                         SEQUENCE LISTING

<110>  New York Blood Center, Inc.
 
<120>  Inhibitors of Zika Virus Infection

<130>  1958427.00315

<140>  PCT/US2020/047233
<141>  2020-08-20

<150>  US62/889,246
<151>  2019-08-20

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  505
<212>  PRT
<213>  Zika Virus

<400>  1

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala Asp 
            500                 505 


<210>  2
<211>  112
<212>  PRT
<213>  Zika Virus

<400>  2

Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
1               5                   10                  15      


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            20                  25                  30          


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        35                  40                  45              


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    50                  55                  60                  


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
65                  70                  75                  80  


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                85                  90                  95      


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            100                 105                 110         


<210>  3
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zika virus NS2B+NS3 fusion protein

<400>  3

Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val Gly Leu Ile Cys Ala 
1               5                   10                  15      


Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile Glu Met Ala Gly Pro Met 
            20                  25                  30          


Ala Ala Val Gly Leu Leu Ile Val Ser Tyr Val Val Ser Gly Lys Ser 
        35                  40                  45              


Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
    50                  55                  60                  


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
65                  70                  75                  80  


Ser Gly Asp Phe Ser Leu Val Glu Glu Asp Gly Pro Pro Met Arg Glu 
                85                  90                  95      


Ile Ile Leu Lys Val Val Leu Met Ala Ile Cys Gly Met Asn Pro Ile 
            100                 105                 110         


Ala Ile Pro Phe Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly 
        115                 120                 125             


Lys Arg Ser Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys 
    130                 135                 140                 


Lys Gly Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu 
145                 150                 155                 160 


Leu Gly Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe 
                165                 170                 175     


His Thr Met Trp His Val Thr Lys Gly Ala Ala Leu Arg Ser Gly Glu 
            180                 185                 190         


Gly Arg Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu Val Ser 
        195                 200                 205             


Tyr Cys Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly Leu Ser Glu 
    210                 215                 220                 


Val Gln Leu Leu Ala Val Pro Pro Gly Glu Arg Ala Arg Asn Ile Gln 
225                 230                 235                 240 


Thr Leu Pro Gly Ile Phe Lys Thr Lys Asp Gly Asp Ile Gly Ala Val 
                245                 250                 255     


Ala Leu Asp Tyr Pro Ala Gly Thr Ser Gly Ser Pro Ile Leu Asp Lys 
            260                 265                 270         


Cys Gly Arg Val Ile Gly Leu Tyr Gly Asn Gly Val Val Ile Lys Asn 
        275                 280                 285             


Gly Ser Tyr Val Ser Ala Ile Thr Gln Gly Lys Arg Glu Glu Glu Thr 
    290                 295                 300                 


Pro Val Glu Cys Phe Glu Pro Ser Met Leu Lys Lys Lys Gln Leu Thr 
305                 310                 315                 320 


Val Leu Asp Leu His Pro Gly Ala Gly Lys Thr Arg Arg Val Leu Pro 
                325                 330                 335     


Glu Ile Val Arg Glu Ala Ile Lys Lys Arg Leu Arg Thr Val Ile Leu 
            340                 345                 350         


Ala Pro Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly 
        355                 360                 365             


Leu Pro Val Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly 
    370                 375                 380                 


Thr Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu 
385                 390                 395                 400 


Leu Gln Pro Ile Arg Val Pro Asn Tyr Asn Leu Tyr Ile Met Asp Glu 
                405                 410                 415     


Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile Ser 
            420                 425                 430         


Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr Ala Thr 
        435                 440                 445             


Pro Pro Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser Pro Ile Met 
    450                 455                 460                 


Asp Thr Glu Val Glu Val Pro Glu Arg Ala Trp Ser Ser Gly Phe Asp 
465                 470                 475                 480 


Trp Val Thr Asp His Ser Gly Lys Thr Val Trp Phe Val Pro Ser Val 
                485                 490                 495     


Arg Asn Gly Asn Glu Ile Ala Ala Cys Leu Thr Lys Ala Gly Lys Arg 
            500                 505                 510         


Val Ile Gln Leu Ser Arg Lys Thr Phe Glu Thr Glu Phe Gln Lys Thr 
        515                 520                 525             


Lys Asn Gln Glu Trp Asp Phe Val Ile Thr Thr Asp Ile Ser Glu Met 
    530                 535                 540                 


Gly Ala Asn Phe Lys Ala Asp Arg Val Ile Asp Ser Arg Arg Cys Leu 
545                 550                 555                 560 


Lys Pro Val Ile Leu Asp Gly Glu Arg Val Ile Leu Ala Gly Pro Met 
                565                 570                 575     


Pro Val Thr His Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 
            580                 585                 590         


Asn Pro Asn Lys Pro Gly Asp Glu Tyr Met Tyr Gly Gly Gly Cys Ala 
        595                 600                 605             


Glu Thr Asp Glu Gly His Ala His Trp Leu Glu Ala Arg Met Leu Leu 
    610                 615                 620                 


Asp Asn Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro 
625                 630                 635                 640 


Glu Ala Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr 
                645                 650                 655     


Glu Gln Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu Pro 
            660                 665                 670         


Val Trp Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr Thr Asp 
        675                 680                 685             


Arg Arg Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile Met Glu Asp 
    690                 695                 700                 


Ser Val Pro Ala Glu Val Trp Thr Lys Tyr Gly Glu Lys Arg Val Leu 
705                 710                 715                 720 


Lys Pro Arg Trp Met Asp Ala Arg Val Cys Ser Asp His Ala Ala Leu 
                725                 730                 735     


Lys Ser Phe Lys Glu Phe Ala Ala Gly Lys Arg 
            740                 745         


<210>  4
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zika virus NS2B-NS3 fusion protein

<400>  4

Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
1               5                   10                  15      


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
            20                  25                  30          


Ser Gly Asp Phe Ser Leu Val Glu Glu Asp Gly Pro Pro Met Arg Glu 
        35                  40                  45              


Ile Ser Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys 
    50                  55                  60                  


Gly Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu 
65                  70                  75                  80  


Gly Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe His 
                85                  90                  95      


Thr Met Trp His Val Thr Lys Gly Ala Ala Leu Arg Ser Gly Glu Gly 
            100                 105                 110         


Arg Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu Val Ser Tyr 
        115                 120                 125             


Cys Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly Leu Ser Glu Val 
    130                 135                 140                 


Gln Leu Leu Ala Val Pro Pro Gly Glu Arg Ala Arg Asn Ile Gln Thr 
145                 150                 155                 160 


Leu Pro Gly Ile Phe Lys Thr Lys Asp Gly Asp Ile Gly Ala Val Ala 
                165                 170                 175     


Leu Asp Tyr Pro Ala Gly Thr Ser Gly Ser Pro Ile Leu Asp Lys Cys 
            180                 185                 190         


Gly Arg Val Ile Gly Leu Tyr Gly Asn Gly Val Val Ile Lys Asn Gly 
        195                 200                 205             


Ser Tyr Val Ser Ala Ile Thr Gln Gly Lys Arg Glu Glu Glu Thr Pro 
    210                 215                 220                 


Val Glu Cys Phe Glu Pro Ser Met Leu Lys 
225                 230                 


<210>  5
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zika virus NS2B-NS3 fusion protein

<400>  5

Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
1               5                   10                  15      


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
            20                  25                  30          


Ser Gly Asp Phe Ser Leu Val Glu Glu Asp Gly Pro Pro Met Arg Glu 
        35                  40                  45              


Ile Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser Gly Ala Leu Trp Asp 
    50                  55                  60                  


Val Pro Ala Pro Lys Glu Val Lys Lys Gly Glu Thr Thr Asp Gly Val 
65                  70                  75                  80  


Tyr Arg Val Met Thr Arg Arg Leu Leu Gly Ser Thr Gln Val Gly Val 
                85                  90                  95      


Gly Val Met Gln Glu Gly Val Phe His Thr Met Trp His Val Thr Lys 
            100                 105                 110         


Gly Ala Ala Leu Arg Ser Gly Glu Gly Arg Leu Asp Pro Tyr Trp Gly 
        115                 120                 125             


Asp Val Lys Gln Asp Leu Val Ser Tyr Cys Gly Pro Trp Lys Leu Asp 
    130                 135                 140                 


Ala Ala Trp Asp Gly Val Ser Glu Val Gln Leu Leu Ala Val Pro Pro 
145                 150                 155                 160 


Gly Glu Arg Ala Arg Asn Ile Gln Thr Leu Pro Gly Ile Phe Lys Thr 
                165                 170                 175     


Lys Asp Gly Asp Ile Gly Ala Val Ala Leu Asp Tyr Pro Ala Gly Thr 
            180                 185                 190         


Ser Gly Ser Pro Ile Leu Asp Lys Cys Gly Arg Val Ile Gly Leu Tyr 
        195                 200                 205             


Gly Asn Gly Val Val Ile Lys Asn Gly Ser Tyr Val Ser Ala Ile Thr 
    210                 215                 220                 


Gln Gly Lys Arg Glu Glu Glu Thr Pro Val Glu Cys Phe Glu Pro Ser 
225                 230                 235                 240 


Met Leu Lys 
            


