                               SEQUENCE LISTING

<110> WATERS TECHNOLOGIES CORPORATION
 
<120> COATINGS WITH IMMOBILIZED AFFINITY LIGANDS AND ENZYMES AND USE 
      THEREOF IN LIQUID CHROMATOGRAPHY ASSAYS

<130> W-4064-WO01 (102994.1014.2)

<140>
<141>

<150> 62/866,847
<151> 2019-06-26

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 139
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 1
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Ser Cys Ala Ala Ser Gly Gly Thr Ser Ile Arg Ile Gly 
            20                  25                  30          


Ser Ile Asn Ala Leu Ala Trp Tyr Arg Gln Ala Leu Gly Asn Gln Arg 
        35                  40                  45              


Glu Leu Val Ala Ala Val Thr Glu Gly Gly Ser Thr Asn Tyr Ala Asp 
    50                  55                  60                  


Phe Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Gln Ala Gln Asn Met 
65                  70                  75                  80  


Met Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Asn Ala Asp Lys Val Leu Tyr Ser Arg Gly Gly Tyr Tyr Ser 
            100                 105                 110         


Val Ala Asn Asp Leu Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


Gln Ala Pro Lys Val Asp Ala Lys Phe Asp Cys 
    130                 135                 


<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<220>
<221> source
<223> /note="Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide"

<400> 2
ggaggugcuc cgaaaggaac ucc                                               23


<210> 3
<211> 223
<212> PRT
<213> Sus sp.

<400> 3
Ile Val Gly Gly Tyr Thr Cys Ala Ala Asn Ser Ile Pro Tyr Gln Val 
1               5                   10                  15      


Ser Leu Asn Ser Gly Ser His Phe Cys Gly Gly Ser Leu Ile Asn Ser 
            20                  25                  30          


Gln Trp Val Val Ser Ala Ala His Cys Tyr Lys Ser Arg Ile Gln Val 
        35                  40                  45              


Arg Leu Gly Glu His Asn Ile Asp Val Leu Glu Gly Asn Glu Gln Phe 
    50                  55                  60                  


Ile Asn Ala Ala Lys Ile Ile Thr His Pro Asn Phe Asn Gly Asn Thr 
65                  70                  75                  80  


Leu Asp Asn Asp Ile Met Leu Ile Lys Leu Ser Ser Pro Ala Thr Leu 
                85                  90                  95      


Asn Ser Arg Val Ala Thr Val Ser Leu Pro Arg Ser Cys Ala Ala Ala 
            100                 105                 110         


Gly Thr Glu Cys Leu Ile Ser Gly Trp Gly Asn Thr Lys Ser Ser Gly 
        115                 120                 125             


Ser Ser Tyr Pro Ser Leu Leu Gln Cys Leu Lys Ala Pro Val Leu Ser 
    130                 135                 140                 


Asp Ser Ser Cys Lys Ser Ser Tyr Pro Gly Gln Ile Thr Gly Asn Met 
145                 150                 155                 160 


Ile Cys Val Gly Phe Leu Glu Gly Gly Lys Asp Ser Cys Gln Gly Asp 
                165                 170                 175     


Ser Gly Gly Pro Val Val Cys Asn Gly Gln Leu Gln Gly Ile Val Ser 
            180                 185                 190         


Trp Gly Tyr Gly Cys Ala Gln Lys Asn Lys Pro Gly Val Tyr Thr Lys 
        195                 200                 205             


Val Cys Asn Tyr Val Asn Trp Ile Gln Gln Thr Ile Ala Ala Asn 
    210                 215                 220             


<210> 4
<211> 630
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Beta-Glucuronidase sequence"

<400> 4
Gly Leu Gln Gly Gly Met Leu Tyr Pro Gln Glu Ser Pro Ser Arg Glu 
1               5                   10                  15      


Cys Lys Glu Leu Asp Gly Leu Trp Ser Phe Arg Ala Asp Phe Ser Asp 
            20                  25                  30          


Asn Arg Arg Arg Gly Phe Glu Glu Gln Trp Tyr Arg Arg Pro Leu Trp 
        35                  40                  45              


Glu Ser Gly Pro Thr Val Asp Met Pro Val Pro Ser Ser Phe Asn Asp 
    50                  55                  60                  


Ile Ser Gln Asp Trp Arg Leu Arg His Phe Val Gly Trp Val Trp Tyr 
65                  70                  75                  80  


Glu Arg Glu Val Ile Leu Pro Glu Arg Trp Thr Gln Asp Leu Arg Thr 
                85                  90                  95      


Arg Val Val Leu Arg Ile Gly Ser Ala His Ser Tyr Ala Ile Val Trp 
            100                 105                 110         


Val Asn Gly Val Asp Thr Leu Glu His Glu Gly Gly Tyr Leu Pro Phe 
        115                 120                 125             


Glu Ala Asp Ile Ser Asn Leu Val Gln Val Gly Pro Leu Pro Ser Arg 
    130                 135                 140                 


Leu Arg Ile Thr Ile Ala Ile Asn Asn Thr Leu Thr Pro Thr Thr Leu 
145                 150                 155                 160 


Pro Pro Gly Thr Ile Gln Tyr Leu Thr Asp Thr Ser Lys Tyr Pro Lys 
                165                 170                 175     


Gly Tyr Phe Val Gln Asn Thr Tyr Phe Asp Phe Phe Asn Tyr Ala Gly 
            180                 185                 190         


Leu Gln Arg Ser Val Leu Leu Tyr Thr Thr Pro Thr Thr Tyr Ile Asp 
        195                 200                 205             


Asp Ile Thr Val Thr Thr Ser Val Glu Gln Asp Ser Gly Leu Val Asn 
    210                 215                 220                 


Tyr Gln Ile Ser Val Lys Gly Ser Asn Leu Phe Lys Leu Glu Val Arg 
225                 230                 235                 240 


Leu Leu Asp Ala Glu Asn Lys Val Val Ala Asn Gly Thr Gly Thr Gln 
                245                 250                 255     


Gly Gln Leu Lys Val Pro Gly Val Ser Leu Trp Trp Pro Tyr Leu Met 
            260                 265                 270         


His Glu Arg Pro Ala Tyr Leu Tyr Ser Leu Glu Val Gln Leu Thr Ala 
        275                 280                 285             


Gln Thr Ser Leu Gly Pro Val Ser Asp Phe Tyr Thr Leu Pro Val Gly 
    290                 295                 300                 


Ile Arg Thr Val Ala Val Thr Lys Ser Gln Phe Leu Ile Asn Gly Lys 
305                 310                 315                 320 


Pro Phe Tyr Phe His Gly Val Asn Lys His Glu Asp Ala Asp Ile Arg 
                325                 330                 335     


Gly Lys Gly Phe Asp Trp Pro Leu Leu Val Lys Asp Phe Asn Leu Leu 
            340                 345                 350         


Arg Trp Leu Gly Ala Asn Ala Phe Arg Thr Ser His Tyr Pro Tyr Ala 
        355                 360                 365             


Glu Glu Val Met Gln Met Cys Asp Arg Tyr Gly Ile Val Val Ile Asp 
    370                 375                 380                 


Glu Cys Pro Gly Val Gly Leu Ala Leu Pro Gln Phe Phe Asn Asn Val 
385                 390                 395                 400 


Ser Leu His His His Met Gln Val Met Glu Glu Val Val Arg Arg Asp 
                405                 410                 415     


Lys Asn His Pro Ala Val Val Met Trp Ser Val Ala Asn Glu Pro Ala 
            420                 425                 430         


Ser His Leu Glu Ser Ala Gly Tyr Tyr Leu Lys Met Val Ile Ala His 
        435                 440                 445             


Thr Lys Ser Leu Asp Pro Ser Arg Pro Val Thr Phe Val Ser Asn Ser 
    450                 455                 460                 


Asn Tyr Ala Ala Asp Lys Gly Ala Pro Tyr Val Asp Val Ile Cys Leu 
465                 470                 475                 480 


Asn Ser Tyr Tyr Ser Trp Tyr His Asp Tyr Gly His Leu Glu Leu Ile 
                485                 490                 495     


Gln Leu Gln Leu Ala Thr Gln Phe Glu Asn Trp Tyr Lys Lys Tyr Gln 
            500                 505                 510         


Lys Pro Ile Ile Gln Ser Glu Tyr Gly Ala Glu Thr Ile Ala Gly Phe 
        515                 520                 525             


His Gln Asp Pro Pro Leu Met Phe Thr Glu Glu Tyr Gln Lys Ser Leu 
    530                 535                 540                 


Leu Glu Gln Tyr His Leu Gly Leu Asp Gln Lys Arg Arg Lys Tyr Val 
545                 550                 555                 560 


Val Gly Glu Leu Ile Trp Asn Phe Ala Asp Phe Met Thr Glu Gln Ser 
                565                 570                 575     


Pro Thr Arg Val Leu Gly Asn Lys Lys Gly Ile Phe Thr Arg Gln Arg 
            580                 585                 590         


Gln Pro Lys Ser Ala Ala Phe Leu Leu Arg Glu Arg Tyr Trp Lys Ile 
        595                 600                 605             


Ala Asn Glu Thr Arg Tyr Pro His Ser Val Ala Lys Ser Gln Cys Leu 
    610                 615                 620                 


Glu Asn Ser Leu Phe Thr 
625                 630 


