               SEQUENCE LISTING

<110> GENIE BIOTECH UK LTD

<120> Synthesis of lactone derivatives and their use in the modification of proteins

<130> 228 738

<160> 93

<170> BiSSAP 1.3.6

<210> 1
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 1
Met Gly Ser Ser His His His His His His 
1               5                   10  

<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 2
Gly His His His His His His 
1               5           

<210> 3
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 3
Gly Ser Ser His His His His His His 
1               5                   

<210> 4
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> An amino acid residue or absent

<220> 
<221> VARIANT
<222> 3
<223> An amino acid residue or absent

<220> 
<221> VARIANT
<222> 4
<223> 3 or more His residues

<400> 4
Gly Xaa Xaa Xaa 
1               

<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Gly, Ser or Ala

<400> 5
Gly Xaa Thr Tyr Ser Asp His 
1               5           

<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Gly, Ser or Ala

<400> 6
Gly Xaa Thr Tyr Ser Ala His 
1               5           

<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Gly, Ser or Ala

<400> 7
Gly Xaa Thr Tyr Ser Cys His 
1               5           

<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Gly, Ser or Ala

<400> 8
Gly Xaa Lys Trp Ser Lys Arg 
1               5           

<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Gly, Ser or Ala

<400> 9
Gly Xaa Ser Gly Ser Lys 
1               5       

<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 10
Thr Tyr Ser Asp His 
1               5   

<210> 11
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 11
Thr Tyr Ser Ala His 
1               5   

<210> 12
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 12
Thr Tyr Ser Cys His 
1               5   

<210> 13
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 13
Lys Trp Ser Lys Arg 
1               5   

<210> 14
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 14
Ser Gly Ser Lys 
1               

<210> 15
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 15
Gly Gly Thr Tyr Ser Asp His 
1               5           

<210> 16
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 16
Gly Gly Thr Tyr Ser Cys His 
1               5           

<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 17
Gly Gly Lys Trp Ser Lys Arg 
1               5           

<210> 18
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 18
Gly Ala Ser Gly Ser Lys 
1               5       

<210> 19
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 1
<223> Ala, Gly, Ser, His or Leu

<220> 
<221> VARIANT
<222> 2
<223> An amino acid residue or absent

<220> 
<221> VARIANT
<222> 3
<223> An amino acid residue or absent

<220> 
<221> VARIANT
<222> 4
<223> 3 or more His residues

<400> 19
Xaa Xaa Xaa Xaa 
1               

<210> 20
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 20
Ala His His His 
1               

<210> 21
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 21
Gly Ala Ala His 
1               

<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 22
Gly Ala Ser His 
1               

<210> 23
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 23
Gly His His His 
1               

<210> 24
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 24
Gly Ser Ala His 
1               

<210> 25
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 25
Gly Ser Ser His 
1               

<210> 26
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 26
Ser Ser Tyr His 
1               

<210> 27
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 27
Ser Tyr Tyr His 
1               

<210> 28
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 28
Val His His His 
1               

<210> 29
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 29
Gly Ala Pro Thr Leu 
1               5   

<210> 30
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 30
Gly His His His His 
1               5   

<210> 31
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 31
Gly His His His His His 
1               5       

<210> 32
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 32
Leu Arg Phe Lys Phe Tyr 
1               5       

<210> 33
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 33
His His His His His His 
1               5       

<210> 34
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 34
Gly Ala Ser Gly Ser Lys Gly 
1               5           

<210> 35
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 35
Gly Leu Arg Phe Lys Phe Tyr 
1               5           

<210> 36
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 36
His Leu Arg Phe Lys Phe Tyr 
1               5           

<210> 37
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 37
Lys His His His His His His 
1               5           

<210> 38
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 38
Gly His Leu Arg Phe Lys Phe Tyr 
1               5               

<210> 39
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 39
Gly Ser Leu Arg Phe Lys Phe Tyr 
1               5               

<210> 40
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 40
Gly Ser His His His His His His 
1               5               

<210> 41
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 41
Gly Ser His Leu Arg Phe Lys Phe Tyr 
1               5                   

<210> 42
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 42
Arg Gly Ser His His His His His His 
1               5                   

<210> 43
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 43
Ser Tyr Tyr His His His His His His 
1               5                   

<210> 44
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 44
Gly His Leu Arg 
1               

<210> 45
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 45
Gly Leu Arg Phe 
1               

<210> 46
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 46
Gly Ala Pro Thr 
1               

<210> 47
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 47
Gly Ala Ser Gly 
1               

<210> 48
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 48
Gly Gly Lys Trp 
1               

<210> 49
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 49
Gly Gly Thr Tyr 
1               

<210> 50
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 50
Gly Ser His Leu 
1               

<210> 51
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 51
Gly Ser Leu Arg 
1               

<210> 52
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 52
Gly Ala Ser Gly Ser 
1               5   

<210> 53
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 53
Gly Gly Lys Trp Ser 
1               5   

<210> 54
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 54
Gly Gly Thr Tyr Ser 
1               5   

<210> 55
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 55
Gly His Leu Arg Phe 
1               5   

<210> 56
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 56
Gly Leu Arg Phe Lys 
1               5   

<210> 57
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 57
Gly Ser His Leu Arg 
1               5   

<210> 58
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 58
Gly Ser Leu Arg Phe 
1               5   

<210> 59
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 59
Gly Ser Ser His His 
1               5   

<210> 60
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 60
Gly Ala Ser Gly Ser Lys 
1               5       

<210> 61
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 61
Gly Gly Lys Trp Ser Lys 
1               5       

<210> 62
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 62
Gly Gly Thr Tyr Ser Asp 
1               5       

<210> 63
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 63
Gly His Leu Arg Phe Lys 
1               5       

<210> 64
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 64
Gly Leu Arg Phe Lys Phe 
1               5       

<210> 65
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 65
Gly Ser His Leu Arg Phe 
1               5       

<210> 66
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 66
Gly Ser Leu Arg Phe Lys 
1               5       

<210> 67
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 67
Gly Ser Ser His His His 
1               5       

<210> 68
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 68
Gly His Leu Arg Phe Lys Phe 
1               5           

<210> 69
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 69
Gly Ser His Leu Arg Phe Lys 
1               5           

<210> 70
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 70
Gly Ser Leu Arg Phe Lys Phe 
1               5           

<210> 71
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 71
Gly Ser Ser His His His His 
1               5           

<210> 72
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 72
Gly Ser His Leu Arg Phe Lys Phe 
1               5               

<210> 73
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 73
Gly Ser Ser His His His His His 
1               5               

<210> 74
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 74
His Leu Arg Phe 
1               

<210> 75
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 75
His Leu Arg Phe Lys 
1               5   

<210> 76
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 76
His Leu Arg Phe Lys Phe 
1               5       

<210> 77
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 77
Leu Arg Phe Lys 
1               

<210> 78
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 78
Leu Arg Phe Lys Phe 
1               5   

<210> 79
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 2
<223> Ser, Gly or Ala

<220> 
<221> VARIANT
<222> 3
<223> Ser, Gly or Ala

<220> 
<221> VARIANT
<222> 4
<223> 3 or more His residues

<400> 79
Gly Xaa Xaa Xaa 
1               

<210> 80
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<220> 
<221> VARIANT
<222> 4
<223> 3 or more His residues

<400> 80
Gly Ser Ser Xaa 
1               

<210> 81
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 81
Gly Arg Gly Asp Ser Pro Cys 
1               5           

<210> 82
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 82
Ala His His His His His His 
1               5           

<210> 83
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 83
Gly His His His His His His His His His His 
1               5                   10      

<210> 84
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 84
Gly His His His His His His His His 
1               5                   

<210> 85
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 85
Gly Gly Ser His His His His His His 
1               5                   

<210> 86
<211> 133
<212> PRT
<213> Artificial Sequence


<220> 
<223> Qbeta

<400> 86
Met Ala Lys Leu Glu Thr Val Thr Leu Gly Asn Ile Gly Lys Asp Gly 
1               5                   10                  15      
Lys Gln Thr Leu Val Leu Asn Pro Arg Gly Val Asn Pro Thr Asn Gly 
            20                  25                  30          
Val Ala Ser Leu Ser Gln Ala Gly Ala Val Pro Ala Leu Glu Lys Arg 
        35                  40                  45              
Val Thr Val Ser Val Ser Gln Pro Ser Arg Asn Arg Lys Asn Tyr Lys 
    50                  55                  60                  
Val Gln Val Lys Ile Gln Asn Pro Thr Ala Cys Thr Ala Asn Gly Ser 
65                  70                  75                  80  
Cys Asp Pro Ser Val Thr Arg Gln Ala Tyr Ala Asp Val Thr Phe Ser 
                85                  90                  95      
Phe Thr Gln Tyr Ser Thr Asp Glu Glu Arg Ala Phe Val Arg Thr Glu 
            100                 105                 110         
Leu Ala Ala Leu Leu Ala Ser Pro Leu Leu Ile Asp Ala Ile Asp Gln 
        115                 120                 125             
Leu Asn Pro Ala Tyr 
    130             

<210> 87
<211> 201
<212> PRT
<213> Artificial Sequence


<220> 
<223> His-RBD

<400> 87
Gly His His His His His His Thr Asn Leu Cys Pro Phe Gly Glu Val 
1               5                   10                  15      
Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg 
            20                  25                  30          
Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser 
        35                  40                  45              
Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp 
    50                  55                  60                  
Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp 
65                  70                  75                  80  
Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr 
                85                  90                  95      
Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn 
            100                 105                 110         
Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr 
        115                 120                 125             
Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser 
    130                 135                 140                 
Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly 
145                 150                 155                 160 
Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn 
                165                 170                 175     
Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu 
            180                 185                 190         
Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200     

<210> 88
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 88
gaccccaaaa tcagcgaaat                                                20


<210> 89
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 89
tctggttact gccagttgaa tctg                                           24


<210> 90
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Probe

<400> 90
accccgcatt acgtttggtg gacc                                           24


<210> 91
<211> 194
<212> PRT
<213> Artificial Sequence


<220> 
<223> RBD

<400> 91
Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
1               5                   10                  15      
Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            20                  25                  30          
Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        35                  40                  45              
Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    50                  55                  60                  
Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
65                  70                  75                  80  
Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                85                  90                  95      
Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            100                 105                 110         
Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        115                 120                 125             
Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    130                 135                 140                 
Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
145                 150                 155                 160 
Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
                165                 170                 175     
Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            180                 185                 190         
Cys Gly 
        

<210> 92
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> His-tagged Beltide-1 derivative peptide PP6

<400> 92
Gly Ser Ser His His His His His His Asp Trp Leu Lys Ala Phe Tyr 
1               5                   10                  15      
Asp Lys Val Ala Glu Lys Leu Lys Glu Ala Phe 
            20                  25          

<210> 93
<211> 29
<212> PRT
<213> Artificial Sequence


<220> 
<223> His-tagged peptide

<400> 93
Gly Ser Ser His His His His His His Gly Gly Thr Tyr Ser Ala His 
1               5                   10                  15      
Phe Gly Pro Leu Thr Trp Val Ala Lys Pro Gln Gly Gly 
            20                  25                  

