
                                SEQUENCE LISTING

<110> Ntana, Fani
      Collinge, David B.
      Hamberger, Bjorn
      Bhat, Wajid Waheed
      Johnson, Sean
      Jensen, Birgit
      Jorgensen, Hans Jorgen Lyngs
      Board of Trustees of Michigan State University
      University of Copenhagen

<120> METHOD FOR PRODUCING THE SESQUITERPENE VIRIDIFLOROL WITH
      A FUNGAL ENZYME
  

<130> 3000.192WO1

<150> US 62/899,391       
<151> 2019-09-12  

<160> 26

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 523
<212> PRT
<213> Serendipita indica

<400> 1
Met Pro Ser Val Ser Pro Ala Thr Ile Arg Leu Pro Asp Ile Leu Gly
 1               5                  10                  15
Ala Met Asp Arg Phe Glu Leu Arg Thr His Pro Asp Glu Arg Glu Val
            20                  25                  30
Thr Arg Ala Ser Asn Glu Trp Phe Asn Ser Tyr Asn Met Met Pro Pro
        35                  40                  45
Ala Leu Phe Glu Lys Phe Val Lys Cys Asp Phe Gly Leu Met Thr Gly
    50                  55                  60
Met Ser Tyr Pro Asp Thr Asp Ala Thr Arg Leu Arg Ile Thr Cys Asp
65                  70                  75                  80
Tyr Met Ser Ile Leu Phe Ala Tyr Asp Asp Leu Met Asp Leu Pro Ser
                85                  90                  95
Ser Asp Leu Met His Asp Lys Ile Ala Ser Asp Lys Ala Ala Lys Ile
            100                 105                 110
Met Met Gly Val Leu Thr His Pro His Lys Phe Arg Pro Tyr Ala Gly
        115                 120                 125
Leu Pro Val Ala Thr Ala Phe His Asp Phe Trp Thr Arg Phe Cys Ala
    130                 135                 140
Thr Ser Thr Pro Lys Met Gln Lys Arg Phe Thr Asp Thr Thr Tyr Glu
145                 150                 155                 160
Tyr Val Met Ala Val Lys Asn Gln Cys Gly Asn Arg Gln Ser Ser Arg
                165                 170                 175
Cys Pro Thr Ile Glu Glu Tyr Val Ala Leu Arg Arg Asp Thr Ser Ala
            180                 185                 190
Ile Lys Val Thr Tyr Ala Cys Ile Glu Tyr Cys Leu Asn Ile Asp Val
        195                 200                 205
Pro Asp Glu Ala Phe Tyr His Pro Ser Val Ala Ala Leu Gln Glu Ala
    210                 215                 220
Gly Asn Asn Ile Leu Ser Trp Ala Asn Asp Val Tyr Ser Phe Asp Asn
225                 230                 235                 240
Glu Gln Ser Ser Gly Asp Cys His Asn Leu Val Ala Ile Val Ala Ile
                245                 250                 255
Asn Lys Asn Ile Thr Val Gln Ala Ala Met Glu Tyr Val Met Gly Met
            260                 265                 270
Ile Asp Ser Ala Ile Glu Arg Phe Phe Glu Glu Cys Ala Asn Val Pro
        275                 280                 285
Ser Phe Gly Pro Glu Val Asp Pro Leu Val Gln Ala Tyr Ile Lys Gly
    290                 295                 300
Val Glu Leu Tyr Leu Ser Gly Ser Val Phe Trp His Leu Glu Ser Glu
305                 310                 315                 320
Arg Tyr Phe Gly Ala Arg Val Gln His Val Lys Asp Thr Leu Met Val
                325                 330                 335
Glu Leu Arg Pro Leu Asp Glu Gly Ala Lys Pro Ala Phe Asp Leu Met
            340                 345                 350
Tyr Lys Leu Pro Ser Asn Leu Thr Pro Glu Val Leu Ser Ala Ala Ala
        355                 360                 365
Val Ser Ala Ala Pro Ala Ala Pro Ala Pro Val Ala Ser Pro Ala Pro
    370                 375                 380
Gln Pro Glu Ile Leu Ser Pro Thr Pro Ile Ser Pro Ile Asn Val Asn
385                 390                 395                 400
Phe Pro Leu Gly Asn Val Ala Cys Pro Pro Pro Ser Tyr Glu Thr Gln
                405                 410                 415
Arg Val Leu Ala Lys Met Val Ala Ala Thr Val Glu Glu Lys Gln Arg
            420                 425                 430
Leu Ala Tyr Ser Gln Pro Ala Glu Gln Tyr Tyr Ser Pro Ala Pro Gln
        435                 440                 445
Tyr Tyr Pro Ser Gln Pro Val Glu Lys Phe Gln Gln Thr Asn Val Leu
    450                 455                 460
Glu Thr Ala Phe Lys Gly Ser Asn Ser Glu Leu Thr Asn Ile Leu Val
465                 470                 475                 480
Ile Ala Ser Val Leu Met Ala Gly Ser Pro Met Ala Leu Val Pro Phe
                485                 490                 495
Val Pro Leu Leu Ala Leu Leu Leu Leu Pro Asn Glu Thr Pro Val Ala
            500                 505                 510
Pro Val Ala Phe Glu His His His His His His
        515                 520

<210> 2
<211> 1569
<212> DNA
<213> Serendipita indica

<400> 2
atgccatctg tctctcctgc caccatccgc ctccctgata tcctcggtgc tatggaccgc      60
tttgagctcc gcactcaccc cgatgagcgc gaagtcaccc gtgcctcgaa cgagtggttc     120
aacagctaca acatgatgcc cccggcactc tttgaaaagt ttgtcaagtg cgatttcggc     180
ctcatgaccg gcatgtcgta cccagatacc gatgctaccc gcctccgtat cacttgcgac     240
tacatgtcga tcctcttcgc ctatgacgac ctcatggacc tcccctcgtc cgaccttatg     300
catgacaaga ttgcctcgga caaggctgcc aagatcatga tgggtgtcct cacccacccc     360
cacaagttcc gcccatatgc tggcctccca gtcgccactg ctttccatga cttctggacc     420
cgcttctgcg ctacttcgac cccaaagatg caaaagcgct tcactgacac cacctacgag     480
tatgtcatgg ccgtcaagaa ccagtgcggc aaccgccaga gctctcgctg cccaaccatc     540
gaggagtacg tcgctctccg ccgcgacacc tcggccatca aggtcaccta tgcttgcatc     600
gagtactgcc tcaacatcga cgtcccagac gaggccttct accacccctc cgtggctgct     660
ctccaggagg ctggcaataa tatcctctcg tgggccaacg atgtttactc gtttgacaac     720
gagcaatcct cgggtgactg ccacaacctc gttgccattg ttgccatcaa caagaacatt     780
actgttcagg ctgcaatgga gtacgtcatg ggcatgatcg actctgctat cgaacgcttc     840
ttcgaggagt gcgccaacgt cccttcgttc ggccccgaag tcgaccctct cgtccaggcc     900
tacatcaagg gtgtcgagct ctaccttagc ggctccgtct tctggcacct cgaatccgag     960
cgctactttg gtgctcgcgt ccagcacgtc aaggatacct tgatggttga gctccgccca    1020
ctcgacgagg gtgcgaagcc ggccttcgac ctcatgtaca agctcccatc caacttgacg    1080
cccgaggtcc tcagtgccgc tgctgtctcg gctgccccag ctgcgccagc tcctgtcgct    1140
tctccggctc ctcagccaga gatcctctcg ccgacgccaa tctcgcccat caacgtcaac    1200
ttccctctcg gcaacgtcgc ctgcccgcct ccttcgtacg agacccagcg cgttctcgcc    1260
aagatggtgg ccgcgaccgt cgaggagaag cagcgccttg cttacagcca gccagctgag    1320
cagtactact cgcccgctcc ccagtactac ccaagccagc cggttgaaaa gttccagcag    1380
accaacgtgc tcgagaccgc cttcaaggga tccaactcgg aattgaccaa cattctcgtt    1440
attgcctccg tcctcatggc cggatcaccc atggcgcttg tcccctttgt ccctcttctc    1500
gccctcctac tcctccccaa cgagacccca gtggctcccg ttgcgttcga gcaccaccac    1560
caccaccac                                                            1569

<210> 3
<211> 517
<212> PRT
<213> Serendipita indica

<400> 3
Met Pro Ser Val Ser Pro Ala Thr Ile Arg Leu Pro Asp Ile Leu Gly
 1               5                  10                  15
Ala Met Asp Arg Phe Glu Leu Arg Thr His Pro Asp Glu Arg Glu Val
            20                  25                  30
Thr Arg Ala Ser Asn Glu Trp Phe Asn Ser Tyr Asn Met Met Pro Pro
        35                  40                  45
Ala Leu Phe Glu Lys Phe Val Lys Cys Asp Phe Gly Leu Met Thr Gly
    50                  55                  60
Met Ser Tyr Pro Asp Thr Asp Ala Thr Arg Leu Arg Ile Thr Cys Asp
65                  70                  75                  80
Tyr Met Ser Ile Leu Phe Ala Tyr Asp Asp Leu Met Asp Leu Pro Ser
                85                  90                  95
Ser Asp Leu Met His Asp Lys Ile Ala Ser Asp Lys Ala Ala Lys Ile
            100                 105                 110
Met Met Gly Val Leu Thr His Pro His Lys Phe Arg Pro Tyr Ala Gly
        115                 120                 125
Leu Pro Val Ala Thr Ala Phe His Asp Phe Trp Thr Arg Phe Cys Ala
    130                 135                 140
Thr Ser Thr Pro Lys Met Gln Lys Arg Phe Thr Asp Thr Thr Tyr Glu
145                 150                 155                 160
Tyr Val Met Ala Val Lys Asn Gln Cys Gly Asn Arg Gln Ser Ser Arg
                165                 170                 175
Cys Pro Thr Ile Glu Glu Tyr Val Ala Leu Arg Arg Asp Thr Ser Ala
            180                 185                 190
Ile Lys Val Thr Tyr Ala Cys Ile Glu Tyr Cys Leu Asn Ile Asp Val
        195                 200                 205
Pro Asp Glu Ala Phe Tyr His Pro Ser Val Ala Ala Leu Gln Glu Ala
    210                 215                 220
Gly Asn Asn Ile Leu Ser Trp Ala Asn Asp Val Tyr Ser Phe Asp Asn
225                 230                 235                 240
Glu Gln Ser Ser Gly Asp Cys His Asn Leu Val Ala Ile Val Ala Ile
                245                 250                 255
Asn Lys Asn Ile Thr Val Gln Ala Ala Met Glu Tyr Val Met Gly Met
            260                 265                 270
Ile Asp Ser Ala Ile Glu Arg Phe Phe Glu Glu Cys Ala Asn Val Pro
        275                 280                 285
Ser Phe Gly Pro Glu Val Asp Pro Leu Val Gln Ala Tyr Ile Lys Gly
    290                 295                 300
Val Glu Leu Tyr Leu Ser Gly Ser Val Phe Trp His Leu Glu Ser Glu
305                 310                 315                 320
Arg Tyr Phe Gly Ala Arg Val Gln His Val Lys Asp Thr Leu Met Val
                325                 330                 335
Glu Leu Arg Pro Leu Asp Glu Gly Ala Lys Pro Ala Phe Asp Leu Met
            340                 345                 350
Tyr Lys Leu Pro Ser Asn Leu Thr Pro Glu Val Leu Ser Ala Ala Ala
        355                 360                 365
Val Ser Ala Ala Pro Ala Ala Pro Ala Pro Val Ala Ser Pro Ala Pro
    370                 375                 380
Gln Pro Glu Ile Leu Ser Pro Thr Pro Ile Ser Pro Ile Asn Val Asn
385                 390                 395                 400
Phe Pro Leu Gly Asn Val Ala Cys Pro Pro Pro Ser Tyr Glu Thr Gln
                405                 410                 415
Arg Val Leu Ala Lys Met Val Ala Ala Thr Val Glu Glu Lys Gln Arg
            420                 425                 430
Leu Ala Tyr Ser Gln Pro Ala Glu Gln Tyr Tyr Ser Pro Ala Pro Gln
        435                 440                 445
Tyr Tyr Pro Ser Gln Pro Val Glu Lys Phe Gln Gln Thr Asn Val Leu
    450                 455                 460
Glu Thr Ala Phe Lys Gly Ser Asn Ser Glu Leu Thr Asn Ile Leu Val
465                 470                 475                 480
Ile Ala Ser Val Leu Met Ala Gly Ser Pro Met Ala Leu Val Pro Phe
                485                 490                 495
Val Pro Leu Leu Ala Leu Leu Leu Leu Pro Asn Glu Thr Pro Val Ala
            500                 505                 510
Pro Val Ala Phe Glu
        515

<210> 4
<211> 522
<212> PRT
<213> Serendipita indica

<400> 4
Met Pro Ser Val Ser Pro Ala Thr Ile Arg Leu Pro Asp Ile Leu Gly
 1               5                  10                  15
Ala Met Asp Arg Phe Glu Leu Arg Thr His Pro Asp Glu Arg Glu Val
            20                  25                  30
Thr Arg Ala Ser Asn Glu Trp Phe Asn Ser Tyr Asn Met Met Pro Pro
        35                  40                  45
Ala Leu Phe Glu Lys Phe Val Lys Cys Asp Phe Gly Leu Met Thr Gly
    50                  55                  60
Met Ser Tyr Pro Asp Thr Asp Ala Thr Arg Leu Arg Ile Thr Cys Asp
65                  70                  75                  80
Tyr Met Ser Ile Leu Phe Ala Tyr Asp Asp Leu Met Asp Leu Pro Ser
                85                  90                  95
Ser Asp Leu Met His Asp Lys Ile Ala Ser Asp Lys Ala Ala Lys Ile
            100                 105                 110
Met Met Gly Val Leu Thr His Pro His Lys Phe Arg Pro Tyr Ala Gly
        115                 120                 125
Leu Pro Val Ala Thr Ala Phe His Asp Phe Trp Thr Arg Phe Cys Ala
    130                 135                 140
Thr Ser Thr Pro Lys Met Gln Lys Arg Phe Thr Asp Thr Thr Tyr Glu
145                 150                 155                 160
Tyr Val Met Ala Val Lys Asn Gln Cys Gly Asn Arg Gln Ser Ser Arg
                165                 170                 175
Cys Pro Thr Ile Glu Glu Tyr Val Ala Leu Arg Arg Asp Thr Ser Ala
            180                 185                 190
Ile Lys Val Thr Tyr Ala Cys Ile Glu Tyr Cys Leu Asn Ile Asp Val
        195                 200                 205
Pro Asp Glu Ala Phe Tyr His Pro Ser Val Ala Ala Leu Gln Glu Ala
    210                 215                 220
Gly Asn Ile Leu Ser Trp Ala Asn Asp Val Tyr Ser Phe Asp Asn Glu
225                 230                 235                 240
Gln Ser Ser Gly Asp Cys His Asn Leu Val Ala Ile Val Ala Ile Asn
                245                 250                 255
Lys Asn Ile Thr Val Gln Ala Ala Met Glu Tyr Val Met Gly Met Ile
            260                 265                 270
Asp Ser Ala Ile Glu Arg Phe Phe Glu Glu Cys Ala Asn Val Pro Ser
        275                 280                 285
Phe Gly Pro Glu Val Asp Pro Leu Val Gln Ala Tyr Ile Lys Gly Val
    290                 295                 300
Glu Leu Tyr Leu Ser Gly Ser Val Phe Trp His Leu Glu Ser Glu Arg
305                 310                 315                 320
Tyr Phe Gly Ala Arg Val Gln His Val Lys Asp Thr Leu Met Val Glu
                325                 330                 335
Leu Arg Pro Leu Asp Glu Gly Ala Lys Pro Ala Phe Asp Leu Met Tyr
            340                 345                 350
Lys Leu Pro Ser Asn Leu Thr Pro Glu Val Leu Ser Ala Ala Ala Val
        355                 360                 365
Ser Ala Ala Pro Ala Ala Pro Ala Pro Val Ala Ser Pro Ala Pro Gln
    370                 375                 380
Pro Glu Ile Leu Ser Pro Thr Pro Ile Ser Pro Ile Asn Val Asn Phe
385                 390                 395                 400
Pro Leu Gly Asn Val Ala Cys Pro Pro Pro Ser Tyr Glu Thr Gln Arg
                405                 410                 415
Val Leu Ala Lys Met Val Ala Ala Thr Val Glu Glu Lys Gln Arg Leu
            420                 425                 430
Ala Tyr Ser Gln Pro Ala Glu Gln Tyr Tyr Ser Pro Ala Pro Gln Tyr
        435                 440                 445
Tyr Pro Ser Gln Pro Val Glu Lys Phe Gln Gln Thr Asn Val Leu Glu
    450                 455                 460
Thr Ala Phe Lys Gly Ser Asn Ser Glu Leu Thr Asn Ile Leu Val Ile
465                 470                 475                 480
Ala Ser Val Leu Met Ala Gly Ser Pro Met Ala Leu Val Pro Phe Val
                485                 490                 495
Pro Leu Leu Ala Leu Leu Leu Leu Pro Asn Glu Thr Pro Val Ala Pro
            500                 505                 510
Val Ala Phe Glu His His His His His His
        515                 520

<210> 5
<211> 295
<212> PRT
<213> Cladonia uncialis

<400> 5
Met Cys Ala Glu Trp Ile Thr Val Leu Phe Val Trp Asp Asp Leu Leu
 1               5                  10                  15
Asp Val Pro Ile Asp Ser Asp Leu Val Ser Asp Glu Gln Gly Thr Arg
            20                  25                  30
Glu Ile Asn Arg Val Met Ser Cys Ile Leu Thr Gln Pro Glu Asn Phe
        35                  40                  45
Glu Pro Met Val Thr Gln Pro Val Thr Gly Ala Leu His Ser Phe Trp
    50                  55                  60
Thr Gln Phe Cys Ala Thr Ser Ser Pro Asn Met Gln Lys Arg Phe Val
65                  70                  75                  80
Glu Ala Val Leu Lys Tyr Ala Glu Gly Ala Ala Lys Gln Val Ala Ser
                85                  90                  95
Arg Glu Thr Arg Ala Leu Pro Ser Ile Lys Asp Phe Ile Val Asn Arg
            100                 105                 110
Gln Ser Ala Ser Gly Val Glu Thr Leu Leu Ala Leu Val Glu Tyr Cys
        115                 120                 125
Leu Gln Ile Gln Val Pro Asp Cys Ala Tyr Tyr His Pro Thr Leu Gln
    130                 135                 140
Gln Leu Arg Asn Ser Ile Asn Glu Ile Val Ser Trp Ser Asn Asp Ile
145                 150                 155                 160
Tyr Ser Phe Asn Lys Glu Gln Ala Cys Gly Asp His Ala Asn Leu Val
                165                 170                 175
Val Val Val Ala Ile Glu Lys Gly Ile Pro Val Gln Ser Ala Ile Thr
            180                 185                 190
Tyr Val Ser Val Met Val Gln Glu Ala Val Lys Arg Tyr His Glu Asn
        195                 200                 205
Leu Lys Lys Ile Pro Lys Phe Asp Pro Arg Ile Asp Ala Leu Val Leu
    210                 215                 220
Lys Tyr Val Gly Gly Ile Glu Cys Val Cys Thr Gly Leu Val Ser Trp
225                 230                 235                 240
His Phe Lys Ile Asp Arg Tyr Phe Gly Glu Asn Ser Ser Glu Val Ser
                245                 250                 255
Asn Thr Leu Met Val Asp Leu Leu Pro Gln Glu Lys Asn Ala Leu Val
            260                 265                 270
Ser Ala His Glu Leu Gln Tyr Asp Gln Leu Pro Ile Ser Thr Pro Gln
        275                 280                 285
Pro Lys Gly Ser Ala Met Thr
    290                 295

<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> A synthetic polypeptide

<400> 6
His His His His His His
 1               5

<210> 7

<400> 7
 000

<210> 8

<400> 8
 000

<210> 9

<400> 9
 000

<210> 10

<400> 10
 000

<210> 11
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 11
agaaggagat ataccatgcc atctgtctct cctgccac                              38

<210> 12
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 12
ggtggtggtg ctcgaacgca acgggagcca ctggg                                 35

<210> 13
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 13
ctccaaaaac agtcgatgcc atctgtctct cctgc                                 35

<210> 14
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 14
tagatatcgt agtttgcaac gggagccact ggggt                                 35

<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 15
cgatacctac ccgcctacaa                                                  20

<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 16
ctttttaagc ggtgctggag                                                  20

<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 17
atgagtacga ttgcccaagg                                                  20

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 18
tcgtctcgta ggcgactttt                                                  20

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 19
agatatccgg aggcgagttt                                                  20

<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 20
cctgaatctg ctgttcgtca                                                  20

<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 21
acatctggct cccatttacg                                                  20

<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 22
gttgagcttt ggctcgtttc                                                  20

<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 23
caacacatgt gcacgtcgat                                                  20

<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 24
ccaatgtgca ttcagaacga                                                  20

<210> 25
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 25
aacctccatt caggagatgt tt                                               22

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic oligonucleotide

<400> 26
tgctgtagca tcctggtatt                                                  20
