                         SEQUENCE LISTING

<110>  Lygos, Inc.
 
<120>  SYSTEMS AND METHODS FOR PREPARING CANNABINOIDS AND DERIVATIVES

<130>  LYGOS-0035-01-WO

<150>  US62/894552
<151>  2019-08-30

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  385
<212>  PRT
<213>  Cannabis sativa

<400>  1

Met Asn His Leu Arg Ala Glu Gly Pro Ala Ser Val Leu Ala Ile Gly 
1               5                   10                  15      


Thr Ala Asn Pro Glu Asn Ile Leu Leu Gln Asp Glu Phe Pro Asp Tyr 
            20                  25                  30          


Tyr Phe Arg Val Thr Lys Ser Glu His Met Thr Gln Leu Lys Glu Lys 
        35                  40                  45              


Phe Arg Lys Ile Cys Asp Lys Ser Met Ile Arg Lys Arg Asn Cys Phe 
    50                  55                  60                  


Leu Asn Glu Glu His Leu Lys Gln Asn Pro Arg Leu Val Glu His Glu 
65                  70                  75                  80  


Met Gln Thr Leu Asp Ala Arg Gln Asp Met Leu Val Val Glu Val Pro 
                85                  90                  95      


Lys Leu Gly Lys Asp Ala Cys Ala Lys Ala Ile Lys Glu Trp Gly Gln 
            100                 105                 110         


Pro Lys Ser Lys Ile Thr His Leu Ile Phe Thr Ser Ala Ser Thr Thr 
        115                 120                 125             


Asp Met Pro Gly Ala Asp Tyr His Cys Ala Lys Leu Leu Gly Leu Ser 
    130                 135                 140                 


Pro Ser Val Lys Arg Val Met Met Tyr Gln Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 


Gly Thr Val Leu Arg Ile Ala Lys Asp Ile Ala Glu Asn Asn Lys Gly 
                165                 170                 175     


Ala Arg Val Leu Ala Val Cys Cys Asp Ile Met Ala Cys Leu Phe Arg 
            180                 185                 190         


Gly Pro Ser Glu Ser Asp Leu Glu Leu Leu Val Gly Gln Ala Ile Phe 
        195                 200                 205             


Gly Asp Gly Ala Ala Ala Val Ile Val Gly Ala Glu Pro Asp Glu Ser 
    210                 215                 220                 


Val Gly Glu Arg Pro Ile Phe Glu Leu Val Ser Thr Gly Gln Thr Ile 
225                 230                 235                 240 


Leu Pro Asn Ser Glu Gly Thr Ile Gly Gly His Ile Arg Glu Ala Gly 
                245                 250                 255     


Leu Ile Phe Asp Leu His Lys Asp Val Pro Met Leu Ile Ser Asn Asn 
            260                 265                 270         


Ile Glu Lys Cys Leu Ile Glu Ala Phe Thr Pro Ile Gly Ile Ser Asp 
        275                 280                 285             


Trp Asn Ser Ile Phe Trp Ile Thr His Pro Gly Gly Lys Ala Ile Leu 
    290                 295                 300                 


Asp Lys Val Glu Glu Lys Leu His Leu Lys Ser Asp Lys Phe Val Asp 
305                 310                 315                 320 


Ser Arg His Val Leu Ser Glu His Gly Asn Met Ser Ser Ser Thr Val 
                325                 330                 335     


Leu Phe Val Met Asp Glu Leu Arg Lys Arg Ser Leu Glu Glu Gly Lys 
            340                 345                 350         


Ser Thr Thr Gly Asp Gly Phe Glu Trp Gly Val Leu Phe Gly Phe Gly 
        355                 360                 365             


Pro Gly Leu Thr Val Glu Arg Val Val Val Arg Ser Val Pro Ile Lys 
    370                 375                 380                 


Tyr 
385 


<210>  2
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A polyketide synthase


<220>
<221>  misc_feature
<222>  (198)..(209)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Met Asn His Leu Arg Ala Glu Gly Pro Ala Ser Val Leu Ala Ile Gly 
1               5                   10                  15      


Thr Ala Asn Pro Glu Asn Ile Leu Leu Gln Asp Glu Phe Pro Asp Tyr 
            20                  25                  30          


Tyr Phe Arg Val Thr Lys Ser Glu His Met Thr Gln Leu Lys Glu Lys 
        35                  40                  45              


Phe Arg Lys Ile Cys Asp Lys Ser Met Ile Arg Lys Arg Asn Cys Phe 
    50                  55                  60                  


Leu Asn Glu Glu His Leu Lys Gln Asn Pro Arg Leu Val Glu His Glu 
65                  70                  75                  80  


Met Gln Thr Leu Asp Ala Arg Gln Asp Met Leu Val Val Glu Val Pro 
                85                  90                  95      


Lys Leu Gly Lys Asp Ala Cys Ala Lys Ala Ile Lys Glu Trp Gly Gln 
            100                 105                 110         


Pro Lys Ser Lys Ile Thr His Leu Ile Phe Thr Ser Ala Ser Thr Thr 
        115                 120                 125             


Asp Met Pro Gly Ala Asp Tyr His Cys Ala Lys Leu Leu Gly Leu Ser 
    130                 135                 140                 


Pro Ser Val Lys Arg Val Met Met Tyr Gln Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 


Gly Thr Val Leu Arg Ile Ala Lys Asp Ile Ala Glu Asn Asn Lys Gly 
                165                 170                 175     


Ala Arg Val Leu Ala Val Cys Cys Asp Ile Met Ala Cys Leu Phe Arg 
            180                 185                 190         


Gly Pro Ser Glu Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        195                 200                 205             


Xaa Asp Gly Ala Ala Ala Val Ile Val Gly Ala Glu Pro Asp Glu Ser 
    210                 215                 220                 


Val Gly Glu Arg Pro Ile Phe Glu Leu Val Ser Thr Gly Gln Thr Ile 
225                 230                 235                 240 


Leu Pro Asn Ser Glu Gly Thr Ile Gly Gly His Ile Arg Glu Ala Gly 
                245                 250                 255     


Leu Ile Phe Asp Leu His Lys Asp Val Pro Met Leu Ile Ser Asn Asn 
            260                 265                 270         


Ile Glu Lys Cys Leu Ile Glu Ala Phe Thr Pro Ile Gly Ile Ser Asp 
        275                 280                 285             


Trp Asn Ser Ile Phe Trp Ile Thr His Pro Gly Gly Lys Ala Ile Leu 
    290                 295                 300                 


Asp Lys Val Glu Glu Lys Leu His Leu Lys Ser Asp Lys Phe Val Asp 
305                 310                 315                 320 


Ser Arg His Val Leu Ser Glu His Gly Asn Met Ser Ser Ser Thr Val 
                325                 330                 335     


Leu Phe Val Met Asp Glu Leu Arg Lys Arg Ser Leu Glu Glu Gly Lys 
            340                 345                 350         


Ser Thr Thr Gly Asp Gly Phe Glu Trp Gly Val Leu Phe Gly Phe Gly 
        355                 360                 365             


Pro Gly Leu Thr Val Glu Arg Val Val Val Arg Ser Val Pro Ile Lys 
    370                 375                 380                 


Tyr 
385 


<210>  3
<211>  387
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial TKS

<400>  3

Met Asn His Leu Arg Ala Glu Gly Pro Ala Ser Val Leu Ala Ile Gly 
1               5                   10                  15      


Thr Ala Asn Pro Glu Asn Ile Leu Leu Gln Asp Glu Phe Pro Asp Tyr 
            20                  25                  30          


Tyr Phe Arg Val Thr Lys Ser Glu His Met Thr Gln Leu Lys Glu Lys 
        35                  40                  45              


Phe Arg Lys Ile Cys Asp Lys Ser Met Ile Arg Lys Arg Asn Cys Phe 
    50                  55                  60                  


Leu Asn Glu Glu His Leu Lys Gln Asn Pro Arg Leu Val Glu His Glu 
65                  70                  75                  80  


Met Gln Thr Leu Asp Ala Arg Gln Asp Met Leu Val Val Glu Val Pro 
                85                  90                  95      


Lys Leu Gly Lys Asp Ala Cys Ala Lys Ala Ile Lys Glu Trp Gly Gln 
            100                 105                 110         


Pro Lys Ser Lys Ile Thr His Leu Ile Phe Thr Ser Ala Ser Thr Thr 
        115                 120                 125             


Asp Met Pro Gly Ala Asp Tyr His Cys Ala Lys Leu Leu Gly Leu Ser 
    130                 135                 140                 


Pro Ser Val Lys Arg Val Met Met Tyr Gln Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 


Gly Thr Val Leu Arg Ile Ala Lys Asp Ile Ala Glu Asn Asn Lys Gly 
                165                 170                 175     


Ala Arg Val Leu Ala Val Cys Cys Asp Ile Met Ala Cys Leu Phe Arg 
            180                 185                 190         


Gly Pro Ser Glu Ser Asp Leu Glu Leu Leu Val Gly Gln Ala Ile Phe 
        195                 200                 205             


Gly Asp Gly Ala Ala Ala Val Ile Val Gly Ala Glu Pro Asp Glu Ser 
    210                 215                 220                 


Val Gly Glu Arg Pro Ile Phe Glu Leu Val Ser Thr Gly Gln Thr Ile 
225                 230                 235                 240 


Leu Pro Asn Ser Glu Gly Thr Ile Gly Gly His Ile Arg Glu Ala Gly 
                245                 250                 255     


Leu Ile Phe Asp Leu His Lys Asp Val Pro Met Leu Ile Ser Asn Asn 
            260                 265                 270         


Ile Glu Lys Cys Leu Ile Glu Ala Phe Thr Pro Ile Gly Ile Ser Asp 
        275                 280                 285             


Trp Asn Ser Ile Phe Trp Ile Thr His Pro Gly Gly Lys Ala Ile Leu 
    290                 295                 300                 


Asp Lys Val Glu Glu Lys Leu His Leu Lys Ser Asp Lys Phe Val Asp 
305                 310                 315                 320 


Ser Arg His Val Leu Ser Glu His Gly Asn Met Ser Ser Ser Thr Val 
                325                 330                 335     


Leu Phe Val Met Asp Glu Leu Arg Lys Arg Ser Leu Glu Glu Gly Lys 
            340                 345                 350         


Ser Thr Thr Gly Asp Gly Phe Glu Trp Gly Val Leu Phe Gly Phe Gly 
        355                 360                 365             


Pro Gly Leu Thr Val Glu Arg Val Val Val Arg Ser Val Pro Ile Lys 
    370                 375                 380                 


Tyr Gly Ser 
385         


<210>  4
<211>  395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CsPT1 UniProt ID A0A455ZIK6

<400>  4

Met Gly Leu Ser Ser Val Cys Thr Phe Ser Phe Gln Thr Asn Tyr His 
1               5                   10                  15      


Thr Leu Leu Asn Pro His Asn Asn Asn Pro Lys Thr Ser Leu Leu Cys 
            20                  25                  30          


Tyr Arg His Pro Lys Thr Pro Ile Lys Tyr Ser Tyr Asn Asn Phe Pro 
        35                  40                  45              


Ser Lys His Cys Ser Thr Lys Ser Phe His Leu Gln Asn Lys Cys Ser 
    50                  55                  60                  


Glu Ser Leu Ser Ile Ala Lys Asn Ser Ile Arg Ala Ala Thr Thr Asn 
65                  70                  75                  80  


Gln Thr Glu Pro Pro Glu Ser Asp Asn His Ser Val Ala Thr Lys Ile 
                85                  90                  95      


Leu Asn Phe Gly Lys Ala Cys Trp Lys Leu Gln Arg Pro Tyr Thr Ile 
            100                 105                 110         


Ile Ala Phe Thr Ser Cys Ala Cys Gly Leu Phe Gly Lys Glu Leu Leu 
        115                 120                 125             


His Asn Thr Asn Leu Ile Ser Trp Ser Leu Met Phe Lys Ala Phe Phe 
    130                 135                 140                 


Phe Leu Val Ala Ile Leu Cys Ile Ala Ser Phe Thr Thr Thr Ile Asn 
145                 150                 155                 160 


Gln Ile Tyr Asp Leu His Ile Asp Arg Ile Asn Lys Pro Asp Leu Pro 
                165                 170                 175     


Leu Ala Ser Gly Glu Ile Ser Val Asn Thr Ala Trp Ile Met Ser Ile 
            180                 185                 190         


Ile Val Ala Leu Phe Gly Leu Ile Ile Thr Ile Lys Met Lys Gly Gly 
        195                 200                 205             


Pro Leu Tyr Ile Phe Gly Tyr Cys Phe Gly Ile Phe Gly Gly Ile Val 
    210                 215                 220                 


Tyr Ser Val Pro Pro Phe Arg Trp Lys Gln Asn Pro Ser Thr Ala Phe 
225                 230                 235                 240 


Leu Leu Asn Phe Leu Ala His Ile Ile Thr Asn Phe Thr Phe Tyr Tyr 
                245                 250                 255     


Ala Ser Arg Ala Ala Leu Gly Leu Pro Phe Glu Leu Arg Pro Ser Phe 
            260                 265                 270         


Thr Phe Leu Leu Ala Phe Met Lys Ser Met Gly Ser Ala Leu Ala Leu 
        275                 280                 285             


Ile Lys Asp Ala Ser Asp Val Glu Gly Asp Thr Lys Phe Gly Ile Ser 
    290                 295                 300                 


Thr Leu Ala Ser Lys Tyr Gly Ser Arg Asn Leu Thr Leu Phe Cys Ser 
305                 310                 315                 320 


Gly Ile Val Leu Leu Ser Tyr Val Ala Ala Ile Leu Ala Gly Ile Ile 
                325                 330                 335     


Trp Pro Gln Ala Phe Asn Ser Asn Val Met Leu Leu Ser His Ala Ile 
            340                 345                 350         


Leu Ala Phe Trp Leu Ile Leu Gln Thr Arg Asp Phe Ala Leu Thr Asn 
        355                 360                 365             


Tyr Asp Pro Glu Ala Gly Arg Arg Phe Tyr Glu Phe Met Trp Lys Leu 
    370                 375                 380                 


Tyr Tyr Ala Glu Tyr Leu Val Tyr Val Phe Ile 
385                 390                 395 


<210>  5
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CsPT4 UniProt ID A0A455ZJC3


<220>
<221>  VARIANT
<222>  (399)..(402)
<223>  Xaa can be any naturally occurring amino acid or absent

<400>  5

Met Gly Leu Ser Leu Val Cys Thr Phe Ser Phe Gln Thr Asn Tyr His 
1               5                   10                  15      


Thr Leu Leu Asn Pro His Asn Lys Asn Pro Lys Asn Ser Leu Leu Ser 
            20                  25                  30          


Tyr Gln His Pro Lys Thr Pro Ile Ile Lys Ser Ser Tyr Asp Asn Phe 
        35                  40                  45              


Pro Ser Lys Tyr Cys Leu Thr Lys Asn Phe His Leu Leu Gly Leu Asn 
    50                  55                  60                  


Ser His Asn Arg Ile Ser Ser Gln Ser Arg Ser Ile Arg Ala Gly Ser 
65                  70                  75                  80  


Asp Gln Ile Glu Gly Ser Pro His His Glu Ser Asp Asn Ser Ile Ala 
                85                  90                  95      


Thr Lys Ile Leu Asn Phe Gly His Thr Cys Trp Lys Leu Gln Arg Pro 
            100                 105                 110         


Tyr Val Val Lys Gly Met Ile Ser Ile Ala Cys Gly Leu Phe Gly Arg 
        115                 120                 125             


Glu Leu Phe Asn Asn Arg His Leu Phe Ser Trp Gly Leu Met Trp Lys 
    130                 135                 140                 


Ala Phe Phe Ala Leu Val Pro Ile Leu Ser Phe Asn Phe Phe Ala Ala 
145                 150                 155                 160 


Ile Met Asn Gln Ile Tyr Asp Val Asp Ile Asp Arg Ile Asn Lys Pro 
                165                 170                 175     


Asp Leu Pro Leu Val Ser Gly Glu Met Ser Ile Glu Thr Ala Trp Ile 
            180                 185                 190         


Leu Ser Ile Ile Val Ala Leu Thr Gly Leu Ile Val Thr Ile Lys Leu 
        195                 200                 205             


Lys Ser Ala Pro Leu Phe Val Phe Ile Tyr Ile Phe Gly Ile Phe Ala 
    210                 215                 220                 


Gly Phe Ala Tyr Ser Val Pro Pro Ile Arg Trp Lys Gln Tyr Pro Phe 
225                 230                 235                 240 


Thr Asn Phe Leu Ile Thr Ile Ser Ser His Val Gly Leu Ala Phe Thr 
                245                 250                 255     


Ser Tyr Ser Ala Thr Thr Ser Ala Leu Gly Leu Pro Phe Val Trp Arg 
            260                 265                 270         


Pro Ala Phe Ser Phe Ile Ile Ala Phe Met Thr Val Met Gly Met Thr 
        275                 280                 285             


Ile Ala Phe Ala Lys Asp Ile Ser Asp Ile Glu Gly Asp Ala Lys Tyr 
    290                 295                 300                 


Gly Val Ser Thr Val Ala Thr Lys Leu Gly Ala Arg Asn Met Thr Phe 
305                 310                 315                 320 


Val Val Ser Gly Val Leu Leu Leu Asn Tyr Leu Val Ser Ile Ser Ile 
                325                 330                 335     


Gly Ile Ile Trp Pro Gln Val Phe Lys Ser Asn Ile Met Ile Leu Ser 
            340                 345                 350         


His Ala Ile Leu Ala Phe Cys Leu Ile Phe Gln Thr Arg Glu Leu Ala 
        355                 360                 365             


Leu Ala Asn Tyr Ala Ser Ala Pro Ser Arg Gln Phe Phe Glu Phe Ile 
    370                 375                 380                 


Trp Leu Leu Tyr Tyr Ala Glu Tyr Phe Val Tyr Val Phe Ile Xaa Xaa 
385                 390                 395                 400 


Xaa Xaa 
        


<210>  6
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CsPT4t. A truncated CsPT1 minus the N-terminal 1-76 amino acids 
       of CsPT1


<220>
<221>  VARIANT
<222>  (324)..(327)
<223>  Xaa can be any naturally occuring amino acid or absent

<400>  6

Met Ser Ala Gly Ser Asp Gln Ile Glu Gly Ser Pro His His Glu Ser 
1               5                   10                  15      


Asp Asn Ser Ile Ala Thr Lys Ile Leu Asn Phe Gly His Thr Cys Trp 
            20                  25                  30          


Lys Leu Gln Arg Pro Tyr Val Val Lys Gly Met Ile Ser Ile Ala Cys 
        35                  40                  45              


Gly Leu Phe Gly Arg Glu Leu Phe Asn Asn Arg His Leu Phe Ser Trp 
    50                  55                  60                  


Gly Leu Met Trp Lys Ala Phe Phe Ala Leu Val Pro Ile Leu Ser Phe 
65                  70                  75                  80  


Asn Phe Phe Ala Ala Ile Met Asn Gln Ile Tyr Asp Val Asp Ile Asp 
                85                  90                  95      


Arg Ile Asn Lys Pro Asp Leu Pro Leu Val Ser Gly Glu Met Ser Ile 
            100                 105                 110         


Glu Thr Ala Trp Ile Leu Ser Ile Ile Val Ala Leu Thr Gly Leu Ile 
        115                 120                 125             


Val Thr Ile Lys Leu Lys Ser Ala Pro Leu Phe Val Phe Ile Tyr Ile 
    130                 135                 140                 


Phe Gly Ile Phe Ala Gly Phe Ala Tyr Ser Val Pro Pro Ile Arg Trp 
145                 150                 155                 160 


Lys Gln Tyr Pro Phe Thr Asn Phe Leu Ile Thr Ile Ser Ser His Val 
                165                 170                 175     


Gly Leu Ala Phe Thr Ser Tyr Ser Ala Thr Thr Ser Ala Leu Gly Leu 
            180                 185                 190         


Pro Phe Val Trp Arg Pro Ala Phe Ser Phe Ile Ile Ala Phe Met Thr 
        195                 200                 205             


Val Met Gly Met Thr Ile Ala Phe Ala Lys Asp Ile Ser Asp Ile Glu 
    210                 215                 220                 


Gly Asp Ala Lys Tyr Gly Val Ser Thr Val Ala Thr Lys Leu Gly Ala 
225                 230                 235                 240 


Arg Asn Met Thr Phe Val Val Ser Gly Val Leu Leu Leu Asn Tyr Leu 
                245                 250                 255     


Val Ser Ile Ser Ile Gly Ile Ile Trp Pro Gln Val Phe Lys Ser Asn 
            260                 265                 270         


Ile Met Ile Leu Ser His Ala Ile Leu Ala Phe Cys Leu Ile Phe Gln 
        275                 280                 285             


Thr Arg Glu Leu Ala Leu Ala Asn Tyr Ala Ser Ala Pro Ser Arg Gln 
    290                 295                 300                 


Phe Phe Glu Phe Ile Trp Leu Leu Tyr Tyr Ala Glu Tyr Phe Val Tyr 
305                 310                 315                 320 


Val Phe Ile Xaa Xaa Xaa Xaa 
                325         


<210>  7
<211>  101
<212>  PRT
<213>  Cannabis sativa

<400>  7

Met Ala Val Lys His Leu Ile Val Leu Lys Phe Lys Asp Glu Ile Thr 
1               5                   10                  15      


Glu Ala Gln Lys Glu Glu Phe Phe Lys Thr Tyr Val Asn Leu Val Asn 
            20                  25                  30          


Ile Ile Pro Ala Met Lys Asp Val Tyr Trp Gly Lys Asp Val Thr Gln 
        35                  40                  45              


Lys Asn Lys Glu Glu Gly Tyr Thr His Ile Val Glu Val Thr Phe Glu 
    50                  55                  60                  


Ser Val Glu Thr Ile Gln Asp Tyr Ile Ile His Pro Ala His Val Gly 
65                  70                  75                  80  


Phe Gly Asp Val Tyr Arg Ser Phe Trp Glu Lys Leu Leu Ile Phe Asp 
                85                  90                  95      


Tyr Thr Pro Arg Lys 
            100     


<210>  8
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial olivetolic acid cyclase

<400>  8

Met Ala Val Lys His Leu Ile Val Leu Lys Phe Lys Asp Glu Ile Thr 
1               5                   10                  15      


Glu Ala Gln Lys Glu Glu Phe Phe Lys Thr Tyr Val Asn Leu Val Asn 
            20                  25                  30          


Ile Ile Pro Ala Met Lys Asp Val Tyr Trp Gly Lys Asp Val Thr Gln 
        35                  40                  45              


Lys Asn Lys Glu Glu Gly Tyr Thr His Ile Val Glu Val Thr Phe Glu 
    50                  55                  60                  


Ser Val Glu Thr Ile Gln Asp Tyr Ile Ile His Pro Ala His Val Gly 
65                  70                  75                  80  


Phe Gly Asp Val Tyr Arg Ser Phe Trp Glu Lys Leu Leu Ile Phe Asp 
                85                  90                  95      


Tyr Thr Pro Arg Lys Gly Ser 
            100             


<210>  9
<211>  720
<212>  PRT
<213>  Cannabis sativa

<400>  9

Met Gly Lys Asn Tyr Lys Ser Leu Asp Ser Val Val Ala Ser Asp Phe 
1               5                   10                  15      


Ile Ala Leu Gly Ile Thr Ser Glu Val Ala Glu Thr Leu His Gly Arg 
            20                  25                  30          


Leu Ala Glu Ile Val Cys Asn Tyr Gly Ala Ala Thr Pro Gln Thr Trp 
        35                  40                  45              


Ile Asn Ile Ala Asn His Ile Leu Ser Pro Asp Leu Pro Phe Ser Leu 
    50                  55                  60                  


His Gln Met Leu Phe Tyr Gly Cys Tyr Lys Asp Phe Gly Pro Ala Pro 
65                  70                  75                  80  


Pro Ala Trp Ile Pro Asp Pro Glu Lys Val Lys Ser Thr Asn Leu Gly 
                85                  90                  95      


Ala Leu Leu Glu Lys Arg Gly Lys Glu Phe Leu Gly Val Lys Tyr Lys 
            100                 105                 110         


Asp Pro Ile Ser Ser Phe Ser His Phe Gln Glu Phe Ser Val Arg Asn 
        115                 120                 125             


Pro Glu Val Tyr Trp Arg Thr Val Leu Met Asp Glu Met Lys Ile Ser 
    130                 135                 140                 


Phe Ser Lys Asp Pro Glu Cys Ile Leu Arg Arg Asp Asp Ile Asn Asn 
145                 150                 155                 160 


Pro Gly Gly Ser Glu Trp Leu Pro Gly Gly Tyr Leu Asn Ser Ala Lys 
                165                 170                 175     


Asn Cys Leu Asn Val Asn Ser Asn Lys Lys Leu Asn Asp Thr Met Ile 
            180                 185                 190         


Val Trp Arg Asp Glu Gly Asn Asp Asp Leu Pro Leu Asn Lys Leu Thr 
        195                 200                 205             


Leu Asp Gln Leu Arg Lys Arg Val Trp Leu Val Gly Tyr Ala Leu Glu 
    210                 215                 220                 


Glu Met Gly Leu Glu Lys Gly Cys Ala Ile Ala Ile Asp Met Pro Met 
225                 230                 235                 240 


His Val Asp Ala Val Val Ile Tyr Leu Ala Ile Val Leu Ala Gly Tyr 
                245                 250                 255     


Val Val Val Ser Ile Ala Asp Ser Phe Ser Ala Pro Glu Ile Ser Thr 
            260                 265                 270         


Arg Leu Arg Leu Ser Lys Ala Lys Ala Ile Phe Thr Gln Asp His Ile 
        275                 280                 285             


Ile Arg Gly Lys Lys Arg Ile Pro Leu Tyr Ser Arg Val Val Glu Ala 
    290                 295                 300                 


Lys Ser Pro Met Ala Ile Val Ile Pro Cys Ser Gly Ser Asn Ile Gly 
305                 310                 315                 320 


Ala Glu Leu Arg Asp Gly Asp Ile Ser Trp Asp Tyr Phe Leu Glu Arg 
                325                 330                 335     


Ala Lys Glu Phe Lys Asn Cys Glu Phe Thr Ala Arg Glu Gln Pro Val 
            340                 345                 350         


Asp Ala Tyr Thr Asn Ile Leu Phe Ser Ser Gly Thr Thr Gly Glu Pro 
        355                 360                 365             


Lys Ala Ile Pro Trp Thr Gln Ala Thr Pro Leu Lys Ala Ala Ala Asp 
    370                 375                 380                 


Gly Trp Ser His Leu Asp Ile Arg Lys Gly Asp Val Ile Val Trp Pro 
385                 390                 395                 400 


Thr Asn Leu Gly Trp Met Met Gly Pro Trp Leu Val Tyr Ala Ser Leu 
                405                 410                 415     


Leu Asn Gly Ala Ser Ile Ala Leu Tyr Asn Gly Ser Pro Leu Val Ser 
            420                 425                 430         


Gly Phe Ala Lys Phe Val Gln Asp Ala Lys Val Thr Met Leu Gly Val 
        435                 440                 445             


Val Pro Ser Ile Val Arg Ser Trp Lys Ser Thr Asn Cys Val Ser Gly 
    450                 455                 460                 


Tyr Asp Trp Ser Thr Ile Arg Cys Phe Ser Ser Ser Gly Glu Ala Ser 
465                 470                 475                 480 


Asn Val Asp Glu Tyr Leu Trp Leu Met Gly Arg Ala Asn Tyr Lys Pro 
                485                 490                 495     


Val Ile Glu Met Cys Gly Gly Thr Glu Ile Gly Gly Ala Phe Ser Ala 
            500                 505                 510         


Gly Ser Phe Leu Gln Ala Gln Ser Leu Ser Ser Phe Ser Ser Gln Cys 
        515                 520                 525             


Met Gly Cys Thr Leu Tyr Ile Leu Asp Lys Asn Gly Tyr Pro Met Pro 
    530                 535                 540                 


Lys Asn Lys Pro Gly Ile Gly Glu Leu Ala Leu Gly Pro Val Met Phe 
545                 550                 555                 560 


Gly Ala Ser Lys Thr Leu Leu Asn Gly Asn His His Asp Val Tyr Phe 
                565                 570                 575     


Lys Gly Met Pro Thr Leu Asn Gly Glu Val Leu Arg Arg His Gly Asp 
            580                 585                 590         


Ile Phe Glu Leu Thr Ser Asn Gly Tyr Tyr His Ala His Gly Arg Ala 
        595                 600                 605             


Asp Asp Thr Met Asn Ile Gly Gly Ile Lys Ile Ser Ser Ile Glu Ile 
    610                 615                 620                 


Glu Arg Val Cys Asn Glu Val Asp Asp Arg Val Phe Glu Thr Thr Ala 
625                 630                 635                 640 


Ile Gly Val Pro Pro Leu Gly Gly Gly Pro Glu Gln Leu Val Ile Phe 
                645                 650                 655     


Phe Val Leu Lys Asp Ser Asn Asp Thr Thr Ile Asp Leu Asn Gln Leu 
            660                 665                 670         


Arg Leu Ser Phe Asn Leu Gly Leu Gln Lys Lys Leu Asn Pro Leu Phe 
        675                 680                 685             


Lys Val Thr Arg Val Val Pro Leu Ser Ser Leu Pro Arg Thr Ala Thr 
    690                 695                 700                 


Asn Lys Ile Met Arg Arg Val Leu Arg Gln Gln Phe Ser His Phe Glu 
705                 710                 715                 720 


<210>  10
<211>  533
<212>  PRT
<213>  Cannabis sativa

<400>  10

Met Glu Lys Ser Gly Tyr Gly Arg Asp Gly Ile Tyr Arg Ser Leu Arg 
1               5                   10                  15      


Pro Pro Leu His Leu Pro Asn Asn Asn Asn Leu Ser Met Val Ser Phe 
            20                  25                  30          


Leu Phe Arg Asn Ser Ser Ser Tyr Pro Gln Lys Pro Ala Leu Ile Asp 
        35                  40                  45              


Ser Glu Thr Asn Gln Ile Leu Ser Phe Ser His Phe Lys Ser Thr Val 
    50                  55                  60                  


Ile Lys Val Ser His Gly Phe Leu Asn Leu Gly Ile Lys Lys Asn Asp 
65                  70                  75                  80  


Trp Leu Ile Tyr Ala Pro Asn Ser Ile His Phe Pro Val Cys Phe Leu 
                85                  90                  95      


Gly Ile Ile Ala Ser Gly Ala Ile Ala Thr Thr Ser Asn Pro Leu Tyr 
            100                 105                 110         


Thr Val Ser Glu Leu Ser Lys Gln Val Lys Asp Ser Asn Pro Lys Leu 
        115                 120                 125             


Ile Ile Thr Val Pro Gln Leu Leu Glu Lys Val Lys Gly Phe Asn Leu 
    130                 135                 140                 


Pro Thr Ile Leu Ile Gly Pro Asp Ser Glu Gln Glu Ser Ser Ser Asp 
145                 150                 155                 160 


Lys Val Met Thr Phe Asn Asp Leu Val Asn Leu Gly Gly Ser Ser Gly 
                165                 170                 175     


Ser Glu Phe Pro Ile Val Asp Asp Phe Lys Gln Ser Asp Thr Ala Ala 
            180                 185                 190         


Leu Leu Tyr Ser Ser Gly Thr Thr Gly Met Ser Lys Gly Trp Leu Thr 
        195                 200                 205             


His Lys Asn Phe Ile Ala Ser Ser Leu Met Val Thr Met Glu Gln Asp 
    210                 215                 220                 


Leu Val Gly Glu Met Asp Asn Val Phe Leu Cys Phe Leu Pro Met Phe 
225                 230                 235                 240 


His Val Phe Gly Leu Ala Ile Ile Thr Tyr Ala Gln Leu Gln Arg Gly 
                245                 250                 255     


Asn Thr Val Ile Ser Ala Arg Phe Asp Leu Glu Lys Met Leu Lys Asp 
            260                 265                 270         


Val Glu Lys Tyr Val Thr His Leu Trp Trp Pro Pro Val Ile Leu Ala 
        275                 280                 285             


Leu Ser Lys Asn Ser Met Val Lys Phe Asn Leu Ser Ser Ile Lys Tyr 
    290                 295                 300                 


Ile Gly Ser Gly Ala Ala Pro Leu Gly Lys Asp Leu Met Glu Glu Cys 
305                 310                 315                 320 


Ser Lys Trp Pro Tyr Gly Ile Val Ala Gln Gly Tyr Gly Met Thr Glu 
                325                 330                 335     


Thr Cys Gly Ile Val Ser Met Glu Asp Ile Arg Gly Gly Lys Arg Asn 
            340                 345                 350         


Ser Gly Ser Ala Gly Met Leu Ala Ser Gly Val Glu Ala Gln Ile Val 
        355                 360                 365             


Ser Val Asp Thr Leu Lys Pro Leu Pro Pro Asn Gln Leu Gly Glu Ile 
    370                 375                 380                 


Trp Val Lys Gly Pro Asn Met Met Gln Gly Tyr Phe Asn Asn Pro Gln 
385                 390                 395                 400 


Ala Thr Lys Leu Thr Ile Asp Lys Lys Gly Trp Val His Thr Gly Asp 
                405                 410                 415     


Leu Gly Tyr Phe Asp Glu Asp Gly His Leu Tyr Trp Asp Arg Ile Lys 
            420                 425                 430         


Glu Leu Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala Glu Leu Glu 
        435                 440                 445             


Gly Leu Leu Val Ser His Pro Glu Ile Leu Asp Ala Trp Ile Pro Phe 
    450                 455                 460                 


Pro Asp Ala Glu Ala Gly Glu Val Pro Val Ala Tyr Trp Arg Ser Pro 
465                 470                 475                 480 


Asn Ser Ser Leu Thr Glu Asn Asp Val Lys Lys Phe Ile Ala Gly Gln 
                485                 490                 495     


Val Ala Ser Phe Lys Arg Leu Arg Lys Val Thr Phe Ile Asn Ser Val 
            500                 505                 510         


Pro Lys Ser Ala Ser Gly Lys Ile Leu Arg Arg Glu Leu Ile Gln Lys 
        515                 520                 525             


Val Arg Ser Asn Met 
    530             


<210>  11
<211>  521
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated Cannabis sativa acyl activating enzyme

<400>  11

Met Glu Lys Ser Gly Tyr Gly Arg Asp Gly Ile Tyr Arg Ser Leu Arg 
1               5                   10                  15      


Pro Pro Leu His Leu Pro Asn Asn Asn Asn Leu Ser Met Val Ser Phe 
            20                  25                  30          


Leu Phe Arg Asn Ser Ser Ser Tyr Pro Gln Lys Pro Ala Leu Ile Asp 
        35                  40                  45              


Ser Glu Thr Asn Gln Ile Leu Ser Phe Ser His Phe Lys Ser Thr Val 
    50                  55                  60                  


Ile Lys Val Ser His Gly Phe Leu Asn Leu Gly Ile Lys Lys Asn Asp 
65                  70                  75                  80  


Trp Leu Ile Tyr Ala Pro Asn Ser Ile His Phe Pro Val Cys Phe Leu 
                85                  90                  95      


Gly Ile Ile Ala Ser Gly Ala Ile Ala Thr Thr Ser Asn Pro Leu Tyr 
            100                 105                 110         


Thr Val Ser Glu Leu Ser Lys Gln Val Lys Asp Ser Asn Pro Lys Leu 
        115                 120                 125             


Ile Ile Thr Val Pro Gln Leu Leu Glu Lys Val Lys Gly Phe Asn Leu 
    130                 135                 140                 


Pro Thr Ile Leu Ile Gly Pro Asp Ser Glu Gln Glu Ser Ser Ser Asp 
145                 150                 155                 160 


Lys Val Met Thr Phe Asn Asp Leu Val Asn Leu Gly Gly Ser Ser Gly 
                165                 170                 175     


Ser Glu Phe Pro Ile Val Asp Asp Phe Lys Gln Ser Asp Thr Ala Ala 
            180                 185                 190         


Leu Leu Tyr Ser Ser Gly Thr Thr Gly Met Ser Lys Gly Trp Leu Thr 
        195                 200                 205             


His Lys Asn Phe Ile Ala Ser Ser Leu Met Val Thr Met Glu Gln Asp 
    210                 215                 220                 


Leu Val Gly Glu Met Asp Asn Val Phe Leu Cys Phe Leu Pro Met Phe 
225                 230                 235                 240 


His Val Phe Gly Leu Ala Ile Ile Thr Tyr Ala Gln Leu Gln Arg Gly 
                245                 250                 255     


Asn Thr Val Ile Ser Ala Arg Phe Asp Leu Glu Lys Met Leu Lys Asp 
            260                 265                 270         


Val Glu Lys Tyr Val Thr His Leu Trp Trp Pro Pro Val Ile Leu Ala 
        275                 280                 285             


Leu Ser Lys Asn Ser Met Val Lys Phe Asn Leu Ser Ser Ile Lys Tyr 
    290                 295                 300                 


Ile Gly Ser Gly Ala Ala Pro Leu Gly Lys Asp Leu Met Glu Glu Cys 
305                 310                 315                 320 


Ser Lys Trp Pro Tyr Gly Ile Val Ala Gln Gly Tyr Gly Met Thr Glu 
                325                 330                 335     


Thr Cys Gly Ile Val Ser Met Glu Asp Ile Arg Gly Gly Lys Arg Asn 
            340                 345                 350         


Ser Gly Ser Ala Gly Met Leu Ala Ser Gly Val Glu Ala Gln Ile Val 
        355                 360                 365             


Ser Val Asp Thr Leu Lys Pro Leu Pro Pro Asn Gln Leu Gly Glu Ile 
    370                 375                 380                 


Trp Val Lys Gly Pro Asn Met Met Gln Gly Tyr Phe Asn Asn Pro Gln 
385                 390                 395                 400 


Ala Thr Lys Leu Thr Ile Asp Lys Lys Gly Trp Val His Thr Gly Asp 
                405                 410                 415     


Leu Gly Tyr Phe Asp Glu Asp Gly His Leu Tyr Trp Asp Arg Ile Lys 
            420                 425                 430         


Glu Leu Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala Glu Leu Glu 
        435                 440                 445             


Gly Leu Leu Val Ser His Pro Glu Ile Leu Asp Ala Trp Ile Pro Phe 
    450                 455                 460                 


Pro Asp Ala Glu Ala Gly Glu Val Pro Val Ala Tyr Trp Arg Ser Pro 
465                 470                 475                 480 


Asn Ser Ser Leu Thr Glu Asn Asp Val Lys Lys Phe Ile Ala Gly Gln 
                485                 490                 495     


Val Ala Ser Phe Lys Arg Leu Arg Lys Val Thr Phe Ile Asn Ser Val 
            500                 505                 510         


Pro Lys Ser Ala Ser Gly Lys Ile Leu 
        515                 520     


<210>  12
<211>  566
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant medium chain fatty acid CoA ligase

<400>  12

Met His Pro Thr Gly Pro His Leu Gly Pro Asp Val Leu Phe Arg Glu 
1               5                   10                  15      


Ser Asn Met Lys Val Thr Leu Thr Phe Asn Glu Gln Arg Arg Ala Ala 
            20                  25                  30          


Tyr Arg Gln Gln Gly Leu Trp Gly Asp Ala Ser Leu Ala Asp Tyr Trp 
        35                  40                  45              


Gln Gln Thr Ala Arg Ala Met Pro Asp Lys Ile Ala Val Val Asp Asn 
    50                  55                  60                  


His Gly Ala Ser Tyr Thr Tyr Ser Ala Leu Asp His Ala Ala Ser Cys 
65                  70                  75                  80  


Leu Ala Asn Trp Met Leu Ala Lys Gly Ile Glu Ser Gly Asp Arg Ile 
                85                  90                  95      


Ala Phe Gln Leu Pro Gly Trp Cys Glu Phe Arg Val Ile Tyr Leu Ala 
            100                 105                 110         


Cys Leu Lys Ile Gly Ala Val Ser Val Pro Leu Leu Pro Ser Trp Arg 
        115                 120                 125             


Glu Ala Glu Leu Val Trp Val Leu Asn Lys Cys Gln Ala Lys Met Phe 
    130                 135                 140                 


Phe Ala Pro Thr Leu Phe Lys Gln Thr Arg Pro Val Asp Leu Ile Leu 
145                 150                 155                 160 


Pro Leu Gln Asn Gln Leu Pro Gln Leu Gln Gln Ile Val Gly Val Asp 
                165                 170                 175     


Lys Leu Ala Pro Ala Thr Ser Ser Leu Ser Leu Ser Gln Ile Ile Ala 
            180                 185                 190         


Asp Asn Thr Ser Leu Thr Thr Ala Ile Thr Thr His Gly Asp Glu Leu 
        195                 200                 205             


Ala Ala Val Leu Phe Thr Ser Gly Thr Glu Gly Leu Pro Lys Gly Val 
    210                 215                 220                 


Met Leu Thr His Asn Asn Ile Leu Ala Ser Glu Arg Ala Tyr Cys Ala 
225                 230                 235                 240 


Arg Leu Asn Leu Thr Trp Gln Asp Val Phe Met Met Pro Ala Pro Leu 
                245                 250                 255     


Gly His Ala Thr Gly Phe Leu His Gly Val Thr Ala Pro Phe Leu Ile 
            260                 265                 270         


Gly Ala Arg Ser Val Leu Leu Asp Ile Phe Phe Pro Asp Ala Cys Leu 
        275                 280                 285             


Ala Leu Leu Glu Gln Gln Arg Cys Thr Cys Met Leu Gly Ala Thr Pro 
    290                 295                 300                 


Phe Val Tyr Asp Leu Leu Asn Val Leu Glu Lys Gln Pro Ala Asp Leu 
305                 310                 315                 320 


Ser Ala Leu Arg Phe Phe Leu Cys Gly Gly Thr Thr Ile Pro Lys Lys 
                325                 330                 335     


Val Ala Arg Glu Cys Gln Gln Arg Gly Ile Lys Leu Leu Ser Val Tyr 
            340                 345                 350         


Gly Ser Thr Glu Ser Ser Pro His Ala Val Val Asn Leu Asp Asp Pro 
        355                 360                 365             


Leu Ser Arg Phe Met His Thr Asp Gly Tyr Ala Ala Ala Gly Val Glu 
    370                 375                 380                 


Ile Lys Val Val Asp Asp Ala Arg Lys Thr Leu Pro Pro Gly Cys Glu 
385                 390                 395                 400 


Gly Glu Glu Ala Ser Arg Gly Pro Asn Val Phe Met Gly Tyr Phe Asp 
                405                 410                 415     


Glu Pro Glu Leu Thr Ala Arg Ala Leu Asp Glu Glu Gly Trp Tyr Tyr 
            420                 425                 430         


Ser Gly Asp Leu Cys Arg Met Asp Glu Ala Gly Tyr Ile Lys Ile Thr 
        435                 440                 445             


Gly Arg Lys Lys Asp Ile Ile Val Arg Gly Gly Glu Asn Ile Ser Ser 
    450                 455                 460                 


Arg Glu Val Glu Asp Ile Leu Leu Gln His Pro Lys Ile His Asp Ala 
465                 470                 475                 480 


Cys Val Val Ala Met Ser Asp Glu Arg Leu Gly Glu Arg Ser Cys Ala 
                485                 490                 495     


Tyr Val Val Leu Lys Ala Pro His His Ser Leu Ser Leu Glu Glu Val 
            500                 505                 510         


Val Ala Phe Phe Ser Arg Lys Arg Val Ala Lys Tyr Lys Tyr Pro Glu 
        515                 520                 525             


His Ile Val Val Ile Glu Lys Leu Pro Arg Thr Thr Ser Gly Lys Ile 
    530                 535                 540                 


Gln Lys Phe Leu Leu Arg Lys Asp Ile Met Arg Arg Leu Thr Gln Asp 
545                 550                 555                 560 


Val Cys Glu Glu Ile Glu 
                565     


<210>  13
<211>  566
<212>  PRT
<213>  Escherichia coli

<400>  13

Met His Pro Thr Gly Pro His Leu Gly Pro Asp Val Leu Phe Arg Glu 
1               5                   10                  15      


Ser Asn Met Lys Val Thr Leu Thr Phe Asn Glu Gln Arg Arg Ala Ala 
            20                  25                  30          


Tyr Arg Gln Gln Gly Leu Trp Gly Asp Ala Ser Leu Ala Asp Tyr Trp 
        35                  40                  45              


Gln Gln Thr Ala Arg Ala Met Pro Asp Lys Ile Ala Val Val Asp Asn 
    50                  55                  60                  


His Gly Ala Ser Tyr Thr Tyr Ser Ala Leu Asp His Ala Ala Ser Cys 
65                  70                  75                  80  


Leu Ala Asn Trp Met Leu Ala Lys Gly Ile Glu Ser Gly Asp Arg Ile 
                85                  90                  95      


Ala Phe Gln Leu Pro Gly Trp Cys Glu Phe Thr Val Ile Tyr Leu Ala 
            100                 105                 110         


Cys Leu Lys Ile Gly Ala Val Ser Val Pro Leu Leu Pro Ser Trp Arg 
        115                 120                 125             


Glu Ala Glu Leu Val Trp Val Leu Asn Lys Cys Gln Ala Lys Met Phe 
    130                 135                 140                 


Phe Ala Pro Thr Leu Phe Lys Gln Thr Arg Pro Val Asp Leu Ile Leu 
145                 150                 155                 160 


Pro Leu Gln Asn Gln Leu Pro Gln Leu Gln Gln Ile Val Gly Val Asp 
                165                 170                 175     


Lys Leu Ala Pro Ala Thr Ser Ser Leu Ser Leu Ser Gln Ile Ile Ala 
            180                 185                 190         


Asp Asn Thr Ser Leu Thr Thr Ala Ile Thr Thr His Gly Asp Glu Leu 
        195                 200                 205             


Ala Ala Val Leu Phe Thr Ser Gly Thr Glu Gly Leu Pro Lys Gly Val 
    210                 215                 220                 


Met Leu Thr His Asn Asn Ile Leu Ala Ser Glu Arg Ala Tyr Cys Ala 
225                 230                 235                 240 


Arg Leu Asn Leu Thr Trp Gln Asp Val Phe Met Met Pro Ala Pro Leu 
                245                 250                 255     


Gly His Ala Thr Gly Phe Leu His Gly Val Thr Ala Pro Phe Leu Ile 
            260                 265                 270         


Gly Ala Arg Ser Val Leu Leu Asp Ile Phe Thr Pro Asp Ala Cys Leu 
        275                 280                 285             


Ala Leu Leu Glu Gln Gln Arg Cys Thr Cys Met Leu Gly Ala Thr Pro 
    290                 295                 300                 


Phe Val Tyr Asp Leu Leu Asn Val Leu Glu Lys Gln Pro Ala Asp Leu 
305                 310                 315                 320 


Ser Ala Leu Arg Phe Phe Leu Cys Gly Gly Thr Thr Ile Pro Lys Lys 
                325                 330                 335     


Val Ala Arg Glu Cys Gln Gln Arg Gly Ile Lys Leu Leu Ser Val Tyr 
            340                 345                 350         


Gly Ser Thr Glu Ser Ser Pro His Ala Val Val Asn Leu Asp Asp Pro 
        355                 360                 365             


Leu Ser Arg Phe Met His Thr Asp Gly Tyr Ala Ala Ala Gly Val Glu 
    370                 375                 380                 


Ile Lys Val Val Asp Asp Ala Arg Lys Thr Leu Pro Pro Gly Cys Glu 
385                 390                 395                 400 


Gly Glu Glu Ala Ser Arg Gly Pro Asn Val Phe Met Gly Tyr Phe Asp 
                405                 410                 415     


Glu Pro Glu Leu Thr Ala Arg Ala Leu Asp Glu Glu Gly Trp Tyr Tyr 
            420                 425                 430         


Ser Gly Asp Leu Cys Arg Met Asp Glu Ala Gly Tyr Ile Lys Ile Thr 
        435                 440                 445             


Gly Arg Lys Lys Asp Ile Ile Val Arg Gly Gly Glu Asn Ile Ser Ser 
    450                 455                 460                 


Arg Glu Val Glu Asp Ile Leu Leu Gln His Pro Lys Ile His Asp Ala 
465                 470                 475                 480 


Cys Val Val Ala Met Ser Asp Glu Arg Leu Gly Glu Arg Ser Cys Ala 
                485                 490                 495     


Tyr Val Val Leu Lys Ala Pro His His Ser Leu Ser Leu Glu Glu Val 
            500                 505                 510         


Val Ala Phe Phe Ser Arg Lys Arg Val Ala Lys Tyr Lys Tyr Pro Glu 
        515                 520                 525             


His Ile Val Val Ile Glu Lys Leu Pro Arg Thr Thr Ser Gly Lys Ile 
    530                 535                 540                 


Gln Lys Phe Leu Leu Arg Lys Asp Ile Met Arg Arg Leu Thr Gln Asp 
545                 550                 555                 560 


Val Cys Glu Glu Ile Glu 
                565     


<210>  14
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Active site of wild type Cannabis sativa olivetol synthase amino 
       acid sequence

<400>  14

Asp Leu Glu Leu Leu Val Gly Gln Ala Ile Phe Gly 
1               5                   10          


<210>  15
<211>  566
<212>  PRT
<213>  Escherichia coli

<400>  15

Met His Pro Thr Gly Pro His Leu Gly Pro Asp Val Leu Phe Arg Glu 
1               5                   10                  15      


Ser Asn Met Lys Val Thr Leu Thr Phe Asn Glu Gln Arg Arg Ala Ala 
            20                  25                  30          


Tyr Arg Gln Gln Gly Leu Trp Gly Asp Ala Ser Leu Ala Asp Tyr Trp 
        35                  40                  45              


Gln Gln Thr Ala Arg Ala Met Pro Asp Lys Ile Ala Val Val Asp Asn 
    50                  55                  60                  


His Gly Ala Ser Tyr Thr Tyr Ser Ala Leu Asp His Ala Ala Ser Cys 
65                  70                  75                  80  


Leu Ala Asn Trp Met Leu Ala Lys Gly Ile Glu Ser Gly Asp Arg Ile 
                85                  90                  95      


Ala Phe Gln Leu Pro Gly Trp Cys Glu Phe Thr Val Ile Tyr Leu Ala 
            100                 105                 110         


Cys Leu Lys Ile Gly Ala Val Ser Val Pro Leu Leu Pro Ser Trp Arg 
        115                 120                 125             


Glu Ala Glu Leu Val Trp Val Leu Asn Lys Cys Gln Ala Lys Met Phe 
    130                 135                 140                 


Phe Ala Pro Thr Leu Phe Lys Gln Thr Arg Pro Val Asp Leu Ile Leu 
145                 150                 155                 160 


Pro Leu Gln Asn Gln Leu Pro Gln Leu Gln Gln Ile Val Gly Val Asp 
                165                 170                 175     


Lys Leu Ala Pro Ala Thr Ser Ser Leu Ser Leu Ser Gln Ile Ile Ala 
            180                 185                 190         


Asp Asn Thr Ser Leu Thr Thr Ala Ile Thr Thr His Gly Asp Glu Leu 
        195                 200                 205             


Ala Ala Val Leu Phe Thr Ser Gly Thr Glu Gly Leu Pro Lys Gly Val 
    210                 215                 220                 


Met Leu Thr His Asn Asn Ile Leu Ala Ser Glu Arg Ala Tyr Cys Ala 
225                 230                 235                 240 


Arg Leu Asn Leu Thr Trp Gln Asp Val Phe Met Met Pro Ala Pro Leu 
                245                 250                 255     


Gly His Ala Thr Gly Phe Leu His Gly Val Thr Ala Pro Phe Leu Ile 
            260                 265                 270         


Gly Ala Arg Ser Val Leu Leu Asp Ile Phe Thr Pro Asp Ala Cys Leu 
        275                 280                 285             


Ala Leu Leu Glu Gln Gln Arg Cys Thr Cys Met Leu Gly Ala Thr Pro 
    290                 295                 300                 


Phe Val Tyr Asp Leu Leu Asn Val Leu Glu Lys Gln Pro Ala Asp Leu 
305                 310                 315                 320 


Ser Ala Leu Arg Phe Phe Leu Cys Gly Gly Thr Thr Ile Pro Lys Lys 
                325                 330                 335     


Val Ala Arg Glu Cys Gln Gln Arg Gly Ile Lys Leu Leu Ser Val Tyr 
            340                 345                 350         


Gly Ser Thr Glu Ser Ser Pro His Ala Val Val Asn Leu Asp Asp Pro 
        355                 360                 365             


Leu Ser Arg Phe Met His Thr Asp Gly Tyr Ala Ala Ala Gly Val Glu 
    370                 375                 380                 


Ile Lys Val Val Asp Asp Ala Arg Lys Thr Leu Pro Pro Gly Cys Glu 
385                 390                 395                 400 


Gly Glu Glu Ala Ser Arg Gly Pro Asn Val Phe Met Gly Tyr Phe Asp 
                405                 410                 415     


Glu Pro Glu Leu Thr Ala Arg Ala Leu Asp Glu Glu Gly Trp Tyr Tyr 
            420                 425                 430         


Ser Gly Asp Leu Cys Arg Met Asp Glu Ala Gly Tyr Ile Lys Ile Thr 
        435                 440                 445             


Gly Arg Lys Lys Asp Ile Ile Val Arg Gly Gly Glu Asn Ile Ser Ser 
    450                 455                 460                 


Arg Glu Val Glu Asp Ile Leu Leu Gln His Pro Lys Ile His Asp Ala 
465                 470                 475                 480 


Cys Val Val Ala Met Ser Asp Glu Arg Leu Gly Glu Arg Ser Cys Ala 
                485                 490                 495     


Tyr Val Val Leu Lys Ala Pro His His Ser Leu Ser Leu Glu Glu Val 
            500                 505                 510         


Val Ala Phe Phe Ser Arg Lys Arg Val Ala Lys Tyr Lys Tyr Pro Glu 
        515                 520                 525             


His Ile Val Val Ile Glu Lys Leu Pro Arg Thr Thr Ser Gly Lys Ile 
    530                 535                 540                 


Gln Lys Phe Leu Leu Arg Lys Asp Ile Met Arg Arg Leu Thr Gln Asp 
545                 550                 555                 560 


Val Cys Glu Glu Ile Glu 
                565     


