                         SEQUENCE LISTING

<110>  ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
 
<120>  INFLUENZA VIRUS NEURAMINIDASE AND USES THEREOF

<130>  6923-304-228

<140>
<141>

<150>  US 62/971,629
<151>  2020-02-07

<150>  US 62/867,077
<151>  2019-06-26

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  1391
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Del 25 Nucleotide (1391 bp)

<400>  1
agcaaaagca ggagtaaaga tgaatccaaa tcaaaagata ataacgattg gctctgtttc       60

tctcaccatt tccacaatat gcttcttcat gcaaattgcc attttgataa ctactgtaac      120

attgcatttc aagcaaatag tgtatttaac taacaccacc atagagaagg aaatatgccc      180

caaaccagca gaatacagaa attggtcaaa accgcaatgt ggcattacag gatttgcacc      240

tttctctaag gacaattcga ttaggctttc cgctggtggg gacatctggg tgacaagaga      300

accttatgtg tcatgcgatc ctgacaagtg ttatcaattt gcccttggac agggaacaac      360

actaaacaac gtgcattcaa ataacacagt acgtgatagg accccttatc ggactctatt      420

gatgaatgag ttgggtgttc ctttccatct ggggaccaag caagtgtgca tagcatggtc      480

cagctcaagt tgtcacgatg gaaaagcatg gctgcatgtt tgtataacgg gggatgataa      540

aaatgcaact gctagcttca tttacaatgg gaggcttgta gatagtgttg tttcatggtc      600

caaagatatt ctcaggaccc aggagtcaga atgcatttgt atcaatggaa cttgtacagt      660

agtaatgact gatggaagtg cttcaggaaa agctgatact aaaatactat tcattgagga      720

ggggaaaatc gttcatacta gcacattgtc aggaagtgct cagcatgtcg aagagtgctc      780

ttgctatcct cgatatcctg gtgtcagatg tgtctgcaga gacaactgga agggctccaa      840

tcggcccatc gtagatataa acataaagga tcatagcatt gtttccagtt atgtgtgttc      900

aggacttgtt ggagacacac ccagaaaaaa cgacagctcc agcagtagcc attgtttgga      960

tcctaacaat gaagaaggtg gtcatggagt gaaaggctgg gcctttgatg atggaaatga     1020

cgtgtggatg ggaagaacaa tcaacgagac gtcacgctta gggtatgaaa ccttcaaagt     1080

cattgaaggc tggtccaacc ctaagtccaa attgcagaca aataggcaag tcatagttga     1140

cagaggtgat aggtccggtt attctggtat tttctctgtt gaaggcaaaa gctgcatcaa     1200

tcggtgcttt tatgtggagt tgattagggg aagaaaagag gaaactgaag tcttgtggac     1260

ctcaaacagt attgttgtgt tttgtggcac ctcaggtaca tatggaacag gctcatggcc     1320

tgatggggcg gacctcaatc tcatgcctat ataagctttc gcaattttag aaaaaactcc     1380

ttgtttctac t                                                          1391


<210>  2
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Del 25 Amino Acid

<400>  2

Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 
1               5                   10                  15      


Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr 
            20                  25                  30          


Val Thr Leu His Phe Lys Gln Ile Val Tyr Leu Thr Asn Thr Thr Ile 
        35                  40                  45              


Glu Lys Glu Ile Cys Pro Lys Pro Ala Glu Tyr Arg Asn Trp Ser Lys 
    50                  55                  60                  


Pro Gln Cys Gly Ile Thr Gly Phe Ala Pro Phe Ser Lys Asp Asn Ser 
65                  70                  75                  80  


Ile Arg Leu Ser Ala Gly Gly Asp Ile Trp Val Thr Arg Glu Pro Tyr 
                85                  90                  95      


Val Ser Cys Asp Pro Asp Lys Cys Tyr Gln Phe Ala Leu Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Asn Asn Val His Ser Asn Asn Thr Val Arg Asp Arg Thr 
        115                 120                 125             


Pro Tyr Arg Thr Leu Leu Met Asn Glu Leu Gly Val Pro Phe His Leu 
    130                 135                 140                 


Gly Thr Lys Gln Val Cys Ile Ala Trp Ser Ser Ser Ser Cys His Asp 
145                 150                 155                 160 


Gly Lys Ala Trp Leu His Val Cys Ile Thr Gly Asp Asp Lys Asn Ala 
                165                 170                 175     


Thr Ala Ser Phe Ile Tyr Asn Gly Arg Leu Val Asp Ser Val Val Ser 
            180                 185                 190         


Trp Ser Lys Asp Ile Leu Arg Thr Gln Glu Ser Glu Cys Ile Cys Ile 
        195                 200                 205             


Asn Gly Thr Cys Thr Val Val Met Thr Asp Gly Ser Ala Ser Gly Lys 
    210                 215                 220                 


Ala Asp Thr Lys Ile Leu Phe Ile Glu Glu Gly Lys Ile Val His Thr 
225                 230                 235                 240 


Ser Thr Leu Ser Gly Ser Ala Gln His Val Glu Glu Cys Ser Cys Tyr 
                245                 250                 255     


Pro Arg Tyr Pro Gly Val Arg Cys Val Cys Arg Asp Asn Trp Lys Gly 
            260                 265                 270         


Ser Asn Arg Pro Ile Val Asp Ile Asn Ile Lys Asp His Ser Ile Val 
        275                 280                 285             


Ser Ser Tyr Val Cys Ser Gly Leu Val Gly Asp Thr Pro Arg Lys Asn 
    290                 295                 300                 


Asp Ser Ser Ser Ser Ser His Cys Leu Asp Pro Asn Asn Glu Glu Gly 
305                 310                 315                 320 


Gly His Gly Val Lys Gly Trp Ala Phe Asp Asp Gly Asn Asp Val Trp 
                325                 330                 335     


Met Gly Arg Thr Ile Asn Glu Thr Ser Arg Leu Gly Tyr Glu Thr Phe 
            340                 345                 350         


Lys Val Ile Glu Gly Trp Ser Asn Pro Lys Ser Lys Leu Gln Thr Asn 
        355                 360                 365             


Arg Gln Val Ile Val Asp Arg Gly Asp Arg Ser Gly Tyr Ser Gly Ile 
    370                 375                 380                 


Phe Ser Val Glu Gly Lys Ser Cys Ile Asn Arg Cys Phe Tyr Val Glu 
385                 390                 395                 400 


Leu Ile Arg Gly Arg Lys Glu Glu Thr Glu Val Leu Trp Thr Ser Asn 
                405                 410                 415     


Ser Ile Val Val Phe Cys Gly Thr Ser Gly Thr Tyr Gly Thr Gly Ser 
            420                 425                 430         


Trp Pro Asp Gly Ala Asp Leu Asn Leu Met Pro Ile 
        435                 440                 


<210>  3
<211>  1511
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Ins15 Nucleotide (1511 bp)

<400>  3
agcaaaagca ggagtaaaga tgaatccaaa tcaaaagata ataacgattg gctctgtttc       60

tctcaccatt tccacaatat gcttcttcat gcaaattgcc attttgataa ctactgtaac      120

attgcatttc aagcaatatg aattcaactc ccccccaaac aaccaagtga tgctgtgtga      180

accaacaata atagaaagaa acataacaga aatagtgtat ttaactaatc agacatatgt      240

taacatcagc aacaccaact ttgctgctgg aaacaccacc atagagaagg aaatatgccc      300

caaaccagca gaatacagaa attggtcaaa accgcaatgt ggcattacag gatttgcacc      360

tttctctaag gacaattcga ttaggctttc cgctggtggg gacatctggg tgacaagaga      420

accttatgtg tcatgcgatc ctgacaagtg ttatcaattt gcccttggac agggaacaac      480

actaaacaac gtgcattcaa ataacacagt acgtgatagg accccttatc ggactctatt      540

gatgaatgag ttgggtgttc ctttccatct ggggaccaag caagtgtgca tagcatggtc      600

cagctcaagt tgtcacgatg gaaaagcatg gctgcatgtt tgtataacgg gggatgataa      660

aaatgcaact gctagcttca tttacaatgg gaggcttgta gatagtgttg tttcatggtc      720

caaagatatt ctcaggaccc aggagtcaga atgcatttgt atcaatggaa cttgtacagt      780

agtaatgact gatggaagtg cttcaggaaa agctgatact aaaatactat tcattgagga      840

ggggaaaatc gttcatacta gcacattgtc aggaagtgct cagcatgtcg aagagtgctc      900

ttgctatcct cgatatcctg gtgtcagatg tgtctgcaga gacaactgga agggctccaa      960

tcggcccatc gtagatataa acataaagga tcatagcatt gtttccagtt atgtgtgttc     1020

aggacttgtt ggagacacac ccagaaaaaa cgacagctcc agcagtagcc attgtttgga     1080

tcctaacaat gaagaaggtg gtcatggagt gaaaggctgg gcctttgatg atggaaatga     1140

cgtgtggatg ggaagaacaa tcaacgagac gtcacgctta gggtatgaaa ccttcaaagt     1200

cattgaaggc tggtccaacc ctaagtccaa attgcagaca aataggcaag tcatagttga     1260

cagaggtgat aggtccggtt attctggtat tttctctgtt gaaggcaaaa gctgcatcaa     1320

tcggtgcttt tatgtggagt tgattagggg aagaaaagag gaaactgaag tcttgtggac     1380

ctcaaacagt attgttgtgt tttgtggcac ctcaggtaca tatggaacag gctcatggcc     1440

tgatggggcg gacctcaatc tcatgcctat ataagctttc gcaattttag aaaaaactcc     1500

ttgtttctac t                                                          1511


<210>  4
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Ins15 Amino Acid

<400>  4

Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 
1               5                   10                  15      


Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr 
            20                  25                  30          


Val Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn 
        35                  40                  45              


Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu 
    50                  55                  60                  


Ile Val Tyr Leu Thr Asn Gln Thr Tyr Val Asn Ile Ser Asn Thr Asn 
65                  70                  75                  80  


Phe Ala Ala Gly Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys Pro 
                85                  90                  95      


Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Gly Ile Thr Gly Phe 
            100                 105                 110         


Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly Asp 
        115                 120                 125             


Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys Cys 
    130                 135                 140                 


Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Val His Ser 
145                 150                 155                 160 


Asn Asn Thr Val Arg Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met Asn 
                165                 170                 175     


Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile Ala 
            180                 185                 190         


Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val Cys 
        195                 200                 205             


Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn Gly 
    210                 215                 220                 


Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Asp Ile Leu Arg Thr 
225                 230                 235                 240 


Gln Glu Ser Glu Cys Ile Cys Ile Asn Gly Thr Cys Thr Val Val Met 
                245                 250                 255     


Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe Ile 
            260                 265                 270         


Glu Glu Gly Lys Ile Val His Thr Ser Thr Leu Ser Gly Ser Ala Gln 
        275                 280                 285             


His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg Cys 
    290                 295                 300                 


Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp Ile 
305                 310                 315                 320 


Asn Ile Lys Asp His Ser Ile Val Ser Ser Tyr Val Cys Ser Gly Leu 
                325                 330                 335     


Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Ser His Cys 
            340                 345                 350         


Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp Ala 
        355                 360                 365             


Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu Thr 
    370                 375                 380                 


Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser Asn 
385                 390                 395                 400 


Pro Lys Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg Gly 
                405                 410                 415     


Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser Cys 
            420                 425                 430         


Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu Glu 
        435                 440                 445             


Thr Glu Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly Thr 
    450                 455                 460                 


Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Leu Asn 
465                 470                 475                 480 


Leu Met Pro Ile 
                


<210>  5
<211>  1466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 NA Nucleotide (1466 bp)

<400>  5
agcaaaagca ggagtaaaga tgaatccaaa tcaaaagata ataacgattg gctctgtttc       60

tctcaccatt tccacaatat gcttcttcat gcaaattgcc attttgataa ctactgtaac      120

attgcatttc aagcaatatg aattcaactc ccccccaaac aaccaagtga tgctgtgtga      180

accaacaata atagaaagaa acataacaga aatagtgtat ttaactaaca ccaccataga      240

gaaggaaata tgccccaaac cagcagaata cagaaattgg tcaaaaccgc aatgtggcat      300

tacaggattt gcacctttct ctaaggacaa ttcgattagg ctttccgctg gtggggacat      360

ctgggtgaca agagaacctt atgtgtcatg cgatcctgac aagtgttatc aatttgccct      420

tggacaggga acaacactaa acaacgtgca ttcaaataac acagtacgtg ataggacccc      480

ttatcggact ctattgatga atgagttggg tgttcctttc catctgggga ccaagcaagt      540

gtgcatagca tggtccagct caagttgtca cgatggaaaa gcatggctgc atgtttgtat      600

aacgggggat gataaaaatg caactgctag cttcatttac aatgggaggc ttgtagatag      660

tgttgtttca tggtccaaag atattctcag gacccaggag tcagaatgca tttgtatcaa      720

tggaacttgt acagtagtaa tgactgatgg aagtgcttca ggaaaagctg atactaaaat      780

actattcatt gaggagggga aaatcgttca tactagcaca ttgtcaggaa gtgctcagca      840

tgtcgaagag tgctcttgct atcctcgata tcctggtgtc agatgtgtct gcagagacaa      900

ctggaagggc tccaatcggc ccatcgtaga tataaacata aaggatcata gcattgtttc      960

cagttatgtg tgttcaggac ttgttggaga cacacccaga aaaaacgaca gctccagcag     1020

tagccattgt ttggatccta acaatgaaga aggtggtcat ggagtgaaag gctgggcctt     1080

tgatgatgga aatgacgtgt ggatgggaag aacaatcaac gagacgtcac gcttagggta     1140

tgaaaccttc aaagtcattg aaggctggtc caaccctaag tccaaattgc agacaaatag     1200

gcaagtcata gttgacagag gtgataggtc cggttattct ggtattttct ctgttgaagg     1260

caaaagctgc atcaatcggt gcttttatgt ggagttgatt aggggaagaa aagaggaaac     1320

tgaagtcttg tggacctcaa acagtattgt tgtgttttgt ggcacctcag gtacatatgg     1380

aacaggctca tggcctgatg gggcggacct caatctcatg cctatataag ctttcgcaat     1440

tttagaaaaa actccttgtt tctact                                          1466


<210>  6
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 NA Amino Acid

<400>  6

Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 
1               5                   10                  15      


Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr 
            20                  25                  30          


Val Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn 
        35                  40                  45              


Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu 
    50                  55                  60                  


Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys 
65                  70                  75                  80  


Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Gly Ile Thr Gly 
                85                  90                  95      


Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly 
            100                 105                 110         


Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys 
        115                 120                 125             


Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Val His 
    130                 135                 140                 


Ser Asn Asn Thr Val Arg Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met 
145                 150                 155                 160 


Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile 
                165                 170                 175     


Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 
            180                 185                 190         


Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn 
        195                 200                 205             


Gly Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Asp Ile Leu Arg 
    210                 215                 220                 


Thr Gln Glu Ser Glu Cys Ile Cys Ile Asn Gly Thr Cys Thr Val Val 
225                 230                 235                 240 


Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe 
                245                 250                 255     


Ile Glu Glu Gly Lys Ile Val His Thr Ser Thr Leu Ser Gly Ser Ala 
            260                 265                 270         


Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg 
        275                 280                 285             


Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp 
    290                 295                 300                 


Ile Asn Ile Lys Asp His Ser Ile Val Ser Ser Tyr Val Cys Ser Gly 
305                 310                 315                 320 


Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Ser His 
                325                 330                 335     


Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp 
            340                 345                 350         


Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu 
        355                 360                 365             


Thr Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser 
    370                 375                 380                 


Asn Pro Lys Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg 
385                 390                 395                 400 


Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser 
                405                 410                 415     


Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu 
            420                 425                 430         


Glu Thr Glu Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 
        435                 440                 445             


Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Leu 
    450                 455                 460                 


Asn Leu Met Pro Ile 
465                 


<210>  7
<211>  1458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins15 Nucleotide

<400>  7
agcgaaagca ggggtttaaa atgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatatgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatatgcaac caaaacatca      180

ttacctataa aaatagcacc tgggtaaatc agacatatgt taacatcagc aacaccaact      240

ttgctgctgg aaaggacaca acttcagtga tattaaccgg caattcatct ctttgtccca      300

tccgtgggtg ggctatatac agcaaagaca atagcataag aattggttcc aaaggagacg      360

tttttgtcat aagagagccc tttatttcat gttctcactt ggaatgcagg accttttttc      420

tgacccaagg tgccttactg aatgacaagc attcaaatgg gactgttaag gacagaagcc      480

cttatagggc cttaatgagc tgccctgtcg gtgaagctcc gtccccgtac aattcaagat      540

ttgaatcggt tgcttggtca gcaagtgcat gtcatgatgg catgggctgg ctaacaatcg      600

gaatttcagg tccagataat ggagcagtgg ctgtattaaa atacaacggc ataataactg      660

aaaccataaa aagttggagg aagaaaatat tgaggacaca agagtctgaa tgtgcctgtg      720

taaatggttc atgttttact ataatgactg atggcccgag tgatgggctg gcctcgtaca      780

aaattttcaa gatcgaaaag gggaaggtta ctaaatcaat agagttgaat gcacctaatt      840

ctcactatga ggaatgttcc tgttaccctg ataccggcaa agtgatgtgt gtgtgcagag      900

acaactggca tggttcgaac cggccatggg tgtctttcga tcaaaacctg gattatcaaa      960

taggatacat ctgcagtggg gttttcggtg acaacccgcg tcccgaagat ggaacaggca     1020

gctgtggtcc agtgtatgtt gatggagcaa acggagtaaa gggattttca tataggtatg     1080

gtaatggtgt ttggatagga aggaccaaaa gtcacagttc cagacatggg tttgagatga     1140

tttgggatcc taatggatgg acagagactg atagtaagtt ctctgttagg caagatgttg     1200

tggcaatgac tgattggtca gggtatagcg gaagtttcgt tcaacatcct gagctaacag     1260

ggctagactg tatgaggccg tgcttctggg ttgaattaat caggggacga cctaaagaaa     1320

aaacaatctg gactagtgcg agcagcattt ctttttgtgg cgtgaatagt gatactgtag     1380

attggtcttg gccagacggt gctgagttgc cattcagcat tgacaagtag tctgttcaaa     1440

aaactccttg tttctact                                                   1458


<210>  8
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins15 Amino Acid

<400>  8

Lys Met Asn Pro Asn Gln Lys Ile Thr Thr Ile Gly Ser Ile Cys Leu 
1               5                   10                  15      


Val Val Gly Leu Ile Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser 
            20                  25                  30          


Ile Trp Ile Ser His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly 
        35                  40                  45              


Ile Cys Asn Gln Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Asn 
    50                  55                  60                  


Gln Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Lys Asp 
65                  70                  75                  80  


Thr Thr Ser Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro Ile Arg 
                85                  90                  95      


Gly Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys 
            100                 105                 110         


Gly Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu 
        115                 120                 125             


Glu Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys 
    130                 135                 140                 


His Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met 
145                 150                 155                 160 


Ser Cys Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu 
                165                 170                 175     


Ser Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu 
            180                 185                 190         


Thr Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys 
        195                 200                 205             


Tyr Asn Gly Ile Ile Thr Glu Thr Ile Lys Ser Trp Arg Lys Lys Ile 
    210                 215                 220                 


Leu Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe 
225                 230                 235                 240 


Thr Ile Met Thr Asp Gly Pro Ser Asp Gly Leu Ala Ser Tyr Lys Ile 
                245                 250                 255     


Phe Lys Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala 
            260                 265                 270         


Pro Asn Ser His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys 
        275                 280                 285             


Val Met Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp 
    290                 295                 300                 


Val Ser Phe Asp Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser 
305                 310                 315                 320 


Gly Val Phe Gly Asp Asn Pro Arg Pro Glu Asp Gly Thr Gly Ser Cys 
                325                 330                 335     


Gly Pro Val Tyr Val Asp Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr 
            340                 345                 350         


Arg Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser His Ser Ser 
        355                 360                 365             


Arg His Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr 
    370                 375                 380                 


Asp Ser Lys Phe Ser Val Arg Gln Asp Val Val Ala Met Thr Asp Trp 
385                 390                 395                 400 


Ser Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu 
                405                 410                 415     


Asp Cys Met Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro 
            420                 425                 430         


Lys Glu Lys Thr Ile Trp Thr Ser Ala Ser Ser Ile Ser Phe Cys Gly 
        435                 440                 445             


Val Asn Ser Asp Thr Val Asp Trp Ser Trp Pro Asp Gly Ala Glu Leu 
    450                 455                 460                 


Pro Phe Ser Ile Asp Lys 
465                 470 


<210>  9
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins30 Nucleotide

<400>  9
agcgaaagca ggggtttaaa atgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatatgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatatgcaac caaaacatca      180

ttacctataa aaatagcacc tgggtaaatc agacatatgt taacatcagc aacaccaact      240

ttgctgctgg aaacacaaca gagatagtgt atctgaccaa caccaccata gagaagaagg      300

acacaacttc agtgatatta accggcaatt catctctttg tcccatccgt gggtgggcta      360

tatacagcaa agacaatagc ataagaattg gttccaaagg agacgttttt gtcataagag      420

agccctttat ttcatgttct cacttggaat gcaggacctt ttttctgacc caaggtgcct      480

tactgaatga caagcattca aatgggactg ttaaggacag aagcccttat agggccttaa      540

tgagctgccc tgtcggtgaa gctccgtccc cgtacaattc aagatttgaa tcggttgctt      600

ggtcagcaag tgcatgtcat gatggcatgg gctggctaac aatcggaatt tcaggtccag      660

ataatggagc agtggctgta ttaaaataca acggcataat aactgaaacc ataaaaagtt      720

ggaggaagaa aatattgagg acacaagagt ctgaatgtgc ctgtgtaaat ggttcatgtt      780

ttactataat gactgatggc ccgagtgatg ggctggcctc gtacaaaatt ttcaagatcg      840

aaaaggggaa ggttactaaa tcaatagagt tgaatgcacc taattctcac tatgaggaat      900

gttcctgtta ccctgatacc ggcaaagtga tgtgtgtgtg cagagacaac tggcatggtt      960

cgaaccggcc atgggtgtct ttcgatcaaa acctggatta tcaaatagga tacatctgca     1020

gtggggtttt cggtgacaac ccgcgtcccg aagatggaac aggcagctgt ggtccagtgt     1080

atgttgatgg agcaaacgga gtaaagggat tttcatatag gtatggtaat ggtgtttgga     1140

taggaaggac caaaagtcac agttccagac atgggtttga gatgatttgg gatcctaatg     1200

gatggacaga gactgatagt aagttctctg ttaggcaaga tgttgtggca atgactgatt     1260

ggtcagggta tagcggaagt ttcgttcaac atcctgagct aacagggcta gactgtatga     1320

ggccgtgctt ctgggttgaa ttaatcaggg gacgacctaa agaaaaaaca atctggacta     1380

gtgcgagcag catttctttt tgtggcgtga atagtgatac tgtagattgg tcttggccag     1440

acggtgctga gttgccattc agcattgaca agtagtctgt tcaaaaaact ccttgtttct     1500

act                                                                   1503


<210>  10
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins30 Amino Acid

<400>  10

Met Asn Pro Asn Gln Lys Ile Thr Thr Ile Gly Ser Ile Cys Leu Val 
1               5                   10                  15      


Val Gly Leu Ile Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 
            20                  25                  30          


Trp Ile Ser His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile 
        35                  40                  45              


Cys Asn Gln Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Asn Gln 
    50                  55                  60                  


Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Asn Thr Thr 
65                  70                  75                  80  


Glu Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Lys Asp Thr Thr 
                85                  90                  95      


Ser Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro Ile Arg Gly Trp 
            100                 105                 110         


Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly Asp 
        115                 120                 125             


Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu Cys 
    130                 135                 140                 


Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His Ser 
145                 150                 155                 160 


Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met Ser Cys 
                165                 170                 175     


Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser Val 
            180                 185                 190         


Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr Ile 
        195                 200                 205             


Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr Asn 
    210                 215                 220                 


Gly Ile Ile Thr Glu Thr Ile Lys Ser Trp Arg Lys Lys Ile Leu Arg 
225                 230                 235                 240 


Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr Ile 
                245                 250                 255     


Met Thr Asp Gly Pro Ser Asp Gly Leu Ala Ser Tyr Lys Ile Phe Lys 
            260                 265                 270         


Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala Pro Asn 
        275                 280                 285             


Ser His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val Met 
    290                 295                 300                 


Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val Ser 
305                 310                 315                 320 


Phe Asp Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly Val 
                325                 330                 335     


Phe Gly Asp Asn Pro Arg Pro Glu Asp Gly Thr Gly Ser Cys Gly Pro 
            340                 345                 350         


Val Tyr Val Asp Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Arg Tyr 
        355                 360                 365             


Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser His Ser Ser Arg His 
    370                 375                 380                 


Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp Ser 
385                 390                 395                 400 


Lys Phe Ser Val Arg Gln Asp Val Val Ala Met Thr Asp Trp Ser Gly 
                405                 410                 415     


Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp Cys 
            420                 425                 430         


Met Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys Glu 
        435                 440                 445             


Lys Thr Ile Trp Thr Ser Ala Ser Ser Ile Ser Phe Cys Gly Val Asn 
    450                 455                 460                 


Ser Asp Thr Val Asp Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro Phe 
465                 470                 475                 480 


Ser Ile Asp Lys 
                


<210>  11
<211>  1413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 N1-wt Nucleotide

<400>  11
agcgaaagca ggggtttaaa atgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatatgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatatgcaac caaaacatca      180

ttacctataa aaatagcacc tgggtaaagg acacaacttc agtgatatta accggcaatt      240

catctctttg tcccatccgt gggtgggcta tatacagcaa agacaatagc ataagaattg      300

gttccaaagg agacgttttt gtcataagag agccctttat ttcatgttct cacttggaat      360

gcaggacctt ttttctgacc caaggtgcct tactgaatga caagcattca aatgggactg      420

ttaaggacag aagcccttat agggccttaa tgagctgccc tgtcggtgaa gctccgtccc      480

cgtacaattc aagatttgaa tcggttgctt ggtcagcaag tgcatgtcat gatggcatgg      540

gctggctaac aatcggaatt tcaggtccag ataatggagc agtggctgta ttaaaataca      600

acggcataat aactgaaacc ataaaaagtt ggaggaagaa aatattgagg acacaagagt      660

ctgaatgtgc ctgtgtaaat ggttcatgtt ttactataat gactgatggc ccgagtgatg      720

ggctggcctc gtacaaaatt ttcaagatcg aaaaggggaa ggttactaaa tcaatagagt      780

tgaatgcacc taattctcac tatgaggaat gttcctgtta ccctgatacc ggcaaagtga      840

tgtgtgtgtg cagagacaac tggcatggtt cgaaccggcc atgggtgtct ttcgatcaaa      900

acctggatta tcaaatagga tacatctgca gtggggtttt cggtgacaac ccgcgtcccg      960

aagatggaac aggcagctgt ggtccagtgt atgttgatgg agcaaacgga gtaaagggat     1020

tttcatatag gtatggtaat ggtgtttgga taggaaggac caaaagtcac agttccagac     1080

atgggtttga gatgatttgg gatcctaatg gatggacaga gactgatagt aagttctctg     1140

ttaggcaaga tgttgtggca atgactgatt ggtcagggta tagcggaagt ttcgttcaac     1200

atcctgagct aacagggcta gactgtatga ggccgtgctt ctgggttgaa ttaatcaggg     1260

gacgacctaa agaaaaaaca atctggacta gtgcgagcag catttctttt tgtggcgtga     1320

atagtgatac tgtagattgg tcttggccag acggtgctga gttgccattc agcattgaca     1380

agtagtctgt tcaaaaaact ccttgtttct act                                  1413


<210>  12
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR 8 N1-wt Amino Acid

<400>  12

Met Asn Pro Asn Gln Lys Ile Thr Thr Ile Gly Ser Ile Cys Leu Val 
1               5                   10                  15      


Val Gly Leu Ile Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 
            20                  25                  30          


Trp Ile Ser His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile 
        35                  40                  45              


Cys Asn Gln Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Lys Asp 
    50                  55                  60                  


Thr Thr Ser Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro Ile Arg 
65                  70                  75                  80  


Gly Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys 
                85                  90                  95      


Gly Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu 
            100                 105                 110         


Glu Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys 
        115                 120                 125             


His Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met 
    130                 135                 140                 


Ser Cys Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu 
145                 150                 155                 160 


Ser Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu 
                165                 170                 175     


Thr Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys 
            180                 185                 190         


Tyr Asn Gly Ile Ile Thr Glu Thr Ile Lys Ser Trp Arg Lys Lys Ile 
        195                 200                 205             


Leu Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe 
    210                 215                 220                 


Thr Ile Met Thr Asp Gly Pro Ser Asp Gly Leu Ala Ser Tyr Lys Ile 
225                 230                 235                 240 


Phe Lys Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala 
                245                 250                 255     


Pro Asn Ser His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys 
            260                 265                 270         


Val Met Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp 
        275                 280                 285             


Val Ser Phe Asp Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser 
    290                 295                 300                 


Gly Val Phe Gly Asp Asn Pro Arg Pro Glu Asp Gly Thr Gly Ser Cys 
305                 310                 315                 320 


Gly Pro Val Tyr Val Asp Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr 
                325                 330                 335     


Arg Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser His Ser Ser 
            340                 345                 350         


Arg His Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr 
        355                 360                 365             


Asp Ser Lys Phe Ser Val Arg Gln Asp Val Val Ala Met Thr Asp Trp 
    370                 375                 380                 


Ser Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu 
385                 390                 395                 400 


Asp Cys Met Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro 
                405                 410                 415     


Lys Glu Lys Thr Ile Trp Thr Ser Ala Ser Ser Ile Ser Phe Cys Gly 
            420                 425                 430         


Val Asn Ser Asp Thr Val Asp Trp Ser Trp Pro Asp Gly Ala Glu Leu 
        435                 440                 445             


Pro Phe Ser Ile Asp Lys 
    450                 


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing Primer pDZ_forward

<400>  13
tacagctcct gggcaacgtg ctgg                                              24


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing Primer pDZ_reverse

<400>  14
aggtgtccgt gtcgcgcgtc gcc                                               23


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PR8_NA_forward

<400>  15
cgaaagcagg ggtttaaaat g                                                 21


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PR8_NA_reverse

<400>  16
tttttgaaca gactacttgt caatg                                             25


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PR8_HA_forward

<400>  17
ccgaagttgg gggggagcaa aagcagggga aaataa                                 36


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PR8_HA_reverse

<400>  18
ggccgccggg ttattagtag aaacaagggt gttttt                                 36


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HK14_NA_forward

<400>  19
gggagcaaaa gcaggagtaa agatg                                             25


<210>  20
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HK14_NA_reverse

<400>  20
ttattagtag aaacaaggag ttttttctaa aattgcg                                37


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HK14_HA_forward

<400>  21
gggagcaaaa gcaggggata attc                                              24


<210>  22
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HK14_HA_reverse

<400>  22
gggttattag tagaaacaag ggtgttttta attaatg                                37


<210>  23
<211>  2092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 swap virus - NA-HAmut-NA

<400>  23
agcgaaagca ggggtttaaa ttgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatttgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatttgcaac caagctagca      180

tgaaagcgaa tttgttagtt ttactgtccg cgttggcggc cgcggacgca gacacaatat      240

gtataggcta ccatgcgaac aattcaaccg acactgttga cacagtactc gagaagaatg      300

tgacagtgac acactctgtt aacctgctcg aagacagcca caacggaaaa ctatgtagat      360

taaaaggaat agccccacta caattgggga aatgtaacat cgccggatgg ctcttgggaa      420

acccagaatg cgacccactg cttccagtga gatcatggtc ctacattgta gaaacaccaa      480

actctgagaa tggaatatgt tatccaggag atttcatcga ctatgaggag ctgagggagc      540

aattgagctc agtgtcatca ttcgaaagat tcgaaatatt tcccaaagaa agctcatggc      600

ccaaccacaa cacaaacgga gtaacggcag catgctccca tgaggggaaa agcagttttt      660

acagaaattt gctatggctg acggagaagg agggctcata cccaaagctg aaaaattctt      720

atgtgaacaa aaaagggaaa gaagtccttg tactgtgggg tattcatcac ccgcctaaca      780

gtaaggaaca acagaatatc tatcagaatg aaaatgctta tgtctctgta gtgacttcaa      840

attataacag gagatttacc ccggaaatag cagaaagacc caaagtaaga gatcaagctg      900

ggaggatgaa ctattactgg accttgctaa aacccggaga cacaataata tttgaggcaa      960

atggaaatct aatagcacca atgtatgctt tcgcactgag tagaggcttt gggtccggca     1020

tcatcacctc aaacgcatca atgcatgagt gtaacacgaa gtgtcaaaca cccctgggag     1080

ctataaacag cagtctccct taccagaata tacacccagt cacaatagga gagtgcccaa     1140

aatacgtcag gagtgccaaa ttgaggatgg ttacaggact aaggaacact ccgtccattc     1200

aatccagagg tctatttgga gccattgccg gttttattga agggggatgg actggaatga     1260

tagatggatg gtatggttat catcatcaga atgaacaggg atcaggctat gcagcggatc     1320

aaaaaagcac acaaaatgcc attaacggga ttacaaacaa ggtgaacact gttatcgaga     1380

aaatgaacat tcaattcaca gctgtgggta aagaattcaa caaattagaa aaaaggatgg     1440

aaaatttaaa taaaaaagtt gatgatggat ttctggacat ttggacatat aatgcagaat     1500

tgttagttct actggaaaat gaaaggactc tggatttcca tgactcaaat gtgaagaatc     1560

tgtatgagaa agtaaaaagc caattaaaga ataatgccaa agaaatcgga aatggatgtt     1620

ttgagttcta ccacaagtgt gacaatgaat gcatggaaag tgtaagaaat gggacttatg     1680

attatcccaa atattcagaa gagtcaaagt tgaacaggga aaaggtagat ggagtgaaat     1740

tggaatcaat ggggatctat cagattctgg cgatctactc aactgtcgct tccagcttag     1800

tattgctagt tagtttagga gcgatttcct tttggatgtg cagcaacggg agcctacaat     1860

gtcggatttg tatttgactc gagtgagcta acagggctag actgtatgag gccgtgcttc     1920

tgggttgaat taatcagggg acgacctaaa gaaaaaacaa tctggactag tgcgagcagc     1980

atttcttttt gtggcgtgaa tagtgatact gtagattggt cttggccaga cggtgctgag     2040

ttgccattca gcattgacaa gtagtctgtt caaaaaactc cttgtttcta ct             2092


<210>  24
<211>  1626
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 swap virus - HA-NAmut-HA

<400>  24
agcaaaagca ggggaaaata aaaacaacca aattgaaggc aaacctactg gtcctgttaa       60

gtgcacttgc agctgcagtt gcagacacaa tttgtatagg ctagcatgaa cccgaaccaa      120

aagatcacga ctatcgggag catttgctta gtggttgggt tgatcagcct aatattgcaa      180

atagggaata taatctcaat atggattagc cattcaattc aaactggaag tcaaaaccat      240

actggaatat gcaaccaaaa catcattacc tataaaaata gcacctgggt aaaggacaca      300

acttcagtga tattaaccgg caattcatct ctttgtccca tccgtgggtg ggctatatac      360

agcaaagaca atagcataag aattggttcc aaaggagacg tttttgtcat aagagagccc      420

tttatttcat gttctcactt ggaatgcagg accttttttc tgacccaagg tgccttactg      480

aatgacaagc attcaaatgg gactgttaag gacagaagcc cttatagggc cttaatgagc      540

tgccctgtcg gtgaagctcc gtccccgtac aattcaagat ttgaatcggt tgcttggtca      600

gcaagtgcat gtcatgatgg catgggctgg ctaacaatcg gaatttcagg tccagataat      660

ggagcagtgg ctgtattaaa atacaacggc ataataactg aaaccataaa aagttggagg      720

aagaaaatat tgaggacaca agagtctgaa tgtgcctgtg taaatggttc atgttttact      780

ataatgactg atggcccgag tgatgggctg gcctcgtaca aaattttcaa gatcgaaaag      840

gggaaggtta ctaaatcaat agagttgaat gcacctaatt ctcactatga ggaatgttcc      900

tgttaccctg ataccggcaa agtgatgtgt gtgtgcagag acaactggca tggttcgaac      960

cggccatggg tgtctttcga tcaaaacctg gattatcaaa taggatacat ctgcagtggg     1020

gttttcggtg acaacccgcg tcccgaagat ggaacaggca gctgtggtcc agtgtatgtt     1080

gatggagcaa acggagtaaa gggattttca tataggtatg gtaatggtgt ttggatagga     1140

aggaccaaaa gtcacagttc cagacatggg tttgagatga tttgggatcc taatggatgg     1200

acagagactg atagtaagtt ctctgttagg caagatgttg tggcaatgac tgattggtca     1260

gggtatagcg gaagtttcgt tcaacatcct gagctaacag ggctagactg tatgaggccg     1320

tgcttctggg ttgaattaat caggggacga cctaaagaaa aaacaatctg gactagtgcg     1380

agcagcattt ctttttgtgg cgtgaatagt gacaccgtag actggagctg gccggatggc     1440

gccgaactac cgttttctat cgataaatag ctcgagatct actcaactgt cgccagttca     1500

ctggtgcttt tggtctccct gggggcaatc agtttctgga tgtgttctaa tggatctttg     1560

cagtgcagaa tatgcatctg agattagaat ttcagaaata tgaggaaaaa cacccttgtt     1620

tctact                                                                1626


<210>  25
<211>  2095
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 swap virus - NA - HK14 HA ORF - NA

<400>  25
agcgaaagca ggggtttaaa ttgaatccaa atcagaaaat aacaaccatt ggatcaatct       60

gtctggtagt cggactaatt agcctaatat tgcaaatagg gaatataatc tcaatttgga      120

ttagccattc aattcaaact ggaagtcaaa accatactgg aatttgcaac caagctagca      180

tgaagactat cattgctttg agctacattc tatgtctggt tttcgctcaa aaaattcctg      240

gaaatgacaa tagcacggca acgctgtgcc ttgggcacca tgcagtacca aacggaacga      300

tagtgaaaac aatcacgaat gaccgaattg aagttactaa tgctactgag ctggttcaga      360

attcctcaat aggtgaaata tgcgacagtc ctcatcagat ccttgatgga gaaaactgca      420

cactaataga tgctctattg ggagaccctc agtgtgatgg ctttcaaaat aagaaatggg      480

acctttttgt tgaacgaagc aaagcctaca gcagctgtta cccttatgat gtgccggatt      540

atgcctccct taggtcacta gttgcctcat ccggcacact ggagtttaac aatgaaagct      600

tcaattggac tggagtcact caaaacggaa caagttctgc ttgcataagg agatctagta      660

gtagtttctt tagtagatta aattggttga cccacttaaa ctacaaatac ccagcattga      720

acgtgactat gccaaacaat gaacaatttg acaaattgta catttggggg gttcaccacc      780

cgggtacgga caaggaccaa atcttcccgt atgctcaatc atcaggaaga atcacagtat      840

ctaccaaaag aagccaacaa gctgtaatcc caaatatcgg atctagaccc agaataagga      900

atatccctag cagaataagc atctattgga caatagtaaa accgggagac atacttttga      960

ttaacagcac agggaatcta attgctccta ggggttactt caaaatacga agtgggaaaa     1020

gctcaataat gagatcagat gcacccattg gcaaatgcaa gtctgaatgc atcactccaa     1080

atggaagcat tcccaatgac aaaccattcc aaaatgtaaa caggatcaca tacggggcct     1140

gtcccagata tgttaagcat agcactctga aattggcaac aggaatgcga aatgtaccag     1200

agaaacaaac tagaggcata tttggcgcaa tagcgggttt catagaaaat ggttgggagg     1260

gaatggtgga tggttggtac ggtttcaggc atcaaaattc tgagggaaga ggacaagcag     1320

cagatctcaa aagcactcaa gcagcaatcg atcaaatcaa tgggaagctg aatcgattga     1380

tcgggaaaac caacgagaaa ttccatcaga ttgaaaaaga attctcagaa gtagaaggaa     1440

gaattcagga ccttgagaaa tatgttgagg acactaaaat agatctctgg tcatacaacg     1500

cggagcttct tgttgccctg gagaaccaac atacaattga tctaactgac tcagaaatga     1560

acaaactgtt tgaaaaaaca aagaagcaac tgagggaaaa tgctgaggat atgggcaatg     1620

gttgtttcaa aatataccac aaatgtgaca atgcctgcat aggatcaata agaaatggaa     1680

cttatgacca caatgtgtac agggatgaag cattaaacaa ccggttccag atcaagggag     1740

ttgagctgaa gtcagggtac aaagattgga tcctatggat ttcctttgcc atatcatgtt     1800

ttttgctttg tgttgctttg ttggggttca tcatgtgggc ctgccaaaag ggcaacatta     1860

ggtgcaacat ttgcatttga ctcgagtgag ctaacagggc tagactgtat gaggccgtgc     1920

ttctgggttg aattaatcag gggacgacct aaagaaaaaa caatctggac tagtgcgagc     1980

agcatttctt tttgtggcgt gaatagtgat actgtagatt ggtcttggcc agacggtgct     2040

gagttgccat tcagcattga caagtagtct gttcaaaaaa ctccttgttt ctact          2095


<210>  26
<211>  1671
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 swap virus - HA - HK14 NA ORF - HA

<400>  26
agcaaaagca ggggaaaata aaaacaacca aattgaaggc aaacctactg gtcctgttaa       60

gtgcacttgc agctgcagtt gcagacacaa tttgtatagg ctagcatgaa tccaaatcaa      120

aagataataa cgattggctc tgtttctctc accatttcca caatatgctt cttcatgcaa      180

attgccattt tgataactac tgtaacattg catttcaagc aatatgaatt caactccccc      240

ccaaacaacc aagtgatgct gtgtgaacca acaataatag aaagaaacat aacagaaata      300

gtgtatttaa ctaacaccac catagagaag gaaatatgcc ccaaaccagc agaatacaga      360

aattggtcaa aaccgcaatg tggcattaca ggatttgcac ctttctctaa ggacaattcg      420

attaggcttt ccgctggtgg ggacatctgg gtgacaagag aaccttatgt gtcatgcgat      480

cctgacaagt gttatcaatt tgcccttgga cagggaacaa cactaaacaa cgtgcattca      540

aataacacag tacgtgatag gaccccttat cggactctat tgatgaatga gttgggtgtt      600

cctttccatc tggggaccaa gcaagtgtgc atagcatggt ccagctcaag ttgtcacgat      660

ggaaaagcat ggctgcatgt ttgtataacg ggggatgata aaaatgcaac tgctagcttc      720

atttacaatg ggaggcttgt agatagtgtt gtttcatggt ccaaagatat tctcaggacc      780

caggagtcag aatgcatttg tatcaatgga acttgtacag tagtaatgac tgatggaagt      840

gcttcaggaa aagctgatac taaaatacta ttcattgagg aggggaaaat cgttcatact      900

agcacattgt caggaagtgc tcagcatgtc gaagagtgct cttgctatcc tcgatatcct      960

ggtgtcagat gtgtctgcag agacaactgg aagggctcca atcggcccat cgtagatata     1020

aacataaagg atcatagcat tgtttccagt tatgtgtgtt caggacttgt tggagacaca     1080

cccagaaaaa acgacagctc cagcagtagc cattgtttgg atcctaacaa tgaagaaggt     1140

ggtcatggag tgaaaggctg ggcctttgat gatggaaatg acgtgtggat gggaagaaca     1200

atcaacgaga cgtcacgctt agggtatgaa accttcaaag tcattgaagg ctggtccaac     1260

cctaagtcca aattgcagac aaataggcaa gtcatagttg acagaggtga taggtccggt     1320

tattctggta ttttctctgt tgaaggcaaa agctgcatca atcggtgctt ttatgtggag     1380

ttgattaggg gaagaaaaga ggaaactgaa gtcttgtgga cctcaaacag tattgttgtg     1440

ttttgtggca cctcaggtac atatggaaca ggctcatggc ctgatggggc ggacctcaat     1500

ctcatgccta tataactcga gatctactca actgtcgcca gttcactggt gcttttggtc     1560

tccctggggg caatcagttt ctggatgtgt tctaatggat ctttgcagtg cagaatatgc     1620

atctgagatt agaatttcag aaatatgagg aaaaacaccc ttgtttctac t              1671


<210>  27
<211>  1716
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 NA long stalk ORF between PR8 HA packaging signals

<400>  27
agcaaaagca ggggaaaata aaaacaacca aattgaaggc aaacctactg gtcctgttaa       60

gtgcacttgc agctgcagtt gcagacacaa tttgtatagg ctagcatgaa cccgaaccaa      120

aagatcacga ctatcgggag catttgctta gtggttgggt tgatcagcct aatattgcaa      180

atagggaata taatctcaat atggattagc cattcaattc aaactggaag tcaaaaccat      240

actggaatat gcaaccaaaa catcattacc tataaaaata gcacctgggt aaatcagaca      300

tatgttaaca tcagcaacac caactttgct gctggaaaca caacagagat agtgtatctg      360

accaacacca ccatagagaa gaaggacaca acttcagtga tattaaccgg caattcatct      420

ctttgtccca tccgtgggtg ggctatatac agcaaagaca atagcataag aattggttcc      480

aaaggagacg tttttgtcat aagagagccc tttatttcat gttctcactt ggaatgcagg      540

accttttttc tgacccaagg tgccttactg aatgacaagc attcaaatgg gactgttaag      600

gacagaagcc cttatagggc cttaatgagc tgccctgtcg gtgaagctcc gtccccgtac      660

aattcaagat ttgaatcggt tgcttggtca gcaagtgcat gtcatgatgg catgggctgg      720

ctaacaatcg gaatttcagg tccagataat ggagcagtgg ctgtattaaa atacaacggc      780

ataataactg aaaccataaa aagttggagg aagaaaatat tgaggacaca agagtctgaa      840

tgtgcctgtg taaatggttc atgttttact ataatgactg atggcccgag tgatgggctg      900

gcctcgtaca aaattttcaa gatcgaaaag gggaaggtta ctaaatcaat agagttgaat      960

gcacctaatt ctcactatga ggaatgttcc tgttaccctg ataccggcaa agtgatgtgt     1020

gtgtgcagag acaactggca tggttcgaac cggccatggg tgtctttcga tcaaaacctg     1080

gattatcaaa taggatacat ctgcagtggg gttttcggtg acaacccgcg tcccgaagat     1140

ggaacaggca gctgtggtcc agtgtatgtt gatggagcaa acggagtaaa gggattttca     1200

tataggtatg gtaatggtgt ttggatagga aggaccaaaa gtcacagttc cagacatggg     1260

tttgagatga tttgggatcc taatggatgg acagagactg atagtaagtt ctctgttagg     1320

caagatgttg tggcaatgac tgattggtca gggtatagcg gaagtttcgt tcaacatcct     1380

gagctaacag ggctagactg tatgaggccg tgcttctggg ttgaattaat caggggacga     1440

cctaaagaaa aaacaatctg gactagtgcg agcagcattt ctttttgtgg cgtgaatagt     1500

gacaccgtag actggagctg gccggatggc gccgaactac cgttttctat cgataaatag     1560

ctcgagatct actcaactgt cgccagttca ctggtgcttt tggtctccct gggggcaatc     1620

agtttctgga tgtgttctaa tggatctttg cagtgcagaa tatgcatctg agattagaat     1680

ttcagaaata tgaggaaaaa cacccttgtt tctact                               1716


<210>  28
<211>  1716
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HK14 N2 long stalk ORF between PR8 HA packaging signals

<400>  28
agcaaaagca ggggaaaata aaaacaacca aattgaaggc aaacctactg gtcctgttaa       60

gtgcacttgc agctgcagtt gcagacacaa tttgtatagg ctagcatgaa tccaaatcaa      120

aagataataa cgattggctc tgtttctctc accatttcca caatatgctt cttcatgcaa      180

attgccattt tgataactac tgtaacattg catttcaagc aatatgaatt caactccccc      240

ccaaacaacc aagtgatgct gtgtgaacca acaataatag aaagaaacat aacagaaata      300

gtgtatttaa ctaatcagac atatgttaac atcagcaaca ccaactttgc tgctggaaac      360

accaccatag agaaggaaat atgccccaaa ccagcagaat acagaaattg gtcaaaaccg      420

caatgtggca ttacaggatt tgcacctttc tctaaggaca attcgattag gctttccgct      480

ggtggggaca tctgggtgac aagagaacct tatgtgtcat gcgatcctga caagtgttat      540

caatttgccc ttggacaggg aacaacacta aacaacgtgc attcaaataa cacagtacgt      600

gataggaccc cttatcggac tctattgatg aatgagttgg gtgttccttt ccatctgggg      660

accaagcaag tgtgcatagc atggtccagc tcaagttgtc acgatggaaa agcatggctg      720

catgtttgta taacggggga tgataaaaat gcaactgcta gcttcattta caatgggagg      780

cttgtagata gtgttgtttc atggtccaaa gatattctca ggacccagga gtcagaatgc      840

atttgtatca atggaacttg tacagtagta atgactgatg gaagtgcttc aggaaaagct      900

gatactaaaa tactattcat tgaggagggg aaaatcgttc atactagcac attgtcagga      960

agtgctcagc atgtcgaaga gtgctcttgc tatcctcgat atcctggtgt cagatgtgtc     1020

tgcagagaca actggaaggg ctccaatcgg cccatcgtag atataaacat aaaggatcat     1080

agcattgttt ccagttatgt gtgttcagga cttgttggag acacacccag aaaaaacgac     1140

agctccagca gtagccattg tttggatcct aacaatgaag aaggtggtca tggagtgaaa     1200

ggctgggcct ttgatgatgg aaatgacgtg tggatgggaa gaacaatcaa cgagacgtca     1260

cgcttagggt atgaaacctt caaagtcatt gaaggctggt ccaaccctaa gtccaaattg     1320

cagacaaata ggcaagtcat agttgacaga ggtgataggt ccggttattc tggtattttc     1380

tctgttgaag gcaaaagctg catcaatcgg tgcttttatg tggagttgat taggggaaga     1440

aaagaggaaa ctgaagtctt gtggacctca aacagtattg ttgtgttttg tggcacctca     1500

ggtacatatg gaacaggctc atggcctgat ggggcggacc tcaatctcat gcctatataa     1560

ctcgagatct actcaactgt cgccagttca ctggtgcttt tggtctccct gggggcaatc     1620

agtttctgga tgtgttctaa tggatctttg cagtgcagaa tatgcatctg agattagaat     1680

ttcagaaata tgaggaaaaa cacccttgtt tctact                               1716


<210>  29
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding 15 amino acid sequence extension

<400>  29
aatcagacat atgttaacat cagcaacacc aactttgctg ctgga                       45


<210>  30
<211>  1885
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bris08 HA

<400>  30
agcagaagca gagcattttc taatatccac aaaatgaagg caataattgt actactcatg       60

gtagtaacat ccaatgcaga tcgaatctgc actgggataa catcgtcaaa ctcaccacat      120

gtcgtcaaaa ctgctactca aggggaggtc aatgtgactg gtgtaatacc actgacaaca      180

acacccacca aatctcattt tgcaaatctc aaaggaacag aaaccagggg gaaactatgc      240

ccaaaatgcc tcaactgcac agatctggac gtagccttgg gcagaccaaa atgcacgggg      300

aaaataccct cggcaagagt ttcaatactc catgaagtca gacctgttac atctgggtgc      360

tttcctataa tgcacgacag aacaaaaatt agacagctgc ctaaccttct ccgaggatac      420

gaacatatca ggttatcaac ccataacgtt atcaatgcag aaaatgcacc aggaggaccc      480

tacaaaattg gaacctcagg gtcttgccct aacattacca atggaaacgg atttttcgca      540

acaatggctt gggccgtccc aaaaaacgac aaaaacaaaa cagcaacaaa tccattaaca      600

atagaagtac catacatttg tacagaagga gaagaccaaa ttaccgtttg ggggttccac      660

tctgacgacg agacccaaat ggcaaagctc tatggggact caaagcccca gaagttcacc      720

tcatctgcca acggagtgac cacacattac gtttcacaga ttggtggctt cccaaatcaa      780

acagaagacg gaggactacc acaaagtggt agaattgttg ttgattacat ggtgcaaaaa      840

tctgggaaaa caggaacaat tacctatcaa aggggtattt tattgcctca aaaggtgtgg      900

tgcgcaagtg gcaggagcaa ggtaataaaa ggatccttgc ctttaattgg agaagcagat      960

tgcctccacg aaaaatacgg tggattaaac aaaagcaagc cttactacac aggggaacat     1020

gcaaaggcca taggaaattg cccaatatgg gtgaaaacac ccttgaagct ggccaatgga     1080

accaaatata gacctcctgc aaaactatta aaggaaaggg gtttcttcgg agctattgct     1140

ggtttcttag aaggaggatg ggaaggaatg attgcaggtt ggcacggata cacatcccat     1200

ggggcacatg gagtagcggt ggcagcagac cttaagagca ctcaagaggc cataaacaag     1260

ataacaaaaa atctcaactc tttgagtgag ctggaagtaa agaatcttca aagactaagc     1320

ggtgccatgg atgaactcca caacgaaata ctagaactag atgagaaagt ggatgatctc     1380

agagctgata caataagctc acaaatagaa ctcgcagtcc tgctttccaa tgaaggaata     1440

ataaacagtg aagatgaaca tctcttggcg cttgaaagaa agctgaagaa aatgctgggc     1500

ccctctgctg tagagatagg gaatggatgc tttgaaacca aacacaagtg caaccagacc     1560

tgtctcgaca gaatagctgc tggtaccttt gatgcaggag aattttctct ccccaccttt     1620

gattcactga atattactgc tgcatcttta aatgacgatg gattggataa tcatactata     1680

ctgctttact actcaactgc tgcctccagt ttggctgtaa cactgatgat agctatcttt     1740

gttgtttata tggtctccag agacaatgtt tcttgctcca tctgtctata agggaagtta     1800

agccctgtat tttcctttat tgtagtgctt gtttacttgt tgtcattaca aagaaacgtt     1860

attgaaaaat gctcttgtta ctact                                           1885


<210>  31
<211>  1564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bris08 wt NA

<400>  31
agcagaagca gagcatcttc tcaaaaccga agcaaatagg ccaaaaatga acaatgaaca       60

atgctacctt caactataca aacgttaacc ctatttctca catcaggggg agtattatta      120

tcactatatg tgtcagcttc attatcatac ttactatatt cggatatatt gctaaaattc      180

tcaccaacag aaataactgc accaacaatg ccattggatt gtgcaaacgc atcaaatgtt      240

caggctgtga accgttctgc aacaaaaggg gtgacacttc ttctcccaga accggagtgg      300

acatacccgc gtttatcttg cccgggctca acctttcaga aagcactcct aattagccct      360

catagattcg gagaaaccaa aggaaactca gctcccttga taataaggga accttttatt      420

gcttgtggac caaatgaatg caaacacttt gctctaaccc attatgcagc ccaaccaggg      480

ggatactaca atggaacaag aggagacaga aacaagctga ggcatctaat ttcagtcaaa      540

ttgggcaaaa tcccaacagt agaaaactcc attttccaca tggcagcatg gagcgggtcc      600

gcgtgccatg atggtaagga atggacatat atcggagttg atggccctga caataatgca      660

ttgctcaaag taaaatatgg agaagcatat actgacacat accattccta tgcaaacaaa      720

atcctaagaa cacaagaaag tgcctgcaat tgcatcgggg gaaattgtta tcttatgata      780

actgatggct cagcttcagg tgttagtgaa tgcagatttc ttaagattcg agagggccga      840

ataataaaag aaatatttcc aacaggaaga gtaaaacaca ctgaggaatg cacatgcgga      900

tttgccagca ataaaaccat agaatgtgcc tgtagagata acagttacac agcaaaaaga      960

ccttttgtca aattaaacgt ggagactgat acagcagaaa taagattgat gtgcacagat     1020

acttatttgg acacccccag accaaacgat ggaagcataa caggcccttg tgaatctaat     1080

ggggacaaag ggagtggagg catcaaggga ggatttgttc atcaaagaat ggaatccaag     1140

attggaaggt ggtactctcg aacgatgtct aaaactgaaa ggatggggat gggactgtat     1200

gtcaagtatg atggagaccc atgggctgac agtgatgccc tagcttttag tggagtaatg     1260

gtttcaatga aagaacctgg ttggtactcc tttggcttcg aaataaaaga taagaaatgc     1320

gatgtcccct gtattgggat agagatggta catgatggtg gaaaagagac ttggcactca     1380

gcagcaacag ccatttactg tttaatgggc tcaggacagc tgctgtggga cactgtcaca     1440

ggtgttgaca tggctctgta atggaggaat ggttgagtct gttctaaacc ctttgttcct     1500

attttgtttg aacaattgtc cttactgaac ttaattgttt ctgaaaaatg ctcttgttac     1560

tact                                                                  1564


<210>  32
<211>  1699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bris08 long stalk NA (46aa insertion bold)

<400>  32
agcagaagca gagcatcttc tcaaaaccga agcaaatagg ccaaaaatga acaatgaaca       60

atgctacctt caactataca aacgttaacc ctatttctca catcaggggg agtattatta      120

tcactatatg tgtcagcttc attatcatac ttactatatt cggatatatt gctaaaattc      180

tcaccaacac aatatgaatt caactccccc ccaaacaacc aagtgatgct gtgtgaacca      240

acaataatag aaagaaacat aacagaaata gtgtatttaa ctaatcagac atatgttaac      300

atcagcaaca ccaactttgc tgctgaaata actgcaccaa caatgccatt ggattgtgca      360

aacgcatcaa atgttcaggc tgtgaaccgt tctgcaacaa aaggggtgac acttcttctc      420

ccagaaccgg agtggacata cccgcgttta tcttgcccgg gctcaacctt tcagaaagca      480

ctcctaatta gccctcatag attcggagaa accaaaggaa actcagctcc cttgataata      540

agggaacctt ttattgcttg tggaccaaat gaatgcaaac actttgctct aacccattat      600

gcagcccaac cagggggata ctacaatgga acaagaggag acagaaacaa gctgaggcat      660

ctaatttcag tcaaattggg caaaatccca acagtagaaa actccatttt ccacatggca      720

gcatggagcg ggtccgcgtg ccatgatggt aaggaatgga catatatcgg agttgatggc      780

cctgacaata atgcattgct caaagtaaaa tatggagaag catatactga cacataccat      840

tcctatgcaa acaaaatcct aagaacacaa gaaagtgcct gcaattgcat cgggggaaat      900

tgttatctta tgataactga tggctcagct tcaggtgtta gtgaatgcag atttcttaag      960

attcgagagg gccgaataat aaaagaaata tttccaacag gaagagtaaa acacactgag     1020

gaatgcacat gcggatttgc cagcaataaa accatagaat gtgcctgtag agataacagt     1080

tacacagcaa aaagaccttt tgtcaaatta aacgtggaga ctgatacagc agaaataaga     1140

ttgatgtgca cagatactta tttggacacc cccagaccaa acgatggaag cataacaggc     1200

ccttgtgaat ctaatgggga caaagggagt ggaggcatca agggaggatt tgttcatcaa     1260

agaatggaat ccaagattgg aaggtggtac tctcgaacga tgtctaaaac tgaaaggatg     1320

gggatgggac tgtatgtcaa gtatgatgga gacccatggg ctgacagtga tgccctagct     1380

tttagtggag taatggtttc aatgaaagaa cctggttggt actcctttgg cttcgaaata     1440

aaagataaga aatgcgatgt cccctgtatt gggatagaga tggtacatga tggtggaaaa     1500

gagacttggc actcagcagc aacagccatt tactgtttaa tgggctcagg acagctgctg     1560

tgggacactg tcacaggtgt tgacatggct ctgtaatgga ggaatggttg agtctgttct     1620

aaaccctttg ttcctatttt gtttgaacaa ttgtccttac tgaacttaat tgtttctgaa     1680

aaatgctctt gttactact                                                  1699


<210>  33
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding 46 amino acid sequence extension

<400>  33
caatatgaat tcaactcccc cccaaacaac caagtgatgc tgtgtgaacc aacaataata       60

gaaagaaaca taacagaaat agtgtattta actaatcaga catatgttaa catcagcaac      120

accaactttg ctgct                                                       135


<210>  34
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding 30 amino acid sequence extension

<400>  34
aatcagacat atgttaacat cagcaacacc aactttgctg ctggaaacac aacagagata       60

gtgtatctga ccaacaccac catagagaag                                        90


<210>  35
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Puerto Rico/8/1934 N1

<400>  35

His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile Cys Asn Gln 
1               5                   10                  15      


Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Lys Asp Thr Thr Ser 
            20                  25                  30          


Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro 
        35                  40              


<210>  36
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/California/04/2009 N1

<400>  36

His Ser Ile Gln Leu Gly Asn Gln Ser Gln Ile Glu Thr Cys Asn Gln 
1               5                   10                  15      


Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln Thr Tyr Val 
            20                  25                  30          


Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val Val Ser Val 
        35                  40                  45              


Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro 
    50                  55              


<210>  37
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR8 N1-wt

<400>  37

His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile Cys Asn Gln 
1               5                   10                  15      


Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Lys Asp Thr Thr Ser 
            20                  25                  30          


Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro 
        35                  40              


<210>  38
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins15

<400>  38

His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile Cys Asn Gln 
1               5                   10                  15      


Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Asn Gln Thr Tyr Val 
            20                  25                  30          


Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Lys Asp Thr Thr Ser Val 
        35                  40                  45              


Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro 
    50                  55              


<210>  39
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PR8 N1-Ins30

<400>  39

His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile Cys Asn Gln 
1               5                   10                  15      


Asn Ile Ile Thr Tyr Lys Asn Ser Thr Trp Val Asn Gln Thr Tyr Val 
            20                  25                  30          


Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Asn Thr Thr Glu Ile Val 
        35                  40                  45              


Tyr Leu Thr Asn Thr Thr Ile Glu Lys Lys Asp Thr Thr Ser Val Ile 
    50                  55                  60                  


Leu Thr Gly Asn Ser Ser Leu Cys Pro 
65                  70              


<210>  40
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Del25

<400>  40

Thr Leu His Phe Lys Gln Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu 
1               5                   10                  15      


Lys 
    


<210>  41
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 N2-wt

<400>  41

Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn Gln 
1               5                   10                  15      


Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu Ile 
            20                  25                  30          


Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys 
        35                  40          


<210>  42
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK14 N2-Ins15

<400>  42

Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn Gln 
1               5                   10                  15      


Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu Ile 
            20                  25                  30          


Val Tyr Leu Thr Asn Gln Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe 
        35                  40                  45              


Ala Ala Gly Asn Thr Thr Ile Glu Lys 
    50                  55          


