                         SEQUENCE LISTING

<110>  University of Pittsburgh - Of the Commonwealth System of 
       Higher Education
       The Regents of the University of California
 
<120>  ADENO-ASSOCIATED VIRUSES AND METHODS AND MATERIALS FOR MAKING AND
        USING ADENO-ASSOCIATED VIRUSES

<130>  45049-0042WO1

<140>  PCT/US2020/048592
<141>  2020-08-28

<150>  US 62/892,959
<151>  2019-08-28

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  1

Asp Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
1               5                   10              


<210>  2
<211>  735
<212>  PRT
<213>  Adeno-Associated Virus Type 2

<400>  2

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr 
            580                 585                 590         


Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp 
        595                 600                 605             


Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr 
    610                 615                 620                 


Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys 
625                 630                 635                 640 


His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn 
                645                 650                 655     


Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln 
            660                 665                 670         


Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys 
        675                 680                 685             


Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr 
    690                 695                 700                 


Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr 
705                 710                 715                 720 


Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735 


<210>  3
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  3

Ala Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
1               5                   10              


<210>  4
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  4

Asp His Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
1               5                   10              


<210>  5
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  5

Asp Pro Ile Val Met Val Asp Asn Asp Lys Pro Ile Thr 
1               5                   10              


<210>  6
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  6

Asp Pro Ile Val Met Ile Asp Ala Asp Lys Pro Ile Thr 
1               5                   10              


<210>  7
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  7

Asp Pro Ile Val Met Ile Asp Asn Tyr Lys Pro Ile Thr 
1               5                   10              


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  8

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  9

Lys Leu Gly Asp Ile Glu Phe Ile Lys Val Asn Lys 
1               5                   10          


<210>  10
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (44)..(44)
<223>  Xaa = D or V

<400>  10

Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg Asp Ile Asn Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn Leu Ser Gly Gln Thr 
            20                  25                  30          


Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys Xaa Phe Tyr Leu Met 
        35                  40                  45              


Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala Pro Glu Gly Tyr Glu 
    50                  55                  60                  


Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu Lys Gly Gln Ile Trp 
65                  70                  75                  80  


Val Asp Ser 
            


<210>  11
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa can be any naturally occurring amino acid

<400>  11

Asp Ser Thr Thr His Xaa Lys Phe Ser Lys Arg Asp Ile Asn Gly Xaa 
1               5                   10                  15      


Glu Leu Ala Gly Ala Xaa Ile Glu Leu Arg Asn Xaa Ser Gly Xaa Thr 
            20                  25                  30          


Ile Gln Ser Trp Xaa Ser Asp Gly Xaa Val Lys Asp Phe Tyr Leu Met 
        35                  40                  45              


Pro Gly Xaa Tyr Gln Phe Val Glu Thr Ala Ala Pro Glu Gly Tyr Glu 
    50                  55                  60                  


Leu Xaa Ala Pro Ile Thr Phe Thr Ile Asp Glu Xaa Gly Gln Ile Trp 
65                  70                  75                  80  


Val Xaa Ser 
            


<210>  12
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (65)..(65)
<223>  Xaa = D or V

<400>  12

Ile Asp Thr Met Ser Gly Leu Ser Gly Glu Thr Gly Gln Ser Gly Asn 
1               5                   10                  15      


Thr Thr Ile Glu Glu Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg 
            20                  25                  30          


Asp Ile Asn Gly Lys Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn 
        35                  40                  45              


Leu Ser Gly Gln Thr Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys 
    50                  55                  60                  


Xaa Phe Tyr Leu Met Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala 
65                  70                  75                  80  


Pro Glu Gly Tyr Glu Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu 
                85                  90                  95      


Lys Gly Gln Ile Trp Val Asp Ser Thr Leu Ile Val Gly Asp Asp Pro 
            100                 105                 110         


Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
        115                 120             


<210>  13
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (52)..(52)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MOD_RES
<222>  (65)..(65)
<223>  Xaa = D or V

<220>
<221>  misc_feature
<222>  (72)..(72)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (103)..(103)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (105)..(105)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  Xaa can be any naturally occurring amino acid

<400>  13

Ile Asp Thr Xaa Ser Gly Leu Ser Xaa Xaa Thr Xaa Xaa Ser Xaa Asn 
1               5                   10                  15      


Thr Thr Xaa Glu Glu Asp Ser Thr Thr His Xaa Lys Phe Ser Lys Arg 
            20                  25                  30          


Asp Ile Asn Gly Xaa Glu Leu Ala Gly Ala Xaa Ile Glu Leu Arg Asn 
        35                  40                  45              


Xaa Ser Gly Xaa Thr Ile Gln Ser Trp Xaa Ser Asp Gly Xaa Val Lys 
    50                  55                  60                  


Xaa Phe Tyr Leu Met Pro Gly Xaa Tyr Gln Phe Val Glu Thr Ala Ala 
65                  70                  75                  80  


Pro Glu Gly Tyr Glu Leu Xaa Ala Pro Ile Thr Phe Thr Ile Asp Glu 
                85                  90                  95      


Xaa Gly Gln Ile Trp Val Xaa Ser Xaa Leu Ile Val Gly Asp Asp Pro 
            100                 105                 110         


Xaa Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
        115                 120             


<210>  14
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (64)..(64)
<223>  Xaa = D or V

<400>  14

Asp Thr Met Ser Gly Leu Ser Gly Glu Thr Gly Gln Ser Gly Asn Thr 
1               5                   10                  15      


Thr Ile Glu Glu Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg Asp 
            20                  25                  30          


Ile Asn Gly Lys Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn Leu 
        35                  40                  45              


Ser Gly Gln Thr Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys Xaa 
    50                  55                  60                  


Phe Tyr Leu Met Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala Pro 
65                  70                  75                  80  


Glu Gly Tyr Glu Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu Lys 
                85                  90                  95      


Gly Gln Ile Trp Val Asp Ser Thr Leu Ile Val Gly Asp Asp Pro 
            100                 105                 110     


<210>  15
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (64)..(64)
<223>  Xaa = D or V

<400>  15

Asp Thr Met Ser Gly Leu Ser Gly Glu Thr Gly Gln Ser Gly Asn Thr 
1               5                   10                  15      


Thr Ile Glu Glu Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg Asp 
            20                  25                  30          


Ile Asn Gly Lys Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn Leu 
        35                  40                  45              


Ser Gly Gln Thr Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys Xaa 
    50                  55                  60                  


Phe Tyr Leu Met Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala Pro 
65                  70                  75                  80  


Glu Gly Tyr Glu Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu Lys 
                85                  90                  95      


Gly Gln Ile Trp Val Asp Ser 
            100             


<210>  16
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (44)..(44)
<223>  Xaa = D or V

<400>  16

Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg Asp Ile Asn Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn Leu Ser Gly Gln Thr 
            20                  25                  30          


Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys Xaa Phe Tyr Leu Met 
        35                  40                  45              


Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala Pro Glu Gly Tyr Glu 
    50                  55                  60                  


Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu Lys Gly Gln Ile Trp 
65                  70                  75                  80  


Val Asp Ser Thr Leu Ile Val Gly Asp Asp Pro Ile Val Met Ile Asp 
                85                  90                  95      


Asn Asp Lys Pro Ile Thr 
            100         


<210>  17
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (64)..(64)
<223>  Xaa = D or V

<400>  17

Asp Thr Met Ser Gly Leu Ser Gly Glu Thr Gly Gln Ser Gly Asn Thr 
1               5                   10                  15      


Thr Ile Glu Glu Asp Ser Thr Thr His Val Lys Phe Ser Lys Arg Asp 
            20                  25                  30          


Ile Asn Gly Lys Glu Leu Ala Gly Ala Met Ile Glu Leu Arg Asn Leu 
        35                  40                  45              


Ser Gly Gln Thr Ile Gln Ser Trp Val Ser Asp Gly Thr Val Lys Xaa 
    50                  55                  60                  


Phe Tyr Leu Met Pro Gly Thr Tyr Gln Phe Val Glu Thr Ala Ala Pro 
65                  70                  75                  80  


Glu Gly Tyr Glu Leu Ala Ala Pro Ile Thr Phe Thr Ile Asp Glu Lys 
                85                  90                  95      


Gly Gln Ile Trp Val Asp Ser Thr Leu Ile Val Gly Asp Asp Pro Ile 
            100                 105                 110         


Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
        115                 120         


<210>  18
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety for peptide tag

<400>  18

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  


Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
                85                  90          


<210>  19
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety for peptide tag

<400>  19

Gly Ser His Met Lys Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys 
1               5                   10                  15      


Gln His Pro Asp Tyr Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly 
            20                  25                  30          


Thr Tyr Gln Asn Val Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Lys 
        35                  40                  45              


Asn Leu Ser Asp Gly Lys Tyr Arg Leu Phe Glu Asn Ser Glu Pro Ala 
    50                  55                  60                  


Gly Tyr Lys Pro Val Gln Asn Lys Pro Ile Val Ala Phe Gln Ile Val 
65                  70                  75                  80  


Asn Gly Glu Val Arg Asp Val Thr Ser Ile Val Pro Gln Asp Ile Pro 
                85                  90                  95      


Ala Thr Tyr Glu Phe Thr Asn Gly Lys His Tyr Ile Thr Asn Glu Pro 
            100                 105                 110         


Ile Pro Pro Lys 
        115     


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site

<400>  20

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site


<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Xaa = Q or G

<400>  21

Leu Glu Val Leu Phe Xaa Pro 
1               5           


<210>  22
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site

<400>  22

Leu Val Pro Arg Gly Ser 
1               5       


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa = E or P

<400>  23

Ile Xaa Gly Arg 
1               


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site

<400>  24

Asp Asp Asp Asp Lys 
1               5   


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protease cleavage site

<400>  25

Leu Val Pro Trp Glu Leu Gln Glu 
1               5               


<210>  26
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Xaa = valine, alanine, isoleucine, or leucine

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Xaa = lysine, arginine, histidine, or serine

<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  Xaa = methionine, leucine, phenylalanine, or tryptophan

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Xaa = leucine, methionine, isoleucine, or tyrosine

<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa = glutamine, serine, threonine, or asparagine

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  Xaa = isoleucine, leucine, methionine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (41)..(41)
<223>  Xaa = threonine, serine, valine, or asparagine

<220>
<221>  MOD_RES
<222>  (44)..(44)
<223>  Xaa = D or V

<220>
<221>  MOD_RES
<222>  (51)..(51)
<223>  Xaa = threonine, serine, valine, or asparagine

<220>
<221>  MOD_RES
<222>  (66)..(66)
<223>  Xaa = alanine, isoleucine, leucine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (76)..(76)
<223>  Xaa = lysine, arginine, histidine, or serine

<220>
<221>  MOD_RES
<222>  (82)..(82)
<223>  Xaa = aspartic acid, glutamic acid, or lysine

<400>  26

Asp Ser Thr Thr His Xaa Lys Phe Ser Lys Arg Asp Ile Asn Gly Xaa 
1               5                   10                  15      


Glu Leu Ala Gly Ala Xaa Ile Glu Leu Arg Asn Xaa Ser Gly Xaa Thr 
            20                  25                  30          


Ile Gln Ser Trp Xaa Ser Asp Gly Xaa Val Lys Xaa Phe Tyr Leu Met 
        35                  40                  45              


Pro Gly Xaa Tyr Gln Phe Val Glu Thr Ala Ala Pro Glu Gly Tyr Glu 
    50                  55                  60                  


Leu Xaa Ala Pro Ile Thr Phe Thr Ile Asp Glu Xaa Gly Gln Ile Trp 
65                  70                  75                  80  


Val Xaa Ser 
            


<210>  27
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety that can form a covalent bond with a capsid 
       polypeptide


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa = methionine, isoleucine, leucine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Xaa = glycine, proline, cysteine, or serine

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Xaa = glutamic acid, aspartic acid, arginine, or lysine

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Xaa = glycine, proline, cysteine, or serine

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Xaa = glutamine, serine, threonine, or asparagine

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Xaa = glycine, proline, cysteine, or serine

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  Xaa = isoleucine, leucine, methionine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (27)..(27)
<223>  Xaa = valine, alanine, isoleucine, or leucine

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  Xaa = lysine, arginine, histidine, or serine

<220>
<221>  MOD_RES
<222>  (43)..(43)
<223>  Xaa = methionine, leucine, phenylalanine, or tryptophan

<220>
<221>  MOD_RES
<222>  (49)..(49)
<223>  Xaa = leucine, methionine, isoleucine, or tyrosine

<220>
<221>  MOD_RES
<222>  (52)..(52)
<223>  Xaa = glutamine, serine, threonine, or asparagine

<220>
<221>  MOD_RES
<222>  (58)..(58)
<223>  Xaa = isoleucine, leucine, methionine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (62)..(62)
<223>  Xaa = threonine, serine, valine, or asparagine

<220>
<221>  MOD_RES
<222>  (65)..(65)
<223>  Xaa = D or V

<220>
<221>  MOD_RES
<222>  (72)..(72)
<223>  Xaa = threonine, serine, valine, or asparagine

<220>
<221>  MOD_RES
<222>  (87)..(87)
<223>  Xaa = alanine, isoleucine, leucine, or phenylalanine

<220>
<221>  MOD_RES
<222>  (97)..(97)
<223>  Xaa = lysine, arginine, histidine, or serine

<220>
<221>  MOD_RES
<222>  (103)..(103)
<223>  Xaa = aspartic acid, glutamic acid, or lysine

<220>
<221>  MOD_RES
<222>  (105)..(105)
<223>  Xaa = threonine, serine, valine, or asparagine

<220>
<221>  MOD_RES
<222>  (113)..(113)
<223>  Xaa = isoleucine, leucine, methionine, or phenylalanine

<400>  27

Ile Asp Thr Xaa Ser Gly Leu Ser Xaa Xaa Thr Xaa Xaa Ser Xaa Asn 
1               5                   10                  15      


Thr Thr Xaa Glu Glu Asp Ser Thr Thr His Xaa Lys Phe Ser Lys Arg 
            20                  25                  30          


Asp Ile Asn Gly Xaa Glu Leu Ala Gly Ala Xaa Ile Glu Leu Arg Asn 
        35                  40                  45              


Xaa Ser Gly Xaa Thr Ile Gln Ser Trp Xaa Ser Asp Gly Xaa Val Lys 
    50                  55                  60                  


Xaa Phe Tyr Leu Met Pro Gly Xaa Tyr Gln Phe Val Glu Thr Ala Ala 
65                  70                  75                  80  


Pro Glu Gly Tyr Glu Leu Xaa Ala Pro Ile Thr Phe Thr Ile Asp Glu 
                85                  90                  95      


Xaa Gly Gln Ile Trp Val Xaa Ser Xaa Leu Ile Val Gly Asp Asp Pro 
            100                 105                 110         


Xaa Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
        115                 120             


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide primer

<400>  28
agacaagcag ctaccgcaga t                                                 21


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide primer

<400>  29
gttgcctctc tggaggttgg                                                   20


<210>  30
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide amplicon sequence

<400>  30
ctagcagacc ctattgtaat gattgataat gacaagccga ttactgct                    48


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide primer

<400>  31
agacaagcag ctaccgcaga t                                                 21


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide primer

<400>  32
gttgcctctc tggaggttgg                                                   20


<210>  33
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide amplicon sequence

<400>  33
accggtgacc ctattgtaat gattgataat gacaagccga ttactggctt aagt             54


<210>  34
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide amplicon sequence

<400>  34
ctagcagaga acctctattt ccagggagcc cacatcgtga tggtggacgc ctacaagccg       60

acgaaggct                                                               69


<210>  35
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide amplicon sequence

<400>  35
accggtgaga acctctattt ccagggagcc cacatcgtga tggtggacgc ctacaagccg       60

acgaagggct taagt                                                        75


<210>  36
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  36

Asp Pro Ile Val Met Ile Asp Asn Asp Lys Pro Tyr Thr 
1               5                   10              


<210>  37
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  37

Asp Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
1               5                   10              


<210>  38
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  38

Gly Ser Asp Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr Gly 
1               5                   10                  15      


Ser 
    


<210>  39
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  39

Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile Thr 
1               5                   10          


<210>  40
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  capsid polypeptide

<400>  40

Pro Ile Val Met Ile Asp Asn Asp Lys Pro Ile 
1               5                   10      


<210>  41
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  41

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  42

Leu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ala 
1               5                   10                  15  


<210>  43
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  43

Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Pro Ala Asn Leu Lys Ala 
1               5                   10                  15      


Leu Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln Ala Ala Glu Glu Leu 
            20                  25                  30          


Ala Asn Ala Lys Lys Leu Lys Glu Gln Leu Glu Lys Gly Ser Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser 
    50                  


<210>  44
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  44

Val Pro Thr Ile Val Met Val Asp Ala Tyr Lys Arg Tyr Lys 
1               5                   10                  


<210>  45
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  binding moiety for peptide tag

<400>  45

Ala Met Val Thr Thr Leu Ser Gly Leu Ser Gly Glu Gln Gly Pro Ser 
1               5                   10                  15      


Gly Asp Met Thr Thr Glu Glu Asp Ser Ala Thr His Ile Lys Phe Ser 
            20                  25                  30          


Lys Arg Asp Glu Asp Gly Arg Glu Leu Ala Gly Ala Thr Met Glu Leu 
        35                  40                  45              


Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp Gly His 
    50                  55                  60                  


Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr 
65                  70                  75                  80  


Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala Ile Thr Phe Thr Val 
                85                  90                  95      


Asn Glu Gln Gly Gln Val Thr Val Asn Gly Glu Ala Thr Lys Gly Asp 
            100                 105                 110         


Ala His Thr Gly Ser Ser Gly Ser 
        115                 120 


<210>  46
<211>  751
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of AAV2-588-LA-AAS-A

<400>  46

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Leu Ala Asp Pro Ile 
            580                 585                 590         


Val Met Ile Asp Asn Asp Lys Pro Ile Thr Ala Arg Gln Ala Ala Thr 
        595                 600                 605             


Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp 
    610                 615                 620                 


Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr 
625                 630                 635                 640 


Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys 
                645                 650                 655     


His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn 
            660                 665                 670         


Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln 
        675                 680                 685             


Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys 
    690                 695                 700                 


Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr 
705                 710                 715                 720 


Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr 
                725                 730                 735     


Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
            740                 745                 750     


<210>  47
<211>  753
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of AAV2-588-TG-AAS-GLS

<400>  47

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Thr Gly Asp Pro Ile 
            580                 585                 590         


Val Met Ile Asp Asn Asp Lys Pro Ile Thr Gly Leu Ser Arg Gln Ala 
        595                 600                 605             


Ala Thr Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp 
    610                 615                 620                 


Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro 
625                 630                 635                 640 


His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly 
                645                 650                 655     


Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro 
            660                 665                 670         


Ala Asn Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile 
        675                 680                 685             


Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu 
    690                 695                 700                 


Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser 
705                 710                 715                 720 


Asn Tyr Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly 
                725                 730                 735     


Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn 
            740                 745                 750         


Leu 
    


<210>  48
<211>  758
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of AAV2-588-LA-tev-SpyTag-A

<400>  48

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Leu Ala Glu Asn Leu 
            580                 585                 590         


Tyr Phe Gln Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr 
        595                 600                 605             


Lys Ala Arg Gln Ala Ala Thr Ala Asp Val Asn Thr Gln Gly Val Leu 
    610                 615                 620                 


Pro Gly Met Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile 
625                 630                 635                 640 


Trp Ala Lys Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu 
                645                 650                 655     


Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys 
            660                 665                 670         


Asn Thr Pro Val Pro Ala Asn Pro Ser Thr Thr Phe Ser Ala Ala Lys 
        675                 680                 685             


Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu 
    690                 695                 700                 


Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu 
705                 710                 715                 720 


Ile Gln Tyr Thr Ser Asn Tyr Asn Lys Ser Val Asn Val Asp Phe Thr 
                725                 730                 735     


Val Asp Thr Asn Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg 
            740                 745                 750         


Tyr Leu Thr Arg Asn Leu 
        755             


<210>  49
<211>  760
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of AAV2-588-TG-tev-SpyTag-GLS

<400>  49

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Thr Gly Glu Asn Leu 
            580                 585                 590         


Tyr Phe Gln Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr 
        595                 600                 605             


Lys Gly Leu Ser Arg Gln Ala Ala Thr Ala Asp Val Asn Thr Gln Gly 
    610                 615                 620                 


Val Leu Pro Gly Met Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly 
625                 630                 635                 640 


Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly His Phe His Pro Ser 
                645                 650                 655     


Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu 
            660                 665                 670         


Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Ser Thr Thr Phe Ser Ala 
        675                 680                 685             


Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser 
    690                 695                 700                 


Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn 
705                 710                 715                 720 


Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn Lys Ser Val Asn Val Asp 
                725                 730                 735     


Phe Thr Val Asp Thr Asn Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly 
            740                 745                 750         


Thr Arg Tyr Leu Thr Arg Asn Leu 
        755                 760 


