                         SEQUENCE LISTING

<110>  The University of Manchester
 
<120>  MODULATING OPSIN SIGNALING LIFETIME FOR OPTOGENETIC APPLICATIONS

<130>  P263175WO

<150>  GB 1914826.1
<151>  2019-10-14

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  1218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Arrestin 3A mutant

<400>  1
atggcagcca gcgggaagac cagcaagtcc gaaccgaacc atgttatctt caagaagatc       60

tcccgggaca aatcggtgac catctacctg gggaacagag actacataga ccatgtcagc      120

caagtccagc ctgtggatgg tgtcgtgttg gttgatcctg atcttgtgaa gggaaagaaa      180

gtgtatgtca ctctgacctg cgccttccgc tatggccaag aggacattga cgtgatcggc      240

ttgaccttcc gcagggacct gtacttctcc cgggtccagg tgtatcctcc tgtgggggcc      300

gcgagcaccc ccacaaaact gcaagagagc ctgcttaaaa agctggggag caacacgtac      360

ccctttctcc tgacgtttcc tgactacttg ccctgttcag tgatgttgca gccagctcca      420

caagattcag ggaagtcctg tggggttgac tttgaggtca aagcattcgc cacagacagc      480

accgatgccg aagaggacaa aatccccaag aagagctccg tgcgattact gatccgcaaa      540

gtacagcatg ccccacttga gatgggtccc cagccccgag ctgaggcggc ctggcagttc      600

ttcatgtctg acaagcccct gcaccttgcg gtctctctca acaaagagat ctatttccat      660

ggggagccca tccctgtgac cgtgactgtc accaataaca cagagaagac cgtgaagaag      720

attaaagcat tcgtggaaca ggtggccaat gtggttctct actcgagtga ttattacgtc      780

aagcccgtgg ctatggagga agcgcaagaa aaagtgccac caaacagcac tttgaccaag      840

acgctgacgc tgctgccctt gctggctaac aatcgagaaa ggagaggcat tgccctggat      900

gggaaaatca agcacgagga cacaaacctt gcctccagca ccatcattaa ggagggcata      960

gaccggaccg tcctgggaat cctggtgtct taccagatca aggtgaagct cacagtgtca     1020

ggctttctgg gagagctcac ctccagtgaa gtcgccactg aggtcccatt ccgcctcatg     1080

caccctcagc ctgaggaccc agctaaggaa agttatcagg atgcaaatgc agctgctgag     1140

gagtttgctc gccataatct gaaagatgca ggagaagctg aggaggggaa gagagacaag     1200

aatgacgttg atgagtaa                                                   1218


<210>  2
<211>  1218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Arrestin KEQ3A mutant

<400>  2
atggcagcca gcgggaagac cagcaagtcc gaaccgaacc atgttatctt caagaagatc       60

tcccgggaca aatcggtgac catctacctg gggaacagag actacataga ccatgtcagc      120

caagtccagc ctgtggatgg tgtcgtgttg gttgatcctg atcttgtgaa gggaaagaaa      180

gtgtatgtca ctctgacctg cgccttccgc tatggccaag aggacattga cgtgatcggc      240

ttgaccttcc gcagggacct gtacttctcc cgggtccagg tgtatcctcc tgtgggggcc      300

gcgagcaccc ccacaaaact gcaagagagc ctgcttaaaa agctggggag caacacgtac      360

ccctttctcc tgacgtttcc tgactacttg ccctgttcag tgatgttgca gccagctcca      420

caagattcag ggaagtcctg tggggttgac tttgaggtca aagcattcgc cacagacagc      480

accgatgccg aagaggacaa aatccccaag aagagctccg tgcgattact gatccgcaaa      540

gtacagcatg ccccacttga gatgggtccc cagccccgag ctgaggcggc ctggcagttc      600

ttcatgtctg acaagcccct gcaccttgcg gtctctctca acaaagagat ctatttccat      660

ggggagccca tccctgtgac cgtgactgtc accaataaca cagagaagac cgtgaagaag      720

attaaagcat tcgtggaaca ggtggccaat gtggttctct actcgagtga ttattacgtc      780

cagcccgtgg ctatggagga agcgcaagaa aaagtgccac caaacagcac tttgaccaag      840

acgctgacgc tgctgccctt gctggctaac aatcgagaaa ggagaggcat tgccctggat      900

gggaaaatca agcacgagga cacaaacctt gcctccagca ccatcattaa ggagggcata      960

gaccggaccg tcctgggaat cctggtgtct tacaagatca aggtgaagct cacagtgtca     1020

ggctttctgg gagagctcac ctccagtcac gtcgccactg aggtcccatt ccgcctcatg     1080

caccctcagc ctgaggaccc agctaaggaa agttatcagg atgcaaatgc agctgctgag     1140

gagtttgctc gccataatct gaaagatgca ggagaagctg aggaggggaa gagagacaag     1200

aatgacgttg atgagtaa                                                   1218


<210>  3
<211>  1692
<212>  DNA
<213>  Homo sapiens

<400>  3
atggatttcg ggtctttgga gaccgtggtg gccaactctg ccttcatcgc cgcccgaggc       60

agctttgacg gcagcagctc ccaaccctcc cgggacaaga agtacctggc caagctcaag      120

ctgcccccgc tgtccaagtg tgagtccctc cgcgacagcc tcagcctgga gtttgagagt      180

gtgtgcttgg agcagcccat cggcaagaag ctctttcagc agttcctaca atcggcagag      240

aagcacctgc cggccctgga gctctggaaa gacatcgagg actatgacac ggcagacaat      300

gacctccagc cacagaaggc ccagaccatc ctggcccagt acctggaccc ccaggccaaa      360

ctcttctgca gcttcctgga tgaggggata gtggcgaagt ttaaggaggg gcctgtggag      420

atccaggacg ggctcttcca gcccctgctg caggccaccc tggcacacct gggccaagcc      480

cccttccagg agtacctggg cagcctgtac ttcctgaggt tcctgcagtg gaagtggctg      540

gaagcccagc ccatggggga ggactggttc ctggacttca gggtcctggg gaaagggggc      600

ttcggggagg tgtcggcctg ccagatgaag gcgaccggca agctgtatgc ctgcaagaag      660

ctgaacaaga agcggctgaa gaagaggaag ggctaccagg gtgctatggt ggagaagaag      720

attctgatga aagtacacag caggttcatc gtgtctctgg cctatgcgtt tgaaaccaaa      780

gccgacctct gtctggtgat gaccatcatg aacggaggtg acatcaggta ccacatctac      840

aacgtgaatg aggagaaccc tggcttcccg gagccgcgcg ccctcttcta cacggcgcag      900

atcatctgcg gcctggagca cctgcaccag aggcggatcg tctaccgcga cctcaagccc      960

gagaacgtgc tgctggacaa tgacggcaat gtccggatct ctgaccttgg gctggccgtg     1020

gagctgctgg acggacagag caagaccaag ggctacgcag ggaccccagg tttcatggcc     1080

cccgagctcc tgcagggcga ggagtacgac ttctccgtgg actactttgc cctgggggtc     1140

accctgtatg agatgattgc ggccagagga cccttccgag cccgtggaga gaaggtggag     1200

aacaaggagc tgaagcaccg gatcatctca gagcccgtga agtaccctga taagttcagc     1260

caggccagca aggacttctg cgaggcgctg ctggagaagg acccggagaa gcgcctgggg     1320

ttcagagatg agacctgcga caagctccgt gcccaccccc tcttcaagga ccttaactgg     1380

aggcagctgg aggctgggat gctgatgccc cctttcatcc cagactccaa aactgtctac     1440

gcaaaggata ttcaggacgt gggtgccttt tccaccgtca aaggtgtggc ctttgacaaa     1500

acagacacag aattctttca ggaatttgcc actggcaact gccccatccc ctggcaggag     1560

gagatgatcg agacgggcat ctttggcgag ctgaacgtgt ggcgctcgga cggtcagatg     1620

ccggacgaca tgaagggcat ctccgggggc tccagctcct cgtccaagtc agggatgtgt     1680

ctggtttcct aa                                                         1692


<210>  4
<211>  1047
<212>  DNA
<213>  Homo sapiens

<400>  4
atgaatggca cagaaggccc taacttctac gtgcccttct ccaatgcgac gggtgtggta       60

cgcagcccct tcgagtaccc acagtactac ctggctgagc catggcagtt ctccatgctg      120

gccgcctaca tgtttctgct gatcgtccta ggcttcccca taaatttcct cacgctctac      180

gtcaccgtcc agcacaagaa gctgcgcacg cctctcaact acatcctgct caacctagcc      240

gtggcagatc tcttcatggt tctaggtggc ttcaccagca ccctctacac ctctctgcat      300

ggatacttcg tcttcgggcc cacaggatgc aatttggagg gcttctttgc caccctgggc      360

ggtgaaattg ccctgtggtc cttggtggtc ctggccatcg agcggtacgt ggtggtgtgt      420

aagcccatga gcaacttccg cttcggggag aaccatgcca tcatgggcgt tgccttcacg      480

tgggtcatgg cgctggcctg cgccgcaccc ccactcgccg gctggtccag gtacatcccc      540

gagggcctgc agtgctcgtg tggaatcgac tactacacgc tcaagccgga ggtcaacaac      600

gagtcttttg tcatctacat gttcgtggtc cacttcacca tccccatgat tatcatcttt      660

ttctgctatg ggcagctcgt cttcaccgtc aaggaggccg ctgcccagca gcaggagtca      720

gccaccacac agaaggcaga gaaggaggtc acccgcatgg tcatcatcat ggtcatcgct      780

ttcctcatat gctgggtgcc ctacgccagc gtggcattct acatcttcac ccaccagggc      840

tccaacttcg gtcccatctt catgaccatc ccagcgttct ttgccaagag cgctgccatc      900

tacaaccctg tcatctatat catgatgaac aagcagttcc ggaactgtat gctgaccacc      960

atctgctgcg gcaagaaccc tctgggagat gatgaagccg ctgccgccgt ggctaaagct     1020

gaagctgctc aagtggcccc tgcctaa                                         1047


<210>  5
<211>  1047
<212>  DNA
<213>  Homo sapiens

<400>  5
atgaatggca cagaaggccc taacttctac gtgcccttct ccaatgcgac gggtgtggta       60

cgcagcccct tcgagtaccc acagtactac ctggctgagc catggcagtt ctccatgctg      120

gccgcctaca tgtttctgct gatcgtccta ggcttcccca taaatttcct cacgctctac      180

gtcaccgtcc agcacaagaa gctgcgcacg cctctcaact acatcctgct caacctagcc      240

gtggcagatc tcttcatggt tctaggtggc ttcaccagca ccctctacac ctctctgcat      300

ggatacttcg tcttcgggcc cacaggatgc aatttggagg gcttctttgc caccctgggc      360

ggtgaaattg ccctgtggtc cttggtggtc ctggccatcg agcggtacgt ggtggtgtgt      420

aagcccatga gcaacttccg cttcggggag aaccatgcca tcatgggcgt tgccttcacg      480

tgggtcatgg cgctggcctg cgccgcaccc ccactcgccg gctggtccag gtacatcccc      540

gagggcctgc agtgctcgtg tggaatcgac tactacacgc tcaagccgga ggtcaacaac      600

gagtcttttg tcatctacat gttcgtggtc cacttcacca tccccatgat tatcatcttt      660

ttctgctatg ggcagctcgt cttcaccgtc aaggaggccg ctgcccagca gcaggagtca      720

gccaccacac agaaggcaga gaaggaggtc acccgcatgg tcatcatcat ggtcatcgct      780

ttcctcatat gctgggtgcc ctacgccagc gtggcattct acatcttcac ccaccagggc      840

tccaacttcg gtcccatctt catgaccatc ccagcgttct ttgccaagag cgctgccatc      900

tacaaccctg tcatctatat catgatgaac aagcagttcc ggaactgcat gctcaccacc      960

atctgctgcg gcaagaaccc actgggtgac gatgaggcct ctgctaccgt gtccaagacg     1020

gagacgagcc aggtggcccc ggcctaa                                         1047


<210>  6
<211>  1218
<212>  DNA
<213>  Homo sapiens

<400>  6
atggcagcca gcgggaagac cagcaagtcc gaaccgaacc atgttatctt caagaagatc       60

tcccgggaca aatcggtgac catctacctg gggaacagag actacataga ccatgtcagc      120

caagtccagc ctgtggatgg tgtcgtgttg gttgatcctg atcttgtgaa gggaaagaaa      180

gtgtatgtca ctctgacctg cgccttccgc tatggccaag aggacattga cgtgatcggc      240

ttgaccttcc gcagggacct gtacttctcc cgggtccagg tgtatcctcc tgtgggggcc      300

gcgagcaccc ccacaaaact gcaagagagc ctgcttaaaa agctggggag caacacgtac      360

ccctttctcc tgacgtttcc tgactacttg ccctgttcag tgatgttgca gccagctcca      420

caagattcag ggaagtcctg tggggttgac tttgaggtca aagcattcgc cacagacagc      480

accgatgccg aagaggacaa aatccccaag aagagctccg tgcgattact gatccgcaaa      540

gtacagcatg ccccacttga gatgggtccc cagccccgag ctgaggcggc ctggcagttc      600

ttcatgtctg acaagcccct gcaccttgcg gtctctctca acaaagagat ctatttccat      660

ggggagccca tccctgtgac cgtgactgtc accaataaca cagagaagac cgtgaagaag      720

attaaagcat tcgtggaaca ggtggccaat gtggttctct actcgagtga ttattacgtc      780

aagcccgtgg ctatggagga agcgcaagaa aaagtgccac caaacagcac tttgaccaag      840

acgctgacgc tgctgccctt gctggctaac aatcgagaaa ggagaggcat tgccctggat      900

gggaaaatca agcacgagga cacaaacctt gcctccagca ccatcattaa ggagggcata      960

gaccggaccg tcctgggaat cctggtgtct taccagatca aggtgaagct cacagtgtca     1020

ggctttctgg gagagctcac ctccagtgaa gtcgccactg aggtcccatt ccgcctcatg     1080

caccctcagc ctgaggaccc agctaaggaa agttatcagg atgcaaattt agtttttgag     1140

gagtttgctc gccataatct gaaagatgca ggagaagctg aggaggggaa gagagacaag     1200

aatgacgttg atgagtaa                                                   1218


<210>  7
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer of Arr1 3A

<400>  7
gttatcagga tgcaaatgca gctgctgagg agtttgctcg cc                          42


<210>  8
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer of Arr1 3A

<400>  8
ggcgagcaaa ctcctcagca gctgcatttg catcctgata ac                          42


<210>  9
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer of K261Q

<400>  9
cgagtgatta ttacgtccag cccgtggcta tggag                                  35


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer of K261Q

<400>  10
ctccatagcc acgggctgga cgtaataatc actcg                                  35


<210>  11
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer of Q332K

<400>  11
gaatcctggt gtcttacaag atcaaggtga agctcac                                37


<210>  12
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer of Q332K

<400>  12
gtgagcttca ccttgatctt gtaagacacc aggattcc                               38


<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer of E350H

<400>  13
gagagctcac ctccagtcac gtcgccactg aggtcc                                 36


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer of E350H

<400>  14
ggacctcagt ggcgacgtga ctggaggtga gctctc                                 36


<210>  15
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer of Arr

<400>  15
ccaggtggcc ccggctacgc gtgcagccag cgggaagacc agc                         43


<210>  16
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer of Arr

<400>  16
caggaattcg atatcaagca ccggtttact catcaacgtc attcttgtct ctc              53


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F1

<400>  17

Gly Gly Gly Gly Ser 
1               5   


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2

<400>  18

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F3

<400>  19

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R1

<400>  20

Ala Glu Ala Ala Ala Lys Ala 
1               5           


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R2

<400>  21

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10          


<210>  22
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R3

<400>  22

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Ala 
    


<210>  23
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SF-1

<400>  23

Gly Gly Gly Gly Ser Ala Glu Ala Ala Ala Lys Ala Gly Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  24
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SF-2

<400>  24

Gly Gly Gly Gly Ser Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Ala Gly Gly Gly Gly Ser 
            20          


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SF-3

<400>  25

Gly Gly Gly Gly Ser Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Gly Gly Gly Gly Ser 
            20                  25          


<210>  26
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SF-4

<400>  26

Gly Gly Gly Gly Ser Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Gly Gly Gly Gly Ser 
            20                  25                  30          


<210>  27
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SF-5

<400>  27

Gly Gly Gly Gly Ser Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
            20                  25                  30          


Gly Gly Gly Gly Ser 
        35          


<210>  28
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GSG)4

<400>  28

Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10          


