                         SEQUENCE LISTING

<110>  Oncurius NV
 
<120>  COMBINATION TREATMENT OF MEDULLOBLASTOMA

<130>  PAT2538997PC00

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  348
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (1)..(348)
<223>  mouse variable part of heavy chain

<400>  1
cag atc cag ctg cag cag tct gga cct gag ctg gtg aag cct ggg gct         48
Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala           
1               5                   10                  15                

tca gtg aag ata tcc tgc aag gcc tct ggc tac acc ttc act gac tac         96
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr           
            20                  25                  30                    

tat ata aac tgg gtg aag ttg aag cct gga cag gga ctt gag tgg att        144
Tyr Ile Asn Trp Val Lys Leu Lys Pro Gly Gln Gly Leu Glu Trp Ile           
        35                  40                  45                        

gga tgg att tat cct gga agc ggt aat act aag tac aat gag aag ttc        192
Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe           
    50                  55                  60                            

aag ggc aag gcc aca ttg act ata gac aca tcc tcc agc aca gcc tac        240
Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr           
65                  70                  75                  80            

atg cag ctc agc agc ctg aca tct gag gac act gct gtc tat ttc tgt        288
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys           
                85                  90                  95                

gta aga gac agc cct ttc ttt gac tac tgg ggc caa ggc acc act ctc        336
Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu           
            100                 105                 110                   

aca gtc tcc tca                                                        348
Thr Val Ser Ser                                                           
        115                                                               


<210>  2
<211>  116
<212>  PRT
<213>  Mus musculus

<400>  2

Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Leu Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  3
<211>  336
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (1)..(336)
<223>  mouse variable part of light chain

<400>  3
gac att gtg ctg tca cag tct cca tcc tcc ctg gct gtg tca gca gga         48
Asp Ile Val Leu Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly           
1               5                   10                  15                

gag aag gtc act atg cgc tgc aaa tcc agt cag agt ctg ctc aac agt         96
Glu Lys Val Thr Met Arg Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser           
            20                  25                  30                    

gga atg cga aag agt ttc ttg gct tgg tac cag cag aaa cca ggg cag        144
Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln           
        35                  40                  45                        

tct cct aag ctg ctg atc tac tgg gca tcc act agg gaa tct ggg gtc        192
Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val           
    50                  55                  60                            

cct gat cgc ttc aca ggc agt gga tct ggg aca gat ttc act ctc acc        240
Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr           
65                  70                  75                  80            

atc agc agt gtg cag gct gaa gac ctg gca gtt tat tac tgc aag caa        288
Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Lys Gln           
                85                  90                  95                

tct tat cat cta ttc acg ttc ggc tcg ggg aca aag ttg gaa ata aaa        336
Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys           
            100                 105                 110                   


<210>  4
<211>  112
<212>  PRT
<213>  Mus musculus

<400>  4

Asp Ile Val Leu Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Arg Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  5
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDR1 heavy chain

<400>  5

Gly Tyr Thr Phe Thr Asp Tyr Tyr 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDR2 heavy chain

<400>  6

Ile Tyr Pro Gly Ser Gly Asn Thr 
1               5               


<210>  7
<211>  9
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  CDR3 heavy chain

<400>  7

Val Arg Asp Ser Pro Phe Phe Asp Tyr 
1               5                   


<210>  8
<211>  12
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  CDR1 light chain

<400>  8

Gln Ser Leu Leu Asn Ser Gly Met Arg Lys Ser Phe 
1               5                   10          


<210>  9
<211>  3
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  CDR2 light chain

<400>  9

Trp Ala Ser 
1           


<210>  10
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDR3 light chain

<400>  10

Lys Gln Ser Tyr His Leu Phe Thr 
1               5               


<210>  11
<211>  786
<212>  DNA
<213>  artificial sequence

<220>
<223>  murine-based ScFv


<220>
<221>  CDS
<222>  (1)..(786)

<220>
<221>  misc_feature
<222>  (1)..(348)
<223>  Heavy chain

<220>
<221>  misc_feature
<222>  (349)..(393)
<223>  linker

<220>
<221>  misc_feature
<222>  (394)..(729)
<223>  Light chain

<220>
<221>  misc_feature
<222>  (736)..(762)
<223>  HA-Tag

<220>
<221>  misc_feature
<222>  (769)..(786)
<223>  His-Tag

<400>  11
cag atc cag ctg cag cag tct gga cct gag ctg gtg aag cct ggg gct         48
Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala           
1               5                   10                  15                

tca gtg aag ata tcc tgc aag gcc tct ggc tac acc ttc act gac tac         96
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr           
            20                  25                  30                    

tat ata aac tgg gtg aag ttg aag cct gga cag gga ctt gag tgg att        144
Tyr Ile Asn Trp Val Lys Leu Lys Pro Gly Gln Gly Leu Glu Trp Ile           
        35                  40                  45                        

gga tgg att tat cct gga agc ggt aat act aag tac aat gag aag ttc        192
Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe           
    50                  55                  60                            

aag ggc aag gcc aca ttg act ata gac aca tcc tcc agc aca gcc tac        240
Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr           
65                  70                  75                  80            

atg cag ctc agc agc ctg aca tct gag gac act gct gtc tat ttc tgt        288
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys           
                85                  90                  95                

gta aga gac agc cct ttc ttt gac tac tgg ggc caa ggc acc act ctc        336
Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu           
            100                 105                 110                   

aca gtc tcc tca ggt ggt ggt ggt tct ggc ggc ggc ggc tcc ggt gga        384
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly           
        115                 120                 125                       

ggt ggt tct gac att gtg ctg tca cag tct cca tcc tcc ctg gct gtg        432
Gly Gly Ser Asp Ile Val Leu Ser Gln Ser Pro Ser Ser Leu Ala Val           
    130                 135                 140                           

tca gca gga gag aag gtc act atg cgc tgc aaa tcc agt cag agt ctg        480
Ser Ala Gly Glu Lys Val Thr Met Arg Cys Lys Ser Ser Gln Ser Leu           
145                 150                 155                 160           

ctc aac agt gga atg cga aag agt ttc ttg gct tgg tac cag cag aaa        528
Leu Asn Ser Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys           
                165                 170                 175               

cca ggg cag tct cct aag ctg ctg atc tac tgg gca tcc act agg gaa        576
Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu           
            180                 185                 190                   

tct ggg gtc cct gat cgc ttc aca ggc agt gga tct ggg aca gat ttc        624
Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe           
        195                 200                 205                       

act ctc acc atc agc agt gtg cag gct gaa gac ctg gca gtt tat tac        672
Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr           
    210                 215                 220                           

tgc aag caa tct tat cat cta ttc acg ttc ggc tcg ggg aca aag ttg        720
Cys Lys Gln Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu           
225                 230                 235                 240           

gaa ata aaa ggt tct tac cca tac gac gtc cca gac tac gct ggt tct        768
Glu Ile Lys Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ser           
                245                 250                 255               

cat cat cac cat cac cat                                                786
His His His His His His                                                   
            260                                                           


<210>  12
<211>  262
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  12

Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Leu Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Val Leu Ser Gln Ser Pro Ser Ser Leu Ala Val 
    130                 135                 140                 


Ser Ala Gly Glu Lys Val Thr Met Arg Cys Lys Ser Ser Gln Ser Leu 
145                 150                 155                 160 


Leu Asn Ser Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Lys Gln Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ser 
                245                 250                 255     


His His His His His His 
            260         


<210>  13
<211>  786
<212>  DNA
<213>  artificial sequence

<220>
<223>  ScFv Humanized


<220>
<221>  CDS
<222>  (1)..(786)

<220>
<221>  misc_feature
<222>  (1)..(348)
<223>  Heavy chain

<220>
<221>  misc_feature
<222>  (349)..(393)
<223>  linker

<220>
<221>  misc_feature
<222>  (394)..(729)
<223>  Light chain

<220>
<221>  misc_feature
<222>  (736)..(762)
<223>  HA-tag

<220>
<221>  misc_feature
<222>  (769)..(786)
<223>  His-tag

<400>  13
cag gtc cag ctg cag cag tct gga gcc gag ctg gtg aag cct ggg gct         48
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala           
1               5                   10                  15                

tca gtg aag ata tcc tgc aag gcc tct ggc tac acc ttc act gac tac         96
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr           
            20                  25                  30                    

tat ata aac tgg gtg aag ttg gcc cct gga cag gga ctt gag tgg att        144
Tyr Ile Asn Trp Val Lys Leu Ala Pro Gly Gln Gly Leu Glu Trp Ile           
        35                  40                  45                        

gga tgg att tat cct gga agc ggt aat act aag tac aat gag aag ttc        192
Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe           
    50                  55                  60                            

aag ggc aag gcc aca ttg act ata gac aca tcc tcc agc aca gcc tac        240
Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr           
65                  70                  75                  80            

atg cag ctc agc agc ctg aca tct gag gac act gct gtc tat ttc tgt        288
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys           
                85                  90                  95                

gta aga gac agc cct ttc ttt gac tac tgg ggc caa ggc acc ctg ctc        336
Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Leu           
            100                 105                 110                   

aca gtc tcc tca ggt ggt ggt ggt tct ggc ggc ggc ggc tcc ggt gga        384
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly           
        115                 120                 125                       

ggt ggt tct gac att gtg ctg acc cag tct cca gac tcc ctg gct gtg        432
Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val           
    130                 135                 140                           

tca ctg gga gag cgg gtc act atg aac tgc aaa tcc agt cag agt ctg        480
Ser Leu Gly Glu Arg Val Thr Met Asn Cys Lys Ser Ser Gln Ser Leu           
145                 150                 155                 160           

ctc aac agt gga atg cga aag agt ttc ttg gct tgg tac cag cag aaa        528
Leu Asn Ser Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys           
                165                 170                 175               

cca ggg cag tct cct aag ctg ctg atc tac tgg gca tcc act agg gaa        576
Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu           
            180                 185                 190                   

tct ggg gtc cct gat cgc ttc aca ggc agt gga tct ggg aca gat ttc        624
Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe           
        195                 200                 205                       

act ctc acc atc agc agt gtg cag gct gaa gac gtc gca gtt tat tac        672
Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr           
    210                 215                 220                           

tgc aag caa tct tat cat cta ttc acg ttc ggc tcg ggg aca aag ttg        720
Cys Lys Gln Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu           
225                 230                 235                 240           

gaa ata aaa ggt tct tac cca tac gac gtc cca gac tac gct ggt tct        768
Glu Ile Lys Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ser           
                245                 250                 255               

cat cat cac cat cac cat                                                786
His His His His His His                                                   
            260                                                           


<210>  14
<211>  262
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  14

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Leu Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Leu 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val 
    130                 135                 140                 


Ser Leu Gly Glu Arg Val Thr Met Asn Cys Lys Ser Ser Gln Ser Leu 
145                 150                 155                 160 


Leu Asn Ser Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Lys Gln Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ser 
                245                 250                 255     


His His His His His His 
            260         


<210>  15
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variable part of heavy chain

<400>  15

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Leu Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Val Arg Asp Ser Pro Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  16
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variable part of light chain

<400>  16

Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Met Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Met Arg Lys Ser Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr His Leu Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


