Asp Lys Ile Asp Lys Thr Asn Ala Lys 
            100                 105                 110         


Ile Tyr Asp Glu Trp His Ser Arg Asn Asp Arg Ala Tyr Ile Gly Tyr 
        115                 120                 125             


Trp Tyr Ser Ala Asp Gly Val Glu Trp Thr Trp Gly Gly Arg Leu Phe 
    130                 135                 140                 


Gln Thr Ser Ala Asp Leu Arg Pro Trp Glu Trp Ser Gly Ser Leu Val 
145                 150                 155                 160 


Met Arg Glu Gly Thr Glu Asn Lys Val Asp Met Phe Tyr Thr Ser Val 
                165                 170                 175     


Gly Ala Pro Asp Gly Asn Ser Val Pro Ala Val Cys Ser Gly Thr Ile 
            180                 185                 190         


His Ala Asp Asp Lys Gly Val Trp Phe Glu Gly Phe Ser Ala Ser Thr 
        195                 200                 205             


Glu Met Phe Lys Ala Asp Gly Val His Tyr Ala Asn Ala Ser Glu Asp 
    210                 215                 220                 


Ala Tyr Phe Asp Phe Arg Asp Pro Gln Pro Phe Ile Asn Pro Gly Asp 
225                 230                 235                 240 


Gly Glu Leu Tyr Thr Leu Phe Glu Gly Asn Val Pro Gly Met Arg Gly 
                245                 250                 255     


Gln Phe Val Val Gly Ser Asp Glu Met Gly His Val Pro Pro Gly Tyr 
            260                 265                 270         


Ala Val Asp Pro Gly Ala Gln Tyr Gly Ala Ala Ala Ile Gly Leu Ser 
        275                 280                 285             


Arg Cys Val Ser Asn Trp Arg Lys Gly Asp Tyr Ser Arg Trp Glu Leu 
    290                 295                 300                 


Met Pro Ala Leu Val Thr Ala Leu Gly Val Asn Asp Gln Thr Glu Arg 
305                 310                 315                 320 


Pro His Phe Val Phe Lys Asp Gly Leu Thr Tyr Leu Phe Thr Ile Ser 
                325                 330                 335     


His His Ser Thr Tyr Thr Gly Lys Ser Thr Gly Pro Asp Gly Val Tyr 
            340                 345                 350         


Gly Phe Val Ser Glu Lys Gly Ile Phe Gly Pro Tyr Lys Pro Leu Asn 
        355                 360                 365             


Ser Ser Gly Leu Val Leu Gly Asn Pro Ser Ser Gln Pro Tyr Ser Thr 
    370                 375                 380                 


Tyr Ser His Phe Val Asp Ala Gln Gly Tyr Val Gln Ser Phe Ile Asp 
385                 390                 395                 400 


Thr Ile Pro Ala Ala Gly Gln Asn Ala Glu Ser Pro Asp Thr Tyr Arg 
                405                 410                 415     


Ile Gly Gly Thr Leu Ala Pro Thr Val Arg Leu Val Leu Asp Gly Asp 
            420                 425                 430         


Arg Thr Phe Leu Thr Glu Val His Asp Tyr Ala Gln Val Phe Ala Gln 
        435                 440                 445             


Ala Ala Trp Pro Ala Ser Leu Ala Tyr Asp Val Arg Ala 
    450                 455                 460     


<210>  2
<211>  445
<212>  PRT
<213>  Gluconobacter japonicus LMG 1417

<400>  2

Met Asn Ala Ile Ser Ser Arg Ile Leu Asp Gln His Met Ala Val Arg 
1               5                   10                  15      


Arg Val Leu Pro Asn Tyr Arg Ser Ser Arg Trp Thr Ile Ala Asp Ala 
            20                  25                  30          


Leu Lys Val Arg Thr Asp Asp Pro Thr Thr Thr Met Pro Val Ile Asp 
        35                  40                  45              


Tyr Ser Phe Pro Val Met Ser Asn Asp Val Met Ile Trp Asp Ser Gly 
    50                  55                  60                  


Ala Leu Arg Asp Met His Met Arg Thr Val Thr Tyr Lys Gly Trp Phe 
65                  70                  75                  80  


Val Leu Trp Ser Leu Ala Val Asp Lys Ile Asp Lys Thr Asn Ala Lys 
                85                  90                  95      


Ile Tyr Asp Glu Trp His Ser Arg Asn Asp Arg Ala Tyr Ile Gly Tyr 
            100                 105                 110         


Trp Tyr Ser Ala Asp Gly Val Glu Trp Thr Trp Gly Gly Arg Leu Phe 
        115                 120                 125             


Gln Thr Ser Ala Asp Leu Arg Pro Trp Glu Trp Ser Gly Ser Leu Val 
    130                 135                 140                 


Met Arg Glu Gly Thr Glu Asn Lys Val Asp Met Phe Tyr Thr Ser Val 
145                 150                 155                 160 


Gly Ala Pro Asp Gly Asn Ser Val Pro Ala Val Cys Ser Gly Thr Ile 
                165                 170                 175     


His Ala Asp Asp Lys Gly Val Trp Phe Glu Gly Phe Ser Ala Ser Thr 
            180                 185                 190         


Glu Met Phe Lys Ala Asp Gly Val His Tyr Ala Asn Ala Ser Glu Asp 
        195                 200                 205             


Ala Tyr Phe Asp Phe Arg Asp Pro Gln Pro Phe Ile Asn Pro Gly Asp 
    210                 215                 220                 


Gly Glu Leu Tyr Thr Leu Phe Glu Gly Asn Val Pro Gly Met Arg Gly 
225                 230                 235                 240 


Gln Phe Val Val Gly Ser Asp Glu Met Gly His Val Pro Pro Gly Tyr 
                245                 250                 255     


Ala Val Asp Pro Gly Ala Gln Tyr Gly Ala Ala Ala Ile Gly Leu Ser 
            260                 265                 270         


Arg Cys Val Ser Asn Trp Arg Lys Gly Asp Tyr Ser Arg Trp Glu Leu 
        275                 280                 285             


Met Pro Ala Leu Val Thr Ala Leu Gly Val Asn Asp Gln Thr Glu Arg 
    290                 295                 300                 


Pro His Phe Val Phe Lys Asp Gly Leu Thr Tyr Leu Phe Thr Ile Ser 
305                 310                 315                 320 


His His Ser Thr Tyr Thr Gly Lys Ser Thr Gly Pro Asp Gly Val Tyr 
                325                 330                 335     


Gly Phe Val Ser Glu Lys Gly Ile Phe Gly Pro Tyr Lys Pro Leu Asn 
            340                 345                 350         


Ser Ser Gly Leu Val Leu Gly Asn Pro Ser Ser Gln Pro Tyr Ser Thr 
        355                 360                 365             


Tyr Ser His Phe Val Asp Ala Gln Gly Tyr Val Gln Ser Phe Ile Asp 
    370                 375                 380                 


Thr Ile Pro Ala Ala Gly Gln Asn Ala Glu Ser Pro Asp Thr Tyr Arg 
385                 390                 395                 400 


Ile Gly Gly Thr Leu Ala Pro Thr Val Arg Leu Val Leu Asp Gly Asp 
                405                 410                 415     


Arg Thr Phe Leu Thr Glu Val His Asp Tyr Ala Gln Val Phe Ala Gln 
            420                 425                 430         


Ala Ala Trp Pro Ala Ser Leu Ala Tyr Asp Val Arg Ala 
        435                 440                 445 


<210>  3
<211>  1386
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the modified levansucrase gene from 
       Gluconobacter japonicus LMG 1417

<400>  3
atggctagct ggagccaccc gcagttcgaa aaaggcgccg agaccgcgga aaatgctatt       60

tccagccgaa ttctggatca gcacatggct gttcgtcgcg tgcttccgaa ctatcgctcg      120

tcccggtgga cgattgctga tgcgctgaag gttcgtacgg acgatccgac gacgacaatg      180

ccggtaatcg actacagttt cccagtcatg tccaacgatg tgatgatctg ggacagtggc      240

gcccttcgtg acatgcatat gcgcactgtg acgtataagg gctggtttgt tctctggtct      300

ctggctgtgg acaaaatcga caagaccaat gccaagattt atgacgaatg gcactcccgc      360

aatgatcgcg cctatatcgg ctactggtac agcgccgacg gtgttgagtg gacatggggt      420

ggccgcctgt ttcagacgtc tgctgatctg cgtccctggg agtggtccgg ttctctggtg      480

atgcgtgaag gcaccgagaa caaggtcgac atgttctaca cgtccgttgg tgcgccggat      540

ggtaattctg ttccggctgt ttgctccggc accattcatg ctgatgacaa gggtgtctgg      600

tttgagggct tctcggccag cacggaaatg ttcaaggctg atggcgttca ttatgccaac      660

gcttctgaag atgcgtattt cgatttccgc gatccgcagc cgttcatcaa tccgggtgat      720

ggcgagcttt acacgctgtt cgaaggcaat gttcctggca tgcgggggca gtttgttgtc      780

ggttctgacg agatgggcca tgttccgcca ggttatgcgg tggatccggg tgcgcaatat      840

ggtgctgcgg caatcggtct ctcccggtgt gtgtctaact ggcgcaaggg tgattattcc      900

cgttgggagc tgatgcctgc tctggtgacg gctctgggcg tgaacgatca gacggagcgt      960

ccgcatttcg tcttcaagga cggtctgacc tatctgttca ccatcagcca tcattccacc     1020

tatacaggca aaagtacggg ccctgatggt gtgtatggtt tcgtgtccga gaaggggatt     1080

tttggtccct acaagcctct aaacagctcg ggtctggtac ttggtaaccc gtcttcacag     1140

ccgtattcga cgtattcgca ttttgtggat gcgcagggct acgtccagtc gtttatcgat     1200

acgattccgg cggctggtca gaacgctgaa agccctgata cctatcgtat tggcggcacg     1260

cttgctccga cggtgcggct ggttctggat ggtgatcgga cgttcctgac ggaagttcat     1320

gactatgcgc aggtttttgc gcaggccgcc tggccggctt ctctggccta tgacgttcgt     1380

gcctga                                                                1386


<210>  4
<211>  1338
<212>  DNA
<213>  Gluconobacter japonicus LMG 1417

<400>  4
atgaatgcta tttccagccg aattctggat cagcacatgg ctgttcgtcg cgtgcttccg       60

aactatcgct cgtcccggtg gacgattgct gatgcgctga aggttcgtac ggacgatccg      120

acgacgacaa tgccggtaat cgactacagt ttcccagtca tgtccaacga tgtgatgatc      180

tgggacagtg gcgcccttcg tgacatgcat atgcgcactg tgacgtataa gggctggttt      240

gttctctggt ctctggctgt ggacaaaatc gacaagacca atgccaagat ttatgacgaa      300

tggcactccc gcaatgatcg cgcctatatc ggctactggt acagcgccga cggtgttgag      360

tggacatggg gtggccgcct gtttcagacg tctgctgatc tgcgtccctg ggagtggtcc      420

ggttctctgg tgatgcgtga aggcaccgag aacaaggtcg acatgttcta cacgtccgtt      480

ggtgcgccgg atggtaattc tgttccggct gtttgctccg gcaccattca tgctgatgac      540

aagggtgtct ggtttgaggg cttctcggcc agcacggaaa tgttcaaggc tgatggcgtt      600

cattatgcca acgcttctga agatgcgtat ttcgatttcc gcgatccgca gccgttcatc      660

aatccgggtg atggcgagct ttacacgctg ttcgaaggca atgttcctgg catgcggggg      720

cagtttgttg tcggttctga cgagatgggc catgttccgc caggttatgc ggtggatccg      780

ggtgcgcaat atggtgctgc ggcaatcggt ctctcccggt gtgtgtctaa ctggcgcaag      840

ggtgattatt cccgttggga gctgatgcct gctctggtga cggctctggg cgtgaacgat      900

cagacggagc gtccgcattt cgtcttcaag gacggtctga cctatctgtt caccatcagc      960

catcattcca cctatacagg caaaagtacg ggccctgatg gtgtgtatgg tttcgtgtcc     1020

gagaagggga tttttggtcc ctacaagcct ctaaacagct cgggtctggt acttggtaac     1080

ccgtcttcac agccgtattc gacgtattcg cattttgtgg atgcgcaggg ctacgtccag     1140

tcgtttatcg atacgattcc ggcggctggt cagaacgctg aaagccctga tacctatcgt     1200

attggcggca cgcttgctcc gacggtgcgg ctggttctgg atggtgatcg gacgttcctg     1260

acggaagttc atgactatgc gcaggttttt gcgcaggccg cctggccggc ttctctggcc     1320

tatgacgttc gtgcctga                                                   1338


<210>  5
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of the modified endolevanase from Azotobacter
       chroococcum DSM 2286

<400>  5

Met Ala Ser Trp Ser His Pro Gln Phe Glu Lys Gly Ala Pro Ala Pro 
1               5                   10                  15      


Ala Thr Pro Ser Gly Pro Glu Pro Ser Ser Pro Pro Pro Ala Lys Ser 
            20                  25                  30          


Ser Glu Phe Ser Arg Pro Ala Asp Trp Ser Pro His Arg Pro Ala Val 
        35                  40                  45              


His Leu Thr Pro Ala Lys His Trp Met Asn Asp Pro Gln Arg Pro Ile 
    50                  55                  60                  


Leu Ile Asp Gly Val Trp His Tyr Tyr Tyr Leu Tyr Asn Ala Asp Tyr 
65                  70                  75                  80  


Pro Lys Gly Asn Gly Thr Glu Trp Tyr His Ala Thr Ser Thr Asp Leu 
                85                  90                  95      


Val His Trp Gln Asp His Gly Val Ala Ile Asp Lys Tyr Lys Asn Gly 
            100                 105                 110         


Leu Gly Asp Ile Glu Thr Gly Ser Ala Val Ile Asp Thr Glu Asn Thr 
        115                 120                 125             


Ala Gly Phe Gly Ala Gly Ala Val Ile Ala Val Met Thr Gln Gln His 
    130                 135                 140                 


Glu Gly Val Gln Arg Gln Ser Leu Phe Val Ser Thr Asp Gly Gly Tyr 
145                 150                 155                 160 


Arg Phe Lys Ala Tyr Asp Gly Asn Pro Val Met Asp Asn Pro Gly Ala 
                165                 170                 175     


Glu His Trp Arg Asp Pro Lys Ile Ile Trp Asp Asp Ala Arg Asp Glu 
            180                 185                 190         


Trp Leu Met Ala Leu Ala Glu Gly Asp Lys Ile Gly Phe Tyr Thr Ser 
        195                 200                 205             


Pro Asp Leu Lys Arg Trp Thr Tyr Arg Ser Gly Phe Glu Arg Asp Asp 
    210                 215                 220                 


Leu Gly Ile Leu Glu Cys Pro Asp Leu Phe Arg Met Ser Val Asp Gly 
225                 230                 235                 240 


Asp Pro Ala Asn Thr Arg Trp Val Leu Ala Ala Gly Ala Asn Gly Ala 
                245                 250                 255     


Ser Lys Gly Met Thr Thr Gly Thr Val Tyr Trp Thr Gly Asp Trp Asp 
            260                 265                 270         


Gly Glu Arg Phe Thr Ala Asp Arg Asp Glu Pro Arg Trp Leu Asp Ser 
        275                 280                 285             


Gly Ala Asp Phe Tyr Ala Thr Val Thr Trp Asp Asp Pro Arg Gln Asp 
    290                 295                 300                 


Asp Ala Glu Arg Leu Ala Ser Arg Tyr Ala Ile Gly Trp Leu Asn Asn 
305                 310                 315                 320 


Trp Ala Tyr Ala Thr Lys Leu Pro Thr Asp Asp Trp His Gly Gly Ala 
                325                 330                 335     


Asp Ser Ile Val Arg Arg Ile Glu Leu Arg Ser Val Asp Gly Glu Pro 
            340                 345                 350         


Leu Leu Val Ser Gln Pro Val Asp Ala Leu Asp Glu Leu Ala Gly Asp 
        355                 360                 365             


Ala Glu Val Arg Ser Arg Val Arg Val Thr Ala Val Ser Gly Thr Thr 
    370                 375                 380                 


Leu Pro Gln Pro Glu Ser Asp Ala Tyr Arg Leu Arg Val Glu Leu Asp 
385                 390                 395                 400 


Ala Ala Ser Gly Ala Asn Glu Val Arg Phe Arg Val Lys Glu Gly Gly 
                405                 410                 415     


Gly His Phe Thr Thr Val Gly Tyr Asp Phe Val His Gly Val Ala Phe 
            420                 425                 430         


Val Ala Arg Asp Ala Asp Ala Ala Ala Asp Arg Met Pro Glu Ile Tyr 
        435                 440                 445             


Arg Glu Val Arg Thr Ala Pro Ala Pro Ala Arg Asp Gly Thr Val Thr 
    450                 455                 460                 


Leu Asp Val Ile Val Asp Ala Ala Ser Val Glu Val Phe Val Asn Asp 
465                 470                 475                 480 


Gly Glu Ala Val Leu Ser Asn Leu Ala Phe Gly Ala Pro Gly Ala Asn 
                485                 490                 495     


Gly Leu Ser Val Arg Ser Leu Gly Gly Asp Thr Glu Leu Arg Ser Phe 
            500                 505                 510         


Arg Leu Thr Pro Leu Ala Ile Ala Pro Ile Glu Arg His Tyr Gly Ala 
        515                 520                 525             


Gly Thr Pro Arg Arg Arg 
    530                 


<210>  6
<211>  551
<212>  PRT
<213>  Azotobacter chroococcum DSM 2286

<400>  6

Met Asn Arg Asn Pro Thr Ser Lys His Ala Ser Val Cys Ala Leu Ala 
1               5                   10                  15      


Ala Leu Leu Val Ala Ala Phe Val Ala Val Leu Val Leu Ala Pro Gly 
            20                  25                  30          


Pro Ala Thr Pro Ser Gly Pro Glu Pro Ser Ser Pro Pro Pro Ala Lys 
        35                  40                  45              


Ser Ser Glu Phe Ser Arg Pro Ala Asp Trp Ser Pro His Arg Pro Ala 
    50                  55                  60                  


Val His Leu Thr Pro Ala Lys His Trp Met Asn Asp Pro Gln Arg Pro 
65                  70                  75                  80  


Ile Leu Ile Asp Gly Val Trp His Tyr Tyr Tyr Leu Tyr Asn Ala Asp 
                85                  90                  95      


Tyr Pro Lys Gly Asn Gly Thr Glu Trp Tyr His Ala Thr Ser Thr Asp 
            100                 105                 110         


Leu Val His Trp Gln Asp His Gly Val Ala Ile Asp Lys Tyr Lys Asn 
        115                 120                 125             


Gly Leu Gly Asp Ile Glu Thr Gly Ser Ala Val Ile Asp Thr Glu Asn 
    130                 135                 140                 


Thr Ala Gly Phe Gly Ala Gly Ala Val Ile Ala Val Met Thr Gln Gln 
145                 150                 155                 160 


His Glu Gly Val Gln Arg Gln Ser Leu Phe Val Ser Thr Asp Gly Gly 
                165                 170                 175     


Tyr Arg Phe Lys Ala Tyr Asp Gly Asn Pro Val Met Asp Asn Pro Gly 
            180                 185                 190         


Ala Glu His Trp Arg Asp Pro Lys Ile Ile Trp Asp Asp Ala Arg Asp 
        195                 200                 205             


Glu Trp Leu Met Ala Leu Ala Glu Gly Asp Lys Ile Gly Phe Tyr Thr 
    210                 215                 220                 


Ser Pro Asp Leu Lys Arg Trp Thr Tyr Arg Ser Gly Phe Glu Arg Asp 
225                 230                 235                 240 


Asp Leu Gly Ile Leu Glu Cys Pro Asp Leu Phe Arg Met Ser Val Asp 
                245                 250                 255     


Gly Asp Pro Ala Asn Thr Arg Trp Val Leu Ala Ala Gly Ala Asn Gly 
            260                 265                 270         


Ala Ser Lys Gly Met Thr Thr Gly Thr Val Tyr Trp Thr Gly Asp Trp 
        275                 280                 285             


Asp Gly Glu Arg Phe Thr Ala Asp Arg Asp Glu Pro Arg Trp Leu Asp 
    290                 295                 300                 


Ser Gly Ala Asp Phe Tyr Ala Thr Val Thr Trp Asp Asp Pro Arg Gln 
305                 310                 315                 320 


Asp Asp Ala Glu Arg Leu Ala Ser Arg Tyr Ala Ile Gly Trp Leu Asn 
                325                 330                 335     


Asn Trp Ala Tyr Ala Thr Lys Leu Pro Thr Asp Asp Trp His Gly Gly 
            340                 345                 350         


Ala Asp Ser Ile Val Arg Arg Ile Glu Leu Arg Ser Val Asp Gly Glu 
        355                 360                 365             


Pro Leu Leu Val Ser Gln Pro Val Asp Ala Leu Asp Glu Leu Ala Gly 
    370                 375                 380                 


Asp Ala Glu Val Arg Ser Arg Val Arg Val Thr Ala Val Ser Gly Thr 
385                 390                 395                 400 


Thr Leu Pro Gln Pro Glu Ser Asp Ala Tyr Arg Leu Arg Val Glu Leu 
                405                 410                 415     


Asp Ala Ala Ser Gly Ala Asn Glu Val Arg Phe Arg Val Lys Glu Gly 
            420                 425                 430         


Gly Gly His Phe Thr Thr Val Gly Tyr Asp Phe Val His Gly Val Ala 
        435                 440                 445             


Phe Val Ala Arg Asp Ala Asp Ala Ala Ala Asp Arg Met Pro Glu Ile 
    450                 455                 460                 


Tyr Arg Glu Val Arg Thr Ala Pro Ala Pro Ala Arg Asp Gly Thr Val 
465                 470                 475                 480 


Thr Leu Asp Val Ile Val Asp Ala Ala Ser Val Glu Val Phe Val Asn 
                485                 490                 495     


Asp Gly Glu Ala Val Leu Ser Asn Leu Ala Phe Gly Ala Pro Gly Ala 
            500                 505                 510         


Asn Gly Leu Ser Val Arg Ser Leu Gly Gly Asp Thr Glu Leu Arg Ser 
        515                 520                 525             


Phe Arg Leu Thr Pro Leu Ala Ile Ala Pro Ile Glu Arg His Tyr Gly 
    530                 535                 540                 


Ala Gly Thr Pro Arg Arg Arg 
545                 550     


<210>  7
<211>  1605
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the modified endolevanase gene from 
       Azotobacter chroococcum DSM 2286

<400>  7
atggctagct ggagccaccc gcagttcgaa aaaggcgccc ccgctccggc gaccccgtca       60

ggccccgagc cgtcgagccc gccgccggcc aagtcctccg aattctcgcg gccggccgac      120

tggtcgcccc accggcccgc ggtgcacctc acccccgcca agcactggat gaacgatccc      180

cagcgcccga tcctgatcga cggcgtctgg cactactact acctctacaa cgccgactac      240

cccaagggga acggcaccga gtggtaccac gcgacctcga ccgacctcgt gcactggcag      300

gatcacggcg tcgcgatcga caagtacaag aacggcctcg gcgacatcga gaccggcagc      360

gccgtgatcg acaccgagaa cactgcgggc ttcggcgccg gcgcggtgat cgccgtcatg      420

acccagcagc acgagggcgt gcaacggcag tcgctcttcg tctcgaccga cggcggctac      480

cgcttcaagg cgtacgacgg caaccccgtc atggacaacc cgggcgccga gcactggcgt      540

gacccgaaga tcatctggga cgacgcccgc gacgagtggc tcatggcgct cgccgagggc      600

gacaagatcg gcttctacac ttcgcccgac ctgaagcgct ggacgtaccg atcgggcttc      660

gagcgcgacg acctcggcat cctcgagtgc cccgacctgt tccgcatgtc ggtcgacggt      720

gacccggcga acacgcgatg ggtgctcgcg gcaggcgcga acggtgccag caagggcatg      780

accacgggaa ccgtgtactg gaccggcgac tgggacggcg agcgcttcac cgcggatcgc      840

gacgaaccgc gatggctcga ctcgggcgcc gacttctacg ccaccgtgac gtgggacgac      900

ccgcgacagg acgacgccga acgcctcgcc tcccgctacg ccatcggctg gctcaacaac      960

tgggcctacg ccaccaagct gccgaccgac gactggcatg gcggcgccga ctcgatcgtg     1020

cgacgcatcg agctgcgatc ggtcgatggc gagccgctgc tcgtctcgca gcccgtcgac     1080

gcgctcgacg agctcgcggg cgacgccgag gtgcgttcca gggtacgagt gacggcagtc     1140

tcggggacga cgctccccca gccggaatcc gatgcctacc gactccgggt cgagctcgac     1200

gcggcctcgg gcgccaacga ggtgcgcttc cgcgtcaagg agggcggcgg gcacttcacg     1260

accgtcgggt acgacttcgt gcacggcgtc gccttcgtcg cgcgcgacgc ggatgctgcg     1320

gccgaccgga tgcccgagat ctaccgcgag gttcgtacgg ccccggcgcc ggcgcgcgac     1380

ggcaccgtca cccttgacgt catcgtcgat gcggcctcgg tcgaggtctt cgtgaacgac     1440

ggcgaggccg tgctctcgaa tctcgcgttc ggggcccccg gcgcgaacgg gctctcggtc     1500

aggtcgctcg gcggcgacac cgaactgcgc tccttccgac tcacgccgct cgcgattgcg     1560

ccgattgagc ggcattatgg agcaggcacc ccaaggcgac ggtga                     1605


<210>  8
<211>  1656
<212>  DNA
<213>  Azotobacter chroococcum DSM 2286

<400>  8
atgaaccgca atcccacctc gaagcacgca tcggtctgcg cgctcgccgc gctgctcgtc       60

gccgcgttcg tggcggtgct cgtgctcgcg cccggtccgg cgaccccgtc aggccccgag      120

ccgtcgagcc cgccgccggc caagtcctcc gaattctcgc ggccggccga ctggtcgccc      180

caccggcccg cggtgcacct cacccccgcc aagcactgga tgaacgatcc ccagcgcccg      240

atcctgatcg acggcgtctg gcactactac tacctctaca acgccgacta ccccaagggg      300

aacggcaccg agtggtacca cgcgacctcg accgacctcg tgcactggca ggatcacggc      360

gtcgcgatcg acaagtacaa gaacggcctc ggcgacatcg agaccggcag cgccgtgatc      420

gacaccgaga acactgcggg cttcggcgcc ggcgcggtga tcgccgtcat gacccagcag      480

cacgagggcg tgcaacggca gtcgctcttc gtctcgaccg acggcggcta ccgcttcaag      540

gcgtacgacg gcaaccccgt catggacaac ccgggcgccg agcactggcg tgacccgaag      600

atcatctggg acgacgcccg cgacgagtgg ctcatggcgc tcgccgaggg cgacaagatc      660

ggcttctaca cttcgcccga cctgaagcgc tggacgtacc gatcgggctt cgagcgcgac      720

gacctcggca tcctcgagtg ccccgacctg ttccgcatgt cggtcgacgg tgacccggcg      780

aacacgcgat gggtgctcgc ggcaggcgcg aacggtgcca gcaagggcat gaccacggga      840

accgtgtact ggaccggcga ctgggacggc gagcgcttca ccgcggatcg cgacgaaccg      900

cgatggctcg actcgggcgc cgacttctac gccaccgtga cgtgggacga cccgcgacag      960

gacgacgccg aacgcctcgc ctcccgctac gccatcggct ggctcaacaa ctgggcctac     1020

gccaccaagc tgccgaccga cgactggcat ggcggcgccg actcgatcgt gcgacgcatc     1080

gagctgcgat cggtcgatgg cgagccgctg ctcgtctcgc agcccgtcga cgcgctcgac     1140

gagctcgcgg gcgacgccga ggtgcgttcc agggtacgag tgacggcagt ctcggggacg     1200

acgctccccc agccggaatc cgatgcctac cgactccggg tcgagctcga cgcggcctcg     1260

ggcgccaacg aggtgcgctt ccgcgtcaag gagggcggcg ggcacttcac gaccgtcggg     1320

tacgacttcg tgcacggcgt cgccttcgtc gcgcgcgacg cggatgctgc ggccgaccgg     1380

atgcccgaga tctaccgcga ggttcgtacg gccccggcgc cggcgcgcga cggcaccgtc     1440

acccttgacg tcatcgtcga tgcggcctcg gtcgaggtct tcgtgaacga cggcgaggcc     1500

gtgctctcga atctcgcgtt cggggccccc ggcgcgaacg ggctctcggt caggtcgctc     1560

ggcggcgaca ccgaactgcg ctccttccga ctcacgccgc tcgcgattgc gccgattgag     1620

cggcattatg gagcaggcac cccaaggcga cggtga                               1656


<210>  9
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  p5_levS1417_for

<400>  9
attaccgcgg aaaatgctat ttccagccga a                                      31


<210>  10
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  p5_levS1417_rev

<400>  10
attactcgag tcaggcacga acgtcatagg                                        30


<210>  11
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  levS1417_EcoRV_for

<400>  11
attagatatc aggaggaaaa aaaaatgaat gctatttcca gc                          42


<210>  12
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  levS1417_AscI_rev

<400>  12
attaggcgcg cctcaggcac gaacgtcata                                        30


<210>  13
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  levB2286 _p5_f

<400>  13
atggtaggtc tcagcgcccc cgctccggcg accccg                                 36


<210>  14
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  levB2286 _p5_r

<400>  14
atggtaggtc tcatatcagt ctgcgggcgc cgctggc                                37


<210>  15
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  levS1417_Assembly_f

<400>  15
gacccgacac catcgaatgg attaattcct aatttttgtt gacactc                     47


<210>  16
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  levS1417_Assembly_r

<400>  16
attaggaatt aatcatctgg agatccgtga cgcagtag                               38


