                         SEQUENCE LISTING

<110>  Inscripta, Inc.
 
<120>  INCREASED NUCLEIC ACID-GUIDED CELL EDITING VIA A LEXA-RAD51 
       FUSION PROTEIN

<130>  INSC045PCT

<140>  PCT/US20/40389
<141>  2020-07-01

<150>  62/871,325
<151>  2019-07-08

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isolated protein domain

<400>  1

Met Lys Ala Leu Thr Ala Arg Gln Gln Glu Val Phe Asp Leu Ile Arg 
1               5                   10                  15      


Asp His Ile Ser Gln Thr Gly Met Pro Pro Thr Arg Ala Glu Ile Ala 
            20                  25                  30          


Gln Arg Leu Gly Phe Arg Ser Pro Asn Ala Ala Glu Glu His Leu Lys 
        35                  40                  45              


Ala Leu Ala Arg Lys Gly Val Ile Glu Ile Val Ser Gly Ala Ser Arg 
    50                  55                  60                  


Gly Ile Arg Leu Leu Gln Glu Glu Glu Glu Gly Leu Pro Leu Val Gly 
65                  70                  75                  80  


Arg Val Ala Ala Gly Glu Pro Leu Leu Ala Gln Gln His Ile Glu Gly 
                85                  90                  95      


His Tyr Gln Val Asp Pro Ser Leu Phe Lys Pro Asn Ala Asp Phe Leu 
            100                 105                 110         


Leu Arg Val Ser Gly Met Ser Met Lys Asp Ile Gly Ile Met Asp Gly 
        115                 120                 125             


Asp Leu Leu Ala Val His Lys Thr Gln Asp Val Arg Asn Gly Gln Val 
    130                 135                 140                 


Val Val Ala Arg Ile Asp Asp Glu Val Thr Val Lys Arg Leu Lys Lys 
145                 150                 155                 160 


Gln Gly Asn Lys Val Glu Leu Leu Pro Glu Asn Ser Glu Phe Lys Pro 
                165                 170                 175     


Ile Val Val Asp Leu Arg Gln Gln Ser Phe Thr Ile Glu Gly Leu Ala 
            180                 185                 190         


Val Gly Val Ile Arg Asn Gly Asp Trp Leu 
        195                 200         


<210>  2
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Isolated protein domain

<400>  2

Met Ser Gln Val Gln Glu Gln His Ile Ser Glu Ser Gln Leu Gln Tyr 
1               5                   10                  15      


Gly Asn Gly Ser Leu Met Ser Thr Val Pro Ala Asp Leu Ser Gln Ser 
            20                  25                  30          


Val Val Asp Gly Asn Gly Asn Gly Ser Ser Glu Asp Ile Glu Ala Thr 
        35                  40                  45              


Asn Gly Ser Gly Asp Gly Gly Gly Leu Gln Glu Gln Ala Glu Ala Gln 
    50                  55                  60                  


Gly Glu Met Glu Asp Glu Ala Tyr Asp Glu Ala Ala Leu Gly Ser Phe 
65                  70                  75                  80  


Val Pro Ile Glu Lys Leu Gln Val Asn Gly Ile Thr Met Ala Asp Val 
                85                  90                  95      


Lys Lys Leu Arg Glu Ser Gly Leu His Thr Ala Glu Ala Val Ala Tyr 
            100                 105                 110         


Ala Pro Arg Lys Asp Leu Leu Glu Ile Lys Gly Ile Ser Glu Ala Lys 
        115                 120                 125             


Ala Asp Lys Leu Leu Asn Glu Ala Ala Arg Leu Val Pro Met Gly Phe 
    130                 135                 140                 


Val Thr Ala Ala Asp Phe His Met Arg Arg Ser Glu Leu Ile Cys Leu 
145                 150                 155                 160 


Thr Thr Gly Ser Lys Asn Leu Asp Thr Leu Leu Gly Gly Gly Val Glu 
                165                 170                 175     


Thr Gly Ser Ile Thr Glu Leu Phe Gly Glu Phe Arg Thr Gly Lys Ser 
            180                 185                 190         


Gln Leu Cys His Thr Leu Ala Val Thr Cys Gln Ile Pro Leu Asp Ile 
        195                 200                 205             


Gly Gly Gly Glu Gly Lys Cys Leu Tyr Ile Asp Thr Glu Gly Thr Phe 
    210                 215                 220                 


Arg Pro Val Arg Leu Val Ser Ile Ala Gln Arg Phe Gly Leu Asp Pro 
225                 230                 235                 240 


Asp Asp Ala Leu Asn Asn Val Ala Tyr Ala Arg Ala Tyr Asn Ala Asp 
                245                 250                 255     


His Gln Leu Arg Leu Leu Asp Ala Ala Ala Gln Met Met Ser Glu Ser 
            260                 265                 270         


Arg Phe Ser Leu Ile Val Val Asp Ser Val Met Ala Leu Tyr Arg Thr 
        275                 280                 285             


Asp Phe Ser Gly Arg Gly Glu Leu Ser Ala Arg Gln Met His Leu Ala 
    290                 295                 300                 


Lys Phe Met Arg Ala Leu Gln Arg Leu Ala Asp Gln Phe Gly Val Ala 
305                 310                 315                 320 


Val Val Val Thr Asn Gln Val Val Ala Gln Val Asp Gly Gly Met Ala 
                325                 330                 335     


Phe Asn Pro Asp Pro Lys Lys Pro Ile Gly Gly Asn Ile Met Ala His 
            340                 345                 350         


Ser Ser Thr Thr Arg Leu Gly Phe Lys Lys Gly Lys Gly Cys Gln Arg 
        355                 360                 365             


Leu Cys Lys Val Val Asp Ser Pro Cys Leu Pro Glu Ala Glu Cys Val 
    370                 375                 380                 


Phe Ala Ile Tyr Glu Asp Gly Val Gly Asp Pro Arg Glu Glu Asp Glu 
385                 390                 395                 400 


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  engineered protein binding sequence

<400>  3
ctgtatatat atacag                                                       16


<210>  4
<211>  208
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isolated protein domain

<400>  4

Met Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu 
1               5                   10                  15      


Leu Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala 
            20                  25                  30          


Gln Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn 
        35                  40                  45              


Lys Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His 
    50                  55                  60                  


His Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu 
65                  70                  75                  80  


Arg Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp 
                85                  90                  95      


Gly Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu 
            100                 105                 110         


Thr Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu 
        115                 120                 125             


Glu Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly 
    130                 135                 140                 


Cys Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu 
145                 150                 155                 160 


Thr Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu 
                165                 170                 175     


Leu Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu 
            180                 185                 190         


Leu Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser 
        195                 200                 205             


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  engineered protein binding sequence

<400>  5
tccctatcag tgatagaga                                                    19


<210>  6
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isolated protein domain

<400>  6

Met Ser Gln Val Gln Glu Gln His Ile Ser Glu Ser Gln Leu Gln Tyr 
1               5                   10                  15      


Gly Asn Gly Ser Leu Met Ser Thr Val Pro Ala Asp Leu Ser Gln Ser 
            20                  25                  30          


Val Val Asp Gly Asn Gly Asn Gly Ser Ser Glu Asp Ile Glu Ala Thr 
        35                  40                  45              


Asn Gly Ser Gly Asp Gly Gly Gly Leu Gln Glu Gln Ala Glu Ala Gln 
    50                  55                  60                  


Gly Glu Met Glu Asp Glu Ala Tyr Asp Glu Ala Ala Leu Gly Ser Phe 
65                  70                  75                  80  


Val Pro Ile Glu Lys Leu Gln Val Asn Gly Ile Thr Met Ala Asp Val 
                85                  90                  95      


Lys Lys Leu Arg Glu Ser Gly Leu His Thr Ala Glu Ala Val Ala Tyr 
            100                 105                 110         


Ala Pro Arg Lys Asp Leu Leu Glu Ile Lys Gly Ile Ser Glu Ala Lys 
        115                 120                 125             


Ala Asp Lys Leu Leu Asn Glu Ala Ala Arg Leu Val Pro Met Gly Phe 
    130                 135                 140                 


Val Thr Ala Ala Asp Phe His Met Arg Arg Ser Glu Leu Ile Cys Leu 
145                 150                 155                 160 


Thr Thr Gly Ser Lys Asn Leu Asp Thr Leu Leu Gly Gly Gly Val Glu 
                165                 170                 175     


Thr Gly Ser Ile Thr Glu Leu Phe Gly Glu Phe Arg Thr Gly Lys Ser 
            180                 185                 190         


Gln Leu Cys His Thr Leu Ala Val Thr Cys Gln Ile Pro Leu Asp Ile 
        195                 200                 205             


Gly Gly Gly Glu Gly Lys Cys Leu Tyr Ile Asp Thr Glu Gly Thr Phe 
    210                 215                 220                 


Arg Pro Val Arg Leu Val Ser Ile Ala Gln Arg Phe Gly Leu Asp Pro 
225                 230                 235                 240 


Asp Asp Ala Leu Asn Asn Val Ala Tyr Ala Arg Ala Tyr Asn Ala Asp 
                245                 250                 255     


His Gln Leu Arg Leu Leu Asp Ala Ala Ala Gln Met Met Ser Glu Ser 
            260                 265                 270         


Arg Phe Ser Leu Ile Val Val Asp Ser Val Met Ala Leu Tyr Arg Thr 
        275                 280                 285             


Asp Phe Ser Gly Arg Gly Glu Leu Ser Ala Arg Gln Met His Leu Ala 
    290                 295                 300                 


Lys Phe Met Arg Ala Leu Gln Arg Leu Ala Asp Gln Phe Gly Val Ala 
305                 310                 315                 320 


Val Val Val Thr Asn Gln Val Val Ala Gln Val Asp Gly Gly Met Ala 
                325                 330                 335     


Phe Asn Pro Asp Pro Lys Lys Pro Ile Gly Gly Asn Ile Met Ala His 
            340                 345                 350         


Ser Ser Thr Thr Arg Leu Gly Phe Lys Lys Gly Lys Gly Cys Gln Arg 
        355                 360                 365             


Leu Cys Lys Val Val Asp Ser Pro Cys Leu Pro Glu Ala Glu Cys Val 
    370                 375                 380                 


Phe Ala Ile Tyr Glu Asp Gly Val Gly Asp Pro Arg Glu Glu Asp Glu 
385                 390                 395                 400 


<210>  7
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  engineered protein binding sequence


<220>
<221>  N
<222>  (4)..(14)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (4)..(14)

<220>
<221>  misc_feature
<222>  (4)..(14)
<223>  n is a, c, g, or t

<400>  7
cggnnnnnnn nnnnccg                                                      17


<210>  8
<211>  359
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isolated protein binding domain

<400>  8

Met Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      


Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          


Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              


Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  


Gly Val Ala Thr Ser Ser Ala Leu His Ala Pro Ser Gln Ile Val Ala 
65                  70                  75                  80  


Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val Ser 
                85                  90                  95      


Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His Asn 
            100                 105                 110         


Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu Asp 
        115                 120                 125             


Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro Ala 
    130                 135                 140                 


Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile Phe 
145                 150                 155                 160 


Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala Leu 
                165                 170                 175     


Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val Ser 
            180                 185                 190         


Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn Gln 
        195                 200                 205             


Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser Gly 
    210                 215                 220                 


Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr Ala 
225                 230                 235                 240 


Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala Ile 
                245                 250                 255     


Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly Tyr 
            260                 265                 270         


Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Leu Thr Thr Ile 
        275                 280                 285             


Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu Leu 
    290                 295                 300                 


Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro Val 
305                 310                 315                 320 


Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr Ala 
                325                 330                 335     


Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln Val 
            340                 345                 350         


Ser Arg Leu Glu Ser Gly Gln 
        355                 


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  engineered protein binding sequence

<400>  9
aattgtgagc ggataacaat t                                                 21


