                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Curtis, Erik M.
       Konstantinidis, Spyridon
       Poplyk, Murphy
       Swartz, Andrew Ryan
       Wenger, Marc D.
 
<120>  PURIFIED COMPOSITIONS OF ENTEROVIRUSES AND METHODS OF PURIFICATION 
       WITH GLUTATHIONE AFFINITY CHROMATOGRAPHY

<130>  24943-WO-PCT

<150>  US 62/951,078
<151>  2019-12-20

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  7405
<212>  RNA
<213>  Coxsackievirus A 21

<400>  1
uuaaaacagc ucugggguug uucccacccc agaggcccac guggcggcua guacucuggu       60

auuacgguac cuuuguacgc cuguuuugua ucccuucccc cguaacuuua gaagcuuauc      120

aaagguucaa uagcaggggu acaaaccagu accucuacga acaagcacuu cuguuucccc      180

ggugauauca cauagacugu acccacgguc aaaagugauu gauccguuau ccgcuugagu      240

acuucgagaa gccuaguauc accuuggaau cuucgaugcg uugcgcucaa cacucugccc      300

cgaguguagc uuaggcugau gagucugggc acuccccacc ggcgacggug gcccaggcug      360

cguuggcggc cuacccaugg cugaugccgu gggacgcuag uugugaacaa ggugugaaga      420

gccuauugag cuacucaaga guccuccggc cccugaaugc ggcuaauccu aaccacggag      480

caaccgcuca caacccagug aguagguugu cguaaugcgu aagucugugg cggaaccgac      540

uacuuugggu guccguguuu cccuuuauau ucauacuggc ugcuuauggu gacaauuuac      600

aaauuguuac cauauagcua uuggauuggc cacccaguau ugugcaauau auuugagugu      660

uucuuucaua agccuuauua acaucacauu uuuaaucaca auaaacagug caaauggggg      720

cucaaguuuc aacgcaaaag accggugcgc acgagaauca aaacguggca gccaauggau      780

ccaccauuaa uuacacuacu aucaacuauu acaaagacag ugcgaguaau uccgcuacua      840

gacaagaccu cucccaagau ccaucaaaau ucacagaacc gguuaaggac uuaauguuga      900

aaacagcacc agcucuaaac ucgccuaacg uggaagcaug uggguacagu gaccguguga      960

ggcaaaucac uuuaggcaac ucgacuauua cuacacaaga agcagccaau gcuauuguug     1020

cuuacgguga auggcccacu uacauaaaug auucagaagc uaauccggua gaugcaccca     1080

cugagccaga uguuaguagc aaccgguuuu acacccuaga aucggugucu uggaagacca     1140

cuucaagggg augguggugg aaguuaccag auuguuugaa ggacauggga auguuugguc     1200

agaauaugua cuaucacuac uuggggcgcu cugguuacac cauucauguc cagugcaacg     1260

cuucaaaauu ucaccaaggg gcguuaggag uuuuucugau accagaguuu gucauggcuu     1320

gcaacacuga gaguaaaacg ucauacguuu cauacaucaa ugcaaauccu ggugagagag     1380

gcggugaguu uacgaacacc uacaauccgu uaaauacaga cgccagugag ggcagaaagu     1440

uugcagcauu ggauuauuug cuggguucug guguucuagc aggaaacgcc uuuguguacc     1500

cgcaccagau caucaaccua cguaccaaca acagugcaac aauuguggug ccauacguaa     1560

acucacuugu gauugauugu auggcaaaac acaauaacug gggcauuguc auauuaccac     1620

uggcacccuu ggccuuugcc gcaacaucgu caccacaggu gccuauuaca gugaccauug     1680

cacccaugug uacagaauuc aauggguuga gaaacaucac cgucccagua caucaagggu     1740

ugccgacaau gaacacaccu gguuccaauc aauuccuuac aucugaugac uuccagucgc     1800

ccugugccuu accuaauuuu gauguuacuc caccaauaca cauacccggg gaaguaaaga     1860

auaugaugga acuagcugaa auugacacau ugaucccaau gaacgcagug gacgggaagg     1920

ugaacacaau ggagauguau caaauaccau ugaaugacaa uuugagcaag gcaccuauau     1980

ucuguuuauc ccuaucaccu gcuucugaua aacgacugag ccgcaccaug uugggugaaa     2040

uccuaaauua uuacacccau uggacggggu ccaucagguu caccuuucua uuuuguggua     2100

guaugauggc cacugguaaa cugcuccuca gcuauucccc accgggagcu aaaccaccaa     2160

ccaaucgcaa ggaugcaaug cuaggcacac acaucaucug ggaccuaggg uuacaaucca     2220

guuguuccau gguugcaccg uggaucucca acacagugua cagacggugu gcacgugaug     2280

acuucacuga gggcggauuu auaacuugcu ucuaucaaac uagaauugug guaccugcuu     2340

caaccccuac caguauguuc auguuaggcu uuguuagugc guguccagac uucaguguca     2400

gacugcuuag ggacacuccc cauauuaguc aaucgaaacu aauaggacgu acacaaggca     2460

uugaagaccu cauugacaca gcgauaaaga augccuuaag agugucccaa ccacccucga     2520

cccagucaac ugaagcaacu aguggaguga auagccagga ggugccagcu cuaacugcug     2580

uggaaacagg agcaucuggu caagcaaucc ccagugaugu gguggaaacu aggcacgugg     2640

uaaauuacaa aaccaggucu gaaucguguc uugagucauu cuuugggaga gcugcgugug     2700

ucacaauccu auccuugacc aacuccucca agagcggaga ggagaaaaag cauuucaaca     2760

uauggaauau uacauacacc gacacugucc aguuacgcag aaaauuagag uuuuucacgu     2820

auuccagguu ugaucuugaa augacuuuug uauucacaga gaacuauccu aguacagcca     2880

guggagaagu gcgaaaccag guguaccaga ucauguauau uccaccaggg gcaccccgcc     2940

caucauccug ggaugacuac acauggcaau ccucuucaaa cccuuccauc uucuacaugu     3000

auggaaaugc accuccacgg augucaauuc cuuacguagg gauugccaau gccuauucac     3060

acuucuacga uggcuuugca cgggugccac uugaggguga gaacaccgau gcuggcgaca     3120

cguuuuacgg uuuagugucc auaaaugauu uuggaguuuu agcaguuaga gcaguaaacc     3180

gcaguaaucc acauacaaua cacacaucug ugagagugua caugaaacca aaacacauuc     3240

gguguuggug ccccagaccu ccucgagcug uauuauacag gggagaggga guggacauga     3300

uauccagugc aauucuaccu cugaccaagg uagacucaau uaccacuuuu ggguuugguc     3360

aucagaacaa agcaguguac guugccgguu acaagauuug caacuaccac cuagcaaccc     3420

caagugauca cuugaaugca auuaguaugu uaugggacag ggauuuaaug gugguggaau     3480

cuagagccca gggaacugau accaucgcca gauguaguug caggugugga guuuacuauu     3540

gugaaucuag gaggaaguac uacccuguca cuuuuacugg cccaacguuu cgauucaugg     3600

aagcaaacga cuacuaucca gcaagauacc agucucacau gcugauaggg ugcggauuug     3660

cagaacccgg ggacugcggu gggauacuga ggugcacuca ugggguaauu gguaucauua     3720

cugcaggagg ugaaggggua guagccuuug cugacauuag agaccucugg guguaugaag     3780

aggaggccau ggaacaggga auaacaagcu acaucgaauc ucucggcaca gccuuuggcg     3840

caggguucac ccacacaauc agugagaaag ugacugaauu gacaacaaug guuaccagca     3900

cuaucacaga aaaacuacug aaaaacuugg ugaaaauagu gucggcucua gugauuguug     3960

ugagaaauua ugaggacacu accacgaucc uugcaacacu agcacuacuc gggugugaua     4020

uaucuccuug gcaaugguug aagaagaagg caugugacuu acuagagauu ccuuauguga     4080

ugcgccaagg ugaugggugg augaagaaau ucacagaggc gugcaaugca gcuaaaggcu     4140

uagaguggau uagcaacaaa auuuccaagu uuauagauug guugaagugu aaaauuaucc     4200

cagacgcuaa ggacaaggug gaauuucuca ccaaguugaa acagcuagac auguuggaaa     4260

aucaaauugc aaccauccac caaucuugcc ccagccaaga acaacaagag auucuuuuca     4320

acaaugugag auggcuagca guccaguccc gucgguuugc accauuauac gcuguggagg     4380

cacgccgaau uaacaaaaug gagagcacaa uaaacaauua uauacaguuc aagagcaaac     4440

accguauuga accaguaugu augcucauuc augggucacc agggacgggu aaaucuauag     4500

cuacuucauu aauagguaga gcaauagcag agaaggaaag cacaucaguc uauucaaugc     4560

caccugaccc aucucacuuu gauggcuaua aacaacaagg gguagugauu auggacgacc     4620

uaaaccaaaa ccccgauggu auggacauga aacuguuuug ccaaauggua ucaacagugg     4680

aguuuauucc uccaauggcc ucauuagagg agaagggcau uuuguuuaca ucugauuaug     4740

uccuggcuuc uaccaacucu cauucaauug uaccacccac aguggcucac agugaugccu     4800

uaaccagacg auuugcauuu gauguggagg uuuacacgau gucugaacau ucagucaaag     4860

gcaaacugaa uauggccacg gccacucaau uguguaagga uuguccaaca ccugcaaauu     4920

uuaaaaagug uugcccucuc guuuguggaa aggccuugca auuaauggac agguacacca     4980

gacaaagguu cacuguagau gagauuacca cauuaaucau gaaugagaaa aacagaaggg     5040

ccaauaucgg caauugcaug gaagccuugu uucaaggacc auuaagguau aaagauuuga     5100

agaucgaugu gaagacaguu ccccccccug agugcaucag ugauuuguua caagcagugg     5160

auucucaaga gguuagggau uacugugaga agaaaggcug gaucguuaac guuacuagcc     5220

agauucaacu agaaaggaac aucaauaggg ccaugacuau acuccaagcu guuaccacau     5280

ucgcagcagu cgcaggagua guguauguaa uguacaaacu cuucgccggu caacagggug     5340

cauacacugg cuugccaaac aaaaaaccca augucccuac uaucagaguc gcuaaagucc     5400

aggggccagg auuugacuac gcaguggcaa uggcaaaaag aaacauaguu acugcaacca     5460

ccaccaaggg ugaauuuacc augcuagggg ugcaugauaa uguagcaaua uugccaaccc     5520

augccgcucc aggagaaacc auuauuauug augggaaaga aguagagauc cuagaugcca     5580

gagccuuaga agaucaagcg ggaaccaauc uugagaucac cauuauuacu cuaaaaagaa     5640

augagaaguu uagagacauc agaucacaua uucccaccca aauuacugaa acuaacgaug     5700

gaguguugau cgugaacacu agcaaguacc ccaauaugua uguccccguu ggugcuguga     5760

ccgaacaggg auaucuuaau cucaguggac gucaaacugc ucgcacuuua auguacaacu     5820

uuccaacaag ggcaggccag ugcggaggaa ucaucacuug uacuggcaaa gucauuggga     5880

ugcauguugg cgggaacggu ucacaugggu uugcagcagc ccucaagcga ucauacuuca     5940

cucaaaauca gggcgaaauc caguggauga ggucaucaaa agaagugggg uaccccauua     6000

uaaaugcccc auccaagaca aaguuagaac ccagugcuuu ccacuauguu uuugaaggug     6060

uuaaggaacc agcuguacuc acuaagaaug accccagacu aaaaacagau uuugaagaag     6120

ccaucuuuuc uaaauaugug gggaacaaaa uuacugaagu ggacgaguac augaaagaag     6180

caguggauca cuaugcagga caguuaaugu cacuggauau caacacagaa cagaugugcc     6240

uggaggaugc cauguacggc accgaugguc uugaggcccu ggaucuuagc acuagugcug     6300

gauauccuua uguugcaaug gggaaaaaga aaagagacau ucuaaauaaa cagaccagag     6360

auacuaagga gaugcagaga cuuuuagaua ccuauggaau caaucuacca uuagucacgu     6420

acgugaaaga ugaacucagg ucaaagacua aaguggaaca aggaaaguca agauugauug     6480

aagcuuccag ccuuaaugau ucaguugcaa ugagaauggc cuuuggcaau cuuuacgcag     6540

cuuuccacaa gaauccaggu guggugacag gaucagcagu ugguugugac ccagauuugu     6600

uuuggaguaa gauaccagug cuaauggaag aaaaacucuu cgcuuuugac uacacagggu     6660

augaugccuc acucagcccu gcuugguuug aagcucuuaa aaugguguua gaaaaaauug     6720

gauuuggcag uagaguagac uauauagacu accugaacca cucucaccac cuuuacaaaa     6780

acaagacuua uugugucaaa ggcggcaugc cauccggcug cucuggcacc ucaauuuuca     6840

acucaaugau uaacaaccug aucauuagga cgcuuuuacu gagaaccuac aagggcauag     6900

acuuggacca uuuaaaaaug auugccuaug gugaugacgu gauagcuucc uacccccaug     6960

agguugacgc uagucuccua gcccaaucag gaaaagacua uggacuaacc augacuccag     7020

cagauaaauc aguaaccuuu gaaacaguca caugggagaa uguaacauuu cugaaaagau     7080

uuuucagagc agaugagaag uauccauucc uggugcaucc agugaugcca augaaagaaa     7140

uucacgaauc aaucagaugg accaaggacc cuagaaacac acaggaucac guacgcucgu     7200

ugugccuauu agcuuggcac aacggugaag aagaauacaa uaaauuuuua gcuaaaauca     7260

gaagugugcc aauuggaaga gcuuuauugc ucccagagua cucuacauug uaccgccgau     7320

ggcucgacuc auuuuaguaa cccuaccuca gucggauugg auuggguuac acuguuguag     7380

ggguaaauuu uucuuuaauu cggag                                           7405


<210>  2
<211>  298
<212>  PRT
<213>  Coxsackievirus A 21

<400>  2

Gly Ile Glu Asp Leu Ile Asp Thr Ala Ile Lys Asn Ala Leu Arg Val 
1               5                   10                  15      


Ser Gln Pro Pro Ser Thr Gln Ser Thr Glu Ala Thr Ser Gly Val Asn 
            20                  25                  30          


Ser Gln Glu Val Pro Ala Leu Thr Ala Val Glu Thr Gly Ala Ser Gly 
        35                  40                  45              


Gln Ala Ile Pro Ser Asp Val Val Glu Thr Arg His Val Val Asn Tyr 
    50                  55                  60                  


Lys Thr Arg Ser Glu Ser Cys Leu Glu Ser Phe Phe Gly Arg Ala Ala 
65                  70                  75                  80  


Cys Val Thr Ile Leu Ser Leu Thr Asn Ser Ser Lys Ser Gly Glu Glu 
                85                  90                  95      


Lys Lys His Phe Asn Ile Trp Asn Ile Thr Tyr Thr Asp Thr Val Gln 
            100                 105                 110         


Leu Arg Arg Lys Leu Glu Phe Phe Thr Tyr Ser Arg Phe Asp Leu Glu 
        115                 120                 125             


Met Thr Phe Val Phe Thr Glu Asn Tyr Pro Ser Thr Ala Ser Gly Glu 
    130                 135                 140                 


Val Arg Asn Gln Val Tyr Gln Ile Met Tyr Ile Pro Pro Gly Ala Pro 
145                 150                 155                 160 


Arg Pro Ser Ser Trp Asp Asp Tyr Thr Trp Gln Ser Ser Ser Asn Pro 
                165                 170                 175     


Ser Ile Phe Tyr Met Tyr Gly Asn Ala Pro Pro Arg Met Ser Ile Pro 
            180                 185                 190         


Tyr Val Gly Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Phe Ala 
        195                 200                 205             


Arg Val Pro Leu Glu Gly Glu Asn Thr Asp Ala Gly Asp Thr Phe Tyr 
    210                 215                 220                 


Gly Leu Val Ser Ile Asn Asp Phe Gly Val Leu Ala Val Arg Ala Val 
225                 230                 235                 240 


Asn Arg Ser Asn Pro His Thr Ile His Thr Ser Val Arg Val Tyr Met 
                245                 250                 255     


Lys Pro Lys His Ile Arg Cys Trp Cys Pro Arg Pro Pro Arg Ala Val 
            260                 265                 270         


Leu Tyr Arg Gly Glu Gly Val Asp Met Ile Ser Ser Ala Ile Leu Pro 
        275                 280                 285             


Leu Thr Lys Val Asp Ser Ile Thr Thr Phe 
    290                 295             


<210>  3
<211>  272
<212>  PRT
<213>  Coxsackievirus A 21

<400>  3

Ser Pro Asn Val Glu Ala Cys Gly Tyr Ser Asp Arg Val Arg Gln Ile 
1               5                   10                  15      


Thr Leu Gly Asn Ser Thr Ile Thr Thr Gln Glu Ala Ala Asn Ala Ile 
            20                  25                  30          


Val Ala Tyr Gly Glu Trp Pro Thr Tyr Ile Asn Asp Ser Glu Ala Asn 
        35                  40                  45              


Pro Val Asp Ala Pro Thr Glu Pro Asp Val Ser Ser Asn Arg Phe Tyr 
    50                  55                  60                  


Thr Leu Glu Ser Val Ser Trp Lys Thr Thr Ser Arg Gly Trp Trp Trp 
65                  70                  75                  80  


Lys Leu Pro Asp Cys Leu Lys Asp Met Gly Met Phe Gly Gln Asn Met 
                85                  90                  95      


Tyr Tyr His Tyr Leu Gly Arg Ser Gly Tyr Thr Ile His Val Gln Cys 
            100                 105                 110         


Asn Ala Ser Lys Phe His Gln Gly Ala Leu Gly Val Phe Leu Ile Pro 
        115                 120                 125             


Glu Phe Val Met Ala Cys Asn Thr Glu Ser Lys Thr Ser Tyr Val Ser 
    130                 135                 140                 


Tyr Ile Asn Ala Asn Pro Gly Glu Arg Gly Gly Glu Phe Thr Asn Thr 
145                 150                 155                 160 


Tyr Asn Pro Leu Asn Thr Asp Ala Ser Glu Gly Arg Lys Phe Ala Ala 
                165                 170                 175     


Leu Asp Tyr Leu Leu Gly Ser Gly Val Leu Ala Gly Asn Ala Phe Val 
            180                 185                 190         


Tyr Pro His Gln Ile Ile Asn Leu Arg Thr Asn Asn Ser Ala Thr Ile 
        195                 200                 205             


Val Val Pro Tyr Val Asn Ser Leu Val Ile Asp Cys Met Ala Lys His 
    210                 215                 220                 


Asn Asn Trp Gly Ile Val Ile Leu Pro Leu Ala Pro Leu Ala Phe Ala 
225                 230                 235                 240 


Ala Thr Ser Ser Pro Gln Val Pro Ile Thr Val Thr Ile Ala Pro Met 
                245                 250                 255     


Cys Thr Glu Phe Asn Gly Leu Arg Asn Ile Thr Val Pro Val His Gln 
            260                 265                 270         


<210>  4
<211>  240
<212>  PRT
<213>  Coxsackievirus A 21

<400>  4

Gly Leu Pro Thr Met Asn Thr Pro Gly Ser Asn Gln Phe Leu Thr Ser 
1               5                   10                  15      


Asp Asp Phe Gln Ser Pro Cys Ala Leu Pro Asn Phe Asp Val Thr Pro 
            20                  25                  30          


Pro Ile His Ile Pro Gly Glu Val Lys Asn Met Met Glu Leu Ala Glu 
        35                  40                  45              


Ile Asp Thr Leu Ile Pro Met Asn Ala Val Asp Gly Lys Val Asn Thr 
    50                  55                  60                  


Met Glu Met Tyr Gln Ile Pro Leu Asn Asp Asn Leu Ser Lys Ala Pro 
65                  70                  75                  80  


Ile Phe Cys Leu Ser Leu Ser Pro Ala Ser Asp Lys Arg Leu Ser Arg 
                85                  90                  95      


Thr Met Leu Gly Glu Ile Leu Asn Tyr Tyr Thr His Trp Thr Gly Ser 
            100                 105                 110         


Ile Arg Phe Thr Phe Leu Phe Cys Gly Ser Met Met Ala Thr Gly Lys 
        115                 120                 125             


Leu Leu Leu Ser Tyr Ser Pro Pro Gly Ala Lys Pro Pro Thr Asn Arg 
    130                 135                 140                 


Lys Asp Ala Met Leu Gly Thr His Ile Ile Trp Asp Leu Gly Leu Gln 
145                 150                 155                 160 


Ser Ser Cys Ser Met Val Ala Pro Trp Ile Ser Asn Thr Val Tyr Arg 
                165                 170                 175     


Arg Cys Ala Arg Asp Asp Phe Thr Glu Gly Gly Phe Ile Thr Cys Phe 
            180                 185                 190         


Tyr Gln Thr Arg Ile Val Val Pro Ala Ser Thr Pro Thr Ser Met Phe 
        195                 200                 205             


Met Leu Gly Phe Val Ser Ala Cys Pro Asp Phe Ser Val Arg Leu Leu 
    210                 215                 220                 


Arg Asp Thr Pro His Ile Ser Gln Ser Lys Leu Ile Gly Arg Thr Gln 
225                 230                 235                 240 


<210>  5
<211>  69
<212>  PRT
<213>  Coxsackievirus A 21

<400>  5

Met Gly Ala Gln Val Ser Thr Gln Lys Thr Gly Ala His Glu Asn Gln 
1               5                   10                  15      


Asn Val Ala Ala Asn Gly Ser Thr Ile Asn Tyr Thr Thr Ile Asn Tyr 
            20                  25                  30          


Tyr Lys Asp Ser Ala Ser Asn Ser Ala Thr Arg Gln Asp Leu Ser Gln 
        35                  40                  45              


Asp Pro Ser Lys Phe Thr Glu Pro Val Lys Asp Leu Met Leu Lys Thr 
    50                  55                  60                  


Ala Pro Ala Leu Asn 
65                  


