               SEQUENCE LISTING

<110> Niklovir AB

<120> NOVEL ANTIVIRAL THERAPIES

<130> P41903391PCT00

<150> SE1950810-0
<151> 2019-06-28

<160> 10

<170> BiSSAP 1.3.6

<210> 1
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer

<400> 1
gcggtcccac tcttcacag                                                 19


<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer

<400> 2
gcccagaggc tagtgttacc a                                              21


<210> 3
<211> 297
<212> PRT
<213> Ljungan virus 87-012


<220> 
<223> VP1

<400> 3
Gly Leu His Ser Trp Gly Ser Glu Met Asp Leu Val Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Asp Glu Ile Gln Asp Asn Glu Glu Ile Gln Thr Gln Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Glu Glu Ala Ala Thr Glu Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Ser Glu Gln Leu Asn Met Ser Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Phe Lys Lys His Lys Val Asn Ile Tyr Ala Ala Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Ala Val Gly Val 
                85                  90                  95      
Phe Asn Thr Glu Thr Ala Ala Ile Gln Lys Phe Asp Leu His Phe Pro 
            100                 105                 110         
Thr Ser Thr His Gly Ala Leu Ser Arg Phe Phe Cys Phe Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Ile His Ile Leu Asn Val Ser Thr Thr Asn Ala Phe Leu 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Asp Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Ala Thr Leu Glu Ser Asn Gly Thr Met Ile Ile Pro Pro Asn Glu Gln 
                165                 170                 175     
Met Thr Leu Cys Val Pro Tyr Tyr Ser Glu Val Pro Leu Arg Cys Val 
            180                 185                 190         
Lys Gly Ser Asp Arg Asn Ser Ala Gly Leu Gly Ser Leu Phe Thr Gln 
        195                 200                 205             
Ala Val Gly Arg Thr Ile Ser Asn Arg Val Gln Ile Phe Val Ser Phe 
    210                 215                 220                 
Arg Cys Pro Asn Phe Phe Phe Pro Leu Pro Ala Pro Arg Glu Ala Thr 
225                 230                 235                 240 
Ser Arg Ser Ile Leu Glu Arg Val Asp Glu Ala Asn Ala Glu Glu Leu 
                245                 250                 255     
Glu Ala Val Leu Glu Ala Arg Thr Pro Asp Ala Pro Leu Arg Leu Lys 
            260                 265                 270         
Phe Asn Pro Glu Asp Pro Leu Lys Gln Leu Arg Glu Ala Ala Lys Ala 
        275                 280                 285             
Tyr Phe Asn Ile Met His Ser Asp Glu 
    290                 295         

<210> 4
<211> 296
<212> PRT
<213> Ljungan virus M1146


<220> 
<223> VP1

<400> 4
Gly Met Ser Ser Trp Gly Ser Gln Met Asp Leu Ile Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Glu Glu Ile Gln Asp Ser Glu Glu Pro Glu Ser Ser Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Gln Glu Ala Ala Thr Ala Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Thr Glu Gln Val Asn Val Ala Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Phe Lys Gln His Lys Val Asp Ile Tyr Ser Thr Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Arg Phe Gln Ile 
                85                  90                  95      
Thr Asn Leu Asp Asn Ser Ser Ile Gly Arg Phe Thr Ile Pro Phe Pro 
            100                 105                 110         
Thr Thr Thr His Gly Ser Leu Ala Arg Phe Phe Ala Tyr Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Ile His Val Ile Asn Ile Ser Thr Thr Asn Ala Phe Leu 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Thr Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Gly Ser Leu Glu Ser Asn Gly Val Met Ile Ile Pro Pro Asn Glu Gln 
                165                 170                 175     
Met Thr Phe Cys Val Pro Tyr Tyr Ser Glu Ala Pro Leu Arg Thr Val 
            180                 185                 190         
Lys Gly Thr Gly Leu Ser Ala Gly Leu Gly Thr Phe Phe Tyr Gln Ala 
        195                 200                 205             
Val Gly Arg Thr Ile Gln Asn Arg Met Glu Ile Phe Val Ser Leu Arg 
    210                 215                 220                 
Cys Pro Asn Phe Phe Phe Pro Val Pro Ala Pro His Glu Ala Ser Ala 
225                 230                 235                 240 
Arg Ser Thr Leu Ala Arg Ile Asp Thr Ala Thr Glu Glu Glu Leu Asn 
                245                 250                 255     
Ala Ile Met Ser Ser Ala Glu Pro Asp Thr Pro Leu Arg Ile Gly Lys 
            260                 265                 270         
Pro Pro Glu Asp Pro Leu Lys Gln Leu Arg Glu Ala Ala Lys Thr Tyr 
        275                 280                 285             
Phe Lys Ile Tyr His Asp Lys Asp 
    290                 295     

<210> 5
<211> 296
<212> PRT
<213> Ljungan virus 64-7855


<220> 
<223> VP1

<400> 5
Gly Glu His Ser Trp Gly Ser Gln Met Asp Leu Leu Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Asp Glu Ile Gln Asp Ser Glu Glu Pro Glu Ser Ser Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Gln Asp Ala Ala Lys Ala Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Thr Glu Gln Ile Asn Met Ala Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Tyr Lys Gln His Asn Val Asp Ile Phe Ser Ala Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Arg His Thr Ile 
                85                  90                  95      
Thr Asn Ile Asp Asn Ala Asn Val Gly Arg Phe Thr Ile Pro Phe Pro 
            100                 105                 110         
Thr Thr Thr His Gly Ala Leu Ser Arg Phe Phe Ala Tyr Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Ile His Val Leu Asn Ile Ser Asn Thr Asn Ala Phe Met 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Asp Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Gly Ser Leu Glu Ser Asn Gly Val Met Ile Ile Pro Pro Gly Glu Gln 
                165                 170                 175     
Met Thr Met Cys Val Pro Phe Tyr Ser Glu Ala Pro Leu Arg Thr Val 
            180                 185                 190         
Lys Gly Thr Gly Ala Ser Ala Gly Leu Gly Val Phe Phe Tyr Gln Cys 
        195                 200                 205             
Val Gly Arg Ser Val Gln Asn Arg Leu Glu Leu Phe Ile Ser Leu Arg 
    210                 215                 220                 
Cys Pro Asn Phe Phe Phe Pro Val Pro Ala Pro His Glu Ala Ser Ser 
225                 230                 235                 240 
Arg Asp Val Leu Ala Arg Leu Asn Asp Ala Thr Glu Glu Glu Leu Glu 
                245                 250                 255     
Ala Val Leu Asn Ala Arg Asp Pro Asp Glu Pro Leu Arg Ile Gly Arg 
            260                 265                 270         
Ala Pro Glu Asp Pro Leu Lys Gln Leu Arg Glu Ala Ala Lys Ala Tyr 
        275                 280                 285             
Phe Lys Val Tyr His Asp Ile Glu 
    290                 295     

<210> 6
<211> 297
<212> PRT
<213> Ljungan virus 145SL


<220> 
<223> VP1

<400> 6
Gly Leu His Ser Trp Gly Ser Glu Met Asp Leu Leu Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Glu Glu Ile Gln Asp Met Glu Glu Pro Glu Ser Glu Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Glu Glu Ala Ala Thr Ala Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Ser Glu Gln Gln Asn Met Ala Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Phe Lys Gln His Arg Val Asp Ile Tyr Ser Ala Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Ala Val Gly Ile 
                85                  90                  95      
Phe Asn Val Thr Asn Ala Asn Ile Ser Lys Phe Asp Leu Asn Phe Pro 
            100                 105                 110         
Thr Thr Thr His Gly Ala Leu Cys Arg Phe Phe Cys Phe Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Leu His Ile Leu Asn Ile Ser Ser Ser Asn Ala Pro Val 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Asp Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Ala Thr Leu Glu Ser Asn Gly Val Ile Ile Ile Pro Pro Asn Glu Gln 
                165                 170                 175     
Met Thr Leu Cys Ile Pro Tyr Tyr Ser Glu Ala Pro Leu Arg Cys Val 
            180                 185                 190         
Lys Gly Pro His Ser Ala Gly Ala Gly Leu Gly Ser Ile Phe Thr Gln 
        195                 200                 205             
Cys Ile Gly Asn Ser Val Asn Asn Arg Ile Gln Ile Phe Val Ser Phe 
    210                 215                 220                 
Arg Cys Pro Asn Phe Phe Phe Pro Leu Pro Ala Pro His Glu Ala Ser 
225                 230                 235                 240 
Ser Arg Ser Ile Leu Gln Arg Ile Ser Thr Ala Ser Ala Asp Glu Leu 
                245                 250                 255     
Glu Ala Val Leu Asp Ala Lys Thr Pro Asp Ala Pro Val Arg Leu Cys 
            260                 265                 270         
Tyr Gln Pro Glu Asp Pro Leu Arg Gln Leu Arg Glu Ala Ala Lys Ala 
        275                 280                 285             
His Phe Asn Ile Met His Asn Asp Glu 
    290                 295         

<210> 7
<211> 297
<212> PRT
<213> Ljungan virus 174F


<220> 
<223> VP1

<400> 7
Gly Leu His Ser Trp Gly Ser Glu Met Asp Leu Val Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Glu Glu Ile Gln Asp Asn Glu Glu Ile Gln Thr Gln Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Glu Glu Ala Ala Thr Glu Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Ser Glu Gln Leu Asn Met Ser Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Phe Lys Lys His Lys Val Asn Ile Tyr Ala Ala Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Ala Val Gly Val 
                85                  90                  95      
Phe Asn Thr Glu Thr Ala Ala Ile Gln Lys Phe Asp Leu His Phe Pro 
            100                 105                 110         
Thr Ser Thr His Gly Ala Leu Ser Arg Phe Phe Cys Phe Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Ile His Ile Leu Asn Val Ser Thr Thr Asn Ala Phe Leu 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Asp Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Ala Thr Leu Glu Ser Asn Gly Thr Met Ile Ile Pro Pro Asn Glu Gln 
                165                 170                 175     
Met Thr Leu Cys Val Pro Tyr Tyr Ser Glu Val Pro Leu Arg Cys Val 
            180                 185                 190         
Lys Gly Ser Asp Arg Asn Ser Ala Gly Leu Gly Ser Leu Phe Thr Gln 
        195                 200                 205             
Ala Val Gly Arg Thr Ile Ser Asn Arg Val Gln Ile Phe Val Ser Phe 
    210                 215                 220                 
Arg Cys Pro Asn Phe Phe Phe Pro Leu Pro Ala Pro Arg Glu Ala Thr 
225                 230                 235                 240 
Ser Arg Ser Ile Leu Glu Arg Val Asp Glu Ala Asn Ala Glu Glu Leu 
                245                 250                 255     
Glu Ala Val Leu Glu Ala Arg Thr Pro Asp Ala Pro Leu Arg Leu Lys 
            260                 265                 270         
Phe Asn Pro Glu Asp Pro Leu Lys Gln Leu Arg Glu Ala Ala Lys Ala 
        275                 280                 285             
Tyr Phe Asn Ile Met His Ser Asp Glu 
    290                 295         

<210> 8
<211> 297
<212> PRT
<213> Ljungan virus


<220> 
<223> NP_705876.1

<400> 8
Gly Leu His Ser Trp Gly Ser Glu Met Asp Leu Val Asp Ser Leu Asp 
1               5                   10                  15      
Asn Pro Asp Glu Ile Gln Asp Asn Glu Glu Ile Gln Thr Gln Asn Val 
            20                  25                  30          
Glu Ala Ala Gln Gly Glu Glu Ala Ala Thr Glu Val Gly Leu Arg Ala 
        35                  40                  45              
Thr Glu Asn Asp Gly Ser Leu Ser Glu Gln Leu Asn Met Ser Gln Pro 
    50                  55                  60                  
Met Phe Leu Asn Phe Lys Lys His Lys Val Asn Ile Tyr Ala Ala Ser 
65                  70                  75                  80  
His Thr Lys Val Asp His Ile Phe Gly Arg Ala Trp Ala Val Gly Val 
                85                  90                  95      
Phe Asn Thr Glu Thr Ala Ala Ile Gln Lys Phe Asp Leu His Phe Pro 
            100                 105                 110         
Thr Ser Thr His Gly Ala Leu Ser Arg Phe Phe Cys Phe Trp Thr Gly 
        115                 120                 125             
Glu Leu Asn Ile His Ile Leu Asn Val Ser Thr Thr Asn Ala Phe Leu 
    130                 135                 140                 
Lys Val Ala His Thr Trp Phe Gly Thr Asp Ser Gly Ile Ala Arg Thr 
145                 150                 155                 160 
Ala Thr Leu Glu Ser Asn Gly Thr Met Ile Ile Pro Pro Asn Glu Gln 
                165                 170                 175     
Met Thr Leu Cys Val Pro Tyr Tyr Ser Glu Val Pro Leu Arg Cys Val 
            180                 185                 190         
Lys Gly Ser Asp Arg Asn Ser Ala Gly Leu Gly Ser Leu Phe Thr Gln 
        195                 200                 205             
Ala Val Gly Arg Thr Ile Ser Asn Arg Val Gln Ile Phe Val Ser Phe 
    210                 215                 220                 
Arg Cys Pro Asn Phe Phe Phe Pro Leu Pro Ala Pro Arg Glu Ala Thr 
225                 230                 235                 240 
Ser Arg Ser Ile Leu Glu Arg Val Asp Glu Ala Asn Ala Glu Glu Leu 
                245                 250                 255     
Glu Ala Val Leu Glu Ala Arg Thr Pro Asp Ala Pro Leu Arg Leu Lys 
            260                 265                 270         
Phe Asn Pro Glu Asp Pro Leu Lys Gln Leu Arg Glu Ala Ala Lys Ala 
        275                 280                 285             
Tyr Phe Asn Ile Met His Ser Asp Glu 
    290                 295         

<210> 9
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> sense primer

<400> 9
ttattaggat cggtttgtac tcatggggtt cagaa                                35


<210> 10
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> antisense primer

<400> 10
taataagcgg ccgcttatgg gttggtttcc acgtcaccac attggttcaa                50


