                         SEQUENCE LISTING

<110>  Ramot at Tel-Aviv University Ltd.
 
<120>  ROP - DEFICIENT PLANTS HAVING HIGH WATER USE EFFICIENCY

<130>  RAMOT-093 PCT

<150>  62/940,265
<151>  2019-11-26

<150>  63/008,754
<151>  2020-04-12

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  210
<212>  PRT
<213>  Solanum lycopersicum

<400>  1

Met Ala Ser Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp 
1               5                   10                  15      


Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys 
            20                  25                  30          


Phe Pro Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn 
        35                  40                  45              


Val Val Val Glu Gly Thr Thr Val Asn Leu Gly Leu Trp Asp Thr Ala 
    50                  55                  60                  


Gly Gln Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala 
65                  70                  75                  80  


Asp Val Phe Val Leu Ala Phe Ser Leu Val Ser Arg Ala Ser Tyr Glu 
                85                  90                  95      


Asn Ile Leu Lys Lys Trp Ile Pro Glu Leu Gln His Tyr Ala Pro Gly 
            100                 105                 110         


Ile Pro Val Val Leu Ala Gly Thr Lys Leu Asp Leu Arg Glu Asp Lys 
        115                 120                 125             


His Phe Leu Ala Asp His Pro Gly Leu Val Pro Val Thr Thr Ala Gln 
    130                 135                 140                 


Gly Glu Glu Leu Arg Lys Gln Ile Gly Ala Ala Tyr Tyr Ile Glu Cys 
145                 150                 155                 160 


Ser Ser Lys Thr Gln Gln Asn Val Lys Ala Val Phe Asp Ala Ala Ile 
                165                 170                 175     


Lys Val Val Ile Lys Pro Pro Gln Lys Gln Lys Glu Lys Lys Lys Gln 
            180                 185                 190         


Arg Arg Gly Cys Leu Met Asn Val Met Cys Gly Arg Lys Leu Val Cys 
        195                 200                 205             


Leu Lys 
    210 


<210>  2
<211>  633
<212>  DNA
<213>  Solanum lycopersicum

<400>  2
atggcctcaa gtgcttcaag attcatcaaa tgtgtcacgg ttggtgatgg ggctgttggc       60

aagacttgta tgcttatttg ctataccagt aacaagtttc ccactgacta tgttccgacg      120

gtgtttgaca acttcagcgc caatgtggtt gttgaaggga ccacagtaaa tttaggtctt      180

tgggatactg caggacaaga agattataac agattaaggc cactgagcta ccgaggagca      240

gatgtttttg tcctagcgtt ctccttggtg agtcgtgcaa gctatgaaaa catacttaag      300

aagtggattc ccgaacttca gcattatgct cctggaattc cggtggtatt agctggcacc      360

aaacttgatc ttcgtgagga taagcacttc ttggctgatc atcctggatt agttcctgtc      420

accactgcgc agggagagga gctgcggaaa caaattggtg ctgcctatta tatcgaatgt      480

agctctaaaa cacaacagaa tgtgaaagct gtatttgatg ctgcaatcaa ggtcgtcatc      540

aagccaccac aaaagcaaaa ggagaagaaa aaacaacgtc ggggatgtct catgaatgtg      600

atgtgcggaa ggaagctcgt ttgtttaaag tga                                   633


<210>  3
<211>  373
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  3
taggagtggg ggtgggggag aaacttaccc taaggaaaat gaaatggtca cctaatcaac       60

tttggggaat gttagtagct cagtatggtt aactgaactt tcacattgtt ggtgaattgt      120

tggtgagggt ttgattccac accttggaat cccctcccca cttttccctt ttaaaaaaag      180

accgtatcag ctagaaagaa cagaatgtta tggttaactc gtgtgattca ggagtatttg      240

ttgagtttct ttcctgagct tgaacttcag ttttctgagt ggttgtatat gttcaaacca      300

ggatctatgt tccaacggtg tttgacaact tcagcgccaa tgtgggtgtt gaagggacca      360

cagtaaattt agg                                                         373


<210>  4
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  4
gggggtgggg gagaaactta ccctaaggaa aatgaaatgg tcacctaatc aactttgggg       60

aatgttagta gctcagtatg gttaactgaa ctttcacatt gttggtgaat tgttggtgag      120

ggtttgattc cacaccttgg aatcccctcc ccacttttcc cttttaaaaa aagaccgtat      180

cagctagaaa gaacagaatg ttatggttaa ctcgtgtgat tcaggagtat ttgttgagtt      240

tctttcctga gcttgaactt cagttttctg agtggttgta tatgttcaaa ccaggattcc      300

aacggtgttt gacaacttca gcgccaatgt ggttgttgaa gggaccacag taaatttagg      360


<210>  5
<211>  506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  5
ggcgttctcc ttggtgagtg gggcaagcta tgaaaacata cttaagaagg tatttctgtt       60

gaaaacagtg aactttgtga gtttgtatca tatgtattta agattgctaa aatcccattt      120

ttggtcagtg gattcccgaa cttcagcatt atgctcctgg aaccggtggt attagctggc      180

accaaacttg gtaagtattt aacatgttcc ctgaagctaa gttataacaa atgctgattc      240

ttgaatgtaa ctgcttcagt ttaatgtcac tttgttctat gatgtgcttg ctaaaatgct      300

aatatgtcta cttataaaaa ataaatataa aatgttgatg tgttgaatgt agctgtactt      360

tgtagttttc agagtttatt gtcgctaata ctgtattcaa aagcgtaaat aacttaaggg      420

gattgcacga tggaggtaaa aagacgtgaa tacagtataa cttaaggaga ttgcatgacc      480

ggagggaggt ctagagatgg aactgg                                           506


<210>  6
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  6
atggcctcaa gtgcttcaag attcatcaaa tgtgtcacgg ttggtgatgg ggctgttggc       60

aagacttgta tgcttatttg ctataccagt aacaagtttc ccactgacta tgttccgacg      120

gtgtttgaca acttcagcgc caatgtggtt gttgaaggga ccacagtaaa tttaggtctt      180

tgggatactg caggacaaga agattataac agattaaggc cactgagcta ccgaggagca      240

gatgtttttg tcctagcgtt ctccttggtg agtcgtgcaa gctatgaaaa catacttaag      300

aaggtatttc tgttgaaaac agtgaacttt gtgagtttgt atcatatgta tttaagattg      360

ctaaaatccc atttttggtc agtggattcc cgaacttcag cattatgctc ctggattccg      420

gtggtattag ctggcaccaa acttggtaag tatttaacat gttccctgaa gctaagttat      480

aacaaatgct gattcttgaa tgtaactgct tcagtttaat gtcactttgt tctatgatgt      540

gcttgctaaa atgctaatat gtctacttat aaaaaataaa tataaaatgt tgatgtgttg      600

aatgtagctg tactttgtag ttttcagagt ttattgtcgc taatactgta ttcaaaagcg      660

taaataactt aaggggattg cacgatggag gtaaaaagac gtgaatacag tataacttaa      720

ggagattgca tgaccggagg gaggtctaga gatggaactg ga                         762


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  7

Met Ala Ser Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp 
1               5                   10                  15      


Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys 
            20                  25                  30          


Phe Pro Thr Asp Leu Cys Ser Asp Gly Val 
        35                  40          


<210>  8
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  8

Met Ala Ser Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp 
1               5                   10                  15      


Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys 
            20                  25                  30          


Phe Pro Thr Asp Ser Asp Gly Val 
        35                  40  


<210>  9
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  9

Met Ala Ser Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp 
1               5                   10                  15      


Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys 
            20                  25                  30          


Phe Pro Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn 
        35                  40                  45              


Val Val Val Glu Gly Thr Thr Val Asn Leu Gly Leu Trp Asp Thr Ala 
    50                  55                  60                  


Gly Gln Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala 
65                  70                  75                  80  


Asp Val Phe Val Leu Ala Phe Ser Leu Val Ser Arg Ala Ser Tyr Glu 
                85                  90                  95      


Asn Ile Leu Lys Lys Trp Ile Pro Glu Leu Gln His Tyr Ala Pro Gly 
            100                 105                 110         


Thr Gly Gly Ile Ser Trp His Gln Thr 
        115                 120     


<210>  10
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SlROP9 Mutant

<400>  10

Met Ala Ser Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp 
1               5                   10                  15      


Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys 
            20                  25                  30          


Phe Pro Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn 
        35                  40                  45              


Val Val Val Glu Gly Thr Thr Val Asn Leu Gly Leu Trp Asp Thr Ala 
    50                  55                  60                  


Gly Gln Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala 
65                  70                  75                  80  


Asp Val Phe Val Leu Ala Phe Ser Leu Val Ser Arg Ala Ser Tyr Glu 
                85                  90                  95      


Asn Ile Leu Lys Lys Trp Ile Pro Glu Leu Gln His Tyr Ala Pro Gly 
            100                 105                 110         


Phe Arg Trp Tyr 
        115     


<210>  11
<211>  373
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Variant

<400>  11
gtaggagtgg gggtggggga gaaacttacc ctaaggaaaa tgaaatggtc acctaatcaa       60

ctttggggaa tgttagtagc tcagtatggt taactgaact ttcacattgt tggtgaattg      120

ttggtgaggg tttgattcca caccttggaa tcccctcccc acttttccct tttaaaaaaa      180

gaccgtatca gctagaaaga acagaatgtt atggttaact cgtgtgattc aggagtattt      240

gttgagtttc tttcctgagc ttgaacttca gttttctgag tggttgtata tgttcaaacc      300

aggactatgt tccaacggtg tttgacaact tcagcgccaa tgtggttgtt gaagggacca      360

cagtaaattt agg                                                         373


<210>  12
<211>  506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SlROP9 Variant


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, t or u

<400>  12
gttctcnttg gtgantgcgt gcaagctatg aaaacatact taagaaggta tttctgttga       60

aaacagtgaa ctttgtgagt ttgtatcata tgtatttaag attgctaaaa tcccattttt      120

ggtcagtgga ttcccgaact tcagcattat gctcctggaa ttccggtggt attagctggc      180

accaaacttg gtaagtattt aacatgttcc ctgaagctaa gttataacaa atgctgattc      240

ttgaatgtaa ctgcttcagt ttaatgtcac tttgttctat gatgtgcttg ctaaaatgct      300

aatatgtcta cttataaaaa ataaatataa aatgttgatg tgttgaatgt agctgtactt      360

tgtagttttc agagtttatt gtcgctaata ctgtattcaa aagcgtaaat aacttaaggg      420

gattgcacga tggaggtaaa aagacgtgaa tacagtataa cttaaggaga ttgcatgacc      480

ggagggaggt ctagagatgg aactgg                                           506


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  13
cacattggtc aggccggtat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  14
atctggccat caggctgaat                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  15
gtgtcacggt tggtgatggg g                                                 21


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  16
ctgctcctcg gtagctcagt gg                                                22


<210>  17
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  17
ggggacagct ttcttgtaca aagtggccgc ctcaagtgct tcaagattca t                51


<210>  18
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  18
ggggacaact ttgtataata aagttgttca ctttaaacaa acgagcttcc ttccg            55


<210>  19
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  19
ggggacaagt ttgtacaaaa aagcaggctt aatgccaaga ttaaggggat cagatatgct       60

tcaaagg                                                                 67


<210>  20
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  20
gggaccactt tgtacaagaa agctgggtgt ttgtgtccct tctttctcca caggtatcca       60

agc                                                                     63


<210>  21
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  21
ggggacaagt ttgtacaaaa aagcaggctt aatgccaaga tcaaggggat cagaaatgcc       60


<210>  22
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  22
ggggaccact ttgtacaaga aagctgggtg tttgtggccc ttcttcttcc agaggtcacc       60


<210>  23
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  23
tgtggtctca attgcaccgt cggaacatag tccgttttag agctagaaat agcaag           56


<210>  24
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  24
tgtggtctca attgctaata ccaccggaat tccgttttag agctagaaat agcaag           56


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  25
tgtggtctca agcgtaatgc caactttgta c                                      31


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  26
gatccatgaa cccagctgac gg                                                22


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  27
ctacctgagc acccagtccg                                                   20


<210>  28
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  28
gtcgccgtcc agctcgac                                                     18


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide - Primer

<400>  29
cgggggaggc agagggtttc c                                                 21


<210>  30
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<222>  (15)..(33)
<223>  n is a, c, g, t or u

<400>  30
tgtggtctca attgnnnnnn nnnnnnnnnn nnngttttag agctagaaat agcaag           56


