                         SEQUENCE LISTING

<110>  BLIRT S.A.
 
<120>  A new, non-specific thermolabile nuclease active at low 
       temperature, in wide pH range and high concentration in salts

<130>  4PCT/MM/2020

<140>  PCT/PL2020/000076
<141>  2020-09-14

<150>  P.431144
<151>  2019-09-13

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  846
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence

<400>  1
caggcgctct tctatgaaat atctgctgcc gaccgcagca gcgggtctgc tgctgctggc       60

agcacagcct gcaatggcag catccatgga gcatcaccat catcatcatg gtagcgaaaa      120

tctgtatttt cagtccgcac cgcctagcag ctttagcaaa gcaaaacgtc tggcagttga      180

aatctatcag gatcatccga ccagctttta ttgtggttgt gcaattacct ggcagggtaa      240

aaaaggtctg ccggatctgg caagctgtgg ttatgaagtt cgtaaacaag aaaaacgtgc      300

caaccgtatt gaatgggaac atgttgttcc ggcagaaaat tttggtcgtg catttgttga      360

atggcgtgaa ggtcatccgc agtgtgttaa tagcaaaggt aaagcatata aaggtcgtag      420

ctgtgccacc aaaatgaatc cgacctatcg tagcatgcag gccgatctgc ataatctgac      480

accggcagtt ggtgaagtta atggtgatcg tagcaattat gcatttctgc cgtggaatgg      540

taataacggt gcattttatg gtcagtgcga tatgaaaatc gacttcaaaa atcgtcgtgc      600

agatcctccg gaacagagcc gtggtgcaat tgcccgtacc tatctgtata tgaatcaaga      660

gtataaaatg gccctgagca gccagcagcg tcagctgatg gaagcatgga atcgtcagta      720

tccgattagc acctgggaat gtgaacgtga tcgtcgtatt gcaaatatcc aggataacca      780

taacagcttt gttctgcagg catgtaaaaa agccggttat tgagtcgact ggtagaagag      840

cgctgc                                                                 846


<210>  2
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence

<400>  2

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Ala Ser Met Glu His His His His His His 
            20                  25                  30          


Gly Ser Glu Asn Leu Tyr Phe Gln Ser Ala Pro Pro Ser Ser Phe Ser 
        35                  40                  45              


Lys Ala Lys Arg Leu Ala Val Glu Ile Tyr Gln Asp His Pro Thr Ser 
    50                  55                  60                  


Phe Tyr Cys Gly Cys Ala Ile Thr Trp Gln Gly Lys Lys Gly Leu Pro 
65                  70                  75                  80  


Asp Leu Ala Ser Cys Gly Tyr Glu Val Arg Lys Gln Glu Lys Arg Ala 
                85                  90                  95      


Asn Arg Ile Glu Trp Glu His Val Val Pro Ala Glu Asn Phe Gly Arg 
            100                 105                 110         


Ala Phe Val Glu Trp Arg Glu Gly His Pro Gln Cys Val Asn Ser Lys 
        115                 120                 125             


Gly Lys Ala Tyr Lys Gly Arg Ser Cys Ala Thr Lys Met Asn Pro Thr 
    130                 135                 140                 


Tyr Arg Ser Met Gln Ala Asp Leu His Asn Leu Thr Pro Ala Val Gly 
145                 150                 155                 160 


Glu Val Asn Gly Asp Arg Ser Asn Tyr Ala Phe Leu Pro Trp Asn Gly 
                165                 170                 175     


Asn Asn Gly Ala Phe Tyr Gly Gln Cys Asp Met Lys Ile Asp Phe Lys 
            180                 185                 190         


Asn Arg Arg Ala Asp Pro Pro Glu Gln Ser Arg Gly Ala Ile Ala Arg 
        195                 200                 205             


Thr Tyr Leu Tyr Met Asn Gln Glu Tyr Lys Met Ala Leu Ser Ser Gln 
    210                 215                 220                 


Gln Arg Gln Leu Met Glu Ala Trp Asn Arg Gln Tyr Pro Ile Ser Thr 
225                 230                 235                 240 


Trp Glu Cys Glu Arg Asp Arg Arg Ile Ala Asn Ile Gln Asp Asn His 
                245                 250                 255     


Asn Ser Phe Val Leu Gln Ala Cys Lys Lys Ala Gly Tyr 
            260                 265                 


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence

<400>  3

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence

<400>  4

Ala Ser Met Glu His His His His His His Gly Ser Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Ser 
            


<210>  5
<211>  4824
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence

<400>  5
tctagaaata attttgttta actttttgag accttaagga ggtaaaaaat gaaatatctg       60

ctgccgaccg cagcagcggg tctgctgctg ctggcagcac agcctgcaat ggcagcatcc      120

atggagcatc accatcatca tcatggtagc gaaaatctgt attttcagtc cgcaccgcct      180

agcagcttta gcaaagcaaa acgtctggca gttgaaatct atcaggatca tccgaccagc      240

ttttattgtg gttgtgcaat tacctggcag ggtaaaaaag gtctgccgga tctggcaagc      300

tgtggttatg aagttcgtaa acaagaaaaa cgtgccaacc gtattgaatg ggaacatgtt      360

gttccggcag aaaattttgg tcgtgcattt gttgaatggc gtgaaggtca tccgcagtgt      420

gttaatagca aaggtaaagc atataaaggt cgtagctgtg ccaccaaaat gaatccgacc      480

tatcgtagca tgcaggccga tctgcataat ctgacaccgg cagttggtga agttaatggt      540

gatcgtagca attatgcatt tctgccgtgg aatggtaata acggtgcatt ttatggtcag      600

tgcgatatga aaatcgactt caaaaatcgt cgtgcagatc ctccggaaca gagccgtggt      660

gcaattgccc gtacctatct gtatatgaat caagagtata aaatggccct gagcagccag      720

cagcgtcagc tgatggaagc atggaatcgt cagtatccga ttagcacctg ggaatgtgaa      780

cgtgatcgtc gtattgcaaa tatccaggat aaccataaca gctttgttct gcaggcatgt      840

aaaaaagccg gttattgagt cgactggttg aggtctcacc ccctagcata accccttggg      900

gcctctaaac gggtcttgag gggttttttg cccctgagac gcgtcaatcg agttcgtacc      960

taagggcgac accccctaat tagcccgggc gaaaggccca gtctttcgac tgagcctttc     1020

gttttatttg atgcctggca gttccctact ctcgcatggg gagtccccac actaccatcg     1080

gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg     1140

ccgccaggca aacaaggggt gttatgagcc atattcaggt ataaatgggc tcgcgataat     1200

gttcagaatt ggttaattgg ttgtaacact gacccctatt tgtttatttt tctaaataca     1260

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa     1320

aaggaagaat atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt     1380

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca     1440

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag     1500

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc     1560

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca     1620

gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt     1680

aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct     1740

gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt     1800

aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga     1860

caccacgatg cctgtagcga tggcaacaac gttgcgcaaa ctattaactg gcgaactact     1920

tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc     1980

acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatccg gagccggtga     2040

gcgtggttct cgcggtatca tcgcagcgct ggggccagat ggtaagccct cccgtatcgt     2100

agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga     2160

gataggtgcc tcactgatta agcattggta agcggcgcgc catcgaatgg cgcaaaacct     2220

ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatatgaaac     2280

cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg     2340

tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg     2400

cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc     2460

tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga     2520

ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg     2580

gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga     2640

tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg     2700

ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc     2760

atgaggacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg     2820

cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata     2880

aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca     2940

tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc     3000

tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc     3060

gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagatagc tcatgttata     3120

tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc     3180

gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg ccagtctcac     3240

tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg     3300

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgactca     3360

tgaccaaaat cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt     3420

agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca     3480

aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct     3540

ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta     3600

gccgtagtta gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct     3660

aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc     3720

aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca     3780

gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga     3840

aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg     3900

aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt     3960

cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag     4020

cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt     4080

tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt     4140

tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga     4200

ggaagcggaa ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac     4260

ggatggcctt tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta     4320

agctcgggcc ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa     4380

aacccgcttc ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata     4440

tttaagggcg cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa     4500

aaaagcaacg cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta     4560

aactattcat ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag     4620

aattaagaaa ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta     4680

tacatgtcaa cgataataca aaatataata caaactataa gatgttatca gtatttatta     4740

tcatttagaa taaattttgt gtcgcccttc cgcgaaatta atacgactca ctatagggga     4800

attgtgagcg gataacaatt cccc                                            4824


