                         SEQUENCE LISTING

<110>  Dana-Farber Cancer Institute, Inc.
 
<120>  HCK DEGRADERS AND USES THEREOF

<130>  D0504.70177WO00

<140>  Not Yet Assigned
<141>  2020-06-24

<150>  US 62/865,780
<151>  2019-06-24

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  352
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Ser Gly Gly Arg Pro Ser Leu Cys Gln Phe Ile Leu Leu Gly 
1               5                   10                  15      


Thr Thr Ser Val Val Thr Ala Ala Leu Tyr Ser Val Tyr Arg Gln Lys 
            20                  25                  30          


Ala Arg Val Ser Gln Glu Leu Lys Gly Ala Lys Lys Val His Leu Gly 
        35                  40                  45              


Glu Asp Leu Lys Ser Ile Leu Ser Glu Ala Pro Gly Lys Cys Val Pro 
    50                  55                  60                  


Tyr Ala Val Ile Glu Gly Ala Val Arg Ser Val Lys Glu Thr Leu Asn 
65                  70                  75                  80  


Ser Gln Phe Val Glu Asn Cys Lys Gly Val Ile Gln Arg Leu Thr Leu 
                85                  90                  95      


Gln Glu His Lys Met Val Trp Asn Arg Thr Thr His Leu Trp Asn Asp 
            100                 105                 110         


Cys Ser Lys Ile Ile His Gln Arg Thr Asn Thr Val Pro Phe Asp Leu 
        115                 120                 125             


Val Pro His Glu Asp Gly Val Asp Val Ala Val Arg Val Leu Lys Pro 
    130                 135                 140                 


Leu Asp Ser Val Asp Leu Gly Leu Glu Thr Val Tyr Glu Lys Phe His 
145                 150                 155                 160 


Pro Ser Ile Gln Ser Phe Thr Asp Val Ile Gly His Tyr Ile Ser Gly 
                165                 170                 175     


Glu Arg Pro Lys Gly Ile Gln Glu Thr Glu Glu Met Leu Lys Val Gly 
            180                 185                 190         


Ala Thr Leu Thr Gly Val Gly Glu Leu Val Leu Asp Asn Asn Ser Val 
        195                 200                 205             


Arg Leu Gln Pro Pro Lys Gln Gly Met Gln Tyr Tyr Leu Ser Ser Gln 
    210                 215                 220                 


Asp Phe Asp Ser Leu Leu Gln Arg Gln Glu Ser Ser Val Arg Leu Trp 
225                 230                 235                 240 


Lys Val Leu Ala Leu Val Phe Gly Phe Ala Thr Cys Ala Thr Leu Phe 
                245                 250                 255     


Phe Ile Leu Arg Lys Gln Tyr Leu Gln Arg Gln Glu Arg Leu Arg Leu 
            260                 265                 270         


Lys Gln Met Gln Glu Glu Phe Gln Glu His Glu Ala Gln Leu Leu Ser 
        275                 280                 285             


Arg Ala Lys Pro Glu Asp Arg Glu Ser Leu Lys Ser Ala Cys Val Val 
    290                 295                 300                 


Cys Leu Ser Ser Phe Lys Ser Cys Val Phe Leu Glu Cys Gly His Val 
305                 310                 315                 320 


Cys Ser Cys Thr Glu Cys Tyr Arg Ala Leu Pro Glu Pro Lys Lys Cys 
                325                 330                 335     


Pro Ile Cys Arg Gln Ala Ile Thr Arg Val Ile Pro Pro Tyr Asn Ser 
            340                 345                 350         


<210>  2
<211>  949
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Glu Glu Gly Asn Asn Asn Glu Glu Val Ile His Leu Asn Asn Phe 
1               5                   10                  15      


His Cys His Arg Gly Gln Glu Trp Ile Asn Leu Arg Asp Gly Pro Ile 
            20                  25                  30          


Thr Ile Ser Asp Ser Ser Asp Glu Glu Arg Ile Pro Met Leu Val Thr 
        35                  40                  45              


Pro Ala Pro Gln Gln His Glu Glu Glu Asp Leu Asp Asp Asp Val Ile 
    50                  55                  60                  


Leu Thr Glu Thr Asn Lys Pro Gln Arg Ser Arg Pro Asn Leu Ile Lys 
65                  70                  75                  80  


Pro Ala Ala Gln Trp Gln Asp Leu Lys Arg Leu Gly Glu Glu Arg Pro 
                85                  90                  95      


Lys Lys Ser Arg Ala Ala Phe Glu Ser Asp Lys Ser Ser Tyr Phe Ser 
            100                 105                 110         


Val Cys Asn Asn Pro Leu Phe Asp Ser Gly Ala Gln Asp Asp Ser Glu 
        115                 120                 125             


Asp Asp Tyr Gly Glu Phe Leu Asp Leu Gly Pro Pro Gly Ile Ser Glu 
    130                 135                 140                 


Phe Thr Lys Pro Ser Gly Gln Thr Glu Arg Glu Pro Lys Pro Gly Pro 
145                 150                 155                 160 


Ser His Asn Gln Ala Ala Asn Asp Ile Val Asn Pro Arg Ser Glu Gln 
                165                 170                 175     


Lys Val Ile Ile Leu Glu Glu Gly Ser Leu Leu Tyr Thr Glu Ser Asp 
            180                 185                 190         


Pro Leu Glu Thr Gln Asn Gln Ser Ser Glu Asp Ser Glu Thr Glu Leu 
        195                 200                 205             


Leu Ser Asn Leu Gly Glu Ser Ala Ala Leu Ala Asp Asp Gln Ala Ile 
    210                 215                 220                 


Glu Glu Asp Cys Trp Leu Asp His Pro Tyr Phe Gln Ser Leu Asn Gln 
225                 230                 235                 240 


Gln Pro Arg Glu Ile Thr Asn Gln Val Val Pro Gln Glu Arg Gln Pro 
                245                 250                 255     


Glu Ala Glu Leu Gly Arg Leu Leu Phe Gln His Glu Phe Pro Gly Pro 
            260                 265                 270         


Ala Phe Pro Arg Pro Glu Pro Gln Gln Gly Gly Ile Ser Gly Pro Ser 
        275                 280                 285             


Ser Pro Gln Pro Ala His Pro Leu Gly Glu Phe Glu Asp Gln Gln Leu 
    290                 295                 300                 


Ala Ser Asp Asp Glu Glu Pro Gly Pro Ala Phe Pro Met Gln Glu Ser 
305                 310                 315                 320 


Gln Glu Pro Asn Leu Glu Asn Ile Trp Gly Gln Glu Ala Ala Glu Val 
                325                 330                 335     


Asp Gln Glu Leu Val Glu Leu Leu Val Lys Glu Thr Glu Ala Arg Phe 
            340                 345                 350         


Pro Asp Val Ala Asn Gly Phe Ile Glu Glu Ile Ile His Phe Lys Asn 
        355                 360                 365             


Tyr Tyr Asp Leu Asn Val Leu Cys Asn Phe Leu Leu Glu Asn Pro Asp 
    370                 375                 380                 


Tyr Pro Lys Arg Glu Asp Arg Ile Ile Ile Asn Pro Ser Ser Ser Leu 
385                 390                 395                 400 


Leu Ala Ser Gln Asp Glu Thr Lys Leu Pro Lys Ile Asp Phe Phe Asp 
                405                 410                 415     


Tyr Ser Lys Leu Thr Pro Leu Asp Gln Arg Cys Phe Ile Gln Ala Ala 
            420                 425                 430         


Asp Leu Leu Met Ala Asp Phe Lys Val Leu Ser Ser Gln Asp Ile Lys 
        435                 440                 445             


Trp Ala Leu His Glu Leu Lys Gly His Tyr Ala Ile Thr Arg Lys Ala 
    450                 455                 460                 


Leu Ser Asp Ala Ile Lys Lys Trp Gln Glu Leu Ser Pro Glu Thr Ser 
465                 470                 475                 480 


Gly Lys Arg Lys Lys Arg Lys Gln Met Asn Gln Tyr Ser Tyr Ile Asp 
                485                 490                 495     


Phe Lys Phe Glu Gln Gly Asp Ile Lys Ile Glu Lys Arg Met Phe Phe 
            500                 505                 510         


Leu Glu Asn Lys Arg Arg His Cys Arg Ser Tyr Asp Arg Arg Ala Leu 
        515                 520                 525             


Leu Pro Ala Val Gln Gln Glu Gln Glu Phe Tyr Glu Gln Lys Ile Lys 
    530                 535                 540                 


Glu Met Ala Glu His Glu Asp Phe Leu Leu Ala Leu Gln Met Asn Glu 
545                 550                 555                 560 


Glu Gln Tyr Gln Lys Asp Gly Gln Leu Ile Glu Cys Arg Cys Cys Tyr 
                565                 570                 575     


Gly Glu Phe Pro Phe Glu Glu Leu Thr Gln Cys Ala Asp Ala His Leu 
            580                 585                 590         


Phe Cys Lys Glu Cys Leu Ile Arg Tyr Ala Gln Glu Ala Val Phe Gly 
        595                 600                 605             


Ser Gly Lys Leu Glu Leu Ser Cys Met Glu Gly Ser Cys Thr Cys Ser 
    610                 615                 620                 


Phe Pro Thr Ser Glu Leu Glu Lys Val Leu Pro Gln Thr Ile Leu Tyr 
625                 630                 635                 640 


Lys Tyr Tyr Glu Arg Lys Ala Glu Glu Glu Val Ala Ala Ala Tyr Ala 
                645                 650                 655     


Asp Glu Leu Val Arg Cys Pro Ser Cys Ser Phe Pro Ala Leu Leu Asp 
            660                 665                 670         


Ser Asp Val Lys Arg Phe Ser Cys Pro Asn Pro His Cys Arg Lys Glu 
        675                 680                 685             


Thr Cys Arg Lys Cys Gln Gly Leu Trp Lys Glu His Asn Gly Leu Thr 
    690                 695                 700                 


Cys Glu Glu Leu Ala Glu Lys Asp Asp Ile Lys Tyr Arg Thr Ser Ile 
705                 710                 715                 720 


Glu Glu Lys Met Thr Ala Ala Arg Ile Arg Lys Cys His Lys Cys Gly 
                725                 730                 735     


Thr Gly Leu Ile Lys Ser Glu Gly Cys Asn Arg Met Ser Cys Arg Cys 
            740                 745                 750         


Gly Ala Gln Met Cys Tyr Leu Cys Arg Val Ser Ile Asn Gly Tyr Asp 
        755                 760                 765             


His Phe Cys Gln His Pro Arg Ser Pro Gly Ala Pro Cys Gln Glu Cys 
    770                 775                 780                 


Ser Arg Cys Ser Leu Trp Thr Asp Pro Thr Glu Asp Asp Glu Lys Leu 
785                 790                 795                 800 


Ile Glu Glu Ile Gln Lys Glu Ala Glu Glu Glu Gln Lys Arg Lys Asn 
                805                 810                 815     


Gly Glu Asn Thr Phe Lys Arg Ile Gly Pro Pro Leu Glu Lys Pro Val 
            820                 825                 830         


Glu Lys Val Gln Arg Val Glu Ala Leu Pro Arg Pro Val Pro Gln Asn 
        835                 840                 845             


Leu Pro Gln Pro Gln Met Pro Pro Tyr Ala Phe Ala His Pro Pro Phe 
    850                 855                 860                 


Pro Leu Pro Pro Val Arg Pro Val Phe Asn Asn Phe Pro Leu Asn Met 
865                 870                 875                 880 


Gly Pro Ile Pro Ala Pro Tyr Val Pro Pro Leu Pro Asn Val Arg Val 
                885                 890                 895     


Asn Tyr Asp Phe Gly Pro Ile His Met Pro Leu Glu His Asn Leu Pro 
            900                 905                 910         


Met His Phe Gly Pro Gln Pro Arg His Arg Phe Tyr Asp Phe Gly Pro 
        915                 920                 925             


Ile His Met Pro Leu Glu His Asn Leu Pro Met His Phe Gly Pro Gln 
    930                 935                 940                 


Pro Arg His Arg Phe 
945                 


<210>  3
<211>  866
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Glu Glu Gly Asn Asn Asn Glu Glu Val Ile His Leu Asn Asn Phe 
1               5                   10                  15      


His Cys His Arg Gly Gln Glu Trp Ile Asn Leu Arg Asp Gly Pro Ile 
            20                  25                  30          


Thr Ile Ser Asp Ser Ser Asp Glu Glu Arg Ile Pro Met Leu Val Thr 
        35                  40                  45              


Pro Ala Pro Gln Gln His Glu Glu Glu Asp Leu Asp Asp Asp Val Ile 
    50                  55                  60                  


Leu Thr Glu Asp Asp Ser Glu Asp Asp Tyr Gly Glu Phe Leu Asp Leu 
65                  70                  75                  80  


Gly Pro Pro Gly Ile Ser Glu Phe Thr Lys Pro Ser Gly Gln Thr Glu 
                85                  90                  95      


Arg Glu Pro Lys Pro Gly Pro Ser His Asn Gln Ala Ala Asn Asp Ile 
            100                 105                 110         


Val Asn Pro Arg Ser Glu Gln Lys Val Ile Ile Leu Glu Glu Gly Ser 
        115                 120                 125             


Leu Leu Tyr Thr Glu Ser Asp Pro Leu Glu Thr Gln Asn Gln Ser Ser 
    130                 135                 140                 


Glu Asp Ser Glu Thr Glu Leu Leu Ser Asn Leu Gly Glu Ser Ala Ala 
145                 150                 155                 160 


Leu Ala Asp Asp Gln Ala Ile Glu Glu Asp Cys Trp Leu Asp His Pro 
                165                 170                 175     


Tyr Phe Gln Ser Leu Asn Gln Gln Pro Arg Glu Ile Thr Asn Gln Val 
            180                 185                 190         


Val Pro Gln Glu Arg Gln Pro Glu Ala Glu Leu Gly Arg Leu Leu Phe 
        195                 200                 205             


Gln His Glu Phe Pro Gly Pro Ala Phe Pro Arg Pro Glu Pro Gln Gln 
    210                 215                 220                 


Gly Gly Ile Ser Gly Pro Ser Ser Pro Gln Pro Ala His Pro Leu Gly 
225                 230                 235                 240 


Glu Phe Glu Asp Gln Gln Leu Ala Ser Asp Asp Glu Glu Pro Gly Pro 
                245                 250                 255     


Ala Phe Pro Met Gln Glu Ser Gln Glu Pro Asn Leu Glu Asn Ile Trp 
            260                 265                 270         


Gly Gln Glu Ala Ala Glu Val Asp Gln Glu Leu Val Glu Leu Leu Val 
        275                 280                 285             


Lys Glu Thr Glu Ala Arg Phe Pro Asp Val Ala Asn Gly Phe Ile Glu 
    290                 295                 300                 


Glu Ile Ile His Phe Lys Asn Tyr Tyr Asp Leu Asn Val Leu Cys Asn 
305                 310                 315                 320 


Phe Leu Leu Glu Asn Pro Asp Tyr Pro Lys Arg Glu Asp Arg Ile Ile 
                325                 330                 335     


Ile Asn Pro Ser Ser Ser Leu Leu Ala Ser Gln Asp Glu Thr Lys Leu 
            340                 345                 350         


Pro Lys Ile Asp Phe Phe Asp Tyr Ser Lys Leu Thr Pro Leu Asp Gln 
        355                 360                 365             


Arg Cys Phe Ile Gln Ala Ala Asp Leu Leu Met Ala Asp Phe Lys Val 
    370                 375                 380                 


Leu Ser Ser Gln Asp Ile Lys Trp Ala Leu His Glu Leu Lys Gly His 
385                 390                 395                 400 


Tyr Ala Ile Thr Arg Lys Ala Leu Ser Asp Ala Ile Lys Lys Trp Gln 
                405                 410                 415     


Glu Leu Ser Pro Glu Thr Ser Gly Lys Arg Lys Lys Arg Lys Gln Met 
            420                 425                 430         


Asn Gln Tyr Ser Tyr Ile Asp Phe Lys Phe Glu Gln Gly Asp Ile Lys 
        435                 440                 445             


Ile Glu Lys Arg Met Phe Phe Leu Glu Asn Lys Arg Arg His Cys Arg 
    450                 455                 460                 


Ser Tyr Asp Arg Arg Ala Leu Leu Pro Ala Val Gln Gln Glu Gln Glu 
465                 470                 475                 480 


Phe Tyr Glu Gln Lys Ile Lys Glu Met Ala Glu His Glu Asp Phe Leu 
                485                 490                 495     


Leu Ala Leu Gln Met Asn Glu Glu Gln Tyr Gln Lys Asp Gly Gln Leu 
            500                 505                 510         


Ile Glu Cys Arg Cys Cys Tyr Gly Glu Phe Pro Phe Glu Glu Leu Thr 
        515                 520                 525             


Gln Cys Ala Asp Ala His Leu Phe Cys Lys Glu Cys Leu Ile Arg Tyr 
    530                 535                 540                 


Ala Gln Glu Ala Val Phe Gly Ser Gly Lys Leu Glu Leu Ser Cys Met 
545                 550                 555                 560 


Glu Gly Ser Cys Thr Cys Ser Phe Pro Thr Ser Glu Leu Glu Lys Val 
                565                 570                 575     


Leu Pro Gln Thr Ile Leu Tyr Lys Tyr Tyr Glu Arg Lys Ala Glu Glu 
            580                 585                 590         


Glu Val Ala Ala Ala Tyr Ala Asp Glu Leu Val Arg Cys Pro Ser Cys 
        595                 600                 605             


Ser Phe Pro Ala Leu Leu Asp Ser Asp Val Lys Arg Phe Ser Cys Pro 
    610                 615                 620                 


Asn Pro His Cys Arg Lys Glu Thr Cys Arg Lys Cys Gln Gly Leu Trp 
625                 630                 635                 640 


Lys Glu His Asn Gly Leu Thr Cys Glu Glu Leu Ala Glu Lys Asp Asp 
                645                 650                 655     


Ile Lys Tyr Arg Thr Ser Ile Glu Glu Lys Met Thr Ala Ala Arg Ile 
            660                 665                 670         


Arg Lys Cys His Lys Cys Gly Thr Gly Leu Ile Lys Ser Glu Gly Cys 
        675                 680                 685             


Asn Arg Met Ser Cys Arg Cys Gly Ala Gln Met Cys Tyr Leu Cys Arg 
    690                 695                 700                 


Val Ser Ile Asn Gly Tyr Asp His Phe Cys Gln His Pro Arg Ser Pro 
705                 710                 715                 720 


Gly Ala Pro Cys Gln Glu Cys Ser Arg Cys Ser Leu Trp Thr Asp Pro 
                725                 730                 735     


Thr Glu Asp Asp Glu Lys Leu Ile Glu Glu Ile Gln Lys Glu Ala Glu 
            740                 745                 750         


Glu Glu Gln Lys Arg Lys Asn Gly Glu Asn Thr Phe Lys Arg Ile Gly 
        755                 760                 765             


Pro Pro Leu Glu Lys Pro Val Glu Lys Val Gln Arg Val Glu Ala Leu 
    770                 775                 780                 


Pro Arg Pro Val Pro Gln Asn Leu Pro Gln Pro Gln Met Pro Pro Tyr 
785                 790                 795                 800 


Ala Phe Ala His Pro Pro Phe Pro Leu Pro Pro Val Arg Pro Val Phe 
                805                 810                 815     


Asn Asn Phe Pro Leu Asn Met Gly Pro Ile Pro Ala Pro Tyr Val Pro 
            820                 825                 830         


Pro Leu Pro Asn Val Arg Val Asn Tyr Asp Phe Gly Pro Ile His Met 
        835                 840                 845             


Pro Leu Glu His Asn Leu Pro Met His Phe Gly Pro Gln Pro Arg His 
    850                 855                 860                 


Arg Phe 
865     


