                         SEQUENCE LISTING

<110>  BioVentures, LLC
       Erming, Tian
       Zangari, Maurizio
 
<120>  BIOLOGICALLY SYNTHESIZED HYDROXYAPATITE FOR BONE REGENERATION AND TISSUE ENGINEERING

<130>  169852.00075

<150>  62/915,843
<151>  2019-10-16

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  524
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(524)
<223>  Human tissue nonspecific alkaline phosphatase (ALPL)

<400>  1

Met Ile Ser Pro Phe Leu Val Leu Ala Ile Gly Thr Cys Leu Thr Asn 
1               5                   10                  15      


Ser Leu Val Pro Glu Lys Glu Lys Asp Pro Lys Tyr Trp Arg Asp Gln 
            20                  25                  30          


Ala Gln Glu Thr Leu Lys Tyr Ala Leu Glu Leu Gln Lys Leu Asn Thr 
        35                  40                  45              


Asn Val Ala Lys Asn Val Ile Met Phe Leu Gly Asp Gly Met Gly Val 
    50                  55                  60                  


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Leu His His Asn 
65                  70                  75                  80  


Pro Gly Glu Glu Thr Arg Leu Glu Met Asp Lys Phe Pro Phe Val Ala 
                85                  90                  95      


Leu Ser Lys Thr Tyr Asn Thr Asn Ala Gln Val Pro Asp Ser Ala Gly 
            100                 105                 110         


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Ala Asn Glu Gly Thr Val 
        115                 120                 125             


Gly Val Ser Ala Ala Thr Glu Arg Ser Arg Cys Asn Thr Thr Gln Gly 
    130                 135                 140                 


Asn Glu Val Thr Ser Ile Leu Arg Trp Ala Lys Asp Ala Gly Lys Ser 
145                 150                 155                 160 


Val Gly Ile Val Thr Thr Thr Arg Val Asn His Ala Thr Pro Ser Ala 
                165                 170                 175     


Ala Tyr Ala His Ser Ala Asp Arg Asp Trp Tyr Ser Asp Asn Glu Met 
            180                 185                 190         


Pro Pro Glu Ala Leu Ser Gln Gly Cys Lys Asp Ile Ala Tyr Gln Leu 
        195                 200                 205             


Met His Asn Ile Arg Asp Ile Asp Val Ile Met Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Tyr Pro Lys Asn Lys Thr Asp Val Glu Tyr Glu Ser Asp Glu 
225                 230                 235                 240 


Lys Ala Arg Gly Thr Arg Leu Asp Gly Leu Asp Leu Val Asp Thr Trp 
                245                 250                 255     


Lys Ser Phe Lys Pro Arg Tyr Lys His Ser His Phe Ile Trp Asn Arg 
            260                 265                 270         


Thr Glu Leu Leu Thr Leu Asp Pro His Asn Val Asp Tyr Leu Leu Gly 
        275                 280                 285             


Leu Phe Glu Pro Gly Asp Met Gln Tyr Glu Leu Asn Arg Asn Asn Val 
    290                 295                 300                 


Thr Asp Pro Ser Leu Ser Glu Met Val Val Val Ala Ile Gln Ile Leu 
305                 310                 315                 320 


Arg Lys Asn Pro Lys Gly Phe Phe Leu Leu Val Glu Gly Gly Arg Ile 
                325                 330                 335     


Asp His Gly His His Glu Gly Lys Ala Lys Gln Ala Leu His Glu Ala 
            340                 345                 350         


Val Glu Met Asp Arg Ala Ile Gly Gln Ala Gly Ser Leu Thr Ser Ser 
        355                 360                 365             


Glu Asp Thr Leu Thr Val Val Thr Ala Asp His Ser His Val Phe Thr 
    370                 375                 380                 


Phe Gly Gly Tyr Thr Pro Arg Gly Asn Ser Ile Phe Gly Leu Ala Pro 
385                 390                 395                 400 


Met Leu Ser Asp Thr Asp Lys Lys Pro Phe Thr Ala Ile Leu Tyr Gly 
                405                 410                 415     


Asn Gly Pro Gly Tyr Lys Val Val Gly Gly Glu Arg Glu Asn Val Ser 
            420                 425                 430         


Met Val Asp Tyr Ala His Asn Asn Tyr Gln Ala Gln Ser Ala Val Pro 
        435                 440                 445             


Leu Arg His Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ser Lys 
    450                 455                 460                 


Gly Pro Met Ala His Leu Leu His Gly Val His Glu Gln Asn Tyr Val 
465                 470                 475                 480 


Pro His Val Met Ala Tyr Ala Ala Cys Ile Gly Ala Asn Leu Gly His 
                485                 490                 495     


Cys Ala Pro Ala Ser Ser Ala Gly Ser Leu Ala Ala Gly Pro Leu Leu 
            500                 505                 510         


Leu Ala Leu Ala Leu Tyr Pro Leu Ser Val Leu Phe 
        515                 520                 


<210>  2
<211>  533
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<222>  (1)..(533)
<223>  Bovine intestine alkaline phosphatase (CIP)

<400>  2

Met Gln Gly Ala Cys Val Leu Leu Leu Leu Gly Leu His Leu Gln Leu 
1               5                   10                  15      


Ser Leu Gly Leu Val Pro Val Glu Glu Glu Asp Pro Ala Phe Trp Asn 
            20                  25                  30          


Arg Gln Ala Ala Gln Ala Leu Asp Val Ala Lys Lys Leu Gln Pro Ile 
        35                  40                  45              


Gln Thr Ala Ala Lys Asn Val Ile Leu Phe Leu Gly Asp Gly Met Gly 
    50                  55                  60                  


Val Pro Thr Val Thr Ala Thr Arg Ile Leu Lys Gly Gln Met Asn Gly 
65                  70                  75                  80  


Lys Leu Gly Pro Glu Thr Pro Leu Ala Met Asp Gln Phe Pro Tyr Val 
                85                  90                  95      


Ala Leu Ser Lys Thr Tyr Asn Val Asp Arg Gln Val Pro Asp Ser Ala 
            100                 105                 110         


Gly Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Gly Asn Tyr Arg Thr 
        115                 120                 125             


Ile Gly Val Ser Ala Ala Ala Arg Tyr Asn Gln Cys Lys Thr Thr Arg 
    130                 135                 140                 


Gly Asn Glu Val Thr Ser Val Met Asn Arg Ala Lys Lys Ala Gly Lys 
145                 150                 155                 160 


Ser Val Gly Val Val Thr Thr Thr Arg Val Gln His Ala Ser Pro Ala 
                165                 170                 175     


Gly Ala Tyr Ala His Thr Val Asn Arg Asn Trp Tyr Ser Asp Ala Asp 
            180                 185                 190         


Leu Pro Ala Asp Ala Gln Met Asn Gly Cys Gln Asp Ile Ala Ala Gln 
        195                 200                 205             


Leu Val Asn Asn Met Asp Ile Asp Val Ile Leu Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Phe Pro Val Gly Thr Pro Asp Pro Glu Tyr Pro Asp Asp Ala 
225                 230                 235                 240 


Ser Val Asn Gly Val Arg Lys Arg Lys Gln Asn Leu Val Gln Ala Trp 
                245                 250                 255     


Gln Ala Lys His Gln Gly Ala Gln Tyr Val Trp Asn Arg Thr Ala Leu 
            260                 265                 270         


Leu Gln Ala Ala Asp Asp Ser Ser Val Thr His Leu Met Gly Leu Phe 
        275                 280                 285             


Glu Pro Ala Asp Met Lys Tyr Asn Val Gln Gln Asp His Thr Lys Asp 
    290                 295                 300                 


Pro Thr Leu Gln Glu Met Thr Glu Val Ala Leu Arg Val Val Ser Arg 
305                 310                 315                 320 


Asn Pro Arg Gly Phe Tyr Leu Phe Val Glu Gly Gly Arg Ile Asp His 
                325                 330                 335     


Gly His His Asp Asp Lys Ala Tyr Met Ala Leu Thr Glu Ala Gly Met 
            340                 345                 350         


Phe Asp Asn Ala Ile Ala Lys Ala Asn Glu Leu Thr Ser Glu Leu Asp 
        355                 360                 365             


Thr Leu Ile Leu Val Thr Ala Asp His Ser His Val Phe Ser Phe Gly 
    370                 375                 380                 


Gly Tyr Thr Leu Arg Gly Thr Ser Ile Phe Gly Leu Ala Pro Ser Lys 
385                 390                 395                 400 


Ala Leu Asp Ser Lys Ser Tyr Thr Ser Ile Leu Tyr Gly Asn Gly Pro 
                405                 410                 415     


Gly Tyr Ala Leu Gly Gly Gly Ser Arg Pro Asp Val Asn Asp Ser Thr 
            420                 425                 430         


Ser Glu Asp Pro Ser Tyr Gln Gln Gln Ala Ala Val Pro Gln Ala Ser 
        435                 440                 445             


Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ala Arg Gly Pro Gln 
    450                 455                 460                 


Ala His Leu Val His Gly Val Glu Glu Glu Thr Phe Val Ala His Ile 
465                 470                 475                 480 


Met Ala Phe Ala Gly Cys Val Glu Pro Tyr Thr Asp Cys Asn Leu Pro 
                485                 490                 495     


Ala Pro Thr Thr Ala Thr Ser Ile Pro Asp Ala Ala His Leu Ala Ala 
            500                 505                 510         


Ser Pro Pro Pro Leu Ala Leu Leu Ala Gly Ala Met Leu Leu Leu Leu 
        515                 520                 525             


Ala Pro Thr Leu Tyr 
    530             


<210>  3
<211>  404
<212>  PRT
<213>  Pandalus borealis


<220>
<221>  MISC_FEATURE
<222>  (1)..(404)
<223>  Shrimp alkaline phosphatase (rSAP)

<400>  3

Met Ile Gly Arg Thr Thr Phe Ile Ala Leu Phe Val Lys Val Leu Thr 
1               5                   10                  15      


Ile Trp Ser Phe Thr Lys Gly Glu Asp Cys Val Trp Asp Asn Asp Val 
            20                  25                  30          


Asp Tyr Pro Glu Tyr Pro Pro Leu Ile Leu Asp Ser Ser Phe Gln Leu 
        35                  40                  45              


Val Leu Pro Val Leu Glu Gly Asp Gln Arg Ile Thr Ser Val Gln Ser 
    50                  55                  60                  


Gly Ser Glu Leu Ile Leu Ala Cys Pro Gly Arg Glu Ile Ser Ala Leu 
65                  70                  75                  80  


Gly Ser Glu Asp Ala Gln Ala Thr Cys Leu Gly Gly Lys Leu Val Glu 
                85                  90                  95      


Val Asp Gly Lys Glu Trp Asn Ile Val Glu Leu Gly Cys Thr Lys Met 
            100                 105                 110         


Ala Ser Glu Thr Ile His Arg Asn Leu Gly Gln Cys Gly Asp Gln Asp 
        115                 120                 125             


Leu Gly Ile Tyr Glu Val Ile Gly Phe Asp Leu Pro Thr Thr Gly His 
    130                 135                 140                 


Phe Tyr Glu Leu Ile Arg Val Cys Phe Asp Pro Ala Asn Glu Thr Thr 
145                 150                 155                 160 


Ile Phe Ser Glu Asn Ile Val His Gly Ala Ser Ile Ala Ala Lys Asp 
                165                 170                 175     


Ile Asp Pro Gly Arg Pro Ser Phe Lys Thr Ser Thr Gly Phe Phe Ser 
            180                 185                 190         


Val Ser Met Ile Ser Val Tyr Ser Gln Arg Asn Gln Leu Glu Leu Met 
        195                 200                 205             


Lys Asn Leu Leu Gly Asp Asp Glu Leu Ala Ala Thr Ile Ile Asp Pro 
    210                 215                 220                 


Ser Lys Gln Phe Tyr Phe Ala Lys Gly His Met Ala Pro Asp Ala Asp 
225                 230                 235                 240 


Phe Val Thr Val Val Glu Gln Asp Ala Thr Tyr Tyr Tyr Ile Asn Ala 
                245                 250                 255     


Leu Pro Gln Trp Gln Ala Phe Asn Asn Gly Asn Trp Lys Tyr Leu Glu 
            260                 265                 270         


Tyr Asp Thr Arg Asp Leu Ala Glu Lys His Gly Thr Asp Leu Thr Val 
        275                 280                 285             


Tyr Ser Gly Gly Trp Gly Val Leu Glu Leu Glu Asp Ile Asn Gly Asn 
    290                 295                 300                 


Pro Val Glu Ile Tyr Leu Gly Leu Ala Gln Asp Lys Lys Val Val Pro 
305                 310                 315                 320 


Ala Pro Ala Leu Thr Trp Lys Val Ile Tyr Glu Lys Asp Thr Asn Arg 
                325                 330                 335     


Ala Ala Ala Ile Val Gly Ile Asn Asn Pro His Ile Thr Thr Ala Pro 
            340                 345                 350         


Glu Pro Leu Cys Thr Asp Ile Cys Ser Ser Leu Thr Trp Leu Asp Phe 
        355                 360                 365             


Asp Phe Gly Asp Leu Val His Gly Tyr Thr Tyr Cys Cys Ser Val Ala 
    370                 375                 380                 


Asp Leu Arg Ala Ala Ile Pro Asn Val Pro Asp Leu Gly Asp Val Asp 
385                 390                 395                 400 


Ile Leu Asp Glu 
                


