                               SEQUENCE LISTING

<110> BROWN UNIVERSITY
 
<120> METHODS AND COMPOSITIONS FOR TREATING, PREVENTING OR REVERSING 
      OBESITY AND OBESITY-RELATED DISORDERS BY OPSIN 3 REGULATION OF 
      HYPOTHALAMIC MELANOCORTIN RECEPTORS

<130> 405505-606001WO

<140> PCT/US2020/052676
<141> 2020-09-25

<150> 62/905,765
<151> 2019-09-25

<160> 12    

<170> PatentIn version 3.5

<210> 1
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
ccggcgcatc taaggtcgat gtcatctcga gatgacatcg accttagatg cgttttt          57


<210> 2
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
ccggcattac ctatatctgg ctctactcga gtagagccag atataggtaa tgttttt          57


<210> 3
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
ccggcgactg agcaagacaa attatctcga gataatttgt cttgctcagt cgttttt          57


<210> 4
<211> 202
<212> PRT
<213> Homo sapiens

<400> 4
Met Ala Val Gln Gly Ser Gln Arg Arg Leu Leu Gly Ser Leu Asn Ser 
1               5                   10                  15      


Thr Pro Thr Ala Ile Pro Gln Leu Gly Leu Ala Ala Asn Gln Thr Gly 
            20                  25                  30          


Ala Arg Cys Leu Glu Val Ser Ile Ser Asp Gly Leu Phe Leu Ser Leu 
        35                  40                  45              


Gly Leu Val Ser Leu Val Glu Asn Ala Leu Val Val Ala Thr Ile Ala 
    50                  55                  60                  


Lys Asn Arg Asn Leu His Ser Pro Met Tyr Cys Phe Ile Cys Cys Leu 
65                  70                  75                  80  


Ala Leu Ser Asp Leu Leu Val Ser Gly Ser Asn Met Leu Glu Thr Ala 
                85                  90                  95      


Val Ile Leu Leu Leu Glu Ala Gly Ala Leu Val Ala Arg Ala Ala Val 
            100                 105                 110         


Leu Gln Gln Leu Asp Asn Val Ile Asp Val Ile Thr Cys Ser Ser Met 
        115                 120                 125             


Leu Ser Ser Leu Cys Phe Leu Gly Ala Ile Ala Val Asp Arg Tyr Ile 
    130                 135                 140                 


Ser Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile Val Thr Leu Pro Arg 
145                 150                 155                 160 


Ala Arg Arg Ala Val Ala Ala Ile Trp Val Ala Ser Val Val Phe Ser 
                165                 170                 175     


Thr Leu Phe Ile Ala Tyr Tyr Asp His Val Ala Val Leu Leu Cys Leu 
            180                 185                 190         


Val Val Phe Phe Leu Ala Met Leu Val Leu 
        195                 200         


<210> 5
<211> 85
<212> PRT
<213> Homo sapiens

<400> 5
Ala Val Leu Tyr Val His Met Leu Ala Arg Ala Cys Gln His Ala Gln 
1               5                   10                  15      


Gly Ile Ala Arg Leu His Lys Arg Gln Arg Pro Val His Gln Gly Phe 
            20                  25                  30          


Gly Leu Lys Gly Ala Val Thr Leu Thr Ile Leu Leu Gly Ile Phe Phe 
        35                  40                  45              


Leu Cys Trp Gly Pro Phe Phe Leu His Leu Thr Leu Ile Val Leu Cys 
    50                  55                  60                  


Pro Glu His Pro Thr Cys Gly Cys Ile Phe Lys Asn Phe Asn Leu Phe 
65                  70                  75                  80  


Leu Ala Leu Ile Ile 
                85  


<210> 6
<211> 28
<212> PRT
<213> Homo sapiens

<400> 6
Asn Ala Ile Ile Asp Pro Leu Ile Tyr Ala Phe His Ser Gln Glu Leu 
1               5                   10                  15      


Arg Arg Thr Leu Lys Glu Val Leu Thr Cys Ser Trp 
            20                  25              


<210> 7
<211> 297
<212> PRT
<213> Homo sapiens

<400> 7
Met Lys His Ile Ile Asn Ser Tyr Glu Asn Ile Asn Asn Thr Ala Arg 
1               5                   10                  15      


Asn Asn Ser Asp Cys Pro Arg Val Val Leu Pro Glu Glu Ile Phe Phe 
            20                  25                  30          


Thr Ile Ser Ile Val Gly Val Leu Glu Asn Leu Ile Val Leu Leu Ala 
        35                  40                  45              


Val Phe Lys Asn Lys Asn Leu Gln Ala Pro Met Tyr Phe Phe Ile Cys 
    50                  55                  60                  


Ser Leu Ala Ile Ser Asp Met Leu Gly Ser Leu Tyr Lys Ile Leu Glu 
65                  70                  75                  80  


Asn Ile Leu Ile Ile Leu Arg Asn Met Gly Tyr Leu Lys Pro Arg Gly 
                85                  90                  95      


Ser Phe Glu Thr Thr Ala Asp Asp Ile Ile Asp Ser Leu Phe Val Leu 
            100                 105                 110         


Ser Leu Leu Gly Ser Ile Phe Ser Leu Ser Val Ile Ala Ala Asp Arg 
        115                 120                 125             


Tyr Ile Thr Ile Phe His Ala Leu Arg Tyr His Ser Ile Val Thr Met 
    130                 135                 140                 


Arg Arg Thr Val Val Val Leu Thr Val Ile Trp Thr Phe Cys Thr Gly 
145                 150                 155                 160 


Thr Gly Ile Thr Met Val Ile Phe Ser His His Val Pro Thr Val Ile 
                165                 170                 175     


Thr Phe Thr Ser Leu Phe Pro Leu Met Leu Val Phe Ile Leu Cys Leu 
            180                 185                 190         


Tyr Val His Met Phe Leu Leu Ala Arg Ser His Thr Arg Lys Ile Ser 
        195                 200                 205             


Thr Leu Pro Arg Ala Asn Met Lys Gly Ala Ile Thr Leu Thr Ile Leu 
    210                 215                 220                 


Leu Gly Val Phe Ile Phe Cys Trp Ala Pro Phe Val Leu His Val Leu 
225                 230                 235                 240 


Leu Met Thr Phe Cys Pro Ser Asn Pro Tyr Cys Ala Cys Tyr Met Ser 
                245                 250                 255     


Leu Phe Gln Val Asn Gly Met Leu Ile Met Cys Asn Ala Val Ile Asp 
            260                 265                 270         


Pro Phe Ile Tyr Ala Phe Arg Ser Pro Glu Leu Arg Asp Ala Phe Lys 
        275                 280                 285             


Lys Met Ile Phe Cys Ser Arg Tyr Trp 
    290                 295         


<210> 8
<211> 239
<212> PRT
<213> Homo sapiens

<400> 8
Met Ser Ile Gln Lys Thr Tyr Leu Glu Gly Asp Phe Val Phe Pro Val 
1               5                   10                  15      


Ser Ser Ser Ser Phe Leu Arg Thr Leu Leu Glu Pro Gln Leu Gly Ser 
            20                  25                  30          


Ala Leu Leu Thr Ala Met Asn Ala Ser Cys Cys Leu Pro Ser Val Gln 
        35                  40                  45              


Pro Thr Leu Pro Asn Gly Ser Glu His Leu Gln Ala Pro Phe Phe Ser 
    50                  55                  60                  


Asn Gln Ser Ser Ser Ala Phe Cys Glu Gln Val Phe Ile Lys Pro Glu 
65                  70                  75                  80  


Val Phe Leu Ser Leu Gly Ile Val Ser Leu Leu Glu Asn Ile Leu Val 
                85                  90                  95      


Ile Leu Ala Val Val Arg Asn Gly Asn Leu His Ser Pro Met Tyr Phe 
            100                 105                 110         


Phe Leu Cys Ser Leu Ala Val Ala Asp Met Leu Val Ser Val Ser Asn 
        115                 120                 125             


Ala Leu Glu Thr Ile Met Ile Ala Ile Val His Ser Asp Tyr Leu Thr 
    130                 135                 140                 


Phe Glu Asp Gln Phe Ile Gln His Met Asp Asn Ile Phe Asp Ser Met 
145                 150                 155                 160 


Ile Cys Ile Ser Leu Val Ala Ser Ile Cys Asn Leu Leu Ala Ile Ala 
                165                 170                 175     


Val Asp Arg Tyr Val Thr Ile Phe Tyr Ala Leu Arg Tyr His Ser Ile 
            180                 185                 190         


Met Thr Val Arg Lys Ala Leu Thr Leu Ile Val Ala Ile Trp Val Cys 
        195                 200                 205             


Cys Gly Val Cys Gly Val Val Phe Ile Val Tyr Ser Glu Ser Lys Met 
    210                 215                 220                 


Val Ile Val Cys Leu Ile Thr Met Phe Phe Ala Met Met Leu Leu 
225                 230                 235                 


<210> 9
<211> 86
<212> PRT
<213> Homo sapiens

<400> 9
Gly Thr Leu Tyr Val His Met Phe Leu Phe Ala Arg Leu His Val Lys 
1               5                   10                  15      


Arg Ile Ala Ala Leu Pro Pro Ala Asp Gly Val Ala Pro Gln Gln His 
            20                  25                  30          


Ser Cys Met Lys Gly Ala Val Thr Ile Thr Ile Leu Leu Gly Val Phe 
        35                  40                  45              


Ile Phe Cys Trp Ala Pro Phe Phe Leu His Leu Val Leu Ile Ile Thr 
    50                  55                  60                  


Cys Pro Thr Asn Pro Tyr Cys Ile Cys Tyr Thr Ala His Phe Asn Thr 
65                  70                  75                  80  


Tyr Leu Val Leu Ile Met 
                85      


<210> 10
<211> 33
<212> PRT
<213> Homo sapiens

<400> 10
Asn Ser Val Ile Asp Pro Leu Ile Tyr Ala Phe Arg Ser Leu Glu Leu 
1               5                   10                  15      


Arg Asn Thr Phe Arg Glu Ile Leu Cys Gly Cys Asn Gly Met Asn Leu 
            20                  25                  30          


Gly 
    


<210> 11
<211> 332
<212> PRT
<213> Homo sapiens

<400> 11
Met Val Asn Ser Thr His Arg Gly Met His Thr Ser Leu His Leu Trp 
1               5                   10                  15      


Asn Arg Ser Ser Tyr Arg Leu His Ser Asn Ala Ser Glu Ser Leu Gly 
            20                  25                  30          


Lys Gly Tyr Ser Asp Gly Gly Cys Tyr Glu Gln Leu Phe Val Ser Pro 
        35                  40                  45              


Glu Val Phe Val Thr Leu Gly Val Ile Ser Leu Leu Glu Asn Ile Leu 
    50                  55                  60                  


Val Ile Val Ala Ile Ala Lys Asn Lys Asn Leu His Ser Pro Met Tyr 
65                  70                  75                  80  


Phe Phe Ile Cys Ser Leu Ala Val Ala Asp Met Leu Val Ser Val Ser 
                85                  90                  95      


Asn Gly Ser Glu Thr Ile Val Ile Thr Leu Leu Asn Ser Thr Asp Thr 
            100                 105                 110         


Asp Ala Gln Ser Phe Thr Val Asn Ile Asp Asn Val Ile Asp Ser Val 
        115                 120                 125             


Ile Cys Ser Ser Leu Leu Ala Ser Ile Cys Ser Leu Leu Ser Ile Ala 
    130                 135                 140                 


Val Asp Arg Tyr Phe Thr Ile Phe Tyr Ala Leu Gln Tyr His Asn Ile 
145                 150                 155                 160 


Met Thr Val Lys Arg Val Gly Ile Ile Ile Ser Cys Ile Trp Ala Ala 
                165                 170                 175     


Cys Thr Val Ser Gly Ile Leu Phe Ile Ile Tyr Ser Asp Ser Ser Ala 
            180                 185                 190         


Val Ile Ile Cys Leu Ile Thr Met Phe Phe Thr Met Leu Ala Leu Met 
        195                 200                 205             


Ala Ser Leu Tyr Val His Met Phe Leu Met Ala Arg Leu His Ile Lys 
    210                 215                 220                 


Arg Ile Ala Val Leu Pro Gly Thr Gly Ala Ile Arg Gln Gly Ala Asn 
225                 230                 235                 240 


Met Lys Gly Ala Ile Thr Leu Thr Ile Leu Ile Gly Val Phe Val Val 
                245                 250                 255     


Cys Trp Ala Pro Phe Phe Leu His Leu Ile Phe Tyr Ile Ser Cys Pro 
            260                 265                 270         


Gln Asn Pro Tyr Cys Val Cys Phe Met Ser His Phe Asn Leu Tyr Leu 
        275                 280                 285             


Ile Leu Ile Met Cys Asn Ser Ile Ile Asp Pro Leu Ile Tyr Ala Leu 
    290                 295                 300                 


Arg Ser Gln Glu Leu Arg Lys Thr Phe Lys Glu Ile Ile Cys Cys Tyr 
305                 310                 315                 320 


Pro Leu Gly Gly Leu Cys Asp Leu Ser Ser Arg Tyr 
                325                 330         


<210> 12
<211> 325
<212> PRT
<213> Homo sapiens

<400> 12
Met Asn Ser Ser Phe His Leu His Phe Leu Asp Leu Asn Leu Asn Ala 
1               5                   10                  15      


Thr Glu Gly Asn Leu Ser Gly Pro Asn Val Lys Asn Lys Ser Ser Pro 
            20                  25                  30          


Cys Glu Asp Met Gly Ile Ala Val Glu Val Phe Leu Thr Leu Gly Val 
        35                  40                  45              


Ile Ser Leu Leu Glu Asn Ile Leu Val Ile Gly Ala Ile Val Lys Asn 
    50                  55                  60                  


Lys Asn Leu His Ser Pro Met Tyr Phe Phe Val Cys Ser Leu Ala Val 
65                  70                  75                  80  


Ala Asp Met Leu Val Ser Met Ser Ser Ala Trp Glu Thr Ile Thr Ile 
                85                  90                  95      


Tyr Leu Leu Asn Asn Lys His Leu Val Ile Ala Asp Ala Phe Val Arg 
            100                 105                 110         


His Ile Asp Asn Val Phe Asp Ser Met Ile Cys Ile Ser Val Val Ala 
        115                 120                 125             


Ser Met Cys Ser Leu Leu Ala Ile Ala Val Asp Arg Tyr Val Thr Ile 
    130                 135                 140                 


Phe Tyr Ala Leu Arg Tyr His His Ile Met Thr Ala Arg Arg Ser Gly 
145                 150                 155                 160 


Ala Ile Ile Ala Gly Ile Trp Ala Phe Cys Thr Gly Cys Gly Ile Val 
                165                 170                 175     


Phe Ile Leu Tyr Ser Glu Ser Thr Tyr Val Ile Leu Cys Leu Ile Ser 
            180                 185                 190         


Met Phe Phe Ala Met Leu Phe Leu Leu Val Ser Leu Tyr Ile His Met 
        195                 200                 205             


Phe Leu Leu Ala Arg Thr His Val Lys Arg Ile Ala Ala Leu Pro Gly 
    210                 215                 220                 


Ala Ser Ser Ala Arg Gln Arg Thr Ser Met Gln Gly Ala Val Thr Val 
225                 230                 235                 240 


Thr Met Leu Leu Gly Val Phe Thr Val Cys Trp Ala Pro Phe Phe Leu 
                245                 250                 255     


His Leu Thr Leu Met Leu Ser Cys Pro Gln Asn Leu Tyr Cys Ser Arg 
            260                 265                 270         


Phe Met Ser His Phe Asn Met Tyr Leu Ile Leu Ile Met Cys Asn Ser 
        275                 280                 285             


Val Met Asp Pro Leu Ile Tyr Ala Phe Arg Ser Gln Glu Met Arg Lys 
    290                 295                 300                 


Thr Phe Lys Glu Ile Ile Cys Cys Arg Gly Phe Arg Ile Ala Cys Ser 
305                 310                 315                 320 


Phe Pro Arg Arg Asp 
                325 


