               SEQUENCE LISTING

<110> Technische Universiteit Delft
      Wageningen Universiteit

<120> Single-molecule FRET for protein characterization

<130> P16000122PC00

<150> NL2023825
<151> 2019-09-12

<160> 5

<170> BiSSAP 1.3.6

<210> 1
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tag for experiment 3 - 1

<400> 1
tttttataca tctatttttt tttttttttt ttttttcttc attacttttt tttttttttt     60


<210> 2
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tag for experiment 3 - 2

<400> 2
tttttttttt tttttttttt tttttataca tctattcttc attacttttt tttttttttt     60


<210> 3
<211> 178
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein for experiment 4 - 1

<220> 
<221> BINDING
<222> 141..146
<223> FGE binding site

<400> 3
Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      
Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          
Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              
Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  
Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  
Thr Val Glu Gly Ala Gly Cys Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      
Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         
Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             
Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala Leu Cys Thr Pro 
    130                 135                 140                 
Ser Arg Tyr Gln Asp Pro Val Gln Val Asp Ala Ala Ala Glu Leu Ala 
145                 150                 155                 160 
Leu Val Pro Arg Gly Ser Ser Ala His His His His His His His His 
                165                 170                 175     
His His 
        

<210> 4
<211> 178
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein for experiment 4 - 2

<220> 
<221> BINDING
<222> 141..146
<223> FGE binding site

<400> 4
Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      
Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          
Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              
Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  
Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  
Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      
Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         
Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             
Cys Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala Leu Cys Thr Pro 
    130                 135                 140                 
Ser Arg Tyr Gln Asp Pro Val Gln Val Asp Ala Ala Ala Glu Leu Ala 
145                 150                 155                 160 
Leu Val Pro Arg Gly Ser Ser Ala His His His His His His His His 
                165                 170                 175     
His His 
        

<210> 5
<211> 178
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein for experiment 4 - 3

<220> 
<221> BINDING
<222> 141..146
<223> FGE binding site

<400> 5
Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      
Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          
Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              
Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  
Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  
Thr Val Glu Gly Ala Gly Cys Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      
Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         
Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             
Cys Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala Leu Cys Thr Pro 
    130                 135                 140                 
Ser Arg Tyr Gln Asp Pro Val Gln Val Asp Ala Ala Ala Glu Leu Ala 
145                 150                 155                 160 
Leu Val Pro Arg Gly Ser Ser Ala His His His His His His His His 
                165                 170                 175     
His His 
        

