                         SEQUENCE LISTING

<110>  DendroCyte BioTech Pty Ltd
 
<120>  Antigen Loading of Antigen Presenting Cells

<130>  P112319.PCT

<150>  AU2019904614
<151>  2019-12-05

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DCR-2 VH region

<400>  1

Met Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Pro Leu Lys Leu 
1               5                   10                  15      


Ser Cys Ala Ala Ser Gly Phe Gly Phe Ser Gly Ser Trp Met Ser Trp 
            20                  25                  30          


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Gln Ile Asn 
        35                  40                  45              


Pro Asp Ser Ser Thr Ile Asn Tyr Thr Pro Ser Leu Lys Asp Lys Phe 
    50                  55                  60                  


Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Ile Asn 
65                  70                  75                  80  


Lys Val Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys Ala Arg Arg Gly 
                85                  90                  95      


Phe Phe Glu Gly Tyr Ser Ala Trp Phe Ala Tyr Trp 
            100                 105             


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VH of DCR-2

<400>  2

Gly Phe Gly Phe Ser Gly Ser Trp 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VH of DCR-2

<400>  3

Ile Asn Pro Asp Ser Ser Thr Ile 
1               5               


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VH of DCR-2

<400>  4

Ala Arg Arg Gly Phe Phe Glu Gly Tyr Ser Ala Trp Phe Ala Tyr 
1               5                   10                  15  


<210>  5
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DCR-2

<400>  5

Ile Leu Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly Asp 
1               5                   10                  15      


Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp Val 
            20                  25                  30          


Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Ser Leu Leu Ile Tyr 
        35                  40                  45              


Tyr Ala Ser Asn Arg Asn Thr Gly Val Pro Asp Arg Phe Thr Gly Ser 
    50                  55                  60                  


Gly Tyr Glu Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala Glu 
65                  70                  75                  80  


Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Thr Ser Pro Trp Thr 
                85                  90                  95      


Phe Gly Gly Gly 
            100 


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VL of DCR-2

<400>  6

Gln Ser Val Ser Asn Asp 
1               5       


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VL of DCR-2

<400>  7

Tyr Ala Ser 
1           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VL of DCR-2

<400>  8

Gln Gln Asp Tyr Thr Ser Pro Trp Thr 
1               5                   


<210>  9
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH region of DCR-2

<400>  9
atggagtctg gaggtggcct ggtgcagcct ggaggacccc tgaaactctc ctgtgcagcc       60

tcaggattcg gttttagtgg atcttggatg agttgggtcc ggcaggctcc agggaaaggg      120

ctagaatgga ttggacaaat taatccagat agcagtacga taaattatac accatctcta      180

aaggataaat tcatcatctc cagagacaac gccaaaaata ccctgtacct gcaaattaac      240

aaagtgagat ctgaggacac agccctttat tactgtgcaa gacgggggtt ctttgaaggt      300

tactccgcct ggtttgctta ctgg                                             324


<210>  10
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL region of DCR-2

<400>  10
attttgatga cccagactcc caaattcctg cttgtatcag caggagacag ggtgaccata       60

acctgcaagg ccagtcagag tgtgagtaat gatgtagctt ggtaccaaca gaagccaggg      120

cagtctcctt cactcctgat atactatgca tccaatcgca acactggagt ccctgatcgc      180

ttcactggca gtggatatga gacggatttc actttcacca tcagcactgt gcaggctgaa      240

gacctggcag tttatttctg tcagcaggat tatacctctc cgtggacgtt cggtggaggc      300


<210>  11
<211>  1422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised chimeric heavy chain

<400>  11
atggtcctga gcctgctgta cctgctgaca gctctgcctg gcatcctgtc tgaggtccag       60

ctgcaagagt ctggccccat ggaatctggc ggaggattgg ttcaacctgg cggccctctg      120

aagctgtctt gtgccgcttc tggcttcggc ttctccggct cttggatgtc ctgggtccga      180

caggctcctg gcaaaggcct ggaatggatc ggccagatca accccgactc ctccaccatc      240

aactacaccc ctagcctgaa ggacaagttc atcatctccc gggacaacgc caagaacacc      300

ctgtacttgc agatcaacaa agtgcggagc gaggacaccg ctctgtacta ctgtgccaga      360

cggggcttct tcgagggcta ctctgcttgg tttgcctact ggggccaggg cacactggtc      420

acagtttctg ccgcctctac caagggaccc agcgttttcc ctctggctcc atcctccaag      480

tctacctctg gcggaacagc tgctctgggc tgcctggtca aggactactt tcctgagcca      540

gtgaccgtgt cctggaactc tggcgctctg acatctggcg tgcacacctt tccagctgtg      600

ctgcagtcct ccggcctgta ctctctgtcc tctgtcgtga ccgtgccttc cagctctctg      660

ggaacccaga cctacatctg caatgtgaac cacaagcctt ccaacaccaa ggtggacaag      720

aaggtggaac ccaagtcctg cgacaagacc cacacctgtc ctccatgtcc tgctccagaa      780

ctgctcggcg gaccttccgt gttcctgttt cctccaaagc ctaaggacac cctgatgatc      840

tctcggaccc ctgaagtgac ctgcgtggtg gtggatgtgt ctcacgagga tcccgaagtg      900

aagttcaatt ggtacgtgga cggcgtggaa gtgcacaatg ccaagaccaa gcctagagag      960

gaacagtaca actccaccta tagagtggtg tccgtgctga ccgtgctgca ccaggattgg     1020

ctgaacggca aagagtacaa gtgcaaggtg tccaacaagg ccctgcctgc tcctatcgaa     1080

aagaccatct ccaaggccaa gggccagcct agggaacccc aggtttacac cttgcctcca     1140

tctcgggacg agctgaccaa gaaccaggtg tccctgacct gtctcgtgaa gggcttctac     1200

ccctccgata tcgccgtgga atgggagtct aatggccagc ctgagaacaa ctacaagaca     1260

acccctcctg tgctggactc cgacggctca ttcttcctgt actccaagct gacagtggac     1320

aagtccagat ggcagcaggg caacgtgttc tcctgctccg tgatgcacga ggccctgcac     1380

aatcactaca cccagaagtc cctgtctctg tcccctggca ag                        1422


<210>  12
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised chimeric light chain

<400>  12
atggactctc aggcccaggt gctgatgctg ctgctgttgt gggtgtccgg cacctgtggc       60

gacatcctga tgacccagac tcctaagttc ctgctggtgt ctgccggcga cagagtgacc      120

atcacatgca aggcctctca gtccgtgtcc aacgacgtgg cctggtatca gcagaagcct      180

ggccagtctc ctagcctgct gatctactac gcctccaaca gaaacaccgg cgtgcccgat      240

agattcaccg gctctggcta cgagacagac ttcaccttca ccatctccac cgtgcaggcc      300

gaggatctgg ccgtgtactt ctgccagcaa gactacacct ctccatggac ctttggcgga      360

ggcaccaagc tggaaatcaa gcggacagtg gccgctcctt ccgtgttcat cttcccacct      420

tccgacgagc agctgaagtc tggcacagcc tctgtcgtgt gcctgctgaa caacttctac      480

cctcgggaag ccaaggtgca gtggaaggtg gacaatgccc tgcagtccgg caactcccaa      540

gagtctgtga ccgagcagga ctccaaggac agcacctaca gcctgtcctc cacactgacc      600

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccatcagggc      660

ctgtctagcc ctgtgaccaa gtctttcaac cggggcgagt gc                         702


<210>  13
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain encoded by codon optimised nucleotide sequence

<400>  13

Met Val Leu Ser Leu Leu Tyr Leu Leu Thr Ala Leu Pro Gly Ile Leu 
1               5                   10                  15      


Ser Glu Val Gln Leu Gln Glu Ser Gly Pro Met Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Pro Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Gly Phe Ser Gly Ser Trp Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Gly Gln Ile Asn Pro Asp Ser Ser Thr Ile 
65                  70                  75                  80  


Asn Tyr Thr Pro Ser Leu Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Thr Leu Tyr Leu Gln Ile Asn Lys Val Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Leu Tyr Tyr Cys Ala Arg Arg Gly Phe Phe Glu Gly Tyr Ser 
        115                 120                 125             


Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
    130                 135                 140                 


Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
145                 150                 155                 160 


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
                165                 170                 175     


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
            180                 185                 190         


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
        195                 200                 205             


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
    210                 215                 220                 


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
225                 230                 235                 240 


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 


<210>  14
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain encoded by codon optimised nucleotide sequence

<400>  14

Met Asp Ser Gln Ala Gln Val Leu Met Leu Leu Leu Leu Trp Val Ser 
1               5                   10                  15      


Gly Thr Cys Gly Asp Ile Leu Met Thr Gln Thr Pro Lys Phe Leu Leu 
            20                  25                  30          


Val Ser Ala Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser 
        35                  40                  45              


Val Ser Asn Asp Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Ser Leu Leu Ile Tyr Tyr Ala Ser Asn Arg Asn Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Tyr Glu Thr Asp Phe Thr Phe Thr Ile Ser 
                85                  90                  95      


Thr Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr 
            100                 105                 110         


Thr Ser Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Met Asp Ser Gln Ala Gln 
225                 230                 1               5       


Val Leu Met Leu Leu Leu Leu Trp Val Ser Gly Thr Cys Gly Asp Ile 
            10                  15                  20          


Leu Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly Asp Arg 
        25                  30                  35              


Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp Val Ala 
    40                  45                  50                  


Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Ser Leu Leu Ile Tyr Tyr 
55                  60                  65                  70  


Ala Ser Asn Arg Asn Thr Gly Val Pro Asp Arg Phe Thr Gly Ser Gly 
                75                  80                  85      


Tyr Glu Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala Glu Asp 
            90                  95                  100         


Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Thr Ser Pro Trp Thr Phe 
        105                 110                 115             


Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser 
    120                 125                 130                 


Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
135                 140                 145                 150 


Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val 
                155                 160                 165     


Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser 
            170                 175                 180         


Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr 
        185                 190                 195             


Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys 
    200                 205                 210                 


Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn 
215                 220                 225                 230 


Arg Gly Glu Cys 
                


<210>  15
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanised VH of DCR-2

<400>  15

Met Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu 
1               5                   10                  15      


Ser Cys Ala Ala Ser Gly Phe Gly Phe Ser Gly Ser Trp Met Ser Trp 
            20                  25                  30          


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asn Ile Asn 
        35                  40                  45              


Pro Asp Ser Ser Thr Ile Tyr Tyr Val Asp Ser Val Lys Gly Arg Phe 
    50                  55                  60                  


Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn 
65                  70                  75                  80  


Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg Gly 
                85                  90                  95      


Phe Phe Glu Gly Tyr Ser Ala Trp Phe Ala Tyr Trp 
            100                 105             


<210>  16
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanised light chain variable region of DCR-2

<400>  16

Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
1               5                   10                  15      


Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp Leu 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
        35                  40                  45              


Tyr Ala Ser Asn Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Asp Tyr Thr Ser Pro Trp Thr 
                85                  90                  95      


Phe Gly Gly Gly 
            100 


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DCR-2 heavy joining region

<400>  17

Gly Gln Gly Thr Leu Val Thr Val 
1               5               


