                         SEQUENCE LISTING

<110>  VANDERBILT UNIVERSITY
 
<120>  METHODS OF SYNTHESIZING MRNA AND FUNCTIONAL PROTEINS FROM 
       SYNTHETIC DOUBLE STRANDED DNA

<130>  10644-101WO1

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  998
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1
atttaggtga cactatagaa tgaccctgct tgctcaactc tacgtctttg tttcgttttc       60

tgttctgcgc cgttacagat ccaagctgtg accggcgcct acctgagatc accggtcacc      120

atggaaatca aggtgctgtt tgccctcatc tgtattgctg ttgctgaggc aaaacccact      180

gaaatcaatg aagacctcaa tatagctgct gtggcctcca actttgccac cacagatctt      240

gagactgacc tgttcaccaa ctgggagacc atgaatgtga ttagcactga cacagagcag      300

gtgaacacag atgctgacag gggcaagctg cctggcaaaa aactcccccc agatgtcctg      360

agggagctgg aggccaatgc cagaagggct ggttgcacaa gaggctgcct catttgcctc      420

tcccacatta agtgcacccc taagatgaag aaatttatcc ctggcaggtg ccacacttat      480

gaaggtgaaa aggagtctgc tcagggaggg attggagagg caattgttga tatcccagag      540

attcctggct tcaaggataa ggagccactg gaccagttta ttgctcaagt ggacctctgt      600

gctgattgca ccactggctg tctgaagggc cttgccaatg tccagtgctc tgacctcctg      660

aagaagtggc ttccccagag gtgtaccact tttgccagca agattcaggg tagggtggac      720

aaaatcaagg gtctggctgg ggacagatga ttagctagct ggccagacat gataagatac      780

attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa      840

atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac      900

aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc      960

aagtaaaacc tctacaaatg tggtatggaa aaaaaaaa                              998


<210>  2
<211>  1087
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2
atttaggtga cactatagaa tgaccctgct tgctcaactc tacgtctttg tttcgttttc       60

tgttctgcgc cgttacagat ccaagctgtg accggcgcct acctgagatc accggtcacc      120

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      180

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      240

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      300

ctcgtgacca ccctgaccta cggcgtgcag gcttcagccg ctaccccgac cacatgaagc      360

agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct      420

tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg      480

tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca      540

agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg      600

gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg      660

accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact      720

acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc      780

tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat      840

tagctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa      900

tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca      960

ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc     1020

agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggaaa     1080

aaaaaaa                                                               1087


<210>  3
<211>  829
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3
atttaggtga cactatagaa tgaccctgct tgctcaactc tacgtctttg tttcgttttc       60

tgttctgcgc cgttacagat ccaagctgtg accggcgcct acctgagatc accggtcacc      120

atgggtctca cctcccaact gcttccccct ctgttcttcc tgctagcatg tgccggcaac      180

tttgtccacg gacacaagtg cgatatcacc ttacaggaga tcatcaaaac tttgaacagc      240

ctcacagagc agaagactct gtgcaccgag ttgaccgtaa cagacatctt tgctgcctcc      300

aagaacacaa ctgagaagga aaccttctgc agggctgcga ctgtgctccg gcagttctac      360

agccaccatg agaaggacac tcgctgcctg ggtgcgactg cacagcagtt ccacaggcac      420

aagcagctga tccgattcct gaaacggctc gacaggaacc tctggggcct ggcgggcttg      480

aattcctgtc ctgtgaagga agccaaccag agtacgttgg aaaacttctt ggaaaggcta      540

aagacgatca tgagagagaa atattcaaag tgttcgagct gatagctagc tggccagaca      600

tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct      660

ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac      720

aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg      780

ttttttaaag caagtaaaac ctctacaaat gtggtatgga aaaaaaaaa                  829


<210>  4
<211>  1129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4
atttaggtga cactatagaa tgaccctgct tgctcaactc tacgtctttg tttcgttttc       60

tgttctgcgc cgttacagat ccaagctgtg accggcgcct acctgagatc accggtcacc      120

atgtggcccc ctgggtcagc ctcccagcca ccgccctcac ctgccgcggc cacaggtctg      180

catccagcgg ctcgccctgt gtccctgcag tgccggctca gcatgtgtcc agcgcgcagc      240

ctcctccttg tggctaccct ggtcctcctg gaccacctca gtttggccag aaacctcccc      300

gtggccactc cagacccagg aatgttccca tgccttcacc actcccaaaa cctgctgagg      360

gccgtcagca acatgctcca gaaggccaga caaactctag aattttaccc ttgcacttct      420

gaagagattg atcatgaaga tatcacaaaa gataaaacca gcacagtgga ggcctgttta      480

ccattggaat taaccaagaa tgagagttgc ctaaattcca gagagacctc tttcataact      540

aatgggagtt gcctggcctc cagaaagacc tcttttatga tggccctgtg ccttagtagt      600

atttatgaag acttgaagat gtaccaggtg gagttcaaga ccatgaatgc aaagcttctg      660

atggatccta agaggcagat ctttctagat caaaacatgc tggcagttat tgatgagctg      720

atgcaggccc tgaatttcaa cagtgagact gtgccacaaa aatcctccct tgaagaaccg      780

gatttttata aaactaaaat caagctctgc atacttcttc atgctttcag aattcgggca      840

gtgactattg atagagtgat gagctatctg aatgcttcct aatagctagc tggccagaca      900

tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct      960

ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac     1020

aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg     1080

ttttttaaag caagtaaaac ctctacaaat gtggtatgga aaaaaaaaa                 1129


<210>  5
<211>  1354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5
atttaggtga cactatagaa tgaccctgct tgctcaactc tacgtctttg tttcgttttc       60

tgttctgcgc cgttacagat ccaagctgtg accggcgcct acctgagatc accggtcacc      120

atgtgtcacc agcagttggt catctcttgg ttttccctgg tttttctggc atctcccctc      180

gtggccatat gggaactgaa gaaagatgtt tatgtcgtag aattggattg gtatccggat      240

gcccctggag aaatggtggt cctcacctgt gacacccctg aagaagatgg tatcacctgg      300

accttggacc agagcagtga ggtcttaggc tctggcaaaa ccctgaccat ccaagtcaaa      360

gagtttggag atgctggcca gtacacctgt cacaaaggag gcgaggttct aagccattcg      420

ctcctgctgc ttcacaaaaa ggaagatgga atttggtcca ctgatatttt aaaggaccag      480

aaagaaccca aaaataagac ctttctaaga tgcgaggcca agaattattc tggacgtttc      540

acctgctggt ggctgacgac aatcagtact gatttgacat tcagtgtcaa aagcagcaga      600

ggctcttctg acccccaagg ggtgacgtgc ggagctgcta cactctctgc agagagagtc      660

agaggggaca acaaggagta tgagtactca gtggagtgcc aggaggacag tgcctgccca      720

gctgctgagg agagtctgcc cattgaggtc atggtggatg ccgttcacaa gctcaagtat      780

gaaaactaca ccagcagctt cttcatcagg gacatcatca aacctgaccc acccaagaac      840

ttgcagctga agccattaaa gaattctcgg caggtggagg tcagctggga gtaccctgac      900

acctggagta ctccacattc ctacttctcc ctgacattct gcgttcaggt ccagggcaag      960

agcaagagag aaaagaaaga tagagtcttc acggacaaga cctcagccac ggtcatctgc     1020

cgcaaaaatg ccagcattag cgtgcgggcc caggaccgct actatagctc atcttggagc     1080

gaatgggcat ctgtgccctg cagttagtag ctagctggcc agacatgata agatacattg     1140

atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt     1200

gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca     1260

attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt     1320

aaaacctcta caaatgtggt atggaaaaaa aaaa                                 1354


<210>  6
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  R can be A or G

<400>  6
gccrccatgg                                                              10


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7
ccggtcacca tg                                                           12


<210>  8
<211>  23
<212>  DNA
<213>  Bacteriophage T7

<400>  8
taatacgact cactataggg aga                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Bacteriophage T3

<400>  9
aattaaccct cactaaaggg aga                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Bacteriophage K11

<400>  10
aattagggca cactataggg aga                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Bacteriophage SP6

<400>  11
atttacgaca cactatagaa gaa                                               23


<210>  12
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  N can be any nucleoside base

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  R can by any purine base (adenosine or guanosine)

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  N can be any nucleoside base

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  R can by any purine base (adenosine or guanosine)

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  N can be any nucleoside base

<400>  12
nnnnnnrnca tgrcnn                                                       16


<210>  13
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  N can be any nucleoside base

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  N can be any nucleoside base

<400>  13
nnngncacca tggcgg                                                       16


